CN103499299B - A kind of very thin thread measurement of length method - Google Patents
A kind of very thin thread measurement of length method Download PDFInfo
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- CN103499299B CN103499299B CN201310506144.XA CN201310506144A CN103499299B CN 103499299 B CN103499299 B CN 103499299B CN 201310506144 A CN201310506144 A CN 201310506144A CN 103499299 B CN103499299 B CN 103499299B
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- double dish
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Abstract
The present invention discloses a kind of very thin thread measurement of length method, and the technical scheme adopted is: the glass culture dish that setting two penetrabilitys are higher, viscose paper prints and makes grid viscose paper; With adsorbent, grid viscose paper is adsorbed in upper double dish, in lower double dish, drips one deck float supporting agent, the dispersion of very thin thread is placed in lower double dish make between each thread not overlapping; Be positioned in lower double dish by upper double dish, the total intersection adding up very thin thread and the grid on grid viscose paper line anyhow counts; The right-angled intersection method formula R=11/14 × N × a improved is utilized to converse the length of very thin thread, the present invention uses the viscose paper that can print, in conjunction with concrete measurement thing condition design grid size, adopt the upper and lower double dish structure of the clamping plate type of two-layer transparent glass simultaneously, make very thin thread to be measured be not easy to float in carrying agent, thus make measurement result more accurate.Use simple, feasibility is strong, and accuracy is high.
Description
Technical field
The present invention relates to a kind of measuring method, specifically a kind of very thin thread measurement of length method, belongs to field of measuring technique.
Background technology
Measuring the tiny root system of plant and the mycelia equal diameter thread length at random at below 1mm, is the important content that researching plant root and hypogeal fungus make aspect mutually.And root scanner device traditional is at present measured accurate not for this type of thread.In order to measure the thread length of below this type of diameter, people have only and rely on traditional right-angled intersection method (author: Newman, E " Amethodofestimatingthetotallengthofrootinasample " " JournalofappliedEcology " 139-145, 1966), and the length utilizing the right-angled intersection method formula R=11/14 × N × a improved to converse very thin thread is measured, R is the length measuring thing, N counts all intersection of thread and the grid measured line anyhow, a is the lattice length of side (author: Tennant, D " Atestofamodifiedlineintersectmethodofestimatingrootlengt h " " TheJournalofEcology ", 1975).Right-angled intersection method is measured to the length of this type of thread, need to rely on the container that wire material is measured in the enlargement factor of stereomicroscope, the size of grid and carrying.For the thread of such diameter, current people adopt the container of different band different size grids for different-diameter and the different thread measured, and many bottoms grid being prepared in container.In addition, go back multipurpose liquid to launch by thread as suspending agent.Like this wire material measuring different amount and different-diameter is seemed and extremely bother, and in the process measured under Stereo microscope, rock and the wire material in suspending agent can be made to move about, thus affect the accuracy of measurement result.Still there is no a kind of very thin thread measurement of length method being difficult to resolution for measuring naked eyes of simple many specifications at present.
Summary of the invention
The object of the invention is to overcome the defect existing for measuring very thin thread at random at present, and disclose a kind of very thin thread measurement of length method, thus make measurement result more accurate.
The technical solution adopted for the present invention to solve the technical problems is:
A. set the higher glass culture dish of two penetrabilitys, the bottom thickness wherein going up double dish is thinner, and the interior diameter of lower double dish is greater than the overall diameter of double dish;
B. designing size dimension is the lattice of 28/11-7/22cm, and adopts the thinnest linear grid to be printed on viscose paper by printer, makes grid viscose paper;
C. in upper double dish, drip the high vegetable oil adsorbent of layer of transparent degree, have the grid viscose paper of design size to be placed in upper double dish printing, grid viscose paper to be adsorbed in double dish and not to stay bubble with bottom;
D. in lower double dish, drip that one deck viscosity is high, the floating supporting agent of good, the good water solubility of the transparency, can under the enlargement factor of clear identification, very thin thread dispersion is placed in lower double dish at Stereo microscope, not overlapping between each thread;
E. then the upper double dish with grid viscose paper is positioned in lower double dish, in put procedure, does not occur bubble;
F. double dish and upper double dish is descended to rely on the floating supporting agent in middle layer to adsorb, thus paste together, then, under being placed on Stereo microscope, count in total intersection the can adding up very thin thread and the cross grid on grid viscose paper line anyhow under the enlargement factor of clear identification;
G. utilize the right-angled intersection method formula R=11/14 × N × a of improvement to converse the length of very thin thread, wherein R measures the length of thing, and to be the thread measured count with all intersection of grid line anyhow N, and a is the lattice length of side.
The present invention is owing to using the viscose paper that can print, design grid size is carried out in conjunction with concrete measurement thing condition, adopt the upper and lower double dish structure of the clamping plate type of two-layer transparent glass simultaneously, make very thin thread to be measured be not easy to float in carrying agent, thus make measurement result more accurate.Device involved in the present invention all can reach under general laboratory condition, and use simple, feasibility is strong, and accuracy is high.
Accompanying drawing explanation
Accompanying drawing 1 is concrete operations mode chart of the present invention.
Accompanying drawing 2 is vertical views of accompanying drawing 1 of the present invention.
Diagram: lower double dish 1, upper double dish 2, grid viscose paper 3, vegetable oil adsorbent 4, floating supporting agent 5.
Embodiment
Be described in further details the present invention below in conjunction with specific embodiment and accompanying drawing, following examples are just in order to explain the present invention instead of restriction the present invention:
Embodiment one: utilize the present invention to carry out measuring the root length of below plant 1mm diameter.
A. set the higher glass culture dish of two penetrabilitys, the bottom thickness wherein going up double dish 2 is 0.1cm, and the bottom thickness of lower double dish 1 is 0.15cm, the interior diameter of lower double dish 1 is 14.8cm, overall diameter is 15.2cm, and the interior diameter of upper double dish 2 is 14.2cm, and overall diameter is 14.6cm;
B. collected the root system of plant at random of below diameter 1mm in soil by wet screening, design lattice length is 14/11cm, and utilizes printer to be printed on viscose paper, makes grid viscose paper 3;
C. in upper double dish 2, drip grape-kernel oil adsorbent 4 and evenly launch, then printed grid viscose paper 3 being cut into diameter with scissors is that the circle of 14.1cm is laid in upper double dish 2, grid viscose paper 3 and upper double dish 2 rely on grape-kernel oil adsorbent 4 closely to stick together, and bubble is not stayed in centre;
D. in lower double dish 1, drip skim glycerine and float supporting agent 5, root system at random is placed in its lower double dish 1, under Stereo microscope 20 enlargement factor, root system at random is scatter, make that its each rootlet is intersegmental not to overlap;
E. will be positioned in lower double dish 1 with the upper double dish 2 printing grid viscose paper 3, the surface tension that glycerine floats supporting agent 5 is by lower double dish 1 and upper double dish 2 being attached together tightly, and root system therebetween can not float and not stay bubble;
F. lower double dish 1 is positioned on the objective table of Stereo microscope with the combination unit of upper double dish 2, that under amplification 40 times, to add up on each root segment and grid viscose paper 3 graticule line anyhow intersects sum N, substitute into extent of the root system R=11/14 that formulae discovery is surveyed × N × 14/11=N (cm), namely complete one-shot measurement;
G. take out upper double dish 2, the erasing glycerine be attached to bottom it floats supporting agent 5, the root system in the lower double dish 1 of process, after rinsing lower double dish 1, can start second time and measure with water.
Embodiment two: utilize the present invention to carry out measuring the length of AMF mycelia in soil.
A. set the higher glass culture dish of two penetrabilitys, the bottom thickness wherein going up double dish is 0.1cm, and the bottom thickness of lower double dish 1 is 0.15cm, the interior diameter interior diameter of lower double dish is 8.8cm, overall diameter is 9.2cm, and the interior diameter of upper double dish is 8.2cm, and overall diameter is 8.6cm;
B. carry out suction filtration obtain AMF mycelia in soil on the filter membrane of 8cm diameter by drawing Soil Slurry, and with staining reagent, designing lattice length is 7/11cm, and utilizes printer to be printed on viscose paper, makes grid viscose paper 3;
C. in upper double dish 2, drip grape-kernel oil adsorbent 4 and evenly launch, then with scissors, printed grid viscose paper 3 is cut into the circle of diameter 8.1cm, then be laid in double dish 2, grid viscose paper 3 and upper double dish 2 rely on grape-kernel oil adsorbent 4 closely to stick together and bubble is not stayed in centre;
D. in lower double dish, 1 dropping several glycerine float supporting agent 5, facing up one of filter membrane band dyeing mycelia is put in lower double dish 1, and under Stereo microscope amplifies 40 times, observe confirmation can differentiate single mycelia, on filter membrane, then drip several glycerine float supporting agent 5;
E. will be put in lower double dish 1 with the upper double dish 2 printing grid viscose paper 3, filter membrane and lower double dish 1 are tightly attached together with upper double dish 2 by the surface tension that glycerine floats supporting agent 5, and mycelia can not float and filter membrane and do not stay bubble between upper double dish 2 and lower double dish 1;
F. lower double dish 1 is put on the objective table of Stereo microscope with the combination unit of upper double dish 2, add up under amplification 60 times mycelia and grid viscose paper 3 on filter membrane anyhow graticule line intersect sum N, substitute into formulae discovery survey the length R=11/14 × N × 7/11=N/2 (cm) of mycelia, namely complete one-shot measurement;
G. take out upper double dish 2, the erasing glycerine be attached to bottom it floats supporting agent 5, the filter membrane in the lower double dish 1 of process, after rinsing lower double dish 1, can start second time and measure with water.
Claims (1)
1. a very thin thread measurement of length method, is characterized in that:
A. the bottom thickness going up double dish (2) is 0.1cm, and the bottom thickness of lower double dish (1) is 0.15cm, and the interior diameter interior diameter of lower double dish (1) is 8.8cm, and overall diameter is 9.2cm, and the interior diameter of upper double dish (2) is 8.2cm, and overall diameter is 8.6cm;
B. carry out suction filtration obtain AMF mycelia in soil on the filter membrane of 8cm diameter by drawing Soil Slurry, and with staining reagent, designing lattice length is 7/11cm, and utilizes printer to be printed on viscose paper, makes grid viscose paper (3);
C. in upper double dish (2), drip grape-kernel oil adsorbent (4) and evenly launch, then with scissors, printed grid viscose paper (3) is cut into the circle of diameter 8.1cm, then be laid in double dish (2), grid viscose paper (3) and upper double dish (2) rely on grape-kernel oil adsorbent (4) closely to stick together and bubble is not stayed in centre;
D. in lower double dish, (1) drips several glycerine and floats supporting agent (5), facing up one of filter membrane band dyeing mycelia is put in lower double dish (1), and under Stereo microscope amplifies 40 times, observe confirmation can differentiate single mycelia, on filter membrane, then drip several glycerine float supporting agent (5);
E. will be put in lower double dish (1) with the upper double dish (2) printing grid viscose paper (3), filter membrane and lower double dish (1) are tightly attached together with upper double dish (2) by the surface tension that glycerine floats supporting agent (5), and mycelia can not float and filter membrane and do not stay bubble between upper double dish (2) and lower double dish (1);
F. lower double dish (1) and the combination unit of upper double dish (2) are put on the objective table of Stereo microscope, add up under amplification 60 times mycelia and grid viscose paper (3) on filter membrane anyhow graticule line intersect sum N, substitute into formulae discovery survey the length R=11/14 × N × 7/11=N/2 (cm) of mycelia.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310506144.XA CN103499299B (en) | 2013-10-24 | 2013-10-24 | A kind of very thin thread measurement of length method |
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| CN201310506144.XA CN103499299B (en) | 2013-10-24 | 2013-10-24 | A kind of very thin thread measurement of length method |
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| CN103499299A CN103499299A (en) | 2014-01-08 |
| CN103499299B true CN103499299B (en) | 2015-11-18 |
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| CN109539996B (en) * | 2018-12-12 | 2021-06-04 | 安徽省农业科学院水稻研究所 | Method for measuring length of rice stigma |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034994A (en) * | 1989-01-26 | 1989-08-23 | 东南大学 | Length measuring instrument for plant root system |
| CN102783417A (en) * | 2012-08-06 | 2012-11-21 | 云南农业大学 | Method for using glass tube for culturing straight root system plants for directly measuring root length in different stages |
| CN102783401A (en) * | 2012-08-06 | 2012-11-21 | 云南农业大学 | Method for culturing rice by using test tube water agar culture medium for measuring root length |
| CN102812892A (en) * | 2012-08-06 | 2012-12-12 | 云南农业大学 | Method for directly measuring root length at seedling stage by utilizing graduated test tubes to culture wheat |
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2013
- 2013-10-24 CN CN201310506144.XA patent/CN103499299B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1034994A (en) * | 1989-01-26 | 1989-08-23 | 东南大学 | Length measuring instrument for plant root system |
| CN102783417A (en) * | 2012-08-06 | 2012-11-21 | 云南农业大学 | Method for using glass tube for culturing straight root system plants for directly measuring root length in different stages |
| CN102783401A (en) * | 2012-08-06 | 2012-11-21 | 云南农业大学 | Method for culturing rice by using test tube water agar culture medium for measuring root length |
| CN102812892A (en) * | 2012-08-06 | 2012-12-12 | 云南农业大学 | Method for directly measuring root length at seedling stage by utilizing graduated test tubes to culture wheat |
Non-Patent Citations (1)
| Title |
|---|
| 快速测量植物根系长度的方法;刘顺康;《土壤》;19950430(第2期);第105-109页 * |
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