CN103421694A - Culture medium prepared from rice and processed by-products of rice as well as application - Google Patents
Culture medium prepared from rice and processed by-products of rice as well as application Download PDFInfo
- Publication number
- CN103421694A CN103421694A CN2012104538283A CN201210453828A CN103421694A CN 103421694 A CN103421694 A CN 103421694A CN 2012104538283 A CN2012104538283 A CN 2012104538283A CN 201210453828 A CN201210453828 A CN 201210453828A CN 103421694 A CN103421694 A CN 103421694A
- Authority
- CN
- China
- Prior art keywords
- rice
- combination
- substratum
- hydrolyzed solution
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to the technical field of deep processing of foodstuffs and particularly relates to a novel culture medium which is prepared from the hydrolysates of rice/broken rice/brown rice/rice bran/the combination of rice, broken rice, brown rice and rice bran as well as other nutriments. The novel culture medium is characterized in that the hydrolysates of rice/broken rice/brown rice/rice bran/the combination of rice, broken rice, brown rice and rice bran are used as the main components of the culture medium; nutriments such as maltose, fructose, yeast powder, beef protein powder, skimmed milk, ammonium sulphate, sodium acetate, diammonium citrate, magnesium sulphate, manganese sulfate, calcium carbonate, sodium nitrate, dipotassium phosphate, potassium chloride, ferrous sulphate as well as other trace components are added into the culture medium. The culture medium provided by the invention can serve as the universal culture medium of saccharomycetes, lactic acid bacteria or mold.
Description
Technical field
The invention belongs to technical field of food deep processing, be specifically related to the substratum and the application that utilize rice and processing byproduct hydrolyzed solution thereof to prepare.The tankage that the present invention fully utilizes after rice and processing thereof prepare the special culture media that is applicable to microorganism culturing.
Background technology
The microorganism culturing such as industrial yeast culture medium carbon source commonly used is mainly sucrose, and sucrose is that raw material is made by sugarcane, and making processes comprises the pre-treatment, squeezing, the cleaning of sugarcane juice, the evaporation of sugarcane juice, the drying of boiling sugar, granulated sugar of sugarcane.In this process, produced a large amount of trade effluents, very big to environmental influence.Often also can produce a large amount of waste water in the process for preparation of existing substratum, also need to be administered simultaneously.
Be processed at rice in the process of rice and can producing about 10-15% and crack rice, the by products such as the rice bran of about 7-10%, contain the nutritive substances such as more protein, carbohydrate, abundant VITAMIN, mineral substance in these by products.At present, it is mainly to be used as feed that China utilizes the approach of rice by product, and added value is lower.It is that basis makes substratum that rice and processing byproduct hydrolyzed solution thereof are take in the present invention, makes raw material obtain utilizing fully, can be mixed with the substratum of the microorganism growth such as applicable yeast, milk-acid bacteria, mould by adding a small amount of subsidiary simultaneously.
Along with the resource scarcity occurred in world wide, comprehensive utilization to starting material processing byproduct and waste material more and more is subject to attracting attention of investigator, utilize wheat straw to be aided with W-Gum for main raw material and glucose is done carbon source production yeast culture base (Zhang Lianzhong, the research that utilizes wheat straw to prepare the yeast solid-state fermentation culture medium for main raw material. forage grass and feed, 2010(9): 93-94); The patent that publication number is CN101348768A discloses a kind of method of utilizing the beer waste water culturing yeast, adds W-Gum and makes substratum.Publication number is that CN102212480A relates to a kind of method of utilizing the tsiklomitsin dregs of a decoction to produce yeast extract, although the discarded dregs of a decoction have been carried out utilization, have reduced the pollution to environment, but dregs of a decoction treatment process trouble, make the subsidiary that substratum need add for biological fermentation more.Above-mentioned patent is utilized processing byproduct and waste material, and do microbiological culture media with the rice processing byproduct enzymolysis solution such as crack rice, there is not yet report at home and abroad.The present invention utilizes amylase, proteolytic enzyme to carry out enzymolysis to the rice processing byproduct such as crack rice and obtains the reducing sugar that content is higher, the enzymolysis solution of total free aminoacids, using the basic substance of this enzymolysis solution as substratum, add other nutritive substances according to the needs of yeast or other strain fermentations again, make the substratum of cultivating bacterial strain.
Utilization is cracked rice, and to make microbiological culture media be the new way of cracking rice and utilizing to hydrolyzed solution, and raw material availability is high, and technique is simple, practical, low in the pollution of the environment, meets the new concept of the modern utilization of resources, low-carbon environment-friendly, has vast potential for future development.
Summary of the invention
The object of the invention is to overcome the defect of prior art, a kind of utilize rice particularly to crack rice etc. substratum and application prepared by by product hydrolysis liquid is provided, with the resource circulation utilization problem after the deep processing of solution rice, find new substratum substitute for the cultivation utilization of microorganism simultaneously.
Technical scheme of the present invention be utilize rice or the processing such as crack rice after by product, be prepared into hydrolyzed solution by comprehensive utilization process, recycle the collective media that this hydrolyzed solution and the preparation of other nutritive substances are applicable to microorganism culturing.The invention still further relates to the application of this substratum in culturing micro-organisms.Realize that technology promise of the present invention is dry as described below:
The applicant has developed a kind of substratum, and its component comprises the hydrolyzed solution of rice or brown rice or rice bran or its combination, and proportioning by weight is as follows:
Rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.01-12 part;
Fructose: 0.001-4 part;
Yeast powder: 0.001-1 part;
Beef protein powder: 0.0001-1.4 part;
Skimming milk: 0.00002-1.4 part;
Ammonium sulfate: 0.00002-2.2 part;
Sodium-acetate 0.00002-1.4 part;
Dibasic ammonium citrate: 0.00002-0.8 part;
Tween 80: 0.00001-0.05 part;
Sal epsom: 0.00001-0.078 part;
Manganous sulfate: 0.00001-0.058 part;
Calcium carbonate: 0.00001-1.4 part
SODIUMNITRATE: 0.00001-1.0 part;
Dipotassium hydrogen phosphate: 0.00001-0.5 part;
Repone K: 0.00001-0.09 part;
Sulfurous acid iron: 0.00001-0.005 part;
Inositol 0.00001-0.5 part; Pantothenic acid 0.00001-0.5 part; Para-amino benzoic acid 0.00001-0.5 part; Riboflavin 0.00001-0.5 part; VitB1 0.00001-0.11 part; Vitamin H 0.00001-0.4 part; Niacin 0.00001-0.7 part; Pyridoxine hydrochloride 0.00001-0.7 part;
Described rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination prepares as follows:
(1) by material-water ratio be 1:2.5-5 water is added to rice crack rice or the hydrolyzed solution of brown rice or rice bran or its combination in, at 80-120 ℃ of lower gelatinization 20-60min, obtain the gelatinization Rice & peanut milk, be material 1;
(2) by material-water ratio, be that 1:6-9 is added to water in the material 1 of step (1), regulate the pH to 6-7 that adds the material 1 after water with the NaOH of 1mol/L, the proteolytic enzyme that adds again 1-3U/g, in 45-60 ℃ of enzyme digestion reaction 5-20min, the amylase that adds again 20-150U/g, enzyme digestion reaction 30-60min, obtain material 2;
(3) material 2 is gone out under 100 ℃ after enzyme 10-30min, under 2000-5000rpm/min, centrifugal 10-25min, get supernatant liquor, obtains material 3;
(4) 1:50-80 adds distilled water in above-mentioned material 3 by volume, and the concentration that material 3 is diluted to reducing sugar is 1-6%, is rice or cracks rice or the hydrolyzed solution of brown rice or rice bran or its combination.
Produce one of preferred version of above-mentioned substratum, component and proportioning by weight are as follows:
Rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.1-10 part;
Fructose: 0.01-3 part;
Yeast powder: 0.002-0.9 part;
Beef protein powder: 0.001-1.2 part;
Skimming milk: 0.0002-1.2 part;
Ammonium sulfate: 0.0002-2.0 part;
Sodium-acetate 0.0002-1.2 part;
Dibasic ammonium citrate: 0.0002-0.6 part;
Tween 80: 0.00002-0.03 part;
Sal epsom: 0.000015-0.068 part;
Manganous sulfate: 0.000015-0.038 part;
Calcium carbonate: 0.000015-1.1 part
SODIUMNITRATE: 0.000015-0.8 part;
Dipotassium hydrogen phosphate: 0.000015-0.3 part;
Repone K: 0.000015-0.07 part;
Sulfurous acid iron: 0.000015-0.003 part;
Inositol 0.000015-0.3 part; Pantothenic acid 0.000015-0.3 part; Para-amino benzoic acid 0.000015-0.3 part; Riboflavin 0.000015-0.3 part; VitB1 0.000015-0.09 part; Vitamin H 0.000015-0.3 part; Niacin 0.000015-0.5; Pyridoxine hydrochloride 0.000015-0.5 part.
Another preferred version of producing above-mentioned substratum is that component and proportioning by weight are as follows: rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.2-8 part; Fructose: 0.03-2 part; Yeast powder: 0.003-0.8 part; Beef protein powder: 0.002-1 part;
Skimming milk: 0.0003-1 part; Ammonium sulfate: 0.0003-1.8 part; Sodium-acetate 0.0003-1 part; Dibasic ammonium citrate: 0.0003-0.5 part; Tween 80: 0.00003-0.01 part; Sal epsom: 0.00002-0.058 part; Manganous sulfate: 0.00002-0.028 part; Calcium carbonate: 0.00002-0.9 part; SODIUMNITRATE: 0.00002-0.5 part; Dipotassium hydrogen phosphate: 0.00002-0.1 part; Repone K: 0.00002-0.05 part; Sulfurous acid iron: 0.00002-0.001 part; Inositol 0.00002-0.2 part; Pantothenic acid 0.00002-0.2 part; Para-amino benzoic acid 0.00002-0.2 part; Riboflavin 0.00002-0.2 part; VitB1 0.00002-0.08 part; Vitamin H 0.00002-0.2 part; Niacin 0.00002-0.4 part; Pyridoxine hydrochloride 0.00002-0.4 part.
The applicant provides a kind of preparation method of substratum, and the component of this substratum comprises rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination, and proportioning by weight is as follows:
Rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.01-12 part;
Fructose: 0.001-4 part;
Yeast powder: 0.001-1 part;
Beef protein powder: 0.0001-1.4 part;
Skimming milk: 0.00002-1.4 part;
Ammonium sulfate: 0.00002-2.2 part;
Sodium-acetate 0.00002-1.4 part;
Dibasic ammonium citrate: 0.00002-0.8 part;
Tween 80: 0.00001-0.05 part;
Sal epsom: 0.00001-0.078 part;
Manganous sulfate: 0.00001-0.058 part;
Calcium carbonate: 0.00001-1.4 part
SODIUMNITRATE: 0.00001-1.0 part;
Dipotassium hydrogen phosphate: 0.00001-0.5 part;
Repone K: 0.00001-0.09 part;
Sulfurous acid iron: 0.00001-0.005 part;
Inositol 0.00001-0.5 part; Pantothenic acid 0.00001-0.5 part; Para-amino benzoic acid 0.00001-0.5 part; Riboflavin 0.00001-0.5 part; VitB1 0.00001-0.11 part; Vitamin H 0.00001-0.4 part; Niacin 0.00001-0.7 part; Pyridoxine hydrochloride 0.00001-0.7 part;
Described rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination prepares as follows:
(1) by material-water ratio be 1:2.5-5 water is added to rice crack rice or the hydrolyzed solution of brown rice or rice bran or its combination in, at 80-120 ℃ of lower gelatinization 20-60min, obtain the gelatinization Rice & peanut milk, be material 1;
(2) by material-water ratio, be that 1:6-9 is added to water in the material 1 of step (1), regulate the pH to 6-7 that adds the material 1 after water with the NaOH of 1mol/L, the proteolytic enzyme that adds again 1-3U/g, in 45-60 ℃ of enzyme digestion reaction 5-20min, the amylase that adds again 20-150U/g, enzyme digestion reaction 30-60min, obtain material 2;
(3) material 2 is gone out under 100 ℃ after enzyme 10-30min, under 2000-5000rpm/min, centrifugal 10-25min, get supernatant liquor, obtains material 3;
(4) 1:50-80 adds distilled water in above-mentioned material 3 by volume, and the concentration that material 3 is diluted to reducing sugar is 1-6%, is rice or cracks rice or the hydrolyzed solution of brown rice or rice bran or its combination.
