CN103609754A - Paeonia ostii seed oil with oxidative stability and preparation method thereof - Google Patents
Paeonia ostii seed oil with oxidative stability and preparation method thereof Download PDFInfo
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- CN103609754A CN103609754A CN201310648247.XA CN201310648247A CN103609754A CN 103609754 A CN103609754 A CN 103609754A CN 201310648247 A CN201310648247 A CN 201310648247A CN 103609754 A CN103609754 A CN 103609754A
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Abstract
The invention discloses paeonia ostii seed oil with oxidative stability. The paeonia ostii seed oil is a mixture of a natural extract which is formed by compounding extracted fresh paeonia ostii seed oil with beta-carotene, lycopene, a rosemary extract and citric acid. The mixture comprises the following components: 0.02-0.06% of beta-carotene, 0.03-0.06% of lycopene, 0.03-0.07% of rosemary extract, 0.02-0.10% of citric acid and the balance of paeonia ostii seed oil. The invention also discloses a preparation method of the paeonia ostii seed oil. The method comprises the following steps: extracting the paeonia ostii seed oil; respectively preparing mother liquid of the citric acid, the beta-carotene, the lycopene and the rosemary extract; adding the citric acid mother liquid; adding the beta-carotene mother liquid and the lycopene mother liquid; and standing and refrigerating, and adding the rosemary extract mother liquid. The storage capacity of the paeonia ostii seed oil is greatly improved by compounding four natural products, so that the paeonia ostii seed oil has the oxidative stability, and the quality guarantee period of the paeonia ostii seed oil is prolonged.
Description
Technical field
The present invention relates to red seed oil of a kind of phoenix and preparation method thereof, particularly relate to red seed oil of a kind of oxidation stability phoenix and preparation method thereof.
Background technology
Feng Dan (Paeoniaostii T.Hong et J.X.Zhang), i.e. Tongling tree peony, former plant is poplar seguin argyreia herb.Because mainly originating in Nanling, Anhui Province Western Hills and Feng huangshan Mountain, Tongling one band, therefore be referred to as Feng Dan, belong to Ranunculaceae Paeonia shrub.Because of conditions such as its unique weather, soil, the tree peony that produce in Tongling has characteristic.Its flower mostly is single-lobe, is white in color, and has pinkly, and flower great Ye is emerald green, and fragrance is attacked people; Especially its rhizome is round and sturdy, and the thin meat of skin is thick, and storage is never degenerated for a long time; Section mealiness foot; Color of the leather is golden yellow, has bright star point, and gloss is as copper.Within 2006, Feng Dan is listed in " national geography sign protection product ".
The red seed oil of phoenix is a kind of edible oil extracting from the red seed of phoenix.Research shows, in the red seed oil of phoenix, contains 89.00% unrighted acid, wherein mainly take linoleic acid (33.34%) and leukotrienes (54.81%) as main.The red seed oil of phoenix is in storage, processing and the process of circulation, because being subject to O in air
2, the factor effect such as metal ion, illumination oxidative rancidity very easily, cause variable color or spoiled and can generate harmful substance, be unfavorable for the utilization of the red seed oil of phoenix.
At present, in grease, adding antioxidant has become and in grease processing, has prevented the most economical and simple method that Oxidation of Fat and Oils is rotten.Although artificial synthetic antioxidant action effect is remarkable, because safety problem is used by the restriction of a plurality of countries.
In recent years, increasing natural is developed.Natural can directly extract from animals and plants, safe, can not only prevent Oxidation of Fat and Oils, also can prevent the harm of people's interior free yl, and the various degenerative disorders that delay senility and cause, as cancer, diabetes etc.
In general, synergistic function between composite antioxidant can strengthen the antioxygenic property of antioxidant, its effect is better than single antioxidant, but, between different composite antioxidant, when there is synergistic function, also there is certain homogeneity, antagonistic effect, be that compound use can not strengthen antioxidant effect, or the enhancing rate of antioxidant effect and cost imbalance, business use value be there is no.This situation is especially when several or multiple antioxidant compound use, and along with increasing of antioxidant kind, its antioxidant effect no longer includes lifting.The mechanism of action of different antioxidants has difference, and the link of ending in Oxidation of Fat and Oils process is different, has the impact of the factors such as antagonism between composite antioxidant, and composite antioxidant needs repeatedly proportioning can realize the lifting of antioxidant effect.
Summary of the invention
The invention provides the red seed oil of a kind of oxidation stability phoenix, the red seed oil of this phoenix has specifically added citric acid and 3 kinds of naturals---beta carotene, lycopene, Rosmarinus officinalis extract in the red seed oil of the fresh phoenix obtaining by extraction solvent.Can significantly improve the antioxygenic property of the red seed oil of phoenix.And all additives are in GB, to allow the food additives of interpolation, and addition also meets the relevant regulations of GB.Be for beta carotene No. CAS wherein: 7235-40-7, is for No. CAS of lycopene: 502-65-8, is for No. CAS of Rosmarinus officinalis extract: 84604-14-8, is for No. CAS of citric acid: 77-92-9.
