CN103623451A - Technical method for forming sterile space at normal temperature - Google Patents
Technical method for forming sterile space at normal temperature Download PDFInfo
- Publication number
- CN103623451A CN103623451A CN201310521138.1A CN201310521138A CN103623451A CN 103623451 A CN103623451 A CN 103623451A CN 201310521138 A CN201310521138 A CN 201310521138A CN 103623451 A CN103623451 A CN 103623451A
- Authority
- CN
- China
- Prior art keywords
- space
- air
- temperature
- sterile
- technical method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a technical method for forming a sterile space at normal temperature. The technical method for forming the sterile space at normal temperature comprises the following steps: 1. performing instant sterilization on air through a high-temperature pipeline, wherein the temperature of the high-temperature pipeline is 120-200 DEG C, the diameter of the high-temperature pipeline is 2-20cm, and the length of the high-temperature pipeline is 1-4m; 2. sterilizing an overall airflow pipeline via the sterilized high-temperature air; 3. cooling the sterilized air to be at normal temperature through an air cooling heat dissipation method or a water bath method; 4. introducing sterile air from one side of a special space or a container continuously and enabling the sterile air to flow out of the other side of the space or the container, and then sterilizing the surface of the special space or the container so as to form a sterile space in the special space or the container. Through the technical method for forming the sterile space at normal temperature, the sterile space can be formed at normal temperature; the method is simple, the operation is easy, various environments can be adapted, the cost is low, a large sterile space can be formed and the sterile culture of strains or plants is performed in an open space.
Description
Technical field
The present invention relates to agricultural technology field, specifically a kind of sterilized space technical method that forms at normal temperatures.
Background technology
In agricultural production, the operation of plant tissue culture, the inoculation operation of edible fungi etc. all need to be carried out in gnotobasis, existing sterilized space produces principle: the 1st, and enclosed space is such as test tube or culture bottle employing autoclave sterilization generation sterilized space, the 2nd, open-sky technique platform adopts blower fan that air is sent out by " super cleaner " after-blow by superimposed group of microcellular foam lamella, form the continuously ultra-purify air laminar flow of dust-free sterile, i.e. so-called " effectively special air ", it has removed the dust that is greater than 0.3 μ m, fungus and bacterium spore etc.The flow velocity of ultra-purify air is 24～30m/min, this enough prevent near air may harass and the pollution that causes.Its shortcoming is that cleaner must regularly replace, and needs special equipment, and cost compare is high, can only be applied to certain operations link and can not be applied in production process.
Summary of the invention
In order to reduce the carrying cost of sterilized space, expand the range of application of sterilized space in agricultural production, the invention provides a kind of technical method that forms at normal temperatures sterilized space, can realize open space aseptic culture strain or plant.
Sterilized space of the present invention, because be mainly used in agricultural production, does not have specific (special) requirements to airborne dust, so adopt high temperature sterilization to produce filtrated air, need not filter.The steps include:
1, air is realized to instantaneously sterilising by the pipeline of high temperature; The pipeline of high temperature can obtain by electrical heating or other mode of heatings.Described high-temperature pipe temperature is 120～200 degrees Celsius; Described high-temperature pipe diameter is 2～20 centimetres, and length is 1～4 meter;
2, the high temperature air after sterilizing carries out sterilizing to whole airflow line; Described airflow line comprises high temperature sterilize pipeline and hot channel;
3, the air after sterilizing is cooled to room temperature by wind-cooling heat dissipating method or immersion method;
4, aseptic air continue from particular space or container one side passes into from space or container opposite side flows out, then particular space or vessel surface are carried out disinfection, thereby form aseptic space in particular space or containers.
The invention has the beneficial effects as follows and can form at normal temperatures sterilized space, method is simple, and easily operation, adapts to various environment, and cost is low, can form larger sterilized space, can realize and in open space, carry out aseptic culture strain or plant.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is further explained to explanation.
Embodiment 1
Form at normal temperatures a technical method that is applied to strain inoculation sterilized space, strain inoculation spatial volume is lower than one cubic metre.
1, air is realized to instantaneously sterilising by the pipeline of high temperature; The pipeline of high temperature can obtain by electrical heating or other mode of heatings.
Described pipe temperature is 200 degrees Celsius,
Described pipe diameter is that 2 centimetre lengths are 1 meter.
2, just the high temperature air after sterilizing carries out sterilizing to whole airflow line
3, the air after sterilizing is cooled to room temperature by wind-cooling heat dissipating method or immersion method;
What 4, aseptic air is continued passes into from opposite side and flows out from inoculating hood one side, then the inner surface of inoculating hood is carried out disinfection, thereby forms aseptic space in inoculating hood.
Embodiment 2
Form at normal temperatures and be applied to the take root technical method of sterilized space in stage of open tissue cultured seedling, open approximately 26～30 cubic metres of the device spatial volume of taking root.
It is characterized in that, step is as follows:
1, air is realized to instantaneously sterilising by the pipeline of high temperature; The pipeline of high temperature can obtain by electrical heating or other mode of heatings.
Described pipe temperature is 120 degrees Celsius,
Described pipe diameter is that 20 centimetre lengths are 4 meters.
2, just the high temperature air after sterilizing carries out sterilizing to whole airflow line
3, the air after sterilizing is cooled to room temperature by wind-cooling heat dissipating method or immersion method;
What 4, aseptic air is continued passes into from opposite side and flows out from open device one side of taking root, and then the inner surface surface of inoculating hood is carried out disinfection, thereby form aseptic space in the open device of taking root.
The above; it is only the preferably specific embodiment of the present invention; but protection scope of the present invention is not limited to this; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; according to technical scheme of the present invention and inventive concept thereof, be equal to replacement or changed, within all should being encompassed in protection scope of the present invention.
