CN103503988A - Method for treating and preventing Huangguan pear Jizhua disease by using calcium chloride - Google Patents

Method for treating and preventing Huangguan pear Jizhua disease by using calcium chloride Download PDF

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Publication number
CN103503988A
CN103503988A CN201310467395.1A CN201310467395A CN103503988A CN 103503988 A CN103503988 A CN 103503988A CN 201310467395 A CN201310467395 A CN 201310467395A CN 103503988 A CN103503988 A CN 103503988A
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China
Prior art keywords
huangguan pear
calcium chloride
disease
jizhua
content
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CN201310467395.1A
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Chinese (zh)
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寇晓虹
吴梦诗
李磊
薛照辉
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Tianjin University
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Tianjin University
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Abstract

The invention discloses a method for treating and preventing Huangguan pear Jizhua disease by using calcium chloride. The method comprises the following steps: Huangguan pear fruits are dipped in a calcium chloride water solution with mass concentration of 1-3% for 13-18 minutes, then are taken out, drained off, placed for 8-12 hours and packed, and are put in a refrigeratory for storage. The systematic study on some physiological and biochemical characteristic changes in the stored harvested fruit and the Jizhua disease morbidity is performed, and more importantly, the more systematic study on the influences on the alpha-farnesene and conjugated triene content in Huangguan pear peels, on the phenolic substance content, on the content of different forms of calcium in the peels and the Jizhua disease morbidity and the like, caused by different treatment, in the storage period is performed, and the result shows that the method disclosed by the invention can effectively prevent the Huangguan pear Jizhua disease, and the effect is obviously better than that of a control group. The novel method is based on the pathogeny of the Jizhua disease. The method disclosed by the invention has the advantages that the process for preventing the Huangguan pear Jizhua disease is simple and the effect is remarkable.