The red seed oil of a kind of oxidation stability phoenix, comprises that mass fraction is 0.02%-0.06% beta carotene, 0.03%-0.06% lycopene, 0.03%-0.07% Rosmarinus officinalis extract and 0.01-0.10% citric acid, and surplus is the red seed oil of phoenix.
The red seed oil of a kind of oxidation stability phoenix, formula is preferably comprised of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.
Another object of the present invention is to provide the preparation method of the red seed oil of above-mentioned oxidation stability phoenix, and described method is simple, the red seed oil oxidation stability of phoenix promotes, is easy to suitability for industrialized production to some extent than commonsense method.
A preparation method for the red seed oil of stability phoenix, comprises following detailed step:
(1) extraction of the red seed oil of phoenix: pluck the red seed of phoenix, the red seed of the dry phoenix of pulverizing, adopts tradition leaching or squeezing to put forward oily method and extract the red seed oil of phoenix;
(2) prepare additive mother liquor: the citric acid mother liquor, the beta carotene mother liquor of 0.5%-5%, the Rosmarinus officinalis extract mother liquor of the lycopene mother liquor of 0.5%-5% and 0.5%-5% that with the red seed oil of phoenix preparation mass fraction, are 0.5%-5% respectively;
(3) add citric acid mother liquor: take out citric acid mother liquor, join in the red seed oil of phoenix, concussion 10-30min, obtains mixture I;
(4) add beta carotene and lycopene mother liquor: take out respectively beta carotene mother liquor and lycopene mother liquor, join in mixture I, concussion 10-30min, obtains mixture II;
(5) standing refrigeration: by the standing refrigeration of mixture II 24-48h;
(6) add Rosmarinus officinalis extract mother liquor: get Rosmarinus officinalis extract mother liquor and join in the mixture II after standing, concussion 10-30min, obtains the mixture of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix pellet seed oil.
Beneficial effect of the present invention
The beneficial effect of the red seed oil of oxidation stability phoenix
Research finds, in the red seed oil of phoenix, saturated fatty acid accounts for 10.77%, unrighted acid accounts for 89.00%.Wherein main fatty acid is leukotrienes 54.81%, linoleic acid 33.34%, palmitic acid 8.07%, stearic acid 2.29%.The oxidization time of different aliphatic acid is different with speed, and the antioxidant of different antioxidation mechanism also exists very large difference to the antioxidant effect of different aliphatic acid.The aliphatic acid of the red seed oil of phoenix is mainly linolenic and linoleic, in grease lixiviate and storage, is very easily oxidized, and forms rancidity beastly, affects the quality of product, harm user's health.
The current method that prevents Oxidation of Fat and Oils is to add antioxidant, but in recent years, people conduct extensive research for the safety issue of artificial synthetized oxidation preventive agent.The World Health Organization (FA0/WHO), European Community's protection of the child tissue (HACSG), Britain biological industry association (BIBRA), Japan, the government of the U.S. and the research of tissue show; synthetized oxidation preventive agent has many side effects; as the liver of human body, spleen, lung are all had to adverse effect, can bring out malignant tumour etc.So FDA (Food and Drug Adminstration) (FDA) suggestion is left out BHT in it is generally acknowledged safe material; The relevant hygiene department of Japan once made the conclusion that bans use of BHA after deliberation; For TBHQ, although having ratified its use ，Dan European Union, Japan and other countries in some grease, a few countries such as the U.S. think its toxicological test data imperfection, not yet approval is used.In a word, there is uneasy psychology to artificial synthetized oxidation preventive agent in people.
The red seed oil of a kind of stability phoenix provided by the invention, adopted several natural anti-oxidation extracts composite, obtained the red seed oil mixture of a kind of phoenix that contains several natural anti-oxidation extracts, this mixture is formulated according to fatty acid component and the oxidation characteristic thereof of the red seed oil of phoenix.
First, add citric acid.Citric acid can effectively be removed the Oxidation of Fat and Oils that metal ion causes, in the red seed oil of phoenix, contain micro-metal ion, these metal ions one are to be derived from metallic activating enzymes or its catabolite, the 2nd, be derived from contacting metal container in phoenix red seed oil refining and food processing.Heavy metal is those metals that have two valencys or higher valence state and have suitable redox potential between them particularly, can shorten the time of chain reaction induction period, accelerate the speed of lipoid substance oxidation.Existing research is verified, when the porphyrin ring complex concentration of metal and slaine is 10-8mol/L, produces strong oxidation effect.Some can be used as antioxidant containing complexing agents of oxygen coordination atom, because of after they and complexing of metal ion, can reduce redox potential, and the oxidation state of stable metal ion suppresses the enzymatic oxidation effect of metal ion effectively.Add after citric acid, removed the oxidation factors such as metal ion in the red seed oil of phoenix, make beta carotene and lycopene antioxidant effect remarkable.
