CN103487423B - Based on the method for quick whether plasticiser in the food of original position spraying process exceeds standard - Google Patents
Based on the method for quick whether plasticiser in the food of original position spraying process exceeds standard Download PDFInfo
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- CN103487423B CN103487423B CN201310478146.2A CN201310478146A CN103487423B CN 103487423 B CN103487423 B CN 103487423B CN 201310478146 A CN201310478146 A CN 201310478146A CN 103487423 B CN103487423 B CN 103487423B
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Abstract
The method for quick whether exceeded standard based on plasticiser in the food of original position spraying process belongs to field of detection of food safety, is specifically related to the method for quick of content of plasticizing agent in a kind of food; Whether first the method sets up critical plasticiser test stone, then check content of plasticizing agent to exceed standard; Due to the present invention according to content of plasticizing agent Critical Standard as detection reference, coordinate the strength information whether producing blue-fluorescence and fluorescence simultaneously, whether therefore whether the present invention not only can be used for differentiating containing plasticiser in food, and can exceed standard to content of plasticizing agent and judge; Owing to can select the various standard such as standard, the standard of adult, the standard of livestock of children for the selection of Critical Standard, therefore the present invention can form different content standards.
Description
Technical field
The method for quick whether exceeded standard based on plasticiser in the food of original position spraying process belongs to field of detection of food safety, is specifically related to the method for quick of content of plasticizing agent in a kind of food.
Background technology
In by the end of May, 2011, " plasticiser event " that Taiwan occurs, feeds through to China mainland area, and the country such as the U.S., Canada, Philippine, become international food security incident.Ministry of Health of China promptly issued bulletin June 1, and by plasticiser phthalate material, listing in may the non-edible material from soybeans of illegal interpolation and the food additives list of easy abuse in food.
Mist generating agent is a kind of regular food additives, and there is with it same effect, but the lower plasticiser of cost is illegal adjuvant, a lot of illegal retailer is in order to reduce costs, plasticiser is replaced mist generating agent, but its toxic and side effect can produce serious harm to health.Such as cause that male endocrine is disorderly, sperm quantity reduces, degradation problem under fecundity.Therefore be necessary in food whether containing plasticiser, whether content exceeds standard detects.
At present, in food, the detection method of " plasticiser " has GC method, HPLC method, GC-MS method etc., and the cost of these methods is all higher, and too professional, is not suitable for the common people to detect.The patent of invention rapid fluorescence detection method of Phthalates plasticiser " in the food ", to describe in a kind of food the method whether containing plasticiser.The method cost is low, and detection speed is fast, and goes for the common people to detect.But also there is following problem:
First, be not in food, have plasticiser to be exactly underproof, content of plasticizing agent should have threshold value, and the content of plasticizing agent lower than threshold value is fine, and therefore this detection means can not differentiate the food which contains plasticiser is qualified;
Secondly, this invention does not have content of plasticizing agent standard in concrete food, as children's standard, adult's standard etc.
Summary of the invention
In order to solve the problem, the invention discloses a kind of method for quick whether exceeded standard based on plasticiser in the food of original position spraying process, whether the method not only can be used for differentiating in food containing plasticiser, and whether can exceed standard to content of plasticizing agent and judge, different content standards can be formed simultaneously.
The object of the present invention is achieved like this:
Based on the method for quick whether plasticiser in the food of original position spraying process exceeds standard, comprise the following steps:
A, set up critical plasticiser test stone
Select solute: plasticiser;
Be equipped with solvent: take oxammonium hydrochloride 1-3g, sodium metavanadate 8-20mg, the acetic acid 20-40ml of Nickelous nitrate hexahydrate 0.16-0.3g, 50%-90%, is uniformly mixed;
Solute and solvent quality are mixed than according to content of plasticizing agent Critical Standard;
By mixed solution uniform application on the cover slip, at 90-120 DEG C, toast 1-10 minute with baking lamp, be the ultra violet lamp of 340-380nm with wavelength, obtain blue-fluorescence, using the gray values of this fluorescence as test stone;
Whether b, inspection content of plasticizing agent exceed standard
Be equipped with reactant liquor: take oxammonium hydrochloride 1-3g, sodium metavanadate 8-20mg, the acetic acid 20-40ml of Nickelous nitrate hexahydrate 0.16-0.3g, 50%-90%, is uniformly mixed;
Reactant liquor is sprayed to its surface, at 90-120 DEG C, toasting 1-10 minute with baking lamp, is the ultra violet lamp of 340-380nm with wavelength, if:
Do not obtain blue-fluorescence, then not containing plasticiser;
Obtain blue-fluorescence, but of light color than step a, then content of plasticizing agent does not exceed standard;
Obtain blue-fluorescence, but darker than the color of step a, then and content of plasticizing agent exceeds standard.
The above-mentioned method for quick whether exceeded standard based on plasticiser in the food of original position spraying process, described plasticiser is Phthalates plasticiser.
The above-mentioned method for quick whether exceeded standard based on plasticiser in the food of original position spraying process, the solution described in step a is identical with the reactant liquor described in step b.
The above-mentioned method for quick whether exceeded standard based on plasticiser in the food of original position spraying process, in the solution described in step a, each composition equal proportion adjusts.
The above-mentioned method for quick whether exceeded standard based on plasticiser in the food of original position spraying process, in the reactant liquor described in step b, each composition equal proportion adjusts.
The above-mentioned method for quick whether exceeded standard based on plasticiser in the food of original position spraying process, described step a performed once, and each testing process is:
Reactant liquor is sprayed to its surface, at 90-120 DEG C, toasting 1-10 minute with baking lamp, is the ultra violet lamp of 340-380nm with wavelength, if:
Do not obtain blue-fluorescence, then not containing plasticiser;
Obtain blue-fluorescence, but of light color than step a, then content of plasticizing agent does not exceed standard;
Obtain blue-fluorescence, but darker than the color of step a, then and content of plasticizing agent exceeds standard.
The above-mentioned method for quick whether exceeded standard based on plasticiser in the food of original position spraying process, described in step a using the gray values of this fluorescence as test stone, adopt make colorimetric card method formed standard.
The above-mentioned method for quick whether exceeded standard based on plasticiser in the food of original position spraying process, described in step a using the gray values of this fluorescence as test stone, adopt machine vision method formed standard.
Due to the present invention according to content of plasticizing agent Critical Standard as detection reference, coordinate the strength information whether producing blue-fluorescence and fluorescence simultaneously, whether therefore whether the present invention not only can be used for differentiating containing plasticiser in food, and can exceed standard to content of plasticizing agent and judge; Owing to can select the various standard such as standard, the standard of adult, the standard of livestock of children for the selection of Critical Standard, therefore the present invention can form different content standards.
Accompanying drawing explanation
Fig. 1 is the process flow diagram of the inventive method.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the invention is described in further detail.
Specific embodiment one
Based on the method for quick whether plasticiser in the food of original position spraying process exceeds standard, process flow diagram as shown in Figure 1.The method comprises the following steps:
A, set up critical plasticiser test stone
Select solute: plasticiser;
Be equipped with solvent: take oxammonium hydrochloride 1g, sodium metavanadate 8mg, Nickelous nitrate hexahydrate 0.16g, the acetic acid 20ml of 50%, is uniformly mixed;
Solute and solvent quality are mixed than according to content of plasticizing agent Critical Standard;
By mixed solution uniform application on the cover slip, toast 10 minutes at 100 DEG C with baking lamp, be the ultra violet lamp of 340nm with wavelength, obtain blue-fluorescence, using the gray values of this fluorescence as test stone; Be specially and make the blue colorimetric card identical with fluorescence color, as standard.
The mode of this making colorimetric card is convenient to comparison, and colorimetric card is convenient for carrying, and testing environment selectivity is larger.
Whether b, inspection content of plasticizing agent exceed standard
Be equipped with reactant liquor: take oxammonium hydrochloride 1g, sodium metavanadate 8mg, Nickelous nitrate hexahydrate 0.16g, the acetic acid 20ml of 50%, is uniformly mixed;
By reactant liquor uniform application on the cover slip, toasting 10 minutes at 100 DEG C with baking lamp, is the ultra violet lamp of 340nm with wavelength, if:
Do not obtain blue-fluorescence, then not containing plasticiser;
Obtain blue-fluorescence, but of light color than step a, then content of plasticizing agent does not exceed standard;
Obtain blue-fluorescence, but darker than the color of step a, then and content of plasticizing agent exceeds standard.
Specific embodiment two
The present embodiment is the gray values of this fluorescence as test stone from the different of specific embodiment one; Be specially the method adopting machine vision, obtain gray scale or the RGB numerical value of fluorescence, as standard.
The mode of this machine vision is convenient to automatically judge fast, and the metrical error effectively avoiding subjective judgement to bring, and improves detection confidence.
