CN103472060B - A kind of urine calcium ion Test paper and preparation method thereof - Google Patents
A kind of urine calcium ion Test paper and preparation method thereof Download PDFInfo
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- CN103472060B CN103472060B CN201310471065.XA CN201310471065A CN103472060B CN 103472060 B CN103472060 B CN 103472060B CN 201310471065 A CN201310471065 A CN 201310471065A CN 103472060 B CN103472060 B CN 103472060B
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- urine calcium
- ion test
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Abstract
The invention discloses a kind of urine calcium ion Test paper and preparation method thereof, this Test paper comprises substrate and filter paper, described substrate is fixedly connected with described filter paper, described filter paper takes out after filter paper fully soaks in A liquid, obtained after dry, described A liquid contains: the aqueous solution of 1wt% ~ 1.5wt% damping fluid, 0.35wt% ~ 0.58wt% OCPC, 10wt% ~ 12.5wt% surfactant, 0.35wt% ~ 0.55wt% screening agent, 0.03wt% ~ 0.05wt% lemon yellow.This urine calcium ion Test paper prepared by this case, in the basic conditions, calcium ion concentration is within the scope of 0 ~ 10mmol/L, present from pale yellow to wine-colored obvious color range, be easy to visual resolution, method for preparing test paper is simple, cost of manufacture is cheap, easy to operate, stable performance, measurement result quick and precisely.
Description
Technical field
The present invention relates to urine detection field, particularly a kind of urine calcium ion Test paper and preparation method thereof.
Background technology
Calcium is one of element of needed by human, is also one of element that people's in-vivo content is maximum, closely related with various physiological activity.The change of urine calcium can reflect the change of blood calcium, and the exception (increase or reduce) of urine calcium is relevant to various diseases, as relevant in hypercalcinemia, hyperparathyroidism, Huppert's disease, bone malignant tumour etc.Therefore, urinate calcium catalyst to have great importance.
The conventional assay method of current calcium has compleximetry, atomic absorption method, these methods all need the instrument and equipment of being correlated with and meet specially trained testing staff, complicated operation, and detection speed is slow, and these special instrument equipment are not easy to carry, testing cost is expensive.
Summary of the invention
For the deficiencies in the prior art part, the invention provides a kind of urine calcium ion Test paper and preparation method thereof, this preparation method is simple and efficient, and cost of manufacture is cheap, and this test paper is easy to use, and testing result quick and precisely.
Technical scheme of the present invention is summarized as follows:
A kind of urine calcium ion Test paper, comprises substrate and filter paper, and on the substrate, immerse in described filter paper and have A liquid, described A liquid comprises in described filter paper attachment:
The aqueous solution of 1wt% ~ 1.5wt% damping fluid, 0.35wt% ~ 0.58wt% OCPC, 10wt% ~ 12.5wt% surfactant, 0.35wt% ~ 0.55wt% screening agent and 0.03wt% ~ 0.05wt% lemon yellow.
Preferably, described a kind of urine calcium ion Test paper, described damping fluid is selected from 2-amino-2-methyl-1-propanol, AMPD, N-cyclohexyl-Homotaurine, N-methyl-D-glucosamine or its combination; Described surfactant is selected from polyvinylpyrrolidone, Pluronic F-127 lauric acid ether, polyglycol or its combination; Described screening agent is selected from oxine, HQS or its combination.
Preferably, described a kind of urine calcium ion Test paper, pH=10 ~ 12 of described A liquid.
Preferably, described a kind of urine calcium ion Test paper, described substrate material is Corvic.
Preferably, described a kind of urine calcium ion Test paper, described filter paper is common quantitative filter paper or common qualitative filter paper.
A preparation method for urine calcium ion Test paper, comprises the steps:
(1) A liquid is configured: 10 ~ 15g damping fluid, 3.5 ~ 5.8g OCPC, 100 ~ 125g surfactant, 3.5 ~ 5.5g screening agent, 0.3 ~ 0.5g lemon yellow are put into container, adds water to 1000mL;
Wherein, described damping fluid is selected from 2-amino-2-methyl-1-propanol, AMPD, N-cyclohexyl-Homotaurine, N-methyl-D-glucosamine or its combination; Described surfactant is selected from polyvinylpyrrolidone, Pluronic F-127 lauric acid ether, polyglycol or its combination; Described screening agent is selected from oxine, HQS or its combination;
(2) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 50 ~ 90 DEG C, drying time is 10 ~ 20 minutes;
(3) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine calcium ion Test paper.
Preferably, the preparation method of described a kind of urine calcium ion Test paper, pH=10 ~ 12 of described A liquid.
Preferably, the preparation method of described a kind of urine calcium ion Test paper, described rectangle paper slip is of a size of 0.65 × 0.85cm.
The invention has the beneficial effects as follows: this urine calcium ion Test paper of preparation, in the basic conditions, OCPC can react rapidly with the calcium ion in urine, and calcium ion concentration is within the scope of 0 ~ 10mmol/L, test paper presents from pale yellow to wine-colored obvious color range, be easy to visual resolution, and not by the interference of other ions in urine, method for preparing test paper is simple, cost of manufacture is cheap, easy to operate, stable performance, measurement result quick and precisely.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, can implement according to this with reference to instructions word to make those skilled in the art.
Embodiment 1
The present embodiment provides a kind of urine calcium ion Test paper, and it prepares as follows:
(1) A liquid is configured: 12.5g2-amino-2-methyl-1-propyl alcohol, 4.6g OCPC, 113g polyvinylpyrrolidone, 4.5g8-hydroxyquinoline, 0.4g lemon yellow are put into volumetric flask, adds water to 1000mL; Through the test of pH test paper, the pH=11 of A liquid;
(2) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 70 DEG C, drying time is 15 minutes;
(3) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine calcium ion Test paper.
Embodiment 2
The present embodiment provides a kind of urine calcium ion Test paper, and it can also prepare as follows:
(1) A liquid is configured: by 12g2-amino-2-methyl-1, ammediol, 5.5g OCPC, 95g polyvinylpyrrolidone, 20g Pluronic F-127 lauric acid ether, 4.8g8-hydroxyquinoline, 0.4g lemon yellow put into volumetric flask, add water to 1000mL; Through the test of pH test paper, the pH=11 of A liquid;
(2) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 60 DEG C, drying time is 15 minutes;
(3) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine calcium ion Test paper.
Embodiment 3
The preparation method of standard color comparison card:
(1) the calcium ion standard solution of 0mmol/L, 2.5mmol/L, 5.0mmol/L, 7.5mmol/L, 10mmol/L is configured;
(2) be dipped in respectively in five kinds of above-mentioned calcium ion standard solution by the Test paper that embodiment 1 or embodiment 2 prepare, take out, dry after 2 seconds, within 20 seconds, colour developing is complete afterwards;
(3) prepare colorimetric card according to the colour developing situation of each test paper, the color range of colorimetric card is pale yellow to wine-colored 5 color ranges.
Embodiment 4
Urine calcium ion Test paper using method:
The Test paper that embodiment 1 or 2 prepares is immersed in liquid to be measured, take out after 2 seconds, dry, within 20 seconds, colour developing is complete afterwards, compare with standard color comparison card subsequently, be the concentration of liquid to be measured with the concentration corresponding to the color on the immediate standard color comparison card of color on Test paper, if between two color ranges, then can estimate and read calcium ion concentration.
The pH scope of liquid to be measured should be 10 ~ 12, if the pH value of liquid to be measured is not within the scope of this, can be regulated in advance.
Although embodiment of the present invention are open as above, but it is not restricted to listed in instructions and embodiment utilization, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend described.
Claims (7)
1. a urine calcium ion Test paper, comprises substrate and filter paper, and described filter paper attachment on the substrate, is characterized in that, immersing in described filter paper has A liquid, and described A liquid comprises:
The aqueous solution of 1wt% ~ 1.5wt% damping fluid, 0.35wt% ~ 0.58wt% OCPC, 10wt% ~ 12.5wt% surfactant, 0.35wt% ~ 0.55wt% screening agent and 0.03wt% ~ 0.05wt% lemon yellow;
Described damping fluid is selected from 2-amino-2-methyl-1-propanol, AMPD or its combination; Described surfactant is the combination of polyvinylpyrrolidone and Pluronic F-127 lauric acid ether; Described screening agent is selected from oxine, HQS or its combination.
2. a kind of urine calcium ion Test paper according to claim 1, is characterized in that, pH=10 ~ 12 of described A liquid.
3. a kind of urine calcium ion Test paper according to claim 1, it is characterized in that, described substrate material is Corvic.
4. a kind of urine calcium ion Test paper according to claim 1, is characterized in that, described filter paper is common quantitative filter paper or common qualitative filter paper.
5. a preparation method for urine calcium ion Test paper, its feature comprises the steps:
(1) A liquid is configured: 10 ~ 15g damping fluid, 3.5 ~ 5.8g OCPC, 100 ~ 125g surfactant, 3.5 ~ 5.5g screening agent, 0.3 ~ 0.5g lemon yellow are put into container, adds water to 1000mL;
Wherein, described damping fluid is selected from 2-amino-2-methyl-1-propanol, AMPD or its combination; Described surfactant is the combination of polyvinylpyrrolidone and Pluronic F-127 lauric acid ether; Described screening agent is selected from oxine, HQS or its combination;
(2) immersed by filter paper in A liquid, take out after fully soaking, be placed in the vacuum drying chamber drying that temperature is 50 ~ 90 DEG C, drying time is 10 ~ 20 minutes;
(3) dried filter paper and substrate are fixed, be cut into rectangle paper slip, make a kind of urine calcium ion Test paper.
6. the preparation method of a kind of urine calcium ion Test paper according to claim 5, is characterized in that, pH=10 ~ 12 of described A liquid.
7. the preparation method of a kind of urine calcium ion Test paper according to claim 5, it is characterized in that, described rectangle paper slip is of a size of 0.65 × 0.85cm.
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104764738B (en) * | 2014-01-04 | 2018-04-17 | 无锡市申瑞生物制品有限公司 | A kind of calcium qualitative test bar and preparation method thereof |
| CN103901034B (en) * | 2014-04-29 | 2016-07-06 | 中国科学院苏州生物医学工程技术研究所 | A kind of detectable bar detecting microalbumin in urine and preparation method thereof |
| CN105181689B (en) * | 2015-09-21 | 2018-03-23 | 济南大学 | A kind of preparation method of the Test paper of eriochrome black T urinary calcium ion |
| CN105203536B (en) * | 2015-09-21 | 2018-03-23 | 济南大学 | A kind of preparation method of the Test paper of urinary calcium ion |
| CN106198523A (en) * | 2016-07-06 | 2016-12-07 | 哈尔滨贝贝凯尔科技发展有限公司 | Trace element detection reagent paper and preparation method thereof |
| CN106770254A (en) * | 2017-01-03 | 2017-05-31 | 长春迪瑞医疗科技股份有限公司 | A kind of urine calcium ion Test paper and preparation method thereof |
| CN111141898A (en) * | 2019-12-31 | 2020-05-12 | 东北大学 | Urinary calculus risk factor detection paper chip and preparation method thereof |
| CN111239120A (en) * | 2020-02-26 | 2020-06-05 | 绍兴市人民医院 | Blood calcium ion detection test paper |
| CN111650191A (en) * | 2020-06-12 | 2020-09-11 | 吉林基蛋生物科技有限公司 | Preparation method of anti-magnesium-ion-interference urine calcium ion detection test paper |
| CN112285367B (en) * | 2020-12-24 | 2021-05-25 | 中生北控生物科技股份有限公司 | Kit for detecting calcium ions and application thereof |
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| US4871678A (en) * | 1985-04-24 | 1989-10-03 | Beschrankter Haftung | Agent and process for the determination of calcium |
| CN102759524A (en) * | 2011-04-28 | 2012-10-31 | 爱科来株式会社 | Dry test strip for measuring calcium |
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|---|---|---|---|---|
| JPS5436996A (en) * | 1977-08-29 | 1979-03-19 | Motoshi Kitamura | Calcium analytical method |
| DE10061140A1 (en) * | 2000-12-08 | 2002-06-13 | Merck Patent Gmbh | Methods and means for determining total hardness |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4871678A (en) * | 1985-04-24 | 1989-10-03 | Beschrankter Haftung | Agent and process for the determination of calcium |
| CN102759524A (en) * | 2011-04-28 | 2012-10-31 | 爱科来株式会社 | Dry test strip for measuring calcium |
Non-Patent Citations (1)
| Title |
|---|
| 血清钙邻甲酚酞络合酮测定法;刘文虎;《临床检验杂志》;19840430;第2卷(第4期);全文 * |
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