CN103499624B - 一种农药残留检测仪生物传感器试剂包的制作方法 - Google Patents
一种农药残留检测仪生物传感器试剂包的制作方法 Download PDFInfo
- Publication number
- CN103499624B CN103499624B CN201310494683.6A CN201310494683A CN103499624B CN 103499624 B CN103499624 B CN 103499624B CN 201310494683 A CN201310494683 A CN 201310494683A CN 103499624 B CN103499624 B CN 103499624B
- Authority
- CN
- China
- Prior art keywords
- pack
- solution
- instrument
- detecting pesticide
- biology sensor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 15
- 239000000447 pesticide residue Substances 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 claims abstract description 11
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000000575 pesticide Substances 0.000 claims description 10
- 108010022752 Acetylcholinesterase Proteins 0.000 claims description 9
- 229940022698 acetylcholinesterase Drugs 0.000 claims description 8
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 claims description 4
- 229960001231 choline Drugs 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 230000004048 modification Effects 0.000 claims description 3
- 238000012986 modification Methods 0.000 claims description 3
- 239000002048 multi walled nanotube Substances 0.000 claims description 3
- 229920000557 Nafion® Polymers 0.000 claims description 2
- 125000002668 chloroacetyl group Chemical group ClCC(=O)* 0.000 claims description 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 2
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 2
- 235000019800 disodium phosphate Nutrition 0.000 claims description 2
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 2
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 2
- 238000005987 sulfurization reaction Methods 0.000 claims description 2
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 2
- 239000012498 ultrapure water Substances 0.000 claims description 2
- 102000012440 Acetylcholinesterase Human genes 0.000 claims 1
- 239000006185 dispersion Substances 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- QWJYDTCSUDMGSU-UHFFFAOYSA-N [Sn].[C] Chemical compound [Sn].[C] QWJYDTCSUDMGSU-UHFFFAOYSA-N 0.000 abstract description 4
- 150000001875 compounds Chemical class 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 3
- 229920001661 Chitosan Polymers 0.000 abstract description 3
- 229910052799 carbon Inorganic materials 0.000 abstract description 3
- 238000012360 testing method Methods 0.000 abstract description 3
- 239000002114 nanocomposite Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 239000002131 composite material Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 description 16
- 108090000790 Enzymes Proteins 0.000 description 16
- 229940088598 enzyme Drugs 0.000 description 16
- 102100033639 Acetylcholinesterase Human genes 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- 239000003987 organophosphate pesticide Substances 0.000 description 5
- 235000013311 vegetables Nutrition 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 4
- GFFIJCYHQYHUHB-UHFFFAOYSA-N 2-acetylsulfanylethyl(trimethyl)azanium Chemical compound CC(=O)SCC[N+](C)(C)C GFFIJCYHQYHUHB-UHFFFAOYSA-N 0.000 description 3