Specific embodiment three
The present embodiment is from the different of specific embodiment one, and after step a performs, critical plasticiser test stone is set up, and therefore, check without the need to repeated execution of steps a, therefore, the step of the method is as follows at every turn:
By reactant liquor uniform application identical for the solvent used with step a on the cover slip, toasting 10 minutes at 100 DEG C with baking lamp, is the ultra violet lamp of 340nm with wavelength, if:
Do not obtain blue-fluorescence, then not containing plasticiser;
Obtain blue-fluorescence, but of light color than step a, then content of plasticizing agent does not exceed standard;
Obtain blue-fluorescence, but darker than the color of step a, then and content of plasticizing agent exceeds standard.
Specific embodiment four
The present embodiment is from the different of specific embodiment two, and after step a performs, critical plasticiser test stone is set up, and therefore, check without the need to repeated execution of steps a, therefore, the step of the method is as follows at every turn:
By reactant liquor uniform application identical for the solvent used with step a on the cover slip, toasting 10 minutes at 100 DEG C with baking lamp, is the ultra violet lamp of 340nm with wavelength, if:
Do not obtain blue-fluorescence, then not containing plasticiser;
Obtain blue-fluorescence, but of light color than step a, then content of plasticizing agent does not exceed standard;
Obtain blue-fluorescence, but darker than the color of step a, then and content of plasticizing agent exceeds standard.
The method for quick whether exceeded standard based on plasticiser in the food of original position spraying process of above embodiment, described plasticiser is Phthalates plasticiser.
The method for quick whether exceeded standard based on plasticiser in the food of original position spraying process of above embodiment, described content of plasticizing agent Critical Standard is with reference to U.S. food safety standard or European Food safety standard.
The present invention is not limited to above-mentioned preferred forms, and anyone should learn the structure change or method improvement made under enlightenment of the present invention, and every have identical or close technical scheme with the present invention, all falls within protection scope of the present invention.
Claims (3)
1., based on the method for quick whether plasticiser in the food of original position spraying process exceeds standard, it is characterized in that comprising the following steps:
A, set up critical plasticiser test stone
Select solute: plasticiser;
Be equipped with solvent: take oxammonium hydrochloride 1-3g, sodium metavanadate 8-20mg, the acetic acid 20-40ml of Nickelous nitrate hexahydrate 0.16-0.3g, 50%-90%, is uniformly mixed;
Solute and solvent quality are mixed than according to content of plasticizing agent Critical Standard;
By mixed solution uniform application on the cover slip, at 90-120 DEG C, toast 1-10 minute with baking lamp, be the ultra violet lamp of 340-380nm with wavelength, obtain blue-fluorescence, adopt the method for machine vision to obtain gray scale or the RGB numerical value of fluorescence, as test stone;
Whether b, inspection content of plasticizing agent exceed standard
Be equipped with the reactant liquor identical with the solvent described in step a: take oxammonium hydrochloride 1-3g, sodium metavanadate 8-20mg, the acetic acid 20-40ml of Nickelous nitrate hexahydrate 0.16-0.3g, 50%-90%, is uniformly mixed;
Reactant liquor is sprayed to food surface to be measured, and at 90-120 DEG C, toasting 1-10 minute with baking lamp, is the ultra violet lamp of 340-380nm with wavelength, if:
Do not obtain blue-fluorescence, then not containing plasticiser;
Obtain blue-fluorescence, but of light color than step a, then content of plasticizing agent does not exceed standard;
Obtain blue-fluorescence, but darker than the color of step a, then and content of plasticizing agent exceeds standard;
Described plasticiser is phthalate plasticiser.
2. the method for quick whether exceeded standard based on plasticiser in the food of original position spraying process according to claim 1, is characterized in that in the solvent described in step a and the reactant liquor described in step b, each composition equal proportion adjusts.
3. the method for quick whether exceeded standard based on plasticiser in the food of original position spraying process according to claim 1, it is characterized in that described step a performed once, each testing process is:
Reactant liquor is sprayed to food surface to be measured, and at 90-120 DEG C, toasting 1-10 minute with baking lamp, is the ultra violet lamp of 340-380nm with wavelength, if:
Do not obtain blue-fluorescence, then not containing plasticiser;
Obtain blue-fluorescence, but of light color than step a, then content of plasticizing agent does not exceed standard;
Obtain blue-fluorescence, but darker than the color of step a, then and content of plasticizing agent exceeds standard.
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| CN201310478146.2A CN103487423B (en) | 2013-10-14 | 2013-10-14 | Based on the method for quick whether plasticiser in the food of original position spraying process exceeds standard |
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