Description

With calcium chloride, process the method for control Huangguan Pear jizhua disease
Technical field
The invention belongs to the prevention and control field of postharvest fruit and vegetable physiological maladies, relate to the method for integrated control of Huangguan Pear jizhua disease.
Background technology
Huangguan Pear belongs to white pear system, that Hebei province fruit tree research institute is by Chinese pear and Japan's new century pears cross breeding, maturity period is mid-August, belong to mid-early maturity pears breeding, compared with about 30 days morning such as pear, Chinese pears, there is the good characteristics such as morning in maturity period, good looking appearance, best in quality, scab resistant, obtained the domestic and international producer, consumer's generally approval.The ground large area such as current Yi Hebei, Shandong, Henan and Liaoning are planted, and Hebei province's plantation just has 200,000 mu.Huangguan Pear fruit ovalize, single fruit weight 200~330g, ripening fruits fruit yellow, fruit dot is little, and pulp is pure white, and meat is fine and smooth, lithocyte and residue are few, crisp succulence, local flavor sour-sweet is agreeable to the taste, and has strong fragrance, core is little, quality is superior, liked by consumers in general, and development prospect is very wide.
In recent years, there is a kind of disease in Huangguan Pear, and the speckle of brown appears in fruit surface, likeness in form shank, and orchard worker is referred to as " shank is sick ".Huangguan Pear jizhua disease all can be fallen ill in field and storage transportation, field morbidity, and general time diseased fruit rate 10%~30%, serious can be more than 90%; In storage transportation, the incidence of disease is about 30%, and serious can, more than 90%, cause massive losses to orchard worker and dealer.
Calcium physiological action within plant tissue comprises two aspects, and calcium can maintain the ability of cell synthetic protein on the one hand, participates in the formation of cell membrane, keeps plasmalemma structure and stable performance, maintains the integrality of body; On the other hand, the biosynthesis of calcium energy ethene suppressing, postpones fruit After-ripening, and delayed fruit is old and feeble.Endogenous calcium content in fruit is not enough or and other element between balance be broken, easily cause various physiological diseases.The physiological disease causing for fruit calcium deficiency, if soak calcium measure after taking, the calcium content in fruit can be enhanced in various degree, can suppress the physiological disease that fruit calcium deficiency causes, as pears dehiscent fruit, pears black heart etc., but the method is not applied to the control of Huangguan Pear jizhua disease at present.
Summary of the invention
The present invention seeks to overcome the deficiencies in the prior art, a kind of method of processing control Huangguan Pear jizhua disease with calcium chloride is provided.
Technical scheme of the present invention is summarized as follows:
The method of processing control Huangguan Pear jizhua disease with calcium chloride, comprises the steps: Huangguan Pear fruit to be soaked in 13-18min in the calcium chloride water that mass concentration is 1%-3%, takes out, and drains, and places 8-12h, puts into freezer and preserve after packing.
The mass concentration of described calcium chloride water is preferably 2%.
By some physiological and biochemical properties variations in fruit postharvest storage and the sick incidence of shank, carry out systematic research, emphatically at duration of storage, different disposal on the impact of different shape calcium content in α-farnesene in Huangguan Pear pericarp and conjugated triene content influence, aldehydes matter content impact, pericarp, comparatively system research is carried out in the impacts of the rate of disease etc., confirm that method of the present invention can effectively prevent the generation of Huangguan Pear jizhua disease, is obviously better than control group.Method of the present invention is based upon on the sick pathogenesis of shank basis.Method of the present invention is simple to the process of preventing and treating of Huangguan Pear jizhua disease, and effect is remarkable.
Accompanying drawing explanation
Fig. 1 blank and the storage of the method for the present invention processing Huangguan Pear sick incidence of shank in the time of 5 months.
Fig. 2 processes the impact on aldehydes matter content in Huangguan Pear duration of storage pericarp by method of the present invention.
Fig. 3 processes the impact on PPO, POD, CAT, SOD activity in Huangguan Pear duration of storage pericarp by method of the present invention.
Fig. 4 processes the impact on MDA content in Huangguan Pear duration of storage pericarp by method of the present invention.
Fig. 5 processes the impact on α-farnesene, conjugated triene content in Huangguan Pear duration of storage different tissues by method of the present invention.
Fig. 6 processes the impact on the full calcium of Huangguan Pear pericarp by method of the present invention.
Fig. 7 processes the impact on Huangguan Pear duration of storage pericarp different calcium form content by method of the present invention.
Fig. 8 processes the impact that Huangguan Pear duration of storage pericarp is shown to institutional framework by method of the present invention.
Fig. 9 processes the impact on Huangguan Pear duration of storage pericarp fractography structure by method of the present invention.
The specific embodiment
Embodiments of the invention are in order to enable those skilled in the art to understand better the present invention, can not to impose any restrictions the present invention.
Below by specific embodiment, the present invention is further illustrated.
Embodiment 1
With calcium chloride, process the method for control Huangguan Pear jizhua disease, comprise the steps: that it is 15min in 2% calcium chloride water that Huangguan Pear fruit is soaked in to mass concentration, take out, drain, place 10h and distribute field heat, after packing, put into 0 ℃ of storage of freezer.
When the method processing Huangguan Pear of blank and the present embodiment is preserved to 5 months, the sick incidence of shank is observed and is seen Fig. 1.In figure: A is blank, does not carry out any processing, B adopts the method for the present embodiment to process.
By the method for the embodiment of the present invention 1, process the impact of aldehydes matter content in Huangguan Pear duration of storage pericarp is shown in to Fig. 2.In figure: A: chlorogenic acid; B. ursin; C. catechin; D. caffeic acid; E. coumaric acid; F. Quercetin.
By the method for the embodiment of the present invention 1, process the impact of PPO, POD, CAT, SOD activity in Huangguan Pear duration of storage pericarp is shown in to Fig. 3.
By the method for the embodiment of the present invention 1, process the impact of MDA content in Huangguan Pear duration of storage pericarp is shown in to Fig. 4.
By the method for the embodiment of the present invention 1, process the impact of α-farnesene, conjugated triene content in Huangguan Pear duration of storage different tissues is shown in to Fig. 5.In figure: the content of α-farnesene in A. pericarp; B. the content of α-farnesene in wax coat; C. the content of α-farnesene in cuticula; D. the content of α-farnesene in nearly fruit pulp cell; E. the content of α-farnesene in pulp; F. the content of α-farnesene in nearly fruit stone pulp; G. the content of conjugated triene in pericarp; H. the content of conjugated triene in wax coat; I. the content of conjugated triene in cuticula; J. the content of conjugated triene in nearly fruit pulp cell; K. the content of conjugated triene in pulp; L. the content of conjugated triene in nearly fruit stone pulp.
By the method for the embodiment of the present invention 1, process the impact of the full calcium of Huangguan Pear pericarp is shown in to Fig. 6.
By the method for the embodiment of the present invention 1, process the impact of Huangguan Pear duration of storage pericarp different calcium form content is shown in to Fig. 7.
By the method for the embodiment of the present invention 1, process Huangguan Pear duration of storage pericarp is shown to the impact of institutional framework is shown in Fig. 8.
In figure: A. storage pericarp surface texture in early stage, amplifies 200 times under Electronic Speculum; B. storage pericarp surface texture in early stage, amplifies 1500 times under Electronic Speculum; C. the sick site of pathological change pericarp of shank surface texture, amplifies 200 times under Electronic Speculum; D. the sick site of pathological change pericarp of shank surface texture, amplifies 1500 times under Electronic Speculum; E. the sick site of pathological change surface horny layer of shank structure, amplifies 200 times under Electronic Speculum; F. the sick site of pathological change surface horny layer of shank structure, amplifies 200 times under Electronic Speculum; G. preserve and contrast the pericarp surface texture of not falling ill latter stage, under Electronic Speculum, amplify 200 times; H. contrast the pericarp surface texture of not falling ill latter stage, under Electronic Speculum, amplify 1500 times; J. preserve and soak calcium processing pericarp surface texture, 200 times of the large amplifications of Electronic Speculum latter stage.
By the method for the embodiment of the present invention 1, process the impact of Huangguan Pear duration of storage pericarp fractography structure is shown in to Fig. 9.In figure: A. storage pericarp section structure in early stage, amplifies 100 times under Electronic Speculum; B. storage pericarp section structure in early stage, amplifies 500 times under Electronic Speculum; C. the sick site of pathological change pericarp of shank section structure, amplifies 100 times under Electronic Speculum; D. the sick site of pathological change pericarp of shank section structure, amplifies 500 times under Electronic Speculum; E. preserve and contrast the pericarp section structure of not falling ill latter stage, under Electronic Speculum, amplify 100 times; F. preserve and soak calcium processing pericarp section structure latter stage, under Electronic Speculum, amplify 100 times.
Embodiment 2
With calcium chloride, process the method for control Huangguan Pear jizhua disease, comprise the steps: that it is 18min in 1% calcium chloride water that Huangguan Pear fruit is soaked in to mass concentration, take out, drain, place 12h and distribute field heat, after packing, put into 0 ℃ of storage of freezer.Embodiment 3
With calcium chloride, process the method for control Huangguan Pear jizhua disease, comprise the steps: that it is 13min in 3% calcium chloride water that Huangguan Pear fruit is soaked in to mass concentration, take out, drain, place 8h and distribute field heat, after packing, put into 0 ℃ of storage of freezer.
After the method that experiment showed, embodiment 2 and embodiment 3 is processed Huangguan Pear, its effect is similar to embodiment 1.

