CN103585628A - Immunoglobulin preparation for treating newcastle disease and preparation method thereof - Google Patents
Immunoglobulin preparation for treating newcastle disease and preparation method thereof Download PDFInfo
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- CN103585628A CN103585628A CN201310587096.1A CN201310587096A CN103585628A CN 103585628 A CN103585628 A CN 103585628A CN 201310587096 A CN201310587096 A CN 201310587096A CN 103585628 A CN103585628 A CN 103585628A
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- newcastle disease
- citric acid
- immunoglobulin preparation
- ice ethanol
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Abstract
The invention relates to a targeted treatment drug for treating the newcastle disease, in particular to an immunoglobulin preparation for treating the newcastle disease and a preparation method thereof. The immunoglobulin preparation comprises the following main components by volume percent: 0.02%-0.1% of a citric acid solution, 10%-30% of absolute alcohol, 0.2%-0.8% of trypsin and 0.5%-1% of normal saline. The immunoglobulin preparation has the benefits that the immunoglobulin preparation can be used for oral administration after extracted globulins are hydrolyzed and coated, so that the operability of the immunoglobulin preparation used in the modern chicken-raising industry is greatly improved, the problems of depressed mood, low feed intake and prolonged slaughtering time of broiler chickens and reduced laying rate of egg chickens after the newcastle disease happens are effectively solved, the economic loss due to mass mortality of the chickens caused by the newcastle disease is avoided, and the blank of the industry is filled.
Description
Technical field
The present invention relates to a kind of immunotherapy targeted autoantibody medicine for the treatment of newcastle disease, particularly a kind of immunoglobulin preparation for the treatment of Newcastle disease and preparation method thereof.
Background technology
China's poultry husbandry development rapidly, the cultivation amount whole world the first, but disease puzzlement in cultivation is also a lot, especially the newcastle of chicken (being commonly called as fowl plague), is comparatively common disease, and this disease only has vaccine to prevent now, morbidity is not directly used in the medicine for the treatment of, once can cause larger loss, broiler spirit is depressed, feed intake is low, causes marketing time to extend, and laying hen is caused egg drop reduction, there is large quantities of death in severe patient, causes larger economic loss.
Summary of the invention
The present invention is in order to make up the defect of prior art, provide a kind of take the quick control state of an illness, reduce immunoglobulin preparation and preparation method thereof that cultivation loss is the treatment Newcastle disease on order ground.
Immunoglobulin preparation for the treatment of Newcastle disease of the present invention and preparation method thereof, its key component volume ratio is: citric acid solution 0.02%-0.1%, ice ethanol 10%-30%, trypsin 0.2%-0.8%, normal saline 0.5%-1%.
Preparation method of the present invention is as follows:
1) citric acid is diluted to the citric acid solution of 0.02%-0.1% and Sanguis Gallus domesticus is carried out to anticoagulant processing, the volume ratio of citric acid solution and Sanguis Gallus domesticus is 2%-8%;
2) the fresh Sanguis Gallus domesticus that anticoagulant was processed through citric acid solution is obtained to serum by low speed refrigerated centrifuge centrifugalize;
3) centrifugalize is obtained to serum add the ice ethanol of-20 ℃, the volume ratio of ice ethanol and serum is 10%-30%;
4) will add the serum of-20 ℃ of ice ethanol by low speed refrigerated centrifuge centrifuging and taking precipitate;
5) precipitate obtaining by low speed refrigerated centrifuge centrifuging and taking is dissolved in containing stirring 0.5-1.5 hour in the tryptic normal saline of 0.2%-0.8%, precipitate is 0.5%-1% with the volume ratio containing the tryptic normal saline of 0.2%-0.8%;
6) solution after stirring is added to the ice ethanol of-20 ℃, the volume ratio of solution and ice ethanol is 10%-30%;
7) the centrifuge centrifuging and taking that is 2 ℃-6 ℃ by the ice alcoholic solution that adds-20 ℃ by temperature precipitates;
8) beta-cyclodextrin inclusion compound;
9) spraying is dry; Intake air temperature is 150 ℃-170 ℃, and air outlet temperature is 60 ℃-85 ℃, atomizing disk rotating speed 17500-18500 rev/min;
10) receive powder packing.
