CN103572047B - A kind of bacterium agitation leaching method of uranium ore - Google Patents
A kind of bacterium agitation leaching method of uranium ore Download PDFInfo
- Publication number
- CN103572047B CN103572047B CN201310579403.1A CN201310579403A CN103572047B CN 103572047 B CN103572047 B CN 103572047B CN 201310579403 A CN201310579403 A CN 201310579403A CN 103572047 B CN103572047 B CN 103572047B
- Authority
- CN
- China
- Prior art keywords
- leaching
- uranium
- nutrient solution
- mixed solution
- value
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Manufacture And Refinement Of Metals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A kind of bacterium agitation leaching method of uranium ore, belong to biological metallurgy technical field, carry out according to the following steps: the mixed bacteria liquid containing the micro-spirillum of iron protoxide, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans is inoculated in substratum by (1), makes nutrient solution; (2) adjust ph; Pass into air and stir and cultivate, make and leach bacterium liquid; (3) add water uranium ore stone flour furnishing ore pulp, leaching in bacterium liquid, making leaching mixed solution with joining after sulfuric acid adjust pH; (4) pH value leaching mixed solution is regulated with sulfuric acid; Pass into air and stir and leach.Method of the present invention can significantly improve the leaching yield of uranium, and reduces acid consumption; By improving leaching ore deposit process kinetics, shortening leaching cycle, improving leaching yield.Method of the present invention has that production cost is low, less investment, equipment are simple, advantages of environment protection.
Description
Technical field
The invention belongs to biological metallurgy technical field, particularly a kind of bacterium agitation leaching method of uranium ore.
Background technology
Uranium is the raw material of nuclear power and Nuclear weapons, has vital role to the national economic development and national defense construction.Along with the mankind are to the continuous expansion of energy demand, nuclear energy, as a kind of green energy resource, has very large development space, especially in China.The principal feature of China's uranium ore resource is that ore type is many and complicated, and middle-low grade uranium ore is in the majority, mineralising is uneven, unmanageable uranium ore proportion is larger.In recent years, along with developing rapidly of China's Nuclear Electricity, the imbalance between supply and demand of uranium resources is more outstanding; Conventional acid system leaches owing to there is acid consumption large, the problems such as leaching cycle is long, unfriendly to environment, and uranium concentration is low, and recovery process is complicated, but also needs extra oxygen consumed agent, as sodium chlorate, pyrolusite, can not address this problem far away.Therefore, the situation that uranium resources in China is in short supply be alleviated, must thinking be changed, develop a kind of these type of mineral of new technology process extremely urgent.
Summary of the invention
The above-mentioned defect of uranium ore existence is leached for existing acid system, the invention provides a kind of bacterium agitation leaching method of uranium ore, by cultivating the micro-spirillum of iron protoxide, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans, then carry out the leaching of uranium mineral under agitation, improve the leaching effect of uranium.
The bacterium agitation leaching method of uranium ore of the present invention carries out according to the following steps:
1, be inoculated in 9K substratum by the mixed bacteria liquid containing the micro-spirillum of iron protoxide, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans, make nutrient solution, inoculum size is 1:(10 ~ 20 by the volume ratio of mixed bacteria liquid and 9K substratum);
2, the pH value of nutrient solution is regulated to be 1.0 ~ 2.0 with sulphuric acid soln; In nutrient solution, pass into air and stir and cultivate, air intake is 0.01 ~ 0.1m by often liter of nutrient solution
3/ h, stirring velocity is 600 ~ 1400rpm, and the temperature controlling nutrient solution is 44 ~ 60 DEG C, and the pH value of nutrient solution is 1.0 ~ 2.0, and incubation time is 12 ~ 24h, makes and leaches bacterium liquid;
The ore pulp of the furnishing weight concentration 40 ~ 50% that 3, uranium ore stone flour added water, then with sulphuric acid soln, the pH value of ore pulp is adjusted to 1.0 ~ 2.0, then join and leach in bacterium liquid, make leaching mixed solution, uranium ore stone flour accounts for and leaches 20 ~ 35% of mixed solution gross weight;
4, the pH value leaching mixed solution is regulated to be 1.0 ~ 2.0 with sulphuric acid soln; Pass into air and stir in leaching mixed solution and leach, air intake is 0.01 ~ 0.1m by often liter of nutrient solution
3/ h, stirring velocity is 600 ~ 1400rpm, and the temperature controlling to leach mixed solution is 44 ~ 60 DEG C, and the pH value leaching mixed solution is 1.0 ~ 2.0, and extraction time is 12 ~ 24h.