Secondly, add beta carotene and lycopene, two of carotenoids kinds of materials is compound, and these two kinds of materials are to add according to the ratio of contained linoleic acid plus linolenic acid in the red seed of phoenix.Excited oxygen is buried in oblivion agent simple substance oxygen has two kinds to have energy state, and the one, singlet, i.e. excitation state (1O2); Another is triplet state (3O2), i.e. ground state.Singlet is that excited oxygen can be oxidized to hydroperoxides by lipoid substance, is one of reason of Oxidation of Fat and Oils.Carotenoid material can change singlet oxygen (excitation state) into triplet state (ground state) oxygen under hypoxemia pressure, has eliminated the existence of singlet oxygen, thus the antioxidation of playing.Simultaneously; lycopene is as powerful antioxidant; energy quenching singlet oxygen and removing free radical; effectively in Cell protection, various large biological molecules are not subject to the damage of free radical; lycopene stops the ability of ultra violet radiation simultaneously in addition; prevent the impact of illumination on the oxidation of the red seed oil of phoenix, stop the red seed oil of phoenix that photosensitized oxidation occurs in storage.
Finally, add Rosmarinus officinalis extract, be for No. CAS: 84604-14-8.Domestic and foreign literature data shows, the main terpene of antioxidant content, phenols and acid in Rosmarinus officinalis extract.Owing to containing multiple anti-oxidant active ingredient and multiple antioxidation mechanism, cause rosemary, Xue MingRosma rinus officinalis to there is efficient and non-oxidizability widely.Generally believe at present, rosemary, Xue MingRosma rinus officinalis antioxidation mechanism is mainly its energy quencher creating singlet oxygen by using, removes free radical, cuts off the chain reaction of lipoid autoxidation, chelated metal ions etc.In Rosmarinic acid, the composition of reproducibility, as phenolic hydroxyl group, unsaturated double-bond and acid etc., has antioxidation during individualism, has synergy while combining.As can be seen here, the antioxidant effect of Rosmarinus officinalis extract is fine, there is diversity, but, the price of rosemary, Xue MingRosma rinus officinalis is higher, if start, add, antioxidant content in rosemary, Xue MingRosma rinus officinalis is together with citric acid, beta carotene is removed the oxidation factor in whole grease system together with lycopene, cause the inefficacy of rosemary, Xue MingRosma rinus officinalis self polyphenoils, under the condition of equal addition, the antioxidant effect of Rosmarinus officinalis extract can significantly reduce, the present invention is by adding citric acid, standing 24-48h after beta carotene and lycopene, citric acid and beta carotene and lycopene are played one's part to the full, make price lower, widely used citric acid consumes while being oxidized factor in removing the red seed oil systems of phoenix, and then add successively effect better, the antioxidant content that price is more expensive, give full play to the effect of each antioxidant content, when having avoided the antioxidant of same mechanism, use, under the constant condition of addition, increased the action effect of antioxidant, strengthened the red seed oil oxidation stability of phoenix.
By accelerating contrast test, in embodiment 1, embodiment group is accelerated experiment under the condition of 63 ± 1 ℃, deposits after 18 days, and its peroxide value is only about 15% of the red seed oil group of phoenix (control group 12), as can be seen here, the keeping quality of the red seed oil mixture of this phoenix has promoted nearly 7 times than the red seed oil of phoenix, meanwhile, and according to other control groups, can find out V
c, V
eas an alternative, or lack lycopene or beta carotene and all can make the red seed oil oxidation stability of phoenix reduce, storage time also reduces accordingly.
The beneficial effect of the red seed oil method of preparation oxidation stability phoenix
The invention provides a kind of method that improves the collaborative efficiency of natural, the method in turn includes the following steps: extract the red seed oil of phoenix, the preparation of additive mother liquor, add beta carotene and lycopene, standing mixt, add Rosmarinus officinalis extract, prepare by this method the red seed oil of phoenix with oxidation stability of the present invention, can make its oxidation stability further be promoted, embodiment group and control group 1 in embodiment 1, the experimental data of control group 11 can be found out, the peroxide value of the acceleration of embodiment group after 18 days is starkly lower than control group 1 and control group 11.
This mixture of the red seed oil of phoenix with oxidation stability provided by the invention does not change the red seed original composition of oil of phoenix and color, smell and taste, all additives all belong to the food additives of national regulation, and addition meets national regulation, can effectively extend the shelf-life of the red seed oil of phoenix, solve in the processing of the red seed oil product of phoenix and storage the easily problem of oxidation deterioration, for the red seed oil product of phoenix enters consumers in general market, provide useful help.
Preparation method of the present invention is simple, and cost is lower, is easy to suitability for industrialized production, and the red seed oil of phoenix of preparing by the method has good oxidation stability.
Accompanying drawing explanation
Fig. 1 is preparation method's of the present invention schematic flow sheet.
The specific embodiment
Embodiment 1
The red seed oil of a kind of stability phoenix, is comprised of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.
Wherein the red seed of phoenix originates from Tongling, Anhui Province Feng huangshan Mountain, is for No. CAS of beta carotene: 7235-40-7, is for No. CAS of lycopene: 502-65-8, is for No. CAS of Rosmarinus officinalis extract: 84604-14-8, is for No. CAS of citric acid: 77-92-9.
A preparation method for the red seed oil of stability phoenix, comprises the steps:
1. pluck the red seed of phoenix: choose the red seed of phoenix of full grains, clean the earth on the red seed of phoenix, dry under natural conditions.