Immunoglobulin preparation for the treatment of Newcastle disease of the present invention and preparation method thereof, adopting fresh Sanguis Gallus domesticus is raw material, with cold ethanol method, from Sanguis Gallus domesticus, extract immunoglobulin, purified, hydrolysis, make after coated and can supply oral preparation, be used for the treatment of the Newcastle Disease of chicken.
The invention has the beneficial effects as follows: this product can be used as orally using after the globulin extracting is hydrolyzed, is coated with, greatly improved the operability of product in modern poultry husbandry use procedure, there is the phenomenon that rear broiler spirit is depressed, feed intake low, marketing time extends and Egg Production of Laying Hens declines in the newcastle disease that has effectively solved chicken, the economic loss of avoiding the newcastle disease because of chicken to cause the large quantities of death of chicken to bring, has filled up industry blank.
Case is given an example:
1, Jinan plant of Huaxing, 2000 of broiler, 3600 of laying hens,, there is newcastle disease disease in hydrolysis oral medication, wherein broiler is dead 48 after 20 days, dead 23 of laying hen, all are normal for other index of remaining broiler and layer feed intake, laying hen egg yield and broiler.
2, plumage poultry farms in Henan, 5300 of broiler, 2200 of laying hens,, there is newcastle disease disease in hydrolysis oral medication, wherein broiler is dead 63 after 28 days, dead 35 of laying hen, all are normal for other index of remaining broiler and layer feed intake, laying hen egg yield and broiler.
3, the emerging brutish cultivation of Jiangsu water center, 11200 of broiler, 24600 of laying hens, hydrolysis oral medication, after 65 days, there is newcastle disease disease, wherein broiler is dead 152, dead 191 of laying hen, and remaining broiler and layer is the phenomenon that occurs that spirit is depressed, feed intake low, marketing time extends and Egg Production of Laying Hens declines.
The specific embodiment
Below provide specific embodiments of the invention, be used for formation of the present invention to be further described.
Embodiment 1
Immunoglobulin preparation for the treatment of Newcastle disease of the present invention and preparation method thereof, its key component volume ratio is: citric acid solution 0.02%, ice ethanol 10%-30%, trypsin 0.2%, normal saline 0.5%.
Preparation method of the present invention is as follows:
1) citric acid is diluted to 0.02% citric acid solution and Sanguis Gallus domesticus is carried out to anticoagulant processing, the volume ratio of citric acid solution and Sanguis Gallus domesticus is 2%;
2) the fresh Sanguis Gallus domesticus that anticoagulant was processed through citric acid solution is obtained to serum by low speed refrigerated centrifuge centrifugalize;
3) centrifugalize is obtained to serum add the ice ethanol of-20 ℃, the volume ratio of ice ethanol and serum is 10%;
4) will add the serum of-20 ℃ of ice ethanol by low speed refrigerated centrifuge centrifuging and taking precipitate;
5) precipitate obtaining by low speed refrigerated centrifuge centrifuging and taking is dissolved in containing in 0.2% tryptic normal saline and stirs 0.5 hour, precipitate is 0.5% with the volume ratio containing 0.2% tryptic normal saline;
6) solution after stirring is added to the ice ethanol of-20 ℃, the volume ratio of solution and ice ethanol is 30%;
7) the centrifuge centrifuging and taking that is 2 ℃ by the ice alcoholic solution that adds-20 ℃ by temperature precipitates;
8) beta-cyclodextrin inclusion compound;
9) spraying is dry; Intake air temperature is 150 ℃, and air outlet temperature is 60 ℃, 17500 revs/min of atomizing disk rotating speeds;
10) receive powder packing.
Embodiment 2
Immunoglobulin preparation for the treatment of Newcastle disease of the present invention and preparation method thereof, its key component volume ratio is: citric acid solution 0.04%, ice ethanol 10%-30%, trypsin 0.3%, normal saline 0.7%.