In above-mentioned uranium ore stone flour, the part of granularity≤74 μm accounts for whole uranium ore stone flour gross weight 60 ~ 90%, and the weight content of uranium is 0.1 ~ 10%.
Leaching yield >=99% of uranium in aforesaid method.
The concentration of above-mentioned sulphuric acid soln is 1 ~ 2N.
In above-mentioned 9K substratum, the concentration of each composition is (NH
4)
2sO
43 g/L, MgSO
47H
2o 0.5 g/L, KCl 0.1 g/L, Ca(NO
3)
20.01 g/L, K
2hPO
40.5 g/L, FeSO
47H
2o 44.2 g/L.
The mixed bacterium that the ore-leaching bacteria that the present invention adopts is made up of three kinds of bacteriums, the micro-spirillum of iron protoxide, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans; These three kinds of bacteriums for the energy, have good oxidation activity with low price iron and reduced sulfur in the temperature range of 44 DEG C ~ 60 DEG C.Method of the present invention can significantly improve the leaching yield of uranium, and reduces acid consumption; By improving leaching ore deposit process kinetics, shortening leaching cycle, improving leaching yield.Method of the present invention has that production cost is low, less investment, equipment are simple, advantages of environment protection.
Embodiment
The micro-spirillum of the iron protoxide adopted in the embodiment of the present invention, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans are bought in China typical culture collection center.
The sulfuric acid preparing sulphuric acid soln employing in the embodiment of the present invention is analytical reagent, and the water of employing is deionized water.
The uranium ore stone flour selected in the embodiment of the present invention is formed after crushed by the uranium ore of mining, and wherein the uranium ore stone flour of weight content more than 2% for pulverizing acquisition again after artificial selection.
In the 9K substratum adopted in the embodiment of the present invention, the concentration of each composition is (NH
4)
2sO
43g/L, MgSO
47H
2o 0.5 g/L, KCl 0.1 g/L, Ca(NO
3)
20.01 g/L, K
2hPO
40.5 g/L, FeSO
47H
2o 44.2 g/L.
In the embodiment of the present invention, the equipment of pH value determination and current potential is PHS-2F type acidometer.
Adopting the container with stirring when preparation is leached bacterium liquid and leaches in the embodiment of the present invention, passing through water bath heat preservation.
Embodiment 1
Be inoculated in 9K substratum by mixed bacteria liquid containing the micro-spirillum of iron protoxide, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans, make nutrient solution, inoculum size is 1:10 by the volume ratio of mixed bacteria liquid and 9K substratum;
The pH value of nutrient solution is regulated to be 1.0 with the sulphuric acid soln that concentration is 2N; In nutrient solution, pass into air and stir and cultivate, air intake is 0.01m by often liter of nutrient solution
3/ h, stirring velocity is 600rpm, and the temperature controlling nutrient solution is 44 ~ 60 DEG C, and the pH value of nutrient solution is 1.0 ~ 2.0, and incubation time is 24h, makes and leaches bacterium liquid;
In the uranium ore stone flour selected, the part of granularity≤74 μm accounts for whole uranium ore stone flour gross weight 90%, and the weight content of uranium is 0.179%;
The ore pulp of furnishing weight concentration 40% that uranium ore stone flour is added water, then with sulphuric acid soln, the pH value of ore pulp is adjusted to 1.0, then join and leach in bacterium liquid, make leaching mixed solution, uranium ore stone flour accounts for and leaches 20% of mixed solution gross weight;
The pH value leaching mixed solution is regulated to be 1.0 ~ 2.0 with the sulphuric acid soln that concentration is 2N; Pass into air and stir in leaching mixed solution and leach, air intake is 0.01m by often liter of nutrient solution
3/ h, stirring velocity is 600rpm, and the temperature controlling to leach mixed solution is 44 ~ 60 DEG C, and the pH value leaching mixed solution is 1.0, and extraction time is 24h; The leaching yield of uranium is 99.85%;
Under condition of the same race, adopt the mode of sulfuric acid leaching not add bacterium immersion and go out uranium ore of the same race, leaching yield is 62.92%.