According to above-mentioned substratum preparation method's a preferred version, be that component and proportioning by weight are as follows:
Rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.1-10 part;
Fructose: 0.01-3 part;
Yeast powder: 0.002-0.9 part;
Beef protein powder: 0.001-1.2 part;
Skimming milk: 0.0002-1.2 part;
Ammonium sulfate: 0.0002-2.0 part;
Sodium-acetate 0.0002-1.2 part;
Dibasic ammonium citrate: 0.0002-0.6 part;
Tween 80: 0.00002-0.03 part;
Sal epsom: 0.000015-0.068 part;
Manganous sulfate: 0.000015-0.038 part;
Calcium carbonate: 0.000015-1.1 part
SODIUMNITRATE: 0.000015-0.8 part;
Dipotassium hydrogen phosphate: 0.000015-0.3 part;
Repone K: 0.000015-0.07 part;
Sulfurous acid iron: 0.000015-0.003 part;
Inositol 0.000015-0.3 part; Pantothenic acid 0.000015-0.3 part; Para-amino benzoic acid 0.000015-0.3 part; Riboflavin 0.000015-0.3 part; VitB1 0.000015-0.09 part; Vitamin H 0.000015-0.3 part; Niacin 0.000015-0.5; Pyridoxine hydrochloride 0.000015-0.5 part.
The preferred version of another of above-mentioned substratum preparation method is that component and proportioning by weight are as follows:
Rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.2-8 part; Fructose: 0.03-2 part; Yeast powder: 0.003-0.8 part; Beef protein powder: 0.002-1 part; Skimming milk: 0.0003-1 part; Ammonium sulfate: 0.0003-1.8 part; Sodium-acetate 0.0003-1 part; Dibasic ammonium citrate: 0.0003-0.5 part; Tween 80: 0.00003-0.01 part; Sal epsom: 0.00002-0.058 part; Manganous sulfate: 0.00002-0.028 part; Calcium carbonate: 0.00002-0.9 part; SODIUMNITRATE: 0.00002-0.5 part; Dipotassium hydrogen phosphate: 0.00002-0.1 part; Repone K: 0.00002-0.05 part; Sulfurous acid iron: 0.00002-0.001 part;
Inositol 0.00002-0.2 part; Pantothenic acid 0.00002-0.2 part; Para-amino benzoic acid 0.00002-0.2 part; Riboflavin 0.00002-0.2 part; VitB1 0.00002-0.08 part; Vitamin H 0.00002-0.2 part; Niacin 0.00002-0.4 part; Pyridoxine hydrochloride 0.00002-0.4 part.
Substratum prepared by the present invention can be applicable to be included in the cultivation of the microorganisms commonly used such as yeast, milk-acid bacteria, mould.The invention has the advantages that:
(1) utilize and crack rice hydrolyzed solution for basic culture solution, add other nutritive substance, be made into microorganism growth substratum used, greatly increased the utilization ratio of cracking rice, for rice processing has reduced cost.
(2) substratum of the present invention produces without trade effluent in making processes, environmentally safe.
(3) substratum of the present invention has suitability widely, such as the cultivation that can be applicable to the microorganisms such as yeast, milk-acid bacteria, mould.
The accompanying drawing explanation
Fig. 1: be the Yeast Growth curve.In figure: Fig. 1-a is bacterial classification CO
2Weightless situation, Fig. 1-b is that bacterial classification OD value changes.
Fig. 2: be the lactobacter growth curve.In figure: Fig. 2-a is that bacterial classification OD value changes, and Fig. 2-b is bacterium liquid pH value changing conditions.
Fig. 3: be the Aspergillus parasiticus growth curve.
Fig. 4: be Aspergillus parasiticus sporophore and spore head mirror inspection result.In figure: the sporophore of the experimental group that Fig. 4-a means and spore head,
The sporophore of the control group that Fig. 4-b means and spore head.
Embodiment
Following embodiment gives an example to the specific embodiment of the present invention with the example that is prepared as of microzyme culture medium, embodiments of the invention have the common feature that is applicable to common (such as milk-acid bacteria or mould etc.) microorganism culturing, in actual application, are not limited only to the present embodiment.
Embodiment 1: be applied to giving an example of microzyme culture medium
Preparation process of the present invention is as follows:
(1) take 30 kilograms of clean cracking rice, add the water of 80 kilograms, gelatinization 40min under boiling water bath, obtain 100 kilograms of gelatinization Rice & peanut milks, is material 1;
(2) add 180 kg of water (moisture material 1) in material 1, regulate the pH to 6-7 add material 1 after water with 1mol/LNaOH, add papoid (1.852U/g), in 55-60 ℃ of enzyme digestion reaction 5-10min, add again α-amylase (112.333U/g) enzyme digestion reaction 30-60min, obtain material 2.
(3) material 2 is gone out under 100 ℃ enzyme 10min, centrifugal 15min under 4000rpm/min, obtain 200 kilograms of materials 3;
Add 1300 kilograms of distilled water again in above-mentioned material 3, the concentration that is diluted to reducing sugar is 2-4%, is the hydrolyzed solution of cracking rice.
(4) take 15 kilograms of maltose, 15 kilograms of yeast powders (being purchased), 15 kilograms, ammonium sulfate, 15 kilograms of dipotassium hydrogen phosphates, 15 kilograms of parts of sodium-acetate, 3 kilograms of inositols, 3 kilograms, pantothenic acid, 3 kilograms of para-amino benzoic acid, 3 kilograms, riboflavin, 3 kilograms of VitB1s, join in the hydrolyzed solution of cracking rice, mix, be substratum.The above-mentioned cultivation prepared, based on sterilizing 15min under 121 ℃ of high pressure steam, had both been obtained to microzyme culture medium.
Embodiment 2: be applied to giving an example of milk-acid bacteria substratum
(1) take 30 kilograms of clean cracking rice, add the water of 80 kilograms, gelatinization 40min under boiling water bath, obtain 100 kilograms of gelatinization Rice & peanut milks, is material 1;
(2) add 180 kg of water to the moisture material 1 of material 1(to material 1), regulate the pH to 6-7 add material 1 after water with 1mol/LNaOH, add papoid (1.852U/g, be purchased), in 55-60 ℃ of enzyme digestion reaction 5-10min, add again α-amylase (112.333U/g) enzyme digestion reaction 30-60min, obtain material 2.
(3) material 2 is gone out under 100 ℃ enzyme 10min, centrifugal 15min under 4000rpm/min, obtain 200 kilograms of materials 3; Add 1300 kilograms of distilled water in above-mentioned material 3, the concentration that is diluted to reducing sugar is 2-4%, is the hydrolyzed solution of cracking rice.
(4) take 10 kilograms of maltose, 15 kilograms of yeast powders (being purchased), 15 kilograms, beef protein powder, 15 kilograms of skimming milks, 15 kilograms of sodium-acetates, 7.5 kilograms of dibasic ammonium citrates, 0.15 kilogram of tween 80,0.87 kilogram, sal epsom, 0.42 kilogram of manganous sulfate, join in the hydrolyzed solution of cracking rice, mix, be substratum.The above-mentioned cultivation prepared, based on sterilizing 15min under 121 ℃ of high pressure steam, is obtained to the milk-acid bacteria substratum.
Embodiment 3: be applied to giving an example of mould medium
(1) take 30 kilograms of clean cracking rice, add the water of 80 kilograms, gelatinization 40min under boiling water bath, obtain 100 kilograms of gelatinization Rice & peanut milks, is material 1;
(2) add 180 kg of water to material 1 to material 1, the material 1 that 1(is moisture), regulate the pH to 6-7 add material 1 after water with 1mol/LNaOH, add papoid (1.852U/g), in 55-60 ℃ of enzyme digestion reaction 5-10min, add again α-amylase (112.333U/g) enzyme digestion reaction 30-60min, obtain material 2.
(3) material 2 is gone out under 100 ℃ enzyme 10min, centrifugal 15min under 4000rpm/min, obtain 200 kilograms of materials 3; Add 1300 kilograms of distilled water in above-mentioned material 3, the concentration that is diluted to reducing sugar is 2-4%, is the hydrolyzed solution of cracking rice.
(4) take 15 kilograms of maltose, 0.015 kilogram, sal epsom, 7.5 kilograms of SODIUMNITRATE, 1.5 kilograms of dipotassium hydrogen phosphates, 0.75 kilogram, Repone K, 0.015 kilogram of sulfurous acid iron, join in the hydrolyzed solution of cracking rice, and mixes, and is substratum.The above-mentioned cultivation prepared, based on sterilizing 15min under 121 ℃ of high pressure steam, is obtained to mould medium.
Embodiment 4: the test of substratum of the present invention on the impact of Yeast Growth characteristic
The microzyme culture medium that test adopts embodiment 1 to make.The present embodiment detects the characteristic that the yeast of this culture medium culturing cultivates and the fermented rice cake organoleptic quality is had or not to impact.The microzyme culture medium that test group adopts embodiment 1 method to make, control group is yeast substratum (YPD) commonly used: 1% yeast extract paste, 2% peptone, 2% glucose.Select yeast Hua Zhong Agriculture University authorized Karst British mold (Brettanomyces custersii) the ZSM-001(patent No. of cultivating: 2007100536112, patent publication No. is: CN101173223; The preserving number at Chinese Typical Representative culture collection center is CCTCCNO:M207150).This yeast is cultivated based on 30 ℃ of cultivations with above-mentioned two groups respectively, and timing sampling, regularly weigh, and records weightless situation, and measure the OD of fermented liquid
600Value.It the results are shown in Figure 1.
According to document (Xiong Qing, model reveals, Bao Fangfang, Zhao Siming etc. fermented rice cake flavor formation and feature, Food science, 2011,32 (24): the method for making fermented rice cake 232-236) is made fermented rice cake.Adopt five people's point systems, the sensory test of fermented rice cake mainly analyzed from several eating quality indexs such as color and luster, form, flavour, fragrance, mouthfeels.Fluffy with the fermented rice cake form, color and luster is pure white evenly has soft fermented flavour and alcohol smell, and sour-sweet moderate, mouthfeel is soft is best result (full marks 10 minutes), and total points is indices score sum.Its results of sensory evaluation is in Table 1.The fermented rice cake that wherein test group is made for the yeast of the culture medium culturing of cracking rice according to the making of present patent application introduction method, the fermented rice cake that control group is made of the yeast of YPD culture medium culturing.