2. pulverize dry: the red seed of clean phoenix is put into Chinese herbal medicine atomizer and pulverize, cross 40 mesh sieves, the dry red powder of seeds of phoenix that obtains.
3. extract the red seed oil of phoenix, adopt benzinum lixiviation process to extract the red seed oil of phoenix.
4. separating and filtering: after having extracted, the mixed liquor with the red powder of seeds of Filter paper filtering phoenix with benzinum, filter paper lower floor obtains benzinum filtrate, and filter paper upper strata obtains the phoenix pellet powder of seeds of degreasing.
5. drying under reduced pressure: adopting Rotary Evaporators is 50kPa in vacuum, and under the condition that temperature is 40 ℃, rotary evaporation also reclaims benzinum, until benzinum is distilled out of completely, obtains the red seed oil of phoenix.
6. in four brown bottles, accurately take respectively the red seed oil of 9.9000g phoenix, stand-by.
7. the preparation of additive mother liquor: accurately take citric acid, beta carotene, lycopene, each 0.1000g of Rosmarinus officinalis extract with assay balance, join successively respectively in the brown bottle that in step 6, four contain the red seed oil of 9.9000g phoenix, and sealing, put into 20 ℃ of water-baths concussion case lucifuges concussion 30min it is uniformly dispersed, be made into respectively mass fraction and be 1% citric acid mother liquor, 1% beta carotene mother liquor, 1% lycopene mother liquor and 1% Rosmarinus officinalis extract mother liquor.Be stored under 4 ℃ of air-proof conditions, standby.
8. add citric acid mother liquor: take out 2mL1% citric acid mother liquor, join in the red seed oil of 89mL phoenix, 20 ℃ of Water Unders are bathed concussion 10min, and it is uniformly dispersed, and obtain the mixture of the red seed oil of citric acid and phoenix, called after mixture I.
9. add beta carotene and lycopene mother liquor: take out respectively the beta carotene mother liquor of 2mL1% and the lycopene mother liquor of 3mL1%, join in the mixture I that step 8 obtains, 20 ℃ of Water Unders are bathed concussion 30min, it is uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene and phoenix, called after mixture II.
10. standing mixt II: mixture II is refrigerated to 24h under the condition of 4 ℃.
11. add Rosmarinus officinalis extract mother liquors: get respectively 4mL1% Rosmarinus officinalis extract mother liquor and join in the mixture II after standing, 20 ℃ of Water Unders are bathed concussion 30min, it is uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, for the present invention obtains having the red seed oil of phoenix of stability.
Product detects and contrast experiment:
Embodiment group: according to the step in the present embodiment, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture is comprised of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.By this group called after embodiment group.
Control group 1: remove the step 10 in the present embodiment group, other steps are with embodiment group, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture is comprised of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.By this group called after control group 1.
Control group 2: by the step of embodiment group, the part of contained Rosmarinus officinalis extract changes the red seed oil of phoenix of equivalent weight into, other are with the step in embodiment group, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture is comprised of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene and 0.02% citric acid, surplus is the red seed oil of phoenix.By this group called after control group 2.
Control group 3: by the step of embodiment group, the part of contained beta carotene and lycopene changes the red seed oil of phoenix of equivalent weight into, other are with the step in embodiment group, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture forms 0.04% Rosmarinus officinalis extract and 0.02% citric acid by the material of following mass percent, and surplus is the red seed oil of phoenix.By this group called after control group 3.
Control group 4: by the step of embodiment group, the part of contained citric acid changes the red seed oil of phoenix of equivalent weight into, other are with the step in embodiment group, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture is comprised of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene and 0.04% Rosmarinus officinalis extract, surplus is the red seed oil of phoenix.By this group called after control group 4.
Control group 5: by the step of embodiment group, the part of contained citric acid, lycopene and beta carotene changes the red seed oil of phoenix of equivalent weight into, other are with the step in embodiment group, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture is comprised of the material of following mass percent: 0.04% Rosmarinus officinalis extract, surplus is the red seed oil of phoenix.By this group called after control group 5.
Control group 6: by the step of embodiment group, the part of contained beta carotene changes the lycopene of equivalent weight into, other are with the step in embodiment group, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture is comprised of the material of following mass percent: 0.05% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.By this group called after control group 6.
Control group 7: by the step of embodiment group, the part of contained lycopene changes the beta carotene of equivalent weight into, other are with the step in embodiment group, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture is comprised of the material of following mass percent: 0.05% beta carotene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.By this group called after control group 7.
Control group 8: by the step of embodiment group, the part of contained beta carotene, lycopene changes the red seed oil of phoenix of equivalent weight into, other are with the step in embodiment group, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture is comprised of the material of following mass percent: 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.By this group called after control group 8.
Control group 9: by the step of embodiment group, the part of contained citric acid changes the vitamin C of equivalent weight into, other are with the step in embodiment group, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture is comprised of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene and 0.04% Rosmarinus officinalis extract and 0.02% vitamin C, surplus is the red seed oil of phoenix.By this group called after control group 9.
