CN103478409A - Protein feed produced through peltate yam rhizomes and production method thereof - Google Patents
Protein feed produced through peltate yam rhizomes and production method thereof Download PDFInfo
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Abstract
The invention discloses a production method for protein feed through peltate yam rhizomes. The method comprises the first step of preprocessing of the peltate yam rhizomes, the second step of composite enzymatic hydrolysis, the third step of solid-liquid separation, and the fourth step of production of the protein feed. Starch, cellulose and hemicellulose in the peltate yam rhizomes are hydrolyzed into glucose and xylose through the composite enzymatic hydrolysis method so that dioscin and saponin can be released more easily, and added compound enzyme can serve as a nitrogen source for subsequent yeast cultivation. The water-soluble dioscin and liquid glucose are separated through nanofiltration, and the suppression function on the subsequent production of the protein feed through fermentation of yeast by the dioscin is eliminated. The dioscin is converted into saponin through fermentation of lactobacillus acidophilus, peltate yam rhizome residues containing lactobacillus acidophilus protein are obtained at the same time, yeast protein is obtained through the liquid glucose in yeast fermentation nanofiltration permeate liquid and mixed with peltate yam rhizome fibrous residues, and the protein feed is obtained. Diosgenin saponin is extracted, comprehensive utilization of starch and cellulose is achieved, and exhaust amount and pollution are reduced.
Description
Technical field
The present invention relates to a kind of protein feed and production method thereof, be specially a kind of protein feed and production method thereof of utilizing yellow ginger to produce.
Background technology
Yellow ginger formal name used at school dioscorea zingiberensis wright (Dioscorea zingiberensis.C.H.Wright), have another name called the duration and degree of heating root, with root-like stock, is used as medicine, and is China's tradition parts of generic medicinal plants, is a kind of resources of medicinal plant that potentiality to be exploited is arranged very much, and the title of " medicinal gold " is arranged.Contain abundant Dioscin in the yellow ginger root-like stock, its hydrolysate is that diosgenin is turmeric saponin, and the plant that saponin content is higher in the world is few, mainly is distributed in China and Mexico.At present, the saponin export volume of China is only second to Mexico, occupies the second in the world.
At present, yellow ginger is mainly used to produce saponin, and domestic many manufacturer production turmeric saponins all adopt pre-fermentation acid-hydrolysis method, raw material through soaking fermentation acid hydrolysis, washing, drying, with benzinum extract, condensing crystallizing, last to the product saponin.But in the production process of extracting saponin, can produce the byproducts such as a large amount of Dioscorea. zingiberensis Wright Starchs, cellulose, pigment, cause a large amount of blowdowns, also cause the waste of yellow ginger resource.
Summary of the invention
The object of the present invention is to provide a kind of protein feed and production method thereof of utilizing yellow ginger to produce, can realize Dioscorea. zingiberensis Wright Starch and cellulosic effective utilization, improve the yellow ginger utilization rate, the reducing polluted emission amount.
The present invention is achieved through the following technical solutions:
A kind of method of utilizing yellow ginger to produce protein feed, comprise the steps,
1) yellow ginger pretreatment: be crushed to the 80-100 order after yellow ginger is cleaned, then obtain the slurry that solid concentration is 7.5-12% after adding water to regulate;
2) complex enzyme hydrolysis: the pH value of slurry is adjusted to 6.2-6.5, and adds AMS and calcium chloride after being warming up to 70-80 ℃, insulation enzymolysis 1-2h; Then cool the temperature to 55-60 ℃, the pH value is adjusted to 4.8-5.5, and add carbohydrase, Pullulanase, cellulase, zytase, pectase and 1,4 beta-glucanase, insulation enzymolysis 3-4h, obtain the complex enzyme hydrolysis slurry;
3) Separation of Solid and Liquid: filter complex enzyme hydrolysis liquid and obtain filter residue and filtrate, the NF membrane that is 700 with molecular cut off by filtrate is carried out the film separation, and nanofiltration is retained after liquid and filter residue mix and adds sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and obtain nutrient solution; The pH value of nutrient solution is adjusted to 5.5-6.0, and the access lactobacillus acidophilus is cultivated 60-72h at 34-39 ℃, and Dioscin is converted into to saponin; Then add absolute ethyl alcohol to extract saponin twice, merge ethanol extract, condensing crystallizing obtains saponin; The yellow ginger slag containing the lactobacillus acidophilus mycoprotein that alcohol extract is remaining is standby;
4) produce protein feed: in nanofiltration, see through in liquid and add respectively (NH
4)
2sO
4, urea, dusty yeast, MgSO
4, K
2hPO
4and KH
2pO
4obtain seeing through mixed liquor, see through in mixed liquor and access respectively 4-6ml candida utili and 5-8ml Pachysolen tannophilus at every 100ml in proportion, constant-temperature shaking culture fermentation 48-60h; Add again in step 3), obtain containing fully mixing after the yellow ginger slag of lactobacillus acidophilus mycoprotein, obtain the protein feed containing lactobacillus acidophilus mycoprotein and Yeast protein after low temperature drying.
Preferably, described yellow ginger is the cadmium yellow ginger that water content is 65-70%.
Further, in step 1), the 2-3 that the amount that adds water is the yellow ginger volume doubly.
Preferably, step 2) in, after the AMS and calcium chloride added in slurry, the vigor of corresponding every gram yellow ginger AMS is 70-80U, corresponding every kilogram of yellow ginger calcium ion content is 200-240mg.
Further, step 2) in, corresponding every gram yellow ginger in slurry, the vigor that the vigor that the vigor that the vigor that the vigor that the vigor of carbohydrase is 80-100U, Pullulanase is 5-10U, cellulase is 85-100U, zytase is 30-40U, pectase is 120-150U and 1,4 beta-glucanase is 70-80U.
Preferably, in step 3), filter and adopt the filter press press filtration to realize.
Further, in step 3), in the mixture of every liter of nanofiltration reservation liquid and filter residue, the sodium acetate of interpolation 5-6g, the diammonium hydrogen citrate of 1.5-2.5g, the Tween 80 of 0.8-1.2ml, the magnesium sulfate of 0.8-1.2g, the manganese sulfate of 0.4-0.8g, the dipotassium hydrogen phosphate of 2.5-3.5g obtain nutrient solution in proportion.
Preferably, in step 4), nanofiltration sees through in liquid and adds respectively (the NH that concentration is 20-25g/L
4)
2sO
4, the urea of 6-8g/L, the dusty yeast of 5-6g/L, the MgSO of 0.8-1.2g/L
4, 0.8-1.0g/L K
2hPO
4, 0.8-1.0g/L KH
2pO
4.
Further, in step 4), adopt the shaking table under the speed conditions of the temperature of 27-33 ℃ and 160-200r/min to realize constant-temperature shaking culture.
A kind of protein feed that utilizes yellow ginger to produce, made by above any one described method of utilizing yellow ginger to produce protein feed.
Compared with prior art, the present invention has following useful technique effect:
A kind of method of utilizing yellow ginger to produce protein feed of the present invention, adopt complex enzyme hydrolysis, starch in yellow ginger, cellulose and hydrolysis of hemicellulose are become to glucose and xylose, Dioscin and saponin are more easily discharged, and the complex enzyme added can be used as the nitrogenous source of follow-up microorganism fermented and cultured, by nanofiltration, water-soluble Dioscin and liquid glucose are realized separating again, eliminated Dioscin and follow-up culture propagation has been produced to the inhibitory action of protein feed; Ferment Dioscin is converted into to saponin by lactobacillus acidophilus, obtained the yellow ginger slag containing the lactobacillus acidophilus mycoprotein simultaneously, and the liquid glucose seen through in liquid by the culture propagation nanofiltration obtains Yeast protein, through the protein feed that is mixed to get with the yellow ginger slag, not only realized the extraction of saponin in the yellow ginger, and realized the comprehensive utilization of starch and cellulose accessory substance having reduced discharge capacity and pollution.
Further, the control by the vigor to adding enzyme in step and to the control of other admixture quality, and the control of technological parameter, improve the content of active principle in protein feed, guaranteed the quality of product.
A kind of protein feed that utilizes yellow ginger to produce of the present invention, be rich in Yeast protein, lactobacillus acidophilus mycoprotein, is beneficial to absorbing of letting animals feed, and the surcharge that has improved product is high, and productivity effect is good.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Example 1
A kind of method of utilizing yellow ginger to produce protein feed, comprise the steps,
1) yellow ginger pretreatment: be crushed to 100 orders after yellow ginger is cleaned, then obtain the slurry that solid concentration is 11% after adding water to regulate; Preferred yellow ginger is the cadmium yellow ginger that water content is 70%.Concrete, the amount that adds water is 2 times of yellow ginger volume.
2) complex enzyme hydrolysis: the pH value of slurry is adjusted to 6.2, and adds AMS and calcium chloride after being warming up to 80 ℃, insulation enzymolysis 2h; Then cool the temperature to 60 ℃, the pH value is adjusted to 5.0, and add carbohydrase, Pullulanase, cellulase, zytase, pectase and 1,4 beta-glucanase, insulation enzymolysis 4h, obtain the complex enzyme hydrolysis slurry; Concrete, after the AMS and calcium chloride added in slurry, the vigor of corresponding every gram yellow ginger AMS is 80U, corresponding every kilogram of yellow ginger calcium ion content is 240mg; Corresponding every gram yellow ginger in slurry, the vigor that the vigor that the vigor that the vigor that the vigor that the vigor of carbohydrase is 100U, Pullulanase is 10U, cellulase is 100U, zytase is 40U, pectase is 150U and 1,4 beta-glucanase is 80U.
3) Separation of Solid and Liquid: filter complex enzyme hydrolysis liquid and obtain filter residue and filtrate, the NF membrane that is 700 with molecular cut off by filtrate is carried out the film separation, and nanofiltration is retained after liquid and filter residue mix and adds sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and obtain nutrient solution; The pH value of nutrient solution is adjusted to 6.0, and the access lactobacillus acidophilus is cultivated 72h at 37 ℃, and Dioscin is converted into to saponin; Then add absolute ethyl alcohol to extract saponin twice, merge ethanol extract, condensing crystallizing obtains saponin; The yellow ginger slag containing the lactobacillus acidophilus mycoprotein that alcohol extract is remaining is standby; Concrete, filter and adopt the filter press press filtration to realize, in the mixture of every liter of nanofiltration reservation liquid and filter residue, the sodium acetate of interpolation 5g, the diammonium hydrogen citrate of 1.5g, the Tween 80 of 0.8ml, the magnesium sulfate of 0.8g, the manganese sulfate of 0.4g, the dipotassium hydrogen phosphate of 2.5g obtain nutrient solution in proportion.
4) produce protein feed: in nanofiltration, see through in liquid and add respectively (NH
4)
2sO
4, urea, dusty yeast, MgSO
4, K
2hPO
4and KH
2pO
4obtain seeing through mixed liquor, see through in mixed liquor and access respectively 6ml candida utili and 5ml Pachysolen tannophilus at every 100ml in proportion, constant-temperature shaking culture fermentation 60h; Add again in step 3), obtain containing fully mixing after the yellow ginger slag of lactobacillus acidophilus mycoprotein, obtain the protein feed containing lactobacillus acidophilus mycoprotein and Yeast protein after low temperature drying.Concrete, nanofiltration sees through in liquid and adds respectively (the NH that concentration is 22g/L
4)
2sO
4, the urea of 7.5g/L, the dusty yeast of 6g/L, the MgSO of 1.0g/L
4, 1.0g/L K
2hPO
4, 0.9g/L KH
2pO
4; The shaking table of employing under the speed conditions of the temperature of 30 ℃ and 180r/min realized constant-temperature shaking culture.
Example 2
A kind of method of utilizing yellow ginger to produce protein feed, comprise the steps,
1) yellow ginger pretreatment: be crushed to 100 orders after yellow ginger is cleaned, then obtain the slurry that solid concentration is 10% after adding water to regulate; Preferred yellow ginger is the cadmium yellow ginger that water content is 65%.Concrete, the amount that adds water is 2 times of yellow ginger volume.
2) complex enzyme hydrolysis: the pH value of slurry is adjusted to 6.5, and adds AMS and calcium chloride after being warming up to 75 ℃, insulation enzymolysis 1h; Then cool the temperature to 58 ℃, the pH value is adjusted to 5.3, and add carbohydrase, Pullulanase, cellulase, zytase, pectase and 1,4 beta-glucanase, insulation enzymolysis 3h, obtain the complex enzyme hydrolysis slurry; Concrete, after the AMS and calcium chloride added in slurry, the vigor of corresponding every gram yellow ginger AMS is 75U, corresponding every kilogram of yellow ginger calcium ion content is 220mg; Corresponding every gram yellow ginger in slurry, the vigor that the vigor that the vigor that the vigor that the vigor that the vigor of carbohydrase is 80U, Pullulanase is 8U, cellulase is 85U, zytase is 30U, pectase is 130U and 1,4 beta-glucanase is 75U.
3) Separation of Solid and Liquid: filter complex enzyme hydrolysis liquid and obtain filter residue and filtrate, the NF membrane that is 700 with molecular cut off by filtrate is carried out the film separation, and nanofiltration is retained after liquid and filter residue mix and adds sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and obtain nutrient solution; The pH value of nutrient solution is adjusted to 5.5, and the access lactobacillus acidophilus is cultivated 66h at 37 ℃, and Dioscin is converted into to saponin; Then add absolute ethyl alcohol to extract saponin twice, merge ethanol extract, condensing crystallizing obtains saponin; The yellow ginger slag containing the lactobacillus acidophilus mycoprotein that alcohol extract is remaining is standby; Concrete, filter and adopt the filter press press filtration to realize, in the mixture of every liter of nanofiltration reservation liquid and filter residue, the sodium acetate of interpolation 6g, the diammonium hydrogen citrate of 2.5g, the Tween 80 of 1.2ml, the magnesium sulfate of 1.2g, the manganese sulfate of 0.8g, the dipotassium hydrogen phosphate of 3.5g obtain nutrient solution in proportion.
4) produce protein feed: in nanofiltration, see through in liquid and add respectively (NH
4)
2sO
4, urea, dusty yeast, MgSO
4, K
2hPO
4and KH
2pO
4obtain seeing through mixed liquor, see through in mixed liquor and access respectively 6ml candida utili and 7ml Pachysolen tannophilus at every 100ml in proportion, constant-temperature shaking culture fermentation 60h; Add again in step 3), obtain containing fully mixing after the yellow ginger slag of lactobacillus acidophilus mycoprotein, obtain the protein feed containing lactobacillus acidophilus mycoprotein and Yeast protein after low temperature drying.Concrete, nanofiltration sees through in liquid and adds respectively (the NH that concentration is 23g/L
4)
2sO
4, the urea of 7g/L, the dusty yeast of 5.5g/L, the MgSO of 1.1g/L
4, 0.9g/L K
2hPO
4, 1.0g/L KH
2pO
4.The shaking table of employing under the speed conditions of the temperature of 30 ℃ and 160r/min realized constant-temperature shaking culture.
Example 3
A kind of method of utilizing yellow ginger to produce protein feed, comprise the steps,
1) yellow ginger pretreatment: be crushed to 80 orders after yellow ginger is cleaned, then obtain the slurry that solid concentration is 7.5% after adding water to regulate; Preferred yellow ginger is the cadmium yellow ginger that water content is 65%.Concrete, the amount that adds water is 3 times of yellow ginger volume.
2) complex enzyme hydrolysis: the pH value of slurry is adjusted to 6.3, and adds AMS and calcium chloride after being warming up to 70 ℃, insulation enzymolysis 2h; Then cool the temperature to 55 ℃, the pH value is adjusted to 4.8, and add carbohydrase, Pullulanase, cellulase, zytase, pectase and 1,4 beta-glucanase, insulation enzymolysis 3h, obtain the complex enzyme hydrolysis slurry; Concrete, after the AMS and calcium chloride added in slurry, the vigor of corresponding every gram yellow ginger AMS is 70U, corresponding every kilogram of yellow ginger calcium ion content is 200mg; Corresponding every gram yellow ginger in slurry, the vigor that the vigor that the vigor that the vigor that the vigor that the vigor of carbohydrase is 90U, Pullulanase is 5U, cellulase is 90U, zytase is 35U, pectase is 120U and 1,4 beta-glucanase is 5.6U.
3) Separation of Solid and Liquid: filter complex enzyme hydrolysis liquid and obtain filter residue and filtrate, the NF membrane that is 700 with molecular cut off by filtrate is carried out the film separation, and nanofiltration is retained after liquid and filter residue mix and adds sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and obtain nutrient solution; The pH value of nutrient solution is adjusted to 5.6, and the access lactobacillus acidophilus is cultivated 60h at 34 ℃, and Dioscin is converted into to saponin; Then add absolute ethyl alcohol to extract saponin twice, merge ethanol extract, condensing crystallizing obtains saponin; The yellow ginger slag containing the lactobacillus acidophilus mycoprotein that alcohol extract is remaining is standby; Concrete, filter and adopt the filter press press filtration to realize, in the mixture of every liter of nanofiltration reservation liquid and filter residue, the sodium acetate of interpolation 5g, the diammonium hydrogen citrate of 2.0g, the Tween 80 of 0.9ml, the magnesium sulfate of 1.0g, the manganese sulfate of 0.5g, the dipotassium hydrogen phosphate of 2.5g obtain nutrient solution in proportion.
4) produce protein feed: in nanofiltration, see through in liquid and add respectively (NH
4)
2sO
4, urea, dusty yeast, MgSO
4, K
2hPO
4and KH
2pO
4obtain seeing through mixed liquor, see through in mixed liquor and access respectively 4ml candida utili and 5ml Pachysolen tannophilus at every 100ml in proportion, constant-temperature shaking culture fermentation 48h; Add again in step 3), obtain containing fully mixing after the yellow ginger slag of lactobacillus acidophilus mycoprotein, obtain the protein feed containing lactobacillus acidophilus mycoprotein and Yeast protein after low temperature drying.Concrete, nanofiltration sees through in liquid and adds respectively (the NH that concentration is 20g/L
4)
2sO
4, the urea of 6g/L, the dusty yeast of 5g/L, the MgSO of 0.8g/L
4, 0.8g/L K
2hPO
4, 0.8g/L KH
2pO
4.The shaking table of employing under the speed conditions of the temperature of 27 ℃ and 200r/min realized constant-temperature shaking culture.
Example 4
A kind of method of utilizing yellow ginger to produce protein feed, comprise the steps,
1) yellow ginger pretreatment: be crushed to 90 orders after yellow ginger is cleaned, then obtain the slurry that solid concentration is 12% after adding water to regulate; Preferred yellow ginger is the cadmium yellow ginger that water content is 68%.Concrete, the amount that adds water is 3 times of yellow ginger volume.
2) complex enzyme hydrolysis: the pH value of slurry is adjusted to 6.4, and adds AMS and calcium chloride after being warming up to 72 ℃, insulation enzymolysis 1h; Then cool the temperature to 56 ℃, the pH value is adjusted to 5.5, and add carbohydrase, Pullulanase, cellulase, zytase, pectase and 1,4 beta-glucanase, insulation enzymolysis 4h, obtain the complex enzyme hydrolysis slurry; Concrete, after the AMS and calcium chloride added in slurry, the vigor of corresponding every gram yellow ginger AMS is 72U, corresponding every kilogram of yellow ginger calcium ion content is 210mg; Corresponding every gram yellow ginger in slurry, the vigor that the vigor that the vigor that the vigor that the vigor that the vigor of carbohydrase is 85U, Pullulanase is 6U, cellulase is 85U, zytase is 30U, pectase is 140U and 1,4 beta-glucanase is 75U.
3) Separation of Solid and Liquid: filter complex enzyme hydrolysis liquid and obtain filter residue and filtrate, the NF membrane that is 700 with molecular cut off by filtrate is carried out the film separation, and nanofiltration is retained after liquid and filter residue mix and adds sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and obtain nutrient solution; The pH value of nutrient solution is adjusted to 5.7, and the access lactobacillus acidophilus is cultivated 70h at 39 ℃, and Dioscin is converted into to saponin; Then add absolute ethyl alcohol to extract saponin twice, merge ethanol extract, condensing crystallizing obtains saponin; The yellow ginger slag containing the lactobacillus acidophilus mycoprotein that alcohol extract is remaining is standby; Concrete, filter and adopt the filter press press filtration to realize, in the mixture of every liter of nanofiltration reservation liquid and filter residue, the sodium acetate of interpolation 6g, the diammonium hydrogen citrate of 1.7g, the Tween 80 of 1.0ml, the magnesium sulfate of 1.1g, the manganese sulfate of 0.6g, the dipotassium hydrogen phosphate of 3.5g obtain nutrient solution in proportion.
4) produce protein feed: in nanofiltration, see through in liquid and add respectively (NH
4)
2sO
4, urea, dusty yeast, MgSO
4, K
2hPO
4and KH
2pO
4obtain seeing through mixed liquor, see through in mixed liquor and access respectively 5ml candida utili and 6ml Pachysolen tannophilus at every 100ml in proportion, constant-temperature shaking culture fermentation 55h; Add again in step 3), obtain containing fully mixing after the yellow ginger slag of lactobacillus acidophilus mycoprotein, obtain the protein feed containing lactobacillus acidophilus mycoprotein and Yeast protein after low temperature drying.Concrete, nanofiltration sees through in liquid and adds respectively (the NH that concentration is 21g/L
4)
2sO
4, the urea of 7g/L, the dusty yeast of 6g/L, the MgSO of 0.9g/L
4, 0.9g/L K
2hPO
4, 0.9g/L KH
2pO
4.The shaking table of employing under the speed conditions of the temperature of 33 ℃ and 170r/min realized constant-temperature shaking culture.
Example 5
A kind of method of utilizing yellow ginger to produce protein feed, comprise the steps,
1) yellow ginger pretreatment: be crushed to 80 orders after yellow ginger is cleaned, then obtain the slurry that solid concentration is 9% after adding water to regulate; Preferred yellow ginger is the cadmium yellow ginger that water content is 66%.Concrete, the amount that adds water is 3 times of yellow ginger volume.
2) complex enzyme hydrolysis: the pH value of slurry is adjusted to 6.5, and adds AMS and calcium chloride after being warming up to 78 ℃, insulation enzymolysis 2h; Then cool the temperature to 57 ℃, the pH value is adjusted to 4.9, and add carbohydrase, Pullulanase, cellulase, zytase, pectase and 1,4 beta-glucanase, insulation enzymolysis 3h, obtain the complex enzyme hydrolysis slurry; Concrete, after the AMS and calcium chloride added in slurry, the vigor of corresponding every gram yellow ginger AMS is 77U, corresponding every kilogram of yellow ginger calcium ion content is 230mg; Corresponding every gram yellow ginger in slurry, the vigor that the vigor that the vigor that the vigor that the vigor that the vigor of carbohydrase is 95U, Pullulanase is 7U, cellulase is 100U, zytase is 40U, pectase is 150U and 1,4 beta-glucanase is 80U.
3) Separation of Solid and Liquid: filter complex enzyme hydrolysis liquid and obtain filter residue and filtrate, the NF membrane that is 700 with molecular cut off by filtrate is carried out the film separation, and nanofiltration is retained after liquid and filter residue mix and adds sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and obtain nutrient solution; The pH value of nutrient solution is adjusted to 5.8, and the access lactobacillus acidophilus is cultivated 75h at 35 ℃, and Dioscin is converted into to saponin; Then add absolute ethyl alcohol to extract saponin twice, merge ethanol extract, condensing crystallizing obtains saponin; The yellow ginger slag containing the lactobacillus acidophilus mycoprotein that alcohol extract is remaining is standby; Concrete, filter and adopt the filter press press filtration to realize, in the mixture of every liter of nanofiltration reservation liquid and filter residue, the sodium acetate of interpolation 5g, the diammonium hydrogen citrate of 2.3g, the Tween 80 of 1.1ml, the magnesium sulfate of 0.9g, the manganese sulfate of 0.7g, the dipotassium hydrogen phosphate of 2.5g obtain nutrient solution in proportion.
4) produce protein feed: in nanofiltration, see through in liquid and add respectively (NH
4)
2sO
4, urea, dusty yeast, MgSO
4, K
2hPO
4and KH
2pO
4obtain seeing through mixed liquor, see through in mixed liquor and access respectively 6ml candida utili and 7ml Pachysolen tannophilus at every 100ml in proportion, constant-temperature shaking culture fermentation 50h; Add again in step 3), obtain containing fully mixing after the yellow ginger slag of lactobacillus acidophilus mycoprotein, obtain the protein feed containing lactobacillus acidophilus mycoprotein and Yeast protein after low temperature drying.Concrete, nanofiltration sees through in liquid and adds respectively (the NH that concentration is 24g/L
4)
2sO
4, the urea of 8g/L, the dusty yeast of 5.5g/L, the MgSO of 1.2g/L
4, 1.0g/L K
2hPO
4, 1.0g/L KH
2pO
4.The shaking table of employing under the speed conditions of the temperature of 31 ℃ and 190r/min realized constant-temperature shaking culture.
Claims (10)
1. a method of utilizing yellow ginger to produce protein feed, is characterized in that, comprise the steps,
1) yellow ginger pretreatment: be crushed to the 80-100 order after yellow ginger is cleaned, then obtain the slurry that solid concentration is 7.5-12% after adding water to regulate;
2) complex enzyme hydrolysis: the pH value of slurry is adjusted to 6.2-6.5, and adds AMS and calcium chloride after being warming up to 70-80 ℃, insulation enzymolysis 1-2h; Then cool the temperature to 55-60 ℃, the pH value is adjusted to 4.8-5.5, and add carbohydrase, Pullulanase, cellulase, zytase, pectase and 1,4 beta-glucanase, insulation enzymolysis 3-4h, obtain the complex enzyme hydrolysis slurry;
3) Separation of Solid and Liquid: filter complex enzyme hydrolysis liquid and obtain filter residue and filtrate, the NF membrane that is 700 with molecular cut off by filtrate is carried out the film separation, and nanofiltration is retained after liquid and filter residue mix and adds sodium acetate, diammonium hydrogen citrate, Tween 80, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and obtain nutrient solution; The pH value of nutrient solution is adjusted to 5.5-6.0, and the access lactobacillus acidophilus is cultivated 60-72h at 34-39 ℃, and Dioscin is converted into to saponin; Then add absolute ethyl alcohol to extract saponin twice, merge ethanol extract, condensing crystallizing obtains saponin; The yellow ginger slag containing the lactobacillus acidophilus mycoprotein that alcohol extract is remaining is standby;
4) produce protein feed: in nanofiltration, see through in liquid and add respectively (NH
4)
2sO
4, urea, dusty yeast, MgSO
4, K
2hPO
4and KH
2pO
4obtain seeing through mixed liquor, see through in mixed liquor and access respectively 4-6ml candida utili and 5-8ml Pachysolen tannophilus at every 100ml in proportion, constant-temperature shaking culture fermentation 48-60h; Add again in step 3), obtain containing fully mixing after the yellow ginger slag of lactobacillus acidophilus mycoprotein, obtain the protein feed containing lactobacillus acidophilus mycoprotein and Yeast protein after low temperature drying.
2. a kind of method of utilizing yellow ginger to produce protein feed according to claim 1, is characterized in that, described yellow ginger is the cadmium yellow ginger that water content is 65-70%.
3. a kind of method of utilizing yellow ginger to produce protein feed according to claim 1 and 2, is characterized in that, in step 1), the 2-3 that the amount that adds water is the yellow ginger volume doubly.
4. a kind of method of utilizing yellow ginger to produce protein feed according to claim 1, it is characterized in that step 2) in, after the AMS and calcium chloride added in slurry, the vigor of corresponding every gram yellow ginger AMS is 70-80U, and corresponding every kilogram of yellow ginger calcium ion content is 200-240mg.
5. according to a kind of described method of utilizing yellow ginger to produce protein feed of claim 1 or 4, it is characterized in that, step 2) in, corresponding every gram yellow ginger in slurry, the vigor that the vigor that the vigor that the vigor that the vigor that the vigor of carbohydrase is 80-100U, Pullulanase is 5-10U, cellulase is 85-100U, zytase is 30-40U, pectase is 120-150U and 1,4 beta-glucanase is 70-80U.
6. a kind of method of utilizing yellow ginger to produce protein feed according to claim 1, is characterized in that, in step 3), filters and adopt the filter press press filtration to realize.
7. according to a kind of described method of utilizing yellow ginger to produce protein feed of claim 1 or 6, it is characterized in that, in step 3), in the mixture of every liter of nanofiltration reservation liquid and filter residue, the sodium acetate of interpolation 5-6g, the diammonium hydrogen citrate of 1.5-2.5g, the Tween 80 of 0.8-1.2ml, the magnesium sulfate of 0.8-1.2g, the manganese sulfate of 0.4-0.8g, the dipotassium hydrogen phosphate of 2.5-3.5g obtain nutrient solution in proportion.
8. a kind of method of utilizing yellow ginger to produce protein feed according to claim 1, is characterized in that, in step 4), nanofiltration sees through in liquid and adds respectively (the NH that concentration is 20-25g/L
4)
2sO
4, the urea of 6-8g/L, the dusty yeast of 5-6g/L, the MgSO of 0.8-1.2g/L
4, 0.8-1.0g/L K
2hPO
4, 0.8-1.0g/L KH
2pO
4.
9. according to a kind of described method of utilizing yellow ginger to produce protein feed of claim 1 or 8, it is characterized in that, in step 4), adopt the shaking table under the speed conditions of the temperature of 27-33 ℃ and 160-200r/min to realize constant-temperature shaking culture.
10. a protein feed that utilizes yellow ginger to produce, is characterized in that, in claim 1-9, the described method of utilizing yellow ginger to produce protein feed of any one makes.
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