CN103526554B - A kind of enzymatic grafting lysozyme carries out the method for real silk antibiotic finish - Google Patents
A kind of enzymatic grafting lysozyme carries out the method for real silk antibiotic finish Download PDFInfo
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- CN103526554B CN103526554B CN201210318956.7A CN201210318956A CN103526554B CN 103526554 B CN103526554 B CN 103526554B CN 201210318956 A CN201210318956 A CN 201210318956A CN 103526554 B CN103526554 B CN 103526554B
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Abstract
The invention discloses a kind of method that enzymatic grafting lysozyme carries out real silk antibiotic finish, belong to field of textile biotechnology.Be intended to solve baking temperature in conventional chemical methods real silk fabric antibacterial treatment high, the defect that silk fiber subject to damage and whiteness decline, realizes the antibacterial functionalized modification of real silk fabric enzymatic.The present invention utilizes tyrosinase to have the characteristic of catalysed oxidn to tyrosine residue in cocoon fiber, and the lysozyme molecule with primary amine structure is grafted to silk fiber surface, improves the antibiotic effect of real silk fabric.Technological process comprises: real silk fabric preliminary treatment, tyrosinase catalysis lysozyme grafting process, washing and air-dry post processing.By the silk fabrics of process of the present invention not only anti-microbial property improve, the mechanics mechanical performance of fabric also has lifting.Compared with traditional chemical routes, adopt tyrosinase catalysis grafting lysozyme to carry out real silk fabric antibacterial treatment, process energy consumption is low, efficiency is high, pollution is few, is conducive to environmental protection.
Description
Technical field
The present invention relates to a kind of method that enzymatic grafting lysozyme carries out real silk antibiotic finish, particularly a kind of catalysed oxidn utilizing tyrosinase, the lysozyme molecule with antibacterial effect is grafted to cocoon fiber surface, give the method for real silk fabric antibiotic property, belong to field of textile biotechnology.
Background technology
Cocoon fiber is primarily of silk gum and fibroin two parts composition, and in degumming process, water-soluble silk glue is removed, and the large molecule of fibroin is retained, and imparts the good gloss of real silk fabric and feel.Cocoon fiber goods have good hygroscopicity, taking in process, the sweat of human body, sebum and other various secretion easily attach real silk surface, become the nutrient source that various microorganism is good, under suitable external condition (as humidity, temperature etc.), microorganism ramp, breeding, and spread disease by all means, threaten the survival and development of mankind itself.On the other hand, cocoon fiber belongs to native protein class, and under suitable temperature, damp condition, itself just easily becomes the nutrient of the microorganism such as bacterium, mould, thus silk goods are gone mouldy, and even becomes the carrier of transmission.Along with the fast development of society and the raising of living standards of the people, the requirement of people to quality of life is more and more higher, and to environmental sanitation and the healthy pay attention to day by day of oneself, the textiles with antibacterial functions is more and more concerned.For improving quality and the wearability of silk fabric, need to carry out antibiotic finish processing to real silk fabric.
At present, the conventional antibacterial finishing agent of silk fabrics comprises inorganic silver ionic species, organic quaternary ammonium salt and natural polymer subclass etc., the technique of employing comprise pad bake, high temperature adhesives or top finish etc.These finishing agents are while the certain antibacterial effect of imparting silk fabrics, also part also exists with silk fiber in conjunction with insecure, and it is impaired that the easily surface from fiber surface release and transport to body skin, or existence bakes middle fiber strength, fabric feel is deteriorated, and arranges many drawbacks such as discharging of waste liquid contaminated environment.Lysozyme (Lysozyme) is a kind of novel natural antibacterial agent, is distributed widely in animal, plant and microorganism, has antibiotic property strong, safety non-toxic, the characteristics such as sphere of action is wide.The antibacterial mechanisms of lysozyme is that it can act on microorganism wall, can the peptide glycan of effective hydrolytic bacteria cell membrane, make bacteria cell wall become lax, also directly can be combined with electronegative virus protein and make virally inactivated, therefore there is in textiles antibacterial finishing potential using value.
There is according to lysozyme the characteristic of excellent antibacterial, sterilization, if grafted on real silk fabric surface, then can set up a kind of novel real silk fabric antibiotic finishing method.In fibroin fiber containing have an appointment 10% tyrosine residue, tyrosinase (Tyrosinase) is as the polyphenol oxidase with catalytic oxidation activity, the phenolic hydroxyl structure of tyrosine residue in energy catalytic oxidation fibroin, formation has reactive quinones structure, cause fibroin and contain the grafting of amino lysozyme molecule, therefore carrying out real silk antibiotic finish by means of tyrosinase catalysis grafting lysozyme and there is feasibility.Compared with traditional chemical routes, this bio-finishing method is safer, efficient.
Summary of the invention
Object of the present invention aims to provide a kind of eco-friendly real silk fabric bioanalysis antibiotic finishing method.Use the present invention can improve the antibiotic property of real silk fabric, solve the technical problem that in conventional chemical methods antibiotic finish, energy consumption is high, fibre damage is large and environmental pollution is serious, reach optimization process effect, environmental protect, improve the object of true silk products quality.
