CN103601813A - Extraction method for ganoderma lucidum polysaccharide - Google Patents
Extraction method for ganoderma lucidum polysaccharide Download PDFInfo
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- CN103601813A CN103601813A CN201310650365.4A CN201310650365A CN103601813A CN 103601813 A CN103601813 A CN 103601813A CN 201310650365 A CN201310650365 A CN 201310650365A CN 103601813 A CN103601813 A CN 103601813A
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- ganoderan
- ganoderma lucidum
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Abstract
Belonging to the technical field of natural polymers, the invention discloses an extraction method for ganoderma lucidum polysaccharide. The extraction method for ganoderma lucidum polysaccharide comprises the steps of: removing fat from ganoderma lucidum fruit body dry powder, adding the ganoderma lucidum fruit body dry powder into an extracting agent composed of water and glycerin to perform extraction, then carrying out filtration, and collecting the filtrate; re-extracting the filter residue by the extracting agent several times, carrying out filtration, and collecting the filtrate; combining the filtrate, and dissolving ganoderma lucidum polysaccharide in the obtained filtrate; then further performing ultrafiltration concentration to obtain a ganoderma lucidum polysaccharide solution; or conducting ultrafiltration to remove part of glycerin or water, thus obtaining a glycerin-containing ganoderma lucidum polysaccharide water solution. The glycerin-containing ganoderma lucidum polysaccharide water solution can be used as a functional additive of cosmetics or masks. The method provided by the invention makes use of the hydroxyl in the glycerin and polysaccharide to form strong hydrogen-bond interaction, is in favor of dissolution of polysaccharide, and enhances the extraction efficiency. Also the used extracting agent is safe, and environmental pollution caused by chloroform, methanol and other organic solvents or acid-base employed by traditional methods can be avoided.
Description
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Technical field
The invention belongs to natural polymer technical field, relate to a kind of extracting method of ganoderan.
Background technology
Ganoderan is a kind of a kind of natural high moleculer eompound extracting from Ganoderma spore powder or Ganoderma sporophore, and its molecular weight is from thousands of to hundreds thousand of.The effects such as that ganoderan has is antitumor, strengthening immunity, moisturizing.
Traditional ganoderan extracting method is mainly to adopt hot water extraction, acidleach formulation, alkali method etc.Though acid or alkali method can improve extraction yield, high and very easily destroy the structure of polysaccharide to equipment requirements, also there is problem of environmental pollution.Traditional hot water extraction time is grown, consumes energy high, and active component extract yield is low.
Summary of the invention
The shortcoming that the object of the invention is to overcome prior art, with not enough, provides a kind of safety and environmental protection to extract efficiently the method for ganoderan.
Object of the present invention is achieved through the following technical solutions:
An extracting method for ganoderan, comprises the steps: Ganoderma sporophore dry powder to remove after fat, joins in extraction agent and extracts, and filters, and collects filtrate; Extraction agent extracted several times again for filter residue, filters, and collects filtrate; Merge above-mentioned filtrate, ganoderan is dissolved in filtrate; Described extraction agent is the mixed solvent of water and glycerine.
In described extraction agent, the mass ratio of water and glycerine is preferably 5～1000:1.
The quality optimization of described extraction agent is 1～20 times of Ganoderma sporophore dry powder.
Described extraction is preferably stirs extraction or ultrasonic extraction, and the frequency of described ultrasonic extraction is 20～100KHz; Preferred, described extraction is at 20～130 ℃, to stir extraction or ultrasonic extraction 4～48 h.
Described several is preferably 0～2 time.
Preferably, the extracting method of described ganoderan, also comprises the steps: the filtrate of merging to obtain ganoderan solution by ultrafiltration and concentration; Or remove partial glycerol or water by ultrafiltration, obtain the ganoderan aqueous solution that contains glycerine.
By aforesaid method, extract the ganoderan aqueous solution that contains glycerine obtaining, can be as the functional additive of makeup or facial mask.Also can take as required hydrogen peroxide decolouring, deviate from the methods such as protein ganoderan is carried out to purifying.
The present invention has the following advantages and effect with respect to prior art tool: the present invention's extraction agent used is the mixed solvent of water and glycerine, utilize hydroxyl and polysaccharide in glycerine to form strong hydrogen bond action, be conducive to the stripping of polysaccharide, improve extraction efficiency, and solvent for use safety, has avoided adopting in traditional method the pollution of the organic solvents such as chloroform, methyl alcohol or acid base pair environment.