The impact of the yeast that table 1 different culture media is cultivated on the fermented rice cake organoleptic quality
As shown in Figure 1, in two groups of substratum, the equal well-grown of yeast, the yeast of test group is in the increment of logarithmic phase a little more than control group, and both growth cycle time is substantially close, and finally in the OD of stationary phase value, tends towards stability at both.As can be seen from Table 1, above-mentioned test group and control group to fermented rice cake aspect organoleptic quality without larger difference, illustrate that substratum of the present invention does not have much affect to saccharomycetic tunning, utilize this substratum can meet in the Yeast Growth process demand to nutritive substance fully.
Embodiment 5: the test of substratum of the present invention on the impact of lactobacter growth characteristic
Adopt embodiment 2 to make the substratum that milk-acid bacteria is cultivated.The present embodiment detects the impact of this culture medium culturing on the lactobacter growth characteristic.The milk-acid bacteria substratum that test group adopts embodiment 2 methods to make, control group is MRS substratum (1% beef protein powder, 1% flesh of fish juice, 0.5% Yeast diffusion juice powder, 2% glucose, 0.5% sodium-acetate, 0.2% dibasic ammonium citrate, 0.01% tween 80,0.058% sal epsom, 0.028% manganous sulfate, with pressure kettle at 121 ℃ of sterilizing 15min, regulate pH6.2～6.4), the authorized milk-acid bacteria of the Hua Zhong Agriculture University of employing is plant lactobacillus (Lactobacillus plantarum) ZSM-002, the patent No.: 2009100638581; The preserving number at Chinese Typical Representative culture collection center is CCTCC NO:M209127.This milk-acid bacteria is cultivated based on 30 ℃ of cultivations respectively to timing sampling, pH value and the OD of mensuration fermented liquid with above-mentioned two groups
600Value.Get the fermented liquid of 40h and measure its acidity.It the results are shown in Figure 2 and table 2.
Wherein acid test adopts the NaOH volumetry, by State Standard of the People's Republic of China GB5409-1989 method, measures, and acidity means with mmol/100g.
The impact of table 2 different culture media confrontation fermented liquid acidity (40h)
As shown in Figure 2, in two groups of substratum, the equal well-grown of milk-acid bacteria, the milk-acid bacteria of test group in the increment of logarithmic phase a little more than control group, both growth cycle time is substantially close, and finally in the pH of stationary phase value, tends towards stability at both, in the 3.92-4.03 left and right.As shown in Table 2, both final acid producing abilities are roughly the same, are distributed in the 4.4-4.5mmol/100g scope.From Fig. 2, table 2, the milk-acid bacteria substratum that adopts this technological invention has promoter action for the propagation of milk-acid bacteria, also can guarantee the nutritional needs of the product acid of milk-acid bacteria simultaneously.
Embodiment 6: the test of substratum of the present invention on the impact of mould bacteria biomass
Adopt embodiment 3 to make the substratum of mycotic culture.The present embodiment detects the impact of this culture medium culturing on the mould-growth characteristic.The mould medium that test group adopts embodiment 3 methods to make, control group is potato glucose substratum (formula: potato 20g, glucose 2g, water 100ml.Take peeling potato 20g, be cut into small pieces, add 100mL water, boil 1h, with double gauze, be filtered into clear liquid, add glucose 2g, and supplement the moisture reduced because of evaporation).The mould that the present embodiment adopts is Aspergillus parasiticus (Aspergillus parasiticus), and this bacterial strain is preserved in Chinese industrial microbial strains preservation administrative center, and preserving number is that CICC40037(is purchased from Chinese industrial microbial strains preservation administrative center), (
Http:// www.china-cicc.org/searchresult.aspx bianhao=40037& Zhongming=& Shuming=& Fenli=& Zhongwenming=& Qitabianhao=& Yongtu=& Wendu=& Laiyuan=& Peiyangji=0& Jiage=& T=1).
Above-mentioned Aspergillus parasiticus CICC40037 is cultivated based on 28 ℃ of cultivations with above-mentioned two groups respectively, and 28 ℃, 180r/min, cultivate 48h.Timing sampling, regularly weigh, and measures its strain bio amount.The biometric measurement metering method is mycotic culture liquid vacuum filtration 5min, and the sterilized water washing is collected thalline three times.Get the thalline of above-mentioned collection, 105 ℃ dry to constant weight and obtain dry cell weight.It the results are shown in Figure 3.Compare two groups of hypogenous moulds of substratum, its form as shown in Figure 4.
As shown in Figure 3, in two groups of substratum, the equal well-grown of Aspergillus parasiticus, the Aspergillus parasiticus of test group (the present invention) is in the increment of logarithmic phase a little more than control group, and both growth cycle time is substantially close.And as shown in Figure 4, the mould form of growing under two kinds of substratum is without larger difference.The colony diameter of 2-8 days is 1.5-10cm, and colonial morphology is comprised of thicker matrix mycelia.Bacterium colony has radial rill.It is green that the bacterium colony surface is, and color is darker, and reverse side is Vandyke brown or cream color.Mycelia is bordering on smooth, or slightly coarse.Divide and bear sporophore from vegetative hyphae, sporophore is shorter, and there is indivedual bacterial strains long spore hardly at edge, and white, be the ulotrichy radiation.Conidiophore is born in matrix, shorter, and conidium is chain and life, and the bright orange green of conidium is radiation, loose, and there is the thorn-like projection at edge.Therefore show, the substratum that adopts the hydrolyzed solution of cracking rice of the present invention to make is the growth that can promote preferably mould.