Technical scheme of the present invention: a kind of enzymatic grafting lysozyme carries out the method for real silk antibiotic finish, it is characterized in that the catalysed oxidn by tyrosinase, the lysozyme molecule with antibacterial functions is grafted to cocoon fiber surface, improves the antibiotic property of real silk fabric.
Concrete technology is as follows:
(1) real silk is swelling in advance: different according to real silk fabric structure tightness after coming unstuck, and carries out silk fabrics swelling pretreatment so that disodium ethylene diamine tetraacetate (EDTA)/aqueous solution of urea is selective, for subsequent use after abundant washing.
Treatment process prescription and condition: EDTA0 ~ 1.0g/L, urea 0 ~ 1.0g/L, wetting, bleeding agent 0 ~ 5g/L, temperature 20 ~ 50 DEG C, processes 0 ~ 2 hour.
(2) tyrosinase process: catalytic oxidation treatment is carried out to the real silk fabric after swelling pretreatment with tyrosinase.
Treatment process prescription and condition: tyrosinase 1 ~ 200U/g fabric, wetting, bleeding agent 0 ~ 5g/L, treatment fluid temperature 20 ~ 40 DEG C, pH scope 6.0 ~ 8.0, processes 2 ~ 24 hours.
(3) lysozyme grafting: take lysozyme as antiseptic, grafting process is carried out to the real silk through tyrosinase process.
Treatment process prescription and condition: lysozyme 0.05 ~ 5.0g/L, wetting, bleeding agent 0 ~ 5g/L, treatment fluid temperature 20 ~ 40 DEG C, pH scope 6.0 ~ 8.0, processes 2 ~ 24 hours.
(4) post processing: the real silk fabric after ferment treatment with the abundant washing of deionized water, low-temperature air-drying be placed on store in the refrigerator of 0 ~ 4 DEG C for subsequent use.
Enzymatic grafting lysozyme carries out a method for real silk antibiotic finish, and the silk fabrics being suitable for process comprises all kinds of tatting and knitting silk broadcloth, long filament and short fiber silk floss; Can determine whether adopt pre-swell process according to real silk fabric breed structure tightness before ferment treatment; The tyrosinase of application and lysozyme include the enzyme kind of the separate sources such as animal, plant and microorganism; Determination of Polyoxyethylene Non-ionic Surfactants can be added in ferment treatment, wetting, bleeding agent made by anion surfactant, also can not add any wetting, bleeding agent; In ferment treatment, tyrosinase and lysozyme both can add simultaneously, also can add with bath substep, or adopted two-bath process to process respectively.
Useful consequence of the present invention
The present invention utilizes tyrosinase catalysis to be oxidized fibroin, realizes lysozyme at silk fabrics surface grafting, and reach the object improving silk fabrics antibacterial effect, compared with the antibiotic finish of conventional chemical methods silk fabrics, the present invention has the following advantages:
(1) enzymatic efficiency is high, and utilize lysozyme as natural antibacterial finishing agent, the catalyst using tyrosinase as the modification of enzymatic real silk, in graft reaction, catalytic efficiency is high, and enzyme preparation consumption is few, and enzyme liquid can realize cycle applications.
(2) reaction condition relaxes, and completes the enzymatic grafting of lysozyme on cocoon fiber surface under low temperature near-neutral sulfite deinking, and when avoiding chemical finishing, high temperature bakes the defect easily making silk fiber damage, affects also less on fabric feeling.
(3) utilize tyrosinase catalysis lysozyme at cocoon fiber surface grafting, not only can improve the antibiotic property of fabric, tyrosine residue and amino covalence bonding on the peptide chain that is connected on fibroin can also be promoted, improve fiber strength.
(4) biological enzyme technology is adopted to carry out real silk fabric functionalization Eco-friendly machining, the low and treatment process safety and environmental protection of energy consumption.
Detailed description of the invention
Adopt tyrosinase catalysis oxidation fibroin, promote that lysozyme is at cocoon fiber surface grafting, the specific embodiment improving silk fabrics anti-microbial property is as follows:
Implement 1: the selected fabric of this enforcements is attached most importance to a pound Real Silk Habotai, and tyrosinase and lysozyme process adopt to be carried out step by step with bath.
(1) tyrosinase process: the impregnation process 4 hours in tyrosinase solution of the real silk fabric after degumming process, wherein tyrosinase 50U/g fabric, bleeding agent 0.5g/L solution, temperature 30 DEG C, pH=7.0.
(2) lysozyme grafting: fabric adds lysozyme through tyrosinase process after 4 hours, continues process 20 hours under lysozyme 1g/L, temperature 30 DEG C, pH=7 condition.
(3) post processing: after ferment treatment, silk fabrics is with the abundant washing of deionized water, and low-temperature air-drying is for subsequent use.
Sample 1: through the blank sample of lysozyme impregnation process;
Sample 2: through tyrosinase, lysozyme treatment samples;
After above-mentioned PROCESS FOR TREATMENT, the inhibiting rate of sample 1 pair of staphylococcus aureus is 41%, and be 100% relative to sample warp-wise strength retention before process, broadwise strength retention is 98%; The inhibiting rate of sample 2 pairs of staphylococcus aureuses is 76%, and warp-wise strength retention is 103%, broadwise strength retention 102%.
Implement 2: the selected fabric of this enforcement is silk twill, and tyrosinase and lysozyme process adopt two-bath process to carry out respectively.
(1) real silk is swelling in advance: carry out swelling in advance to real silk fabric with EDTA and urea liquid, wherein EDTA0.2g/L, urea 0.2g/L, bleeding agent 0.5g/L, temperature 40 DEG C, 2 hours processing times, the abundant washing of process silk fabrics.
(2) tyrosinase process: through the real silk fabric of pre-swelling treatment impregnation process 6 hours in tyrosinase solution, wherein tyrosinase 10 0U/g fabric, bleeding agent 0.5g/L solution, temperature 30 DEG C, pH=7.0.
(3) lysozyme grafting: taken out by the real silk fabric after tyrosinase process, processes 12 hours, wherein lysozyme 2g/L, temperature 30 DEG C, pH=6.5 in lysozyme soln.
(4) post processing: after ferment treatment, silk fabrics is with the abundant washing of deionized water, and low-temperature air-drying is for subsequent use.
Sample 3: through the blank sample of swelling and lysozyme impregnation process in advance;
Sample 4: through swelling, tyrosinase and lysozyme combined treatment sample in advance;
After above-mentioned PROCESS FOR TREATMENT, the inhibiting rate of sample 3 pairs of staphylococcus aureuses is 43%, and be 98% relative to sample warp-wise strength retention before process, broadwise strength retention is 95%; The inhibiting rate of sample 4 pairs of staphylococcus aureuses is 89%, and warp-wise strength retention is 105%, broadwise strength retention 102%.
Claims (4)
1. enzymatic grafting lysozyme carries out a method for real silk antibiotic finish, it is characterized in that utilizing tyrosine residue grafting on tyrosinase catalysis lysozyme molecule and cocoon fiber, improves the anti-microbial property of real silk fabric; Concrete steps are as follows:
(1) after coming unstuck, real silk fabric carries out the selective swelling pretreatment of silk fabrics with disodium ethylene diamine tetraacetate (EDTA)/aqueous solution of urea, technology preparation and condition: EDTA0 ~ 1.0g/L, urea 0 ~ 1.0g/L, wetting, bleeding agent 0 ~ 5g/L, temperature 20 ~ 50 DEG C, processes 0 ~ 2 hour; For subsequent use after abundant washing;
(2) sample that processes of step (1) is with tyrosinase impregnation process; Treatment process prescription and condition: tyrosinase 1 ~ 200U/g fabric, wetting, bleeding agent 0 ~ 5g/L solution, temperature 20 ~ 40 DEG C, pH scope 6.0 ~ 8.0,2 ~ 24 hours time;
(3) sample that processes of step (2) is with lysozyme impregnation process; Treatment process prescription and condition: lysozyme 0.05 ~ 5.0g/L, wetting, bleeding agent 0 ~ 5g/L solution, temperature 20 ~ 40 DEG C, pH scope 6.0 ~ 8.0,2 ~ 24 hours time;
(4) after ferment treatment real silk fabric with the abundant washing of deionized water, low-temperature air-drying be placed on store in the refrigerator of 0 ~ 4 DEG C for subsequent use.
2. method according to claim 1, it is characterized in that described real silk fabric comprises with silk is that all kinds of silk broadcloth of raw material or short fiber are continuous.
3. method according to claim 1, is characterized in that described tyrosinase, lysozyme can derive from animal, plant and microorganism.
4. method according to claim 1, is characterized in that in wherein step (2), (3) process, tyrosinase and lysozyme both can add simultaneously, also can add with bath substep, or adopt two-bath process to process respectively.
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| CN103911857B (en) * | 2014-03-24 | 2016-03-09 | 灵山县桂合丝业有限公司 | A kind of production method of anti-yellowing anti-bacteria real silk quilt cover |
| CN104631109A (en) * | 2015-02-02 | 2015-05-20 | 浙江理工大学 | Method for catalytic reinforcement of fragile silk fabric by employing aminoacidase |
| CN106758261B (en) * | 2016-11-29 | 2019-05-03 | 江南大学 | A kind of biological enzyme protein fibre fire block articles method for sorting |
| CN107142754A (en) * | 2017-06-01 | 2017-09-08 | 合肥智慧龙图腾知识产权股份有限公司 | A kind of coloring agent of super imitative cotton real silk fabric and preparation method thereof |
| DE102017119868B4 (en) * | 2017-07-12 | 2021-05-27 | Bruker Daltonik Gmbh | Moisture stabilization when preparing samples for spectrometry |
| CN112680974B (en) * | 2020-12-28 | 2022-07-22 | 江南大学 | Protein fiber biological method antibacterial finishing method |
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