Preparation method of the present invention is as follows:
1) citric acid is diluted to 0.04% citric acid solution and Sanguis Gallus domesticus is carried out to anticoagulant processing, the volume ratio of citric acid solution and Sanguis Gallus domesticus is 3%;
2) the fresh Sanguis Gallus domesticus that anticoagulant was processed through citric acid solution is obtained to serum by low speed refrigerated centrifuge centrifugalize;
3) centrifugalize is obtained to serum add the ice ethanol of-20 ℃, the volume ratio of ice ethanol and serum is 20%;
4) will add the serum of-20 ℃ of ice ethanol by low speed refrigerated centrifuge centrifuging and taking precipitate;
5) precipitate obtaining by low speed refrigerated centrifuge centrifuging and taking is dissolved in containing in 0.3% tryptic normal saline and stirs 0.8 hour, precipitate is 0.7% with the volume ratio containing 0.3% tryptic normal saline;
6) solution after stirring is added to the ice ethanol of-20 ℃, the volume ratio of solution and ice ethanol is 18%;
7) the centrifuge centrifuging and taking that is 3 ℃ by the ice alcoholic solution that adds-20 ℃ by temperature precipitates;
8) beta-cyclodextrin inclusion compound;
9) spraying is dry; Intake air temperature is 160 ℃, and air outlet temperature is 65 ℃, 18000 revs/min of atomizing disk rotating speeds;
10) receive powder packing.
Embodiment 3
Immunoglobulin preparation for the treatment of Newcastle disease of the present invention and preparation method thereof, its key component volume ratio is: citric acid solution 0.08%, ice ethanol 10%-30%, trypsin 0.5%, normal saline 0.9%.
Preparation method of the present invention is as follows:
1) citric acid is diluted to 0.08% citric acid solution and Sanguis Gallus domesticus is carried out to anticoagulant processing, the volume ratio of citric acid solution and Sanguis Gallus domesticus is 4%;
2) the fresh Sanguis Gallus domesticus that anticoagulant was processed through citric acid solution is obtained to serum by low speed refrigerated centrifuge centrifugalize;
3) centrifugalize is obtained to serum add the ice ethanol of-20 ℃, the volume ratio of ice ethanol and serum is 25%;
4) will add the serum of-20 ℃ of ice ethanol by low speed refrigerated centrifuge centrifuging and taking precipitate;
5) precipitate obtaining by low speed refrigerated centrifuge centrifuging and taking is dissolved in containing in 0.5% tryptic normal saline and stirs 1 hour, precipitate is 0.9% with the volume ratio containing 0.5% tryptic normal saline;
6) solution after stirring is added to the ice ethanol of-20 ℃, the volume ratio of solution and ice ethanol is 15%;
7) the centrifuge centrifuging and taking that is 4 ℃ by the ice alcoholic solution that adds-20 ℃ by temperature precipitates;
8) beta-cyclodextrin inclusion compound;
9) spraying is dry; Intake air temperature is 165 ℃, and air outlet temperature is 75 ℃, 18200 revs/min of atomizing disk rotating speeds;
10) receive powder packing.
Embodiment 4
Immunoglobulin preparation for the treatment of Newcastle disease of the present invention and preparation method thereof, its key component volume ratio is: citric acid solution 0.1%, ice ethanol 10%-30%, trypsin 0.8%, normal saline 1%.
Preparation method of the present invention is as follows:
1) citric acid is diluted to 0.1% citric acid solution and Sanguis Gallus domesticus is carried out to anticoagulant processing, the volume ratio of citric acid solution and Sanguis Gallus domesticus is 8%;
2) the fresh Sanguis Gallus domesticus that anticoagulant was processed through citric acid solution is obtained to serum by low speed refrigerated centrifuge centrifugalize;
3) centrifugalize is obtained to serum add the ice ethanol of-20 ℃, the volume ratio of ice ethanol and serum is 30%;
4) will add the serum of-20 ℃ of ice ethanol by low speed refrigerated centrifuge centrifuging and taking precipitate;
5) precipitate obtaining by low speed refrigerated centrifuge centrifuging and taking is dissolved in containing in 0.8% tryptic normal saline and stirs 1.5 hours, precipitate is 1% with the volume ratio containing 0.8% tryptic normal saline;
6) solution after stirring is added to the ice ethanol of-20 ℃, the volume ratio of solution and ice ethanol is 10%;
7) the centrifuge centrifuging and taking that is 6 ℃ by the ice alcoholic solution that adds-20 ℃ by temperature precipitates;
8) beta-cyclodextrin inclusion compound;
9) spraying is dry; Intake air temperature is 170 ℃, and air outlet temperature is 85 ℃, 18500 revs/min of atomizing disk rotating speeds;
10) receive powder packing.
