CN103483299B - A kind of extracting method of rographolide and application - Google Patents
A kind of extracting method of rographolide and application Download PDFInfo
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- CN103483299B CN103483299B CN201310466097.0A CN201310466097A CN103483299B CN 103483299 B CN103483299 B CN 103483299B CN 201310466097 A CN201310466097 A CN 201310466097A CN 103483299 B CN103483299 B CN 103483299B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
- C07D307/34—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
- C07D307/56—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D307/60—Two oxygen atoms, e.g. succinic anhydride
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/365—Lactones
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention provides a kind of extracting method of rographolide, supercritical extraction and microwave extracting is adopted to be prepared from, content is improved a lot, present invention also offers and provide rographolide to suppress the application in mouse EAT cell ECA cell proliferation and the application in preparation increase bone density medicine in preparation.
Description
Technical field
The present invention relates to technical field of extraction of Chinese traditional medicine, be specifically related to a kind of extracting method and application of rographolide.
Background technology
Rographolide, molecular formula C
20h
30o
5, natural phant Herba Andrographis mainly contain effective constituent, there is thermal detoxification of dispelling, effect of anti-inflammatory analgetic, have special efficacy to bacillary with viral upper respiratory tract infection and dysentery, be described as natural antibiotics medicine.This product is diterpenes diterpenoids lactones compound, is all insoluble in water, usually only can oral administration.Folium Andrographis 95% alcohol immersion, gained alcohol immersion gets liquid activated carbon decolorizing, concentrated solution after destainer Distillation recovery ethanol leaves standstill and obtains coarse-grain product, coarse-grain adds 15 times amount 95% ethanol heating for dissolving, activated carbon decolorizing, filtered while hot, leaves standstill recrystallization, obtain faint yellow recrystallization product, then refine obtain rographolide finished product through distilled water, chloroform, methanol wash.To crude drug meter, total recovery 1-1.5%.
In prior art, not yet there is rographolide in extraction preparation, adopt report that is overcritical and microwave technology, and adopt the method for alcohol extracting, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, had a strong impact on this product and applied clinically.
Summary of the invention
Goal of the invention: in order to solve the problem, the object of the present invention is to provide a kind of extracting method of rographolide.
Another object of the present invention is to provide rographolide to suppress the application in mouse EAT cell ECA cell proliferation and the application in preparation increase bone density medicine in preparation.
Technical scheme: the object of the invention is by following scheme realize:
An extracting method for rographolide, gets Herba Andrographis, joins CO
2in supercritical extraction device, ethanol is as entrainment agent, and the volume percent that entrainment agent accounts for total extraction solvent is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, add 70% ethanol, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W, extract 2 times, each 4-8 minute, the amount of 70% ethanol at every turn added is 2 times of medicinal material weight, obtain extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collects 5 times amount column volume elutriants, decompression recycling ethanol, concentrated and dry, obtain andrographolide extract.
The extracting method of above-mentioned rographolide, described CO
2the volume percent that supercritical extraction entrainment agent accounts for total extraction solvent is 5%.
The extracting method of above-mentioned rographolide, described CO
2the extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO
2flow 2ml/g crude drug min, extraction time 160min.
The extracting method of above-mentioned rographolide, described microwave extracting power 500W, each extraction 6 minutes.
Above-mentioned rographolide suppresses the application in mouse EAT cell ECA cell proliferation in preparation, and the extraction step of rographolide is: get Herba Andrographis, join CO
2in supercritical extraction device, ethanol is as entrainment agent, and the volume percent that entrainment agent accounts for total extraction solvent is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, add 70% ethanol, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W, extract 2 times, each 4-8 minute, the amount of 70% ethanol at every turn added is 2 times of medicinal material weight, obtain extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collects 5 times amount column volume elutriants, decompression recycling ethanol, concentrated and dry, obtain andrographolide extract.
Above-mentioned rographolide suppresses the application in mouse EAT cell ECA cell proliferation in preparation, CO described in the extracting method of rographolide
2the volume percent that supercritical extraction entrainment agent accounts for total extraction solvent is 5%, described CO
2the extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO
2flow 2ml/g crude drug min, extraction time 160min, described microwave extracting power 500W, each extraction 6 minutes.
Above-mentioned rographolide increases the application in bone density medicine in preparation, and the extraction step of rographolide is: get Herba Andrographis, join CO
2in supercritical extraction device, ethanol is as entrainment agent, and the volume percent that entrainment agent accounts for total extraction solvent is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, add 70% ethanol, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W, extract 2 times, each 4-8 minute, the amount of 70% ethanol at every turn added is 2 times of medicinal material weight, obtain extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collects 5 times amount column volume elutriants, decompression recycling ethanol, concentrated and dry, obtain andrographolide extract.
Above-mentioned rographolide increases the application in bone density medicine in preparation, CO described in the extracting method of rographolide
2the volume percent that supercritical extraction entrainment agent accounts for total extraction solvent is 5%, described CO
2the extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO
2flow 2ml/g crude drug min, extraction time 160min, described microwave extracting power 500W, each extraction 6 minutes.
In prior art, rographolide adopts the method for alcohol extracting, technique is coarse, backward, and impurity is many, causes patient's consumption excessive, be inconvenient to take, rographolide yield prepared by the present invention increases, and content is high, and purity reaches 95-96%, prior art does not find that it suppresses the application in mouse EAT cell ECA cell proliferation and the application in preparation increase bone density medicine in preparation, shows that this product is hopeful to make such medicine.
Embodiment
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following example, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Get Herba Andrographis 1000g, join CO
2in supercritical extraction device, ethanol is as entrainment agent, and the volume percent that entrainment agent accounts for total extraction solvent is 4%, extracting pressure 15MPa, temperature 30 DEG C, CO
2flow 1m1/g crude drug min, extraction time 150min, obtains supercritical extract, add 70% ethanol again, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 400W, extract 2 times, each 4 minutes, 70% amount of alcohol at every turn added is 2000g, obtain extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collects 5 times amount column volume elutriants, decompression recycling ethanol, concentrated and dry, obtaining andrographolide extract, is 95.3% through high effective liquid chromatography for measuring content.Embodiment 2
Get Herba Andrographis 1000g, join CO
2in supercritical extraction device, ethanol is as entrainment agent, and the volume percent that entrainment agent accounts for total extraction solvent is 6%, extracting pressure 30MPa, temperature 50 C, CO
2flow 3m1/g crude drug min, extraction time 180min, obtains supercritical extract, add 70% ethanol again, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 600W, extract 2 times, each 8 minutes, 70% amount of alcohol at every turn added is 2000g, obtain extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collects 5 times amount column volume elutriants, decompression recycling ethanol, concentrated and dry, obtaining andrographolide extract, is 95.8% through high effective liquid chromatography for measuring content.
Embodiment 3
Get Herba Andrographis 1000g, join CO
2in supercritical extraction device, ethanol is as entrainment agent, and the volume percent that entrainment agent accounts for total extraction solvent is 5%, extracting pressure 20MPa, temperature 40 DEG C, CO
2flow 2m1/g crude drug min, extraction time 160min, obtains supercritical extract, add 70% ethanol again, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 500W, extract 2 times, each 6 minutes, 70% amount of alcohol at every turn added is 2000g, obtain extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collects 5 times amount column volume elutriants, decompression recycling ethanol, concentrated and dry, obtaining andrographolide extract, is 95.9% through high effective liquid chromatography for measuring content.
In above-described embodiment, Herba Andrographis is the herb of acanthaceous plant Herba Andrographis AndrographisPaniculata (Burm.f) Ness.
Embodiment 4: rographolide suppresses the experimental study data of mouse EAT cell ECA cell proliferation
1 experiment material
1.1 experiment cell strains
Mouse EAT cell (ECA), Nanjing Zhengliang Pharmaceutical Technology Co., Ltd.'s laboratory cell storehouse, DMEM+10%FBS cellar culture.
1.2 Experimental agents
Drugs: rographolide of the present invention: prepare by embodiment 3 method.
Liquid liquid storage: take 100mg rographolide, is dissolved in 5ml dehydrated alcohol, 0.2 μm of frit, 500 μ ldoff pipe packing ,-20 DEG C of storages, and simultaneously 0.2 μm of frit dehydrated alcohol is in order to the use of control group.