Embodiment 2
Be inoculated in 9K substratum by mixed bacteria liquid containing the micro-spirillum of iron protoxide, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans, make nutrient solution, inoculum size is 1:15 by the volume ratio of mixed bacteria liquid and 9K substratum;
The pH value of nutrient solution is regulated to be 1.5 with the sulphuric acid soln that concentration is 1N; In nutrient solution, pass into air and stir and cultivate, air intake is 0.02m by often liter of nutrient solution
3/ h, stirring velocity is 1400rpm, and the temperature controlling nutrient solution is 44 ~ 60 DEG C, and the pH value of nutrient solution is 1.0 ~ 2.0, and incubation time is 12h, makes and leaches bacterium liquid;
In the uranium ore stone flour selected, the part of granularity≤74 μm accounts for whole uranium ore stone flour gross weight 81%, and the weight content of uranium is 0.382%
The ore pulp of furnishing weight concentration 50% that uranium ore stone flour is added water, then with sulphuric acid soln, the pH value of ore pulp is adjusted to 1.5, then join and leach in bacterium liquid, make leaching mixed solution, uranium ore stone flour accounts for and leaches 25% of mixed solution gross weight;
The pH value leaching mixed solution is regulated to be 1.0 ~ 2.0 with the sulphuric acid soln that concentration is 1N; Pass into air and stir in leaching mixed solution and leach, air intake is 0.02m by often liter of nutrient solution
3/ h, stirring velocity is 1400rpm, and the temperature controlling to leach mixed solution is 44 ~ 60 DEG C, and the pH value leaching mixed solution is 1.5, and extraction time is 12h; The leaching yield of uranium is 99.37%;
Under condition of the same race, adopt the mode of sulfuric acid leaching not add bacterium immersion and go out uranium ore of the same race, leaching yield is 45.25%.
Embodiment 3
Be inoculated in 9K substratum by mixed bacteria liquid containing the micro-spirillum of iron protoxide, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans, make nutrient solution, inoculum size is 1:20 by the volume ratio of mixed bacteria liquid and 9K substratum;
The pH value of nutrient solution is regulated to be 2.0 with the sulphuric acid soln that concentration is 2N; In nutrient solution, pass into air and stir and cultivate, air intake is 0.03m by often liter of nutrient solution
3/ h, stirring velocity is 800rpm, and the temperature controlling nutrient solution is 44 ~ 60 DEG C, and the pH value of nutrient solution is 1.0 ~ 2.0, and incubation time is 16h, makes and leaches bacterium liquid;
In the uranium ore stone flour selected, the part of granularity≤74 μm accounts for whole uranium ore stone flour gross weight 60%, and the weight content of uranium is 0.527%
The ore pulp of furnishing weight concentration 45% that uranium ore stone flour is added water, then with sulphuric acid soln, the pH value of ore pulp is adjusted to 2.0, then join and leach in bacterium liquid, make leaching mixed solution, uranium ore stone flour accounts for and leaches 30% of mixed solution gross weight;
The pH value leaching mixed solution is regulated to be 1.0 ~ 2.0 with the sulphuric acid soln that concentration is 2N; Pass into air and stir in leaching mixed solution and leach, air intake is 0.03m by often liter of nutrient solution
3/ h, stirring velocity is 800rpm, and the temperature controlling to leach mixed solution is 44 ~ 60 DEG C, and the pH value leaching mixed solution is 2.0, and extraction time is 16h; The leaching yield of uranium is 99.08%;
Under condition of the same race, adopt the mode of sulfuric acid leaching not add bacterium immersion and go out uranium ore of the same race, leaching yield is 23.34%.
Embodiment 4
Be inoculated in 9K substratum by mixed bacteria liquid containing the micro-spirillum of iron protoxide, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans, make nutrient solution, inoculum size is 1:12 by the volume ratio of mixed bacteria liquid and 9K substratum;
The pH value of nutrient solution is regulated to be 1.5 with the sulphuric acid soln that concentration is 1.5N; In nutrient solution, pass into air and stir and cultivate, air intake is 0.015m by often liter of nutrient solution
3/ h, stirring velocity is 1000rpm, and the temperature controlling nutrient solution is 44 ~ 60 DEG C, and the pH value of nutrient solution is 1.0 ~ 2.0, and incubation time is 24h, makes and leaches bacterium liquid;
The uranium ore stone flour selected pulverizes acquisition after being through artificial selection, and the part of granularity≤74 μm accounts for whole uranium ore stone flour gross weight 75%, and the weight content of uranium is 10%
The ore pulp of furnishing weight concentration 50% that uranium ore stone flour is added water, then with sulphuric acid soln, the pH value of ore pulp is adjusted to 1.5, then join and leach in bacterium liquid, make leaching mixed solution, uranium ore stone flour accounts for and leaches 35% of mixed solution gross weight;
The pH value leaching mixed solution is regulated to be 1.5 with the sulphuric acid soln that concentration is 1.5N; Pass into air and stir in leaching mixed solution and leach, air intake is 0.015m by often liter of nutrient solution
3/ h, stirring velocity is 1000rpm, and the temperature controlling to leach mixed solution is 44 ~ 60 DEG C, and the pH value leaching mixed solution is 1.0 ~ 2.0, and extraction time is 24h; The leaching yield of uranium is 99.44%;
Under condition of the same race, adopt the mode of sulfuric acid leaching not add bacterium immersion and go out uranium ore of the same race, leaching yield is 20.12%.