- 239000005944 Chlorpyrifos Substances 0.000 description 3
- 208000005374 Poisoning Diseases 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- SBPBAQFWLVIOKP-UHFFFAOYSA-N chlorpyrifos Chemical compound CCOP(=S)(OCC)OC1=NC(Cl)=C(Cl)C=C1Cl SBPBAQFWLVIOKP-UHFFFAOYSA-N 0.000 description 3
- 238000004817 gas chromatography Methods 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 231100000572 poisoning Toxicity 0.000 description 3
- 230000000607 poisoning effect Effects 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 239000003905 agrochemical Substances 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 238000005251 capillar electrophoresis Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 102000003914 Cholinesterases Human genes 0.000 description 1
- 108090000322 Cholinesterases Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 231100000570 acute poisoning Toxicity 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940048961 cholinesterase Drugs 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明涉及一种农药残留检测仪生物传感器试剂包的制作方法，属于生物传感器制备领域。本发明给出了专门用于农药残留检测仪的试剂包。第一步为试剂包的制备，说明书中给出了详细的制备方法。第二步为多壁碳-二氧化锡-壳聚糖纳米复合材料的制备：以多壁碳、二氧化锡、壳聚糖为原料，利用多壁碳-二氧化锡-壳聚糖复合材料的良好电化学氧化还原活性、好的生物相溶性以及高度的化学稳定性，修饰工作电极以促进电子转移。本发明的试剂包，配制方法简单，测试结果准确，灵敏度高，稳定性好。
Description
本发明提供农药残留检测仪生物传感器试剂包的制作方法，属于生物传感器制备领域。
背景技术
近年来，农药残留引起的中毒事件屡屡发生，果蔬中的有害、剧毒农药的残留问题一直是人们关注的焦点，也是现今中国加入WTO 后，农产品出口遭遇“绿色壁垒”的主要影响因素之一。随着我国人民生活水平不断提高，农产品的质量安全问题越来越受到关注，尤其果品、蔬菜中农药残留问题已经成为公众关注的焦点。全国每年都有上百起因食用被农药污染的农产品而引起的急性中毒事件，严重影响广大消费者的身体健康，因此由农药残留超标导致的食品安全问题，已越来越受到各国政府和公众的重视。有机磷农药是我国应用最为广泛的一类农药，其中毒死蜱是目前全世界生产和销售量最大的杀虫剂品种之一。毒死蜱是高效中毒的广谱杀虫剂兼除草剂，具有一定的内吸性，可以通过食物链的富集作用转移至人体，对人体具有潜在致癌作用。可见，加强对农产品中农药残留的检测对保护生态环境，尤其是保障人类健康有着十分深远的意义，其中毒死蜱一直是环境和食品中农药残留检测的重要项目。
传统的农药残留检测方法主要有：气相色谱（GC）、高效液相色谱法(HPLC)、色谱/质谱联用技术(GC/LC-MS)、毛细管电泳法(CE)、荧光分析、酶联免疫法(ELISA)。这些方法虽然选择性好、灵敏度高、准确度高、检出限低，可同时检测多种元素或化合物，但其需要昂贵的仪器设备，样品前处理过程繁琐、费时，并且对分析人员的技术水平要求很高，不适于现场快速检测。常用的农药残留快速检测方法有酶抑制法( 酶抑制试纸法和酶抑制分光光度法)，可以实现有机磷农药的现场快速检测，具有较好的实用价值。但是，速测卡是通过肉眼观察卡片的颜色变化，因此一般只能用于严重超标的蔬菜样品的定性测量。酶抑制分光光度法的原理是基于吸光度的变化进行检测的，但蔬菜水果中大量的色素会影响检测结果的准确度。并且上述方法存在回收率低、错检、漏检比例较高、重复性差、难以满足低残留和定量检测的要求等缺点。酶传感器是利用有机磷的毒理性原理所研制的传感器，与传统的分析方法相比，它具有特异性强、分析速度快、结构简单、成本低廉等优点。
发明的目的在于提供一种能克服上述缺陷以及操作简单、灵敏度高、检出限高的检测毒死蜱农药残留的酶传感器试剂包的制备方法。
其技术方案为：一种农药残留检测仪生物传感器试剂包的制作方法，其特征在于：操作简单；敏感界面组成为由多壁碳、二氧化锡、壳聚糖制备的纳米复合膜，进而进行酶固定技术，灵敏度高。
其制备原理为：以乙酰胆碱酯酶的催化性为基础的酶电化学传感器是将乙酰胆碱酯酶固定在金电极表面，催化底物乙酰硫代胆碱（ATCl）水解，产生具有电活性的巯基胆碱，巯基胆碱可在金电极表面发生氧化反应，产生的电信号可转化为电流，在一定条件下该电流的大小可反映乙酰胆碱酯酶的活性。由于乙酰胆碱酯酶是一种以三个丝氨酸残基联体为活性的酶，当其中的任何一个氨基酸残基遭到破坏时，将使酶丧失催化活性。有机磷农药与乙酰胆碱酯酶作用能形成稳定的磷酰化胆碱酯酶而破坏其氨基酸残基结构，从而使乙酰胆碱酯酶失去活性，降低催化乙酰硫代胆碱水解的能力。因此，在加入含有机磷农药的底物溶液中，因部分酶活性被抑制导致其相应电流减小。通过比较酶被抑制前后的电流信号的大小，可对有机磷农药进行定性检测；本发明采用以壳聚糖作为分散剂制备的多壁碳- 二氧化锡复合物对金电极进行修饰。多壁碳纳米管(MWCNTs) 拥有独特的电化学特性、较高的化学稳定性和机械强度，被广泛用于酶传感器敏感界面的制作。壳聚糖(CHIT) 属于多糖类，它具有优异的成膜性、吸附性、透气性和渗透性，成膜后具有很好的吸附性、稳定性和良好的生物相容性，其丰富的氨基、多孔性结构使它被广泛用于生物分子的固定和修饰电极的制备。壳聚糖的氨基端连接二氧化锡，为酶在电极表面的固定提供了一个良好的生物界面，保持了酶的生物活性以用来和农药进行抑制反应。此外，二氧化锡具有较高的比表面积、稳定性和导电性可以增大电极表面电子的传导能力和生物相容性，进而增大酶传感器的响应电流。采用本发明制成的电流型酶传感器可以在蔬果采收、上市前，进行农药残留的快速测定，直接对农药残留量是否超标进行检测，避免因食用含有残留农药的蔬果而引起中毒，为农产品安全生产与消费提供残留检测的技术支撑。