1.3 experiment reagent
DMEM(GIBCO company Cat.No.12100-061Lot.No.758137); Foetal calf serum (Tian Hang bio tech ltd, Zhejiang Lot.No.100419); NaHCO3(Shanghai hundred million chemical reagent company limited Cat.No.11810-033Lot.No.1088387 of a specified duration); Trypsin(AMRESCO company lot number: 2010/04); EDTA(AMRESCO company lot number: 2009/10); PenicillinGSodiumSalt(AMRESCO company lot number: 2010242); StreptomycinSulfate(AMRESCO company lot number: 2010382); Dehydrated alcohol (Nanjing Chemistry Reagent Co., Ltd.'s lot number: 080310182); MTT (Biosharp lot number: 0793); PBS(laboratory autogamy); 1.4 experiment equipment
Lycra inverted microscope (German Leica model: DM1L); Visible-UV-light microwell plate detector (MD company of U.S. model: SPECTRAMAX190); CO2 incubator (FORMA model: 3111); Super clean bench (safe and sound Inc. of Su Jing group moulding number: SW-CJ-ZFD); Pure water instrument (Spring company of U.S. model: S/N020579); Accurate pipettor (French Gilson Inc model: P2); Electronic balance (German Sai Duolisi company limited model: BT323S); Full-automatic high-pressure Autoclave (Japanese SANYO company model: MLS-3020); Table electrothermal air dry oven (the accurate experimental installation company in Shanghai model: DHG9123A); Refrigerator (Siemens Company's model: KG18V21TI); Liquid nitrogen container (CBS model: 2001); Low speed centrifuge (Anting Scientific Instrument Factory, Shanghai's model: KA-1000); 0.2 μm of filter (MILLIPORE model: SLGP033RB); 10cm culture dish (NEST company), 96 well culture plates (NEST company); Cell counting count board; Centrifuge tube, transfer pipet, Tips are some.
2 experimental techniques
1) ECA cell DMEM+10%FBS in 37 DEG C, 5%CO2 carries out cellar culture (10cm culture dish), when Growth of Cells is to logarithmic phase, collecting cell, discards nutrient solution, PBS fine laundering 3 times, add 3ml0.25% trypsinase-0.04%EDTA, after 37 DEG C of digestion 2min, add 5ml perfect medium neutralization reaction wherein, proceeded in centrifuge tube after piping and druming cell, the centrifugal 5min of 1000rpm, adjustment concentration of cell suspension 3 × 104/ml.
2) enter in 96 well culture plates by cell kind, every hole adds cell suspension 180 μ l, and culture plate puts into cell culture incubator (37 DEG C, 5%CO2) cellar culture.
3) according to cell growth status, generally grow to 50%-70%, add to obtain raw cook solution, continue to cultivate 24h.
4) add 20 μ lMTT solution (5mg/ml, i.e. 0.5%MTT) after 24h, continue to cultivate 4h.
5) after 4h, buckle method removes supernatant, pats dry gently with thieving paper, and every hole adds 200 μ l dimethyl sulfoxide (DMSO), puts low-speed oscillation 10min on shaking table, crystallisate is fully dissolved.The light absorption value in each hole is measured at enzyme-linked immunosorbent assay instrument 490nm place.
6) background (do not add cell, only add nutrient solution) is set, control wells (the medicine dissolution medium of cell, same concentrations, nutrient solution, MTT, dimethyl sulfoxide (DMSO)) simultaneously, often organizes the multiple hole of setting 6.
7) result represents with the inhibiting rate of medicine to cell:
Cell proliferation inhibiting rate (%)=(control wells OD value-dosing holes OD value)/control wells OD value × 100%.Experiment repetition 3 times.
3 statistical treatments
Adopt the correlation analysis in MicrosoftExcel2003 software and Studentt inspection, data represent with mean ± S.D..
4 experimental results
Statistical result showed after mtt assay experiment, compare with control group, when dosage reaches 5mg/ml, to ECA cell inhibitory effect variant (P<0.05), dosage this difference when 10mg/ml has significance (P<0.01), has pole significant difference (P<0.001) when dosage reaches 15-20mg/ml.
Table 1 rographolide grinds the impact of ECA cell inhibitory effect
(X ± SD)
Note: compare with control group, * P<0.05; * P<0.01
5 experiment conclusion
Rographolide can suppress ECA cell proliferation, and reduce the Growth of Cells number of ECA cell, this effect is dose-dependently.
Embodiment 5: on the pharmacodynamic study of the impact of the bone density of laboratory animal
1 sample preparation
Rographolide of the present invention: prepare by embodiment 3 method.Precious strong SHENGU JIAONANG, Shandong meeting elixir industry Group Co., Ltd, lot number: 20120711.Tretinoin sheet, Shandong Liang Fu pharmaceutical Co. Ltd, lot number: 130120
2 animals
Wistar rat, male, body weight 180-240g, Shanghai Slac Experimental Animal Co., Ltd. provides.
The foundation of 3 experiment groupings and model rat with osteoporosis
Get normal Wistar rats 30 and be divided into 5 groups at random, get wherein 6 for NS group; Get wherein 6 as model group, press tretinoin 70mg/kg gavage two weeks merely; Get wherein 6 precious strong SHENGU JIAONANG control groups of conduct; Remaining 12 rats are as high low dose group of the present invention.Gavage corresponding medicine while gavaging tretinoin as treasure's male SHENGU JIAONANG control group and high and low dose group of the present invention by the dosage of 70mg/kg, withdraw tretinoin after gavaging two weeks and continue two weeks mensuration indices.Rat sacrificed by decapitation, gets its femur by group arrangement, uses X-line mammary gland pick up camera production mating plate, with black and white densometer on the ectotrochanter of femur, femoral head, neck of femur, intertrochanteric line and under intertrochanteric line 5 positions fixed points measure and record bone density value.
The present invention is on the impact of rat bone density
Note: compare with model group, * P<0.05; * P<0.01
4 experimental results
The present invention compares with model group, has significant difference, illustrates that the present invention can improve bone density, treatment fracture, and effect is better than precious strong SHENGU JIAONANG, can be used for preparing the application increased in bone density medicine.
Claims (2)
1. rographolide increases the application in bone density medicine in preparation, it is characterized in that the extraction step of rographolide is: get Herba Andrographis, join CO
2in supercritical extraction device, ethanol is as entrainment agent, and the volume percent that entrainment agent accounts for total extraction solvent is 4-6%, extracting pressure 15-30MPa, temperature 30-50 DEG C, CO
2flow 1-3m1/g crude drug min, extraction time 150-180min, obtains supercritical extract, add 70% ethanol, drop in microwave extracting apparatus and carry out microwave extracting, extraction power 400-600W, extract 2 times, each 4-8 minute, the amount of 70% ethanol at every turn added is 2 times of medicinal material weight, obtain extraction liquid, concentrated, be added on D101 macroporous adsorptive resins, 50% ethanol elution, collects 5 times amount column volume elutriants, decompression recycling ethanol, concentrated and dry, obtain andrographolide extract.
2. a kind of rographolide increases the application in bone density medicine in preparation according to claim 1, CO described in the extracting method that it is characterized in that rographolide
2the volume percent that supercritical extraction entrainment agent accounts for total extraction solvent is 5%, described CO
2the extracting pressure 20MPa of supercritical extraction, temperature 40 DEG C, CO
2flow 2ml/g crude drug min, extraction time 160min, described microwave extracting power 500W, each extraction 6 minutes.
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| CN105055805A (en) * | 2015-09-06 | 2015-11-18 | 南京正亮医药科技有限公司 | Extraction method and application of herbal composition containing herba andrographitis |
| CN111728967A (en) * | 2019-03-25 | 2020-10-02 | 深圳先进技术研究院 | Application of andrographolide in inhibiting osteoclast formation and activating osteoclast |
| CN112979594B (en) * | 2021-02-26 | 2021-12-14 | 南通科鑫超临界设备有限公司 | Supercritical extraction process of andrographolide |
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Non-Patent Citations (2)
| Title |
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| 响应曲面法建立超临界CO 2 萃取结晶穿心莲内酯工艺模型，;张文成等;《农业工程学报》;20070131;第23卷(第1期);第223-228页 * |
| 穿心莲内酯抗肿瘤作用机制的研究进展;亓翠玲等;《中国中药杂志》;20071031;第32卷(第20期);第2095-2097页 * |
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Effective date of registration: 20160317 Address after: 432900, Xiaogan, Hubei province Xiaochang County Industrial Park South Station Road three East extension Applicant after: Hubei Nuo Kete medicine company limited-liability company Address before: Lishui Economic Development Zone, Nanjing City, Jiangsu province 211200 zhe Ning Road No. 369 Applicant before: Nanjing Zhengliang Medical Technology Co.,Ltd. |
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