CN103509774B - Manufacturing method of low-bacterium papain - Google Patents
Manufacturing method of low-bacterium papain Download PDFInfo
- Publication number
- CN103509774B CN103509774B CN201310479567.7A CN201310479567A CN103509774B CN 103509774 B CN103509774 B CN 103509774B CN 201310479567 A CN201310479567 A CN 201310479567A CN 103509774 B CN103509774 B CN 103509774B
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- Prior art keywords
- hollow fiber
- papain
- filtration
- group
- ultrafiltration
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- Expired - Fee Related
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 18
- 108090000526 Papain Proteins 0.000 title abstract description 10
- 239000004365 Protease Substances 0.000 title abstract description 10
- 229940055729 papain Drugs 0.000 title abstract description 9
- 235000019834 papain Nutrition 0.000 title abstract description 9
- 239000012510 hollow fiber Substances 0.000 claims abstract description 24
- 235000009467 Carica papaya Nutrition 0.000 claims abstract description 19
- 238000001471 micro-filtration Methods 0.000 claims abstract description 17
- 241000219173 Carica Species 0.000 claims abstract description 16
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 15
- 238000001914 filtration Methods 0.000 claims abstract description 9
- 239000008267 milk Substances 0.000 claims description 14
- 210000004080 milk Anatomy 0.000 claims description 14
- 235000013336 milk Nutrition 0.000 claims description 14
- 239000000835 fiber Substances 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 6
- 230000004899 motility Effects 0.000 claims description 4
- 230000008901 benefit Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 238000004140 cleaning Methods 0.000 abstract description 2
- 239000004816 latex Substances 0.000 abstract 2
- 229920000126 latex Polymers 0.000 abstract 2
- 235000013399 edible fruits Nutrition 0.000 description 4
- 235000006264 Asimina triloba Nutrition 0.000 description 3
- 240000006432 Carica papaya Species 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 2
- 238000004945 emulsification Methods 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000001465 calcium Nutrition 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
- C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
- C12N9/641—Cysteine endopeptidases (3.4.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22002—Papain (3.4.22.2)
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention discloses a manufacturing method of low-bacterium papain. In the manufacturing method, papaya latex is fully utilized; the method comprises the steps of microfiltration and ultrafiltration and is characterized in that in the step of microfiltration, three groups of hollow fiber pipes are sequentially used to perform microfiltration on the papaya latex, wherein the first group of hollow fiber pipes used for filtration is 0.4 micrometer in diameter, the second group of hollow fiber pipes used for filtration is 0.2 micrometer in diameter, and the third group of hollow fiber pipes used for filtration is 0.1 micrometer in diameter; the step of ultrafiltration is that hollow fibers with the molecular weight of 4000 are combined and used for ultrafiltration. The number of microbe of papain prepared through the method is reduced to be lower than or equal to 100; besides, the filtering is fast, the cleaning is easy, and no papain leaks, so that the manufacturing cost of papain can be lowered to a certain extent, and the product quality and the yield of papain are improved.
Description
Technical field
The present invention relates to a kind of production method of proteolytic enzyme, especially the production method of papoid.
Background technology
Pawpaw, also known as papaya or Wanshou fruit, is a kind of tropical fruit.Pawpaw fruit is in yellowish pink orange or reddish orange, and edible rate is high, and containing abundant papain, vitamins C, calcium, carotene and protein and mineral substance etc., nutrition intermediate value is very high.The purposes of papain wherein can be used for food-processing industry, Medicines and Health Product, fodder additives, leather industry and textile industry etc., and purposes widely.Papoid utilizes the milk in immature pawpaw fruit, and a kind of proteolytic ferment adopting biotechnology means to refine, belongs to plant protease, has pure natural, safety non-toxic and the feature such as easy to use.
The method of existing Papain enzyme extraction is generally: papaya milk checked and accepted by raw material
→colloidal mill
→enzyme is separated
→refrigeration → micro-filtration → ultrafiltration → lyophilize → pulverizing → packaging.The problem that this method also exists has: (1) adopts the tubular fibre combination Microfilter of 0.2 μm of hollow fiber conduit to filter papaya milk in microfiltration process always, find in practice, the tubular fibre chimney filter of 0.2 μm easily blocks, cause filtration slow, and the clear liquid leached is through detecting, microbial count >=2000, have exceeded national standard; (2) in ultra-filtration process, carry out ultrafiltration with the tubular fibre combination ultrafilter that the tubular fibre of 6000 molecular weight combines to papaya milk, papoid runs leakage 5%.Have influence on the quality of production and the output of papoid.
Summary of the invention
The object of this invention is to provide a kind of production method of low bacterium height motility rate papoid, improve the quality of production and the output of extracting papoid from papaya milk.
