CN103430913A - Method for temporary rearing of alitta succinea by means of agar - Google Patents
Method for temporary rearing of alitta succinea by means of agar Download PDFInfo
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- CN103430913A CN103430913A CN2013103601176A CN201310360117A CN103430913A CN 103430913 A CN103430913 A CN 103430913A CN 2013103601176 A CN2013103601176 A CN 2013103601176A CN 201310360117 A CN201310360117 A CN 201310360117A CN 103430913 A CN103430913 A CN 103430913A
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Abstract
The invention relates to a method for temporary rearing of alitta succinea by means of agar. The method for temporary rearing of the alitta succinea by means of the agar comprises the steps of (1) preparing agar gel, (2) preparing temporary rearing water, (3) setting the temporary rearing environment, and (4) feeding the alitta succinea with bait and changing water. According to the method for temporary rearing of the alitta succinea by means of the agar, sandy soil is replaced by the agar, the transparency property of an agar block is used, and the morphological characteristics and living habits of the alitta succinea in the natural state can be observed.
Description
Technical field
The present invention relates to the halobiontic technology of supporting temporarily, particularly a kind of agar that utilizes is supported the method for perinereis aibihitensis Grube temporarily.
Background technology
Perinereis aibihitensis Grube (Perinereis aibuhitensis) belongs to Annelida, polychaeta, Nereidae, happiness is lived in caves in calm, nutritious silt particle beach, intertidal zone, the sociales of silt particle beach, China intertidal zone biology, be also the main composition biology of CHINESE OFFSHORE bottom community, there is the important ecological value.Clam worm is the high-quality bait of fish, shrimp, crab etc., and nutritive value is abundant, is called as " omnipotent bait ", has high economic worth.The C.I.F. of Japanese market is per ton up to tens thousand of dollars at present, and very worthy equally in market, West Europe, supply falls short of demand.Perinereis aibihitensis Grube has the characteristics such as individuality is large, protein content high (70%-80%), is the important kind that can develop in the Nereidae animal, has wide development prospect.
In recent years, domestic many scientific research institutions and enterprise have carried out the cultural technique research of perinereis aibihitensis Grube in succession.At first perinereis aibihitensis Grube life and physiological habit, as the basic theory of carrying out new cultural technique, should be studied clear.But the behavior of uniting in the happiness sludge sand of perinereis aibihitensis Grube and water body has hindered the morphological feature under its Natural Survival state and the observation of life habit, limited and take the carrying out of its correlative study that is experiment sample.Therefore, how to guarantee that under the normal survival condition of perinereis aibihitensis Grube, observing its morphological feature and life habit becomes the technical barrier that always perplexs scientific and technical personnel.The present invention has aimed to provide the method for temporarily cultivating of a kind of simulating nature state observation perinereis aibihitensis Grube form and life habit.
Perinereis aibihitensis Grube can be united, and can't carry out independent morphologic observation, and the survival condition in its water body can't embody the life habit of perinereis aibihitensis Grube in sandy soil really.
Summary of the invention
For the defect existed in prior art, the present invention has aimed to provide the method for temporarily cultivating of a kind of simulating nature state observation perinereis aibihitensis Grube form and life habit.
A kind of agar that utilizes is supported the method for perinereis aibihitensis Grube temporarily, and concrete grammar is as follows:
1) preparation of agar gel:
Agar powder is dissolved in temporarily and supports in water, and heating is boiled, and agar is dissolved, and the agar gel intensity of making is 400g/cm
2, afterwards, agar gel being sub-packed in the glass beaker of 500ml, in each beaker, the amount of agar gel is 300ml, before packing, glass beaker is soaked to 24h with 1% watery hydrochloric acid, with clear water, cleans up afterwards;
2) support temporarily the preparation of water:
Take from right seawater and staticly settle 7 days, adopt the fibrous filter membrane that aperture is 45 μ m to filter, carry out high-temperature sterilization (121 ℃, 20min) after filtration, cool to room temperature is standby; Take from water and be placed on the sufficient place of well-ventilated, illumination aeration 7 days, afterwards nature seawater and running water are allocated to salinity be 26PSU, finally add BZT(nitre ammonia), making its concentration is 0.3PPM, makes and supports temporarily water;
The nature seawater source quality meets national water quality standard for fishery (GB11607), stores up water quality and meets pollution-free food-mariculture water water standard (NY5052).
3) support temporarily the environment setting:
Perinereis aibihitensis Grube is positioned in the glass beaker that agar gel is housed prepared by step 1), and inject 100ml and support temporarily water in beaker, afterwards beaker is put into to biochemical cultivation case, connect oxygen charging pump (extra large sharp AC0-009D) with small size aeration stone, small size aeration stone is placed into to supporting in water temporarily of beaker upper strata, maintains in water body dissolved oxygen content all the time more than 10mg/L;
In biochemical cultivation case, temperature constant is 15 ± 1 ℃, lucifuge;
Wherein in each beaker, support temporarily 30 perinereis aibihitensis Grubes, the adult that perinereis aibihitensis Grube is 1-4cm;
4) bait feeding, water body are changed:
Change every three days and support temporarily water body, with bait feeding, synchronize and carry out; Bait is selected chlorella or Chaetoceros, changes water and the water of supporting temporarily in beaker is all outwelled before throwing something and feeding, add mix fresh algae liquid support temporarily water 100ml, the density of mixed algae liquid is 20/ml;
5) find that agar is broken serious, the timely replacing if support temporarily in process; If find that there is in temporarily foster process, health is imperfect, body surface has rotten clam worm, migrates out immediately beaker.
Illustrate: BZT is probiotics commonly used in the production of U.S. BIO-FormL.L.C. (Bi Wofeng biotech firm) company, mariculture, and it is proportionally configured and form by microbial composite bacteria+biology enzyme, has the effect at the clear end of water purification.BZT(nitre ammonia) be a kind of in many BZT products, nitrite and the ammonia nitrogen in degrading cultivation water, effectively suppress the breeding growth of noxious bacteria rapidly.
The method of utilizing agar to support temporarily perinereis aibihitensis Grube of the present invention, replace sandy soil with agar, utilizes the characteristic that agar block is transparent, and the technical method of observing perinereis aibihitensis Grube its morphological feature and life habit under nature is provided.
Embodiment
Embodiment 1
A kind of agar that utilizes is supported the method for perinereis aibihitensis Grube temporarily, and concrete grammar is as follows:
1) preparation of agar gel:
Agar powder is dissolved in temporarily and supports in water, and heating is boiled, and agar is dissolved, and the agar gel intensity of making is 400g/cm
2, afterwards, agar gel being sub-packed in the glass beaker of 500ml, in each beaker, the amount of agar gel is 300ml, before packing, glass beaker is soaked to 24h with 1% watery hydrochloric acid, with clear water, cleans up afterwards; Make 8 500ml glass beakers that contain above-mentioned agar gel;
2) support temporarily the preparation of water:
Take from right seawater and staticly settle 7 days, adopt the fibrous filter membrane that aperture is 45 μ m to filter, carry out high-temperature sterilization (121 ℃, 20min) after filtration, cool to room temperature is standby; Take from water and be placed on the sufficient place of well-ventilated, illumination aeration 7 days, afterwards nature seawater and running water are allocated to salinity be 26PSU, finally add BZT(nitre ammonia), making its concentration is 0.3PPM, makes and supports temporarily water; Wherein the nature seawater source quality meets national water quality standard for fishery (GB11607), stores up water quality and meets pollution-free food-mariculture water water standard (NY5052);
3) support temporarily the environment setting:
Choosing 240 is positioned in the glass beaker that agar gel is housed prepared by step 1) Wu Sun Shang ﹑ body colour gloss, the perinereis aibihitensis Grube rapidly of creeping, support 30 temporarily in each beaker, the clam worm body is long is 1-4cm, and inject 100ml and support temporarily water in beaker, afterwards beaker is put into to biochemical cultivation case, connect oxygen charging pump with small size aeration stone, small size aeration stone is placed into to supporting in water temporarily of beaker upper strata, maintain in water body dissolved oxygen content all the time more than 10mg/L;
In biochemical cultivation case, temperature constant is 15 ± 1 ℃, lucifuge;
4) bait feeding, water body are changed:
Change every three days and support temporarily water body, with bait feeding, synchronize and carry out; Bait is selected chlorella or Chaetoceros, changes water and the water of supporting temporarily in beaker is all outwelled before throwing something and feeding, add mix fresh algae liquid support temporarily water 100ml, the density of mixed algae liquid is 20/ml; The situation of throwing something and feeding is transparent in agar block to guarantee in beaker, and water quality is limpid, does not cause waste.
5) find that agar is broken serious, the timely replacing if support temporarily in process; If find that there is in temporarily foster process, health is imperfect, body surface has rotten clam worm, migrates out immediately beaker.
Support temporarily perinereis aibihitensis Grube after 30 days through said method, perinereis aibihitensis Grube survival rate>85%, the individual body colour gloss of surviving, vigor is normal.Support temporarily the flowing of form, blood of the body segment that can observe clearly perinereis aibihitensis Grube in process, parapodum, with and the nest that excavates in agar block and overall process and the lifestyle in tunnel, and then provide technical support for basic research such as physiological habits under research perinereis aibihitensis Grube nature animation.
The present invention utilizes agar block to replace sandy soil, utilizes its transparent characteristics, is beneficial to and observes perinereis aibihitensis Grube morphological feature and life habit.
The mentioned agar block intensity of the present invention is 400g/cm
2, while guaranteeing the beaker inclination, agar block can not break, and clam worm can freely pierce in agar block.
