CN103484540A - Method for identifying expression level of TIGAR gene and special primers thereof - Google Patents
Method for identifying expression level of TIGAR gene and special primers thereof Download PDFInfo
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Abstract
The invention discloses a method for identifying expression level of TIGAR gene and special primers thereof. The primer pair disclosed by the invention is composed of a single chain DNA (deoxyribonucleic acid) molecule disclosed as Sequence 1 in the sequence table and a single chain DNA molecule disclosed as Sequence 2 in the sequence table. The specific primer pair can be used for identification of the TIGAR gene, identification of the expression level of the TIGAR gene in pork food, and assisted identification of pork quality. An SYBR GreenI fluorescent quantitative PCR (polymerase chain reaction) method is utilized to research the expression level of the TIGAR gene by using the specific primer pair and establish a TIGAR gene expression standard curve, wherein the linear relationship is good. The primer pair and method disclosed by the invention have the advantages of high sensitivity, high specificity, high accuracy, high reliability and the like, and can be used for detecting the expression level of the porcine TIGAR gene.
Description
Technical field
The present invention relates to a kind of method and primer special thereof of expression amount of the TIGAR of evaluation gene.
Background technology
PSE meat is a kind of pale, soft, meat of poor quality of having juice to ooze out, and the annual bad change of meat caused by it has caused huge financial loss to the livestock and poultry industry.The formation of PSE meat depends primarily on the speed of butchering rear anaerobic glycolysis, and the speed of anaerobic glycolysis is faster, more easily produces PSE meat.
Recently, Britain Beatson ICR and Spain researchist find a kind of TIGAR(TP53-induced glycolysis and apoptosis regulator, the glycolysis-that TP53 induces and Apoptosis of being called in antitumor research process) the p53 induced gene.TIGAR albumen can be regulated aerobic repiration and glycolysis-balance.TIGAR albumen has fructose-2,6-diphosphatase (FBPase-2) activity, TIGAR expresses and makes fructose-2,6-bisphosphate dephosphorylation becomes fructose-6-phosphate, reduced its content in cell, and fructose-2, the 6-bisphosphate is the powerful incitant of glycolysis-key enzyme phosphofructokinase (PFK-1), fructose-2, the reduction of 6-bisphosphate level can suppress the activity of phosphofructokinase, and glycolysis reaction speed is descended, and causes the variation of a series of enzymes of glycolysis-and product, the pH lowering speed slows down, and suppresses the generation of PSE meat.
Summary of the invention
The method and the primer special thereof that the purpose of this invention is to provide a kind of expression amount of the TIGAR of evaluation gene.
Primer special provided by the invention is a pair of special primer (claims again special primer to), the single strand dna shown in the sequence 2 of the single strand dna shown in the sequence 1 of sequence table and sequence table, consists of.
The present invention also protects described special primer to the application in identifying the TIGAR gene.
The present invention also protects described special primer to the application in the expression amount of TIGAR gene in identifying live fresh pork.
The present invention also protects described special primer to the application in the assistant identification meat quality.
The present invention also protects described special primer to the application in preparing test kit; The function of described test kit is following (a) or (b) or (c): (a) identify the TIGAR gene; (b) identify the expression amount of TIGAR gene in live fresh pork; (c) assistant identification meat quality.
The present invention also protects a kind of test kit, comprises described special primer pair; The function of described test kit is following (a) or (b) or (c): (a) identify the TIGAR gene; (b) identify the expression amount of TIGAR gene in live fresh pork; (c) assistant identification meat quality.Described test kit also can comprise the primer pair for the identification of reference gene, and described reference gene specifically can be β-actin gene.The described primer pair for the identification of reference gene specifically can be the primer pair that the single strand dna shown in the sequence 4 of the single strand dna shown in the sequence 3 of sequence table and sequence table forms.Also can comprise the standard substance plasmid with the right target sequence of described special primer in described test kit.Described standard substance plasmid specifically can be the double chain DNA molecule shown in the sequence of sequence table 5 is inserted to the recombinant plasmid that skeleton carrier obtains.Described skeleton carrier specifically can be the pMD-18T carrier.Described test kit also can comprise the carrier that records the typical curve equation; Described typical curve equation can be: y=-3.0356lgx+11.402, R
2=0.9995, y is cycle threshold, the copy number that x is TIGAR gene fragment in system.
The present invention also protects a kind of method of identifying the expression amount of TIGAR gene in pork, comprises the steps:
(1) total RNA the reverse transcription of extracting pork to be measured are cDNA;
(2) take the cDNA that step (1) obtains is template, adopts described special primer to carrying out real-time fluorescence PCR;
(3) obtain the expression amount of TIGAR gene according to the result of described real-time fluorescence PCR.
The reaction conditions of described real-time fluorescence PCR specifically can be: 95 ℃ of 30s; 95 ℃ of 5s, 64 ℃ of 47s, 40 circulations.
Above arbitrary described TIGAR gene specifically can be (I), (II) or (III) as follows: the DNA molecular shown in sequence 6 in (I) sequence table; The DNA molecular of the albumen that the DNA sequence dna hybridization that (II) limits to (I) under stringent condition and coding are relevant with PSE meat; The DNA sequence dna that (III) and (I) limit has the DNA molecular of 90% above homology and the coding albumen relevant with PSE meat.Above-mentioned stringent condition can be at 6 * SSC, in the solution of 0.5%SDS, under 65oC, hybridizes, and then uses 2 * SSC, 0.1%SDS and 1 * SSC, 0.1%SDS respectively to wash film once.
In view of the TIGAR gene pairs reduces the glycolysis-level, suppress the certain effect that has of PSE meat, and very few to the two expression conditions research both at home and abroad of killing rear different varieties, different time at present.The invention provides special primer pair, adopt SYBR Green I fluorescence quantifying PCR method to be studied the TIGAR expression conditions, set up TIGAR genetic expression typical curve and linear relationship good.The invention provides to primer pair and method there is highly sensitive, high specificity, wait advantage accurately and reliably, can be used for detecting pig TIGAR expression conditions.
The accompanying drawing explanation
Fig. 1 is the amplification curve in embodiment 2.
Fig. 2 is the solubility curve in embodiment 2.
Fig. 3 adopts 1.5% agarose gel electrophoresis to detect the electrophorogram of the integrity of RNA in embodiment 3.
Fig. 4 is the electrophorogram that passes through the quality of detection β-actin gene identification cDNA in embodiment 3.
Fig. 5 detects the amplification curve of TIGAR gene in embodiment 3.
Fig. 6 detects the solubility curve of TIGAR gene in embodiment 3.
Fig. 7 detects the typical curve of TIGAR gene in embodiment 3.
Fig. 8 detects the amplification curve of reference gene in embodiment 3.
Fig. 9 detects the solubility curve of reference gene in embodiment 3.
Figure 10 detects the typical curve of reference gene in embodiment 3.
The result that Figure 11 is embodiment 4.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, be ordinary method.Test materials used in following embodiment, if no special instructions, be and purchase available from routine biochemistry reagent shop.Quantitative test in following examples, all arrange repeated experiments three times, results averaged.
PMD-18T carrier: Dalian precious biotechnology company limited; Takara Code:6011.E.coli JM109 competent cell: Dalian precious biotechnology company limited; Takara Code:D9052S.100bp DNA Ladder Marker: Dalian precious biotechnology company limited; Takara Code:3422A.SYBRPremix Ex TaqII: Dalian precious biotechnology company limited; Takara Code:RR820Q.2 * Power Taq PCR MasterMix: hundred Tyke Bioisystech Co., Ltd, article No. PR1701.Universal DNA purifying reclaims test kit: day root biochemical technology company limited, article No. DP214-02.The high purity plasmid is little carries middle amount test kit: day root biochemical technology company limited, article No. DP107-02.
Design a pair of primer for the identification of the TIGAR gene following (target sequence is 169bp):
F1(sequence 1): 5 '-CAggTgAAAATgCgTggAAA-3 ';
R1(sequence 2): 5 '-CTgAATTAAACgTggAggCACA-3 '.
Design a pair of primer pair for the identification of β-actin gene following (target sequence is 216bp):
F2(sequence 3): 5 '-TgCgggACATCAAggAgAAg-3 ';
R2(sequence 4): 5 '-AgTTgAAggTggTCTCgTgg-3 '.
The sensitivity of embodiment 2, primer pair and specificity
1, the double chain DNA molecule shown in the sequence 5 of composition sequence table, be inserted into the pMD-18T carrier, obtains recombinant plasmid.
2, by plasmid concentration, be 3.4 * 10
9the recombinant plasmid solution of copy number/μ l carries out gradient dilution with ultrapure water, then using each diluent as template, carries out respectively real-time fluorescence PCR.
Real-time fluorescence PCR system (20 μ l): 10 μ l SYBRPremix Ex TaqII, 0.8 μ l F1,0.8 μ l R1,0.4 μ l ROX Reference Dye, 2 μ l templates, 6 μ l dH
2o.
Real-time fluorescence PCR adopts AB7500Real Time quantitative real time PCR Instrument to carry out, and reaction conditions is: 95 ℃ of 30s; 95 ℃ of 5s, 64 ℃ of 47s, 40 circulations; Finally add instrument to carry the solubility curve program.
Every kind of diluent carries out repeated experiments three times.
Amplification curve is shown in Fig. 1.In Fig. 1, amplification curve from left to right is followed successively by and adopts plasmid concentration is 3.4 * 10
8, 3.4 * 10
7, 3.4 * 10
6, 3.4 * 10
5, 3.4 * 10
4, 3.4 * 10
3, 3.4 * 10
2, 3.4 * 10
1the amplification curve that the diluent of copy number/μ l obtains as template.In the real-time fluorescence PCR system, the copy number of TIGAR gene fragment is 10 when above, all can be observed amplification curve, the primer pair that adopts F1 and R1 to form is identified goal gene by real-time fluorescence PCR, the goal gene that sensitivity is 10 copies/each reaction system.
Contain 10 in the PCR system
1-10
8during the TIGAR gene fragment of order of magnitude copy, the logarithm of Ct value and copy number is linear, according to each diluent, carries out the curve of production standard as a result of real-time fluorescence PCR and obtain the typical curve equation as follows: y=-3.0356lgx+11.402, R
2=0.9995, y is cycle threshold, the copy number that x is TIGAR gene fragment in system.
Solubility curve is shown in Fig. 2, only has as seen simple spike, illustrates that real-time fluorescence PCR has obtained single product, high specificity.F1 and R1 are compared on NCBI known, this primer pair TIGAR gene order that can only increase in the complete genome sequence of pig, therefore pollute under prerequisite strict control, while only increasing the pig cDNA storehouse, can not produce other non-specific amplification.
The application of embodiment 3, primer pair
1, get the semimembranosus that the Large White after 30min is butchered at two, extract total RNA.Adopt concentration and the purity of spectrophotometric determination RNA, the OD of total RNA
260/280between 1.7-2.0.Adopt 1.5% agarose gel electrophoresis to detect the integrity of RNA, see Fig. 3 (the 1 and 2 corresponding two of difference Large White), 28S and 18S band are high-visible, without obvious degradation.
Total RNA reverse transcription of 2, step 1 being extracted is cDNA.By detecting the quality of β-actin gene (reference gene) identification of cdna, 1.5% agarose gel electrophoresis figure is shown in Fig. 4 (the 1 and 2 corresponding two of difference Large White), shows the amplified band of reference gene, and concentration is more unified, and quality is better.
The PCR system: 12.5 μ l2 * Power Taq PCR MasterMix, cDNA1.5 μ l, F21.5 μ l, R21.5 μ l, add DEPC and process water to be supplemented to volume be 25 μ l.
PCR reaction conditions: 95 ℃ of denaturation 5min; 95 ℃ of sex change 30s, 55.5 ℃ of annealing 30s, 72 ℃ of extension 30s, 28 circulations; 72 ℃ are extended 10min.
3, cDNA step 2 obtained is diluted to 5 times of volumes, 10 times of volumes, 100 times of volumes, 1000 times of volumes with ultrapure water, obtains each diluent (5 times of diluents of called after, 10 times of diluents, 100 times of diluents or 1000 times of diluents successively).
4, each diluent step 3 obtained, as template, carries out respectively real-time fluorescence PCR, detects the TIGAR gene.
Real-time fluorescence PCR system (20 μ l): 10 μ l SYBRPremix Ex TaqII, 0.8 μ l F1,0.8 μ l R1,0.4 μ l ROX Reference Dye, 2 μ l templates, 6 μ l dH
2o.
Real-time fluorescence PCR adopts AB7500Real Time quantitative real time PCR Instrument to carry out, and reaction conditions is: 95 ℃ of 30s; 95 ℃ of 5s, 64 ℃ of 47s, 40 circulations; Finally add instrument to carry the solubility curve program.
Every kind of diluent carries out repeated experiments three times.
Amplification curve is shown in Fig. 5 (amplification curve from left to right is followed successively by 5 times of diluents, 10 times of diluents, 100 times of diluents or 1000 times of amplification curves that diluent obtains as template).Solubility curve is shown in Fig. 6.Typical curve is shown in Fig. 7.Under 5,10,100 or 1000 extension rates, linear relationship is good, R
2=0.999385, the typical curve equation is: Y=-3.469220lgX+34.441328, and wherein Y is cycle threshold, the copy number that X is TIGAR gene fragment in system, can calculate amplification efficiency is 94.2%.
5, each diluent step 3 obtained, as template, carries out respectively real-time fluorescence PCR, detects β-actin gene (reference gene).
Real-time fluorescence PCR system (20 μ l): 10 μ l SYBRPremix Ex TaqII, 0.8 μ l F2,0.8 μ l R2,0.4 μ l ROX Reference Dye, 2 μ l templates, 6 μ l dH
2o.
Real-time fluorescence PCR adopts AB7500Real Time quantitative real time PCR Instrument to carry out, and reaction conditions is: 95 ℃ of 30s; 95 ℃ of 5s, 64 ℃ of 47s, 40 circulations; Finally add instrument to carry the solubility curve program.
Every kind of diluent carries out repeated experiments three times.
Amplification curve is shown in Fig. 8 (amplification curve from left to right is followed successively by 5 times of diluents, 10 times of diluents, 100 times of diluents or 1000 times of amplification curves that diluent obtains as template).Solubility curve is shown in Fig. 9.Typical curve is shown in Figure 10.The typical curve equation is: Y=-3.277885lgX+23.205946, wherein Y is cycle threshold, the copy number that X is β in system-actin gene fragment, R
2=0.998073, linear relationship is better, and can calculate amplification efficiency is 101.8%.
Known according to the result of step 4 and step 5: each gene amplification curve reaction is good; Solubility curve only has a peak, illustrates that atopic is good; Each typical curve linear relationship is good, and target gene and reference gene amplification efficiency are basically identical.
The application of embodiment 4, primer pair
Adopt the method identical with embodiment 3, relative expression quantity to the TIGAR gene in the pig semimembranosus of butchering rear 30min, 2h, 4h, 8h is measured, and observes and kills rear different varieties (landrace and Northeast China grey bristle pig), different time pig TIGAR gene variation tendency.Landrace physique a little less than, resistance is poor, stress sensitive is stronger, therefore in order to be studied as more easily producing PSE meat kind.The Northeast China grey bristle pig is to take local people pig as the maternal peculiar kind of China formed with the hybridization of grammeter Lip river husband pig, its growth is very fast, feed conversion rate is high, strong adaptability, carcass lean meat percentage is lower, be difficult for generation stress, pretend for be difficult for to produce PSE meat kind with long carry out in vain TIGAR mRNA kill after the comparative studies of different time expression amount.
Carry out altogether 4 parallel tests, after the landrace of usining kills the TIGAR gene expression amount of 30min as reference, other each kind, each kill after the time with respect to the relative expression quantity of reference in Table 1.Through SAS9.1.3, analyze, conclusion is as follows: each kind kill after 30min with kill after 2,4, there were significant differences for 8h, the 30min expression amount is significantly higher than 2,4,8h(P<0.05); Between Northeast China grey bristle pig and landrace, there were significant differences for the TIGAR gene expression amount, and after Northeast China grey bristle pig government official, the expression amount of each time point all is significantly higher than landrace (P<0.05).
The relative expression quantity of different time TIGAR gene after the pig of table 1 different varieties kills
| ? | Landrace | The Northeast China grey bristle |
| 30min | ||
| 1 Dd | 3.745954±0.67494 Bb | |
| 2h | 0.957714±0.150667 Ee | 0.647671±0.077663 Ee |
| 4h | 0.398873±0.096261 Ee | 0.593094±0.083953 Ee |
| 8h | 0.453445±0.050641 Ee | 0.193535±0.00101 Ee |
Annotate: the different difference extremely significantly (P<0.01) that means of capitalization, the different significant differences (P<0.05) that mean of lowercase.
TIGAR mRNA is mapped and carries out multiple comparisons, see Figure 11.In Figure 11: every two adjacent pillars are one group, and the pillar on the left side is landrace in every group, and the pillar on the right is the Northeast China grey bristle pig; The different difference extremely significantly (P<0.01) that means of capitalization, the different significant differences (P<0.05) that mean of lowercase.Through SAS9.1.3, analyze, conclusion is as follows: Northeast China grey bristle pig TIGAR gene expression amount is significantly higher than landrace (P<0.05); After landrace kills, there were significant differences with killing rear 2h for 30min, and there were significant differences for 2h and 4h, 8h (P<0.05); After the Northeast China grey bristle pig kills, 30min and landrace 0h have utmost point significant difference (P<0.01), and after the rear 30min of landrace government official and Northeast China grey bristle pig kill, 8h has utmost point significant difference (P<0.01), and all the other kill rear time there was no significant difference; After the Northeast China grey bristle pig kills 30min with landrace, kill after 30min, 2h there were significant differences (P<0.05), after landrace kills 30min, 2h with landrace, kill after 4, there were significant differences (P<0.05) for 8h and the rear 8h of Northeast China grey bristle pig government official.
Claims (10)
1. primer pair, be comprised of the single strand dna shown in the sequence 2 of the single strand dna shown in the sequence 1 of sequence table and sequence table.
2. the application of the described primer pair of claim 1 in identifying the TIGAR gene.
3. the application in the expression amount of the described primer pair of claim 1 TIGAR gene in identifying live fresh pork.
4. the application of the described primer pair of claim 1 in the assistant identification meat quality.
5. the application of the described primer pair of claim 1 in preparing test kit; The function of described test kit is following (a) or (b) or (c): (a) identify the TIGAR gene; (b) identify the expression amount of TIGAR gene in live fresh pork; (c) assistant identification meat quality.
6. a test kit, comprise the described primer pair of claim 1; The function of described test kit is following (a) or (b) or (c): (a) identify the TIGAR gene; (b) identify the expression amount of TIGAR gene in live fresh pork; (c) assistant identification meat quality.
7. test kit as claimed in claim 6, it is characterized in that: described test kit also comprises the standard substance plasmid of the target sequence with described primer pair.
8. test kit as described as claim 6 or 7, it is characterized in that: described test kit also comprises the primer pair for the identification of reference gene.
9. a method of identifying the expression amount of TIGAR gene in pork, comprise the steps:
(1) total RNA the reverse transcription of extracting pork to be measured are cDNA;
(2) take the cDNA that step (1) obtains is template, adopts the described primer pair of claim 1 to carry out real-time fluorescence PCR;
(3) obtain the expression amount of TIGAR gene in pork to be measured according to the result of described real-time fluorescence PCR.
10. method as claimed in claim 9, it is characterized in that: the reaction conditions of described real-time fluorescence PCR is: 95 ℃ of 30s; 95 ℃ of 5s, 64 ℃ of 47s, 40 circulations.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111420070A (en) * | 2020-04-24 | 2020-07-17 | 苏州大学 | Application of TIGAR gene or protein in preparation of radioactive gastrointestinal syndrome treatment drug |
| WO2021212653A1 (en) * | 2020-04-24 | 2021-10-28 | 苏州大学 | Method for screening treatment target for acute radiation gastrointestinal syndrome, and application of tigar target in preparation of drug for treating radiation gastrointestinal syndrome |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013096455A1 (en) * | 2011-12-20 | 2013-06-27 | Dana-Farber Cancer Institute, Inc. | Methods for diagnosing and treating oncogenic kras-associated cancer |
-
2013
- 2013-09-05 CN CN201310400073.5A patent/CN103484540A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013096455A1 (en) * | 2011-12-20 | 2013-06-27 | Dana-Farber Cancer Institute, Inc. | Methods for diagnosing and treating oncogenic kras-associated cancer |
Non-Patent Citations (4)
| Title |
|---|
| BOWKER ET AL: "Muscle metabolism and PSE pork", 《JOURNAL OF ANIMAL SCIENCE》 * |
| CHRISTINA WANKA ET AL: "Tp53-induced Glycolysis and Apoptosis Regulator (TIGAR) Protects Glioma Cells from Starvation-induced Cell Death by Up-regulating Respiration and Improving Cellular Redox Homeostasis", 《THE JOURNAL OF BIOLOGICAL CHEMISTRY》 * |
| 国家基金委: "2010年度国家基金委食品科学学科资助项目", 《2010年度国家基金委食品科学学科资助项目》 * |
| 无: "XM_001926543", 《NCBI REFSEQ NUCLEIC》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111420070A (en) * | 2020-04-24 | 2020-07-17 | 苏州大学 | Application of TIGAR gene or protein in preparation of radioactive gastrointestinal syndrome treatment drug |
| CN111420070B (en) * | 2020-04-24 | 2020-10-30 | 苏州大学 | Application of TIGAR gene or protein in preparation of radioactive gastrointestinal syndrome treatment drug |
| WO2021212653A1 (en) * | 2020-04-24 | 2021-10-28 | 苏州大学 | Method for screening treatment target for acute radiation gastrointestinal syndrome, and application of tigar target in preparation of drug for treating radiation gastrointestinal syndrome |
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