Embodiment 5
Be inoculated in 9K substratum by mixed bacteria liquid containing the micro-spirillum of iron protoxide, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans, make nutrient solution, inoculum size is 1:18 by the volume ratio of mixed bacteria liquid and 9K substratum;
The pH value of nutrient solution is regulated to be 2.0 with the sulphuric acid soln that concentration is 1N; In nutrient solution, pass into air and stir and cultivate, air intake is 0.1m by often liter of nutrient solution
3/ h, stirring velocity is 1400rpm, and the temperature controlling nutrient solution is 44 ~ 60 DEG C, and the pH value of nutrient solution is 1.0 ~ 2.0, and incubation time is 20h, makes and leaches bacterium liquid;
The uranium ore stone flour selected pulverizes acquisition after being through artificial selection, and the part of granularity≤74 μm accounts for whole uranium ore stone flour gross weight 90%, and the weight content of uranium is 7.5%
The ore pulp of furnishing weight concentration 40% that uranium ore stone flour is added water, then with sulphuric acid soln, the pH value of ore pulp is adjusted to 2.0, then join and leach in bacterium liquid, make leaching mixed solution, uranium ore stone flour accounts for and leaches 15% of mixed solution gross weight;
The pH value leaching mixed solution is regulated to be 2.0 with the sulphuric acid soln that concentration is 1N; Pass into air and stir in leaching mixed solution and leach, air intake is 0.1m by often liter of nutrient solution
3/ h, stirring velocity is 1400rpm, and the temperature controlling to leach mixed solution is 44 ~ 60 DEG C, and the pH value leaching mixed solution is 1.0 ~ 2.0, and extraction time is 20h; The leaching yield of uranium is 99.57%;
Under condition of the same race, adopt the mode of sulfuric acid leaching not add bacterium immersion and go out uranium ore of the same race, leaching yield is 29.36%.
Claims (1)
1. a bacterium agitation leaching method for uranium ore, is characterized in that carrying out according to the following steps:
(1) be inoculated in 9K substratum by the mixed bacteria liquid containing the micro-spirillum of iron protoxide, Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans, make nutrient solution, inoculum size is 1:(10 ~ 20 by the volume ratio of mixed bacteria liquid and 9K substratum);
(2) pH value of nutrient solution is regulated to be 1.0 ~ 2.0 with sulphuric acid soln; In nutrient solution, pass into air and stir and cultivate, air intake is 0.01 ~ 0.1m by often liter of nutrient solution
3/ h, stirring velocity is 600 ~ 1400rpm, and the temperature controlling nutrient solution is 44 ~ 60 DEG C, and the pH value of nutrient solution is 1.0 ~ 2.0, and incubation time is 12 ~ 24h, makes and leaches bacterium liquid;
(3) ore pulp of the furnishing weight concentration 40 ~ 50% that uranium ore stone flour added water, then with sulphuric acid soln, the pH value of ore pulp is adjusted to 1.0 ~ 2.0, then join and leach in bacterium liquid, make leaching mixed solution, uranium ore stone flour accounts for and leaches 20 ~ 35% of mixed solution gross weight; In described uranium ore stone flour, the part of granularity≤74 μm accounts for whole uranium ore stone flour gross weight 60 ~ 90%, and the weight content of uranium is 0.1 ~ 10%;
(4) pH value leaching mixed solution is regulated to be 1.0 ~ 2.0 with sulphuric acid soln; Pass into air and stir in leaching mixed solution and leach, air intake is 0.01 ~ 0.1m by often liter of nutrient solution
3/ h, stirring velocity is 600 ~ 1400rpm, and the temperature controlling to leach mixed solution is 44 ~ 60 DEG C, and the pH value leaching mixed solution is 1.0 ~ 2.0, and extraction time is 12 ~ 24h; Leaching yield>=99% of uranium;
Described preparation adopts the container with stirring when leaching bacterium liquid and leach, pass through water bath heat preservation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310579403.1A CN103572047B (en) | 2013-11-19 | 2013-11-19 | A kind of bacterium agitation leaching method of uranium ore |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310579403.1A CN103572047B (en) | 2013-11-19 | 2013-11-19 | A kind of bacterium agitation leaching method of uranium ore |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103572047A CN103572047A (en) | 2014-02-12 |
| CN103572047B true CN103572047B (en) | 2015-11-04 |
