CN103478733A - Health-care oral liquid and preparation method thereof - Google Patents
Health-care oral liquid and preparation method thereof Download PDFInfo
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- CN103478733A CN103478733A CN201310379724.7A CN201310379724A CN103478733A CN 103478733 A CN103478733 A CN 103478733A CN 201310379724 A CN201310379724 A CN 201310379724A CN 103478733 A CN103478733 A CN 103478733A
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- 239000007788 liquid Substances 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title abstract description 6
- 102000008186 Collagen Human genes 0.000 claims abstract description 51
- 108010035532 Collagen Proteins 0.000 claims abstract description 51
- 229920001436 collagen Polymers 0.000 claims abstract description 51
- 108010010803 Gelatin Proteins 0.000 claims abstract description 40
- 229920000159 gelatin Polymers 0.000 claims abstract description 40
- 239000008273 gelatin Substances 0.000 claims abstract description 40
- 235000019322 gelatine Nutrition 0.000 claims abstract description 40
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 40
- 238000003756 stirring Methods 0.000 claims description 51
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 26
- 108090000790 Enzymes Proteins 0.000 claims description 22
- 102000004190 Enzymes Human genes 0.000 claims description 22
- 229940088598 enzyme Drugs 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 20
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 13
- 229930006000 Sucrose Natural products 0.000 claims description 13
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 13
- 229960002401 calcium lactate Drugs 0.000 claims description 13
- 235000011086 calcium lactate Nutrition 0.000 claims description 13
- 239000001527 calcium lactate Substances 0.000 claims description 13
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 13
- 238000011049 filling Methods 0.000 claims description 13
- 230000036541 health Effects 0.000 claims description 13
- 229940075582 sorbic acid Drugs 0.000 claims description 13
- 235000010199 sorbic acid Nutrition 0.000 claims description 13
- 239000004334 sorbic acid Substances 0.000 claims description 13
- 230000001954 sterilising effect Effects 0.000 claims description 13
- 239000005720 sucrose Substances 0.000 claims description 13
- 229960004793 sucrose Drugs 0.000 claims description 13
- 239000003814 drug Substances 0.000 claims description 11
- 229940079593 drug Drugs 0.000 claims description 9
- 239000004365 Protease Substances 0.000 claims description 5
- 229940055729 papain Drugs 0.000 claims description 5
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 241000251511 Holothuroidea Species 0.000 claims description 3
- 102000005600 Cathepsins Human genes 0.000 claims description 2
- 108010084457 Cathepsins Proteins 0.000 claims description 2
- 108090000526 Papain Proteins 0.000 claims description 2
- 102000057297 Pepsin A Human genes 0.000 claims description 2
- 108090000284 Pepsin A Proteins 0.000 claims description 2
- 102000005158 Subtilisins Human genes 0.000 claims description 2
- 108010056079 Subtilisins Proteins 0.000 claims description 2
- 235000019834 papain Nutrition 0.000 claims description 2
- 229940111202 pepsin Drugs 0.000 claims description 2
- -1 trypsase Proteins 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 7
- 230000037182 bone density Effects 0.000 abstract description 5
- 230000036039 immunity Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 22
- 239000000047 product Substances 0.000 description 11
- 230000007062 hydrolysis Effects 0.000 description 9
- 238000006460 hydrolysis reaction Methods 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 238000010998 test method Methods 0.000 description 7
- 239000000706 filtrate Substances 0.000 description 5
- 238000000108 ultra-filtration Methods 0.000 description 5
- 239000003292 glue Substances 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 210000000988 bone and bone Anatomy 0.000 description 3
- 229940098773 bovine serum albumin Drugs 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000001976 enzyme digestion Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 208000036487 Arthropathies Diseases 0.000 description 2
- 208000012659 Joint disease Diseases 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 210000000845 cartilage Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000001172 regenerating effect Effects 0.000 description 2
- 229930002330 retinoic acid Natural products 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- YVNQAIFQFWTPLQ-UHFFFAOYSA-O [4-[[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylidene]-3-methylcyclohexa-2,5-dien-1-ylidene]-ethyl-[(3-sulfophenyl)methyl]azanium Chemical compound C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C(=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C)C=2C(=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S(O)(=O)=O)C)C=C1 YVNQAIFQFWTPLQ-UHFFFAOYSA-O 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 210000000527 greater trochanter Anatomy 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000000515 tooth Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides health-care oral liquid and a preparation method thereof. The oral liquid is prepared from donkey-hide gelatin and collagen. The oral liquid can improve immunity, improve skin moisture and increase bone density. Compared with the prior art, the oral liquid has the advantages of simplicity in compatibility, convenience in use and good treatment effect. Moreover, a reasonable and feasible preparation method is provided.
Description
Technical field
The present invention is a kind of health care oral liquid and method for making thereof, belongs to the technical field of health products.
Background technology
It is protein that 16% left and right is arranged in human body component, and collagen is weight accounting approximately 30% to 40% in protein, mainly is present in skin, muscle, bone, tooth, internal organ, blood vessel and eyes place.Though the collagen preparation is many, but effect is undesirable, tracing it to its cause is that prescription has to be optimizedly, and technique has much room for improvement.Prescription, technique determine the quality of product.Conventional collagen extraction process has acid, alkali, enzyme process etc.But the advantage of acid, alkaline process is that technique is simple, but can cause the collagen sex change, and the amine groups molecular structure damages, from the tripeptides structure that is applicable to absorption of human body, to be entangled with as mixed and disorderly peptide chain structure, absorptivity descends, and product function reduces.Enzyme method technique is current ideal and safe technology, can saboteur's structure, but the kind of enzyme and proportioning directly affect the effect of product.
In view of such circumstances, developing the preparation that is rich in collagen that a kind of curative effect is stable has just become people to be badly in need of the thing solved.
Summary of the invention
The object of the invention is to: a kind of health care oral liquid and method for making thereof are provided; The present invention is directed to prior art, bulk drug is made to modern preparation, not only taking convenience, and craft science is reasonable, can better retain effective ingredient, and therapeutic action is good, good market prospects.
The present invention forms like this: described health care oral liquid is that be made donkey-hide gelatin 1-5 part, collagen 1-5 part of the bulk drug by following weight portion is made.Be preferably: 1 part, 1 part, donkey-hide gelatin, collagen are made.Described collagen is also to replace with fish scale collagen, holothurian collagen, pigskin collagen.The method for making of described health care oral liquid: get donkey-hide gelatin, add 10-40 times of water 70-90 ℃ stirring and dissolving; Add collagen, the adjustment solution temperature is 36-42 ℃, and the adjustment pH is 3-9, adds the complex enzyme of 0.1-1%, stirs 1-5h; Add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.Be preferably: get donkey-hide gelatin, add 80 ℃ of stirring and dissolving of 25 times of water; Add collagen, adjusting solution temperature is 40 ℃, and adjusting pH is 5 ~ 7, adds 0.4% complex enzyme, stirs 3h; Add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.Described complex enzyme is in pepsin, trypsase, cathepsin, papain and subtilopeptidase A two kinds or several.Preferred complex enzyme is preferably pepsin-trypsase of mass ratio 1:1:1:1:1-cathepsin-papain-subtilopeptidase A.
The present invention writes out a prescription rationally, and craft science can better retain effective ingredient, and therapeutic action is good.This product is rich in collagen, can binding immunoassay albumen, resist, kill external foreign matter, and allow immunity function promote, build up resistance.And collagen is also skin chief component composition, account for more than 75% of dermal layer of the skin, this product can improve moisture of skin, make skin moist, smooth, be full of elasticity.Moreover, this product can also increase bone density, promotes regenerating bone or cartilage, prevents and improves arthropathy.Donkey-hide gelatin contains collagen, and this is the important composition composition of human body cartilaginous tissue just, and donkey-hide gelatin can also promote the absorption of calcium, stores, and enriches blood, and fluid infusion, therefore can effectively protect and, for the human body cartilaginous tissue provides enough nutrition, extend its aging rate.
The applicant finds that in research process the collagen product of the extraction under mixed enzyme solution technique is than the better dissolubility of having of independent enzymolysis, and the viscosity of the aqueous solution is lower simultaneously, and molecular weight, below 3000 dalton, has good absorptivity.Adopt prescription of the present invention and technique, the product obtained is evident in efficacy, not only can strengthen immunity, can also increase bone density, promotes regenerating bone or cartilage, prevents and improves arthropathy.
The applicant adopts orthogonal design to carry out the screening test of process conditions, for from the mixed enzymolysis effective component extracting, providing scientific and rational theoretical foundation.By relatively finding, with hydrolysis prods respectively, to compare, this product has better curative effect.
1, technical study
Content assaying method: calibration curve: get respectively six, 10ml test tube, wherein a test tube adds 1ml distilled water to do blank, and all the other 5 add respectively the concentration of different volumes is 100ug/ml bovine serum albumin(BSA) titer, and supplementing water is to 1ml.Then every test tube adds 5ml Coomassie brilliant blue G-250 reagent, shakes up and places 5min, measures absorbance at the 595nm place with ultraviolet-visible spectrophotometer.Take A595 as ordinate, and the quantity of bovine serum albumin(BSA) (ug) is abscissa drawing standard curve.Detection method: draw in the solution 0.5ml test tube of falling 10ml to be measured, supplementing water is to 1ml, and retinue be take distilled water as blank.Then add 5ml coomassie G-250 reagent, after shaking up placement 5min, measure absorbance at the 595nm place with ultraviolet-visible spectrophotometer.Check in from calibration curve the quantity that is equivalent to bovine serum albumin(BSA) by the trap recorded, thus the evaluation and test Degree of Enzymatic Hydrolysis.
(1) donkey-hide gelatin dissolves the amount of water investigation
Test method: take donkey-hide gelatin appropriate, add different amount pure water, the heating stirring and dissolving, investigate the glue state.
From the state of above-mentioned glue, adopt 10 ~ 40 times of water-soluble solution gained glue states moderate.5 times of water-soluble solutions are more difficult, surpass 40 too lowly by the gained strength of fluid, therefore determine that amount of water is 10 ~ 40 times, and wherein optimal conditions is 25 times.
(2) the donkey-hide gelatin solution temperature is investigated
Test method: take donkey-hide gelatin appropriate, add 25 times of water, the heating stirring and dissolving, investigate glue state and thawing time.
Can find out from top data, dissolve required time in minimizing along with temperature raises, but temperature be controlled at 60 ℃, mixing time is obviously partially long, and select 70 ~ 90 ℃ can obviously shorten dissolution time, between dominant area, wherein to be 80 ℃ be optimal selection to solution temperature.
(3) hydrolysis temperature is investigated
Test method: donkey-hide gelatin is appropriate, totally 6 parts, add respectively 80 ℃ of stirring and dissolving of 25 times of water, add collagen, adjust solution temperature, adjusting pH is 7, adds 1% complex enzyme, stirs 3h, filter, the ultrafiltration post that filtrate is 30KD and 3KD by the molecular weight cutoff value respectively, adjusting to liquor strength is 0.6g crude drug/ml, measures content.
From the above results, can find out, hydrolysis temperature is in 36 ~ 42 ℃ of intervals, and it is not too greatly that the peptide concentration after enzymolysis changes, but higher than 42 ℃ with lower than 36 ℃, significant change all occurs in peptide concentration, therefore determines that the hydrolysis temperature scope is 36 ~ 42 ℃, and wherein best hydrolysis temperature is 40 ℃.
(4) complex enzyme kind screening
Test method: donkey-hide gelatin is appropriate, totally 6 parts, add respectively 80 ℃ of stirring and dissolving of 25 times of water, add collagen, adjusting solution temperature is 40 ℃, adjusting pH is 7, adds 1% variety classes enzyme, stirs 3h, filter, the ultrafiltration post that filtrate is 30KD and 3KD by the molecular weight cutoff value respectively, adjusting to liquor strength is 0.6g crude drug/ml, measures content.
Can find out from the above results, select above-mentioned various enzyme, can carry out good enzyme digestion reaction, wherein with the effect optimum of complex enzyme.
(5) mixing time is investigated
Test method: donkey-hide gelatin is appropriate, totally 6 parts, add respectively 80 ℃ of stirring and dissolving of 25 times of water, add collagen, adjusting solution temperature is 40 ℃, adjusting pH is 7, adds 1% complex enzyme, stirs different time, filter, the ultrafiltration post that filtrate is 30KD and 3KD by the molecular weight cutoff value respectively, adjusting to liquor strength is 0.6g crude drug/ml, measures content.
From top result, can find out, enzymolysis is after 0.5 hour, and peptide concentration is on the low side, but can obtain hydrolysis result preferably from surpassing 1 hour, and wherein, when enzyme digestion reaction reaches 3 hours, enzyme digestion reaction reaches peak.
(6) pH is investigated
Test method: donkey-hide gelatin is appropriate, totally 6 parts, add respectively 80 ℃ of stirring and dissolving of 25 times of water, add collagen, adjusting solution temperature is 40 ℃, adjust pH, add 1% complex enzyme, stir 3h, filter, the ultrafiltration post that filtrate is 30KD and 3KD by the molecular weight cutoff value respectively, adjusting to liquor strength is 0.6g crude drug/ml, measures content.
From top result of the test, can find out, the pH value is too high and too low all influential to the enzymolysis result of the test, and control the pH value, can obtain good hydrolysis result between 3 ~ 9, wherein take pH=5 ~ 7 as optimum.
(7) the complex enzyme consumption is investigated
Test method: donkey-hide gelatin is appropriate, totally 6 parts, add respectively 80 ℃ of stirring and dissolving of 25 times of water, add collagen, adjusting solution temperature is 40 ℃, adjusting pH is 7, adds the complex enzyme of different amounts, stirs 3h, filter, the ultrafiltration post that filtrate is 30KD and 3KD by the molecular weight cutoff value respectively, adjusting to liquor strength is 0.6g crude drug/ml, measures content.
From top result of the test, can find out, when enzyme dosage is 0.05%, consumption is on the low side, and consumption hydrolysis result between 0.1 ~ 1% is all better, and can reach preferably hydrolysis result when consumption reaches 0.4%.
2, pharmacodynamic experiment
By the rat random packet, wherein osteoporosis model group, oral liquid 1(donkey-hide gelatin: collagen=1:1, mixed enzymolysis) group (high dose: clinical RD 20 times), oral liquid 2(donkey-hide gelatin: collagen=1:1, independent enzymolysis) group (high dose: clinical RD 20 times) all awards vitamin A acid 70mg/ day, gavage, the medicine usage is identical.After 2 weeks, withdraw vitamin A acid, continue to give medicine 2 weeks, measure bone density value.
The bone density value measurement result
Group femoral head neck of femur greater trochanter
Control group 0.58 ± 0.04 0.54 ± 0.02 0.60 ± 0.05
Model group 0.45 ± 0.01 0.55 ± 0.03 0.46 ± 0.02
Oral liquid 1 group 0.52 ± 0.02 0.53 ± 0.04 0.54 ± 0.01
Oral liquid 2 group 0.49 ± 0.06 0.50 ± 0.02 0.49 ± 0.02
Result shows, the product curative effect ideal of mixed enzymolysis.
Concrete embodiment:
Embodiment 1: 1 part, donkey-hide gelatin, 1 part of collagen
Donkey-hide gelatin adds 80 ℃ of stirring and dissolving of 25 times of water in right amount, add collagen, adjusting solution temperature is 40 ℃, and adjusting pH is 5.5, adds pepsin-trypsase of 0.4% mass ratio 1:1:1:1:1-cathepsin-papain-subtilopeptidase A, stir 3h, add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.Each 10-20ml, every day 2-3 time.
Embodiment 2: 1 part, donkey-hide gelatin, 5 parts of collagens
Donkey-hide gelatin adds 90 ℃ of stirring and dissolving of 40 times of water in right amount, add collagen, adjusting solution temperature is 42 ℃, and adjusting pH is 9, adds cathepsin-papain of 1% mass ratio 2:3:1-subtilopeptidase A, stir 5h, add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.
Embodiment 3: 5 parts, donkey-hide gelatin, 1 part of collagen
Donkey-hide gelatin adds 70 ℃ of stirring and dissolving of 10 times of water in right amount, add collagen, adjusting solution temperature is 36 ℃, and adjusting pH is 3, adds pepsin-trypsase of 0.1% mass ratio 1:2, stir 1h, add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling and get final product.
Embodiment 4: 2 parts, donkey-hide gelatin, 3 parts of fish scale collagens
Donkey-hide gelatin adds 85 ℃ of stirring and dissolving of 20 times of water in right amount, add collagen, adjusting solution temperature is 40 ℃, and adjusting pH is 8, adds pepsin-papain of 0.6% mass ratio 1:2:1-subtilopeptidase A, stir 2h, add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.
Embodiment 5: 2 parts, donkey-hide gelatin, 3 parts of pigskin collagens
Donkey-hide gelatin adds 85 ℃ of stirring and dissolving of 20 times of water in right amount, add collagen, adjusting solution temperature is 40 ℃, and adjusting pH is 8, adds pepsin-trypsase of 0.6% mass ratio 3:1:1:1-cathepsin-papain, stir 2h, add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.
Embodiment 6: 5 parts, donkey-hide gelatin, 1 part of holothurian collagen
Donkey-hide gelatin adds 80 ℃ of stirring and dissolving of 25 times of water in right amount, add collagen, adjusting solution temperature is 40 ℃, and adjusting pH is 5.5, adds pepsin-trypsase of 0.4% mass ratio 1:1:1:1-cathepsin-subtilopeptidase A, stir 3h, add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.
Embodiment 7: 1 part, donkey-hide gelatin, 1 part of collagen
Donkey-hide gelatin adds 80 ℃ of stirring and dissolving of 15 times of water in right amount, add collagen, adjusting solution temperature is 40 ℃, and adjusting pH is 5.5, adds pepsin-trypsase of 0.4% mass ratio 2:1:1-cathepsin, stir 3h, add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.
Embodiment 8: 1 part, donkey-hide gelatin, 1 part of collagen
Donkey-hide gelatin adds 80 ℃ of stirring and dissolving of 20 times of water in right amount, add collagen, adjusting solution temperature is 40 ℃, and adjusting pH is 5.5, adds pepsin-trypsase of 0.3% mass ratio 1:2:1-cathepsin, stir 3h, add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.
Embodiment 9: 1 part, donkey-hide gelatin, 1 part of collagen
Donkey-hide gelatin adds 80 ℃ of stirring and dissolving of 20 times of water in right amount, add collagen, adjusting solution temperature is 40 ℃, and adjusting pH is 5.5, adds pepsin-trypsase of 0.3% mass ratio 1:1:2-cathepsin, stir 3h, add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.
Claims (7)
1. a health care oral liquid, it is characterized in that it is that bulk drug by following weight portion is made: donkey-hide gelatin 1-5 part, collagen 1-5 part are made.
2. according to health care oral liquid claimed in claim 1, it is characterized in that it is that bulk drug by following weight portion is made: 1 part, 1 part, donkey-hide gelatin, collagen are made.
3. according to the described health care oral liquid of claim 1 or 2, it is characterized in that: described collagen is also to replace with fish scale collagen, holothurian collagen, pigskin collagen.
4. according to the method for making of claim 1 or 2 described health care oral liquid, it is characterized in that: get donkey-hide gelatin, add 10-40 times of water 70-90 ℃ stirring and dissolving; Add collagen, the adjustment solution temperature is 36-42 ℃, and the adjustment pH is 3-9, adds the complex enzyme of 0.1-1%, stirs 1-5h; Add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.
5. according to the method for making of health care oral liquid claimed in claim 4, it is characterized in that: get donkey-hide gelatin, add 80 ℃ of stirring and dissolving of 25 times of water; Add collagen, adjusting solution temperature is 40 ℃, and adjusting pH is 5 ~ 7, adds 0.4% complex enzyme, stirs 3h; Add again sucrose and calcium lactate, sorbic acid, stirring and dissolving, constant volume, sterilizing, filling.
6. according to the method for making of claim 4 or 5 described health care oral liquids, it is characterized in that: described complex enzyme is in pepsin, trypsase, cathepsin, papain and subtilopeptidase A two kinds or several.
7. according to the method for making of health care oral liquid claimed in claim 6, it is characterized in that: described complex enzyme is preferably pepsin-trypsase of mass ratio 1:1:1:1:1-cathepsin-papain-subtilopeptidase A.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104397723A (en) * | 2014-11-21 | 2015-03-11 | 贵州神奇药物研究院 | Product for improving immunity and preparation method of product |
| CN106177922A (en) * | 2016-09-07 | 2016-12-07 | 海南金海岸生物技术研究所 | A kind of composite collagen gel increasing bone density, prevention senile osteoporosis and preparation method thereof |
| CN108208627A (en) * | 2017-12-31 | 2018-06-29 | 山东禹泽医药科技有限公司 | The formula and preparation process of a kind of vinegar egg juice |
-
2013
- 2013-08-28 CN CN201310379724.7A patent/CN103478733A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104397723A (en) * | 2014-11-21 | 2015-03-11 | 贵州神奇药物研究院 | Product for improving immunity and preparation method of product |
| CN106177922A (en) * | 2016-09-07 | 2016-12-07 | 海南金海岸生物技术研究所 | A kind of composite collagen gel increasing bone density, prevention senile osteoporosis and preparation method thereof |
| CN108208627A (en) * | 2017-12-31 | 2018-06-29 | 山东禹泽医药科技有限公司 | The formula and preparation process of a kind of vinegar egg juice |
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Application publication date: 20140101 |