CN103539498A - Cordyceps militaris culture medium and application thereof - Google Patents
Cordyceps militaris culture medium and application thereof Download PDFInfo
- Publication number
- CN103539498A CN103539498A CN201310462899.4A CN201310462899A CN103539498A CN 103539498 A CN103539498 A CN 103539498A CN 201310462899 A CN201310462899 A CN 201310462899A CN 103539498 A CN103539498 A CN 103539498A
- Authority
- CN
- China
- Prior art keywords
- cordyceps militaris
- link
- culture medium
- medicine
- illumination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a cordyceps militaris culture medium. The cordyceps militaris culture medium comprises the following components: 30g/L of dry silkworm chrysalis meal, 170g/L of rice meal, 1.2g/L of potassium dihydrogen phosphate, 0.8g/L of sodium dihydrogen phosphate and 1g/L of beef extract. The cordyceps militaris culture medium is prepared, sealed, sterilized and cooled; then cordyceps militaris strains are inoculated, strain density is 3-5 pellets/mL, volume ratio of culture medium to strains is 50:1, culture, comprising dark culture and light culture, is carried out; fruiting bodies are harvested. Cordyceps militaris obtained by adopting the cordyceps militaris culture medium is prepared into a medicine, a C57 mouse inoculated with lewiss lung cancer is taken as an experimental subject, administration is carried out once everyday in the standard that administration dosage of cordyceps militaris in the medicine is 5g/kg, and after 10 days, the detected inhibition ratio on the lewiss lung cancer is 19.6%.
Description
Technical field
The present invention relates to Cordyceps militaris (L.) Link. culture technique field, be specifically related to a kind of Chinese caterpillar fungus culture medium, and the application of this Chinese caterpillar fungus culture medium in Cordyceps militaris (L.) Link. is cultivated.
Background technology
Cordyceps militaris (L.) Link. (Cordyceps militaris), has another name called Cordyceps militaris, is under the jurisdiction of Ascomycotina, Clavicipitaceae, the Cordyceps of mycota.It is a kind of medicinal fungi.In Cordyceps militaris (L.) Link., contain sugar, fat and protein, in addition, also contain multiple amino acids, trace element and VITAMIN.The pharmacological actions such as that the multiple physiologically active ingredient in Cordyceps militaris (L.) Link. has is antitumor, antibiotic, anti-oxidant, protection liver, strengthening immunity.And the active substance of itself and Tumor-assaciated comprises cordycepin, polysaccharide, cordycepic acid, sterols, selenium, carotenoid, protein etc.
Yet at present, the Cordyceps militaris (L.) Link. output of artificial culture is limited, its nutrient composition content is low, and quality is uneven, cannot meet people's needs.Especially aspect antitumous effect, adopt extracted its effective constituent and made the health care that medicine carries out the aspect such as antitumor more, and directly with Cordyceps militaris (L.) Link., making medicine, to carry out antineoplastic effect be not very desirable.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of Chinese caterpillar fungus culture medium, uses the medicine antitumous effect that the Cordyccps-militaris-(L.)-link. Sporophore weight that this Chinese caterpillar fungus culture medium is turned out obviously increases, nutrient composition content is high, make obvious.
The technical solution adopted in the present invention is:
, by each component of following content, formed:
Being prepared as follows of this substratum: with pure water by KH
2pO
4, NaH
2pO
4after dissolving, also fully mixing, add again dry dried silkworm chrysalis meal, rice meal, extractum carnis to mix, pack culturing bottle into, sealing, sterilizing, cooling.Wherein sterilising conditions is: 121 ℃, and 1.1Mpa sterilizing 30～45min.
Use above-mentioned Chinese caterpillar fungus culture medium to cultivate the method for Cordyceps militaris (L.) Link., comprise the following steps:
(1) inoculation: inoculate Cordyceps militaris spawn in above-mentioned Chinese caterpillar fungus culture medium, described Cordyceps militaris spawn is Cordyceps militaris (L.) Link., liquid spawn, the density of described Cordyceps militaris spawn is 3～5 bacterium ball/mL, and the volume ratio of described Chinese caterpillar fungus culture medium and Cordyceps militaris spawn is 50:1;
(2) cultivate:
(a) secretly cultivate: under 22 ℃ of temperature, humidity 75～85% conditions, lucifuge is cultivated 3～5 days, to the full described Chinese caterpillar fungus culture medium of Cordyceps militaris (L.) Link. fungus filament length surface;
(b) illumination cultivation adopting for 5th～20 days: fluorescent lamp illumination on 12 hours daytimes, 20～22 ℃ of temperature, humidity 80～85%, intensity of illumination is controlled at 100～150Lx; 12 hours evenings black out, 18～20 ℃ of temperature, humidity 75～85%; Ventilate every day 2 times, sooner or later respectively once, each 20～30 minutes;
(c) illumination cultivation adopting for 21st～35 days: 12 hours daytimes, natural light added fluorescent lamp illumination, 20～22 ℃ of temperature, humidity 75%～85%, total intensity of illumination is controlled at 1000～1200Lx; 12 hours evenings black out, 18～20 ℃ of temperature, humidity 75～85%; Ventilate every day 2 times, sooner or later respectively once, each 20～30 minutes;
In this step (b), (c), cultivate light source used and be scattered light, in culturing process, culturing bottle is carried out to rolling bottle and make illumination even;
(3) results and cultivating again: will the Cordyccps-militaris-(L.)-link. Sporophore of maturation taking-up in culturing bottle after step (3) is cultivated, remainder continues to be cultured to sporophore maturation according to step (3) method (c);
(4) dry: the Cordyccps-militaris-(L.)-link. Sporophore of results is placed in to the Stainless Steel Disc that is lined with gauze, evenly spreads out, 30～37 ℃ of hot air circulation are dried, and its moisture content is controlled at below 10%.
The present invention be take dried silkworm chrysalis meal as main raw material, rice is auxiliary material, add extractum carnis and prepare Chinese caterpillar fungus culture medium as nutritive ingredient, and test the final substratum optimization formula of the present invention of determining by gradient, then adopt special training method: secretly cultivate, then the illumination cultivation mode that different time sections is different, add light source and adopt scattered light, in culturing process, regularly culturing bottle being carried out to rolling bottle makes illumination even, by definite optimum Chinese caterpillar fungus culture medium, fill a prescription, optimize on the whole culture scheme, in strict accordance with the method, cultivating the Cordyccps-militaris-(L.)-link. Sporophore process obtaining detects, find: output increases by 20% than the Cordyccps-militaris-(L.)-link. Sporophore output of using prior art substratum and the cultivation of prior art cultural method to obtain, in every 1g Cordyccps-militaris-(L.)-link. Sporophore, adenosine content is that 15-20mg, cordycepin content are that 7.5-8mg, polysaccharide content are 25-33mg, apparently higher than adopting art methods to cultivate the Cordyceps militaris (L.) Link. obtaining.
The application of the Cordyceps militaris (L.) Link. that aforesaid method of the present invention obtains in the medicine of preparation treatment lung cancer, the Cordyceps militaris (L.) Link. that utilizes the inventive method to cultivate gained is prepared the medicine for the treatment of lung cancer, then carry out antitumor test, found that the inventive method cultivates its anti-lung cancer effect of medicine that the Cordyceps militaris (L.) Link. of gained is prepared into and be significantly better than the medicine that commercially available in the market Cordyceps militaris (L.) Link. is made.It is experimental subjects that the inoculation of take has the C57 mouse of lewiss lung cancer, to after Cordyceps militaris (L.) Link. abrasive dust, make medicine, by the standard that in medicine, Cordyceps militaris (L.) Link. dosage is 5g/kg, carry out administration, administration every day 1 time, after continuous 10 days, detect to such an extent that the inhibiting rate of lewis lung cancer tumour is 19.6%.
Embodiment
Below in conjunction with embodiment, the present invention is further described in detail, but is not limited to this.
Embodiment 1: the preparation of Chinese caterpillar fungus culture medium
Chinese caterpillar fungus culture medium is the aqueous solution, by dry dried silkworm chrysalis meal, rice meal, KH
2pO
4(potassium primary phosphate), NaH
2pO
4(SODIUM PHOSPHATE, MONOBASIC) and extractum carnis form.
Get the composition of following weight: dry dried silkworm chrysalis meal 450g, rice meal 2.55kg, potassium primary phosphate 18g, SODIUM PHOSPHATE, MONOBASIC 12g, extractum carnis 15g; Add pure water 12L first KH
2pO4, NaH
2pO4 adds dry dried silkworm chrysalis meal, rice meal, extractum carnis to mix after first dissolving, also fully mixing again, obtains the culture medium solution that volume is 15L, homogenate, be distributed into culturing bottle, every bottled 50ml, builds lid, 121 ℃, 1.1Mpa sterilizing 30～40min, standby after its natural condensation.
Embodiment 2: the cultivation of Cordyceps militaris (L.) Link.
Use the Chinese caterpillar fungus culture medium of embodiment preparation to cultivate Cordyceps militaris (L.) Link., comprise the following steps:
(1) inoculation: inoculate Cordyceps militaris spawn in the culturing bottle of the Chinese caterpillar fungus culture medium of preparing to embodiment 1 in super clean bench, described Cordyceps militaris spawn is Cordycepsmilitaris (L.) Link., liquid spawn, the density of described Cordyceps militaris spawn is 3～5 bacterium ball/mL, every culturing bottle 1ml;
(2) cultivate:
(a) secretly cultivate: under 22 ℃ of temperature, humidity 75～85% conditions, lucifuge is cultivated 3～5 days, to the full described Chinese caterpillar fungus culture medium of Cordyceps militaris (L.) Link. fungus filament length surface;
(b) illumination cultivation adopting for 5th～20 days: fluorescent lamp illumination on 12 hours daytimes, 20～22 ℃ of temperature, humidity 80～85%, intensity of illumination is controlled at 100～150Lx; 12 hours evenings black out, 18～20 ℃ of temperature, humidity 75～85%; Ventilate every day 2 times, sooner or later respectively once, each 20～30 minutes;
(c) illumination cultivation adopting for 21st～35 days: 12 hours daytimes, natural light added fluorescent lamp illumination, 20～22 ℃ of temperature, humidity 75%～85%, total intensity of illumination is controlled at 1000～1200Lx; 12 hours evenings black out, 18～20 ℃ of temperature, humidity 75～85%; Ventilate every day 2 times, sooner or later respectively once, each 20～30 minutes;
In this step (b), (c), cultivate light source used and be scattered light, in culturing process, regularly culturing bottle is carried out to rolling bottle and make illumination even;
(3) results and cultivating again: will the Cordyccps-militaris-(L.)-link. Sporophore of maturation taking-up in culturing bottle after step (3) is cultivated, remainder continues to be cultured to sporophore maturation according to step (3) method (c);
(4) dry: the Cordyccps-militaris-(L.)-link. Sporophore of results is placed in to the Stainless Steel Disc that is lined with gauze, evenly spreads out, 30～37 ℃ of hot air circulation are dried, and its moisture content is controlled at below 10%.
Precaution in cultivation:
When A, illumination, will use scattered light, and can not use directing light, the growth of Chinese caterpillar fungus has phototropism, to the uniform local bottle of illumination, will carry out rolling bottle, makes its illumination even, and sporophore is growth upwards.
B, in culturing process, to strictly control temperature and humidity, so will strictly control temperature well.The in the situation that of not controlling temperature device in current workshop, sprinkle water to control with ground the temperature of culturing room, watering in general a day 3 times, but also will become according to the temperature situation on the same day, the high Yao Duosa， of temperature culturing room temperature surpasses 25 ℃, the cooling of turning on the aircondition.
