CN103461127A - Rapid propagation method for gardenia jasminoides - Google Patents
Rapid propagation method for gardenia jasminoides Download PDFInfo
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- CN103461127A CN103461127A CN 201310423762 CN201310423762A CN103461127A CN 103461127 A CN103461127 A CN 103461127A CN 201310423762 CN201310423762 CN 201310423762 CN 201310423762 A CN201310423762 A CN 201310423762A CN 103461127 A CN103461127 A CN 103461127A
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- fructus gardeniae
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Abstract
The invention discloses a rapid propagation method for gardenia jasminoides. The rapid propagation method comprises the steps of sterile seedling acquisition, proliferation culture, rooting culture, acclimatization and transplant, field planting management, and the like. The gardenia jasminoides obtained by the method disclosed by the invention has stable genetic traits and high yield; a large number of seedlings can be obtained in a short period; the period can be shortened; large-scale large field production can be performed.
Description
Technical field
The present invention relates to the quick-breeding method of Plant Tissue Breeding, belong to the plant technology field.
Background technology
Fructus gardeniae is Rubiaceae Gardenia Ellis plant cape jasmine (Gardeni-a jasminoidesEills), often be born in warm sparse woods or barren hill, ditch by, roadside, be distributed in the ground such as Jiangsu, Zhejiang, Anhui, Jiangxi, Yunnan, Guizhou, Sichuan, Hubei, Fujian, Taiwan.With mellow fruit people medicine, treatment pyreticosis vexed, irascibility hot eyes, headache, damp and hot yellow infantile malnutrition due to digestive disturbances or intestinalparasites, stranguria, bloody flux hematuria, aphthae, sore swollen toxin, sprain and swell and ache, be again the important source material of producing plant pigment.The breeding of the methods such as available seed, cuttage, grafting, plant division, but the raising technology of each method is imperfection all, need after fine-variety breeding to keep good characteristic and expanding propagation, be beneficial to enlarge and promote, the Fast-propagation that uses tissue culture mode to carry out fructus gardeniae can solve this difficult problem.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of preparation of fructus gardeniae method for quickly breeding, can provide seedling for the implant mass fructus gardeniae by the method, and can keep the genetic stability of breeding.
Technical problem to be solved by this invention realizes by following scheme:
Get the fructus gardeniae seed, first put into water and soak and gently rub, with 50 ℃ of water seed soakings 36 hours, put into the fine sand that high-temperature sterilization is crossed, after one week, grow aseptic seedling, the aseptic seedling of acquisition cuts root, adds inducing culture B
5+ NAA0.4mg/L+ZT1mg/L+15g/LPVP+3g/LGA
3the cultivation of rising in value, 20 ℃ of temperature, illumination 3000lx, humidity 50%-60%, propagation is cultivated after 30 days and is added root media 3/4LS+0.6mg/LNAA to carry out root induction, 30 ℃ of temperature, illumination 4000lx, humidity 50%-60%, add the plant delayer B of MS medium supplemented 30mg/L when cauline leaf grows to 5cm
9condition of culture: 20 ℃ of temperature, illumination 4000lx.2-3 days before transplanting, seedling is moved to greenhouse together with blake bottle by culturing room, and do shading treatment, sealed membrane is opened, after hardening one week, seedling cauline leaf color burn, the color of root becomes yellowish-brown by yellow-white and is transplanted in aseptic pearlite interstitial substance, in hot-house culture after one week, the plantation of transplanting land for growing field crops, the acid soil of soil pH value 5～6 is advisable, and is disease prevention, the carbendazim solution that periodically sprinkle one time is 600 times.
The fructus gardeniae test-tube plantlet growing way that adopts the present invention to regulate and control out is good, survival rate 94%, and the cycle is short, operates controlledly, can carry out field and plants on a large scale or be applicable.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiment.
Embodiment
Embodiment 1
Get the fructus gardeniae seed, first put into water and soak and gently rub, with 50 ℃ of water seed soakings 36 hours, put into the fine sand that high-temperature sterilization is crossed, after one week, grow aseptic seedling, the aseptic seedling of acquisition cuts root, adds inducing culture B
5+ NAA0.2mg/L+ZT0.5mg/L+15g/LPVP+3g/LGA
3the cultivation of rising in value, 20 ℃ of temperature, illumination 3000lx, humidity 50%-60%, propagation is cultivated after 30 days and is added root media 1/4LS+0.2mg/LNAA to carry out root induction, 30 ℃ of temperature, illumination 4000lx, humidity 50%-60%, add the plant delayer B of MS medium supplemented 30mg/L when cauline leaf grows to 5cm
9condition of culture: 20 ℃ of temperature, illumination 4000lx, 2-3 days before transplanting, seedling is moved to greenhouse together with blake bottle by culturing room, and do shading treatment, sealed membrane is opened, after hardening one week, seedling cauline leaf color burn, the color of root becomes yellowish-brown by yellow-white and is transplanted in aseptic pearlite interstitial substance, in hot-house culture after one week, the plantation of transplanting land for growing field crops, the acid soil of soil pH value 5-6 is advisable, and is disease prevention, the carbendazim solution that periodically sprinkle one time is 600 times, survival rate 85%.
Embodiment 2
Get the fructus gardeniae seed, first put into water and soak and gently rub, with 50 ℃ of water seed soakings 36 hours, put into the fine sand that high-temperature sterilization is crossed, after one week, grow aseptic seedling, the aseptic seedling of acquisition cuts root, adds inducing culture B
5+ NAA0.6mg/L+ZT1.5mg/L+15g/LPVP+3g/LGA
3the cultivation of rising in value, 20 ℃ of temperature, illumination 3000lx, humidity 50%-60%, propagation is cultivated after 30 days and is added root media 3/4LS+1mg/LNAA to carry out root induction, 30 ℃ of temperature, illumination 4000lx, humidity 50%-60%, add the plant delayer B of MS medium supplemented 30mg/L when cauline leaf grows to 5cm
9condition of culture: 20 ℃ of temperature, illumination 4000lx, 2-3 days before transplanting, seedling is moved to greenhouse together with blake bottle by culturing room, and do shading treatment, sealed membrane is opened, after hardening one week, seedling cauline leaf color burn, the color of root becomes yellowish-brown by yellow-white and is transplanted in aseptic pearlite interstitial substance, in hot-house culture after one week, the plantation of transplanting land for growing field crops, the acid soil of soil pH value 5-6 is advisable, and is disease prevention, the carbendazim solution that periodically sprinkle one time is 600 times, survival rate 90%.
Embodiment 3
Get the fructus gardeniae seed, first put into water and soak and gently rub, with 50 ℃ of water seed soakings 36 hours, put into the fine sand that high-temperature sterilization is crossed, after one week, grow aseptic seedling, the aseptic seedling of acquisition cuts root, adds inducing culture B
5+ NAA0.4mg/L+ZT0.5mg/L+15g/LPVP+3g/LGA
3the cultivation of rising in value, 20 ℃ of temperature, illumination 3000lx, humidity 50%-60%, propagation is cultivated after 30 days and is added root media 1/2LS+0.2mg/LNAA to carry out root induction, 30 ℃ of temperature, illumination 4000lx, humidity 50%-60%, add the plant delayer B of MS medium supplemented 30mg/L when cauline leaf grows to 5cm
9condition of culture: 20 ℃ of temperature, illumination 4000lx, 2-3 days before transplanting, seedling is moved to greenhouse together with blake bottle by culturing room, and do shading treatment, sealed membrane is opened, after hardening one week, seedling cauline leaf color burn, the color of root becomes yellowish-brown by yellow-white and is transplanted in aseptic pearlite interstitial substance, in hot-house culture after one week, the plantation of transplanting land for growing field crops, the acid soil of soil pH value 5-6 is advisable, and is disease prevention, the carbendazim solution that periodically sprinkle one time is 600 times, survival rate 87%.
Embodiment 4
Get the fructus gardeniae seed, first put into water and soak and gently rub, with 50 ℃ of water seed soakings 36 hours, put into the fine sand that high-temperature sterilization is crossed, after one week, grow aseptic seedling, the aseptic seedling of acquisition cuts root, adds inducing culture B
5+ NAA0.6mg/L+ZT1mg/L+15g/LPVP+3g/LGA
3the cultivation of rising in value, 20 ℃ of temperature, illumination 3000lx, humidity 50%-60%, propagation is cultivated after 30 days and is added root media 1/4LS+1mg/LNAA to carry out root induction, 30 ℃ of temperature, illumination 4000lx, humidity 50%-60%, add the plant delayer B of MS medium supplemented 30mg/L when cauline leaf grows to 5cm
9condition of culture: 20 ℃ of temperature, illumination 4000lx, 2-3 days before transplanting, seedling is moved to greenhouse together with blake bottle by culturing room, and do shading treatment, sealed membrane is opened, after hardening one week, seedling cauline leaf color burn, the color of root becomes yellowish-brown by yellow-white and is transplanted in aseptic pearlite interstitial substance, in hot-house culture after one week, the plantation of transplanting land for growing field crops, the acid soil of soil pH value 5～6 is advisable, and is disease prevention, the carbendazim solution that periodically sprinkle one time is 600 times, survival rate 89%.
Claims (7)
1. the method for quickly breeding of a fructus gardeniae, its step is as follows:
(1) acquisition of aseptic seedling: get the fructus gardeniae seed, put into water and turn out aseptic seedling;
(2) propagation is cultivated: cut the aseptic seedling root, add inducing culture B
5+ NAA0.4mg/L+ZT1mg/L+15g/LPVP+3g/LGA
3breed cultivation;
(3) culture of rootage: step (2) propagation is cultivated after 30 days and is added root media 3/4LS+0.6mg/LNAA to carry out root induction;
(4) acclimatization and transplants carries out large-scale culture to land for growing field crops.
2. the method for quickly breeding of a kind of fructus gardeniae according to claim 1, it is characterized in that: the acquisition of aseptic seedling is to get the fructus gardeniae seed, first puts into water and soaks and gently rub, with 50 ℃ of water seed soakings 36 hours, put into the fine sand that high-temperature sterilization is crossed, after one week, grow aseptic seedling.
3. the method for quickly breeding of a kind of fructus gardeniae according to claim 1, is characterized in that: proliferated culture medium employing B
5+ NAA0.2-0.6mg/L+ZT0.5-1.5mg/L+15g/LPVP+3g/LGA
3cultivated 20 ℃ of temperature, illumination 3000lx, humidity 50%-60%.
4. the method for quickly breeding of a kind of fructus gardeniae according to claim 1, it is characterized in that: root media is that 1/4-3/4LS+0.2-1mg/LNAA carries out root induction, 30 ℃ of temperature, illumination 4000lx, humidity 50%-60%.
5. the method for quickly breeding of a kind of fructus gardeniae according to claim 1, is characterized in that: the plant delayer B of acclimatization and transplants MS medium supplemented 30mg/L
9, condition of culture: 20 ℃ of temperature, illumination 4000lx.
6. the method for quickly breeding of a kind of fructus gardeniae according to claim 1, it is characterized in that: 2-3 days before transplanting, seedling is moved to greenhouse together with blake bottle by culturing room, and do shading treatment, sealed membrane is opened, after hardening one week, seedling cauline leaf color burn, the color of root becomes yellowish-brown by yellow-white and is transplanted in aseptic pearlite interstitial substance.
7. the method for quickly breeding of a kind of fructus gardeniae according to claim 1 is characterized in that: the acid soil of field plantation management soil pH value 5-6 is advisable, and is disease prevention, the carbendazim solution that periodically sprinkle one time is 600 times.
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| CN 201310423762 CN103461127A (en) | 2013-09-17 | 2013-09-17 | Rapid propagation method for gardenia jasminoides |
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| CN 201310423762 CN103461127A (en) | 2013-09-17 | 2013-09-17 | Rapid propagation method for gardenia jasminoides |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069784A (en) * | 2016-08-10 | 2016-11-09 | 福建省农业科学院果树研究所 | A kind of method for tissue culture of Flos Caryophylli Fructus Gardeniae petal |
| CN106171880A (en) * | 2016-06-28 | 2016-12-07 | 广西南宁十分园生态农业科技有限公司 | A kind of SHANZHIZI cuttage and seedling culture method |
| CN114793905A (en) * | 2022-05-23 | 2022-07-29 | 浙江省亚热带作物研究所(浙南林业科学研究院) | Rapid propagation method and culture medium for gardenia tissue culture seedlings |
| CN116616179A (en) * | 2023-06-02 | 2023-08-22 | 江西省林业科学院 | Tissue culture rapid propagation method of excellent clone of Gardenia jasminoides ellis with high content of geniposide |
-
2013
- 2013-09-17 CN CN 201310423762 patent/CN103461127A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106171880A (en) * | 2016-06-28 | 2016-12-07 | 广西南宁十分园生态农业科技有限公司 | A kind of SHANZHIZI cuttage and seedling culture method |
| CN106069784A (en) * | 2016-08-10 | 2016-11-09 | 福建省农业科学院果树研究所 | A kind of method for tissue culture of Flos Caryophylli Fructus Gardeniae petal |
| CN114793905A (en) * | 2022-05-23 | 2022-07-29 | 浙江省亚热带作物研究所(浙南林业科学研究院) | Rapid propagation method and culture medium for gardenia tissue culture seedlings |
| CN116616179A (en) * | 2023-06-02 | 2023-08-22 | 江西省林业科学院 | Tissue culture rapid propagation method of excellent clone of Gardenia jasminoides ellis with high content of geniposide |
| CN116616179B (en) * | 2023-06-02 | 2024-02-13 | 江西省林业科学院 | Tissue culture rapid propagation method of excellent clone of Gardenia jasminoides ellis with high content of geniposide |
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Application publication date: 20131225 |