Control group 10: by the step of embodiment group, the part of contained Rosmarinus officinalis extract changes the vitamin E of equivalent weight into, other are with the step in embodiment group, the mixture of preparing the red seed oil of 2 parts of 100mL phoenix, this mixture is comprised of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene and 0.04% Rosmarinus officinalis extract and 0.02% vitamin E, surplus is the red seed oil of phoenix.By this group called after control group 10.
Control group 11: by the step of embodiment group, the beta carotene mother liquor of 2mL1% citric acid mother liquor, 2mL1%, the lycopene mother liquor of 3mL1% join in the red seed oil of 89mL phoenix together with 4mL1% Rosmarinus officinalis extract mother liquor, 20 ℃ of Water Unders are bathed concussion 10min, it is uniformly dispersed, obtain the mixture of the red seed oil of citric acid and phoenix, this mixture is comprised of the material of following mass percent: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix, by this group called after control group 11.
Control group 12: get the red seed oil of undressed 100mL phoenix called after control group 12.
By the uncovered digital display thermostatic drying chamber that is placed in 63 ± 1 ℃ of the red seed oil mixture of the phoenix of embodiment group and all control groups, every 2 days sampling analysis measuring peroxide values, observe the quality of sample under 63 ± 1 ℃ of environment and change.The assay method of peroxide value is by carrying out with reference to GB/T5538-2005.
The situation of change of its peroxide value is as shown in table 1, and abscissa is the mean value of each group peroxide value, and ordinate is the number of days under 63 ± 1 ℃ of environment.(meq/kg of peroxide value unit)
Table 1, the peroxide value Changing Pattern of embodiment group and control group
Table 1 is continuous, the peroxide value Changing Pattern of embodiment group and control group
Data in form can find out, the rising that adopts method in embodiment 1 and addition can effectively delay the red seed oil of phoenix peroxide value in experimental period, and 1 group of embodiment is significantly better than other groups.Obviously poor than of the present invention by changing adding method or changing the antioxidant effect of other naturals.The red seed oil of phoenix obtaining by method of the present invention can be under the condition of 63 ± 1 ℃, in 20 days, effectively controls the oxidation of the red seed of phoenix, and its peroxide value of 20 days is only 13.4311meq/kg, has greatly promoted the keeping quality of the red seed oil of phoenix.
Embodiment 2
The red seed oil of a kind of stability phoenix, is comprised of the material of following mass percent: 0.03% beta carotene, 0.04% lycopene, 0.04% Rosmarinus officinalis extract and 0.07% citric acid, surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. pluck the red seed of phoenix: choose the red seed of phoenix of full grains, clean the earth on the red seed of phoenix, dry under natural conditions.
2. pulverize dry: the red seed of clean phoenix is put into Chinese herbal medicine atomizer and pulverize, cross 100 mesh sieves, the dry red powder of seeds of phoenix that obtains.
3. extract the red seed oil of phoenix, adopt lixiviation process to extract the red seed oil of phoenix.
4. separating and filtering: after having extracted, the mixed liquor with the red powder of seeds of Filter paper filtering phoenix with benzinum, filter paper lower floor obtains benzinum filtrate, and filter paper upper strata obtains the phoenix pellet powder of seeds of degreasing.
5. drying under reduced pressure: adopting Rotary Evaporators is 50kPa in vacuum, and under the condition that temperature is 40 ℃, rotary evaporation also reclaims benzinum, until benzinum is distilled out of completely, obtains the red seed oil of phoenix.
6. in four brown bottles, accurately take respectively the red seed oil of 9.9000g phoenix, stand-by.
7. the preparation of additive mother liquor: accurately take citric acid, beta carotene, lycopene, each 0.0500g of Rosmarinus officinalis extract with assay balance, join successively respectively in the brown bottle that in step 6, four contain the red seed oil of 9.9500g phoenix, and sealing, put into 20 ℃ of water-baths concussion case lucifuges concussion 10min it is uniformly dispersed, be made into respectively mass fraction and be 0.5% citric acid mother liquor, 0.5% beta carotene mother liquor, 0.5% lycopene mother liquor and 0.5% Rosmarinus officinalis extract mother liquor.Be stored under 0 ℃ of air-proof condition, standby.
8. add citric acid mother liquor: take out 14mL1% citric acid mother liquor, join in the red seed oil of 64mL phoenix, 20 ℃ of Water Unders are bathed concussion 10min, and it is uniformly dispersed, and obtain the mixture of the red seed oil of citric acid and phoenix, called after mixture I.
9. add beta carotene and lycopene mother liquor: take out respectively the beta carotene mother liquor of 6mL0.5% and the lycopene mother liquor of 8mL0.5%, join in the mixture I that step 8 obtains, 4 ℃ of Water Unders are bathed concussion 10min, it is uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene and phoenix, called after mixture II.
10. standing mixt II: mixture II is refrigerated to 48h under the condition of 4 ℃.
11. add Rosmarinus officinalis extract mother liquors: get respectively 8mL0.5% Rosmarinus officinalis extract mother liquor and join in the mixture II after standing, 20 ℃ of Water Unders are bathed concussion 10min, it is uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, the red seed oil of the phoenix with oxidation stability obtaining for the present invention.
Embodiment 3
The red seed oil of a kind of stability phoenix, is comprised of the material of following mass percent: 0.03% beta carotene, 0.04% lycopene, 0.03% Rosmarinus officinalis extract and 0.10% citric acid, surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. pluck the red seed of phoenix: choose the red seed of phoenix of full grains, clean the earth on the red seed of phoenix, dry under natural conditions.
2. squeezing is fuel-displaced: adopt the fuel-displaced equipment of squeezing to the red seed squeezing of phoenix, produce the red seed oil of phoenix.
3. in four brown bottles, accurately take respectively the red seed oil of 9.9000g phoenix, stand-by.
The preparation of 4 additive mother liquors: accurately take citric acid, beta carotene, lycopene, each 0.5000g of Rosmarinus officinalis extract with assay balance, join successively respectively in the brown bottle that in step 3, four contain the red seed oil of 9.5000g phoenix, and sealing, put into 20 ℃ of water-baths concussion case lucifuges concussion 10min it is uniformly dispersed, be made into respectively mass fraction and be 5% citric acid mother liquor, 5% beta carotene mother liquor, 5% lycopene mother liquor and 5% Rosmarinus officinalis extract mother liquor.Be stored under 4 ℃ of air-proof conditions, standby.
5. add citric acid mother liquor: take out 2mL5% citric acid mother liquor, join in the red seed oil of 96mL phoenix, 20 ℃ of Water Unders are bathed concussion 20min, and it is uniformly dispersed, and obtain the mixture of the red seed oil of citric acid and phoenix, called after mixture I.
6. add beta carotene and lycopene mother liquor: take out respectively the beta carotene mother liquor of 0.6mL5% and the lycopene mother liquor of 0.8mL5%, join in the mixture I that step 5 obtains, 20 ℃ of Water Unders are bathed concussion 30min, it is uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene and phoenix, called after mixture II.
7. standing mixt II: mixture II is refrigerated to 48h under the condition of 4 ℃.
8. add Rosmarinus officinalis extract mother liquor: get respectively 0.6mL5% Rosmarinus officinalis extract mother liquor and join in the mixture II after standing, 20 ℃ of Water Unders are bathed concussion 20min, it is uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, the red seed oil of the phoenix with oxidation stability obtaining for the present invention.
Embodiment 4
The red seed oil of a kind of stability phoenix, is comprised of the material of following mass percent: 0.06% beta carotene, 0.06% lycopene, 0.07% Rosmarinus officinalis extract and 0.05% citric acid, surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. pluck the red seed of phoenix: choose the red seed of phoenix of full grains, clean the earth on the red seed of phoenix, dry under natural conditions.
2. pulverize dry: the red seed of clean phoenix is put into Chinese herbal medicine atomizer and pulverize, cross 100 mesh sieves, the dry red powder of seeds of phoenix that obtains.
3. extract the red seed oil of phoenix, adopt lixiviation process to extract the red seed oil of phoenix.
4. separating and filtering: after having extracted, the mixed liquor with the red powder of seeds of Filter paper filtering phoenix with benzinum, filter paper lower floor obtains benzinum filtrate, and filter paper upper strata obtains the phoenix pellet powder of seeds of degreasing.
5. drying under reduced pressure: adopting Rotary Evaporators is 50kPa in vacuum, and under the condition that temperature is 40 ℃, rotary evaporation also reclaims benzinum, until benzinum is distilled out of completely, obtains the red seed oil of phoenix.
6. in four brown bottles, accurately take respectively the red seed oil of 9.9000g phoenix, stand-by.
7. the preparation of additive mother liquor: accurately take citric acid, beta carotene, lycopene, each 0.1000g of Rosmarinus officinalis extract with assay balance, join successively respectively in the brown bottle that in step 6, four contain the red seed oil of 9.9000g phoenix, and sealing, put into 20 ℃ of water-baths concussion case lucifuges concussion 10min it is uniformly dispersed, be made into respectively mass fraction and be 1% citric acid mother liquor, 1% beta carotene mother liquor, 1% lycopene mother liquor and 1% Rosmarinus officinalis extract mother liquor.Be stored under 0 ℃ of air-proof condition, standby.
8. add citric acid mother liquor: take out 5mL1% citric acid mother liquor, join in the red seed oil of 76mL phoenix, 20 ℃ of Water Unders are bathed concussion 10min, and it is uniformly dispersed, and obtain the mixture of the red seed oil of citric acid and phoenix, called after mixture I.
9. add beta carotene and lycopene mother liquor: take out respectively the beta carotene mother liquor of 6mL1% and the lycopene mother liquor of 6mL1%, join in the mixture I that step 8 obtains, 15 ℃ of Water Unders are bathed concussion 20min, it is uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene and phoenix, called after mixture II.
10. standing mixt II: mixture II is refrigerated to 36h under the condition of 4 ℃.
11. add Rosmarinus officinalis extract mother liquors: get respectively 7mL1% Rosmarinus officinalis extract mother liquor and join in the mixture II after standing, 15 ℃ of Water Unders are bathed concussion 20min, it is uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, the red seed oil of the phoenix with oxidation stability obtaining for the present invention.
Embodiment 5
The red seed oil of a kind of stability phoenix, is comprised of the material of following mass percent: 0.04% beta carotene, 0.03% lycopene, 0.07% Rosmarinus officinalis extract and 0.03% citric acid, surplus is the red seed oil of phoenix.
Except addition is made corresponding change, preparation method is with embodiment 1.
Embodiment 6
The red seed oil of a kind of stability phoenix, is comprised of the material of following mass percent: 0.02% beta carotene, 0.04% lycopene, 0.05% Rosmarinus officinalis extract and 0.01% citric acid, surplus is the red seed oil of phoenix.
Except addition is made corresponding change, preparation method is with embodiment 1.
Embodiment 7
The red seed oil of a kind of stability phoenix, is comprised of the material of following mass percent: 0.06% beta carotene, 0.06% lycopene, 0.07% Rosmarinus officinalis extract and 0.05% citric acid, surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. extract the red seed oil of phoenix: adopt milling process to extract the red seed oil of phoenix.
2. four kinds of additive mother liquors of preparation: be made into respectively mass fraction and be 1% citric acid mother liquor, 1% beta carotene mother liquor, 1% lycopene mother liquor and 1% Rosmarinus officinalis extract mother liquor.Be stored under 0 ℃ of air-proof condition, standby.
3. add citric acid mother liquor: citric acid mother liquor is added in the red seed oil of phoenix, be uniformly dispersed, make the final concentration 0.05% of citric acid.
4. add beta carotene and lycopene mother liquor: beta carotene and lycopene mother liquor are added in the red seed oil of phoenix, be uniformly dispersed, making final concentration is beta carotene 0.06%, lycopene 0.06%.
5. standing refrigeration: the mixture that step 4 is obtained is standing refrigeration 48h under the condition of 0 ℃.
6. add Rosmarinus officinalis extract mother liquor: Rosmarinus officinalis extract mother liquor is added in the red seed oil of phoenix, be uniformly dispersed, make the final concentration 0.05% of Rosmarinus officinalis extract.Finally obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, the red seed oil of the phoenix with the stability finished product obtaining for the present invention.
Embodiment 8
The red seed oil of a kind of stability phoenix, is comprised of the material of following mass percent: 0.03% beta carotene, 0.02% lycopene, 0.07% Rosmarinus officinalis extract and 0.04% citric acid, surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. extract the red seed oil of phoenix: adopt No. six extraction methods to extract the red seed oil of phoenix.
2. four kinds of additive mother liquors of preparation: be made into respectively mass fraction and be 5% citric acid mother liquor, 5% beta carotene mother liquor, 5% lycopene mother liquor and 5% Rosmarinus officinalis extract mother liquor.Be stored under 0 ℃ of air-proof condition, standby.
3. add citric acid mother liquor: citric acid mother liquor is added in the red seed oil of phoenix, be uniformly dispersed, make the final concentration 0.04% of citric acid
4. add beta carotene and lycopene mother liquor: beta carotene and lycopene mother liquor are added in the red seed oil of phoenix, be uniformly dispersed, making final concentration is beta carotene 0.03%, lycopene 0.02%.
5. standing refrigeration: the mixture that step 4 is obtained is standing refrigeration 24h under the condition of 0 ℃.
6. add Rosmarinus officinalis extract mother liquor: Rosmarinus officinalis extract mother liquor is added in the red seed oil of phoenix, be uniformly dispersed, make the final concentration 0.07% of Rosmarinus officinalis extract finally obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, the red seed oil of the phoenix with the stability finished product obtaining for the present invention.
Embodiment 9
The red seed oil of a kind of stability phoenix, is comprised of the material of following mass percent: 0.03% beta carotene, 0.02% lycopene, 0.03% Rosmarinus officinalis extract and 0.03% citric acid, surplus is the red seed oil of phoenix.
A preparation method for the red seed oil of stability phoenix, as shown in Figure 1, comprises the steps:
1. extract the red seed oil of phoenix: adopt hydraulic oil press to extract the red seed oil of phoenix.
2. four kinds of additive mother liquors of preparation: be made into respectively mass fraction and be 0.5% citric acid mother liquor, 0.5% beta carotene mother liquor, 0.5% lycopene mother liquor and 0.5% Rosmarinus officinalis extract mother liquor.Be stored under 4 ℃ of air-proof conditions, standby.
3. add citric acid mother liquor: citric acid mother liquor is added in the red seed oil of phoenix, be uniformly dispersed, the final concentration that makes citric acid is 0.03%.
4. add beta carotene and lycopene mother liquor: beta carotene and lycopene mother liquor are added in the red seed oil of phoenix, be uniformly dispersed, making final concentration is beta carotene 0.03%, lycopene 0.02%.
5. standing refrigeration: the mixture that step 4 is obtained is standing refrigeration 24h under the condition of 0 ℃.
6. add Rosmarinus officinalis extract mother liquor: Rosmarinus officinalis extract mother liquor is added in the red seed oil of phoenix, be uniformly dispersed, make the final concentration 0.05% of Rosmarinus officinalis extract finally obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix, the red seed oil of the phoenix with the stability finished product obtaining for the present invention.