For reaching above-mentioned purpose, technical scheme of the present invention is:
A production method for low bacterium height motility rate papoid, it takes full advantage of papaya milk, comprises micro-filtration and ultrafiltration step, it is characterized in that:
Described microfiltration step, sequentially with three groups of hollow fiber conduits, micro-filtration is carried out to papaya milk: first group with 0.4 μm of hollow fiber conduit filter, second group with 0.2 μm of hollow fiber conduit filter, the 3rd group with 0.1 μm of hollow fiber conduit filtration;
Described ultrafiltration step, with the tubular fibre combination ultrafiltration of 4000 molecular weight.
The production method of low bacterium height motility rate papoid above, the advantage that the hollow fiber conduit that microfiltration step is divided into three groups gradually little sequentially filters is: filter fast, easy cleaning, through detecting, microorganism drops to≤and 100; Ultrafiltration step adopts the hollow fiber conduit combination ultrafiltration of 4000 molecular weight, does not substantially run leakage.After improving method, papoid production cost is declined to some extent, improves and extract the quality of production of papoid and output from papaya milk.
Embodiment
According to the technological process of production, collect and check the papaya milk of Papain Enzymatic Activity u/g >=120,000; To collect and papaya milk after inspection is put into colloidal mill and carried out emulsification fineness, the papaya milk after emulsification fineness is inserted disc separator carries out water, impurity is separated with fiber, obtain moisture papoid.Moisture papoid is carried out conventional refrigeration, in tubular fibre combination Microfilter to be entered, carries out micro-filtration.The tubular fibre combination Microfilter model of former technique is MFOa, 0.2 μm of hollow fiber conduit, working pressure≤0.08Mpa, main elimination microbial bacterial.On the basis of former tubular fibre combination Microfilter, improve and sequentially with three groups of hollow fiber conduits, micro-filtration carried out to papaya milk: first group with 0.4 μm of hollow fiber conduit filter, second group with 0.2 μm of hollow fiber conduit filtration, the 3rd group with 0.1 μm of hollow fiber conduit filtration.Papoid through three groups of hollow fiber conduit micro-filtrations is detected, microorganism drops to≤and 100, substantially increase papoid quality; And carry out compared with micro-filtration with former method 0.2 μm of hollow fiber conduit, also find to have filtered fast, pipeline also easily cleaned, improve productivity.To the papoid after three groups of hollow fiber conduit micro-filtrations, carry out ultrafiltration with the tubular fibre combination of 4000 molecular weight, substantially do not run leakage, add output.Processing step backward: lyophilize → pulverizing → packaging, carrying out routinely.
After using the production method of a kind of low bacterium papoid of the present invention, papoid production cost declines to some extent, and productivity, Quality and yield improve greatly.
Claims (1)
1. a production method for low bacterium height motility rate papoid, it takes full advantage of papaya milk, comprises micro-filtration and ultrafiltration step, it is characterized in that:
Described microfiltration step, sequentially with three groups of hollow fiber conduits, micro-filtration is carried out to papaya milk: first group with 0.4 μm of hollow fiber conduit filter, second group with 0.2 μm of hollow fiber conduit filter, the 3rd group with 0.1 μm of hollow fiber conduit filtration;
Described ultrafiltration step, with the tubular fibre combination ultrafiltration of 4000 molecular weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310479567.7A CN103509774B (en) | 2013-10-15 | 2013-10-15 | Manufacturing method of low-bacterium papain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310479567.7A CN103509774B (en) | 2013-10-15 | 2013-10-15 | Manufacturing method of low-bacterium papain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103509774A CN103509774A (en) | 2014-01-15 |
| CN103509774B true CN103509774B (en) | 2015-07-01 |
Family
ID=49893209
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310479567.7A Expired - Fee Related CN103509774B (en) | 2013-10-15 | 2013-10-15 | Manufacturing method of low-bacterium papain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103509774B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109321554B (en) * | 2018-11-30 | 2021-11-09 | 广西南宁天绿生物制品有限公司 | Production method of special papain for beer |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101096639B (en) * | 2007-05-16 | 2011-08-10 | 宁波 | Multi-group fiber pipes even distributed multifunctional hollow fiber film pipe bioreactor |
| CN101570748A (en) * | 2008-05-04 | 2009-11-04 | 徐闻县美仑生物制品有限公司 | Method for refining papain |
| CN202860415U (en) * | 2012-10-26 | 2013-04-10 | 中国石油化工股份有限公司 | Fiber ethanol fermentation broth microfiltration assembly |
| CN103315128B (en) * | 2013-05-29 | 2014-12-17 | 中国农业科学院农产品加工研究所 | Casein powder and preparation method thereof |
-
2013
- 2013-10-15 CN CN201310479567.7A patent/CN103509774B/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN103509774A (en) | 2014-01-15 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150701 |
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