Family
ID=50044753
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310579403.1A Active CN103572047B (en) | 2013-11-19 | 2013-11-19 | A kind of bacterium agitation leaching method of uranium ore |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103572047B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2563511C2 (en) * | 2014-05-15 | 2015-09-20 | Виктор Михайлович Курашов | Microbiological method of transmutation of chemical elements and conversion of isotopes of chemical elements |
| CN104046807B (en) * | 2014-06-26 | 2015-12-02 | 东华理工大学 | A kind of pool animalcule leaching uranium method |
| CN105714115B (en) * | 2016-05-09 | 2018-03-13 | 东华理工大学 | A kind of carbonaceous siliceous-pelitic rock type U-ore stone Bioleaching Uranium method |
| CN107058195B (en) * | 2017-06-15 | 2019-12-10 | 东华理工大学 | Composite flora suitable for titanium-uranium ore leaching system and application thereof |
| RU2664005C2 (en) * | 2017-08-23 | 2018-08-14 | Виктор Михайлович Курашов | Microbiological method of obtaining chemical elements and their isotopes, including super-heavy transuranic elements |
-
2013
- 2013-11-19 CN CN201310579403.1A patent/CN103572047B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| "不同搅拌方式下某铀矿石的细菌浸出效果";陈功新等;《金属矿山》;20100831(第8期);第79-81、131页 * |
| "铀矿石不同酸度下细菌的溶浸试验";周仲魁等;《有色金属（冶炼部分）》;20121231(第11期);第52-55页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103572047A (en) | 2014-02-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103572047B (en) | A kind of bacterium agitation leaching method of uranium ore | |
| CN101619401B (en) | Method for recycling uranium from uranium extraction tailings | |
| CN104745498B (en) | A kind of fluorine-resistant ore leaching bacteria and its high-efficiency leaching process applied to high fluorine uranium ore | |
| CN102002588B (en) | Bioleaching method-uranium leaching from fungus | |
| CN105714115B (en) | A kind of carbonaceous siliceous-pelitic rock type U-ore stone Bioleaching Uranium method | |
| CN103396964B (en) | Compound bacterium community capable of efficiently leaching sulphide ore, and compounding method and application method thereof | |
| CN102181627B (en) | Method for treating primary low-grade high-phosphorus manganese ore by acid blending curing | |
| CN103911509B (en) | Two kinds of exclusive leaching ore deposit bacterium are used for Carlin-type gold ore two sections of biological pre-oxidation gold extraction technology | |
| CN108239703B (en) | A kind of electrochemical method and equipment controlling bioleaching process | |
| CN104328280A (en) | Method and equipment for recycling all metal components in waste flexible circuit board | |
| CN101748080A (en) | Ore leaching bacteria and selectivity organism extraction process of low grade zinc sulfide ore | |
| CN102337228B (en) | Thermoacidophile and low grade chalcopyrite ore microbe stepwise leaching process | |
| CN103572048B (en) | Method of cobalt leaching with activated carbon catalysis bacteria | |
| CN104674002B (en) | A kind of Low Grade Copper Ore bioleaching process | |
| CN103805777A (en) | Method of strengthening microbiological leaching of pyrites | |
| CN110616329B (en) | Method for recovering tin from tin-containing waste | |
| CN107739822A (en) | A kind of method of silver ion catalysis copper cobalt sulfide ore Bioleaching | |
| CN107119188A (en) | It is a kind of that the molten method for releasing manganese in extraction manganese oxide ore of diluted acid is reduced based on nitrococcus | |
| CN106222417B (en) | A kind of method that neodymium iron boron waste material rare earth elements are leached using rare earth raffinate | |
| CN103572049B (en) | Bacterium agitation leaching method of cobalt concentrate | |
| CN102020252A (en) | Biological leaching method of low-grade tellurium mine | |
| CN104745496A (en) | Fluoride resistant bacteria and process for using fluoride resistant bacteria for oxidizing Fe<2 +> to Fe<3 +> in fluoride-containing solution | |
| CN101748085B (en) | Biological indium extraction process in sphalerite concentrate ore | |
| CN109112299A (en) | A kind of device and method leached applied to manganese oxide ore biological reducing | |
| CN110029231A (en) | A kind of bacterial leaching way of the complex copper oxide ore containing a variety of copper minerals |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |