CN103525699B - Special gas accurate controller for cell exposure - Google Patents
Special gas accurate controller for cell exposure Download PDFInfo
- Publication number
- CN103525699B CN103525699B CN201310498112.XA CN201310498112A CN103525699B CN 103525699 B CN103525699 B CN 103525699B CN 201310498112 A CN201310498112 A CN 201310498112A CN 103525699 B CN103525699 B CN 103525699B
- Authority
- CN
- China
- Prior art keywords
- gas
- specific
- cell
- control
- gas cylinder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/14—Pressurized fluid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
Abstract
The invention provides a special gas accurate controller for cell exposure. The special gas accurate controller comprises a stirring mixer, wherein the stirring mixer is provided with three accurate gas flow controlling valves which are connected with a compressed air cylinder, a carbon dioxide gas cylinder and a special equalization gas cylinder, the outer surface of each of the compressed air cylinder, the carbon dioxide gas cylinder and the special equalization gas cylinder is provided with a temperature controlling sleeve, two ends of the special equalization gas cylinder are provided with a vacuum stop valve and an equalization stop valve, the rear end, provided with an electrically controlled three-way valve, of the equalization stop valve is connected with a vacuum pump, the other end of the equalization stop valve is connected to a special total gas source by virtue of a total gas source stop valve, one end of the special equalization gas cylinder is provided with a swing shaft, the swing shaft is connected to an eccentric gear by virtue of a rocker, an output pipeline of the stirring mixer is connected to an exposure gas hole arranged in an exposure gas bin by virtue of an exposure gas flow controlling valve and carries out a quantitative exposure test on the cell on a cell film plate, and a computer controller is used for controlling all electronic valves, motors and total temperature controllers according to a preparatory program to reach the purpose of accurately controlling the special gas during cell exposure. The special gas accurate controller has the beneficial effect of accurately controlling the special gas to carry out scientific research and scientific experiments of cell exposure.
Description
Technical field
The invention provides a kind of cell and expose specific gas precise control device.
Background technology
At present in medical science, environmentalism, military science, in the scientific domains such as toxicology, the specific gas studying some synthetic has been deep into molecules field for the impact of the mankind, in order to special cultured cells or microorganism often can be seeded on special cell lamina membranacea as tested experimental subjects by research in advance that carry out this respect, the specific gas bestowing various dose and concentration on cell lamina membranacea carries out exposure experiment for cell or microorganism, requirement of experiment due to this type of cell or microorganism bestows the flow of specific gas accurately, dosage and concentration and require containing a certain proportion of carbonic acid gas and air, owing to there is no special accurate operating device and method at present to meet above-mentioned requirements, simple manual operation out of true, various gas mixing ratio is uneven, the harsh test conditions of impact etc. also by envrionment temperature brings extreme difficulty to operation, the puzzlement of these problems makes cell expose the scientific research of specific gas and scientific experiment to have to rest on theory speculates or only rough experimental phase by hand, rigorous sh experiment and Theoretical Proof cannot be completed further, serious restriction cell exposes the application and development of specific gas toxicology in this field scientific research, so develop a kind of flow that accurately can control specific gas, the experimental installation that dosage and concentration have the special cell with safety precaution and Growth of Cells conditions warrant to expose specific gas precise control device simultaneously becomes current urgent needs.
Summary of the invention
For solving the problem, the object of this invention is to provide a kind of cell and expose specific gas precise control device, for the scientific domains such as medical science, environmentalism, military science, toxicology provide a kind of flow, dosage and concentration that accurately can control specific gas to have the special cell with safety precaution and Growth of Cells conditions warrant to expose the experimental installation of specific gas precise control device simultaneously.
For achieving the above object, the technical solution used in the present invention be comprise one be placed in negative-pressure cofferdam by stir evenly motor drive stir evenly fan stir evenly mixing tank, stirring evenly on mixing tank has three input apertures to be connected respectively to by pipeline the pressurized air flow control valve that three accurate quantifications control flow, carbon dioxide gas flow control valve and particular airflow control valve, pressurized air flow control valve, the other end of carbon dioxide gas flow control valve and particular airflow control valve is again respectively each via hose connection pneumatic accumulator, dioxide bottle and specific balanced gas cylinder, these three gas cylinder delivery ports there is the manometric pressure loading valve of band, for ensureing the absolutely accurate of volumetric flow of gas under any ambient temperature conditions, these three gas cylinders are all provided with temperature control cover with the outside surface stirring evenly mixing tank, under the control of total temperature controller, all gas volumetric flow rate is all consistent under the consistent prerequisite of temperature, specific balanced gas cylinder one end is provided with vaccum stop valve, the other end is provided with equal balanced stop valve, Electrocontrolled tee valve is equipped with in equal balanced stop valve back, Electrocontrolled tee valve one end connects vacuum pump, the other end is connected to the total source of the gas of specific gas by total source of the gas stopping valve, specific balanced gas cylinder one end is provided with the back shaft that can freely rotate, the other end is provided with swing axle and is connected on eccentric wheel by rocking bar, when turbin generator on eccentric wheel rotates, band movable eccentric wheel rotating rocker drives specific balanced gas cylinder to teeter ceaselessly to be mixed by the specific gas of its inside, ensure the equalization stable that specific gas exports, stir evenly the mixing tank the other end to have a delivery conduit to be connected to after in the aseptic storehouse of malleation to be divided into two, its one end by an airflow balancing control valve release unnecessary mixed gas prevent hypertonia destroy gaseous equilibrium, the other end is connected to by an exposure control damper exposure gas port be arranged in whole exposure module and quantitatively exposes experiment to the cell on cell lamina membranacea, top, exposure gas storehouse in exposure module has special relief outlet outwards to be exported by experiment waste gas, computer control unit is connected to all control dampers by transmission line, Electrocontrolled tee valve, vaccum stop valve, equal balanced stop valve, total source of the gas stopping valve, stir evenly motor, turbin generator and total temperature controller realize according to good program prepared in advance the object that in cell exposure, specific gas accurately controls.
The invention has the beneficial effects as follows that developing a kind of cell exposes specific gas precise control device, the flow of specific gas can be bestowed accurately at molecules field special accuracy control method and equipment, dosage and concentration and require the mixed gas containing a certain proportion of carbonic acid gas and air, after to mix at any time, exposure experiment is not carried out for cell or microorganism by envrionment temperature Recombinant Interferon α-2b, change over simple manual operation out of true, various gas mixing ratio is uneven, impact etc. defect also by environment temperature brings great challenges and puzzlement to operation, the scientific research of cell exposure specific gas and scientific experiment are completed smoothly, facilitate cell and expose the application and development of specific gas toxicology in this field scientific research.
Accompanying drawing explanation
Accompanying drawing is the structural representation that a kind of cell of present device exposes specific gas precise control device
In figure
1, computer control unit 2, pressurized air flow control valve 3, stir evenly motor 4, stir evenly fan
5, airflow balancing control valve 6, exposure control damper 7, temperature control sealing coat 8, relief outlet
9, expose gas storehouse 10, expose gas port 11, cell lamina membranacea 12, liquid-gas interface
13, cell culture fluid 14, incubation groove 15, the aseptic storehouse 16 of malleation, negative pressure isolation cabin
17, temperature control transmission line 18, back shaft 19, eccentric wheel 20, turbin generator
21, rocking bar 22, swing axle 23, the total source of the gas 24 of specific gas, total source of the gas stopping valve
25, vacuum pump 26, automatically controlled transmission line 27, reducing valve 28, pneumatic accumulator
29, temperature control cover 30, CO2 control damper 31, particular airflow control valve 32, specific gas flexible pipe
33, vaccum stop valve 34, all balanced stop valve 35, Electrocontrolled tee valve 36, CO2 gas cylinder
37, specific balanced gas cylinder 38, total temperature controller 39, exposure module 40, stir evenly mixing tank.
Embodiment
By reference to the accompanying drawings and embodiment specific gas precise control device structure exposed to a kind of cell of the present invention be illustrated.
As shown in drawings, the invention provides a kind of cell and expose specific gas precise control device, the negative pressure isolation cabin 16 of this device is the seal isolation storehouse that active window is opened in a front of being made up of insulated heat material, negative pressure isolation cabin 16 wall is provided with temperature control sealing coat 7, the special heating unit being also closely wrapped in temperature control sealing coat 7 outside surface according to shape fabricating is connected to total temperature controller 38 by temperature control transmission line 17, under computer control unit 1 controls, total temperature controller 38 exports different capacity as requested respectively to being wrapped on temperature control cover 29 that temperature control sealing coat 7 outside surface is installed, reach the effect that temperature control is consistent, negative pressure isolation cabin 16 keeps internal space always to remain on negative pressure state by negative pressure pump, to prevent specific gas in experimentation from leaking, operator are damaged, negative pressure isolation cabin 16 is closely connected with the aseptic storehouse 15 of malleation, centre is only separated by one deck temperature control sealing coat 7, various pipeline through all to adopt when negative pressure isolation cabin 16 and the aseptic storehouse 15 of malleation be tightly connected to ensure inside and outside storehouse gas-insulated.Have one be placed in negative-pressure cofferdam 16 by stir evenly motor 3 drive stir evenly fan 4 stir evenly mixing tank 40, stirring evenly mixing tank 40 is the sealed vessel that a corrosion resistant thermally conductive material makes, its shape is made as but is not limited to an egg circle, also can be square or polyhedron-shaped, be equipped with at its top and stir evenly the drive shaft that motor 3 connects a sealing axle sleeve and drive the inner fan 4 that stirs evenly to rotate through sealed vessel, various gas in mixing tank 40 will be stirred evenly stir in time, a delivery port is had at the middle and upper part the other end stirring evenly mixing tank 40, this delivery port is also provided with and blocks up by spring and link block the interface with unidirectional outside ventilatory function forming special construction, be divided into two after in this interface connecting pipeline to the aseptic storehouse 15 of malleation, whole mixing tank 40 periphery that stirs evenly is wrapped in one deck temperature control cover 29.Have three input apertures stirring evenly in the middle part of mixing tank 40, these three input apertures are all provided with spring and link block blocks up the interface with unidirectional inside ventilatory function forming special construction, these three interfaces are connected respectively to pressurized air flow control valve 2 by pipeline, three accurate quantifications of CO2 control damper 30 and particular airflow control valve 31 control the control damper of flow, the other end that these three accurate quantifications control the control damper of flows is again respectively each via hose connection pneumatic accumulator 28, CO2 gas cylinder 36 and specific balanced gas cylinder 37, respectively from pneumatic accumulator 2, three kinds of gases of CO2 gas cylinder 36 and specific balanced gas cylinder 37 are respectively at pressurized air flow control valve 2, the ratio that the program preset according to computer control unit 1 under the accurate quantification control flow of CO2 control damper 30 and these three control dampers of particular airflow control valve 31 requires is input to by these three input apertures and stirs evenly in mixing tank 40, these three gas cylinder delivery ports there are reducing valve 27 and tensimeter, for ensureing the absolutely accurate of volumetric flow of gas under any ambient temperature conditions, these three gas cylinders and the outside surface stirring evenly mixing tank 40 are all provided with temperature control cover 29, under the control of total temperature controller 38, all gas volumetric flow rate is all consistent under the consistent prerequisite of temperature, total temperature controller 38 and temperature control cover 29 are under computer control unit 1 is controlled by automatically controlled transmission line 26 and temperature control transmission line 17, precise hard_drawn tuhes pneumatic accumulator 2 respectively, CO2 gas cylinder 36 and specific balanced gas cylinder 37, stir evenly the professional component of the temperature of mixing tank 40 and temperature control sealing coat 7, temperature control cover 29 is by the soft nest plate adding nichrome wire making in heat conductive rubber, distinguish the pneumatic accumulator 2 of temperature control as requested, CO2 gas cylinder 36 and specific balanced gas cylinder 37, the shape fabricating stirring evenly mixing tank 40 and temperature control sealing coat 7 is also closely wrapped in the special heating unit of its outside surface, total temperature controller 38 is one can export different capacity as requested respectively to being wrapped in pneumatic accumulator 2 under computer control unit 1 controls, CO2 gas cylinder 36 and specific balanced gas cylinder 37, stir evenly mixing tank 40 and reach the consistent effect of temperature control with on the temperature control cover 29 outside temperature control sealing coat 7.Specific balanced gas cylinder 37 is right cylinder high-pressure-resistant vessels that a stainless steel is made, all movable axis is installed at right cylinder outer rim two ends, one end and worktable are fixed as back shaft 18, it is swing axle 22 that the other end connects rocking bar 21, in the eccentric point being connected to eccentric wheel 19 below of rocking bar 21, eccentric wheel 19 center has a turbin generator 20 to be with movable eccentric wheel 19 to rotate, rocking bar 21 moves up and down and causes whole specific balanced gas cylinder 37 to teeter under the drive of eccentric wheel 19, turbin generator 20 requires accurately to control rotating speed according to the program that computer control unit 1 presets by automatically controlled transmission line 26, just can reach and accurately control specific gas ceaselessly Homogeneous phase mixing in specific balanced gas cylinder 37, specific balanced gas cylinder 37 is provided with the Special temp controller cover 29 being closely wrapped in its outside surface, total temperature controller 38 is connected to by temperature control transmission line 17, under computer control unit 1 controls, total temperature controller 38 exports different capacity as requested respectively reach the consistent effect of temperature control to temperature control cover 29, there is semicircular end at specific balanced gas cylinder 37 two ends, the outlet that termination is opened connects a vaccum stop valve 33, the outlet that another termination is opened connects an equal balanced stop valve 34, computer control unit 1 is connected to by automatically controlled transmission line 26, the program that can preset according to computer control unit 1 requires accurately to control to vacuumize and folding during balanced output, Electrocontrolled tee valve 35 is equipped with in equal balanced stop valve 34 back, Electrocontrolled tee valve 35 one end connects vacuum pump, the other end is ended by total source of the gas, 24 are connected to the total source of the gas 23 of specific gas, the other end stirring evenly mixing tank 40 has a delivery conduit to be connected to the aseptic storehouse 15 of malleation, the aseptic storehouse 15 of malleation is seal isolation storehouses that active window is opened in a front of being made up of insulated heat material, the aseptic Cang15Bi of malleation is provided with temperature control sealing coat 7, the special heating unit being also closely wrapped in temperature control sealing coat 7 outside surface according to shape fabricating is connected to total temperature controller 38 by temperature control transmission line 17, under computer control unit 1 controls, total temperature controller 38 exports different capacity as requested respectively to being wrapped on temperature control cover 29 that temperature control sealing coat 7 outside surface is installed, reach the effect that temperature control is consistent, the aseptic storehouse of malleation 15 always remains on barotropic state by maintaining internal space by the positive pressure fan of high efficiency filter, to prevent extraneous bacterium in experimentation from entering, the accuracy affecting experimental result is polluted on experimental cell, the aseptic storehouse 15 of malleation is closely connected with negative pressure isolation cabin 16, centre is only separated by one deck temperature control sealing coat 7, various pipeline through all to adopt when the aseptic storehouse of malleation 15 and negative pressure isolation cabin 16 be tightly connected to ensure inside and outside storehouse gas-insulated.Stir evenly after in the aseptic storehouse 15 of this delivery conduit of mixing tank 40 connection malleation and be divided into two, its one end by an airflow balancing control valve 5 release unnecessary mixed gas prevent hypertonia destroy gaseous equilibrium, the other end is connected to by an exposure control damper 6 the exposure gas port 10 be arranged in whole exposure module 39 and quantitatively exposes experiment to the cell on cell lamina membranacea 11, exposure module 39 is that airtight that a thermally conductive material makes overlap cavity more, ragged edge is the incubation groove 14 having heating unit in, total temperature controller 38 exports specially as requested and meets certain power makes the applicable cells survival of incubation groove 14 temperature maintenance specified temp 37 degrees Celsius to the heating unit in incubation groove 14 under computer control unit 1 controls, there is the cavity holding cell culture fluid incubation groove 14 inside, a cell lamina membranacea 11 infiltrates in the cell culture fluid 13 in the cavity of cell culture fluid 13, but upper surface can not be formed a liquid-gas interface 12 by cell culture fluid 13 submergence, not only can ensure cells survival condition but also be conducive to the exposure test of specific blend gas for cell, directly over cell lamina membranacea 11, be one expose gas storehouse 9, connects the exposure gas port 10 that the pipeline of coming resembles inverted bell mouth shape from the shape that exposure gas storehouse 9 crown center penetrates extend to cell lamina membranacea 11 upper surface top downwards through exposing control damper 6, the program preset according to computer control unit 1 requires the flow accurately controlling the specific gas mixed, dosage and concentration, quantitatively experiment is exposed to the cell on cell lamina membranacea 11 by exposing gas port 10, exposing top, gas storehouse 9 has special relief outlet 8 outwards to be exported by experiment waste gas, computer control unit 1 is connected to pressurized air flow control valve 2 by transmission line, CO2 control damper 30, particular airflow control valve 31, Electrocontrolled tee valve 35, vaccum stop valve 33, equal balanced stop valve 34, total source of the gas stopping valve 24, stir evenly motor 3, turbin generator 20 and total temperature controller 38 realize according to good program prepared in advance the object that in cell exposure, specific gas accurately controls.
Embodiment:
First should determine that the specific gas of requirement of experiment is required before using a kind of cell of the present invention exposure specific gas precise control device to carry out cell exposure test and use physico-chemical property, the specific gas concentration required for experiment is determined according to its physico-chemical property, flow, dosage, the parameters such as temperature are also input in the corresponding program in computer control unit 1 in advance, prepare and the total source of the gas 23 of the good specific gas of installation and debugging, pneumatic accumulator 28, the output pressure of CO2 gas cylinder 36 and specific balanced gas cylinder 37 also connects separately respectively by flexible pipe, open the negative pressure state in the negative-pressure air fan maintenance negative pressure isolation cabin 16 of negative pressure isolation cabin 16, negative pressure isolation cabin 16 is seal isolation storehouses of being made up of insulated heat material, seal isolation bulkhead is provided with temperature control sealing coat 7, the special heating unit being also closely wrapped in temperature control sealing coat 7 outside surface according to shape fabricating is connected to total temperature controller 38 by temperature control transmission line 17, under computer control unit 1 controls, total temperature controller exports different capacity as requested respectively and reaches the consistent effect of temperature control to being wrapped on temperature control cover 29 that temperature control sealing coat 7 outside surface is installed, negative pressure isolation cabin 16 keeps internal space always to remain on negative pressure state by negative pressure pump, to prevent specific gas in experimentation from leaking, operator are damaged, negative pressure isolation cabin 16 is closely connected with the aseptic storehouse 15 of malleation, centre is only separated by one deck temperature control sealing coat 7, various pipeline through all to adopt when the aseptic storehouse of malleation 15 and negative pressure isolation cabin 16 be tightly connected to ensure inside and outside storehouse gas-insulated, the positive pressure fan opening the aseptic storehouse of malleation 15 maintains aseptic barotropic state, because the aseptic storehouse 15 of malleation is seal isolation storehouses that active window is opened in a front of being made up of insulated heat material, seal isolation bulkhead is provided with temperature control sealing coat 7, the special heating unit being also closely wrapped in temperature control sealing coat 7 outside surface according to shape fabricating is connected to total temperature controller 38 by temperature control transmission line 17, under computer control unit 1 controls, total temperature controller 38 exports different capacity as requested respectively and reaches the consistent effect of temperature control to being wrapped on temperature control cover 29 that temperature control sealing coat 7 outside surface is installed, the aseptic storehouse of malleation 15 always remains on barotropic state by maintaining internal space by the positive pressure fan of high efficiency filter, to prevent extraneous bacterium in experimentation from entering, the accuracy affecting experimental result is polluted on experimental cell, with this simultaneously, under computer control unit 1 controls, total temperature controller 38 exports different capacity as requested respectively to being wrapped in pneumatic accumulator 28, CO2 gas cylinder 36 and specific balanced gas cylinder 37, stir evenly mixing tank 40 to control respective temperature make whole system reach the consistent effect of temperature control with on the temperature control cover 29 outside temperature control sealing coat 7, simultaneously total temperature controller 38 under computer control unit 1 controls as requested special export to meet have on the incubation groove 14 of heating unit in airtight of overlapping exposure module 39 ragged edge of cavitys that certain power makes to a thermally conductive material more, heating unit in incubation groove 14 makes incubation groove 14 and whole exposure module 39 temperature keep being applicable to the specified temp 37 degrees Celsius of cells survival, after whole system normally works, take out the cultured subject cell be seeded on cell lamina membranacea 11 in advance, open exposure module 39 upper exposed gas storehouse 9, cell culture fluid 13 is injected the incubation groove 14 in exposure module 39, put into cell lamina membranacea 11 again and build exposure gas storehouse 9, first start-up simulation machine controller 1 controls Electrocontrolled tee valve 35 UNICOM's negative pressure pump and specific balanced gas cylinder 37, open equal balanced stop valve 34 simultaneously and close vaccum stop valve 33, temperature control cover 29 on specific balanced gas cylinder 37 is warmed to suitable true temp to disperse the damp atmosphere in empty bottle by computer control unit 1, open vacuum pump 25 specific balanced gas cylinder 37 is vacuumized and remains dry simultaneously, vacuum pump 25 is closed after arriving certain vacuum degree, Electrocontrolled tee valve 35 is switched to total source of the gas stopping valve 24 one end of the total source of the gas of specific gas 23, open total source of the gas stopping valve 24 make the specific gas in the total source of the gas of specific gas 23 to be full of in specific balanced gas cylinder 37 reach pressure equilibrium after close total source of the gas stopping valve 24, open vaccum stop valve 33 simultaneously, start turbin generator 20 and be with movable eccentric wheel 19, drive rocking bar 21 to move up and down when eccentric wheel 19 rotates also to drive specific balanced gas cylinder 37 to teeter and the specific gas of its inside ceaselessly mixed, ensure the equalization stable that specific gas exports, respectively with hand by pneumatic accumulator 28, suitable output pressure adjusted to by the reducing valve 27 of CO2 gas cylinder 36 and specific balanced gas cylinder 37, at these three pneumatic accumulators 28, reducing valve 27 back of CO2 gas cylinder 36 and specific balanced gas cylinder 37 controls the pressurized air flow control valve 2 of flow respectively each via hose connection accurate quantification, CO2 control damper 30 and these three control dampers of particular airflow control valve 31, the other end that these three accurate quantifications control the control damper of flow is connected respectively to three by pipeline again and is arranged on three input apertures stirring evenly and have in the middle part of mixing tank 40, these three input apertures are and are provided with the interface with unidirectional inside ventilatory function that spring and link block block up composition special construction and can not cause reciprocal leakage and concentration error, the fixing quantity pressurized air flow control valve 2 accurate in scale that computer control unit 1 requires according to the program preset, the control damper that these three accurate quantifications of CO2 control damper 30 and particular airflow control valve 31 control flows according to respective different flow respectively from pneumatic accumulator 28, three kinds of gases of CO2 gas cylinder 36 and specific balanced gas cylinder 37 stir evenly in mixing tank 40 being input to by these three input apertures respectively, stirring evenly mixing tank 40 is the sealed vessel that a corrosion resistant thermally conductive material makes, be equipped with at its top and stir evenly the drive shaft that motor 3 connects a sealing axle sleeve and drive the inner fan 4 that stirs evenly to rotate through sealed vessel, to stir being input to the various gases stirred evenly in mixing tank 40 in time the specific blend gas of equalization stable concentration required for being formed, be divided into two after the other end stirring evenly mixing tank 40 has a delivery conduit to be connected to inside, malleation aseptic storehouse 15, its one end by an airflow balancing control valve 5 release unnecessary mixed gas prevent hypertonia destroy gaseous equilibrium, the other end connects the exposure gas port 10 that the pipeline of coming resembles inverted bell mouth shape from the shape that exposure gas storehouse 9 crown center be arranged in whole exposure module 39 penetrates extend to cell lamina membranacea 11 upper surface top downwards through exposing control damper 6, there is the cavity holding cell culture fluid 13 incubation groove 14 inside in the exposure gas storehouse 9 in whole exposure module 39, a cell lamina membranacea 11 infiltrates in the cavity inner cell nutrient solution 13 of cell culture fluid 13, but upper surface can not be formed a liquid-gas interface 12 by cell culture fluid 13 submergence, not only can ensure cells survival condition but also be conducive to the exposure test of specific blend gas for cell, computer control unit 1 requires according to the program preset the flow of specific gas that accurate Controlled exposure control damper 6 will mix, dosage and concentration quantitatively expose experiment by exposing gas port 10 to the cell on cell lamina membranacea 11, exposing top, gas storehouse 9 has special relief outlet 8 outwards to be exported by experiment waste gas, the process of whole experiment is all required accurately to have controlled according to the program preset by computer control unit 1, experimental arrangement terminates rear computer control unit 1 and controls 31 valves and CO2 control damper 30 and block according to first closing particular airflow the specific gas and CO2 gas that export from specific balanced gas cylinder 37 and CO2 gas cylinder 36, ensure that pressurized air flow control valve 2 is open-minded, the air maintaining pneumatic accumulator 28 continues circulation and supplants each pipeline, after stirring evenly mixing tank 40 and exposing the remaining specific gas in the cavity such as grade of gas storehouse 9, last closes compression air flow control valve 2 again, block the air exported from pneumatic accumulator 28, stop turbin generator 20, stir evenly motor 3, the work of total temperature controller 38 and other all functions, open exposure module 39 upper exposed gas storehouse 9, take out and expose through specific gas the cell lamina membranacea 11 that the inoculation of testing has subject cell, carry out follow-up mensuration and inspection so that obtain the correct conclusion of experimental study.
Claims (6)
1. a cell exposes specific gas precise control device, it is characterized in that: this equipment comprise one be placed in negative-pressure cofferdam (16) by stir evenly motor (3) drive stir evenly fan (4) stir evenly mixing tank (40), negative pressure isolation cabin (16) is closely connected with the aseptic storehouse of malleation (15), centre is only separated by one deck temperature control sealing coat (7), various pipeline through all to adopt when negative pressure isolation cabin (16) and the aseptic storehouse of malleation (15) be tightly connected to ensure inside and outside storehouse gas-insulated, stirring evenly on mixing tank (40) has three input apertures to be connected respectively to by pipeline the pressurized air flow control valve (2) that three accurate quantifications control flow, CO2 control damper (30) and particular airflow control valve (31), pressurized air flow control valve (2), the other end of CO2 control damper (30) and particular airflow control valve (31) is again each via hose connection pneumatic accumulator (28), CO2 gas cylinder (36) and specific balanced gas cylinder (37), these three gas cylinder delivery ports have with manometric reducing valve (27), for ensureing the absolutely accurate of volumetric flow of gas under any ambient temperature conditions, pneumatic accumulator (28), CO2 gas cylinder (36), specific balanced gas cylinder (37) and the outside surface stirring evenly mixing tank (40) are all provided with temperature control cover (29), total temperature controller (38) and temperature control cover (29) are under computer control unit (1) is controlled by automatically controlled transmission line (26) and temperature control transmission line (17), precise hard_drawn tuhes pneumatic accumulator (28) respectively, CO2 gas cylinder (36) and specific balanced gas cylinder (37), stir evenly the professional component of the temperature of mixing tank (40) and temperature control sealing coat (7), temperature control cover (29) is by the soft nest plate adding nichrome wire making in heat conductive rubber, distinguish the pneumatic accumulator (28) of temperature control as requested, CO2 gas cylinder (36) and specific balanced gas cylinder (37), the shape fabricating stirring evenly mixing tank (40) and temperature control sealing coat (7) is also closely wrapped in the special heating unit of its outside surface, total temperature controller (38) exports different capacity as requested respectively and controls to be wrapped in pneumatic accumulator (28) under computer control unit (1) controls, CO2 gas cylinder (36) and specific balanced gas cylinder (37), stir evenly mixing tank (40) and reach the consistent effect of temperature control with the temperature of the temperature control cover (29) of temperature control sealing coat (7) outside, under the control of total temperature controller (38), all gas volumetric flow rate is all consistent under the consistent prerequisite of temperature, specific balanced gas cylinder (37) one end is provided with vaccum stop valve (33), be connected with particular airflow control valve (31) by specific gas flexible pipe (32), the other end is provided with equal balanced stop valve (34), Electrocontrolled tee valve (35) is equipped with in equal balanced stop valve (34) back, Electrocontrolled tee valve (35) one end connects vacuum pump (25), the other end is connected to the total source of the gas of specific gas (23) by total source of the gas stopping valve (24), specific balanced gas cylinder (37) one end is provided with the back shaft (18) that can freely rotate, the other end is provided with swing axle (22) and is connected on eccentric wheel (19) by rocking bar (21), when turbin generator (20) on eccentric wheel (19) rotates, band movable eccentric wheel (19) rotates, rocking bar (21) drives specific balanced gas cylinder (37) to teeter ceaselessly to be mixed by the specific gas of its inside, ensure the equalization stable that specific gas exports, the other end stirring evenly mixing tank (40) has a delivery conduit to be connected to after in the aseptic storehouse of malleation (15) to be divided into two, its one end prevents hypertonia from destroying gaseous equilibrium by airflow balancing control valve (5) unnecessary mixed gas of releasing, the other end exposes control damper (6) by one and is connected to the exposure gas port (10) be arranged in whole exposure module (39), quantitatively experiment is exposed to the cell on cell lamina membranacea (11), exposure gas storehouse (9) top in exposure module (39) has special relief outlet (8) outwards to be exported by experiment waste gas, computer control unit (1) is connected to pressurized air flow control valve (2) by transmission line, CO2 control damper (30), particular airflow control valve (31), Electrocontrolled tee valve (35), vaccum stop valve (33), equal balanced stop valve (34), total source of the gas stopping valve (24), stir evenly motor (3), turbin generator (20) and total temperature controller (38), the object that in cell exposure, specific gas accurately controls is realized according to good program prepared in advance.
2. a kind of cell according to claim 1 exposes specific gas precise control device, it is characterized in that: described in stir evenly mixing tank (40) be the sealed vessel that corrosion resistant thermally conductive material makes, its shape is made as egg circle, square or polyhedron-shaped, be equipped with at its top and stir evenly motor (3), stir evenly motor (3) and connect the drive shaft of a band sealing axle sleeve through sealed vessel, the inner fan (4) that stirs evenly is driven to rotate, various gas in mixing tank (40) will be stirred evenly stir in time, stir evenly mixing tank (40) middle part have three input apertures, these three input apertures are all provided with blocks up by spring and link block the interface with unidirectional inside ventilatory function forming special construction, from pneumatic accumulator (28), three kinds of gases of CO2 gas cylinder (36) and specific balanced gas cylinder (37) are respectively in pressurized air flow control valve (2), the ratio that the program preset according to computer control unit (1) under the accurate quantification control flow of CO2 control damper (30) and particular airflow control valve (31) requires, being input to by these three input apertures stirs evenly in mixing tank (40), a delivery port is had at the middle and upper part the other end stirring evenly mixing tank (40), this delivery port is also provided with and blocks up by spring and link block the interface with unidirectional outside ventilatory function forming special construction, be divided into two after in this interface connecting pipeline to the aseptic storehouse of malleation (15).
3. a kind of cell according to claim 1 exposes specific gas precise control device, it is characterized in that: described specific balanced gas cylinder (37) is the right cylinder high-pressure-resistant vessel that a stainless steel is made, all movable axis is installed at right cylinder outer rim two ends, one end and worktable are fixed as back shaft (18), it is swing axle (22) that the other end connects rocking bar (21), in the eccentric point being connected to eccentric wheel (19) below of rocking bar (21), the center of eccentric wheel (19) has a turbin generator (20) band movable eccentric wheel (19) to rotate, rocking bar (21) moves up and down and causes whole specific balanced gas cylinder (37) to teeter under the drive of eccentric wheel (19), turbin generator (20) requires accurately to control rotating speed according to the program that computer control unit (1) presets by automatically controlled transmission line (26), just can reach and accurately control the interior specific gas of specific balanced gas cylinder (37) ceaselessly Homogeneous phase mixing, specific balanced gas cylinder (37) is provided with the Special temp controller cover (29) being closely wrapped in its outside surface and is connected to total temperature controller (38) by temperature control transmission line (17), there is semicircular end at specific balanced gas cylinder (37) two ends, the outlet that termination is opened connects a vaccum stop valve (33), the outlet that another termination is opened connects an equal balanced stop valve (34) and is connected to computer control unit (1) by automatically controlled transmission line (26), the program preset according to computer control unit (1) requires accurately to control to vacuumize and folding during balanced output.
4. a kind of cell according to claim 1 exposes specific gas precise control device, it is characterized in that: described exposure module (39) is that airtight that a thermally conductive material makes overlap cavity more, ragged edge is the incubation groove (14) having heating unit in, total temperature controller (38) is special as requested under computer control unit (1) controls exports the heating unit met in certain power control incubation groove (14), the temperature of incubation groove (14) is made to keep being applicable to the specified temp 37 degrees Celsius of cells survival, there is the cavity holding cell culture fluid (13) incubation groove (14) inside, a cell lamina membranacea (11) infiltrates in the cell culture fluid (13) in the cavity of cell culture fluid (13), but upper surface can not by cell culture fluid (13) submergence, so just form a liquid-gas interface (12), can either ensure that cells survival condition is conducive to again the exposure test of specific blend gas for cell, directly over cell lamina membranacea (11), be one expose gas storehouse (9), connect the pipeline of coming through exposure control damper (6) and penetrate into from exposure gas storehouse (9) crown center the exposure gas port (10) that shape resembles inverted bell mouth shape, pipeline extends to cell lamina membranacea (11) upper surface top downwards, the program preset according to computer control unit (1) requires the flow accurately controlling the specific gas mixed, dosage and concentration, quantitatively experiment is exposed to the cell on cell lamina membranacea (11) by exposing gas port (10).
5. a kind of cell according to claim 1 exposes specific gas precise control device, it is characterized in that: described negative pressure isolation cabin (16) is the seal isolation storehouse that active window is opened in a front of being made up of insulated heat material, negative pressure isolation cabin (16) wall is provided with temperature control sealing coat (7) and is connected to total temperature controller (38) by temperature control transmission line (17), negative pressure isolation cabin (16) maintains internal space by negative pressure pump and always remains on negative pressure state, damages operator to prevent specific gas in experimentation from leaking.
6. a kind of cell according to claim 1 exposes specific gas precise control device, it is characterized in that: the aseptic storehouse of described malleation (15) is the seal isolation storehouse that active window is opened in a front of being made up of insulated heat material, the aseptic storehouse of malleation (15) wall is provided with temperature control sealing coat (7) and is connected to total temperature controller (38) by temperature control transmission line (17), the aseptic storehouse of malleation (15) always remains on barotropic state by maintaining internal space by the positive pressure fan of high efficiency filter, to prevent extraneous bacterium in experimentation from entering, the accuracy affecting experimental result is polluted on experimental cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310498112.XA CN103525699B (en) | 2013-10-22 | 2013-10-22 | Special gas accurate controller for cell exposure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310498112.XA CN103525699B (en) | 2013-10-22 | 2013-10-22 | Special gas accurate controller for cell exposure |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103525699A CN103525699A (en) | 2014-01-22 |
| CN103525699B true CN103525699B (en) | 2015-03-04 |
Family
ID=49928053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310498112.XA Expired - Fee Related CN103525699B (en) | 2013-10-22 | 2013-10-22 | Special gas accurate controller for cell exposure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103525699B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2016218037B2 (en) * | 2015-02-09 | 2020-12-17 | Univercells Technologies S.A. | System, apparatus and method for the production of cells and/or cell products |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010821A (en) * | 2010-09-02 | 2011-04-13 | 天津开发区合普工贸有限公司 | Cell gaseous exposure experiment control device |
| JP4911487B2 (en) * | 2005-07-12 | 2012-04-04 | 独立行政法人産業技術総合研究所 | Method and apparatus for measuring precise flow rate and calorific value of mixed gas |
| CN102911867A (en) * | 2012-10-09 | 2013-02-06 | 中山大学中山眼科中心 | Cell tissue culture system capable of accurately controlling air pressure and control method thereof |
-
2013
- 2013-10-22 CN CN201310498112.XA patent/CN103525699B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4911487B2 (en) * | 2005-07-12 | 2012-04-04 | 独立行政法人産業技術総合研究所 | Method and apparatus for measuring precise flow rate and calorific value of mixed gas |
| CN102010821A (en) * | 2010-09-02 | 2011-04-13 | 天津开发区合普工贸有限公司 | Cell gaseous exposure experiment control device |
| CN102911867A (en) * | 2012-10-09 | 2013-02-06 | 中山大学中山眼科中心 | Cell tissue culture system capable of accurately controlling air pressure and control method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103525699A (en) | 2014-01-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203683549U (en) | Special gas precise control device for cell exposure | |
| CN208266081U (en) | A kind of biogas residue aerobic compost reactor | |
| WO2019206207A1 (en) | Active ventilation assembly, active-ventilation-type bioreactor and cell culture device | |
| CN103525699B (en) | Special gas accurate controller for cell exposure | |
| CN213951020U (en) | Compost reaction system | |
| CN203839455U (en) | Equipment for filling battery electrolyte in vacuum | |
| CN102443534B (en) | Cell intermittent hypoxic simulation experiment equipment | |
| CN209260093U (en) | Parallel cell culture tank | |
| CN102338727B (en) | Device for measuring diffusion performance of soluble gas in hydrogel | |
| CN210140421U (en) | Automatic sealing device for sample bottle | |
| CN104162372B (en) | Gasometry mixture based on water-gas replacement technique and the fast carrying method of control and device | |
| CN105711260A (en) | Rapid ink filling machine and ink filling method | |
| CN203307880U (en) | Water storing device for experiment | |
| CN109894317A (en) | A kind of cryogenic vacuum immersion system | |
| CN212925008U (en) | High-precision adjustment three-gas culture device for BMSCs low-oxygen treatment | |
| CN111423980B (en) | Fully-closed cell substrate stretching and flow shearing combined loading experiment system | |
| CN212357270U (en) | Automatic IVF culture and time difference analysis device and matched culture dish | |
| CN207694676U (en) | A kind of improvement sealing device of agitating shaft | |
| CN208334315U (en) | Standard gas chamber air distributing device | |
| CN207153514U (en) | A kind of food processing condiment modulating device | |
| CN220405242U (en) | High leakproofness tail gas absorbing device | |
| CN220432804U (en) | Aerobic culture bottle preparation system | |
| CN202983608U (en) | Tank cover device of vacuum stirring tank | |
| CN205426558U (en) | Sterility testing sampling device | |
| CN210287345U (en) | Anaerobic culture device for microbial analysis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 116021 No. 80 Shengli Road, Xigang District, Liaoning, Dalian Applicant after: NO.210 HOSPITAL, PLA Applicant after: Tianjin Development Zone Hepu Industry and Trade Co., Ltd. Address before: 116021 No. 80 Shengli Road, Xigang District, Liaoning, Dalian Applicant before: 210 hospital of PLA Applicant before: Tianjin Development Zone Hepu Industry and Trade Co., Ltd. |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: NO.210 HOSPITAL, PLA TO: PLA NO. 210 HOSPITAL |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150304 Termination date: 20161022 |