CN103526554A - Method for antibacterially finishing silk by enzymatic grafted lysozyme - Google Patents
Method for antibacterially finishing silk by enzymatic grafted lysozyme Download PDFInfo
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- CN103526554A CN103526554A CN201210318956.7A CN201210318956A CN103526554A CN 103526554 A CN103526554 A CN 103526554A CN 201210318956 A CN201210318956 A CN 201210318956A CN 103526554 A CN103526554 A CN 103526554A
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Abstract
The invention discloses a method for antibacterially finishing silk by enzymatic grafted lysozyme, belongs to the technical field of textile biology, aims to overcome the defects that silk fibers are easily damaged and the whiteness is reduced caused by high baking temperature in the silk antibacterial treatment in a traditional chemical method, and realizes enzymatic grafted antibacterial functional modification of silk textile. According to the method, tyrosinase has the characteristic of catalytically oxidizing tyrosine residues is utilized, and a lysozyme molecule with a primary amine structure is grafted to the surface of silk fibers, so that the antibacterial effect of silk textile can be improved. The process flow comprises the following steps: pre-treating silk textile; performing tyrosinase catalyzed lysozyme grafting treatment; performing post-treatment of washing and air drying. The antibacterial performance of the silk textile treated by the method can be improved, and the mechanical performance of the textile can be promoted. Compared with a traditional chemical method, the silk textile is subjected to antibacterial treatment by adopting tyrosinase catalytically grafted lysozyme, the processing process is low in energy consumption, high in efficiency and less in pollution, and is advantageous to environment protection.
Description
Technical field
The present invention relates to a kind of method that enzymatic grafting lysozyme carries out real silk antibiotic finish, a kind of catalysed oxidn that utilizes tyrosinase particularly, the lysozyme molecule with antibacterial effect is grafted to cocoon fiber surface, the method of giving real silk fabric antibiotic property, belongs to field of textile biotechnology.
Background technology
Cocoon fiber is mainly comprised of silk gum and fibroin two parts, and in degumming process, water-soluble silk glue is removed, and the large molecule of fibroin is retained, and has given real silk fabric good gloss and feel.Cocoon fiber goods have good hygroscopicity, in taking process, the sweat of human body, sebum and other various secretion easily attach real silk surface, become the good nutrient source of various microorganisms, under suitable external condition (as humidity, temperature etc.), microorganism ramp, breeding, and spread disease by all means, threaten mankind itself's survival and development.On the other hand, cocoon fiber belongs to native protein class, and under suitable temperature, damp condition, itself just easily becomes the nutrient of the microorganisms such as bacterium, mould, thereby silk goods are gone mouldy, and even becomes the carrier of transmission.Along with social fast development and the raising of living standards of the people, people are more and more higher to the requirement of quality of life, and to environmental sanitation and self-healthy pay attention to day by day, the textiles with antibacterial functions is more and more concerned.For improving quality and the wearability of silk fabric, need to carry out antibiotic finish processing to real silk fabric.
At present, the conventional antibacterial finishing agent of silk fabrics comprises inorganic silver ionic species, organic quaternary ammonium salt class and natural polymer subclass etc., the technique of employing comprise pad bake, high temperature adhesives or top finish etc.These finishing agents are when giving the certain antibacterial effect of silk fabrics, also part exists and is combined insecurely with silk fiber, easily from fiber surface, discharges and to migrate to body skin surface, or have that to bake middle fiber strength impaired, fabric feel variation, arranges many drawbacks such as discharging of waste liquid contaminated environment.Lysozyme (Lysozyme) is a kind of novel natural antibacterial agent, is distributed widely in animal, plant and microorganism, has antibiotic property strong, safety non-toxic, the characteristic such as sphere of action is wide.The antibacterial mechanisms of lysozyme is that it can act on microorganism wall, the effective peptide glycan of hydrolytic bacteria cell membrane, make bacteria cell wall become lax, also can be directly virally inactivated in conjunction with making with electronegative virus protein, therefore in textiles antibacterial finishing, there is potential using value.