Embodiment
Below in conjunction with embodiment, the present invention is done to further detailed description, but embodiments of the present invention are not limited to this.
Embodiment 1
(1) get the dry Ganoderma sporophore that 50.0 g crush, adopt to be extracted with ethyl acetate and remove after fat, pack in ultrasonic extraction still, the mixed solvent (mass ratio of water and glycerine is 5:1) that adds 50 g water and glycerine, under 130 ℃ and 50 KHz ultrasonications, extract 15 h, drop to room temperature, filter, collect filtrate; Filter residue is continued to add above-mentioned mixed solvent, extracting twice repeatedly under similarity condition; 3 gained filtrates are merged.
(2), by above-mentioned filtrate ultrafiltration and concentration, obtain ganoderan mass content and be 9.19% aqueous glycerin solution 20 g.
Embodiment 2
(1) get the dry Ganoderma sporophore that 50.0 g crush, adopt to be extracted with ethyl acetate and remove after fat, pack in ultrasonic extraction still, the mixed solvent (mass ratio of water and glycerine is 1000:1) that adds 1000 g water and glycerine, under 100 ℃ and 20 KHz ultrasonications, extract 48 h, drop to room temperature, filter, collect filtrate.
(2), by above-mentioned filtrate ultrafiltration and concentration, obtain ganoderan mass content and be 6.77% aqueous glycerin solution 20 g.
Embodiment 3
(1) get the dry Ganoderma sporophore that 50.0 g crush, adopt to be extracted with ethyl acetate and remove after fat, pack in extraction kettle, the mixed solvent (mass ratio of water and glycerine is 9:1) that adds 500 g water and glycerine, at 20 ℃, stir extraction 48 h, filter, collect filtrate; Filter residue is continued to add above-mentioned mixed solvent, extracting twice repeatedly under similarity condition; 3 gained filtrates are merged.
(2), by above-mentioned filtrate ultrafiltration and concentration, obtain ganoderan mass content and be 11.3% aqueous glycerin solution 20 g.
Embodiment 4
(1) get the dry Ganoderma sporophore that 50.0 g crush, adopt to be extracted with ethyl acetate and remove after fat, pack in ultrasonic extraction still, the mixed solvent (mass ratio of water and glycerine is 6:1) that adds 210 g water and glycerine, under 100 ℃ and 100 KHz ultrasonications, extract 4 h, drop to room temperature, filter, collect filtrate; Filter residue is continued to add above-mentioned mixed solvent, extracting twice repeatedly under similarity condition; 3 gained filtrates are merged.
(2), by above-mentioned filtrate ultrafiltration and concentration, obtain ganoderan mass content and be 11.7% aqueous glycerin solution 20 g.
Embodiment 5
(1) get the dry Ganoderma sporophore that 50.0 g crush, adopt to be extracted with ethyl acetate and remove after fat, pack in ultrasonic extraction still, the mixed solvent (mass ratio of water and glycerine is 5:1) that adds 300 g water and glycerine, under 100 ℃ and 50 KHz ultrasonications, extract 24 h, drop to room temperature, filter, collect filtrate; Filter residue is continued to add above-mentioned mixed solvent, extracting twice repeatedly under similarity condition; 3 gained filtrates are merged.
(2) above-mentioned filtrate is used to ultrafiltration circular treatment, pass into continuously glycerine and filtrate, the water with in glycerine displacement filtrate, makes the concentration of glycerine reach 70%, then with ultrafiltration, concentrates, and obtains ganoderan mass content and be 12.7% aqueous glycerin solution 20 g.
Embodiment 6
(1) get the dry Ganoderma sporophore that 50.0 g crush, adopt to be extracted with ethyl acetate and remove after fat, pack in ultrasonic extraction still, the mixed solvent (mass ratio of water and glycerine is 3:1) that adds 200 g water and glycerine, under 100 ℃ and 50 KHz ultrasonications, extract 24 h, drop to room temperature, filter, collect filtrate; Filter residue is continued to add above-mentioned mixed solvent, extracting twice repeatedly under similarity condition; 3 gained filtrates are merged.
(2) above-mentioned filtrate is used to ultrafiltration circular treatment, pass into continuously glycerine and filtrate, the glycerine in water displacement filtrate, makes the mass concentration of glycerine lower than 0.1%, then with ultrafiltration, concentrates, and obtains ganoderan mass content and be 10.1% aqueous glycerin solution 20 g.
