CN103444306A - Method for quarantining imported sugarcane seeds - Google Patents
Method for quarantining imported sugarcane seeds Download PDFInfo
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- CN103444306A CN103444306A CN2013103888706A CN201310388870A CN103444306A CN 103444306 A CN103444306 A CN 103444306A CN 2013103888706 A CN2013103888706 A CN 2013103888706A CN 201310388870 A CN201310388870 A CN 201310388870A CN 103444306 A CN103444306 A CN 103444306A
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Abstract
The invention provides a method for quarantining imported sugarcane seeds. The method comprises the following steps: firstly, carrying out visual inspection on the seeds; if insects are found, carrying out stifling on the seeds or destroying the seeds; after the visual inspection, carrying out sterilization and insect-killing treatment on the sugarcane seeds; sowing the treated seeds into a sterilized seedling growing base material; placing the base material into a special sugarcane quarantine greenhouse to carry out isolated observation for more than three months; after the three months, collecting leaves to carry out PCR ((Polymerase Chain Reaction) detection on sugarcane Fiji diseases, mosaic diseases and bacterial blights; destroying detected positive materials immediately; transplanting detected negative materials into disinfected pot cultivation soil to carry out epidemic surveillance within a whole growth period; during the surveillance process, observing quarantine plant diseases and insect pests of a sugarcane every one month; after the quarantine is finished, harvesting sugarcane stems which do not have the quarantine plant diseases and insect pests and can be used for reproduction tests and researches. With the adoption of the method, the imported sugarcane seeds are subjected to the quarantine treatment so as to effectively prevent the dangerous plant diseases and insect pests from spreading to China along with the imported seeds to cause dangers.
Description
Technical field
The invention belongs to the plant protection technology field, be specifically related to a kind of sugarcane import seed quarantine method, be exclusively used in sugarcane import seed quarantine.
Background technology
It is quick, a convenient utilization up-to-date breeding achievement all over the world that sugarcane is introduced a fine variety, the effective way of improvement sugar cane breed structure and increase genetic resources, but also increased dangerous plant pests and imported China into Soybean germplasm introduction, the potential risk that China's sugar industry is brought about great losses simultaneously.How to prevent that dangerous plant pests from importing China's sugarcane district into by introducing a fine variety, the strict quarantine precautionary measures of introducing a fine variety that this just need to take to perfect, guarantee to introduce a fine variety the not dangerous damage by disease and insect of material.
In addition, along with deepening continuously of international sugarcane germplasm exchange work, exchange material not only is confined to sugarcane sugarcane stem and has expanded to the exchange of sugarcane seed, many important sugarcane damage by disease and insect are all by seed dispersal in addition, as the smut in fungal disease, ear rot and systemic viral viral disease mosaic disease and phytoplasma disease hoja blanca, also have sugarcane mite class etc. all easily by seed dispersal, cause just constantly increasing by introducing a fine variety the damage by disease and insect propagation risk caused.And sugarcane import seed quarantine method is studied and set up to the current domestic quarantine method there is no for sugarcane import seed therefore, become and effectively prevent that dangerous plant pests from importing by introducing the sugarcane seed key that harm is spread in China's sugarcane district into.
Summary of the invention
The present invention is directed to culmicolous smut of sugar cane, ear rot and systemic viral viral disease mosaic disease and phytoplasma disease hoja blanca, also has the dangerous plant pests such as sugarcane mite class easily by the characteristic of seed dispersal, a kind of sugarcane import seed quarantine method is provided, thereby has guaranteed the safe sustainable development of China's sugar industry and carrying out smoothly of international cooperation sugarcane seed material exchange.
The present invention quarantines to introducing the sugarcane seed by the following method:
After receiving seed, first seed is carried out to visual inspection, if find that there is insect, material should be fumigated and process or destroy; After visual inspection, for effectively killing culmicolous smut of sugar cane, ear rot and systemic viral viral disease mosaic disease and the phytoplasma disease hoja blanca that may carry, also have sugarcane mite class worm's ovum etc., must adopt broad spectrum activity sterilization and disinsection miticide to carry out the sterilizing pesticide processing to the sugarcane seed; Seed after processing should be sowed in the seedling medium after sterilization, and is placed in the special-purpose quarantine of sugarcane greenhouse and is quarantined, and observes more than 3 months; After 3 months, gather blade systemic viral viral disease Fiji disease, mosaic disease and phytoplasma disease hoja blanca are carried out to RT-PCR and nest-type PRC detection; If tests positive, all material must be destroyed immediately, if detect, is negative, and seedling is transplanted in the barrel plant soil after sterilization, and carries out the epidemic monitoring of a complete growth cycle; During monitoring, every 1 month, by an experienced virologist, whether the sugarcane strain is occurred to the quarantine damage by disease and insect is checked; After quarantine finishes, results do not detect and do not observe the sugarcane stem of quarantine damage by disease and insect and study for reproduction test.
The invention has the beneficial effects as follows a kind of normalized sugarcane import seed quarantine method that meets international standard of having set up of studying, utilize the inventive method, import sugarcane seed is carried out to quarantine and the epidemic monitoring of a growth cycle, can effectively prevent that dangerous venereal disease worm from importing into and spreading harm with the import seed, for China's sugarcane germplasm exchange work, be provided in order smoothly technical guarantee.
Embodiment
(1) after receiving seed, first carry out visual inspection, if find that there is insect, material should be fumigated and process or destroy.