C, every day, culturing room wanted ventilation one to twice, and selection of time is relative with dusk temperature low time in the morning, each 20～30 minutes.
The bottle of D, inoculation after stain will be taken out timely, and the substratum of the inside is poured out to renewed vaccination after sterilizing again, can not allow the bottle that pollutes be placed in culturing room and cultivate, and cause whole culturing room to be subject to the pollution of miscellaneous bacteria.
The Cordyceps militaris (L.) Link. that present method is cultivated can gather in the crops for two seasons, and the every bottle of Cordyceps militaris (L.) Link. that at every turn can obtain 1.2g dry weight, has increased by 20% output more at every turn than every bottle of the prior art Cordyceps militaris (L.) Link. that can obtain 1g dry weight.
Embodiment 3: the inhibition test of the medicine that Cordyceps militaris (L.) Link. is made to lewiss lung cancer
Mice with tumor mentioned in embodiment is for inoculating the C57 that has lewiss lung cancer.Mouse C57 mouse is regular grade laboratory animal, male and female dual-purpose, and purchased from Military Medical Science Institute's Experimental Animal Center, Quality of Experimental Animals conformity certification numbering: 2007-004 SCXK-(army).Tumour knurl source lewis lung cancer is by the preservation of going down to posterity of this laboratory.Endoxan sheet, aluminum-plastic packaged, every contains endoxan 50mg, stem and leaf of Radix Ginseng total saponins 50mg, and Tianjin Jinshi Pharmaceutical Co., Ltd. produces, the accurate word H12021006 of traditional Chinese medicines.Sodium chloride injection (physiological saline), bottled, 500mL/ Ping， Qidu Pharmaceutical Co., Ltd., Shandong Prov., product batch number 1D12052502, the accurate word H37020764 of traditional Chinese medicines.
1. the pulverizing of Cordyccps-militaris-(L.)-link. Sporophore
Take respectively the Cordyccps-militaris-(L.)-link. Sporophore of the oven dry of 50g embodiment 2 gained, put into micronized pulverization machine, micronizing 6min, broken whole process as cooling fluid, is guaranteed release and bioactive the stablizing of effective constituent with the water of 4～10 ℃.
2. tumor inoculation
Getting mice with tumor dislocation in good condition in inoculated tumour 10-13 days puts to death, body surface alcohol disinfecting, under aseptic (super clean bench) environment, peel off mouse tumor to the aseptic plate that fills physiological saline, cut to fritter, fritter tumour is put into tissue homogenizer, about 1:3, adding physiological saline homogenate, homogenate is by volume that tumour cell suspension is poured the aseptic centrifuge tube of 50ml into, standby.Viable count 1 * 107/ml concentration, 2ml syringe needle is enclosed within on 1ml syringe, at C57 mouse oxter i.p. inoculation 0.2ml tumour cell suspension, the preparation of tumour cell suspension all under aseptic condition (in Bechtop) complete.From taking out tumour, to tumor inoculation is complete, in 60 minutes, complete.
3. grouping and administration
After tumor cell inoculation, 24 hours all laboratory animal random packet, are divided into 6 groups according to requirement of experiment difference, every treated animal quantity be 10～12 not etc.Experiment is grouped into: 1. physiological saline control group (NS group), 2. positive drug (endoxan) control group, 3.～be 6. respectively medicine, the inventive method that the medicine that commercially available Cordyceps militaris (L.) Link. A makes, the medicine that commercially available Cordyceps militaris (L.) Link. B makes, commercially available Cordyceps militaris (L.) Link. C make to cultivate the medicine that gained Cordyceps militaris (L.) Link. Z makes.
4. reagent configuration:
Get an endoxan sheet, in Yu Yanbo, clay into power, add 40ml physiological saline to mix, packing, configuration in three days is once.Take respectively commercially available Chinese caterpillar fungus A, B, C and present method and cultivate Chinese caterpillar fungus Z powder 1.5g, respectively add 4.5ml physiological saline, the solution that is mixed with 250mg/ml is made medicine, preparation before experiment every day.
5. dosage regimen:
NS group, the physiological saline of the corresponding volume of gavage; 3. the medicine that～medicine that 7. commercially available Cordyceps militaris (L.) Link. A makes, commercially available Cordyceps militaris (L.) Link. B make, the medicine that commercially available Cordyceps militaris (L.) Link. C makes, the inventive method are cultivated the medicine that gained Cordyceps militaris (L.) Link. Z makes, gavage is given the solution of corresponding dosage respectively, and it is 5g/kg that administration volume is 0.4mL/20g(activeconstituents Cordyceps militaris (L.) Link. dosage); Positive drug (endoxan) control group, oral endoxan 25mg/kg(0.4mL/20g); The every average daily administration of each administration group 1 time, continuous 10 days.Within 24 hours after tumor inoculation, start administration.
6. therapeutic evaluation
After last administration 24 hours, put to death animal, take body weight, dissect and take out tumor tissues, take tumor weight, evaluate the vivo antitumor effect of medicine with inhibition rate of tumor growth, calculation formula is: inhibition rate of tumor growth=(the average knurl weight of the average knurl weight/control group of 1-administration group) * 100%.
7. data processing
All data all represents with average and standard deviation.Statistical study, relatively t check between group.Result is as shown in table 1.
Preliminary tumor suppression experimental result demonstration, the inhibiting rate of the medicine group Mouse With Lewis Lung Cancer tumour that the medicine that the medicine that the medicine that commercially available Cordyceps militaris (L.) Link. A makes, commercially available Cordyceps militaris (L.) Link. B make, commercially available Cordyceps militaris (L.) Link. C make, the inventive method cultivation gained Cordyceps militaris (L.) Link. Z make is respectively: 11.8% ,-11.2% ,-8.6%, 20.6%.Comprehensive evaluation, the medicine that the inventive method cultivation gained Cordyceps militaris (L.) Link. is made has and suppresses active mice lung cancer (lewis), the medicine group that wherein the inventive method cultivation gained Cordyceps militaris (L.) Link. is made is compared P value with physiological saline group be 0.009, significant difference, the tumor killing effect effect of drugs that other commercially available Cordyceps militaris (L.) Link. are made is relatively remarkable, and effect certainly.
The above embodiment of the present invention is to explanation of the present invention and can not be for limiting the present invention, and the implication suitable with claims of the present invention and any change in scope, all should think to be included in the scope of claims.
The medicine that each Cordyceps militaris (L.) Link. of table 1 is made is to mouse lewis tumor growth restraining effect
Note: compare * P<0.05, * * P<0.02 with control group.
Claims (3)
1. a Chinese caterpillar fungus culture medium, is characterized in that: each component by following content forms:
2. right to use requires the method that the Chinese caterpillar fungus culture medium described in 1 is cultivated Cordyceps militaris (L.) Link., it is characterized in that comprising the following steps:
(1) inoculation: inoculate Cordyceps militaris spawn in Chinese caterpillar fungus culture medium, described Cordyceps militaris spawn is Cordyceps militaris (L.) Link., liquid spawn, the density of described Cordyceps militaris spawn is 3～5 bacterium ball/mL, and the volume ratio of described Chinese caterpillar fungus culture medium and Cordyceps militaris spawn is 50:1;
(2) cultivate:
(a) secretly cultivate: under 22 ℃ of temperature, humidity 75～85% conditions, lucifuge is cultivated 3～5 days, to the full described Chinese caterpillar fungus culture medium of Cordyceps militaris (L.) Link. fungus filament length surface;
(b) illumination cultivation adopting for 5th～20 days: fluorescent lamp illumination on 12 hours daytimes, 20～22 ℃ of temperature, humidity 80～85%, intensity of illumination is controlled at 100～150Lx; 12 hours evenings black out, 18～20 ℃ of temperature, humidity 75～85%; Ventilate every day 2 times, sooner or later respectively once, each 20～30 minutes;
(c) illumination cultivation adopting for 21st～35 days: 12 hours daytimes, natural light added fluorescent lamp illumination, 20～22 ℃ of temperature, humidity 75%～85%, total intensity of illumination is controlled at 1000～1200Lx; 12 hours evenings black out, 18～20 ℃ of temperature, humidity 75～85%; Ventilate every day 2 times, sooner or later respectively once, each 20～30 minutes;
In this step (b), (c), cultivate light source used and be scattered light, in culturing process, culturing bottle is carried out to rolling bottle and make illumination even;
(3) results and cultivating again: will the Cordyccps-militaris-(L.)-link. Sporophore of maturation taking-up in culturing bottle after step (3) is cultivated, remainder continues to be cultured to sporophore maturation according to step (3) method (c);
(4) dry: the Cordyccps-militaris-(L.)-link. Sporophore of results is placed in to the Stainless Steel Disc that is lined with gauze, evenly spreads out, 30～37 ℃ of hot air circulation are dried, and its moisture content is controlled at below 10%.
3. the application of the Cordyceps militaris (L.) Link. of the method cultivation gained of the Chinese caterpillar fungus culture medium cultivation Cordyceps militaris (L.) Link. described in right to use requirement 1 claimed in claim 2 in the medicine of preparation treatment lung cancer, it is characterized in that: the C57 mouse that inoculates lewiss lung cancer of take is experimental subjects, by Cordyceps militaris (L.) Link. abrasive dust, make medicine, by the standard that in medicine, Cordyceps militaris (L.) Link. dosage is 5g/kg, carry out administration, administration every day 1 time, after continuous 10 days, detect to such an extent that the inhibiting rate of lewis lung cancer tumour is 19.6%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310462899.4A CN103539498B (en) | 2013-09-29 | 2013-09-29 | Cordyceps militaris culture medium and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310462899.4A CN103539498B (en) | 2013-09-29 | 2013-09-29 | Cordyceps militaris culture medium and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103539498A true CN103539498A (en) | 2014-01-29 |
| CN103539498B CN103539498B (en) | 2015-06-17 |
Family
ID=49963411
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310462899.4A Active CN103539498B (en) | 2013-09-29 | 2013-09-29 | Cordyceps militaris culture medium and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103539498B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718036A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris moves cultivating technique |
| CN107475122A (en) * | 2017-07-12 | 2017-12-15 | 华南农业大学 | It is prepared by fan's power bacterium mycoplasma cultural method of a kind of High Yield of Carotenoid and products thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1304995A (en) * | 2001-01-19 | 2001-07-25 | 浙江大学 | Process for culturing cordyceps with domestic silkworm |
| CN1362513A (en) * | 2001-01-04 | 2002-08-07 | 怀化市益康生物高科技有限公司 | Artificial cordyceps culturing medium |
| CN101269110A (en) * | 2007-03-23 | 2008-09-24 | 中天生物科技股份有限公司 | Radix astragali fermentation liquor for inhibiting cancer cell growth and fermentation method |
| CN102515920A (en) * | 2011-12-23 | 2012-06-27 | 正源堂（天津）生物科技有限公司 | Culture of cordyceps militaris and preparation method of oral cordyceps militaris tablet |
| CN102876587A (en) * | 2012-09-27 | 2013-01-16 | 江南大学 | Cordyceps militaris strain for producing cordycepin with high yield |
| KR20130060587A (en) * | 2011-11-30 | 2013-06-10 | 주식회사 진생사이언스 | Food additive comprising extract of cordyceps militaris showing an inhibitory effect of osteoclast differentiation |
-
2013
- 2013-09-29 CN CN201310462899.4A patent/CN103539498B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1362513A (en) * | 2001-01-04 | 2002-08-07 | 怀化市益康生物高科技有限公司 | Artificial cordyceps culturing medium |
| CN1304995A (en) * | 2001-01-19 | 2001-07-25 | 浙江大学 | Process for culturing cordyceps with domestic silkworm |
| CN101269110A (en) * | 2007-03-23 | 2008-09-24 | 中天生物科技股份有限公司 | Radix astragali fermentation liquor for inhibiting cancer cell growth and fermentation method |
| KR20130060587A (en) * | 2011-11-30 | 2013-06-10 | 주식회사 진생사이언스 | Food additive comprising extract of cordyceps militaris showing an inhibitory effect of osteoclast differentiation |
| CN102515920A (en) * | 2011-12-23 | 2012-06-27 | 正源堂（天津）生物科技有限公司 | Culture of cordyceps militaris and preparation method of oral cordyceps militaris tablet |
| CN102876587A (en) * | 2012-09-27 | 2013-01-16 | 江南大学 | Cordyceps militaris strain for producing cordycepin with high yield |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106718036A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris moves cultivating technique |
| CN107475122A (en) * | 2017-07-12 | 2017-12-15 | 华南农业大学 | It is prepared by fan's power bacterium mycoplasma cultural method of a kind of High Yield of Carotenoid and products thereof |
| CN107475122B (en) * | 2017-07-12 | 2021-01-26 | 华南农业大学 | High-carotenoid-yield labyrin mycoplasm culture method and product preparation thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103539498B (en) | 2015-06-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103477871B (en) | Cultivation method and application of cordyceps militaris | |
| CN101760478B (en) | Preparation method of radix puerariae red yeast rice | |
| CN103211212B (en) | A kind of Cordyceps mycelium and preparation method | |
| CN102515920B (en) | Culture of cordyceps militaris and preparation method of oral cordyceps militaris tablet | |
| CN102512458A (en) | Semi-bionic extraction method for active components of Cordyceps militaris | |
| CN103299824B (en) | Method for preparing phellinus igniarius sclertium from fresh, tender and sweet corn | |
| CN103548557A (en) | Method for preparing fleckedflesh polypore hypha block using germinated kernels | |
| CN105664140A (en) | Glycopeptide composition as well as preparation method and application thereof | |
| CN103539498B (en) | Cordyceps militaris culture medium and application thereof | |
| CN106491415A (en) | A kind of pox-eliminating whitening concealer and preparation method thereof | |
| CN1961650B (en) | Xinjiang snow lotus cell tissue cultured substance and method for large-scale subculture | |
| CN101816689B (en) | Processing method of ginseng | |
| CN109498744A (en) | A kind of preparation method that composite bacteria fermentation compound eucommia bark leaf is scattered | |
| CN102670669A (en) | Anti-cancer, anti-virus, anti-fatigue kidney-tonifying selenium-enriched Chinese caterpillar fungus powder and capsule | |
| CN1772007A (en) | Fermented aweto hypha tablet | |
| CN106416747A (en) | Simulative cultivation method of wild ganodorma lucidum | |
| CN106389477B (en) | A kind of preparation method and application of the full cellular plant oil extract of Gordonia terrae | |
| CN112043730A (en) | Traditional Chinese medicine biological preparation for preventing and treating animal epidemic diseases | |
| CN105560374A (en) | Compound preparation for treating dairy cow mammitis and preparation method thereof | |
| TWI379002B (en) | ||
| CN104871827B (en) | Artificially cultivated cordyceps sinensis and cultivation method thereof | |
| TW201506158A (en) | Method for cultivation of cordyceps militaris using supercritical fluid extraction of brown rice | |
| US20210023154A1 (en) | Biological microbial drug for treating skin diseases, and preparation method and application thereof | |
| CN106977300A (en) | A kind of Yellow Fructus Gardeniae special complex fertilizer and preparation method thereof | |
| CN106105780A (en) | A kind of Se-rich pearl Cordyceps production method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |