CN103444942B - Method of preparing theabrownine with green teas as raw materials - Google Patents
Method of preparing theabrownine with green teas as raw materials Download PDFInfo
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- CN103444942B CN103444942B CN201210325200.5A CN201210325200A CN103444942B CN 103444942 B CN103444942 B CN 103444942B CN 201210325200 A CN201210325200 A CN 201210325200A CN 103444942 B CN103444942 B CN 103444942B
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Abstract
A method of preparing theabrownine with green teas as raw materials is provided. The method comprises the following steps: with a certain mass of green teas as raw materials, spraying water containing 0.2-8.0% of edible acetic acid on the surfaces of the green tea leaves, wherein the amount of the sprayed water is 5.0-40.0% of the mass of the tea leaves; then placing the green tea materials subjected to water spraying in a double-screw extrusion-expansion machine for expansion treatment, wherein the temperatures of four extrusion sections of the inlets and the outlets of the double-screw extrusion-expansion machine are controlled to be 20-35 DEG C, 50-70 DEG C, 80-120 DEG C and 120-180 DEG C respectively and the rotation speed of the screw is controlled to be 50-200 r/min; adding water in the expended tea leaves, wherein the adding amount of the water is 3-100 times as much as mass of the expended tea leaves; extracting for 0.5-12 hours at the temperature of 40-98 DEG C; filtering with gauze to obtain filtrate; filtering the filtrate in a micro-filtration membrane with an interception hole size of 0.25-10 mu m to obtain a microfiltration filtrate; filtrating the microfiltration filtrate in an ultrafiltration membrane with interception molecular weight of 1000-20000Da to obtain an ultrafiltration filtrate; concentrating the ultrafiltration filtrate in a nanofiltration reverse osmosis membrane or rotary evaporation to obtain a concentrated liquid; carrying out spray drying or vacuum freeze drying on concentrated liquid to obtain the theabrownine.
Description
Technical field
The present invention relates to tea comprehensive processing and biological chemical field, be related specifically to a kind of method adopting twin-screw extruder expanding treatment binding film isolation technics to prepare theabrownin.
Background technology
Theabrownin is that the compound based on tea leaf polyphenols is polymerized the molecule amount heterogeneity, the water miscible complicated natural high polymer that produce under heating power and non-heat effect through deep oxidation, is the characteristic functional biochemistry composition of the black tea such as Pu'er tea, the black tea in Anhua, six fort tea.Black tea is to health health-care efficacy and dark brownly have significant correlation, if effectively reduce blood fat, blood pressure, has remarkable prevention effect in cardiovascular and cerebrovascular disease, can be used as the function raw material of food and health products.The technology of preparing of current theabrownin is less.
China is green tea plantation, processing and big trading nation.The green tea that the polyphenol content of autumn in summer green tea was gathered and processed apparently higher than other times, because of Tea Polyphenols bitter and puckery flavor, it can not get market favor as processing raw material of conventional green tea always, this also causes China's Summer-autumn tea leaf picking rate low, especially in green tea producing region, tea fresh leaves living resources utilization rate is not high, but tea place fertilising in early stage and plant protection measure input amount are greatly, cause significant wastage.If with autumn in summer green tea for raw material, expand the deep processing and utilization approach of green tea, this sustainable development for tea industry is significant.
Summary of the invention
For solving the problem, the invention provides and a kind of adopt physical processing techniques and twin-screw extruder expanding treatment and in conjunction with Using Multistage Membranes isolation technics, with each grade green tea for raw material, carry out heating power oxidation reaction transform and extraction and isolation prepare theabrownin, its concrete steps are as follows:
(1) with the green tea of certain mass for raw material, the water containing mass percent concentration being 0.2-8.0% alimentary acetic acid is sprayed in green tea surface, injection flow rate is 5.0 ~ 40.0% of tea quality, again green tea material of this water spray process is placed in twin-screw extruder, controls bulking machine entrance and be respectively (20-35) DEG C, (50-70) DEG C, (80-120) DEG C and (120-180) DEG C to outlet four extruded segment temperature; Screw rod rotary speed 50-200r/min, carries out twin-screw extruder expanding treatment.
(2) by the tealeaves of above-mentioned twin-screw extruder expanding treatment, add its quality 3-100 water doubly, extract 0.5-12 hour in 40-98 DEG C, then with gauze or strainer filtering, obtain filtrate.
(3) filtrate in described step (2) is first through retaining the micro-filtrate membrane filtration that aperture is 0.25-10um, and micro-filtration filtrate is the ultrafiltration membrance filter of 1000-20000Da through molecular cut off again, obtains ultrafiltrate.
(4) through nanofiltration reverse osmosis membrane, concentrated or rotary evaporation concentrates the ultrafiltrate in described step (3), obtains concentrate.
(5) in described step (4), concentrate obtains theabrownin through spraying dry or vacuum freeze drying.
Described green tea is the green tea of each grade, the green tea of the tea fresh leaves processing of especially plucking in 6-8 month in summer or 9-11 month in autumn.
Novelty of the present invention and practical function are: adopt twin-screw extruder puffing technique process green tea, controlled, make the Tea Polyphenols development of heat in green tea and non-heating power oxidative polymerization, be converted into theabrownin by the procedural temperature in processing procedure.Simultaneously, twin-screw extruder expanding treatment can make tealeaves tissue and eucaryotic cell structure effectively broken, thus improve the release rate (yield) of theabrownin in subsequent extracted process, in conjunction with Using Multistage Membranes filtration and separation techniques, whole preparation process does not use any organic chemistry to extract reagent, therefore product organic solvent-free remains, preparation process environmental protection.
Detailed description of the invention
Embodiment 1:
The roasted green tea gathered and processed with 800 grams of Maies is raw material, water containing 0.4% alimentary acetic acid is sprayed in green tea surface, injection flow rate is 36.0% of tea quality, again the green tea material of this water spray process is placed in twin-screw extruder, control its entrance and be respectively 23 DEG C, 55 DEG C, 85 DEG C and 125 DEG C to outlet four extruded segment temperature, screw rod rotary speed 60r/min, carries out expanding treatment.In tealeaves after this expanding treatment, add the water of its quality 8 times, extract 0.5 hour in 45 DEG C, then use filtered through gauze, obtain filtrate.By filtrate through retaining the micro-filtrate membrane filtration that aperture is 0.30um, obtain micro-filtrate, micro-filtrate is the ultrafiltration membrance filter of 1000Da through molecular cut off again, obtains ultrafiltrate.Ultrafiltrate concentrates through nanofiltration reverse osmosis membrane, and obtain concentrate, concentrate obtains theabrownin 76 grams through vacuum freeze drying.
Embodiment 2:
The dark green tea of the solarization of gathering and processing with 5000 grams of Julies is raw material, water containing 4.0% alimentary acetic acid is sprayed in green tea surface, injection flow rate is 15.0% of tea quality, again the green tea material of this water spray process is placed in twin-screw extruder, control its entrance and be respectively 28 DEG C, 60 DEG C, 100 DEG C and 155 DEG C to outlet four extruded segment temperature, screw rod rotary speed 100r/min, carries out expanding treatment.In tealeaves after this expanding treatment, add the water of its quality 30 times, extract 3.5 hours in 78 DEG C, then use strainer filtering, obtain filtrate.By filtrate through retaining the micro-filtrate membrane filtration that aperture is 2.5um, obtain micro-filtrate, micro-filtrate is the ultrafiltration membrance filter of 7000Da through molecular cut off again, obtains ultrafiltrate.Ultrafiltrate concentrates through rotary evaporation, and obtain concentrate, concentrate obtains theabrownin 650 grams through spraying dry.
Embodiment 3:
The baked green tea gathered and processed with 2000 grams of Septembers is raw material, water containing 7.4% alimentary acetic acid is sprayed in green tea surface, injection flow rate is 5.0% of tea quality, again the green tea material of this water spray process is placed in twin-screw extruder, control its entrance and be respectively 32 DEG C, 70 DEG C, 115 DEG C and 175 DEG C to outlet four extruded segment temperature, screw rod rotary speed 180r/min, carries out expanding treatment.In tealeaves after this expanding treatment, add the water of its quality 70 times, extract 10.5 hours in 95 DEG C, then use filtered through gauze, obtain filtrate.By this filtrate through retaining the micro-filtrate membrane filtration that aperture is 7.5um, obtain micro-filtrate, micro-filtrate is the ultrafiltration membrance filter of 15000Da through molecular cut off again, obtains ultrafiltrate.Ultrafiltrate concentrates through nanofiltration reverse osmosis membrane, and obtain concentrate, concentrate obtains theabrownin 290 grams through vacuum freeze drying.
Claims (1)
1. be the method that theabrownin prepared by raw material with green tea, it is characterized in that following step:
(1) with the green tea of certain mass for raw material, the water containing mass percent concentration being 0.2-8.0% alimentary acetic acid is sprayed in green tea surface, injection flow rate is 5.0 ~ 40.0% of tea quality, again the green tea material of this water spray process is placed in twin-screw extruder, control bulking machine entrance and be respectively (20-35) DEG C, (50-70) DEG C, (80-120) DEG C and (120-180) DEG C to outlet four extruded segment temperature, screw rod rotary speed 50-200r/min, carries out twin-screw extruder expanding treatment;
(2) tealeaves of twin-screw extruder expanding treatment that obtains of step (1), adds its quality 3-100 water doubly, extracts 0.5-12 hour, then with gauze or strainer filtering, obtain filtrate in 40-98 DEG C;
(3) filtrate in described step (2) is first through retaining the micro-filtrate membrane filtration that aperture is 0.25-10 μm, and micro-filtration filtrate is the milipore filter of 1000-20000Da through molecular cut off again, obtains ultrafiltrate;
(4) ultrafiltrate in described step (3) concentrates through nanofiltration reverse osmosis concentration or rotary evaporation, obtains concentrate;
(5) in described step (4), concentrate obtains theabrownin through spraying dry or freeze drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210325200.5A CN103444942B (en) | 2012-09-05 | 2012-09-05 | Method of preparing theabrownine with green teas as raw materials |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210325200.5A CN103444942B (en) | 2012-09-05 | 2012-09-05 | Method of preparing theabrownine with green teas as raw materials |
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| CN103444942A CN103444942A (en) | 2013-12-18 |
| CN103444942B true CN103444942B (en) | 2015-05-06 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104193844A (en) * | 2014-09-07 | 2014-12-10 | 陈小强 | Method for preparing theabrownin and low-molecular-weight tea polysaccharides by fermenting green tea as raw material |
| CN105925490A (en) * | 2016-06-03 | 2016-09-07 | 云南农业大学 | Aspergillus tubingensis and method for producing theabrownins through liquid fermentation of Aspergillus tubingensis |
| CN106167765A (en) * | 2016-06-03 | 2016-11-30 | 云南农业大学 | A kind of method that Tabin aspergillus and liquid fermentation thereof produce abrownin |
| CN107997185B (en) * | 2017-12-20 | 2021-03-30 | 福州大学 | Method for synchronously preparing tea residue functional peptide and tea essence and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1074618A (en) * | 1993-01-01 | 1993-07-28 | 南昌洪婺名茶开发公司 | Tea pigment and production method thereof |
| CN1602711A (en) * | 2004-11-01 | 2005-04-06 | 杨贤强 | Instant gas differential pressure method for processing tea leaf |
| CN101961422A (en) * | 2009-07-24 | 2011-02-02 | 天津天士力现代中药资源有限公司 | Extractive of Pu'er tea and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1074618A (en) * | 1993-01-01 | 1993-07-28 | 南昌洪婺名茶开发公司 | Tea pigment and production method thereof |
| CN1602711A (en) * | 2004-11-01 | 2005-04-06 | 杨贤强 | Instant gas differential pressure method for processing tea leaf |
| CN101961422A (en) * | 2009-07-24 | 2011-02-02 | 天津天士力现代中药资源有限公司 | Extractive of Pu'er tea and preparation method thereof |
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