(2) after visual inspection, the culmicolous smut of sugar cane that may carry for effectively killing import sugarcane seed, ear rot and systemic viral viral disease mosaic disease and phytoplasma disease hoja blanca, also has sugarcane mite class worm's ovum etc., must adopt broad spectrum activity sterilization and disinsection miticide 500 times of liquid of 50% thiram wettable powder add 800 times of liquid of 50% carbendazol wettable powder and add 1500 times of immersion kind 5-10min of 5% Hexythiazox missible oil, use again the vexed 6h of plastic sack, in the seedling medium of planting seed after processing after sterilization, (seedling medium is due to fertilizer, soil and vermiculite mix, fertilizer is 1/5 in mass ratio: soil is 3/5: vermiculite is 1/5), and be placed in the special-purpose quarantine of sugarcane greenhouse and quarantined, observe more than 3 months.
After (3) 3 months, gather blade systemic viral viral disease Fiji disease, mosaic disease and phytoplasma disease hoja blanca are carried out to RT-PCR and nest-type PRC detection; If tests positive, all material must be destroyed immediately, if detect, is negative, and seedling is transplanted in the barrel plant soil after sterilization, and carries out the epidemic monitoring of a complete growth cycle.
(4) during the monitoring, every 1 month, by an experienced virologist, the quarantine damage by disease and insect of sugarcane strain is observed; After quarantine finishes, results do not detect and observe the sugarcane stem of quarantine damage by disease and insect and study for reproduction test.
Claims (3)
1. a sugarcane import seed quarantine method.It is characterized in that: after receiving seed, first seed is carried out to visual inspection, if find that there is insect, material should be fumigated and process or destroy; After visual inspection, for effectively killing culmicolous smut of sugar cane, ear rot and systemic viral viral disease mosaic disease and the phytoplasma disease hoja blanca that may carry, also have sugarcane mite class worm's ovum, adopt broad spectrum activity sterilization and disinsection miticide to carry out the sterilizing pesticide processing to the sugarcane seed; In the seedling medium of planting seed after processing after sterilization, and be placed in the special-purpose quarantine of sugarcane greenhouse and quarantined, observe more than 3 months; After 3 months, gather blade systemic viral viral disease Fiji disease, mosaic disease and phytoplasma disease hoja blanca are carried out to RT-PCR and nest-type PRC detection; If tests positive, all material must be destroyed immediately, if detect, is negative, and seedling is transplanted in the barrel plant soil after sterilization, and carries out the epidemic monitoring of a complete growth cycle; During monitoring, every 1 month, by an experienced virologist, sugarcane strain quarantine damage by disease and insect is observed; After quarantine finishes, results do not detect and do not observe the sugarcane stem of quarantine damage by disease and insect and study for reproduction test.
2. a kind of sugarcane import seed quarantine method according to claim 1, it is characterized in that: the sugarcane seed is carried out to the sterilizing pesticide processing, adopting broad spectrum activity sterilization and disinsection miticide is that 500 times of liquid of 50% thiram wettable powder add 800 times of liquid of 50% carbendazol wettable powder and add 1500 times of immersion kind 5-10min of 5% Hexythiazox missible oil, then uses the vexed 6h of plastic sack.
3. a kind of sugarcane import seed quarantine method according to claim 1, it is characterized in that: to the detection of systemic seed-borne disease, isolate sowing and observe after 3 months, the collection blade is to systemic viral viral disease Fiji disease, mosaic disease and the phytoplasma disease hoja blanca is carried out RT-PCR and nest-type PRC detects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310388870.6A CN103444306B (en) | 2013-08-31 | 2013-08-31 | A kind of sugarcane import seed quarantine method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310388870.6A CN103444306B (en) | 2013-08-31 | 2013-08-31 | A kind of sugarcane import seed quarantine method |
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| CN103444306A true CN103444306A (en) | 2013-12-18 |
| CN103444306B CN103444306B (en) | 2015-11-18 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007057402A1 (en) * | 2005-11-15 | 2007-05-24 | Biogemma | Emp4 gene |
| CN101144793A (en) * | 2007-10-29 | 2008-03-19 | 云南省农业科学院甘蔗研究所 | Bagasse fiji virus detection method |
| CN101906483A (en) * | 2010-07-20 | 2010-12-08 | 云南省农业科学院甘蔗研究所 | Quarantine method of sugarcane seedlings to be exported |
| CN102154490A (en) * | 2011-03-02 | 2011-08-17 | 云南省农业科学院甘蔗研究所 | Pathogenic nested polymerase chain reaction (PCR) detection method for sugarcane white leaf diseases |
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2013
- 2013-08-31 CN CN201310388870.6A patent/CN103444306B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007057402A1 (en) * | 2005-11-15 | 2007-05-24 | Biogemma | Emp4 gene |
| CN101144793A (en) * | 2007-10-29 | 2008-03-19 | 云南省农业科学院甘蔗研究所 | Bagasse fiji virus detection method |
| CN101906483A (en) * | 2010-07-20 | 2010-12-08 | 云南省农业科学院甘蔗研究所 | Quarantine method of sugarcane seedlings to be exported |
| CN102154490A (en) * | 2011-03-02 | 2011-08-17 | 云南省农业科学院甘蔗研究所 | Pathogenic nested polymerase chain reaction (PCR) detection method for sugarcane white leaf diseases |
Non-Patent Citations (1)
| Title |
|---|
| 王福祥: "甘蔗种质资源国际交流的一般原则和技术环节", 《甘蔗》, vol. 2, no. 1, 31 January 1995 (1995-01-31) * |
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