Above-described is only some embodiments of the present invention, but embodiments of the present invention are not restricted to the described embodiments.For the person of ordinary skill of the art, other any do not deviate from change, the modification done under spirit of the present invention and principle, substitutes, combination, simplify, and all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (9)
1. an extracting method for ganoderan, is characterized in that comprising the steps: Ganoderma sporophore dry powder is removed after fat, joins in extraction agent and extracts, and filters, and collects filtrate; Extraction agent extracted several times again for filter residue, filters, and collects filtrate; Merge above-mentioned filtrate, ganoderan is dissolved in filtrate; Described extraction agent is the mixed solvent of water and glycerine.
2. the extracting method of ganoderan according to claim 1, is characterized in that: in described extraction agent, the mass ratio of water and glycerine is 5～1000:1.
3. the extracting method of ganoderan according to claim 1, is characterized in that: the quality of described extraction agent is 1～20 times of Ganoderma sporophore dry powder.
4. the extracting method of ganoderan according to claim 1, is characterized in that: described extraction is for stirring extraction or ultrasonic extraction, and the frequency of described ultrasonic extraction is 20～100KHz.
5. the extracting method of ganoderan according to claim 4, is characterized in that: described extraction for stirring extraction or ultrasonic extraction 4～48 h at 20～130 ℃.
6. the extracting method of ganoderan according to claim 1, is characterized in that: described several is 0～2 time.
7. according to the extracting method of the ganoderan described in claim 1-6 any one, characterized by further comprising following steps: the filtrate of merging is obtained to ganoderan solution by ultrafiltration and concentration; Or remove partial glycerol or water by ultrafiltration, obtain the ganoderan aqueous solution that contains glycerine.
8. a ganoderan aqueous solution that contains glycerine, is characterized in that: by extracting method claimed in claim 7, obtain.
9. the application of the ganoderan aqueous solution that contains glycerine claimed in claim 8 in the functional additive of makeup or facial mask.
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| CN201310650365.4A CN103601813A (en) | 2013-12-06 | 2013-12-06 | Extraction method for ganoderma lucidum polysaccharide |
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| CN201310650365.4A CN103601813A (en) | 2013-12-06 | 2013-12-06 | Extraction method for ganoderma lucidum polysaccharide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106117386A (en) * | 2016-06-30 | 2016-11-16 | 卢月莲 | The method that molecular engram extracts ganoderan with ultrasonic coupling |
| CN109223607A (en) * | 2018-11-27 | 2019-01-18 | 权能(深圳)生物科技有限公司 | A kind of crease-resistant moisturizing cosmetic composition and cosmetics |
| CN110467686A (en) * | 2019-09-17 | 2019-11-19 | 齐鲁工业大学 | A kind of highly effective extraction method of Root of Asparagus officinalis polysaccharide |
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| CN101704903A (en) * | 2009-11-18 | 2010-05-12 | 中国药科大学 | Preparation method of low molecular glossy ganoderma polysaccharide |
| CN102093486A (en) * | 2011-01-04 | 2011-06-15 | 广东省微生物研究所 | Decolorized ganoderma lucidum polysaccharide extract and method for preparing hand cream by using same |
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| CN1613325A (en) * | 2004-09-20 | 2005-05-11 | 南开大学 | Natural lemon antiseptics and preparation thereof |
| CN101980695B (en) * | 2008-05-16 | 2013-05-22 | 株式会社日冷生物科学 | Extract produced from orchid family plant, method for production thereof, and preparation for external application to skin comprising extract produced from orchid family plant |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106117386A (en) * | 2016-06-30 | 2016-11-16 | 卢月莲 | The method that molecular engram extracts ganoderan with ultrasonic coupling |
| CN109223607A (en) * | 2018-11-27 | 2019-01-18 | 权能(深圳)生物科技有限公司 | A kind of crease-resistant moisturizing cosmetic composition and cosmetics |
| CN109223607B (en) * | 2018-11-27 | 2021-06-01 | 权能(深圳)生物科技有限公司 | Anti-wrinkle moisturizing skin-brightening composition and cosmetic |
| CN110467686A (en) * | 2019-09-17 | 2019-11-19 | 齐鲁工业大学 | A kind of highly effective extraction method of Root of Asparagus officinalis polysaccharide |
| CN110467686B (en) * | 2019-09-17 | 2021-03-30 | 齐鲁工业大学 | Efficient extraction method of asparagus root polysaccharide |
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Application publication date: 20140226 |