Embodiment 7: the substratum that several rice processing byproducts (combination of raw material) are made for example
Preparation process of the present invention is as follows:
(1) take 20 kilograms of clean cracking rice, 6 kilograms of brown rice after washing, 4 kilograms of rice brans after washing, add the water of 80 kilograms, and gelatinization 40min under boiling water bath, obtain 100 kilograms of gelatinization Rice & peanut milks, is material 1;
(2) add 180 kg of water (moisture material 1) in material 1, regulate the pH to 6-7 add material 1 after water with 1mol/LNaOH, add papoid (1.852U/g), in 55-60 ℃ of enzyme digestion reaction 5-10min, add again α-amylase (112.333U/g) enzyme digestion reaction 30-60min, obtain material 2.
(3) material 2 is gone out under 100 ℃ enzyme 10min, centrifugal 15min under 4000rpm/min, obtain 200 kilograms of materials 3; Add 1300 kilograms of distilled water again in above-mentioned material 3, the concentration that is diluted to reducing sugar is 2-4%, is the hydrolyzed solution of cracking rice.
(4) take 15 kilograms of maltose, 15 kilograms of yeast powders (being purchased), 15 kilograms, ammonium sulfate, 15 kilograms of dipotassium hydrogen phosphates, 15 kilograms of parts of sodium-acetate, 3 kilograms of inositols, 3 kilograms, pantothenic acid, 3 kilograms of para-amino benzoic acid, 3 kilograms, riboflavin, 3 kilograms of VitB1s, join in the hydrolyzed solution of cracking rice, mix, be substratum.The above-mentioned cultivation prepared, based on sterilizing 15min under 121 ℃ of high pressure steam, had both been obtained to microzyme culture medium.
Claims (7)
1. a substratum, is characterized in that, the component of this substratum comprises rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination, and proportioning by weight is as follows:
Rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.01-12 part;
Fructose: 0.001-4 part;
Yeast powder: 0.001-1 part;
Beef protein powder: 0.0001-1.4 part;
Skimming milk: 0.00002-1.4 part;
Ammonium sulfate: 0.00002-2.2 part;
Sodium-acetate 0.00002-1.4 part;
Dibasic ammonium citrate: 0.00002-0.8 part;
Tween 80: 0.00001-0.05 part;
Sal epsom: 0.00001-0.078 part;
Manganous sulfate: 0.00001-0.058 part;
Calcium carbonate: 0.00001-1.4 part;
SODIUMNITRATE: 0.00001-1.0 part;
Dipotassium hydrogen phosphate: 0.00001-0.5 part;
Repone K: 0.00001-0.09 part;
Sulfurous acid iron: 0.00001-0.005 part;
Inositol 0.00001-0.5 part; Pantothenic acid 0.00001-0.5 part; Para-amino benzoic acid 0.00001-0.5 part; Riboflavin 0.00001-0.5 part; VitB1 0.00001-0.11 part; Vitamin H 0.00001-0.4 part; Niacin 0.00001-0.7 part; Pyridoxine hydrochloride 0.00001-0.7 part;
Wherein:
Described hydrolyzed solution prepares as follows:
(1) be that 1:2.5-5 is added to rice by water or cracks rice or brown rice or rice bran or its combination by material-water ratio, at 80-120 ℃ of lower gelatinization 20-60min, obtain the gelatinization Rice & peanut milk, be material 1;
(2) by material-water ratio, be that 1:6-9 is added to water in the material 1 of step (1), regulate the pH to 6-7 that adds the material 1 after water with the NaOH of 1mol/L, the proteolytic enzyme that adds again 1-3U/g, in 45-60 ℃ of enzyme digestion reaction 5-20min, the amylase that adds again 20-150U/g, enzyme digestion reaction 30-60min, obtain material 2;
(3) material 2 is gone out under 100 ℃ after enzyme 10-30min, under 2000-5000rpm/min, centrifugal 10-25min, get supernatant liquor, obtains material 3;
(4) 1:50-80 adds distilled water in above-mentioned material 3 by volume, and the concentration that material 3 is diluted to reducing sugar is 1-6%, is rice or cracks rice or the hydrolyzed solution of brown rice or rice bran or its combination.
2. substratum as claimed in claim 1, is characterized in that, component and proportioning by weight are as follows:
Rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.1-10 part;
Fructose: 0.01-3 part;
Yeast powder: 0.002-0.9 part;
Beef protein powder: 0.001-1.2 part;
Skimming milk: 0.0002-1.2 part;
Ammonium sulfate: 0.0002-2.0 part;
Sodium-acetate 0.0002-1.2 part;
Dibasic ammonium citrate: 0.0002-0.6 part;
Tween 80: 0.00002-0.03 part;
Sal epsom: 0.000015-0.068 part;
Manganous sulfate: 0.000015-0.038 part;
Calcium carbonate: 0.000015-1.1 part
SODIUMNITRATE: 0.000015-0.8 part;
Dipotassium hydrogen phosphate: 0.000015-0.3 part;
Repone K: 0.000015-0.07 part;
Sulfurous acid iron: 0.000015-0.003 part;
Inositol 0.000015-0.3 part; Pantothenic acid 0.000015-0.3 part; Para-amino benzoic acid 0.000015-0.3 part; Riboflavin 0.000015-0.3 part; VitB1 0.000015-0.09 part; Vitamin H 0.000015-0.3 part; Niacin 0.000015-0.5; Pyridoxine hydrochloride 0.000015-0.5 part.