本发明以具有良好的生物相容性和成膜性的壳聚糖作为分散剂分散多壁碳和二氧化锡所制备成的多壁碳- 二氧化锡- 壳聚糖纳米复合物膜能够促进电化学反应中电子的传递，提高电极上的响应电流，改善电极表面的微环境，因而可以作为载体材料，用以制备响应信号强和灵敏度高的酶传感器。
具体实施方式
1、试剂包的配制方法
试剂包一：磷酸二氢钠0.3839g，磷酸氢二钠2.3856g，氯代乙酰硫化胆碱0.0395g ；
将试剂包一内的固体全部倒入200ml 塑料滴瓶中，然后加入200ml 超纯水，使之溶解并摇匀，称为溶液一；
试剂包二：0.01g 壳聚糖；
试剂瓶三：10ml 乙酸溶液；
将试剂包二内的固体全部倒入试剂瓶三中，混合均匀，称为溶液二；
试剂包四：2.5mg 二氧化锡，7.5mg 多壁碳纳米管；
将试剂包四内粉末倒入溶液二中，混合均匀，称为溶液三（MWCNTS-SNO2-CHIT）；
溶液四：0.02U/μL 乙酰胆碱酯酶；
溶液五：0.5% Nafion 溶液；
2、电极的修饰
溶液四必须在冰箱0~4℃中保存；进行农药残留检测时在裸电极表面滴加溶液三7.5μL ；待表面修饰物干后，滴加溶液四7.5μL，干后滴加2.5μL溶液五完成乙酰胆碱酯酶生物传感器的制备，在0~4℃中保存待检测使用。
Claims (2)
1.一种农药残留检测仪生物传感器试剂包的制作方法，其特征在于：制备步骤为：试剂包一：磷酸二氢钠0.3839g，磷酸氢二钠2.3856g，氯代乙酰硫化胆碱0.0395g ；将试剂包一内的固体全部倒入200ml 塑料滴瓶中，然后加入200ml 超纯水，使之溶解并摇匀，称为溶液一；
试剂包二：0.01g 壳聚糖；
试剂瓶三：10ml 乙酸溶液；
将试剂包二内的固体全部倒入试剂瓶三中，混合均匀，称为溶液二；
试剂包四：2.5mg 二氧化锡，7.5mg 多壁碳纳米管；
将试剂包四内粉末倒入溶液二中，混合均匀，称为溶液三MWCNTS-SnO2-CHIT 分散液；
溶液四：0.02U/μL 乙酰胆碱酯酶；
溶液五：0.5% Nafion 溶液。
2.如权利要求1 所述的一种农药残留检测仪生物传感器试剂包的制作方法，其特征在于：溶液四必须在冰箱0~4℃中保存；进行农药残留检测时在裸电极表面滴加溶液三7.5μL ；待表面修饰物干后，滴加溶液四7.5μL，干后滴加2.5μL溶液五完成乙酰胆碱酯酶生物传感器的制备，在0~4℃中保存待检测使用。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310494683.6A CN103499624B (zh) | 2013-10-18 | 2013-10-18 | 一种农药残留检测仪生物传感器试剂包的制作方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310494683.6A CN103499624B (zh) | 2013-10-18 | 2013-10-18 | 一种农药残留检测仪生物传感器试剂包的制作方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103499624A CN103499624A (zh) | 2014-01-08 |
| CN103499624B true CN103499624B (zh) | 2015-11-04 |
Family
ID=49864852
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310494683.6A Expired - Fee Related CN103499624B (zh) | 2013-10-18 | 2013-10-18 | 一种农药残留检测仪生物传感器试剂包的制作方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103499624B (zh) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223251A (zh) * | 2015-10-19 | 2016-01-06 | 山东理工大学 | 一种基于丝网印刷电极检测有机磷农药的传感器的制备方法及应用 |
| CN110455885A (zh) * | 2019-09-17 | 2019-11-15 | 东北农业大学 | 一种通过电化学双酶传感器检测毛油中pc含量的方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102103115A (zh) * | 2011-01-26 | 2011-06-22 | 山东理工大学 | 一种电化学乙酰胆碱酯酶生物传感器的制备方法 |
| CN102645460A (zh) * | 2012-03-31 | 2012-08-22 | 无锡百灵传感技术有限公司 | 一种对齐墩果酸有特定识别能力的印迹电化学传感器制备方法 |
-
2013
- 2013-10-18 CN CN201310494683.6A patent/CN103499624B/zh not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102103115A (zh) * | 2011-01-26 | 2011-06-22 | 山东理工大学 | 一种电化学乙酰胆碱酯酶生物传感器的制备方法 |
| CN102645460A (zh) * | 2012-03-31 | 2012-08-22 | 无锡百灵传感技术有限公司 | 一种对齐墩果酸有特定识别能力的印迹电化学传感器制备方法 |
Non-Patent Citations (3)
| Title |
|---|
| Acetylcholinesterasebiosensorbasedonchitosan/prussianblue/multiwall carbon nanotubes/hollow gold nanospheres nanocomposite film by one-step electrodeposition;Chen Zhai等;《Biosensors and Bioelectronics》;20121029;第124-130页 * |
| Determination of carbaryl pesticide using amperometric acetylcholinesterase sensor formed by electrochemically deposited chitosan;Dan Du等;《Colloids and Surfaces B》;20070313;第145-150页 * |
| Electrochemical impedimetric DNA sensing based on multi-walled carbon nanotubes-SnO2-chitosan nanocomposite;Tao Yang等;《Colloids and Surfaces B:Biointerfaces》;20130130;第257-261页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103499624A (zh) | 2014-01-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| He et al. | Research on the construction of portable electrochemical sensors for environmental compounds quality monitoring | |
| Bilal et al. | An insect acetylcholinesterase biosensor utilizing WO3/g-C3N4 nanocomposite modified pencil graphite electrode for phosmet detection in stored grains | |
| Zhang et al. | Portable glucose meter: trends in techniques and its potential application in analysis | |
| Pisoschi et al. | Electrochemical methods for total antioxidant capacity and its main contributors determination: A review | |
| Umapathi et al. | Sowing kernels for food safety: Importance of rapid on‐site detction of pesticide residues in agricultural foods | |
| Oliveira et al. | Biosensor based on multi-walled carbon nanotubes paste electrode modified with laccase for pirimicarb pesticide quantification | |
| Lu et al. | Nitrophenylboronic acids as highly chemoselective probes to detect hydrogen peroxide in foods and agricultural products | |
| Valdés-Ramírez et al. | Sensitive amperometric biosensor for dichlorovos quantification: Application to detection of residues on apple skin | |
| Si et al. | Wiring bacterial electron flow for sensitive whole-cell amperometric detection of riboflavin | |
| Johnson et al. | Electrochemical sensing of neonicotinoids using laser-induced graphene | |
| Nadar et al. | Enzyme embedded microfluidic paper-based analytic device (μPAD): a comprehensive review | |
| Mohiuddin et al. | Alpha-glucosidase enzyme biosensor for the electrochemical measurement of antidiabetic potential of medicinal plants | |
| Li et al. | Ratiometric fluorescent hydrogel for point-of-care monitoring of organophosphorus pesticide degradation | |
| CN103499619B (zh) | 一种检测有机磷农药的乙酰胆碱酯酶传感器制备方法 | |
| CN105021679A (zh) | 一种基于离子液体-多壁碳纳米管修饰的丝网印刷电极的传感器制备方法 | |
| Hossain et al. | Amperometric Detection of Parathion and Methyl Parathion with a Microhole‐ITIES | |
| CN104634843A (zh) | 一种基于丝网印刷碳电极的传感器的制备方法及应用 | |
| Zhang et al. | Sensitive detection of polynucleotide kinase activity by paper-based fluorescence assay with λ exonuclease assistance | |
| CN104931554A (zh) | 一种基于丝网印刷碳电极的传感器制备方法及应用 | |
| CN103630587B (zh) | 一种快速、灵敏检测有机磷农药毒死蜱的方法 | |
| Ye et al. | Construction of an immobilised acetylcholinesterase column and its application in screening insecticidal constituents from Magnolia officinalis | |
| CN103558269B (zh) | 一种检测毒死蜱农药残留的酶传感器制备方法 | |
| Che et al. | Fluorescent and colorimetric ionic probe based on fluorescein for the rapid and on-site detection of paraquat in vegetables and the environment | |
| Wen et al. | Complementary Homogeneous Electrochemical and Photothermal Dual-Modal Sensor for Highly Sensitive Detection of Organophosphorus Pesticides via Stimuli-Responsive COF/Methylene Blue@ MnO2 Composite | |
| Huang et al. | Cubic Ag2O nanoparticles as robust laccase mimetics in a smartphone-assisted colorimetric sensor for rapid and ultrasensitive detection of kanamycin in environment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20151104 |