CN103614281A - Lipopeptide fermentation apparatus through foam circulation, and process - Google Patents
Lipopeptide fermentation apparatus through foam circulation, and process Download PDFInfo
- Publication number
- CN103614281A CN103614281A CN201310476843.4A CN201310476843A CN103614281A CN 103614281 A CN103614281 A CN 103614281A CN 201310476843 A CN201310476843 A CN 201310476843A CN 103614281 A CN103614281 A CN 103614281A
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- tank
- froth breaking
- fermentation
- fermentor
- withdrawing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
Abstract
The present invention discloses a lipopeptide fermentation process apparatus through foam circulation. The apparatus comprises a fermentation tank (1), a defoaming tank (2), a recovery tank (3) and a peristaltic pump (4), wherein the top of the air lifting type fermentation tank (1) is connected with the defoaming tank (2) through a pipeline and a valve, the top of the defoaming tank (2) is connected with the top of the recovery tank (3) through a pipeline and a valve, the defoaming tank (2) is connected with an air exhaust pipeline A, the recovery tank (3) is connected with an air exhaust pipeline B, and the peristaltic pump (4) is concurrently connected with the fermentation tank (1), the defoaming tank (2) and the recovery tank (3) through pipelines and valves. According to the present invention, recycling of lipopeptide fermentation foams is achieved, lipopeptide fermentation loss is avoided, the yield is increased, the investment is less, and the operation is simple.
Description
Technical field
The present invention relates to a kind of fermentation unit and technique, be specifically related to a kind of foam circulation lipopeptid fermentation unit and technique.
Background technology
Lipopeptid is a kind of bio-surfactant that fermentation of bacillus subtilis produces, and molecule is comprised of fat hydrocarbon chain two portions of hydrophilic peptide bond and oleophylic, and due to its special chemical constitution and amphiphilic molecules structure, lipopeptide biosurfactant is at medicine.There is important application prospect in the field such as microbe oil production, environmental improvement.The production of existing lipopeptid is mainly microbe fermentation method, owing to producing a large amount of foams in fermenting process, and the effect that existing machinery froth breaking and defoamer froth breaking all can not be obtained, the reason that causes this phenomenon is mainly that the process unit design of foam circulation lipopeptid fermentation is unreasonable, the a large amount of foams that produce in fermenting process can not be discharged in time, lipopeptid fermentation conversion rate is low, causes lipopeptid class leavened prod suitability for industrialized production in a large number.
Summary of the invention
In order to overcome the froth breaking difficult problem in Production by Microorganism Fermentation lipopeptid process, the invention provides a kind of foam circulation lipopeptid fermentation unit and technique.This process unit can be discharged a large amount of foams that produce in fermenting process in time, has not only realized the fermentative production of lipopeptid, and can greatly improve lipopeptid fermentation conversion rate.This process unit is simple in structure, and less investment is simple to operate, and suitability for industrialized is promoted the use of.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of foam circulation lipopeptid zymotechnique device, comprise: fermentor tank (1), froth breaking tank (2), withdrawing can (3), peristaltic pump (4), airlift fermentor (1) top is connected froth breaking tank (2) with valve through pipeline, froth breaking tank (2) top is connected withdrawing can (3) top with valve through pipeline, froth breaking tank (2) connects vent line A, and withdrawing can (3) connects vent line B; Peristaltic pump (4) is connected fermentor tank (1), froth breaking tank (2), withdrawing can (3) through pipeline and valve simultaneously.
Preferably, described fermentor tank (1) is airlift fermentor.Valve is arranged on pipeline.
In the present invention, the foam that airlift fermentor produces enters froth breaking tank by upflow tube from froth breaking tank top with certain pressure, the gas producing after lather collapse enters withdrawing can by venting port and the pipeline of froth breaking tank top, gas is removed in the top vent discharge by withdrawing can, and the recovery liquid process peristaltic pump circulation that the foam liquefier that froth breaking tank produces and withdrawing can produce enters fermentor tank and continues fermentation.Whole foam working cycle is that malleation is carried out, whole system can be in fermentor tank sterilization process sterilization simultaneously, guaranteed the aseptic operation of whole process.
A kind of foam circulation lipopeptid zymotechnique device the present invention relates to, comprises that the fermentor tank of employing is airlift fermentor, is based on airlift fermentor good leak tightness, the microbiological contamination problem of having avoided foam top set to cause; Fermentor tank control pressure 0.05MPa, froth breaking tank control pressure 0.01～0.02MPa, design froth breaking tank volume is 0.5～1.0% of fermentor tank volume, be for build-up of pressure poor, the lipopeptid foam that fermentation is produced easily breaks, and reduces floor space, the microbiological contamination problem of simultaneously avoiding hypotony to cause, froth breaking pot bottom is provided with leakage fluid dram, and exclusive segment foam solution guarantees the efficient operation of lipopeptid secondary metabolism in good time; The volume of withdrawing can is 0.05～0.1% of fermentor tank volume, and control pressure is 0.01～0.02Mpa, is to have little time the part foam that froth breaking is escaped and to be removed in order to reclaim because of froth breaking tank; Adopting peristaltic pump circulation foam liquefier is because peristaltic pump does not directly contact with material, and can move continuously, has avoided microbiological contamination, and peristaltic pump can be realized liquid low pressure to high pressure, the harm of having avoided pressure difference to cause.
The invention has the beneficial effects as follows, realized the recycling of lipopeptid barm, avoided lipopeptid fermentation loss, improved output; Less investment, simple to operate.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Embodiment
The present invention is described further in connection with accompanying drawing for following examples.
As shown in Figure 1, a kind of foam circulation lipopeptid zymotechnique device is combined by airlift fermentor (1), froth breaking tank (2), withdrawing can (3), peristaltic pump (4).Implement the present invention, the lipopeptid foam that airlift fermentor (1) produces enters froth breaking tank (2) top through pipeline and valve, the gas producing after froth breaking enters withdrawing can (3) top by froth breaking tank (2) overhead line and valve, and gas is discharged through withdrawing can top vent line B subsequently.The liquid that the liquefaction foam that froth breaking tank produces and withdrawing can produce is squeezed into fermentor tank (1) through peristaltic pump (4).The partial liquefaction foam that froth breaking tank produces can be discharged by A pipeline.
Concrete operations:
10m
3airlift fermentor (1) sterilizing: open valve 5,6,7,8,9,10, system high temperature autoclave sterilization, 115 ℃, 15min.Be cooled to leavening temperature, valve-off 9, adjusts airlift fermentor (1) pressure 0.05MPa by regulated valve 5,7, and 100L froth breaking tank (2) pressure is 0.02MPa, and 10L withdrawing can (3) pressure is 0.02MPa, and gas is discharged by channel B.After having inoculated, start fermentation, until lipopeptid foam to airlift fermentor (1) top, enter froth breaking tank (2), treat liquid level to half, and open peristaltic pump (4), the speed producing according to liquid is adjusted the rotating speed of peristaltic pump (4) in good time, by liquid continuous squeeze into fermentor tank (1), treat the lipopeptid fermentation later stage, valve-off 6,8,10 and peristaltic pump (4), Open valve 9, discharges lipopeptid foam liquefier by passage A and collects purification.The whole process operation of system is normal, and foam liquefying speed is fast, does not occur that foam solution is from froth breaking tank spillover.
Obviously, the above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here cannot give all embodiments exhaustive.Every still row in protection scope of the present invention of apparent variation that technical scheme of the present invention extends out or change that belong to.
Claims (2)
1. a foam circulation lipopeptid fermentation unit, it is characterized in that, comprise: fermentor tank (1), froth breaking tank (2), withdrawing can (3), peristaltic pump (4), airlift fermentor (1) top is connected froth breaking tank (2) with valve through pipeline, froth breaking tank (2) top is connected withdrawing can (3) top with valve through pipeline, froth breaking tank (2) connects vent line A, and withdrawing can (3) connects vent line B; Peristaltic pump (4) is connected fermentor tank (1), froth breaking tank (2), withdrawing can (3) through pipeline and valve simultaneously.
2. the technique of fermentation unit according to claim 1, it is characterized in that, fermentor tank adopts airlift fermentor, control pressure is 0.05MPa, the volume of froth breaking tank is 0.5～1.0% of fermentor tank volume, the volume of withdrawing can is 0.05～0.1% of fermentor tank volume, and control pressure is 0.01～0.02MPa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310476843.4A CN103614281A (en) | 2013-10-12 | 2013-10-12 | Lipopeptide fermentation apparatus through foam circulation, and process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310476843.4A CN103614281A (en) | 2013-10-12 | 2013-10-12 | Lipopeptide fermentation apparatus through foam circulation, and process |
Publications (1)
| Publication Number | Publication Date |
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| CN103614281A true CN103614281A (en) | 2014-03-05 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310476843.4A Pending CN103614281A (en) | 2013-10-12 | 2013-10-12 | Lipopeptide fermentation apparatus through foam circulation, and process |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5362635A (en) * | 1990-09-21 | 1994-11-08 | Ajinomoto Co., Inc. | Process for the production of an amino acid using a fermentation apparatus |
| US5476573A (en) * | 1991-11-22 | 1995-12-19 | Ajinomoto Co., Inc. | Apparatus for defoaming and controlling aerobic culture fermentation |
| CN201520758U (en) * | 2009-09-30 | 2010-07-07 | 中国科学院等离子体物理研究所 | Foam return fermentation system |
| CN203582858U (en) * | 2013-10-12 | 2014-05-07 | 北京市捷博特能源技术有限公司 | Foam circulation lipopeptide fermentation process device |
-
2013
- 2013-10-12 CN CN201310476843.4A patent/CN103614281A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5362635A (en) * | 1990-09-21 | 1994-11-08 | Ajinomoto Co., Inc. | Process for the production of an amino acid using a fermentation apparatus |
| US5476573A (en) * | 1991-11-22 | 1995-12-19 | Ajinomoto Co., Inc. | Apparatus for defoaming and controlling aerobic culture fermentation |
| CN201520758U (en) * | 2009-09-30 | 2010-07-07 | 中国科学院等离子体物理研究所 | Foam return fermentation system |
| CN203582858U (en) * | 2013-10-12 | 2014-05-07 | 北京市捷博特能源技术有限公司 | Foam circulation lipopeptide fermentation process device |
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Application publication date: 20140305 |