CN103521283B - Constant-cold protection system for tissue homogenate extraction - Google Patents
Constant-cold protection system for tissue homogenate extraction Download PDFInfo
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- CN103521283B CN103521283B CN201310453944.XA CN201310453944A CN103521283B CN 103521283 B CN103521283 B CN 103521283B CN 201310453944 A CN201310453944 A CN 201310453944A CN 103521283 B CN103521283 B CN 103521283B
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- cooler
- temperature sensing
- sensing circuit
- cooling
- cooling source
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Abstract
The invention relates to a constant-cold protection system for tissue homogenate extraction. The constant-cold protection system for the tissue homogenate extraction is characterized by comprising a cooler and a cooling source, wherein a centrifugal tube base is arranged in an inner chamber of the cooler; cooling liquid is filled into the cooler; the cooler is communicated with the inner chamber of the cooling source through a first cooling water outlet pipe and a second cooling water inlet pipe respectively; the cooling source comprises a cooling source container, a semiconductor refrigerator, a radiator and a filter; a cold terminal of the semiconductor refrigerator is arranged at the bottom of the cooling source container; a hot terminal of the semiconductor refrigerator is connected with the radiator in a heat-conducting way; a cooling liquid flow speed control pump is arranged on a pipeline of the first cooling water outlet pipe; the cooler is provided with a first temperature sensing circuit; a second temperature sensing circuit is inside the cooling source container; and the first temperature sensing circuit, the second temperature sensing circuit, the cooling liquid flow speed control pump and the semiconductor refrigerator are electrically connected with a controller respectively. The constant-cold protection system for the tissue homogenate extraction has the characteristics of good homoiothermy, capabilities of ensuring that the tissue extracting process is always in the low-temperature state, avoiding changes of properties of tissue protein and nucleic acid and improving the work efficiency, and the like.
Description
Technical field
The present invention relates to a kind of thermostat, particularly Constant-cold protection system for tissue homogenate extraction.
Background technology
Tissue homogenate is the laboratory technique often adopted in modern life science research.In the process of protein extraction, nucleic acid extraction, by tissue homogenate instrument tissue abrasion or cell, the original structure of disorganize and cell, extracts object histone or nucleic acid.These operations usually need to carry out at low temperatures, to prevent the destruction such as histone sex change or nucleic acid cohesion.The Syrup-homogenizing instrument that current experiment room usually adopts is by mechanical spin finishing homogenised tissue.In the process, Syrup-homogenizing instrument grinding head inevitably mechanical friction heat-dissipating, simultaneous grinding head and Junctional tissue also can produce certain heat.Histoclastic albumen and nucleic acid structure is raised for preventing temperature, current experiment Syrup-homogenizing instrument extracts in organizational process, usually need intermittent operation repeatedly, tissue homogenate is taken out at Syrup-homogenizing instrument tip, to make Syrup-homogenizing instrument and tissue cooling, avoid temperature to raise and cause albuminous degeneration, the activity of tissue is extracted in impact.Owing to adopting manual operation, can not ensure that tissue extraction process is in low-temperature condition all the time, histone and nucleic acid proterties be changed, affects operating efficiency simultaneously.
Summary of the invention
The object of this invention is to provide a kind of temperature uniformity good, can ensure that tissue extraction process is in low-temperature condition all the time, avoid histone and nucleic acid proterties to change, the Constant-cold protection system for tissue homogenate extraction of increasing work efficiency.
The object of the present invention is achieved like this, Constant-cold protection system for tissue homogenate extraction, it is characterized in that: comprising: cooler, cooling source, centrifuge tube base is in condenser chamber, cooling fluid is had in cooler, cooler communicates with in cooling source chamber respectively by the first cooling water outlet pipe and the second cooling water inlet pipe, cooling source comprises cooling source container, semiconductor cooler, radiator and filter, the cold pole of semiconductor cooler is at cooling source container bottom, the thermoae of semiconductor cooler is connected with radiator heat conduction, the pipeline of the first cooling water outlet pipe there is cooling liquid speed control pump, cooler has the first temperature sensing circuit, the second temperature sensing circuit is had in cooling source container, first temperature sensing circuit, second temperature sensing circuit, cooling liquid speed control pump and semiconductor cooler are electrically connected with controller respectively, during work, the desired temperature of the first temperature sensing circuit storage that processor stores according to memory, start or close semiconductor cooler drive circuit works, make semiconductor cooler in refrigeration condition or closed condition, simultaneous processor is by control pump drive circuit works, make the coolant flow of cooler and the heat conduction of cooling source phase, the cooling fluid in cooler is processed in the operating temperature of setting always.