Claims (8)
1. treat immunoglobulin preparation of Newcastle disease and preparation method thereof for one kind, it is characterized in that: its key component has: citric acid solution, ice ethanol, trypsin, normal saline; Preparation method of the present invention is as follows:
1) citric acid is diluted to citric acid solution and Sanguis Gallus domesticus is carried out to anticoagulant processing;
2) the fresh Sanguis Gallus domesticus that anticoagulant was processed through citric acid solution is obtained to serum by low speed refrigerated centrifuge centrifugalize;
3) centrifugalize is obtained to the ice ethanol that serum adds;
4) will add the serum of ice ethanol by low speed refrigerated centrifuge centrifuging and taking precipitate;
5) precipitate obtaining by low speed refrigerated centrifuge centrifuging and taking is dissolved in containing in tryptic normal saline and stirs;
6) solution after stirring is added to ice ethanol;
7) will add ice alcoholic solution to precipitate by centrifuge centrifuging and taking;
8) beta-cyclodextrin inclusion compound;
9) spraying is dry;
10) receive powder packing.
2. a kind of immunoglobulin preparation for the treatment of Newcastle disease according to claim 1 and preparation method thereof, it is characterized in that: described its key component volume ratio is: citric acid solution 0.02%-0.1%, ice ethanol 10%-30%, trypsin 0.2%-0.8%, normal saline 0.5%-1%.
3. a kind of immunoglobulin preparation for the treatment of Newcastle disease according to claim 1 and preparation method thereof, it is characterized in that: described citric acid is diluted to the citric acid solution of 0.02%-0.1% and Sanguis Gallus domesticus is carried out to anticoagulant processing, the volume ratio of citric acid solution and Sanguis Gallus domesticus is 2%-8%.
4. a kind of immunoglobulin preparation for the treatment of Newcastle disease according to claim 1 and preparation method thereof, is characterized in that: describedly centrifugalize is obtained to serum and adds the ice ethanol of-20 ℃, the volume ratio of ice ethanol and serum is 10%-30%.
5. a kind of immunoglobulin preparation for the treatment of Newcastle disease according to claim 1 and preparation method thereof, it is characterized in that: described the precipitate obtaining by low speed refrigerated centrifuge centrifuging and taking is dissolved in containing stirring 0.5-1.5 hour in the tryptic normal saline of 0.2%-0.8%, precipitate is 0.5%-1% with the volume ratio containing the tryptic normal saline of 0.2%-0.8%.
6. a kind of immunoglobulin preparation for the treatment of Newcastle disease according to claim 1 and preparation method thereof, is characterized in that: describedly solution after stirring is added to the ice ethanol of-20 ℃, the volume ratio of solution and ice ethanol is 10%-30%.
7. a kind of immunoglobulin preparation for the treatment of Newcastle disease according to claim 1 and preparation method thereof, is characterized in that: the described centrifuge centrifuging and taking that is 2 ℃-6 ℃ by temperature by the ice alcoholic solution that adds-20 ℃ precipitates.
8. a kind of immunoglobulin preparation for the treatment of Newcastle disease according to claim 1 and preparation method thereof, it is characterized in that: when described spraying is dried, intake air temperature is 150 ℃-170 ℃, and air outlet temperature is 60 ℃-85 ℃, atomizing disk rotating speed 17500-18500 rev/min.