The present invention adopts micro-algae to support temporarily the bait in process as perinereis aibihitensis Grube, is convenient to keep supporting temporarily the transparent of agar in environment, and dissolved oxygen content and water quality in water body are limpid.
Claims (1)
1. one kind is utilized agar to support temporarily the method for perinereis aibihitensis Grube, it is characterized in that concrete grammar is as follows:
1) preparation of agar gel:
Agar powder is dissolved in temporarily and supports in water, and heating is boiled, and agar is dissolved, and the agar gel intensity of making is 400g/cm
2, afterwards, agar gel being sub-packed in the glass beaker of 500ml, in each beaker, the amount of agar gel is 300ml, before packing, glass beaker is soaked to 24h with 1% watery hydrochloric acid, with clear water, cleans up afterwards;
2) support temporarily the preparation of water:
Take from right seawater and staticly settle 7 days, adopt the fibrous filter membrane that aperture is 45 μ m to filter, carry out high-temperature sterilization (121 ℃, 20min) after filtration, cool to room temperature is standby; Take from water and be placed on the sufficient place of well-ventilated, illumination aeration 7 days, afterwards nature seawater and running water are allocated to salinity be 26PSU, finally add BZT(nitre ammonia), making its concentration is 0.3PPM, makes and supports temporarily water;
3) support temporarily the environment setting:
Perinereis aibihitensis Grube is positioned in the glass beaker that agar gel is housed prepared by step 1), and inject 100ml and support temporarily water in beaker, afterwards beaker is put into to biochemical cultivation case, connect oxygen charging pump with small size aeration stone, small size aeration stone is placed into to supporting in water temporarily of beaker upper strata, maintains in water body dissolved oxygen content all the time more than 10mg/L;
In biochemical cultivation case, temperature constant is 15 ± 1 ℃, lucifuge;
Wherein in each beaker, support temporarily 30 perinereis aibihitensis Grubes, the adult that perinereis aibihitensis Grube is 1-4cm;
4) bait feeding, water body are changed:
Change every three days and support temporarily water body, with bait feeding, synchronize and carry out; Bait is selected chlorella or Chaetoceros, changes water and the water of supporting temporarily in beaker is all outwelled before throwing something and feeding, add mix fresh algae liquid support temporarily water 100ml, the density of mixed algae liquid is 20/ml;
5) find that agar is broken serious, the timely replacing if support temporarily in process; If find that there is in temporarily foster process, health is imperfect, body surface has rotten clam worm, migrates out immediately beaker.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310360117.6A CN103430913B (en) | 2013-08-16 | 2013-08-16 | A kind of method utilizing agar to support perinereis aibihitensis Grube temporarily |
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| CN201310360117.6A CN103430913B (en) | 2013-08-16 | 2013-08-16 | A kind of method utilizing agar to support perinereis aibihitensis Grube temporarily |
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| CN103430913A true CN103430913A (en) | 2013-12-11 |
| CN103430913B CN103430913B (en) | 2016-02-03 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4800666A (en) * | 1988-02-26 | 1989-01-31 | Loren Lukehart | Method and apparatus for temporarily immobilizing an earthworm |
| CN103073113A (en) * | 2013-01-31 | 2013-05-01 | 北海市翰华生物技术有限公司 | Microorganism restoration method for water body habitat |
-
2013
- 2013-08-16 CN CN201310360117.6A patent/CN103430913B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4800666A (en) * | 1988-02-26 | 1989-01-31 | Loren Lukehart | Method and apparatus for temporarily immobilizing an earthworm |
| CN103073113A (en) * | 2013-01-31 | 2013-05-01 | 北海市翰华生物技术有限公司 | Microorganism restoration method for water body habitat |
Non-Patent Citations (4)
| Title |
|---|
| 丁理法等: "双齿围沙蚕苗种培育和生态养殖技术研究", 《水产科技情报》, vol. 32, no. 5, 31 October 2005 (2005-10-31) * |
| 张青田等: "双齿围沙蚕摄食自然沉积物的研究", 《天津科技大学学报》, vol. 23, no. 3, 30 September 2008 (2008-09-30) * |
| 李信书等: "不同水交换条件对双齿围沙蚕生长的影响", 《水产养殖》, vol. 27, no. 6, 1 November 2006 (2006-11-01) * |
| 蒋霞敏等: "双齿围沙蚕亲体培育技术试验", 《齐鲁渔业》, vol. 18, no. 4, 31 August 2001 (2001-08-31) * |
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| CN103430913B (en) | 2016-02-03 |
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Address after: 266000 Shandong city of Qingdao province Guizhou City Road No. 47 Applicant after: Shandong Marine Biology Institute Address before: 266000 Shandong city of Qingdao province Guizhou City Road No. 47 Applicant before: Shandong Province Marine Culture Institute |
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