Claims (4)
1. form at normal temperatures a technical method for sterilized space, it is characterized in that, step is as follows:
(1), air is realized to instantaneously sterilising by the pipeline of high temperature;
(2), the high temperature air after sterilizing carries out sterilizing to whole airflow line;
(3), the air after sterilizing is cooled to room temperature by wind-cooling heat dissipating method or immersion method;
(4), aseptic air continue from particular space or container one side passes into from space or container opposite side flows out, then particular space or vessel surface are carried out disinfection, thereby form sterilized space in particular space or containers.
2. a kind of technical method that forms at normal temperatures sterilized space according to claim 1, is characterized in that: pipe temperature is 120～200 degrees Celsius.
3. a kind of technical method that forms at normal temperatures sterilized space according to claim 1 and 2, is characterized in that: described pipe diameter is 2～20 centimetres, and length is 1～4 meter.
4. a kind of technical method that forms at normal temperatures sterilized space according to claim 1 and 2, is characterized in that: described airflow line comprises high-temperature pipe and hot channel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310521138.1A CN103623451A (en) | 2013-10-27 | 2013-10-27 | Technical method for forming sterile space at normal temperature |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310521138.1A CN103623451A (en) | 2013-10-27 | 2013-10-27 | Technical method for forming sterile space at normal temperature |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103623451A true CN103623451A (en) | 2014-03-12 |
Family
ID=50205128
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310521138.1A Pending CN103623451A (en) | 2013-10-27 | 2013-10-27 | Technical method for forming sterile space at normal temperature |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103623451A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87204954U (en) * | 1987-03-28 | 1988-02-24 | 王德祥 | Electric heat sterilization inoculator |
| WO2003061840A1 (en) * | 2002-01-22 | 2003-07-31 | Talton James D Ph D | Method of pulsed laser assisted surface modification |
| JP2005131359A (en) * | 2003-10-08 | 2005-05-26 | Big Line:Kk | Sterilization device and sterilization method |
| US20060199172A1 (en) * | 2005-02-15 | 2006-09-07 | Fuji Photo Film Co., Ltd. | Laminated cell culture containing hepatocytes and method for detecting endocrine-like substance |
| CN1836735A (en) * | 2006-04-17 | 2006-09-27 | 东莞市星河生物科技有限公司 | Air cleaning system for edible mushroom inoculation |
| CN101249270A (en) * | 2008-03-28 | 2008-08-27 | 余兆丰 | Steam sterilizing system and sterilizing and cooling method thereof |
-
2013
- 2013-10-27 CN CN201310521138.1A patent/CN103623451A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87204954U (en) * | 1987-03-28 | 1988-02-24 | 王德祥 | Electric heat sterilization inoculator |
| WO2003061840A1 (en) * | 2002-01-22 | 2003-07-31 | Talton James D Ph D | Method of pulsed laser assisted surface modification |
| JP2005131359A (en) * | 2003-10-08 | 2005-05-26 | Big Line:Kk | Sterilization device and sterilization method |
| US20060199172A1 (en) * | 2005-02-15 | 2006-09-07 | Fuji Photo Film Co., Ltd. | Laminated cell culture containing hepatocytes and method for detecting endocrine-like substance |
| CN1836735A (en) * | 2006-04-17 | 2006-09-27 | 东莞市星河生物科技有限公司 | Air cleaning system for edible mushroom inoculation |
| CN101249270A (en) * | 2008-03-28 | 2008-08-27 | 余兆丰 | Steam sterilizing system and sterilizing and cooling method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NZ595944A (en) | Methods for cooling a carrier fluid of an electric power plant, electric power plants and cooling system | |
| CN103623451A (en) | Technical method for forming sterile space at normal temperature | |
| CN209122940U (en) | A kind of health-caring capsule bactericidal unit | |
| CN204385199U (en) | A kind of inoculating device of fermentation tank | |
| CN204208073U (en) | A kind of Cordyceps disinfection tank | |
| CN202819039U (en) | Edible fungi positive pressure germ-free purifying inoculation production line | |
| CN202440293U (en) | Isolator air treatment system | |
| CN102379212A (en) | Precooling system and precooling method after sterilization of edible mushroom cultivation base stock | |
| CN203469068U (en) | Rotary sterilization system with negative pressure cooling device | |
| CN103493732B (en) | A kind of controllable temperature macro suspension culture apparatus with Spore adhesion parts | |
| CN202682409U (en) | Laminar flow air inlet device | |
| CN105766455A (en) | Sterile and energy-saving experimental apparatus for plant seedling raising | |
| JP2011050611A (en) | Packaged boiled noodle manufacturing apparatus, and packaged boiled noodle manufacturing method | |
| CN203289937U (en) | Simple steam inoculator | |
| CN203233733U (en) | Sterile inoculation chamber | |
| CN204350743U (en) | A kind of stevia incubator | |
| CN204702619U (en) | Purified water stores pasteurization system | |
| CN103283490A (en) | Simple steam inoculator | |
| CN203530323U (en) | Fermentation tank | |
| CN204281753U (en) | A kind of fungus culture vessel device for Bioexperiment teaching | |
| CN203575175U (en) | Edible fungus conveying belt type sterilization device | |
| CN203281321U (en) | Degerming box for laboratories | |
| CN103014084A (en) | Steam condensate recycling process and device of amino acid fermentation system | |
| RU2012102637A (en) | THERAPEUTIC AND PREVENTIVE PRODUCTS AND METHOD FOR PRODUCING THEM | |
| CN103432606A (en) | Rotary sterilization system with negative pressure cooling device and sterilization method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140312 |