CN103565555A - Superovulation method for gayal - Google Patents
Superovulation method for gayal Download PDFInfo
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- CN103565555A CN103565555A CN201210248320.XA CN201210248320A CN103565555A CN 103565555 A CN103565555 A CN 103565555A CN 201210248320 A CN201210248320 A CN 201210248320A CN 103565555 A CN103565555 A CN 103565555A
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Abstract
The invention relates to a superovulation method for a gayal. A multiparity gayal which is at the age of 5-7, weighs 300-800 kg and is healthy, free of disease, normal in sexual cycle, medium in flesh condition and the like is selected as a donor. In any day during an estrus cycle, whether corpus luteum or follicle exists or not, CUE-MATETM is put in the vagina. The day of putting the CUE-MATETM is the first day, and FSH (a group made in Canada and a group made in Japan, and the FSH dosage is divided into three groups of high, medium and low) is injected from the ninth day for 4 days continuously. PG is injected in the third day, then the CUE-MATETM is taken out, semen deposition is conducted for the first time after 48h, semen deposition is conducted for the second time after about 8-16h, and semen deposition is conducted twice. Semen deposition quantity is that 0.25mL/head*time*2 times = 0.50mL/head. The day of the first semen deposition is the day zero, and an embryo is recovered after 6-7 days by means of a non-surgical method. Superovulation and embryo recovery results of 20 heads of gayals are that average corpus luteum quantity, embryo (egg) obtaining quantity and transplantable embryo quantity are 2-17, 1-17 and 1-7.
Description
Technical field
The present invention relates to a kind of gayal Superovulation
.superovulation (superovulation) is called for short super row, refers to that the appropriate time in the estrus cycle is its injection promoting sexual gland hormone female animal. and making has than the technology of more follicular development ovulation under naturalness on its ovary.With HORMONE TREATMENT animal, carrying out superovulation is to carry out one of basis of the research such as embryo transfer, transgenic, clone, can obtain ovum or the germ cell of a large amount of neat, high-qualitys by superovulation.Although at present super row's method is widely used. the raw embryo of super scheduling has 15%～20% (or higher ratio) can not be for transplanting.
Background technology
Variability in the super row's process of donor has limited the industrialization process of embryo transfer, the factor that affects the super row's reaction of donor comprises exopathogenic factor and endogenous cause of ill, exopathogenic factor is as season, nutrition and hormone preparation, and endogenous cause of ill has the physiological status of ovary, particularly follicle ripple stage [1] in ovary when Superovluation.Typical follicle ripple is 8～10d approximately, and in an estrus cycle, the length of follicle wave number amount half-life of corpus luteum in ovary determines.Typical cattle in the estrus cycle approximately 20～30 follicles occur and early development, locking subsequently disappears.Most follicle is capable grew to the ovulation stage, and this point promotes most follicular developments and be confirmed [3] of ovulating by injection exogenous hormone.In ovary, follicle is at least determined by two factors the variability of super row's reaction, the firstth, the follicle quantity of hormonal stimulation growth between super waiting, by super while arranging the beginning in ovary the physiological status of follicle ripple determined.If had superiority in ovary there is the quantity of follicular development in the time of will reducing super row in follicle.The secondth, the follicle number of ovulation after super row, why only some follicle is ovulated, and its mechanism it be not immediately clear, but follicle is also immature or shortage LH is receptor related may occur with LH peak time.The stimulation that LH is accepted in the rear follicle postponement of super row can improve follicle effect of inducing ovulation.This test mainly, on affecting some factors of the super row's reaction of cattle, as nutrition, medicine, dosage are studied, and is found the super discharge method of suitable cattle in conjunction with follicular dynamics principle in ovary, for the industrialization of cattle embryo transfer provides Technical Reference foundation.
Summary of the invention
Select 5～7 years old age, body weight 300～380kg, healthy, without disease, sexual cycle is normal, the growth of livestock is medium multiparity gayal cow, be donor.
Arbitrary day in the estrus cycle, no matter corpus luteum or follicle have or not, by CUE-MATE
tMinsert intravaginal.To put CUE-MATE
tMday be the 1st day, from the 9th day start injection FSH (Canada produces and Japan produces 2 groups, and FSH dosage is divided into high, medium and low 3 groups), inject continuously 4d, at the 3rd day injection PG, take out CUE-MATE simultaneously
tM, after 48h, carry out semen deposition for the first time, semen deposition is for the second time carried out at interval approximately 8～16h, and semen deposition is 2 times altogether.Insemination dose 0.25mL/ head * 2 times=0.50mL/ head.Take day of semen deposition is for the first time 0 day, at 6th～7 days, adopts Nonoperative method to reclaim embryo.
20 super rows and embryo are reclaimed and receive result: average corpus luteum number, obtain that embryo (ovum) is counted, transplantation embryo number is respectively 2～17,1～17 piece and 1～7 piece.
The specific embodiment
The concrete selection of gayal: select 5～7 years old age, body weight 300～380kg, healthy, without disease, sexual cycle is normal, the growth of livestock is medium multiparity gayal cow, be donor.
Supervision Measures
:arbitrary day in the estrus cycle, no matter corpus luteum or follicle have or not, by CUE-MATE
tMinsert intravaginal.To put CUE-MATE
tMday be the 1st day, from the 9th day start injection FSH (Canada produces and Japan produces 2 groups, and FSH dosage is divided into high, medium and low 3 groups), inject continuously 4d, at the 3rd day injection PG, take out CUE-MATE simultaneously
tM, after 48h, carry out semen deposition for the first time, semen deposition is for the second time carried out at interval approximately 8～16h, and semen deposition is 2 times altogether.Insemination dose 0.25mL/ head * 2 times=0.50mL/ head.Take day of semen deposition is for the first time 0 day, at 6th～7 days, adopts Nonoperative method to reclaim embryo.
Assessment
:20 super rows and embryo are reclaimed and receive result: average corpus luteum number, obtain that embryo (ovum) is counted, transplantation embryo number is respectively 2～17,1～17 piece and 1～7 piece.
Claims (3)
1. gayal Superovulation, selects 5～7 years old age, body weight 300～380kg, healthy, without disease, sexual cycle is normal, the growth of livestock is medium multiparity gayal cow, be donor 1.
2. arbitrary day in the estrus cycle according to the gayal of claim 1, no matter corpus luteum or follicle have or not, by CUE-MATE
tMinsert intravaginal.To put CUE-MATE
tMday be the 1st day, from the 9th day start injection FSH (Canada produces and Japan produces 2 groups, and FSH dosage is divided into high, medium and low 3 groups), inject continuously 4d, at the 3rd day injection PG, take out CUE-MATE simultaneously
tM, after 48h, carry out semen deposition for the first time, semen deposition is for the second time carried out at interval approximately 8～16h, and semen deposition is 2 times altogether.Insemination dose 0.25mL/ head * 2 times=0.50mL/ head.Take day of semen deposition is for the first time 0 day, at 6th～7 days, adopts Nonoperative method to reclaim embryo 2.
3. according to the super row of the gayal of claim 1 and embryo: average corpus luteum number, obtain embryo (ovum) number, transplantation embryo number is respectively 2～17,1～17 piece and 1～7 piece 3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210248320.XA CN103565555A (en) | 2012-07-18 | 2012-07-18 | Superovulation method for gayal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210248320.XA CN103565555A (en) | 2012-07-18 | 2012-07-18 | Superovulation method for gayal |
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| Publication Number | Publication Date |
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| CN103565555A true CN103565555A (en) | 2014-02-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201210248320.XA Pending CN103565555A (en) | 2012-07-18 | 2012-07-18 | Superovulation method for gayal |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104800834A (en) * | 2014-01-23 | 2015-07-29 | 中国农业科学院北京畜牧兽医研究所 | Cow superovulation sustained-release agent and preparation method thereof |
-
2012
- 2012-07-18 CN CN201210248320.XA patent/CN103565555A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104800834A (en) * | 2014-01-23 | 2015-07-29 | 中国农业科学院北京畜牧兽医研究所 | Cow superovulation sustained-release agent and preparation method thereof |
| CN104800834B (en) * | 2014-01-23 | 2018-01-19 | 中国农业科学院北京畜牧兽医研究所 | Super row's sustained release agent of a kind of ox and preparation method thereof |
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Application publication date: 20140212 |