CN103478707B - Insulin-like growth factor-I sustained release tablet and preparation method thereof - Google Patents
Insulin-like growth factor-I sustained release tablet and preparation method thereof Download PDFInfo
- Publication number
- CN103478707B CN103478707B CN201310371772.1A CN201310371772A CN103478707B CN 103478707 B CN103478707 B CN 103478707B CN 201310371772 A CN201310371772 A CN 201310371772A CN 103478707 B CN103478707 B CN 103478707B
- Authority
- CN
- China
- Prior art keywords
- insulin
- growth factor
- casein
- soybean protein
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 title claims abstract description 40
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 title claims abstract description 40
- 239000007939 sustained release tablet Substances 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims description 29
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 54
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims abstract description 46
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 35
- 244000068988 Glycine max Species 0.000 claims abstract description 34
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 34
- 239000005018 casein Substances 0.000 claims abstract description 32
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims abstract description 32
- 235000021240 caseins Nutrition 0.000 claims abstract description 32
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims abstract description 23
- 229960005305 adenosine Drugs 0.000 claims abstract description 23
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims abstract description 17
- 102000008186 Collagen Human genes 0.000 claims abstract description 17
- 108010035532 Collagen Proteins 0.000 claims abstract description 17
- 235000012000 cholesterol Nutrition 0.000 claims abstract description 17
- 229920001436 collagen Polymers 0.000 claims abstract description 17
- 229940067606 lecithin Drugs 0.000 claims abstract description 17
- 235000010445 lecithin Nutrition 0.000 claims abstract description 17
- 239000000787 lecithin Substances 0.000 claims abstract description 17
- 238000002156 mixing Methods 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 11
- 108010073771 Soybean Proteins Proteins 0.000 claims description 52
- 235000019710 soybean protein Nutrition 0.000 claims description 52
- 239000002502 liposome Substances 0.000 claims description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 33
- 239000004365 Protease Substances 0.000 claims description 28
- 230000007062 hydrolysis Effects 0.000 claims description 27
- 238000006460 hydrolysis reaction Methods 0.000 claims description 27
- 102000048143 Insulin-Like Growth Factor II Human genes 0.000 claims description 24
- 108090001117 Insulin-Like Growth Factor II Proteins 0.000 claims description 24
- 239000006228 supernatant Substances 0.000 claims description 18
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 16
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- 229940088598 enzyme Drugs 0.000 claims description 13
- 102000004190 Enzymes Human genes 0.000 claims description 12
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 235000019419 proteases Nutrition 0.000 claims description 10
- 241000228245 Aspergillus niger Species 0.000 claims description 9
- 108010004032 Bromelains Proteins 0.000 claims description 9
- 108090000526 Papain Proteins 0.000 claims description 9
- 102000057297 Pepsin A Human genes 0.000 claims description 9
- 108090000284 Pepsin A Proteins 0.000 claims description 9
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 9
- 235000019835 bromelain Nutrition 0.000 claims description 9
- 235000019834 papain Nutrition 0.000 claims description 9
- 229940055729 papain Drugs 0.000 claims description 9
- 229940111202 pepsin Drugs 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000000748 compression moulding Methods 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 8
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 8
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 8
- 235000019800 disodium phosphate Nutrition 0.000 claims description 8
- 239000012153 distilled water Substances 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 8
- 239000000839 emulsion Substances 0.000 claims description 8
- 238000001976 enzyme digestion Methods 0.000 claims description 8
- 239000012467 final product Substances 0.000 claims description 8
- 230000036571 hydration Effects 0.000 claims description 8
- 238000006703 hydration reaction Methods 0.000 claims description 8
- 230000007246 mechanism Effects 0.000 claims description 8
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 8
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 8
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 8
- 239000001103 potassium chloride Substances 0.000 claims description 8
- 235000011164 potassium chloride Nutrition 0.000 claims description 8
- 238000001556 precipitation Methods 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- -1 8 parts Chemical compound 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 9
- 239000000463 material Substances 0.000 abstract description 2
- 235000019764 Soybean Meal Nutrition 0.000 abstract 2
- 239000004455 soybean meal Substances 0.000 abstract 2
- 238000013268 sustained release Methods 0.000 abstract 1
- 239000012730 sustained-release form Substances 0.000 abstract 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 18
- 239000000243 solution Substances 0.000 description 13
- 210000002540 macrophage Anatomy 0.000 description 10
- 241000287828 Gallus gallus Species 0.000 description 9
- 230000000242 pagocytic effect Effects 0.000 description 9
- 102000035195 Peptidases Human genes 0.000 description 8
- 210000003743 erythrocyte Anatomy 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 6
- 230000036039 immunity Effects 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 239000003228 hemolysin Substances 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 241000699670 Mus sp. Species 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 239000002504 physiological saline solution Substances 0.000 description 4
- 108010006464 Hemolysin Proteins Proteins 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 235000014590 basal diet Nutrition 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 229930024421 Adenine Natural products 0.000 description 2
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102100037852 Insulin-like growth factor I Human genes 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 229960000643 adenine Drugs 0.000 description 2
- UDMBCSSLTHHNCD-KQYNXXCUSA-N adenosine 5'-monophosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N aldehydo-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- 210000003567 ascitic fluid Anatomy 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 1
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 206010057249 Phagocytosis Diseases 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- 235000008452 baby food Nutrition 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000001045 blue dye Substances 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 210000004351 coronary vessel Anatomy 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 235000019700 dicalcium phosphate Nutrition 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000916 dilatatory effect Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000000774 hypoallergenic effect Effects 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000008782 phagocytosis Effects 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000011496 sports drink Nutrition 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 229940124549 vasodilator Drugs 0.000 description 1
- 239000003071 vasodilator agent Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses an insulin-like growth factor-I sustained release tablet. The sustained release tablet is prepared from an insulin-like growth factor-I, casein, adenosine, soybean meal, lecithin, cholesterol and collagen. The sustained release tablet is prepared by firstly extracting soybean peptide from the soybean meal, secondly, preparing the raw materials of the insulin-like growth factor-I, the casein and the like into lipidosome, and uniformly mixing the materials with other raw materials and preparing so as to obtain the sustained release tablet. The sustained release tablet has the advantages that the components are scientific, the organism immune is effectively improved, the quality is stable, a good sustained release effect is achieved, the bioavailability is high, and the like.
Description
Technical field
The present invention relates to field of health care products, be specifically related to insulin-like growth factor-Ⅰ sustained release tablets.
Background technology
Insulin-like growth factor-Ⅰ (IGF-I) is a kind of activated protein peptide material, and it has: 1. hypoglycemic; 2. reducing blood lipid; 3. vasodilator; 4. promote that the anabolism of bone keeps its normal configuration function; 5. growth promotion; 6. short Cell Differentiation; 7. effect such as wound repair.But independent oral IGF-I is but difficult to be absorbed, 10% not digested liquid of only having an appointment decomposes and is substantially absorbed in its entirety, but takes with casein simultaneously, and absorbed intact rate can reach 46%.Casein can promote that human body is for the absorption of IGF-I.
Soybean Peptide is the mixture of the peptide chain be made up of different aminoacids that soybean protein obtains through acidolysis or enzymolysis, based on small-molecular peptides, also containing a small amount of free amino acid, carbohydrate, the large composition such as molecular peptide, inorganic salts.Soybean Peptide have soluble in water, without albuminous degeneration, the physicochemical property such as heating is not solidified, acidity does not precipitate, good fluidity, emulsibility are low, free from beany flavor.Soybean Peptide has absorbability easy to digest, promote Organism immunoregulation, amino acid composition rationally, hypoallergenic and low antigenicity, antioxidation, the synthesis of promotion protein, promote the advantages such as mineral absorption.As a kind of novel functional activity factor, soya-bean polypeptides has a wide range of applications in nutrition remedy diet, functional food, health food, baby food, fermented food and strengthening sports drink.
Adenosine refers to the compound be formed by connecting by β glycosidic bond by the N-9 of adenine and the C-1 of D-ribose, for white crystalline powder, adenosine is a kind of endogenous nucleoside spreading all over human body cell, directly can enter cardiac muscle and generate adenylate through phosphorylation, participate in energy metabolism of myocardial, also participate in coronary artery dilating blood vessel simultaneously, increase CBF.Adenosine to cardiovascular system unify human body other systems many and organize and all have physiological action.Adenosine is the important intermediate for the synthesis of atriphos, adenine, adenylate, arabinosy ladenosine.
Along with people's living standard improves, the attention degree of health is grown with each passing day, though market health products are various in style, but quality and effect uneven, develop a kind of components compatibility science, effectively can improve immunity of organism, and steady quality, there is good slow release effect, health products that bioavilability is high have broad mass market prospect.
Summary of the invention
The present invention is intended to solve prior art above shortcomings, and object is to provide a kind of scientific formulation, has good biological availability, can improve the insulin-like growth factor-Ⅰ sustained release tablets of immunity of organism.
It is rigorous that another object of the present invention is to provide a kind of technique, is applicable to the preparation method of the insulin-like growth factor-Ⅰ sustained release tablets of industrialization promotion.
The technical scheme realizing above-mentioned purpose is:
Insulin-like growth factor-Ⅰ sustained release tablets, the medicament be made up of the raw material of following weight proportioning: insulin-like growth factor-Ⅰ 20-26 part, casein 14-17 part, adenosine 8-12 part, soybean protein 42-54 part, lecithin 24-32 part, cholesterol 6-10 part, collagen 3-6 part;
Concrete preparation method is as follows:
The preparation of Soybean Peptide: get soybean protein and mix in soybean protein quality 5-7 water doubly, put into retort pretreatment 15min under pressure 0.08Mpa, after its cooling, pour enzyme digestion reaction still into, control temperature 55-65 DEG C, adjust ph is to 8.0-8.5, 0.005-0.008g hydrolysis by novo 30-60min is added in every gram of soybean protein, regulate pH value to 7.0, 0.004-0.007g papain is added successively in every gram of soybean protein, 0.006-0.009g bromelain, 0.008-0.012g neutral proteinase hydrolysis 60-90min, regulate pH value to 2.3-2.8, 0.005-0.008g aspergillus niger protease is added successively in every gram of soybean protein, 0.003-0.005g pepsin hydrolysis 3.5-5.5h, under 300-500r/min mixing speed, 90 DEG C of water-baths are gone out enzyme 30 min, hydrolyzate in centrifugal precipitation mechanism to get supernatant after centrifugal 20 min of 2000 r/min rotating speed, supernatant is spray-dried that Soybean Peptide is for subsequent use,
PBS: take sodium chloride 8g, potassium chloride 0.2g, sodium hydrogen phosphate 3.63g, potassium dihydrogen phosphate 0.24g, is dissolved in 900ml distilled water, adjusts pH value to 7.4, adds water and be settled to 1L, obtain PBS for subsequent use;
The preparation of insulin-like growth factor-Ⅱ casein liposome: lecithin, cholesterol, collagen are mixed, the volume fraction being dissolved in three gross mass 8-14 times amount is in the ethanolic solution of 80-95%, separately gets insulin-like growth factor-Ⅰ, casein, Soybean Peptide be dissolved in 10-14 part PBS; After two solution are fully mixed, 50-60 DEG C of water bath sonicator 5-10min, form stable W/0 type emulsion, 25 DEG C of removal of solvent under reduced pressure in Rotary Evaporators, after rotary becomes colloidal state, add 32-38 part PBS, rotate hydration under normal temperature, namely obtain milky liposome turbid liquor, by liposome turbid liquor in-60 DEG C of snap frozen 12h,-50 DEG C of dry 24h in vacuum freeze drier, obtain insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome for subsequent use;
After insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome and adenosine mixing, compression molding and get final product.
The present invention also according to actual production demand, can make other formulations such as capsule, oral liquid.
Wherein, insulin-like growth factor-Ⅰ is purchased from Beijing Duanmu Sheng Da biotechnology Co., Ltd; Casein is purchased from Huaan, Gansu biotechnology group; Adenosine is purchased from Shaanxi Sen Fu high-tech Industrial Co., Ltd.; Soybean protein is purchased from Shandong soybean cultivars albumen Co., Ltd; Alkali protease, papain, bromelain, neutral proteinase are purchased from Pangbo Bioengineering Co Ltd, Nanning; Aspergillus niger protease is purchased from Zaozhuang Jienuo Enzyme Co., Ltd.; Pepsin is purchased from Yuan Ju bio tech ltd, Shanghai.
Principle active component of the present invention is IGF (IGF-I), casein, Soybean Peptide, adenosine, and wherein casein can improve the absorption of human body to IGF-I; Soybean Peptide is that soybean protein is hydrolyzed obtained through complex enzyme stage by stage, shortcoming that combined-enzyme method preparation technology can overcome that the degree of hydrolysis that single enzymatic hydrolysis exists is not high, polypeptide yield is low etc., it can increase protein catalytic site, effectively soybean protein is resolved into the low Small molecular Soybean Peptide of molecular weight, but need the factor of consideration also complicated than single enzymolysis in multienzyme composite hydrolysis process conditions, except the conventional factors such as hydrolysis time, pH, temperature, the factors such as the proportioning also will considering enzyme and the mode that adds enzyme; IGF (IGF-I), casein, Soybean Peptide three are made liposome, product slow releasing in vivo can be made on the one hand, extend action time, bad mouthfeel and the smell of raw material can be covered on the other hand, increase product palatability.Scientific formula of the present invention, effect of each component can reach synergy through rational processes preparation, thus reaches long-time, effective health-care effect improving immunity of organisms.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is described further, to help understanding content of the present invention.
Embodiment 1:
Insulin-like growth factor-Ⅰ sustained release tablets, the medicament be made up of the raw material of following weight proportioning: insulin-like growth factor-Ⅰ 23 parts, casein 15 parts, adenosine 11 parts, soybean protein 49 parts, 28 parts, lecithin, 8 parts, cholesterol, collagen 4 parts;
Concrete preparation method is as follows:
The preparation of Soybean Peptide: getting soybean protein in the water of soybean protein quality 6 times mixes, put into retort pretreatment 15min under pressure 0.08Mpa, after its cooling, pour enzyme digestion reaction still into, control temperature 60 DEG C, adjust ph to 8.3, 0.007g hydrolysis by novo 40min is added in every gram of soybean protein, regulate pH value to 7.0, 0.006g papain is added successively in every gram of soybean protein, 0.007g bromelain, 0.010g neutral proteinase hydrolysis 75min, regulate pH value to 2.5, 0.007g aspergillus niger protease is added successively in every gram of soybean protein, 0.004g pepsin hydrolysis 4.5h, under 420r/min mixing speed, 90 DEG C of water-baths are gone out enzyme 30 min, hydrolyzate in centrifugal precipitation mechanism to get supernatant after centrifugal 20 min of 2000 r/min rotating speed, supernatant is spray-dried that Soybean Peptide is for subsequent use,
PBS: take sodium chloride 8g, potassium chloride 0.2g, sodium hydrogen phosphate 3.63g, potassium dihydrogen phosphate 0.24g, is dissolved in 900ml distilled water, adjusts pH value to 7.4, adds water and be settled to 1L, obtain PBS for subsequent use;
The preparation of insulin-like growth factor-Ⅱ casein liposome: lecithin, cholesterol, collagen are mixed, the volume fraction being dissolved in three's gross mass 12 times amount is in the ethanolic solution of 85%, separately gets insulin-like growth factor-Ⅰ, casein, Soybean Peptide be dissolved in 12 parts of PBSs; After two solution are fully mixed, 55 DEG C of water bath sonicator 8min, form stable W/0 type emulsion, 25 DEG C of removal of solvent under reduced pressure in Rotary Evaporators, after rotary becomes colloidal state, add 35 parts of PBSs, rotate hydration under normal temperature, namely obtain milky liposome turbid liquor, by liposome turbid liquor in-60 DEG C of snap frozen 12h,-50 DEG C of dry 24h in vacuum freeze drier, obtain insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome for subsequent use;
After insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome and adenosine mixing, compression molding and get final product.
Embodiment 2
Insulin-like growth factor-Ⅰ sustained release tablets, the medicament be made up of the raw material of following weight proportioning: insulin-like growth factor-Ⅰ 20 parts, casein 14 parts, adenosine 8 parts, soybean protein 42 parts, 24 parts, lecithin, 6 parts, cholesterol, collagen 3 parts;
Concrete preparation method is as follows:
The preparation of Soybean Peptide: getting soybean protein in the water of soybean protein quality 5 times mixes, put into retort pretreatment 15min under pressure 0.08Mpa, after its cooling, pour enzyme digestion reaction still into, control temperature 55 DEG C, adjust ph to 8.0, 0.005g hydrolysis by novo 30min is added in every gram of soybean protein, regulate pH value to 7.0, 0.004g papain is added successively in every gram of soybean protein, 0.006g bromelain, 0.008g neutral proteinase hydrolysis 60min, regulate pH value to 2.3, 0.005g aspergillus niger protease is added successively in every gram of soybean protein, 0.003g pepsin hydrolysis 3.5h, under 300r/min mixing speed, 90 DEG C of water-baths are gone out enzyme 30 min, hydrolyzate in centrifugal precipitation mechanism to get supernatant after centrifugal 20 min of 2000 r/min rotating speed, supernatant is spray-dried that Soybean Peptide is for subsequent use,
PBS: take sodium chloride 8g, potassium chloride 0.2g, sodium hydrogen phosphate 3.63g, potassium dihydrogen phosphate 0.24g, is dissolved in 900ml distilled water, adjusts pH value to 7.4, adds water and be settled to 1L, obtain PBS for subsequent use;
The preparation of insulin-like growth factor-Ⅱ casein liposome: lecithin, cholesterol, collagen are mixed, the volume fraction being dissolved in three's gross mass 8 times amount is in the ethanolic solution of 80%, separately gets insulin-like growth factor-Ⅰ, casein, Soybean Peptide be dissolved in 10 parts of PBSs; After two solution are fully mixed, 50 DEG C of water bath sonicator 5min, form stable W/0 type emulsion, 25 DEG C of removal of solvent under reduced pressure in Rotary Evaporators, after rotary becomes colloidal state, add 32 parts of PBSs, rotate hydration under normal temperature, namely obtain milky liposome turbid liquor, by liposome turbid liquor in-60 DEG C of snap frozen 12h,-50 DEG C of dry 24h in vacuum freeze drier, obtain insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome for subsequent use;
After insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome and adenosine mixing, compression molding and get final product.
Embodiment 3
Insulin-like growth factor-Ⅰ sustained release tablets, the medicament be made up of the raw material of following weight proportioning: insulin-like growth factor-Ⅰ 26 parts, casein 17 parts, adenosine 12 parts, soybean protein 54 parts, 32 parts, lecithin, 10 parts, cholesterol, collagen 6 parts;
Concrete preparation method is as follows:
The preparation of Soybean Peptide: getting soybean protein in the water of soybean protein quality 7 times mixes, put into retort pretreatment 15min under pressure 0.08Mpa, after its cooling, pour enzyme digestion reaction still into, control temperature 65 DEG C, adjust ph to 8.5, 0.008g hydrolysis by novo 60min is added in every gram of soybean protein, regulate pH value to 7.0, 0.007g papain is added successively in every gram of soybean protein, 0.009g bromelain, 0.012g neutral proteinase hydrolysis 90min, regulate pH value to 2.8, 0.008g aspergillus niger protease is added successively in every gram of soybean protein, 0.005g pepsin hydrolysis 5.5h, under 500r/min mixing speed, 90 DEG C of water-baths are gone out enzyme 30 min, hydrolyzate in centrifugal precipitation mechanism to get supernatant after centrifugal 20 min of 2000 r/min rotating speed, supernatant is spray-dried that Soybean Peptide is for subsequent use,
PBS: take sodium chloride 8g, potassium chloride 0.2g, sodium hydrogen phosphate 3.63g, potassium dihydrogen phosphate 0.24g, is dissolved in 900ml distilled water, adjusts pH value to 7.4, adds water and be settled to 1L, obtain PBS for subsequent use;
The preparation of insulin-like growth factor-Ⅱ casein liposome: lecithin, cholesterol, collagen are mixed, the volume fraction being dissolved in three's gross mass 14 times amount is in the ethanolic solution of 95%, separately gets insulin-like growth factor-Ⅰ, casein, Soybean Peptide be dissolved in 14 parts of PBSs; After two solution are fully mixed, 60 DEG C of water bath sonicator 10min, form stable W/0 type emulsion, 25 DEG C of removal of solvent under reduced pressure in Rotary Evaporators, after rotary becomes colloidal state, add 38 parts of PBSs, rotate hydration under normal temperature, namely obtain milky liposome turbid liquor, by liposome turbid liquor in-60 DEG C of snap frozen 12h,-50 DEG C of dry 24h in vacuum freeze drier, obtain insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome for subsequent use;
After insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome and adenosine mixing, compression molding and get final product.
Embodiment 4
Insulin-like growth factor-Ⅰ sustained release tablets, the medicament be made up of the raw material of following weight proportioning: insulin-like growth factor-Ⅰ 18 parts, casein 12 parts, adenosine 6 parts, soybean protein 50 parts, 20 parts, lecithin, 5 parts, cholesterol, collagen 4 parts;
Concrete preparation method is as follows:
The preparation of Soybean Peptide: getting soybean protein in the water of soybean protein quality 4 times mixes, put into retort pretreatment 15min under pressure 0.08Mpa, after its cooling, pour enzyme digestion reaction still into, control temperature 50 DEG C, adjust ph to 8.0, 0.004g hydrolysis by novo 30min is added in every gram of soybean protein, regulate pH value to 7.0, 0.003g papain is added successively in every gram of soybean protein, 0.005g bromelain, 0.007g neutral proteinase hydrolysis 50min, regulate pH value to 2.1, 0.004g aspergillus niger protease is added successively in every gram of soybean protein, 0.002g pepsin hydrolysis 3h, under 200r/min mixing speed, 90 DEG C of water-baths are gone out enzyme 30 min, hydrolyzate in centrifugal precipitation mechanism to get supernatant after centrifugal 20 min of 2000 r/min rotating speed, supernatant is spray-dried that Soybean Peptide is for subsequent use,
PBS: take sodium chloride 8g, potassium chloride 0.2g, sodium hydrogen phosphate 3.63g, potassium dihydrogen phosphate 0.24g, is dissolved in 900ml distilled water, adjusts pH value to 7.4, adds water and be settled to 1L, obtain PBS for subsequent use;
The preparation of insulin-like growth factor-Ⅱ casein liposome: lecithin, cholesterol, collagen are mixed, the volume fraction being dissolved in three's gross mass 7 times amount is in the ethanolic solution of 70%, separately gets insulin-like growth factor-Ⅰ, casein, Soybean Peptide be dissolved in 8 parts of PBSs; After two solution are fully mixed, 40 DEG C of water bath sonicator 4min, form stable W/0 type emulsion, 25 DEG C of removal of solvent under reduced pressure in Rotary Evaporators, after rotary becomes colloidal state, add 28 parts of PBSs, rotate hydration under normal temperature, namely obtain milky liposome turbid liquor, by liposome turbid liquor in-60 DEG C of snap frozen 12h,-50 DEG C of dry 24h in vacuum freeze drier, obtain insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome for subsequent use;
After insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome and adenosine mixing, compression molding and get final product.
Embodiment 5
Insulin-like growth factor-Ⅰ sustained release tablets, the medicament be made up of the raw material of following weight proportioning: insulin-like growth factor-Ⅰ 28 parts, casein 18 parts, adenosine 13 parts, soybean protein 55 parts, 34 parts, lecithin, 13 parts, cholesterol, collagen 7 parts;
Concrete preparation method is as follows:
The preparation of Soybean Peptide: getting soybean protein in the water of soybean protein quality 8 times mixes, put into retort pretreatment 15min under pressure 0.08Mpa, after its cooling, pour enzyme digestion reaction still into, control temperature 75 DEG C, adjust ph to 8. 5, 0.009g hydrolysis by novo 60min is added in every gram of soybean protein, regulate pH value to 7.0, 0.008g papain is added successively in every gram of soybean protein, 0.010g bromelain, 0.013g neutral proteinase hydrolysis 90min, regulate pH value to 2.9, 0.009g aspergillus niger protease is added successively in every gram of soybean protein, 0.006g pepsin hydrolysis 6h, under 600r/min mixing speed, 90 DEG C of water-baths are gone out enzyme 30 min, hydrolyzate in centrifugal precipitation mechanism to get supernatant after centrifugal 20 min of 2000 r/min rotating speed, supernatant is spray-dried that Soybean Peptide is for subsequent use,
PBS: take sodium chloride 8g, potassium chloride 0.2g, sodium hydrogen phosphate 3.63g, potassium dihydrogen phosphate 0.24g, is dissolved in 900ml distilled water, adjusts pH value to 7.4, adds water and be settled to 1L, obtain PBS for subsequent use;
The preparation of insulin-like growth factor-Ⅱ casein liposome: lecithin, cholesterol, collagen are mixed, be dissolved in the ethanol solution of three's gross mass 15 times amount, separately get insulin-like growth factor-Ⅰ, casein, Soybean Peptide be dissolved in 10-14 part PBS; After two solution are fully mixed, 70 DEG C of water bath sonicator 15min, form stable W/0 type emulsion, 25 DEG C of removal of solvent under reduced pressure in Rotary Evaporators, after rotary becomes colloidal state, add 40 parts of PBSs, rotate hydration under normal temperature, namely obtain milky liposome turbid liquor, by liposome turbid liquor in-60 DEG C of snap frozen 12h,-50 DEG C of dry 24h in vacuum freeze drier, obtain insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome for subsequent use;
After insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome and adenosine mixing, compression molding and get final product.
Test example
Experimental animal: the healthy mice choosing body weight 18-22 g, male and female have concurrently, and mouse basal diet is filled a prescription: corn 45 %, low temperature soy meal 20 %, wheat bran 10 %, imported fish meal 11 %, 7.5 % that crack rice, yeast extract 3 %, calcium monohydrogen phosphate 1 %, stone flour 1 %, salt 0.5 %, compound vitamin many ore deposits 1 %.Test grouping: get mouse 180, be divided into three batches at random, often criticize and be divided into 6 groups at random, often organizes 10.Control group fed basal diet, add product prepared by embodiment 1-5 respectively, and addition is 2 % of daily ration quality in the basal diet of embodiment 1-5 group test mouse.
1, mouse immune shoot formation measures
Test method: first mouse normally raises 30d.For test mice is weighed after 30d, after weighing, cervical dislocation is put to death, and separating spleen, thymus gland, weigh after filter paper suck dry moisture respectively, Computation immunity shoot formation.
Immune Organs Index=immune organ quality (mg)/body weight (g), the results are shown in Table 1:
Table 1 mouse immune shoot formation
As seen from the above table, embodiment group compares with control group, and index and spleen index and thymus index significantly improve.Wherein embodiment 1-3 is obviously better than embodiment 4-5, and embodiment 1 effect is optimum.
2, mouse macrophage phagocytosis test
Test method: second batch mouse, 6% soluble starch solution adds appropriate 3% trypan blue dye liquor, and experiment terminates a few days ago to inject 1 mL, produces more macrophage to stimulate in abdominal cavity.Inject 1% chicken cell suspension 1 mL to after 48 h every mouse peritoneal, after injection, soft belly makes it be uniformly dispersed.After injection chicken red blood cell 30 mim, put to death mouse with cervical dislocation, cut mouse stomach wall open, to Intraperitoneal injection 0.5mL physiological saline, soft abdominal cavity 1 ~ 2mim, peritoneal fluid and physiological saline mixed and flushes out more macrophages, extracting peritoneal fluid with syringe, dripping 2 with on slide, covered, microscopic examination 100 macrophages, record the macrophage number of having engulfed chicken red blood cell and by the chicken red blood cell of macrophage phagocytic sum, the results are shown in Table 2:
Phagocytic rate (%)=(engulfing the macrophage number of the macrophage number/counting of chicken red blood cell) * 100
Phagocytic index=by engulf chicken red blood cell sum/counting macrophage number
The mensuration of table 2 mouse macrophage phagocytic activity
| Group | Quantity (only) | Phagocytic rate (%) | Phagocytic index |
| Blank group | 10 | 36.7±5.21 | 0.420±0.038 |
| Embodiment 1 group | 10 | 47.3±5.34 | 0.512±0.050 |
| Embodiment 2 groups | 10 | 47.0±4.82 | 0.499±0.027 |
| Embodiment 3 groups | 10 | 47.1±5.93 | 0.503±0.043 |
| Embodiment 4 groups | 10 | 41.8±5.07 | 0.431±0.052 |
| Embodiment 5 groups | 10 | 42.3±4.72 | 0.445±0.044 |
Result of the test shows: the present invention can improve the phagocytic activity of mouse to chicken red blood cell, and especially optimum with embodiment 1 effect, phagocytic rate and phagocytic index are significantly better than embodiment 4-5 and blank group, and visible the present invention can improve body non-specific immune function.
3, mice serum hemolysin test
Test method: the 3rd batch of mouse is fed after 14d continuously, each group of mouse peritoneal injects 5% physiological saline chicken erythrocyte suspension 0.2 mL immunity, 7 d after immunity, ether inhalation anesthesia mouse, win eyeball and get blood 1 mL in 1.5 mL centrifuge tubes, centrifugal 20 min of 2000 r/min after standing 30 min, get supernatant and preserve; During use, serum 1 mL of 100 times of dilutions mixes with 10 % complement 0.5 mL, 5% physiological saline chicken erythrocyte suspension 0.5 mL, centrifugal 5 min of 37 DEG C of heat and moisture preserving 30 min, 1000 r/min, gets supernatant and measures absorbance in 540 nm places.The results are shown in Table 3:
Table 3 mice serum hemolysin test
| Group | Quantity (only) | Serum hemolysin (OD value) |
| Blank group | 10 | 0.164±0.051 |
| Embodiment 1 group | 10 | 0.223±0.042 |
| Embodiment 2 groups | 10 | 0.222±0.036 |
| Embodiment 3 groups | 10 | 0.220±0.043 |
| Embodiment 4 groups | 10 | 0.206±0.019 |
| Embodiment 5 groups | 10 | 0.197±0.025 |
Compare with blank group, embodiment 1-5 group serum hemolysin raises, and embodiment 1-3 group and embodiment 4-5 group significant difference, embodiment 1-3 group difference is not remarkable, but embodiment 1 effect is optimum.By the generation of the visible the present invention of result of the test to immune mouse hemolysin, there is obvious excitation, improve the specific immune function of mouse.
Claims (2)
1. insulin-like growth factor-Ⅰ sustained release tablets, is characterized in that: the medicament be made up of the raw material of following weight proportioning: insulin-like growth factor-Ⅰ 20-26 part, casein 14-17 part, adenosine 8-12 part, soybean protein 42-54 part, lecithin 24-32 part, cholesterol 6-10 part, collagen 3-6 part;
Concrete preparation method is as follows:
The preparation of Soybean Peptide: get soybean protein and mix in soybean protein quality 5-7 water doubly, put into retort pretreatment 15min under pressure 0.08Mpa, after its cooling, pour enzyme digestion reaction still into, control temperature 55-65 DEG C, adjust ph is to 8.0-8.5, 0.005-0.008g hydrolysis by novo 30-60min is added in every gram of soybean protein, regulate pH value to 7.0, 0.004-0.007g papain is added successively in every gram of soybean protein, 0.006-0.009g bromelain, 0.008-0.012g neutral proteinase hydrolysis 60-90min, regulate pH value to 2.3-2.8, 0.005-0.008g aspergillus niger protease is added successively in every gram of soybean protein, 0.003-0.005g pepsin hydrolysis 3.5-5.5h, under 300-500r/min mixing speed, 90 DEG C of water-baths are gone out enzyme 30 min, hydrolyzate in centrifugal precipitation mechanism to get supernatant after centrifugal 20 min of 2000 r/min rotating speed, supernatant is spray-dried that Soybean Peptide is for subsequent use,
PBS: take sodium chloride 8g, potassium chloride 0.2g, sodium hydrogen phosphate 3.63g, potassium dihydrogen phosphate 0.24g, is dissolved in 900ml distilled water, adjusts pH value to 7.4, adds water and be settled to 1L, obtain PBS for subsequent use;
The preparation of insulin-like growth factor-Ⅱ casein liposome: lecithin, cholesterol, collagen are mixed, the volume fraction being dissolved in three gross mass 8-14 times amount is in the ethanolic solution of 80-95%, separately gets insulin-like growth factor-Ⅰ, casein, Soybean Peptide be dissolved in 10-14 part PBS; After two solution are fully mixed, 50-60 DEG C of water bath sonicator 5-10min, form stable W/0 type emulsion, 25 DEG C of removal of solvent under reduced pressure in Rotary Evaporators, after rotary becomes colloidal state, add 32-38 part PBS, rotate hydration under normal temperature, namely obtain milky liposome turbid liquor, by liposome turbid liquor in-60 DEG C of snap frozen 12h,-50 DEG C of dry 24h in vacuum freeze drier, obtain insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome for subsequent use;
After insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome and adenosine mixing, compression molding and get final product.
2. insulin-like growth factor-Ⅰ sustained release tablets as claimed in claim 1, is characterized in that: the medicament be made up of the raw material of following weight proportioning: insulin-like growth factor-Ⅰ 23 parts, casein 15 parts, adenosine 11 parts, soybean protein 49 parts, 28 parts, lecithin, 8 parts, cholesterol, collagen 4 parts;
Concrete preparation method is as follows:
The preparation of Soybean Peptide: getting soybean protein in the water of soybean protein quality 6 times mixes, put into retort pretreatment 15min under pressure 0.08Mpa, after its cooling, pour enzyme digestion reaction still into, control temperature 60 DEG C, adjust ph to 8.3, 0.007g hydrolysis by novo 40min is added in every gram of soybean protein, regulate pH value to 7.0, 0.006g papain is added successively in every gram of soybean protein, 0.007g bromelain, 0.010g neutral proteinase hydrolysis 75min, regulate pH value to 2.5, 0.007g aspergillus niger protease is added successively in every gram of soybean protein, 0.004g pepsin hydrolysis 4.5h, under 420r/min mixing speed, 90 DEG C of water-baths are gone out enzyme 30 min, hydrolyzate in centrifugal precipitation mechanism to get supernatant after centrifugal 20 min of 2000 r/min rotating speed, supernatant is spray-dried that Soybean Peptide is for subsequent use,
PBS: take sodium chloride 8g, potassium chloride 0.2g, sodium hydrogen phosphate 3.63g, potassium dihydrogen phosphate 0.24g, is dissolved in 900ml distilled water, adjusts pH value to 7.4, adds water and be settled to 1L, obtain PBS for subsequent use;
The preparation of insulin-like growth factor-Ⅱ casein liposome: lecithin, cholesterol, collagen are mixed, the volume fraction being dissolved in three's gross mass 12 times amount is in the ethanolic solution of 85%, separately gets insulin-like growth factor-Ⅰ, casein, Soybean Peptide be dissolved in 12 parts of PBSs; After two solution are fully mixed, 55 DEG C of water bath sonicator 8min, form stable W/0 type emulsion, 25 DEG C of removal of solvent under reduced pressure in Rotary Evaporators, after rotary becomes colloidal state, add 35 parts of PBSs, rotate hydration under normal temperature, namely obtain milky liposome turbid liquor, by liposome turbid liquor in-60 DEG C of snap frozen 12h,-50 DEG C of dry 24h in vacuum freeze drier, obtain insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome for subsequent use;
After insulin-like growth factor-Ⅱ casein-Soybean Peptide liposome and adenosine mixing, compression molding and get final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310371772.1A CN103478707B (en) | 2013-08-23 | 2013-08-23 | Insulin-like growth factor-I sustained release tablet and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310371772.1A CN103478707B (en) | 2013-08-23 | 2013-08-23 | Insulin-like growth factor-I sustained release tablet and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103478707A CN103478707A (en) | 2014-01-01 |
| CN103478707B true CN103478707B (en) | 2015-04-08 |
Family
ID=49819569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310371772.1A Expired - Fee Related CN103478707B (en) | 2013-08-23 | 2013-08-23 | Insulin-like growth factor-I sustained release tablet and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103478707B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106267165A (en) * | 2016-08-29 | 2017-01-04 | 楼秀余 | HGH and IGF 1 liposome complex capsule and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6306432B1 (en) * | 1997-09-08 | 2001-10-23 | Chiron Corporation | High and low load formulations of IGF-I in multivesicular liposomes |
| CN100350917C (en) * | 1998-04-30 | 2007-11-28 | 纽米库研究澳大利亚控股有限公司 | Food composition and method of using same |
| CN101768618A (en) * | 2010-04-01 | 2010-07-07 | 哈尔滨商业大学 | Method for preparing exorphins from soybean protein isolate by fractional hydrolysis |
| CN101773194A (en) * | 2010-01-15 | 2010-07-14 | 北京华达杰瑞生物技术有限公司 | Method for extracting soy peptide from soybean meals |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH09278670A (en) * | 1995-10-06 | 1997-10-28 | Fujisawa Pharmaceut Co Ltd | Pharmaceutical preparation for peroral administration of insulinlike growth factor 1 |
-
2013
- 2013-08-23 CN CN201310371772.1A patent/CN103478707B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6306432B1 (en) * | 1997-09-08 | 2001-10-23 | Chiron Corporation | High and low load formulations of IGF-I in multivesicular liposomes |
| CN100350917C (en) * | 1998-04-30 | 2007-11-28 | 纽米库研究澳大利亚控股有限公司 | Food composition and method of using same |
| CN101773194A (en) * | 2010-01-15 | 2010-07-14 | 北京华达杰瑞生物技术有限公司 | Method for extracting soy peptide from soybean meals |
| CN101768618A (en) * | 2010-04-01 | 2010-07-07 | 哈尔滨商业大学 | Method for preparing exorphins from soybean protein isolate by fractional hydrolysis |
Non-Patent Citations (1)
| Title |
|---|
| 刘静,等.多酶复合水解微波加热制备小分子大豆肽.《化学研究与应用》.2007,第19卷(第7期),752-755页. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103478707A (en) | 2014-01-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101143010B (en) | Special-purpose nutritive food for diabetes patient and its preparation method | |
| CN101117352B (en) | Method for producing high temperature stable slow-slaking amidon and uses thereof | |
| CN103815282B (en) | Preparation method for sea cucumber wheaten food | |
| CN104855845A (en) | Natto nutritional health food | |
| CN103960593B (en) | A kind of wheat bran dilated food and preparation method thereof | |
| CN108783437A (en) | A kind of the inflammatory bowel disease full nutrition formula food and its preparation process of predigestion | |
| CN101856099A (en) | Nutritious rice powder and preparation method thereof | |
| CN102657252B (en) | Selenium-enriched pumpkin bread and production method thereof | |
| CN109122870A (en) | Quinquagenarian formula milk powder and preparation method thereof containing maca and ox bone marrow polypeptide | |
| CN109090443A (en) | It is a kind of to replenish the calcium the nutrition formula product of strong bone function with lock calcium | |
| CN103734581B (en) | Yak milk polypeptide nutrient powder being applicable to postoperative crowd's rehabilitation and preparation method thereof | |
| CN104605346B (en) | Flour and preparation method thereof | |
| CN101856369A (en) | Stachyose tablets for calcium supplementation and intestinal micro-ecological regulation | |
| CN103478707B (en) | Insulin-like growth factor-I sustained release tablet and preparation method thereof | |
| CN105707681A (en) | Supermicro black fungus nutrient porridge with blood sugar reducing function and making method thereof | |
| CN103609748A (en) | Hypoglycaemic milk powder for pregnant women and preparation method thereof | |
| CN109924510A (en) | A kind of specific nutrition formula food of suitable tumour patient and preparation method thereof | |
| CN108576539A (en) | Blood pressure lowering vegetable protein solid beverage and preparation method thereof | |
| CN107874245A (en) | Lactose intolerance can be avoided and the nutrient powder of nutrition is provided for lactose intolerance crowd | |
| CN102511882A (en) | Red jujube thick syrup beneficial for improving growth and development | |
| CN103478732B (en) | A kind of matrix sustained-release vitamin functional food and preparation method thereof | |
| CN102424792A (en) | Rana chensinensis puerperium care wine and preparation method thereof | |
| CN101856095A (en) | Method for preparing millet powder for bread and cake | |
| CN107836710B (en) | High-absorptivity iron-supplementing peptide-rich food base material and preparation method and application thereof | |
| CN107242426B (en) | One kind controls sugared solid beverage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160914 Address after: 344000 No. 2111, Linchuan Avenue, Fuzhou, Jiangxi Patentee after: JIANGXI YUJUN BIO-ENGINEERING CO.,LTD. Address before: 2111 No. 334000 Jiangxi city of Fuzhou province Linchuan Avenue (Tsinghua Jinyuan side) Patentee before: Lou Xiuyu |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150408 Termination date: 20210823 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |