CN103461377B - Sterilizing and withering agent and method for treating field plants with banana wilt by applying same - Google Patents
Sterilizing and withering agent and method for treating field plants with banana wilt by applying same Download PDFInfo
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Abstract
The invention discloses a sterilizing and withering agent and a method for treating field plants with banana wilt by applying the same. The sterilizing and withering agent disclosed by the invention can comprise the following raw materials in percentage by mass: 0.045%-4.5% of prochloraz, 0.05%-5% of carbendazim, 0.041%-4.1% of glyphosate, 1%-10% of dimethyl sulfoxide, 1%-10% of ethanediol, 0.001%-0.005% of xanthan gum, 1%-10% of dispersant and the balance of water. The sterilizing and withering agent provides a strong technical support for field prevention and control of the banana wilt, is quick in action and low in cost, can be used for effectively controlling disease spread and dissemination, reducing the incidence rate and reducing the economic loss, has remarkable social and economic benefits, and is very suitable for field popularization and application.
Description
Technical field
The present invention relates to a kind of sterilization and cause withered dose and apply the method for its process banana blight land for growing field crops diseased plant.
Background technology
The banana (Musa spp.) of broad sense comprises banana (Banana) and the large class of plantain (Plantain) two, belong to Musaceae (Musaece) Musa (Musa), be second-biggest-in-the-world fruit crop, output is only second to citrus.Banana is the large-scale draft of perennial evergreen, and monocotyledon, biomass is huge.The main breed of China's banana is Musa AAA monoid, and Dwarf Cavendish subgroup, i.e. fragrant tooth any of several broadleaf plants, plant height is generally 2-4 rice.Banana plant is made up of root, bulb, false stem, blade, fruit axle and fruit ear etc.The base portion of plant claims bulb (also known as ball stem), folds the mother culm wrapped up in claim false stem (also known as false stem stalk) by leaf sheath.Bulb is commonly called as banana head, be take root be, the organ of blade, suction bud, be also the centre of supply and storage nutriment.
Banana blight, also known as research of fusarium wilt disesase of banana, Panama disease, yellowtop, it is a kind of destructive disease causing plant death by destroying plant vascular bundle caused by Fusarium oxysporum Cuba specialized form (Fusarium oxyporum f.sp.cubense).At present, except Mediterranean, the Indian Ocean, some island countries of the South Pacific Ocean, all there is the report suffering this disease hazard nearly all banana plantation.In China Guangdong, Guangxi, Fujian, Hainan, Yunnan and Taiwan etc. all there is this disease in main banana plantation, and morbidity to increase the weight of trend obvious.Banana blight pathogenic bacteria, by propagation such as the residual body of diseased plant, farming instrument, the seedling that carries disease germs, soil and flowing water, make lesion constantly expand, and spread fast, control difficulty.Pathogenic bacteria can surely grow and spread, cause vascular bundle to block, progressively make plant leaf yellow after invading host from banana root system in vascular bundle, false basal part of stem cracking, death of finally wilting.There is no desirable banana blight pesticide control at present, the incidence of disease comparatively Gao Jiao garden can only lie waste or replant other crop.Therefore, when there is fragmentary morbidity strain in field, process of should taking measures in time, avoids germ diffusion towards periphery centered by the residual body of diseased plant to spread.
At present the method that banana blight land for growing field crops diseased plant processes is mainly contained: 1. cut down diseased plant and throw aside in field and be exposed to the sun; 2. quicklime is spilt; 3. herbicide spraying carries out disinfection; 4. weed killer herbicide and bactericide destruction plant etc. is injected.With upper type or operate simple and easy but Disinfection Effect is undesirable, or there is certain effect but complicated operation, be unfavorable for field practical application.
Summary of the invention
The object of this invention is to provide a kind of sterilization and cause withered dose and apply the method for its process banana blight land for growing field crops diseased plant.
The invention provides a kind of sterilization and cause withered dose, comprise Prochloraz, carbendazim and glyphosate, the mass percentage of described Prochloraz is that 0.045%-4.5%(is as 0.045%-0.45%, 0.45%-4.5%, 0.045%, 0.45% or 4.5%), the mass percentage of described carbendazim is that 0.05%-5%(is as 0.05%-0.5%, 0.5%-5%, 0.05%, 0.5% or 5%), the mass percentage of described glyphosate is that 0.041%-4.1%(is as 0.041%-0.41%, 0.41%-4.1%, 0.041%, 0.41% or 4.1%).
Described sterilization causes withered dose can by Prochloraz, carbendazim, glyphosate, dimethyl sulfoxide (DMSO), ethylene glycol, xanthans, dispersant and water composition, the mass percentage of described Prochloraz is that 0.045%-4.5%(is as 0.045%-0.45%, 0.45%-4.5%, 0.045%, 0.45% or 4.5%), the mass percentage of described carbendazim is that 0.05%-5%(is as 0.05%-0.5%, 0.5%-5%, 0.05%, 0.5% or 5%), the mass percentage of described glyphosate is that 0.041%-4.1%(is as 0.041%-0.41%, 0.41%-4.1%, 0.041%, 0.41% or 4.1%), the mass percentage of described dimethyl sulfoxide (DMSO) is that 1%-10%(is as 1%-5%, 5%-10%, 1%, 5% or 10%), the mass percentage of described ethylene glycol is that 1%-10%(is as 1%-5%, 5%-10%, 1%, 5% or 10%), the mass percentage of described xanthans is that 0.001%-0.005%(is as 0.001%-0.003%, 0.003%-0.005%, 0.001%, 0.003% or 0.005%), the mass percentage of described dispersant is that 1-10%(is as 1%-5%, 5%-10%, 1%, 5% or 10%), surplus is water.
Described dispersant specifically can be sodium lignin sulfonate.
The present invention also protects a kind of method processing the land for growing field crops banana plant that banana blight occurs, and comprises the steps: that described sterilization being caused withered dose of perfusion occurs in the banana plant of banana blight to large Tanaka.
When the diameter of the banana plant of described generation banana blight is less than or equal to 15cm, the position of described perfusion is one, i.e. the false stem of banana plant overhead 25-30cm place, and described in each perfusion position perfusion 10-15mL, sterilization causes withered dose.
When the diameter of the banana plant of described generation banana blight is greater than 15cm, the position of described perfusion is two, i.e. the false stem of banana plant overhead 25-30cm place and false stem overhead 50-60cm place, and described in each perfusion position perfusion 20mL, sterilization causes withered dose.
Described perfusion specifically realizes by punching on the false stem of banana plant.The aperture of described punching specifically can be 2.5cm.The method of described punching specifically can be: make drilling tool and ground be 45 ° of-90 ° of angles, inserts after false stem is about 10-30cm and extracts.When to the false stem of described banana plant, overhead 25-30cm place pours into, the method for described punching specifically can be: make drilling tool and ground be 60 ° of-90 ° of angles, inserts after false stem is about 25-30cm and extracts.When to the false stem of described banana plant, overhead 50-60cm place pours into, the method for described punching specifically can be: make drilling tool and ground be 45 ° of-60 ° of angles, inserts after false stem is about 10-15cm and extracts.
The present invention also protects described sterilization to cause the withered dose of application occurred in process in the land for growing field crops banana plant of banana blight.
The present invention also protects described sterilization to cause the withered dose of application in control banana blight.
The present invention also protects a kind of method of preventing and treating banana blight, comprises the step being undertaken by above arbitrary described method by the land for growing field crops banana plant that banana blight occurs processing.
Arbitrary described banana plant specifically can be Brazilian Banana plant above.
Sterilization of the present invention causes in withered dose: select to have the Prochloraz of obvious inhibition to imperfect fungus and sac fungi disease, carbendazim is effective sterilization component, composite rear bactericidal activity strengthens, existing efficiently contact, there is again certain systemic action, drug-fast generation can be avoided simultaneously; Selecting inner sucting conduction type steriland herbicide glyphosate for causing withered composition, can in 10-20 days, diseased plant be dried up, significantly shorten compared to the time of naturally withering of 2-4 month; Select dimethyl sulfoxide (DMSO), ethylene glycol as hydrotropy composition, can permeability be strengthened; Xanthans has suspension, emulsification, the effect such as stable; Dispersant also can strengthen the stability after each component mixture, is conducive to sterilization and causes withered dose and play drug effect sustainedly and stably.
Adopt sterilization provided by the invention to cause the land for growing field crops banana plant of withered dose of process generation banana blight, while killing the in-house pathogen of diseased plant, impel diseased plant to dry up within a short period of time, reach the object of being convenient to residual for diseased plant body transfer to destroy.The method of process banana blight land for growing field crops provided by the invention diseased plant, described sterilization is caused withered dose and directly pours into diseased plant, little by such environmental effects, instant effect, diseased plant of not only can sterilizing on the spot in time, also can greatly shorten diseased plant to dry up the time, be convenient to residual for diseased plant body transfer to destroy, effectively avoid germ to spread in field, environmental pollution is little, other plant is had no adverse effects, time and labour saving.
The invention discloses a kind of sterilization and cause withered dose, and apply the method that described sterilization causes withered dose of process banana blight land for growing field crops diseased plant.The invention solves banana diseased plant biomass huge, not easily sterilize, the time of naturally withering is longer, very easily cause germ to spread in field centered by strain of falling ill spreading and the problem causing large area to fall ill, for the field prevention and control of banana blight provide strong technical support, instant effect, cost is low, effectively can control disease diffusion to spread, reduce the incidence of disease, alleviate economic loss, Social benefit and economic benefit is remarkable, is very suitable for field and applies.
Embodiment
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.Test material used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.Banana plant in embodiment is Brazilian Banana (Musa AAA Cavendish subgroup ' Brazil '), also known as Brazilian any of several broadleaf plants.Quantitative test in following examples, all arranges and repeats experiment for three times, results averaged.
41% gyphosate solution: About Monsanto Chemicals.45% prochloraz aqueous emulsion (also known as 450g/L prochloraz aqueous emulsion): Bayer Cropscience Co., Ltd.
Contrast medicament: 41% gyphosate solution lml, 45% prochloraz aqueous emulsion 0.lml and 29ml water are mixed.
The former medicine of Prochloraz (95%), faint yellow solid pulvis, easily close logical agrochemical supply chain Co., Ltd purchased from Guangzhou, CAS No. is 67747-09-5, and molecular weight is 376.67, and structural formula is as follows:
Carbendazim active compound (95%), white or khaki powder, purchased from Nanjing Ai Sen Fine Chemical Co., Ltd, CAS No. is 10605-21-7, and molecular weight is 191.2, and structural formula is as follows:
Glyphosate technicals (95%), non-volatile white solid, purchased from Shanghai Gui Ming trade Co., Ltd, CAS No. is 1071-83-6, and molecular weight is 169, and structural formula is as follows:
Xanthan collagen medicine (98%), the white or pale yellow powder shape of class, purchased from Shanghai Li Qi auxiliary chemicals Co., Ltd, CAS No. is 11138-66-2, molecular weight about 2 × 10
6, structural formula is as follows:
The former medicine of sodium lignin sulfonate (96%), brown ceramic powder, purchased from Pu Zhen bio tech ltd, Shanghai, CAS No. is 8061-51-6, and structural formula is as follows:
Following drilling tool is adopted: get the conventional molten iron pipe in field or reinforcing bar (diameter is about 0.025m, is about 1.000-1.200m), cut sth. askew one end or be polished into cone-shaped in embodiment.
Embodiment 1, sterilization cause the Synthesis and applications of withered dose
KF1 sterilization causes the preparation of withered dose: former for Prochloraz medicine, carbendazim active compound, glyphosate technicals, dimethyl sulfoxide (DMSO), ethylene glycol, xanthan collagen medicine, the former medicine of sodium lignin sulfonate and water are mixed and stirred, the mass percentage of Prochloraz is made to be 0.045%, the mass percentage of carbendazim is 0.05%, the mass percentage of glyphosate is 0.041%, the mass percentage of dimethyl sulfoxide (DMSO) is 1%, the mass percentage of ethylene glycol is 1%, the mass percentage of xanthans is 0.001%, the mass percentage of sodium lignin sulfonate is 1%, and surplus is water.
In late March, 2013, in banana blight proving ground, Danzhou City, Hainan Province, carries out following four groups of process:
Experimental group (random selecting 60 strain banana blight diseased plant): diseased plant diameter being less than or equal to 15cm, overhead at false stem make a call to a hole to bulb center (drilling tool and ground are 60 ° of-90 ° of angles in 25-30cm place, insert after false stem is about 25-30cm and extract), every strain perfusion 10-15mL KF1 sterilization causes withered dose; Diameter is more than or equal to the diseased plant of 15cm, overhead at false stem make a call to a hole to bulb center (drilling tool and ground are 60 ° of-90 ° of angles in 25-30cm place, insert after false stem is about 25-30cm and extract), overhead at false stem make a call to a hole again (drilling tool and ground are 45 ° of-60 ° of angles in 50-60cm place, insert after false stem is about 10-15cm and extract), every strain perfusion 40mL KF1 sterilization causes withered dose (namely each diseased plant has beaten two holes, pours into 20mL KF1 sterilization and cause withered dose in each hole);
Control group-1(random selecting 60 strain banana blight diseased plant): diseased plant diameter being less than or equal to 20cm, at false stem overhead 30cm place injector for medical purpose single-point injection 30mL contrast medicament (composition is shown in the 3rd page, lower same), injection depth is about 10cm); Diameter is greater than to the diseased plant of 20cm, at false stem overhead 30cm place injector for medical purpose two point injection 60mL contrast medicament (injection depth is about 10cm, namely respectively injects 30mL contrast medicaments at 2 of diseased plant symmetry);
Control group-2(random selecting 60 strain banana blight diseased plant): replace KF1 sterilization to cause withered dose, other same experimental group with contrast medicament.
Control group-3(random selecting 60 strain banana blight diseased plant): do not carry out any process.
Timing from perfusion or injection.Within 10th day, get in diseased plant the position of pouring into medicament place or injection medicament place, adopt PDA plating method (in conjunction with Observations On The Spore Morphology) to detect the bacteria containing amount of wherein Fusarium oxysporum Cuba specialized form.In each plant of control group-3, on average every gram of fresh weight contains 1.151 × 10
4cFU Fusarium oxysporum Cuba specialized form.Compared with control group-3, the Fusarium oxysporum Cuba specialized form quantity that experimental group plant every gram of fresh weight contains reduces 98.45%, the Fusarium oxysporum Cuba specialized form quantity that control group-2 plant every gram of fresh weight contains reduces 85.38%, and the Fusarium oxysporum Cuba specialized form quantity that control group-1 plant every gram of fresh weight contains reduces 58.39%.15th day, experimental group plant is withered (namely caused withered rate and reach 100%) all, and the withered rate that causes of control group-1 is 90%, and the withered rate that causes of control group-2 is 93%.Result shows, sterilization provided by the invention causes withered dose and the method that supports the use significantly is better than existing medicament and application method thereof, and field is easy and simple to handle, is easy to spread.
Embodiment 2, sterilization cause the Synthesis and applications of withered dose
KF2 sterilization causes the preparation of withered dose: former for Prochloraz medicine, carbendazim active compound, glyphosate technicals, dimethyl sulfoxide (DMSO), ethylene glycol, xanthan collagen medicine, the former medicine of sodium lignin sulfonate and water are mixed and stirred, the mass percentage of Prochloraz is made to be 0.45%, the mass percentage of carbendazim is 0.5%, the mass percentage of glyphosate is 0.41%, the mass percentage of dimethyl sulfoxide (DMSO) is 5%, the mass percentage of ethylene glycol is 5%, the mass percentage of xanthans is 0.003%, the mass percentage of sodium lignin sulfonate is 5%, and surplus is water.
In early April, 2013, in banana proving ground, Compounds in Dongfang City of Hainan Province, carry out following two groups of process:
Experimental group (random selecting 20 strain diameter is about the banana blight diseased plant of 20cm): overhead at false stem make a call to a hole to bulb center (drilling tool and ground are 60 ° of-90 ° of angles in 25-30cm place, insert after false stem is about 25-30cm and extract), overhead at false stem make a call to a hole again (drilling tool and ground are 45 ° of-60 ° of angles in 50-60cm place, insert after false stem is about 10-15cm and extract), every strain perfusion 40mL KF2 sterilization causes withered dose (namely each diseased plant has beaten two holes, pours into 20mL KF2 sterilization and cause withered dose in each hole);
Control group (random selecting 20 strain diameter is about the banana blight diseased plant of 20cm): do not carry out any process.
Timing from perfusion.Within 10th day, get in diseased plant the position of pouring into medicament place or injection medicament place, adopt PDA plating method (in conjunction with Observations On The Spore Morphology) to detect the bacteria containing amount of wherein Fusarium oxysporum Cuba specialized form.Compared with control group, the Fusarium oxysporum Cuba specialized form quantity that experimental group plant every gram of fresh weight contains reduces 99.57%.15th day, the withered rate that causes of experimental group reached 100%.
Embodiment 3, sterilization cause the Synthesis and applications of withered dose
KF3 sterilization causes the preparation of withered dose: former for Prochloraz medicine, carbendazim active compound, glyphosate technicals, dimethyl sulfoxide (DMSO), ethylene glycol, xanthan collagen medicine, the former medicine of sodium lignin sulfonate and water are mixed and stirred, the mass percentage of Prochloraz is made to be 4.5%, the mass percentage of carbendazim is 5%, the mass percentage of glyphosate is 4.1%, the mass percentage of dimethyl sulfoxide (DMSO) is 10%, the mass percentage of ethylene glycol is 10%, the mass percentage of xanthans is 0.005%, the mass percentage of sodium lignin sulfonate is 10%, and surplus is water.
In late May, 2013, near Xi Lian farm, Danzhou City, Hainan Province in banana ground, carry out following two groups of process:
Experimental group (random selecting 20 strain diameter is about the banana blight diseased plant of 20cm): overhead at false stem make a call to a hole to bulb center (drilling tool and ground are 60 ° of-90 ° of angles in 25-30cm place, insert after false stem is about 25-30cm and extract), overhead at false stem make a call to a hole again (drilling tool and ground are 45 ° of-60 ° of angles in 50-60cm place, insert after false stem is about 10-15cm and extract), every strain perfusion 40mL KF3 sterilization causes withered dose (namely each diseased plant has beaten two holes, pours into 20mL KF3 sterilization and cause withered dose in each hole);
Control group (random selecting 20 strain diameter is about the banana blight diseased plant of 20cm): do not carry out any process.
Timing from perfusion.Within 10th day, get in diseased plant the position of pouring into medicament place or injection medicament place, adopt PDA plating method (in conjunction with Observations On The Spore Morphology) to detect the bacteria containing amount of wherein Fusarium oxysporum Cuba specialized form.Compared with control group, the Fusarium oxysporum Cuba specialized form quantity that experimental group plant every gram of fresh weight contains reduces 99.62%.15th day, experimental group caused withered rate and reaches 100%.
Claims (8)
1. a sterilization causes withered dose, by Prochloraz, carbendazim, glyphosate, dimethyl sulfoxide (DMSO), ethylene glycol, xanthans, sodium lignin sulfonate and water composition, the mass percentage of described Prochloraz is 0.045-4.5%, the mass percentage of described carbendazim is 0.05-5%, the mass percentage of described glyphosate is 0.041-4.1%, the mass percentage of described dimethyl sulfoxide (DMSO) is 1-10%, the mass percentage of described ethylene glycol is 1-10%, the mass percentage of described xanthans is 0.001-0.005%, the mass percentage of described sodium lignin sulfonate is 1-10%, surplus is water.
2. process a method for the land for growing field crops banana plant that banana blight occurs, comprise the steps: that sterilization according to claim 1 being caused withered dose of perfusion occurs in the banana plant of banana blight to large Tanaka.
3. method as claimed in claim 2, it is characterized in that: the diameter of the banana plant of described generation banana blight is less than or equal to 15cm, the position of described perfusion is one, i.e. the false stem of banana plant overhead 25-30cm place, and described in each perfusion position perfusion 10-15mL, sterilization causes withered dose.
4. method as claimed in claim 2, it is characterized in that: the diameter of the banana plant of described generation banana blight is greater than 15cm, the position of described perfusion is two, the i.e. false stem of banana plant overhead 25-30cm place and false stem overhead 50-60cm place, described in each perfusion position perfusion 20mL, sterilization causes withered dose.
5. as the method as described in arbitrary in claim 2 to 4, it is characterized in that: described perfusion is realized by punching on the false stem of banana plant that banana blight occurs.
6. sterilization according to claim 1 causes the withered dose of application occurred in process in the land for growing field crops banana plant of banana blight.
7. sterilization according to claim 1 causes the withered dose of application in control banana blight.
8. prevent and treat a method for banana blight, comprise the step being undertaken by described method arbitrary in claim 2 to 5 by the land for growing field crops banana plant that banana blight occurs processing.
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| CN110915396A (en) * | 2019-11-30 | 2020-03-27 | 广西金穗农业集团有限公司 | Fertilizing method of blight-resistant banana fertilizer |
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| EP1922927A1 (en) * | 2006-10-26 | 2008-05-21 | Syngeta Participations AG | A method for controlling soybean rust |
| CN101406201A (en) * | 2008-11-25 | 2009-04-15 | 福建省农业科学院植物保护研究所 | Medicament for destroying diseased plant of banana having quarantine Fusarium oxysporum and uses thereof |
| CN101677558A (en) * | 2007-04-25 | 2010-03-24 | 先正达参股股份有限公司 | fungicidal compositions |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP1922927A1 (en) * | 2006-10-26 | 2008-05-21 | Syngeta Participations AG | A method for controlling soybean rust |
| CN101677558A (en) * | 2007-04-25 | 2010-03-24 | 先正达参股股份有限公司 | fungicidal compositions |
| CN101406201A (en) * | 2008-11-25 | 2009-04-15 | 福建省农业科学院植物保护研究所 | Medicament for destroying diseased plant of banana having quarantine Fusarium oxysporum and uses thereof |
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