Claims (2)

1. with calcium chloride, process the method for control Huangguan Pear jizhua disease, it is characterized in that comprising the steps: Huangguan Pear fruit to be soaked in 13-18min in the calcium chloride water that mass concentration is 1%-3%, take out, drain, place 8-12h, after packing, put into freezer and preserve.
2. the method for processing control Huangguan Pear jizhua disease with calcium chloride according to claim 1, the mass concentration that it is characterized in that described calcium chloride water is 2%.
CN201310467395.1A 2013-09-30 2013-09-30 Method for treating and preventing Huangguan pear Jizhua disease by using calcium chloride Pending CN103503988A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105941611A (en) * 2016-02-03 2016-09-21 山东营养源食品科技有限公司 Method for supplementing fruits and vegetables with calcium after harvesting
CN107056450A (en) * 2017-04-20 2017-08-18 山东省果树研究所 Combination strain fertilizer and its application for preventing and treating pears Jizhua disease
CN109042862A (en) * 2018-07-26 2018-12-21 无为县辉琴农副产品销售部 Improve the fresh-keeping and preserving method of the operatic circle condition

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
李磊: "黄冠梨鸡爪病发病机理及调控技术研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105941611A (en) * 2016-02-03 2016-09-21 山东营养源食品科技有限公司 Method for supplementing fruits and vegetables with calcium after harvesting
CN107056450A (en) * 2017-04-20 2017-08-18 山东省果树研究所 Combination strain fertilizer and its application for preventing and treating pears Jizhua disease
CN109042862A (en) * 2018-07-26 2018-12-21 无为县辉琴农副产品销售部 Improve the fresh-keeping and preserving method of the operatic circle condition

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Application publication date: 20140115