CN103555720B - A kind of structure with the yeast strain of production and recycled fiber element restriction endonuclease dual-use function - Google Patents
A kind of structure with the yeast strain of production and recycled fiber element restriction endonuclease dual-use function Download PDFInfo
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- CN103555720B CN103555720B CN201310579729.4A CN201310579729A CN103555720B CN 103555720 B CN103555720 B CN 103555720B CN 201310579729 A CN201310579729 A CN 201310579729A CN 103555720 B CN103555720 B CN 103555720B
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- restriction endonuclease
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- 108091008146 restriction endonucleases Proteins 0.000 title claims abstract description 53
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 24
- 239000000835 fiber Substances 0.000 title claims abstract description 20
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 50
- 239000001913 cellulose Substances 0.000 claims abstract description 30
- 229920002678 cellulose Polymers 0.000 claims abstract description 30
- 239000013612 plasmid Substances 0.000 claims abstract description 28
- 241001225321 Aspergillus fumigatus Species 0.000 claims abstract description 8
- 229940091771 aspergillus fumigatus Drugs 0.000 claims abstract description 8
- 108010042407 Endonucleases Proteins 0.000 claims abstract description 6
- 102000004533 Endonucleases Human genes 0.000 claims abstract description 6
- 230000028327 secretion Effects 0.000 claims abstract description 4
- 108091026890 Coding region Proteins 0.000 claims abstract description 3
- 108700007698 Genetic Terminator Regions Proteins 0.000 claims abstract description 3
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 19
- 229940088598 enzyme Drugs 0.000 description 17
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- 241000588724 Escherichia coli Species 0.000 description 5
- 238000004062 sedimentation Methods 0.000 description 5
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000007979 citrate buffer Substances 0.000 description 3
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
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- MEIRRNXMZYDVDW-MQQKCMAXSA-N (2E,4E)-2,4-hexadien-1-ol Chemical compound C\C=C\C=C\CO MEIRRNXMZYDVDW-MQQKCMAXSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
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- 229920002307 Dextran Polymers 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229910009891 LiAc Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 102000004357 Transferases Human genes 0.000 description 1
- 108090000992 Transferases Proteins 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000567 combustion gas Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
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- -1 dextran glycosides Chemical class 0.000 description 1
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- 229930182470 glycoside Natural products 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
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- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
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- 235000019319 peptone Nutrition 0.000 description 1
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- 238000001556 precipitation Methods 0.000 description 1
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- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
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- 239000011435 rock Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
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Application Number | Priority Date | Filing Date | Title |
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CN201310579729.4A CN103555720B (en) | 2013-11-19 | 2013-11-19 | A kind of structure with the yeast strain of production and recycled fiber element restriction endonuclease dual-use function |
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CN201310579729.4A CN103555720B (en) | 2013-11-19 | 2013-11-19 | A kind of structure with the yeast strain of production and recycled fiber element restriction endonuclease dual-use function |
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CN103555720A CN103555720A (en) | 2014-02-05 |
CN103555720B true CN103555720B (en) | 2015-08-19 |
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CN103789332A (en) * | 2014-03-10 | 2014-05-14 | 湖北工业大学 | Construction of yeast strain having naringinase producing and recycling functions |
CN109182360B (en) * | 2018-10-23 | 2020-06-05 | 怀化学院 | Micromolecular cellulose endonuclease gene and protein and application thereof |
CN110643620B (en) * | 2019-10-22 | 2021-05-28 | 怀化学院 | High-activity poria cocos cellulose endonuclease gene and protein and recombinant vector thereof |
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CN1311082C (en) * | 2001-08-10 | 2007-04-18 | 生物领先公司 | Surface expression vectors having pgs BCA the gene coding poly-gamma-glutamate synthetase, and method for expression of target protein at the surface of microorganism using the vector |
CN101724649B (en) * | 2008-10-29 | 2012-05-02 | 中国科学院上海生命科学研究院 | Method for displaying heterologous proteins on surfaces of cells and product |
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Effective date of registration: 20190802 Address after: 435400 Shifosi Railway Station Industrial Park, Wuxue City, Huanggang City, Hubei Province Patentee after: Wuxue Ganghua Technical Service Center Address before: 430068 Wuhan Province, Wuchang District, South Lake, Lee Ka pier village, No. 1, No. 1, No. Patentee before: HUBEI University OF TECHNOLOGY |
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Effective date of registration: 20191216 Address after: B4-2, 4th floor, Shangdi Information building, No.28, information road, Haidian District, Beijing 100084 Patentee after: Beijing Zhongnonghuawei Biological Technology Co.,Ltd. Address before: 435400 Shifosi Railway Station Industrial Park, Wuxue City, Huanggang City, Hubei Province Patentee before: Wuxue Ganghua Technical Service Center |
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Effective date of registration: 20240328 Address after: 435300 Jinshan Road, Hexi Lishizhen Pharmaceutical Industrial Park, Qichun County, Huanggang City, Hubei Province, No. 008-8 Patentee after: Hubei Shizhen Health Research Co.,Ltd. Country or region after: China Address before: B4-2, 4th floor, Shangdi Information building, 28 Xinhe Road, Haidian District, Beijing 100084 Patentee before: Beijing Zhongnonghuawei Biological Technology Co.,Ltd. Country or region before: China |
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