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| CN201310587096.1A CN103585628B (en) | 2013-11-21 | 2013-11-21 | A kind of immunoglobulin preparation preventing Newcastle disease and preparation method thereof |
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| CN201310587096.1A CN103585628B (en) | 2013-11-21 | 2013-11-21 | A kind of immunoglobulin preparation preventing Newcastle disease and preparation method thereof |
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| CN103585628A true CN103585628A (en) | 2014-02-19 |
| CN103585628B CN103585628B (en) | 2016-08-17 |
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Citations (7)
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| US6913751B2 (en) * | 1992-06-12 | 2005-07-05 | Schering-Plough Veterinary Corporation | Recombinant avian herpesvirus useful in vaccine production |
| CN102108096A (en) * | 2009-12-25 | 2011-06-29 | 青岛康地恩药业有限公司 | Escherichia coli recombinant chicken alpha-gamma divalent interferon and preparation method thereof |
| WO2012104647A1 (en) * | 2011-02-04 | 2012-08-09 | Anhydro Biologicals Limited | Vaccine compositions |
| CN102639148A (en) * | 2009-12-01 | 2012-08-15 | 英特维特国际股份有限公司 | Inactivated poultry vaccine |
| CN103304663A (en) * | 2012-07-03 | 2013-09-18 | 上海交通大学 | Single-chain antibody of chicken Newcastle disease virus P protein, preparation method and use of single-chain antibody |
| CN103342749A (en) * | 2013-07-29 | 2013-10-09 | 中国科学院微生物研究所 | Triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus |
| CN103341038A (en) * | 2013-07-22 | 2013-10-09 | 王兰美 | Traditional Chinese medicine prescription for treating Newcastle disease and avian influenza |
-
2013
- 2013-11-21 CN CN201310587096.1A patent/CN103585628B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6913751B2 (en) * | 1992-06-12 | 2005-07-05 | Schering-Plough Veterinary Corporation | Recombinant avian herpesvirus useful in vaccine production |
| CN102639148A (en) * | 2009-12-01 | 2012-08-15 | 英特维特国际股份有限公司 | Inactivated poultry vaccine |
| CN102108096A (en) * | 2009-12-25 | 2011-06-29 | 青岛康地恩药业有限公司 | Escherichia coli recombinant chicken alpha-gamma divalent interferon and preparation method thereof |
| WO2012104647A1 (en) * | 2011-02-04 | 2012-08-09 | Anhydro Biologicals Limited | Vaccine compositions |
| CN103304663A (en) * | 2012-07-03 | 2013-09-18 | 上海交通大学 | Single-chain antibody of chicken Newcastle disease virus P protein, preparation method and use of single-chain antibody |
| CN103341038A (en) * | 2013-07-22 | 2013-10-09 | 王兰美 | Traditional Chinese medicine prescription for treating Newcastle disease and avian influenza |
| CN103342749A (en) * | 2013-07-29 | 2013-10-09 | 中国科学院微生物研究所 | Triple egg yolk antibody against newcastle disease virus, avian influenza virus and infectious bronchitis virus |
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| CN103585628B (en) | 2016-08-17 |
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| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: The invention relates to an immunoglobulin preparation for preventing chicken Newcastle disease and a preparation method thereof Effective date of registration: 20211013 Granted publication date: 20160817 Pledgee: Qingdao Jinling Shengtai Financing Guarantee Co.,Ltd. Pledgor: QINGDAO RUNDA BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021370010100 |
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Date of cancellation: 20221208 Granted publication date: 20160817 Pledgee: Qingdao Jinling Shengtai Financing Guarantee Co.,Ltd. Pledgor: QINGDAO RUNDA BIOTECHNOLOGY Co.,Ltd. Registration number: Y2021370010100 |
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Denomination of invention: An immunoglobulin preparation for preventing Newcastle disease in chickens and its preparation method Effective date of registration: 20221212 Granted publication date: 20160817 Pledgee: Qingdao Jinling Shengtai Financing Guarantee Co.,Ltd. Pledgor: QINGDAO RUNDA BIOTECHNOLOGY Co.,Ltd. Registration number: Y2022370010165 |
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