According to lysozyme, there is the characteristic of good antibacterial, sterilization, if grafted on real silk fabric surface, can set up a kind of novel real silk fabric antibiotic finishing method.In fibroin fiber containing having an appointment 10% tyrosine residue, tyrosinase (Tyrosinase) is as the polyphenol oxidase with catalytic oxidation activity, the phenolic hydroxyl structure of tyrosine residue in energy catalytic oxidation fibroin, formation has reactive quinones structure, cause fibroin and contain the grafting of amino lysozyme molecule, therefore by means of tyrosinase catalysis grafting lysozyme, carrying out real silk antibiotic finish has feasibility.Compare with traditional chemical method, this bio-finishing method is safer, efficient.
Summary of the invention
Object of the present invention aims to provide a kind of eco-friendly real silk fabric bioanalysis antibiotic finishing method.Use the present invention can improve the antibiotic property of real silk fabric, solve the technical problem that in the antibiotic finish of traditional chemical method, energy consumption is high, fibre damage is large and environmental pollution is serious, reach optimization process effect, improve environment, improve the object of true silk products quality.
Technical scheme of the present invention: a kind of method that enzymatic grafting lysozyme carries out real silk antibiotic finish, it is characterized in that the catalysed oxidn by tyrosinase, the lysozyme molecule with antibacterial functions is grafted to cocoon fiber surface, improves the antibiotic property of real silk fabric.
Concrete technology is as follows:
(1) the pre-swelling of real silk: different according to real silk fabric structure tightness after coming unstuck, with disodium ethylene diamine tetraacetate (EDTA)/aqueous solution of urea, selectively carry out silk fabrics swelling pretreatment, fully standby after washing.
Treatment process prescription and condition: EDTA0～1.0g/L, urea 0～1.0g/L, wetting, bleeding agent 0～5g/L, 20～50 ℃ of temperature, process 0～2 hour.
(2) tyrosinase is processed: the real silk fabric with tyrosinase after to swelling pretreatment carries out catalytic oxidation treatment.
Treatment process prescription and condition: tyrosinase 1～200U/g fabric, wetting, bleeding agent 0～5g/L, 20～40 ℃ for the treatment of fluid temperature, pH scope 6.0～8.0, processes 2～24 hours.
(3) lysozyme grafting: take lysozyme as antiseptic, the real silk of processing through tyrosinase is carried out to grafting processing.
Treatment process prescription and condition: lysozyme 0.05～5.0g/L, wetting, bleeding agent 0～5g/L, 20～40 ℃ for the treatment of fluid temperature, pH scope 6.0～8.0, processes 2～24 hours.
(4) post processing: the real silk fabric after enzyme is processed is with the abundant washing of deionized water, low-temperature air-drying be placed in the refrigerator of 0～4 ℃, store standby.
The method that enzymatic grafting lysozyme carries out real silk antibiotic finish, the applicable silk fabrics of processing comprises all kinds of tattings and knitting silk broadcloth, long filament and short fiber silk floss; Enzyme can determine whether to adopt pre-swell process according to real silk fabric breed structure tightness before processing; The enzyme kind that the tyrosinase of application and lysozyme have comprised the separate sources such as animal, plant and microorganism; Enzyme can add Determination of Polyoxyethylene Non-ionic Surfactants, anion surfactant to make wetting, bleeding agent in processing, also can not add any wetting, bleeding agent; Enzyme process in tyrosinase and lysozyme both can add simultaneously, also can add with bathing substep, or adopt two-bath process to process respectively.
Useful consequence of the present invention
The present invention utilizes tyrosinase catalysis oxidation fibroin, realizes lysozyme at silk fabrics surface grafting, reaches the object that improves silk fabrics antibacterial effect, compares with the antibiotic finish of traditional chemical method silk fabrics, and the present invention has the following advantages:
(1) enzymatic efficiency is high, utilizes lysozyme as natural antibacterial finishing agent, usings tyrosinase as the catalyst of enzymatic real silk modification, and in graft reaction, catalytic efficiency is high, and enzyme preparation consumption is few, and enzyme liquid can be realized cycle applications.
(2) reaction condition relaxes, and completes lysozyme in the enzymatic grafting on cocoon fiber surface under the nearly neutrallty condition of low temperature, and while having avoided chemical finishing, high temperature bakes the defect that easily makes silk fiber damage, also less on fabric feeling impact.