3. substratum as claimed in claim 1 or 2 is characterized in that component proportioning by weight wherein is as follows:
Rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.2-8 part; Fructose: 0.03-2 part; Yeast powder: 0.003-0.8 part; Beef protein powder: 0.002-1 part; Skimming milk: 0.0003-1 part; Ammonium sulfate: 0.0003-1.8 part; Sodium-acetate 0.0003-1 part; Dibasic ammonium citrate: 0.0003-0.5 part; Tween 80: 0.00003-0.01 part; Sal epsom: 0.00002-0.058 part; Manganous sulfate: 0.00002-0.028 part; Calcium carbonate: 0.00002-0.9 part; SODIUMNITRATE: 0.00002-0.5 part; Dipotassium hydrogen phosphate: 0.00002-0.1 part; Repone K: 0.00002-0.05 part; Sulfurous acid iron: 0.00002-0.001 part; Inositol 0.00002-0.2 part; Pantothenic acid 0.00002-0.2 part; Para-amino benzoic acid 0.00002-0.2 part; Riboflavin 0.00002-0.2 part; VitB1 0.00002-0.08 part; Vitamin H 0.00002-0.2 part; Niacin 0.00002-0.4 part; Pyridoxine hydrochloride 0.00002-0.4 part.
4. the preparation method of a substratum, is characterized in that, the component of this substratum comprises rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination, and proportioning by weight is as follows:
Rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.01-12 part;
Fructose: 0.001-4 part;
Yeast powder: 0.001-1 part;
Beef protein powder: 0.0001-1.4 part;
Skimming milk: 0.00002-1.4 part;
Ammonium sulfate: 0.00002-2.2 part;
Sodium-acetate 0.00002-1.4 part;
Dibasic ammonium citrate: 0.00002-0.8 part;
Tween 80: 0.00001-0.05 part;
Sal epsom: 0.00001-0.078 part;
Manganous sulfate: 0.00001-0.058 part;
Calcium carbonate: 0.00001-1.4 part
SODIUMNITRATE: 0.00001-1.0 part;
Dipotassium hydrogen phosphate: 0.00001-0.5 part;
Repone K: 0.00001-0.09 part;
Sulfurous acid iron: 0.00001-0.005 part;
Inositol 0.00001-0.5 part; Pantothenic acid 0.00001-0.5 part; Para-amino benzoic acid 0.00001-0.5 part; Riboflavin 0.00001-0.5 part; VitB1 0.00001-0.11 part; Vitamin H 0.00001-0.4 part; Niacin 0.00001-0.7 part; Pyridoxine hydrochloride 0.00001-0.7 part;
Described rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination prepares as follows:
(1) by material-water ratio be 1:2.5-5 water is added to rice crack rice or the hydrolyzed solution of brown rice or rice bran or its combination in, at 80-120 ℃ of lower gelatinization 20-60min, obtain the gelatinization Rice & peanut milk, be material 1;
(2) by material-water ratio, be that 1:6-9 is added to water in the material 1 of step (1), regulate the pH to 6-7 that adds the material 1 after water with the NaOH of 1mol/L, the proteolytic enzyme that adds again 1-3U/g, in 45-60 ℃ of enzyme digestion reaction 5-20min, the amylase that adds again 20-150U/g, enzyme digestion reaction 30-60min, obtain material 2;
(3) material 2 is gone out under 100 ℃ after enzyme 10-30min, under 2000-5000rpm/min, centrifugal 10-25min, get supernatant liquor, obtains material 3;
(4) 1:50-80 adds distilled water in above-mentioned material 3 by volume, and the concentration that material 3 is diluted to reducing sugar is 1-6%, is rice or cracks rice or the hydrolyzed solution of brown rice or rice bran or its combination.
5. the preparation method of substratum as claimed in claim 4, is characterized in that, proportioning by weight is as follows:
Rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.1-10 part;
Fructose: 0.01-3 part;
Yeast powder: 0.002-0.9 part;
Beef protein powder: 0.001-1.2 part;
Skimming milk: 0.0002-1.2 part;
Ammonium sulfate: 0.0002-2.0 part;
Sodium-acetate 0.0002-1.2 part;
Dibasic ammonium citrate: 0.0002-0.6 part;
Tween 80: 0.00002-0.03 part;
Sal epsom: 0.000015-0.068 part;
Manganous sulfate: 0.000015-0.038 part;
Calcium carbonate: 0.000015-1.1 part
SODIUMNITRATE: 0.000015-0.8 part;
Dipotassium hydrogen phosphate: 0.000015-0.3 part;
Repone K: 0.000015-0.07 part;
Sulfurous acid iron: 0.000015-0.003 part;
Inositol 0.000015-0.3 part; Pantothenic acid 0.000015-0.3 part; Para-amino benzoic acid 0.000015-0.3 part; Riboflavin 0.000015-0.3 part; VitB1 0.000015-0.09 part; Vitamin H 0.000015-0.3 part; Niacin 0.000015-0.5; Pyridoxine hydrochloride 0.000015-0.5 part.
6. as the preparation method of claim 4 or 5 described substratum, it is characterized in that component proportioning by weight wherein is as follows: rice or crack rice or the hydrolyzed solution of brown rice or rice bran or its combination: 100 parts;
Maltose: 0.2-8 part; Fructose: 0.03-2 part; Yeast powder: 0.003-0.8 part; Beef protein powder: 0.002-1 part; Skimming milk: 0.0003-1 part; Ammonium sulfate: 0.0003-1.8 part; Sodium-acetate 0.0003-1 part; Dibasic ammonium citrate: 0.0003-0.5 part; Tween 80: 0.00003-0.01 part; Sal epsom: 0.00002-0.058 part; Manganous sulfate: 0.00002-0.028 part; Calcium carbonate: 0.00002-0.9 part; SODIUMNITRATE: 0.00002-0.5 part; Dipotassium hydrogen phosphate: 0.00002-0.1 part; Repone K: 0.00002-0.05 part; Sulfurous acid iron: 0.00002-0.001 part; Inositol 0.00002-0.2 part; Pantothenic acid 0.00002-0.2 part; Para-amino benzoic acid 0.00002-0.2 part; Riboflavin 0.00002-0.2 part; VitB1 0.00002-0.08 part; Vitamin H 0.00002-0.2 part; Niacin 0.00002-0.4 part; Pyridoxine hydrochloride 0.00002-0.4 part.
