CN103520362B - Heart and brain capsule improves application in medicine preparing learning memory disorder - Google Patents
Heart and brain capsule improves application in medicine preparing learning memory disorder Download PDFInfo
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- CN103520362B CN103520362B CN201310397188.3A CN201310397188A CN103520362B CN 103520362 B CN103520362 B CN 103520362B CN 201310397188 A CN201310397188 A CN 201310397188A CN 103520362 B CN103520362 B CN 103520362B
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Abstract
The invention discloses XINNAOXIN JIAONANG preparing learning memory disorder improves application in medicine.XINNAOXIN JIAONANG causes learning memory disorder model mice ability of learning and memory to scopolamine and has improvement result, and its mechanism of action is the effect of XINNAOXIN JIAONANG by m receptor, and the activity of suppression scopolamine etc. are relevant.
Description
Technical field
The invention belongs to field of medicaments, be specifically related to XINNAOXIN JIAONANG preparing learning memory disorder and improve application in medicine.
Background technology
Study, memory function comprise Spatial memory function and non-space learning and memory function.Dysmnesia model is the effective means that evaluation medicine affects Memory Process, is also the model that discussion Drug therapy senile dementia effect and mechanism of action often adopt.Scopolamine is m receptor blocker, and energy blockage of acetylcholine, to the agonism of m receptor, can be simulated acetylcholine hyposecretion and cause damage in learning and memory.Sodium nitrite can make hemoglobin degeneration, makes brain tissue ischemia anoxia, infringement learning and memory process.Ethanol suppresses corticocerebral function of nervous system movable, and suppress the trained reflex process of animal, make brain internal protein and RNA biosynthesis block, the system such as cholinergic and dopamine changes, and destroys learning and memory function and causes learning and memory to reproduce obstacle.
XINNAOXIN JIAONANG is S&P Pharmaceutical Co., Ltd.'s product, and the accurate word Z20025866 of traditional Chinese medicines, cures mainly supplementing QI and nourishing YIN, blood circulation promoting and blood stasis dispelling.But have not been reported in the effect of learning memory disorder.
Summary of the invention
Goal of the invention: the new opplication that the object of the present invention is to provide XINNAOXIN JIAONANG.
Technical scheme: the invention discloses XINNAOXIN JIAONANG preparing learning memory disorder and improve application in medicine.
Above-mentioned XINNAOXIN JIAONANG is the Radix Rhodiolae of 3 ~ 6 parts by parts by weight, the Fructus Lycii of 5 ~ 15 parts, the Fructus Hippophae composition of 3 ~ 9 parts.
Beneficial effect: show after deliberation, XINNAOXIN JIAONANG causes learning memory disorder model mice ability of learning and memory to scopolamine and has improvement result, and its mechanism of action is the effect of XINNAOXIN JIAONANG by m receptor, and the activity of suppression scopolamine etc. are relevant.
Detailed description of the invention
Embodiment 1:
XINNAOXIN JIAONANG causes memory dysfunction to be improved to chemicals
1 material
1.1 reagent and instrument
XINNAOXIN JIAONANG is purchased from S & P Pharmaceutical Co., Ltd. (lot number 120803); Nimodipine tablet is purchased from Bayer HealthCare Co (lot number BJ09103); Scopolamine hydrobromide alkali is purchased from Sigma-Aldrich(lot number SLBD0119V); Ethanol is purchased from Chemical Reagent Co., Ltd., Sinopharm Group (lot number 20100827); Sodium nitrite is purchased from Nanjing Chemistry Reagent Co., Ltd. (lot number 12060410920).Electronic balance (Beijing Sai Duolisi balance company limited), XT-911 type mice diving tower trained reflex tester (manufacture of Shanghai The 2nd Army Medical College), channel-type water maze
1.2 animal
For examination animal cleaning grade ICR mice, body weight 18-22g, 180, provided by Yangzhou University's comparative medicine center, laboratory animal production licence: SCXK(revives) 2012-0004.Conventional Mus forage feed, freely drinks water.Before experiment, in quiet environment, adaptability is raised 1 week, drinking-water of freely ingesting, and keeps room temperature (22 ± 1 DEG C), natural circadian rhythm illumination.
2 methods
2.1 impacts on scopolamine induced mice memory acquisition disturbance
Mice random packet, if the high, medium and low dosage group of XINNAOXIN JIAONANG, blank group gives isometric(al) distilled water respectively with model group, and positive controls gives nimodipine 30mg/kg, each group difference every day gavage 1 time, successive administration 7 days.
Train by conventional medical research animal model.By XT-911 type mice diving tower trained reflex tester Control of Voltage at 36V, after 1 hour, carry out the test of learning and memory of little mouse function in last administration.Train first 10 minutes model group and administration group lumbar injection scopolamine hydrobromide 0.5mg/kg respectively, the capacity normal saline such as blank group lumbar injection.Often criticize and have 4 mice administrations respectively, operation repetitive, give the 2nd batch of injected in mice again after 10 minutes, by that analogy.To often criticize 4 mices and put into respectively 4 grid of diving tower instrument during training, first conform 3 minutes, be then energized, after mice is shocked by electricity, majority jumps onto diving tower, escapes electric shock.Contacting copper grid for getting an electric shock with mice biped when jumping off simultaneously, being considered as wrong reaction, training 5 minutes, retest after 24 hours.
First be placed on diving tower by mice during test, start timing simultaneously, record mice first time jumps off the time, and this for getting an electric shock incubation period (being error latence), and records in 5 minutes and jump off number of times (being errors number), as observation index.
2.2 impacts on sodium nitrite induced mice remember consolidation disorders
Grouping, administration and training method are with experiment 2.1.Except blank group subcutaneous injection normal saline after training terminates, all the other respectively organize subcutaneous injection sodium nitrite 120mg/kg immediately, test after 24 hours.Method of testing is also with experiment 2.1.
2.3 on 40% ethanol induced mice reproducibility dysmnesia impact
Grouping, administration and training method are with experiment 2.1.Test first 30 minutes, model group and administration group be gavage 40% ethanol 10ml/kg respectively, and blank group gives isometric(al) distilled water.Method of testing is also with experiment 2.1.
3 results
3.1 XINNAOXIN JIAONANG are on the impact of scopolamine induced mice memory acquisition disturbance
3.1.1 give 7d XINNAOXIN JIAONANG (170,340,680mg/kg) continuously, scopolamine induced mice memory acquisition disturbance mice, in channel-type water maze Memory result, XINNAOXIN JIAONANG high dose group can obviously shorten find platform incubation period (
*p<0.01), significantly reduce errors number (
*p<0.01).The results are shown in Table 1.3.1.2 give 7d XINNAOXIN JIAONANG (170,340,680mg/kg) continuously, in mice diving tower Memory result, each dosage group of XINNAOXIN JIAONANG all obviously can extend incubation period, reduce errors number (
*p<0.01).The results are shown in Table 2.
Table 1 XINNAOXIN JIAONANG on the impact of scopolamine induced mice memory acquisition disturbance (channel-type water maze,
n=10)
Compare with blank group: #P<0.05, ##P<0.01; Compare with model group:
*p<0.05,
*p<0.01
Table 2 XINNAOXIN JIAONANG on the impact of scopolamine induced mice memory acquisition disturbance (diving tower,
n=10)
Compare with blank group: #P<0.05, ##P<0.01; Compare with model group:
*p<0.05,
*p<0.01
3.2 XINNAOXIN JIAONANG are on the impact of 40% ethanol induced mice memory acquisition disturbance
3.2.1 7d XINNAOXIN JIAONANG (170,340,680mg/kg) is given continuously, to in 40% ethanol induced mice memory acquisition disturbance mouse experiment, each dosage group of XINNAOXIN JIAONANG can obviously reduce 40% ethanol modeling mice channel-type water maze incubation period and errors number (
*p<0.01).The results are shown in Table 3.
Table 3 XINNAOXIN JIAONANG on the impact of 40% ethanol induced mice memory acquisition disturbance (channel-type water maze,
n=10)
Compare with blank group: #P<0.05, ##P<0.01; Compare with model group:
*p<0.05,
*p<0.01
3.2.2 give 7d XINNAOXIN JIAONANG (170,340,680mg/kg) continuously, in 40% ethanol induced mice memory acquisition disturbance mice Jumping test, XINNAOXIN JIAONANG high dose group obviously can extend incubation period and reduce errors number (
*p<0.05).The results are shown in Table 4.
Table 4 XINNAOXIN JIAONANG on the impact of 40% ethanol induced mice memory acquisition disturbance (diving tower,
n=10)
Compare with blank group: #P<0.05, ##P<0.01; Compare with model group:
*p<0.05,
*p<0.01
3.3 XINNAOXIN JIAONANG are on the impact of sodium nitrite induced mice memory acquisition disturbance
3.3.1 give 7d XINNAOXIN JIAONANG (170,340,680mg/kg) continuously, in sodium nitrite modeling mice channel-type water maze laboratory, XINNAOXIN JIAONANG can obviously reduce model mice find outlet errors number (
*p<0.05).The results are shown in Table 5.
Table 5 XINNAOXIN JIAONANG on the impact of sodium nitrite induced mice memory acquisition disturbance (channel-type water maze,
n=10)
Compare with blank group: #P<0.05, ##P<0.01; Compare with model group:
*p<0.05,
*p<0.01
3.3.2 give 7d XINNAOXIN JIAONANG (170,340,680mg/kg) continuously, in sodium nitrite modeling mice Jumping test, XINNAOXIN JIAONANG does not have obvious effect.The results are shown in Table 6.
Table 6 XINNAOXIN JIAONANG on the impact of sodium nitrite induced mice memory acquisition disturbance (diving tower,
n=10)
Compare with blank group: #P<0.05, ##P<0.01; Compare with model group:
*p<0.05,
*p<0.01
4 conclusions
XINNAOXIN JIAONANG administration group all can shorten the incubation period (P<0.01 or P<0.05) of memory acquisition disturbance model mice caused by scopolamine in various degree; improve spatial memory capacity, reduce and jump off platform and enter cecum errors number (P<0.01); To in 40% ethanol induced mice memory acquisition disturbance mice Jumping test, XINNAOXIN JIAONANG obviously can reduce errors number (P<0.01 or P<0.05), but does not have obvious effect to remember consolidation disorders model caused by sodium nitrite.Summary can obtain XINNAOXIN JIAONANG and be significantly improved memory acquisition disturbance model mice ability of learning and memory caused by scopolamine.
Claims (1)
1. heart and brain capsule improves application in medicine preparing learning memory disorder, it is characterized in that, described heart and brain capsule by parts by weight Radix Rhodiolae 3 ~ 6 parts, Fructus Lycii 5 ~ 15 parts, Fructus Hippophae 3 ~ 9 parts composition.
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Effective date of registration: 20211130 Address after: Is Yuzhong County 730100 Avenue in Lanzhou city in Gansu Province Patentee after: GANSU QIZHENG TIBETAN MEDICINE Co.,Ltd. Address before: 810007 No. 58 Delingha Road, Chengdong District, Xining City, Qinghai Province Patentee before: S&P PHARMACEUTICAL CO.,LTD. |
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