Described controller comprises: button, display, processor, pump drive circuit, the first temperature sensing circuit, the second temperature sensing circuit, semiconductor cooler drive circuit and memory, and the first temperature sensing circuit, the second temperature sensing circuit, button, display, pump drive circuit, semiconductor cooler drive circuit and memory are electrically connected with processor interface respectively.
The first described temperature sensing circuit, the second temperature sensing circuit adopt AD590 or thermistor.
Described pump drive circuit, semiconductor cooler drive circuit adopt triode to drive relay circuit, in relay and 12v electric power loop, be connected in series pump or semiconductor cooler.
Advantage of the present invention is: owing to the present invention includes centrifuge tube base, temperature sensor, cooling liquid speed control pump, liquid circulation pipeline, the parts such as cooler, automatic temperature-controlled circulating cooling can be carried out to the centrifuge tube filling tissue substance, simultaneously by the uninterrupted homogenate operation of cooling liquid speed control pump, ensure that tissue extraction process is in low-temperature condition all the time, avoid histone and nucleic acid proterties to change, improve operating efficiency.
Accompanying drawing explanation
Below in conjunction with embodiment accompanying drawing, the invention will be further described:
Fig. 1 is embodiment of the present invention structure principle chart;
Fig. 2 is controller principle block diagram.
In figure, 1, centrifuge tube base; 2, cooling fluid; 3, cooler; 4, the first cooling water outlet pipe; 5, the first temperature sensing circuit; 6, the second temperature sensing circuit; 7, filter; 8, semiconductor cooler; 9, radiator; 10, thermoae; 11, cold pole; 12, cooling liquid speed control pump; 13, supply socket; 14, button; 15, display; 16, controller; 17, cooling source; 18, the second cooling water inlet pipe; 19, cooling source container; 20, processor; 21, pump drive circuit; 22, semiconductor cooler drive circuit; 23, memory.
Detailed description of the invention
As shown in Figure 1, Constant-cold protection system for tissue homogenate extraction
,comprise: cooler 3, cooling source 17, centrifuge tube base 1 is in cooler 3 chamber, cooling fluid 2 is had in cooler 3, cooler 3 communicates with in cooling source 17 chamber respectively by the first cooling water outlet pipe 4 and the second cooling water inlet pipe 18, cooling source 17 comprises cooling source container 19, semiconductor cooler 8, radiator 9 and filter 7, the cold pole 11 of semiconductor cooler 8 is bottom cooling source container 19, thermoae 10 of semiconductor cooler 8 is connected with radiator 9 heat conduction, the pipeline of cooling water outlet pipe 4 has cooling liquid speed control pump 12, cooler 3 has the first temperature sensing circuit 5, the second temperature sensing circuit 6 is had in cooling source container 19, first temperature sensing circuit 5, second temperature sensing circuit 6, cooling liquid speed control pump 12 and semiconductor cooler 8 are electrically connected with controller 16 respectively.First temperature sensing circuit 5, second temperature sensing circuit 6 adopts AD590 or thermistor.Filter 7 is positioned on the outer wall of cooling source container 19, and cools water inlet pipe 18 be communicated with second.Controller 16 is with attaching plug 13.
As shown in Figure 2, described controller 16 comprises: button 14, display 15, processor 20, pump drive circuit 21, first temperature sensing circuit 5, second temperature sensing circuit 6, semiconductor cooler drive circuit 22 and memory 23, first temperature sensing circuit 5, second temperature sensing circuit 6, button 14, display 15, pump drive circuit 21, semiconductor cooler drive circuit 22 and memory 23 are electrically connected with processor 20 interface respectively.
Described pump drive circuit 21, semiconductor cooler drive circuit 22 adopt triode to drive relay circuit, in relay and 12v electric power loop, be connected in series pump or semiconductor cooler.
During work, the desired temperature that the first temperature sensing circuit 5 that processor 20 stores according to memory 23 stores, start or close semiconductor cooler drive circuit 22 and work, make semiconductor cooler 8 in refrigeration condition or closed condition, simultaneous processor 20 is worked by control pump drive circuit 21, the cooling fluid 2 of cooler 3 and the heat conduction of cooling source 17 phase is flowed, the cooling fluid 2 in cooler 3 is processed in the operating temperature of setting always.
The design temperature of cooling fluid 2 has coordinated temperature to set by button 16 and display 15 at first, and design temperature is existed in memory 23.
During work; controller 16 adopts 220v operating voltage; 220v exports 3-5 operating voltage through power circuit and is supplied to processor 20, and power circuit produces operating voltage or the electric current of cooling liquid speed control pump 12 simultaneously, and Constant-cold protection system for tissue homogenate extraction required voltage is met.
The parts that the present embodiment does not describe in detail and structure belong to the well-known components of the industry and common structure or conventional means, do not describe one by one here.
Claims (1)
1. Constant-cold protection system for tissue homogenate extraction, it is characterized in that: comprising: cooler (3), cooling source (17), centrifuge tube base (1) is in cooler (3) chamber, cooling fluid (2) is had in cooler (3), cooler (3) communicates with in cooling source (17) chamber respectively by the first cooling water outlet pipe (4) and the second cooling water inlet pipe (18), cooling source (17) comprises cooling source container (19), semiconductor cooler (8), radiator (9) and filter (7), the cold pole (11) of semiconductor cooler (8) is in cooling source container (19) bottom, thermoae (10) of semiconductor cooler (8) are connected with radiator (9) heat conduction, the pipeline of the first cooling water outlet pipe (4) there is cooling liquid speed control pump (12), cooler (3) has the first temperature sensing circuit (5), the second temperature sensing circuit (6) is had in cooling source container (19), first temperature sensing circuit (5), second temperature sensing circuit (6), cooling liquid speed control pump (12) and semiconductor cooler (8) are electrically connected with controller (16) respectively, during work, the desired temperature that the first temperature sensing circuit (5) that processor (20) stores according to memory (23) stores, start or close semiconductor cooler drive circuit (22) work, make semiconductor cooler (8) in refrigeration condition or closed condition, simultaneous processor (20) is worked by control pump drive circuit (21), the cooling fluid (2) of cooler (3) and cooling source (17) phase heat conduction is flowed, the cooling fluid (2) in cooler (3) is in the operating temperature of setting always, described controller (16) comprising: button (14), display (15), processor (20), pump drive circuit (21), the first temperature sensing circuit (5), the second temperature sensing circuit (6), semiconductor cooler drive circuit (22) and memory (23), and the first temperature sensing circuit (5), the second temperature sensing circuit (6), button (14), display (15), pump drive circuit (21), semiconductor cooler drive circuit (22) and memory (23) are electrically connected with processor (20) interface respectively, described the first temperature sensing circuit (5), the second temperature sensing circuit (6) adopt AD590 or thermistor, described pump drive circuit (21), semiconductor cooler drive circuit (22) adopt triode to drive relay circuit, in relay and 12v electric power loop, be connected in series pump or semiconductor cooler.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310453944.XA CN103521283B (en) | 2013-09-29 | 2013-09-29 | Constant-cold protection system for tissue homogenate extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310453944.XA CN103521283B (en) | 2013-09-29 | 2013-09-29 | Constant-cold protection system for tissue homogenate extraction |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103521283A CN103521283A (en) | 2014-01-22 |
| CN103521283B true CN103521283B (en) | 2015-05-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310453944.XA Expired - Fee Related CN103521283B (en) | 2013-09-29 | 2013-09-29 | Constant-cold protection system for tissue homogenate extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103521283B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2047011U (en) * | 1988-12-21 | 1989-11-01 | 上海制冷设备厂 | High precision cryostat |
-
2013
- 2013-09-29 CN CN201310453944.XA patent/CN103521283B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2047011U (en) * | 1988-12-21 | 1989-11-01 | 上海制冷设备厂 | High precision cryostat |
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| CN103521283A (en) | 2014-01-22 |
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Granted publication date: 20150520 Termination date: 20180929 |