Claims (5)
1. the red seed of oxidation stability phoenix is oily, it is characterized in that: described in there is oxidation stability the red seed oil of phoenix comprise that mass fraction is 0.02%-0.06% beta carotene, 0.03%-0.06% lycopene, 0.03%-0.07% Rosmarinus officinalis extract and 0.01%-0.10% citric acid, surplus is the red seed oil of phoenix.
2. the red seed of oxidation stability phoenix according to claim 1 is oily, it is characterized in that: described in there is oxidation stability the red seed oil of phoenix by the material of following mass percent, formed: 0.02% beta carotene, 0.03% lycopene, 0.04% Rosmarinus officinalis extract and 0.02% citric acid, surplus is the red seed oil of phoenix.
3. the preparation method of the red seed oil of oxidation stability phoenix claimed in claim 1, is characterized in that: described preparation method comprises step successively: with the red seed oil of phoenix preparation citric acid, beta carotene, lycopene and Rosmarinus officinalis extract mother liquor; Add citric acid mother liquor; Add beta carotene and lycopene mother liquor; The standing mixture 24-48h that contains beta carotene, lycopene and citric acid; Add Rosmarinus officinalis extract mother liquor.
4. the preparation method of the red seed oil of oxidation stability phoenix according to claim 3, is characterized in that: described preparation method comprises step:
(1) extract the red seed oil of phoenix: adopt lixiviation process to extract the grease in the red seed of phoenix, obtain the red seed oil of phoenix;
(2) prepare four kinds of additive mother liquors: the citric acid mother liquor, the beta carotene mother liquor of 0.5%-5%, the Rosmarinus officinalis extract mother liquor of the lycopene mother liquor of 0.5%-5% and 0.5%-5% that with the red seed oil of phoenix preparation mass fraction, are 0.5%-5% respectively;
(3) add citric acid mother liquor: take out citric acid mother liquor, join in the red seed oil of phoenix, be uniformly dispersed, obtain mixture I;
(4) add beta carotene and lycopene mother liquor: take out respectively beta carotene mother liquor and lycopene mother liquor, join in mixture I, be uniformly dispersed, obtain mixture II;
(5) standing refrigeration: by mixture II refrigeration 24-48h;
(6) add Rosmarinus officinalis extract mother liquor: get Rosmarinus officinalis extract mother liquor and join in the mixture II after standing refrigeration, be uniformly dispersed, obtain the mixture of the red seed oil of citric acid, beta carotene, lycopene, Rosmarinus officinalis extract and phoenix.
5. the preparation method of the red seed oil of oxidation stability phoenix according to claim 4, is characterized in that: described employing lixiviation process extracts the grease in the red seed of phoenix or is milling process.
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Cited By (6)
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| CN104543046A (en) * | 2015-02-02 | 2015-04-29 | 吉首大学 | Giant salamander oil natural composite antioxidant |
| CN105219519A (en) * | 2015-09-17 | 2016-01-06 | 韦木兰 | A kind of preparation method of flower of Greenish Lily fragrant vegetable seed oil |
| CN106720464A (en) * | 2016-11-24 | 2017-05-31 | 菏泽尧舜牡丹生物科技有限公司 | It is a kind of to be adapted to peony seeds flavored oils designed for old people |
| CN106943487A (en) * | 2017-03-31 | 2017-07-14 | 刘海龙 | A kind of composition containing rosemary, preparation method and applications |
| CN111642675A (en) * | 2020-06-19 | 2020-09-11 | 海南舒普生物科技有限公司 | Natural antioxidant color-protecting and flavor-protecting compound additive and preparation method thereof |
| CN114343062A (en) * | 2021-12-20 | 2022-04-15 | 广州合诚三先生物科技有限公司 | Antioxidant composition and preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104543046A (en) * | 2015-02-02 | 2015-04-29 | 吉首大学 | Giant salamander oil natural composite antioxidant |
| CN105219519A (en) * | 2015-09-17 | 2016-01-06 | 韦木兰 | A kind of preparation method of flower of Greenish Lily fragrant vegetable seed oil |
| CN106720464A (en) * | 2016-11-24 | 2017-05-31 | 菏泽尧舜牡丹生物科技有限公司 | It is a kind of to be adapted to peony seeds flavored oils designed for old people |
| CN106943487A (en) * | 2017-03-31 | 2017-07-14 | 刘海龙 | A kind of composition containing rosemary, preparation method and applications |
| CN111642675A (en) * | 2020-06-19 | 2020-09-11 | 海南舒普生物科技有限公司 | Natural antioxidant color-protecting and flavor-protecting compound additive and preparation method thereof |
| CN111642675B (en) * | 2020-06-19 | 2023-01-31 | 海南舒普生物科技有限公司 | Natural antioxidant color-protecting and flavor-protecting compound additive and preparation method thereof |
| CN114343062A (en) * | 2021-12-20 | 2022-04-15 | 广州合诚三先生物科技有限公司 | Antioxidant composition and preparation method and application thereof |
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