(3) utilize tyrosinase catalysis lysozyme at cocoon fiber surface grafting, not only can improve the antibiotic property of fabric, can also promote tyrosine residue and amino covalence bonding on the peptide chain that is connected on fibroin, improved fiber strength.
(4) adopt biological enzyme technology to carry out the ecological processing of real silk fabric functionalization, the low and treatment process safety and environmental protection of energy consumption.
The specific embodiment
Adopt tyrosinase catalysis oxidation fibroin, promote lysozyme at cocoon fiber surface grafting, the specific embodiment that improves silk fabrics anti-microbial property is as follows:
Implement 1: this implements the selected fabric pound Real Silk Habotai of attaching most importance to, and tyrosinase and lysozyme are processed to adopt with bathing substep and carried out.
(1) tyrosinase is processed: the impregnation process 4 hours in tyrosinase solution of the real silk fabric after degumming process, tyrosinase 50U/g fabric wherein, bleeding agent 0.5g/L solution, 30 ℃ of temperature, pH=7.0.
(2) lysozyme grafting: fabric adds lysozyme after tyrosinase is processed 4 hours continues to process 20 hours under lysozyme 1g/L, 30 ℃ of temperature, pH=7 condition.
(3) post processing: enzyme is processed rear silk fabrics with the abundant washing of deionized water, and low-temperature air-drying is standby.
Sample 1: through the blank sample of lysozyme impregnation process;
Sample 2: through tyrosinase, lysozyme treatment samples;
After above-mentioned PROCESS FOR TREATMENT, the inhibiting rate of 1 pair of staphylococcus aureus of sample is 41%, with respect to sample warp-wise strength retention before processing, is 100%, and broadwise strength retention is 98%; The inhibiting rate of 2 pairs of staphylococcus aureuses of sample is 76%, and warp-wise strength retention is 103%, broadwise strength retention 102%.
Implement 2: this implements selected fabric is silk twill, tyrosinase and lysozyme are processed and are adopted two-bath process to carry out respectively.
(1) the pre-swelling of real silk: with EDTA and urea liquid, real silk fabric is carried out to pre-swelling, EDTA 0.2g/L wherein, urea 0.2g/L, bleeding agent 0.5g/L, 40 ℃ of temperature, in 2 hours processing times, process the abundant washing of silk fabrics.
(2) tyrosinase is processed: through the real silk fabric of pre-swelling treatment impregnation process 6 hours in tyrosinase solution, and tyrosinase 10 0U/g fabric wherein, bleeding agent 0.5g/L solution, 30 ℃ of temperature, pH=7.0.
(3) lysozyme grafting: the real silk fabric after tyrosinase is processed takes out, and processes 12 hours, wherein lysozyme 2g/L, 30 ℃ of temperature, pH=6.5 in lysozyme soln.
(4) post processing: enzyme is processed rear silk fabrics with the abundant washing of deionized water, and low-temperature air-drying is standby.
Sample 3: through pre-swelling and the blank sample of lysozyme impregnation process;
Sample 4: through pre-swelling, tyrosinase and lysozyme combined treatment sample;
After above-mentioned PROCESS FOR TREATMENT, the inhibiting rate of 3 pairs of staphylococcus aureuses of sample is 43%, with respect to sample warp-wise strength retention before processing, is 98%, and broadwise strength retention is 95%; The inhibiting rate of 4 pairs of staphylococcus aureuses of sample is 89%, and warp-wise strength retention is 105%, broadwise strength retention 102%.
Claims (5)
1. enzymatic grafting lysozyme carries out a method for real silk antibiotic finish, it is characterized in that utilizing tyrosine residue grafting on tyrosinase catalysis lysozyme molecule and silk fiber, improves the anti-microbial property of real silk fabric.
2. method according to claim 1, is characterized in that concrete steps are as follows:
(1) come unstuck after real silk fabric with disodium ethylene diamine tetraacetate (EDTA)/aqueous solution of urea, carry out the selective swelling pretreatment of silk fabrics, technology preparation and condition: EDTA0～1.0g/L, urea 0～1.0g/L, wetting, bleeding agent 0～5g/L, 20～50 ℃ of temperature, 0～2 hour time; Fully washing after preliminary treatment;
(2) sample that step (1) is processed is with tyrosinase impregnation process, and treatment process is write out a prescription and condition: tyrosinase 1～200U/g fabric, wetting, bleeding agent 0～5g/L solution, 20～40 ℃ of temperature, pH scope 6.0～8.0,2～24 hours time;
(3) sample that step (2) is processed is with lysozyme impregnation process, and treatment process is write out a prescription and condition: lysozyme 0.05～5.0g/L, wetting, bleeding agent 0～5g/L solution, 20～40 ℃ of temperature, pH scope 6.0～8.0,2～24 hours time;
(4) enzyme process after real silk fabric with the abundant washing of deionized water, low-temperature air-drying be placed in the refrigerator of 0～4 ℃, store standby.
3. method according to claim 1, is characterized in that described silk fabrics comprises that all kinds of silk broadcloths, long filament or short fiber are continuous.
4. method according to claim 2, is characterized in that described tyrosinase, lysozyme can derive from the enzyme kind of the separate sources such as animal, plant and microorganism.
5. method according to claim 2, it is characterized in that wherein step (2), (3) process in tyrosinase and lysozyme both can add simultaneously, also can add with bathing substep, or adopt two-bath process to process respectively.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103911857A (en) * | 2014-03-24 | 2014-07-09 | 灵山县桂合丝业有限公司 | Production method of anti-yellow anti-bacterial silk quilt cover |
| CN104631109A (en) * | 2015-02-02 | 2015-05-20 | 浙江理工大学 | Method for catalytic reinforcement of fragile silk fabric by employing aminoacidase |
| CN106758261A (en) * | 2016-11-29 | 2017-05-31 | 江南大学 | A kind of biological enzyme protein fibre fire block articles method for sorting |
| CN107142754A (en) * | 2017-06-01 | 2017-09-08 | 合肥智慧龙图腾知识产权股份有限公司 | A kind of coloring agent of super imitative cotton real silk fabric and preparation method thereof |
| CN111183346A (en) * | 2017-07-12 | 2020-05-19 | 布鲁克道尔顿有限公司 | Humidity stabilization during preparation of biological samples for spectroscopy |
| CN112680974A (en) * | 2020-12-28 | 2021-04-20 | 江南大学 | Protein fiber biological antibacterial finishing method |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103911857A (en) * | 2014-03-24 | 2014-07-09 | 灵山县桂合丝业有限公司 | Production method of anti-yellow anti-bacterial silk quilt cover |
| CN103911857B (en) * | 2014-03-24 | 2016-03-09 | 灵山县桂合丝业有限公司 | A kind of production method of anti-yellowing anti-bacteria real silk quilt cover |
| CN104631109A (en) * | 2015-02-02 | 2015-05-20 | 浙江理工大学 | Method for catalytic reinforcement of fragile silk fabric by employing aminoacidase |
| CN106758261A (en) * | 2016-11-29 | 2017-05-31 | 江南大学 | A kind of biological enzyme protein fibre fire block articles method for sorting |
| CN106758261B (en) * | 2016-11-29 | 2019-05-03 | 江南大学 | A kind of biological enzyme protein fibre fire block articles method for sorting |
| CN107142754A (en) * | 2017-06-01 | 2017-09-08 | 合肥智慧龙图腾知识产权股份有限公司 | A kind of coloring agent of super imitative cotton real silk fabric and preparation method thereof |
| CN111183346A (en) * | 2017-07-12 | 2020-05-19 | 布鲁克道尔顿有限公司 | Humidity stabilization during preparation of biological samples for spectroscopy |
| CN111183346B (en) * | 2017-07-12 | 2023-08-22 | 布鲁克·道尔顿有限及两合公司 | Humidity stabilization during preparation of biological samples for spectrometry |
| CN112680974A (en) * | 2020-12-28 | 2021-04-20 | 江南大学 | Protein fiber biological antibacterial finishing method |
| CN112680974B (en) * | 2020-12-28 | 2022-07-22 | 江南大学 | Protein fiber biological method antibacterial finishing method |
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