7. the application of the described substratum of claim 1-3 any one in yeast, milk-acid bacteria or mycotic culture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210453828.3A CN103421694B (en) | 2012-11-13 | 2012-11-13 | Culture medium prepared from rice and processed by-products of rice as well as application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210453828.3A CN103421694B (en) | 2012-11-13 | 2012-11-13 | Culture medium prepared from rice and processed by-products of rice as well as application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103421694A true CN103421694A (en) | 2013-12-04 |
| CN103421694B CN103421694B (en) | 2015-01-28 |
Family
ID=49647089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210453828.3A Expired - Fee Related CN103421694B (en) | 2012-11-13 | 2012-11-13 | Culture medium prepared from rice and processed by-products of rice as well as application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103421694B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101407754A (en) * | 2007-10-11 | 2009-04-15 | 朴美子 | Production process of black fungus rough rice vinegar |
| CN101914602A (en) * | 2010-08-11 | 2010-12-15 | 黑龙江八一农垦大学 | Natamycin fermentation medium |
| CN101979616A (en) * | 2010-11-12 | 2011-02-23 | 合肥工业大学 | Method for producing erythritol by using broken rice |
| US20120245322A1 (en) * | 2011-03-25 | 2012-09-27 | Hyundai Motor Company | Manufacturing lactide from lactic acid |
-
2012
- 2012-11-13 CN CN201210453828.3A patent/CN103421694B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101407754A (en) * | 2007-10-11 | 2009-04-15 | 朴美子 | Production process of black fungus rough rice vinegar |
| CN101914602A (en) * | 2010-08-11 | 2010-12-15 | 黑龙江八一农垦大学 | Natamycin fermentation medium |
| CN101979616A (en) * | 2010-11-12 | 2011-02-23 | 合肥工业大学 | Method for producing erythritol by using broken rice |
| US20120245322A1 (en) * | 2011-03-25 | 2012-09-27 | Hyundai Motor Company | Manufacturing lactide from lactic acid |
Non-Patent Citations (5)
| Title |
|---|
| DIETER WILD等: "New Monascus Metabolites with a Pyridine Structure in Red Fermented Rice", 《J.AGRIC.FOOD CHEM.》 * |
| 乔凤云 等: "利用稻米及其副产品生产功能食品和高蛋白饲料", 《中国稻米》 * |
| 王剑锋 等: "花脸香蘑液态发酵条件研究", 《中国食用菌》 * |
| 谭军 等: "培养基原料对酵母转化硒的影响研究", 《微量元素与健康研究》 * |
| 陈志杰 等: "以糙米为主要原料的灵芝深层发酵培养基优化", 《中国食品学报》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103421694B (en) | 2015-01-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105039453A (en) | Method for preparing rice bran polysaccharides with improved oxidation resistance and application of rice bran polysaccharides | |
| CN102618587B (en) | Method for producing acetoin by vinasse fermentation and producing tetramethylpyrazine by acetoin transformation | |
| CN103602590A (en) | Method of preparing functional monascus mycelia and fermentation liquor by liquid fermentation, and products of the monascus mycelia and the fermentation liquor | |
| CN110916177B (en) | Method for preparing kelp enzyme by enzyme fermentation coupling technology | |
| CN103540553A (en) | Method for mixing and fermenting compound strain to prepare mare milk vinegar and prepared mare milk vinegar | |
| CN104195051A (en) | Preparation method and product of functional red pitaya yeast | |
| CN111057637B (en) | Multi-strain fermented apple vinegar rich in free amino acids and production process thereof | |
| CN104480150A (en) | Biological enrichment method of conjugated linolenic acid isomer | |
| CN102987063B (en) | Organic acid animal growth regulator and preparation method thereof | |
| CN102337225B (en) | Preparation method of high-nitrogen fresh yeast and extract | |
| CN104651109B (en) | A kind of fermentation process of banana flavor medicated beer | |
| CN105802881B (en) | Method for improving biomass and acid yield of lactic acid bacteria | |
| KR101252132B1 (en) | Feed additives for producing milk having high amount of milk proteins and process for the preparation of feed for lactating dairy cattle using the same | |
| CN101570745A (en) | Technology for preparing lactose enzyme | |
| CN103392920A (en) | Fermentation method of soybean hulls | |
| WO2014098277A1 (en) | Method for preparing seasoning material from lees by continuous processs of enzyme decomposition and lactobacillus fermentation | |
| CN110583854A (en) | High-acid-soluble protein and high-lactic acid fermented soybean meal and preparation method thereof | |
| CN106035985A (en) | Method for producing single cell proteins by using processed waste from mixed bacteria liquid fermentation of yellow wine | |
| CN104711208B (en) | A kind of lactic acid bacteria with high starch capacity of decomposition | |
| CN103421694B (en) | Culture medium prepared from rice and processed by-products of rice as well as application | |
| CN101717789B (en) | Method for preparing culture medium for efficiently producing haematochrome | |
| CN103919222A (en) | Preparation method of pholiota adipose composite health-care beverage | |
| CN110973354B (en) | Solid-state fermentation wheat bran feed and preparation method and application thereof | |
| CN111019839A (en) | Method for degrading herbal tea residues and producing probiotics by combined fermentation of aspergillus niger and saccharomycetes | |
| CN104651157A (en) | Production method of yak whey low alcohol beverage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150128 Termination date: 20201113 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |