CN103416585A - Method for improving boiler chicken production performance and chicken quality by microecologics - Google Patents
Method for improving boiler chicken production performance and chicken quality by microecologics Download PDFInfo
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Abstract
The invention discloses a method for improving boiler chicken production performance and chicken quality by microecologics. The method includes the step that chicken quality of boiler chicken fed by chicken daily ration containing the microecologics is better than that of boiler chicken fed by ordinary chicken daily ration. Active ingredients of the microecologics are cultures obtained by using fermentation culture medium to cultivate complex microbial inoculants; active ingredients of the complex microbial inoculants are composed of bacillus subtilis, saccharomyces cerevisiae and lactobacillus; the chicken daily ration containing the microecologics is prepared by adding 0.015% to 0.05% of the microecologics in mass to the ordinary chicken daily ration, and the ordinary chicken daily ration is free of the microecologics.
Description
Technical field
The present invention relates to a kind of method that improves meat chicken production performance and chicken meat quality, particularly a kind of broiler chicken that utilizes improves the method for meat chicken production performance and chicken meat quality with the probiotics preparation.
Background technology
Broiler chicken is in the long-term evolution process, microorganism and host in alimentary canal have formed stable symbiosis, can keep the microecological balance in Broiler Chicken, there is the Development of Digestive Organs of promotion, resist the functions such as pathogenic microorganisms is encroached on, buffering unfavorable factor burst impact, be conducive to broiler chicken and keep the good general level of the health and production performance.In recent years, the broiler breeding scale enlarges gradually, although industrialized cultivation scale has been brought larger economic benefit for the raiser, but the unfavorable factor such as popular of the prophylactic agents such as the breeding environment that intensive farm is followed deterioration, antibiotic abuses, epidemic disease has affected the microecological balance in Broiler Chicken greatly, gut development, physiological metabolism, anti-stress ability that causes broiler chicken etc. remains at low levels, and then causing the production performance of broiler chicken to descend, genetic potential can't be given full play to.Along with the develop rapidly of modern broiler breeding industry, feeding of broiler planningization, intensive process are accelerated day by day, favourable guarantee the consumption demand of domestic market to chicken meat product.But improving constantly of people's living standard, have higher requirement to chicken meat quality in recent years.At present, growth of meat chicken speed is with the speed increase of annual 1.0%-1.5%, and the trunk overfertilization appears in broiler chicken, and carnification obtains slightly always, loosens, lacks local flavor.Dissect the factor that the eaten by broilers quality descends and show, broiler chicken is thicker than the crude fibre of laying hen; In meat, the kind of flavour thing and flavor precursor and content can reflect the Meat Flavor characteristic to a certain extent, and what the solvents such as moisture, protein, fat can affect various flavor substances is the flavor effect; Intramuscular fat content has considerable influence to the tender degree of poultry, succulence etc.Thereby, explore safe and effective technological means, improving the production performance of broiler chicken and the quality of chicken meat product is broiler breeding important technological problems urgently to be resolved hurrily.
Probiotics is green, safe additive, drug residue free, does not cause environmental hazard, to Product quality and safety and environment for human survival, can not cause reality and potentiality to threaten, and is one of developing direction of safe feed additive.
Summary of the invention
A technical problem to be solved by this invention is to provide a kind of method that improves chicken meat and production performance.
The method of raising chicken meat provided by the present invention and production performance, comprise with the daily ration of broiler feeding dorking that contains probiotics and obtain production performance and chicken meat quality all higher than the step of the broiler chicken with the common Diet of broiler chicken, the active component of described probiotics is to cultivate with fermentation medium the culture that composite bacteria agent capable obtains; The active component of described composite bacteria agent capable is comprised of bacillus subtilis, saccharomyces cerevisiae and Lactobacillus plantarum; The described daily ration of broiler that contains probiotics is to be the feed that the described probiotics of 0.015%-0.05% of the common daily ration quality of described broiler chicken obtains to adding in the common daily ration of described broiler chicken, in the common daily ration of described broiler chicken, does not contain described probiotics.
In the method, the method raised table hens is except the daily ration difference, and other condition (comprising layer breed, feeding environment, feeding method) is all identical.
In said method, described bacillus subtilis can be bacillus subtilis RJGP16, described saccharomyces cerevisiae can be saccharomyces cerevisiae CICC1562 and described Lactobacillus plantarum can be Lactobacillus plantarum CICC22696.
In said method, in the active component of described composite bacteria agent capable, the colony forming unit number ratio of described bacillus subtilis, described saccharomyces cerevisiae and described Lactobacillus plantarum can be 1:1-3:1 (as 1:3:1 or 1:1:1).
In said method, described raising chicken meat is following A 1)-A15) in all or part of:
A1) improve the valine content of broiler chicken chicken;
A2) improve the methionine content of broiler chicken chicken;
A3) improve the lysine content of broiler chicken chicken;
A4) improve the phenylalanine content of broiler chicken chicken;
A5) improve the cystine of broiler chicken chicken;
A6) improve the tyrosine content of broiler chicken chicken;
A7) improve the proline content of broiler chicken chicken;
A8) improve the crude protein content of broiler chicken chicken;
A9) reduce the cholesterol level of broiler chicken chicken;
A10) reduce the fat content of broiler chicken chicken;
A11) reduce the drip loss of broiler chicken chicken;
A12) improve the tender degree of chest muscle of broiler chicken;
A13) improve the chest muscle elasticity of broiler chicken;
A14) improve the chewiness of chicken leg muscle;
A15) improve the chewiness of chicken chest muscle meat;
Described production performance is following B1)-B6) in whole or five kinds or four kinds or three kinds or two kinds or a kind of:
B1) rate of animals delivered to the slaughter-house;
B2) meat feed ratio;
B3) apparent digestibility of protein;
B4) apparent digestibility of calcium;
B5) apparent digestibility of phosphorus;
B6) body weight.
In said method, every liter of described fermentation medium can be prepared as follows: every liter of described fermentation medium can be prepared as follows: 10g carbon source, 10g nitrogenous source, 1.5g KH
2PO
4, 0.80g MnSO
4, 1.00g MgSO
4, 1.0g NaCl, water is settled to 1000mL, pH value 6.0-7.0 (as 6.5); Described carbon source is glucose or sucrose; Described nitrogenous source is peptone or dusty yeast.
In said method, described composite bacteria agent capable can be the liquid spawn of the liquid spawn of the liquid spawn of described bacillus subtilis, described saccharomyces cerevisiae and described Lactobacillus plantarum according to the colony forming unit number of 1:1-3:1 (as 1:3:1 or 1:1:1) than the mixture be mixed to get.
In said method, the liquid spawn of described bacillus subtilis can be with described fermentation medium and cultivates at 30-32 ℃ the culture (all substances in culture vessel) that 18-24h obtains;
The liquid spawn of described saccharomyces cerevisiae can be with described fermentation medium and cultivates at 28-30 ℃ the culture (all substances in culture vessel) that 32-40h (as 36 hours) obtains;
The liquid spawn of described Lactobacillus plantarum can be with described fermentation medium and cultivates at 35-37 ℃ the culture (all substances in culture vessel) that 46-54h (as 48 hours) obtains.
In said method, the described condition of culture adopted with fermentation medium cultivation composite bacteria agent capable can be: cultivation temperature 34-36 ℃, incubation time 42-48 hour, the inoculum concentration of described composite bacteria agent capable (volume of the described composite bacteria agent capable of access and the volume ratio of described fermentation medium) is 6% (volume ratio).
In said method, the described daily ration of broiler that contains probiotics specifically can be a) or b) or c):
A) to adding in the common daily ration of described broiler chicken, be the feed that 0.02% described probiotics of the common daily ration quality of described broiler chicken obtains;
B) to adding in the common daily ration of described broiler chicken, be the feed that 0.025% described probiotics of the common daily ration quality of described broiler chicken obtains;
C) to adding in the common daily ration of described broiler chicken, be the feed that the described probiotics of 0.02%-0.025% of the common daily ration quality of described broiler chicken obtains.
In said method, the common daily ration of described broiler chicken can be the common daily ration of broiler chicken brood time and/or the common daily ration of broiler chicken finishing period, correspondingly, the described daily ration of broiler that contains probiotics divides the broiler chicken finishing period that can be the broiler chicken brood time daily ration that contains probiotics and/or contain probiotics daily ration;
The described broiler chicken brood time daily ration that contains probiotics is to be the feed that the described probiotics of 0.015%-0.05% of the common daily ration quality of described broiler chicken brood time obtains to adding in the common daily ration of described broiler chicken brood time, in the common daily ration of described broiler chicken brood time, does not contain described probiotics;
The described broiler chicken finishing period daily ration that contains probiotics is to be the feed that the described probiotics of 0.015%-0.05% of the common daily ration quality of described broiler chicken finishing period obtains to adding in the common daily ration of described broiler chicken finishing period, in the common daily ration of described broiler chicken finishing period, does not contain described probiotics.
In the described broiler chicken brood time daily ration that contains probiotics and the described broiler chicken finishing period daily ration that contains probiotics, the mass content of described probiotics all can be 0.02%-0.025%, 0.02% or 0.025%.
Above, described probiotics can be only be comprised of the active component of described probiotics.Described probiotics also can be comprised of active component and the carrier of described probiotics; Described carrier specifically can be at least one in calcium phosphate, powdered rice hulls, wheat bran, rice bran, skimmed milk power, maltodextrin, sucrose, glycerine and starch.
Above, the thalline content of described probiotics can be 5 * 10
8-8 * 10
9Cfu/g, as 6 * 10
9Cfu/g.
Above, described brood time can be 1-the 28th age in days, and described finishing period can be the 29th age in days-deliver for sale, as 29th age in days-the 42nd age in days, 29th age in days-the 46th age in days, 29th age in days-the 46th age in days.
Above, the daily ration of broiler feeding dorking that described use contains probiotics is the feed broiler chicken of 1 age in days of the daily ration of broiler that contains probiotics by described use.
In an embodiment of the invention, described broiler chicken is for liking to pull out broiler chicken or Ai Wei mattress broiler chicken increasingly.
The common daily ration of described broiler chicken can contain energy feed, protein feeds, mineral feed and vitamin feed.
Described energy feed can be energy feed commonly used, and as be selected from least one in following substances: paddy reality, bran, potato, grass-seed and tree are real etc.Described protein feeds can be protein feeds commonly used, as be selected from least one in following substances: Soybean Meal, Cottonseed Meal, corn germ cake, meat meal tankage, rapeseed dregs, peanut meal and the certain herbaceous plants with big flowers dregs of rice etc.
Described mineral feed can be selected from stone flour, calcium bicarbonate, salt, oyster shell whiting, bone meal, calcium monohydrogen phosphate, calcium carbonate, salt, copper sulphate, ferrous sulfate, manganese sulfate, KI, zinc oxide, sodium selenite and cobalt chloride etc.
Described vitamin feed can be vitamin feed commonly used, as be selected from least one in following substances: vitamin A, cholecalciferol, vitamin E, prokeyvit, vitamin B1 g, vitamin B2, vitamin B6, cobalamin, nicotinic acid, D-VB5 calcium, biotin, the described antioxidant of Choline Chloride can be vitamin E (alpha-tocopherol) or butylated hydroxy anisole (BHA).
In an embodiment of the invention, the composition of the common daily ration of described brood time and the common daily ration of finishing period is as table 1.
Experimental results show that, with the feed broiler chicken of 1 age in days of the daily ration of broiler that contains probiotics of the present invention, the chicken body weight that the daily ration of broiler that contains the daily ration of broiler of 0.015% probiotics of the present invention, the daily ration of broiler that contains 0.025% probiotics of the present invention during 14 age in days and contain 0.05% probiotics of the present invention is fed is significantly higher than control group (P<0.05); During 21 age in days, the chicken body weight that the daily ration of broiler that contains 0.025% probiotics of the present invention and the daily ration of broiler that contains 0.05% probiotics of the present invention are fed is significantly higher than control group (P<0.05); The chicken body weight that the daily ration of broiler that contains the daily ration of broiler of 0.015% probiotics of the present invention, the daily ration of broiler that contains 0.025% probiotics of the present invention during 42 age in days and contain 0.05% probiotics of the present invention is fed is significantly higher than control group (P<0.05).The apparent digestibility of the rate of animals delivered to the slaughter-house of the chicken that the daily ration of broiler that contains 0.02% probiotics of the present invention is fed, crude protein, calcium and phosphorus and meat feed ratio all are significantly higher than control group.The broiler chicken of feeding with the daily ration of broiler that contains probiotics of the present invention, its total amino acid content is higher than control group (with the common Diet of broiler chicken), the total amount of essential amino acid is significantly higher than control group, and wherein valine, methionine, phenylalanine, cystine, tyrosine, proline content are significantly higher than control group; The broiler chicken of feeding with the daily ration of broiler that contains probiotics of the present invention, the chewiness of the crude protein content of chicken, the tender degree of chest muscle, chest muscle elasticity, chicken leg muscle and chest muscle meat all is significantly higher than control group; The broiler chicken of feeding with the daily ration of broiler that contains probiotics of the present invention, the cholesterol level of chicken, fat content and drip loss are all significantly lower than control group.
The specific embodiment
Below in conjunction with the specific embodiment, the present invention is further described in detail, the embodiment provided is only in order to illustrate the present invention, rather than in order to limit the scope of the invention.Experimental technique in following embodiment, if no special instructions, be conventional method.In following embodiment, material used, reagent etc., if no special instructions, be conventional material or reagent, all can obtain from commercial channels.
Bacillus subtilis RJGP16 in following embodiment (Deng Jun, Li Yunfeng, Yang Qian. the impact that bacillus subtilis and pig source Bacillus acidi lactici mixed feeding are grown the piglet intestinal villus. animal and veterinary medical science newspaper " 02 phase of .2013; Li Yunfeng, Deng Jun, Zhang Jin China, Deng. the impact [J] of bacillus subtilis on the local innate immunity of Small Intestine of Piglets and TLR expression. journal of animal science and veterinary medicine, 2011, (04): 562-566.), the public can obtain from the application's applicant this bacterial classification, to repeat the application's experiment.
Saccharomyces cerevisiae CICC1562 in following embodiment is purchased from Chinese industrial microorganism fungus kind preservation administrative center (China Center of Industrial Culture Collection, CICC).
Lactobacillus plantarum CICC22696 in following embodiment is purchased from Chinese industrial microorganism fungus kind preservation administrative center (China Center of Industrial Culture Collection, CICC).
The formula of the common daily ration of broiler chicken in following embodiment is as shown in table 1.
Table 1, the common daily ration of broiler chicken form and proportioning
In table 1, every kilogram of premix contains: CuSO
45H
2O 0.64g, FeSO
4H
2O 516g, ZnSO
4H
2O 4g, MnSO
4H
2O 318g, Na
2SeO
30.016g, V
A16IU, V
D3415IU, V
E400IU, V
K90mg, V
B160mg, V
B2200mg, nicotinic acid 550mg, calcium pantothenate 220mg, folic acid 12mg, all the other are stone flour.
" % " in table 1 is the quality percentage composition.
The preparation of composite bacteria agent capable of embodiment 1, broiler chicken
The present embodiment relates to two kinds of broiler chicken composite bacteria agent capables, is respectively composite bacteria agent capable A and broiler chicken composite bacteria agent capable B for broiler chicken.
Broiler chicken is that the liquid spawn of the liquid spawn of the liquid spawn of bacillus subtilis RJGP16, saccharomyces cerevisiae CICC1562 and Lactobacillus plantarum CICC22696 all is diluted to respectively to 10 with sterilized water with composite bacteria agent capable A
8After cfu/ml according to the colony forming unit number of 1:3:1 than the mixture be mixed to get.
Broiler chicken is that the liquid spawn of the liquid spawn of the liquid spawn of bacillus subtilis RJGP16, saccharomyces cerevisiae CICC1562 and Lactobacillus plantarum CICC22696 all is diluted to respectively to 10 with sterilized water with composite bacteria agent capable B
8After cfu/ml according to the colony forming unit number of 1:1:1 than the mixture be mixed to get.
Wherein, the liquid spawn of bacillus subtilis RJGP16 is to cultivate at 30-32 ℃ the culture (all substances in culture vessel) that 18-24h obtains with fermentation medium A.Concrete preparation method is as follows: the bacillus subtilis RJGP16 activated is inoculated in the 500ml triangular flask of the fermentation medium A that 100ml is housed, 30-32 ℃, 180rpm (radius of turn 20mm) shaken cultivation 20 hours, all substances (culture) in triangular flask are the liquid spawn of bacillus subtilis RJGP16.The bacillus subtilis RJGP16 content of the liquid spawn of this bacillus subtilis RJGP16 is 2 * 10
8Cfu/ml.
The liquid spawn of saccharomyces cerevisiae CICC1562 is to cultivate at 28-30 ℃ the culture (all substances in culture vessel) that 32-40h obtains with fermentation medium A.Concrete preparation method is as follows: the saccharomyces cerevisiae CICC1562 activated is inoculated in the 500ml triangular flask of the fermentation medium A that 100ml is housed, 28-30 ℃, 180rpm (radius of turn 20mm) shaken cultivation 36 hours, all substances (culture) in triangular flask are the liquid spawn of saccharomyces cerevisiae CICC1562.The saccharomyces cerevisiae CICC1562 content of the liquid spawn of this saccharomyces cerevisiae CICC1562 is 1.5 * 10
8Cfu/ml.
The liquid spawn of Lactobacillus plantarum CICC22696 is to cultivate at 35-37 ℃ the culture (all substances in culture vessel) that 46-54h obtains with fermentation medium A.Concrete preparation method is as follows: the Lactobacillus plantarum CICC22696 activated is inoculated in the 500ml triangular flask of the fermentation medium A that 100ml is housed, 35-37 ℃, standing cultivation 48 hours, all substances (culture) in triangular flask are the liquid spawn of Lactobacillus plantarum CICC22696.The Lactobacillus plantarum CICC22696 content of the liquid spawn of this Lactobacillus plantarum CICC22696 is 4.5 * 10
8Cfu/ml.
Every liter of fermentation medium A prepares as follows: 10g glucose, 10g peptone, 1.5g KH
2PO
4, 0.80g MnSO
4, 1.00g MgSO
4, 1.0g NaCl, be settled to 1000mL with distilled water, pH value 6.5; Under 121 ℃ of conditions, sterilizing is 20 minutes.
The preparation of probiotics of embodiment 2, broiler chicken
The present embodiment provides two kinds of broiler chicken probioticses, is called probiotics A and broiler chicken probiotics B for broiler chicken.
The culture that the broiler chicken that broiler chicken is the fermentation medium A cultivation embodiment 1 of employing embodiment 1 with probiotics A obtains with composite bacteria agent capable A.Concrete preparation method is as follows: the broiler chicken of EXAMPLE l is accessed in the 500L fermentation tank of the fermentation medium A that 200L embodiment 1 is housed according to the inoculum concentration (volume of the composite bacteria agent capable of access and the volume ratio of fermentation medium) of 6% (volume ratio) with composite bacteria agent capable A, at 34 ℃, throughput is 600L/h, under the condition that speed of agitator is 150rpm, cultivate 42 hours, collect the material (zymotic fluid) that in fermentation tank, all cultivations obtain, add powdered rice hulls to carry out drying at 50 ℃, obtain broiler chicken probiotics A.This broiler chicken is 6 * 10 with the total thalline content in probiotics A
9Cfu/g.Wherein, powdered rice hulls passes through micronization processes (90% powder granularity is less than 100 μ m, and average grain diameter is less than 50 μ m, and described average grain diameter is 50% diameter of particle) in advance.
The culture that the broiler chicken that broiler chicken is the fermentation medium A cultivation embodiment 1 of employing embodiment 1 with probiotics B obtains with composite bacteria agent capable B.Concrete preparation method is as follows: the broiler chicken of embodiment 1 is accessed in the 500L fermentation tank of the fermentation medium A that 200L embodiment 1 is housed according to the inoculum concentration (volume of the composite bacteria agent capable of access and the volume ratio of fermentation medium) of 6% (volume ratio) with composite bacteria agent capable B, at 34 ℃, throughput is 600L/h, under the condition that speed of agitator is 150rpm, cultivate 42 hours, collect the material (zymotic fluid) that in fermentation tank, all cultivations obtain, add powdered rice hulls to carry out drying at 50 ℃, obtain broiler chicken probiotics B.This broiler chicken is 4 * 10 with the total thalline content in probiotics B
9Cfu/g.Wherein, powdered rice hulls passes through micronization processes (90% powder granularity is less than 100 μ m, and average grain diameter is less than 50 μ m, and described average grain diameter is 50% diameter of particle) in advance.
The broiler chicken of embodiment 3, use embodiment 2 improves broiler weight with probiotics A
1.1 experimental animal
Select the love that body weight is 50 ± 5 grams to pull out increasingly--strong young 12000 of 1 age in days of fast large-scale white plumage broiler chicken is divided into 4 processed group, i.e. control group, 0.015% probiotics group, 0.025% probiotics group and 0.05% probiotics group at random.3 repetitions of each processed group, each repeats 1000 chickens.
1.2 daily ration
Broiler chicken brood time daily ration is divided into the common daily ration of broiler chicken brood time, probiotics A brood time daily ration, 0.025% broiler chicken probiotics A brood time daily ration, 0.05% broiler chicken probiotics A brood time daily ration for 0.015% broiler chicken.Wherein, the common daily ration of broiler chicken brood time is as shown in table 1; 0.015% broiler chicken is that in the common daily ration of broiler chicken brood time of table 1, to add be the daily ration that the broiler chicken of 0.015% embodiment 2 of the common daily ration quality of broiler chicken brood time of table 1 obtains with probiotics A with probiotics A brood time daily ration; 0.025% broiler chicken is that in the common daily ration of broiler chicken brood time of table 1, to add be the daily ration that the broiler chicken of 0.025% embodiment 2 of the common daily ration quality of broiler chicken brood time of table 1 obtains with probiotics A with probiotics A brood time daily ration; 0.05% broiler chicken is that in the common daily ration of broiler chicken brood time of table 1, to add be the daily ration that the broiler chicken of 0.05% embodiment 2 of grain quality of common dawn of broiler chicken brood time of table 1 obtains with probiotics A with probiotics A brood time daily ration.
Broiler chicken finishing period daily ration is divided into four kinds, the common daily ration of broiler chicken finishing period, probiotics A finishing period daily ration, 0.025% broiler chicken probiotics A finishing period daily ration, 0.05% broiler chicken probiotics A finishing period daily ration for 0.015% broiler chicken.Wherein, the common daily ration of broiler chicken finishing period is as shown in table 1; 0.015% broiler chicken is that in the common daily ration of broiler chicken finishing period of table 1, to add be the daily ration that the broiler chicken of 0.015% embodiment 2 of the common daily ration quality of broiler chicken finishing period of table 1 obtains with probiotics A with probiotics A finishing period daily ration; 0.025% broiler chicken is that in the common daily ration of broiler chicken finishing period of table 1, to add be the daily ration that the broiler chicken of 0.025% embodiment 2 of the common daily ration quality of broiler chicken finishing period of table 1 obtains with probiotics A with probiotics A finishing period daily ration; 0.05% broiler chicken is that in the common daily ration of broiler chicken finishing period of table 1, to add be the daily ration that the broiler chicken of 0.05% embodiment 2 of the common daily ration quality of broiler chicken finishing period of table 1 obtains with probiotics A with probiotics A finishing period daily ration.
1.3 test method
42 days experimental periods,, all broiler chicken free choice feedings of duration of test and drinking-water, carry out immunity according to the routine immunization program, and except the daily ration difference of feeding, other raising conditionally complete is identical.Wherein, control group is fed with the common daily ration of broiler chicken brood time of table 1 at the 1-28 age in days, at the 29-42 age in days, with the common daily ration of broiler chicken finishing period of table 1, is fed; 0.015% probiotics group is fed with probiotics A brood time daily ration with 0.015% broiler chicken at the 1-28 age in days, at the 29-42 age in days, with 0.015% broiler chicken, with probiotics A finishing period daily ration, is fed; 0.025% probiotics group is fed with probiotics A brood time daily ration with 0.025% broiler chicken at the 1-28 age in days, at the 29-42 age in days, with 0.025% broiler chicken, with probiotics A finishing period daily ration, is fed; 0.05% probiotics group is fed with probiotics A brood time daily ration with 0.05% broiler chicken at the 1-28 age in days, at the 29-42 age in days, with 0.05% broiler chicken, with probiotics A finishing period daily ration, is fed.
The body weight of broiler chicken during determination test.Assay method is: respectively at 7 ages in days, 14 ages in days, 21 ages in days, 28 ages in days, 42 ages in days, weigh respectively once, the fasting in early morning is prohibited water and is weighed.While weighing, each repeats to randomly draw 100 chickens, records respectively.
2 data statistics
Test the independent sample t check of all the data SPSS12.0 (SPSS Inc., USA) statistical software and process statistics.
3 result of the tests
The Different adding amount broiler chicken with probiotics on the impact of broiler growth in Table 2.In feeding process, the Broiler Chicken weight average of 0.025% probiotics processed group is kept above the level of other processing.During 7 age in days, broiler weight is with 0.025% probiotics processed group maximum, higher than control group, but difference not significantly (P>0.05); During 14 age in days, 0.015% probiotics group, 0.025% probiotics group, 0.05% probiotics group, the broiler chicken live-weight is significantly higher than control group (P<0.05); During 21 age in days, 0.025% probiotics group, 0.05% use probiotics group are compared with control group, and the broiler chicken live-weight is significantly higher than control group (P<0.05); During 28 age in days, the broiler chicken live-weight of 0.025% probiotics group is higher than control group, but difference not significantly (P>0.05); 35 ages in days, 0.025% probiotics group and 0.05% probiotics group show the trend that the broiler chicken live-weight is higher, but with contrast group difference not significantly (P>0.05); During 42 age in days, all interpolation broiler chicken all are significantly higher than control group (P<0.05) by the broiler chicken live-weight of probiotics group, wherein obvious with 0.025% probiotics group additive effect.Analysis-by-synthesis, broiler chicken of the present invention has good facilitation with probiotics to improving the broiler chicken live-weight, wherein best with the action effect of 0.025% addition.
Table 2 broiler chicken is the impact (g) on the broiler chicken live-weight with probiotics
Annotate: the different significance degree of The English alphabet differential of going together in table, between the processing of same letter in 0.05 level without significant difference, between the different processing of letter, in 0.05 level, there were significant differences.
The broiler chicken of embodiment 4, use embodiment 2 improves meat chicken production performance with probiotics A and broiler chicken with probiotics B
1.1 experimental animal
Select 1 age in days that body weight is 50 ± 5 Ke Aiwei mattress one white plumage broiler chicken to be good for young 9000, be divided at random three groups: control group, broiler chicken probiotics A group, broiler chicken probiotics B group, 3 repetitions of each processed group, each repeats 1000 chickens.
1.2 daily ration
Broiler chicken brood time daily ration is divided into the common daily ration of broiler chicken brood time, probiotics A brood time daily ration, broiler chicken probiotics B brood time daily ration for broiler chicken.Wherein, the common daily ration of broiler chicken brood time is as shown in table 1; Broiler chicken is that in the common daily ration of broiler chicken brood time of table 1, to add be the daily ration that the broiler chicken of 0.02% embodiment 2 of the common daily ration quality of broiler chicken brood time of table 1 obtains with probiotics A with probiotics A brood time daily ration; Broiler chicken is that in the common daily ration of broiler chicken brood time of table 1, to add be the daily ration that the broiler chicken of 0.02% embodiment 2 of the common daily ration quality of broiler chicken brood time of table 1 obtains with probiotics B with probiotics B brood time daily ration.
Broiler chicken finishing period daily ration is divided into the common daily ration of broiler chicken finishing period, probiotics A finishing period daily ration, broiler chicken probiotics B finishing period daily ration for broiler chicken.Wherein, the common daily ration of broiler chicken finishing period is as shown in table 1; Broiler chicken is that in the common daily ration of broiler chicken finishing period of table 1, to add be the daily ration that the broiler chicken of 0.02% embodiment 2 of the common daily ration quality of broiler chicken finishing period of table 1 obtains with probiotics A with probiotics A finishing period daily ration; Broiler chicken is that in the common daily ration of broiler chicken finishing period of table 1, to add be the daily ration that the broiler chicken of 0.02% embodiment 2 of the common daily ration quality of broiler chicken finishing period of table 1 obtains with probiotics B with probiotics B finishing period daily ration.
1.3 test method
46 days experimental periods,, all broiler chicken free choice feedings of duration of test and drinking-water, carry out immunity according to the routine immunization program, and except the daily ration difference of feeding, other raising conditionally complete is identical.Wherein, control group is fed with the common daily ration of broiler chicken brood time of table 1 at the 1-28 age in days, at the 29-46 age in days, with the common daily ration of broiler chicken finishing period of table 1, is fed; Broiler chicken is fed with probiotics A brood time daily ration with broiler chicken at the 1-28 age in days by probiotics A group, at the 29-46 age in days, with broiler chicken, with probiotics A finishing period daily ration, is fed; Broiler chicken is fed with probiotics B brood time daily ration with broiler chicken at the 1-28 age in days by probiotics B group, at the 29-46 age in days, with broiler chicken, with probiotics B finishing period daily ration, is fed.
The death rate of broiler chicken, the rate of animals delivered to the slaughter-house, feed intake and feedstuff-meat ratio during determination test.Gather broiler chicken excrement sample and daily ration sample in the 36th age in days to 42 age in days, measure the nutriment apparent digestibility of broiler chicken with endogenous indicator method (4mol/L ash insoluble in hydrochloric acid).Crude protein (CP) content in daily ration and excrement sample adopts crude protein determining method in the GB/T6432-1994 feed, calcium content adopts the mensuration atom absorption spectrophotometry method of NY/T1944-2010 Calcium in Feed, and phosphorus content adopts the mensuration AAS of total phosphorus in the GB/T6437-2002 feed.
Computing formula is as follows:
The death rate (%)=dead chicken number (only)/enter to give up chicken number (only) * 100;
The rate of animals delivered to the slaughter-house (%)=deliver for sale chicken number (only)/enter to give up chicken number (only) * 100;
Feed intake (kg/ only)=feed consumption (kg)/total amount of livestock for sale (only);
Feedstuff-meat ratio=feed intake (kg)/deliver for sale heavily (kg);
Diet nutrient material apparent digestibility (%)=[1-(b/a) * (c/d)] * 100.Wherein, the content (%) that a is certain nutritional labeling in daily ration; The content that b is certain nutritional labeling in ight soil (%); The content that c is ash insoluble in hydrochloric acid in daily ration (%); The content that d is ash insoluble in hydrochloric acid in ight soil (%).
2 data statistics
Test the independent sample t check of all the data SPSS12.0 (SPSS Inc., USA) statistical software and process statistics.
3 result of the tests
Table 3 is delivered the impact of counterpoise and feedstuff-meat ratio for sale for broiler chicken on broiler chicken with probiotics.When delivering for sale, control group broiler chicken counterpoise is 2.55kg, and broiler chicken to deliver counterpoise for sale by probiotics A group be 2.61kg, broiler chicken uses probiotics A group than the high 0.06kg of control group, be significantly higher than control group (P<0.05), the broiler chicken that shows embodiment 2 is conducive to improve delivering for sale heavily of broiler chicken with probiotics A, and broiler chicken is delivered for sale middle slightly high than control group with probiotics B group, but difference not remarkable (P>0.05).Known by data in table 3, in process of the test, the feedstuff-meat ratio of control group broiler chicken is the highest, is 1.98; And broiler chicken is only 1.88 by the meat feed ratio of probiotics A group broiler chicken, significantly lower than control group (P<0.05); Broiler chicken between broiler chicken is with probiotics A group and control group, has the trend that reduces feedstuff-meat ratio with the feedstuff-meat ratio of probiotics B group, but with contrast group difference not remarkable (P>0.05).Illustrate and use broiler chicken can reduce the broiler chicken feedstuff-meat ratio with probiotics A, use broiler chicken to there is the trend that reduces feedstuff-meat ratio by probiotics B group, but effect is not remarkable.
Table 3 broiler chicken is the impact on the broiler chicken feedstuff-meat ratio with probiotics
| Process | Deliver counterpoise (kg) for sale | Feedstuff-meat ratio |
| Control group | 2.55 b | 1.98 a |
| Probiotics A group for broiler chicken | 2.61 a | 1.88 b |
| Probiotics B group for broiler chicken | 2.58 ab | 1.94 ab |
Annotate: the different letters of same column mark are significant difference (P<0.05).
Broiler chicken with probiotics on the impact of the broiler chicken death rate and the rate of animals delivered to the slaughter-house in Table 4.In table, data are known, the control group death rate is 14.18%, and broiler chicken is organized the death rate significantly lower than control group (P<0.05) with probiotics A, is only 9.50%, broiler chicken reaches more than 90% by the rate of animals delivered to the slaughter-house of probiotics A group, is significantly higher than control group (P<0.05); Broiler chicken also significantly is better than control group (P<0.05) by the death rate and the rate of animals delivered to the slaughter-house of probiotics B group, but improves effect not as good as probiotics A group.Illustrate and use broiler chicken can reduce the broiler chicken death rate with probiotics A and B, increase the number of heads of livestock for sale, wherein broiler chicken is better than broiler chicken probiotics B with probiotics A.
Table 4 broiler chicken is the impact on the broiler chicken death rate with probiotics
| Process | The death rate (%) | The rate of animals delivered to the slaughter-house (%) |
| Control group | 14.18 a | 85.82 b |
| Probiotics A group for broiler chicken | 9.50 b | 90.50 a |
| Probiotics B group for broiler chicken | 10.19 b | 89.81 a |
Annotate: the different letters of same column mark are significant difference (P<0.05).
Table 5 is depicted as broiler chicken impact on the daily ration of broiler apparent digestibility with probiotics.After adding broiler chicken probiotics A, B, the apparent digestibility of protein brings up to 64.04% and 60.77% by 51.70% respectively, be significantly higher than control group (P<0.05), broiler chicken shows by probiotics A group the trend that digestibility is higher, but between two kinds of probiotics processed group without significant difference (P > 0.05); Broiler chicken also is significantly higher than control group (P<0.05) by calcium, the phosphorus digestibility of probiotics A group; Broiler chicken is compared with control group and is all had improvement trend by calcium, the phosphorus digestibility of probiotics B group, and wherein the phosphorus digestibility is significantly higher than control group (P<0.05), and calcium digestibility and control group are without significant difference (P>0.05); Broiler chicken improves the effect of calcium phosphorus digestibility not as good as probiotics A group for broiler chicken by probiotics B group.Illustrate that broiler chicken of the present invention can significantly improve the apparent digestibility of broiler chicken crude protein, calcium, phosphorus with probiotics.
Table 5 broiler chicken is the impact on the daily ration of broiler apparent digestibility with probiotics
Annotate: the different letters of same column mark are significant difference (P<0.05).
Embodiment 5, with the broiler chicken of embodiment 2, with probiotics A and broiler chicken, with probiotics B, improve Meat Quality of Broiler Chicks
1.1 experimental animal
Select 1 age in days that body weight is 50 ± 5 Ke Aiwei mattresses-Bai plumage broiler chicken to be good for young 9000, be divided at random three groups: control group, broiler chicken probiotics A group, broiler chicken probiotics B group, 3 repetitions of each processed group, each repeats 1000 chickens.
1.2 daily ration
Broiler chicken brood time daily ration is divided into the common daily ration of broiler chicken brood time, probiotics A brood time daily ration, broiler chicken probiotics B brood time daily ration for broiler chicken.Wherein, the common daily ration of broiler chicken brood time is as shown in table 1; Broiler chicken is that in the common daily ration of broiler chicken brood time of table 1, to add be the daily ration that the broiler chicken of 0.02% embodiment 2 of the common daily ration quality of broiler chicken brood time of table 1 obtains with probiotics A with probiotics A brood time daily ration; Broiler chicken is that in the common daily ration of broiler chicken brood time of table 1, to add be the daily ration that the broiler chicken of 0.02% embodiment 2 of the common daily ration quality of broiler chicken brood time of table 1 obtains with probiotics B with probiotics B brood time daily ration.
Broiler chicken finishing period daily ration is divided into the common daily ration of broiler chicken finishing period, probiotics A finishing period daily ration, broiler chicken probiotics B finishing period daily ration for broiler chicken.Wherein, the common daily ration of broiler chicken finishing period is as shown in table 1; Broiler chicken is that in the common daily ration of broiler chicken finishing period of table 1, to add be the daily ration that the broiler chicken of 0.02% embodiment 2 of the common daily ration quality of broiler chicken finishing period of table 1 obtains with probiotics A with probiotics A finishing period daily ration; Broiler chicken is that in the common daily ration of broiler chicken finishing period of table 1, to add be the daily ration that the broiler chicken of 0.02% embodiment 2 of the common daily ration quality of broiler chicken finishing period of table 1 obtains with probiotics B with probiotics B finishing period daily ration.
1.3 test method
47 days experimental periods,, all broiler chicken free choice feedings of duration of test and drinking-water, carry out immunity according to the routine immunization program, and except the daily ration difference of feeding, other raising conditionally complete is identical.Wherein, control group is fed with the common daily ration of broiler chicken brood time of table 1 at the 1-28 age in days, at the 29-47 age in days, with the common daily ration of broiler chicken finishing period of table l, is fed; Broiler chicken is fed with probiotics A brood time daily ration with broiler chicken at the 1-28 age in days by probiotics A group, at the 29-47 age in days, with broiler chicken, with probiotics A finishing period daily ration, is fed; Broiler chicken is fed with probiotics B brood time daily ration with broiler chicken at the 1-28 age in days by probiotics B group, at the 29-47 age in days, with broiler chicken, with probiotics B finishing period daily ration, is fed.
Experimental period, finish the previous day, often repeats to choose 3 of the chickens that body weight is close, and the neck bloodletting is butchered, fasting 12h before killing.Measure cholesterol level, crude protein content, amino acid content, fat content, moisture, muscle drip loss, pH in the chicken whole-body muscle; Measure yellowish pink, the tender degree of chest muscle; Utilize elasticity, viscosity, cohesion, the chewiness of instrumental test chicken wings, chicken leg and pigeon breast position muscle.
1.3.1 cholesterol level is measured according to GB/T5009.128-2003 " mensuration of Food Cholesterol " standard.
1.3.2 gross protein value is measured
Take the meat sample, then smash to pieces with tissue mashing machine, put into the baking oven constant temperature drying.Add 2g meat sample, 2.5g antioxidant and the 10mL concentrated sulfuric acid in digestion tube, then on the High-temperature Digestion stove, carry out treatments of the sample.The application kjeldahl apparatus is measured, and computing formula is:
Crude protein %=(A-A
0) * 0.014 * 6.25 * 0.1612/W * 100%
A
0For blank, W is meat sample heavy (g), and 0.1612 is sulfuric acid concentration, and A is machine-readable number.
1.3.3 crude fat content is measured
Get the meat sample, then with tissue mashing machine, smash to pieces and mix, constant temperature drying in baking oven is placed the 1g sample in filtration paper cylinder; Dry filtration paper cylinder, until weighing is constant weight, carry out the mensuration of crude fat on Milko-Tester.
1.3.4 determination of moisture
Get the meat sample, the G that weighs after smashing to pieces, put into the moisture ware and be weighed as W, then puts into baking oven, dry 4h under 250 ℃, put into drying basin, dry cooling 30min, and then weigh, be recorded as A, calculate moisture according to following formula: moisture=(W-A/G) * 100%.
1.3.5 yellowish pink, measure
Get without muscle and tread on and fatty green meat sample 25g and water 50mL, smash immediately making beating to pieces in tissue mashing machine, get it and all put into the 50mL centrifuge tube, then carry out centrifugal for the first time, getting supernatant is respectively charged in 2-3 little centrifuge tube and carries out centrifugation for the second time, centrifugal condition is centrifugal 10min under 3000r/min, after taking out centrifuge tube, remove liquid level fat with filter paper, then supernatant is placed in to cuvette (optical path 1cm), under 540nm wavelength condition, with ultraviolet specrophotometer, measures OD value.
1.3.6 the mensuration of tender degree
Put into 10g meat sample in 100mL stainless steel homogenate cup, add cold sucrose (0.24mol/L) and potassium chloride (0.2mol/L) the mixed liquor 50ml of equivalent simultaneously, standing 5min, then stir sample at full speed and cut 40s with refiner.With aperture, be 250 μ m and the strainer filtering of knowing weight, after filtration, filter screen and residue blot with blotting paper, then weigh.Then calculating can not be converted into contained residue weight in every 100g meat sample by broken section weight of meat sample (being greater than 250 μ m) of filter screen, and this residue weight is called to muscle shatter index (FI).FI can reflect Meat Tenderness, and the FI value is less, shows that muscle is more delicate.
1.3.7 the mensuration of muscle drip loss
Sample thief is weighed, and weight is W
1, then sample is placed in to the sealing polybag, place 24h in 4 ℃ of refrigerators, wipe meat surface moisture with filter paper after taking-up, then to weigh for the second time, weight is W
2.With following formula, calculated: muscle drip loss (%)=(W
1-W
2)/W
1* 100%.
1.3.8 Contents of Amino Acids
According to GB/T5009.124-2003 " amino acid whose mensuration in food " standard, measured
2 data statistics
Test the independent sample t check of all the data SPSS12.0 (SPSS Inc., USA) statistical software and process statistics.
3 result of the tests
3.1 improve the nutritional labeling of chicken muscle
Result is as shown in table 6, and the cholesterol level of broiler chicken use probiotics A group muscle and crude fat content are significantly lower than control group, and crude protein content is significantly higher than control group (P<0.05).Broiler chicken by the cholesterol level of probiotics B group and crude fat content lower than control group, but with control group without significant difference (P>0.05); Crude protein content is significantly higher than control group (P<0.05), with broiler chicken, uses probiotics A group without significant difference (P>0.05).Illustrate that the probiotics of the present invention of feeding can effectively improve the nutritional labeling of meat quality of table poultry.
Table 6 broiler chicken is the impact on the muscle nutrition composition with probiotics
| Detect index | Control group | Probiotics A group for broiler chicken | Probiotics B group for broiler chicken |
| Cholesterol (mg/100g) | 79.10±2.89 a | 76.70±2.71 b | 77.81±2.54 ab |
| Crude protein (%) | 24.71±0.21 b | 26.72±0.54 a | 26.39±0.40 a |
| Crude fat (%) | 4.46±0.19 a | 3.78±0.15 b | 3.99±0.21 ab |
Annotate: the different letters of colleague's mark are significant difference (P<0.05).
As shown in table 7, the total amino acid content that broiler chicken is organized with probiotics A is higher than control group, the total amount of essential amino acid is significantly higher than control group (P<0.05), and wherein valine, methionine, phenylalanine, cystine, tyrosine, proline content are significantly higher than control group (P<0.05).Broiler chicken also has with like the A category and improves effect by probiotics B group, and total amino acid content and TEAA are all higher than control group, and wherein TEAA is significantly higher than control group (P<0.05); Several amino acids content shows the trend higher than control group, and wherein the content of methionine, valine, cystine and tyrosine is significantly higher than control group (P<0.05).The increase of valine content, can make alanine in blood plasma raise, and improve the animal body immunity function; The increase of methionine content, can improve immunity of organisms, reduce Dietary Crude Protein matter level, improves production performance on certain degree simultaneously; The rising of lysine, can improve the gastric secretion effect, plays and improve a poor appetite, promote growth and the effect of growing, and improves the absorption of calcium and accumulation in vivo thereof, accelerates bone growth.
Table 7 broiler chicken is the impact on Amino Acid Contents of Muscle with probiotics
Annotate: the different letters of colleague's mark are significant difference (P<0.05).
3.2 improve the tender degree of chicken muscle and be outlet capacity
As shown in Table 8 and 9, each pH value, moisture and yellowish pink difference of organizing muscle is not remarkable for result; For the tender degree of chest muscle, broiler chicken is significantly higher than control group (P<0.05) with probiotics A group and chicken with the tender degree of chest muscle of probiotics B group; Broiler chicken uses the drip loss of probiotics A group and B group chest muscle significantly lower than control group (P<0.05),, illustrate that it is outlet capacity preferably that broiler chicken has by probiotics A group and B group.In addition, use probiotics to chicken pH value, moisture and yellowish pinkly also show certain improvement effect, but with contrast group difference remarkable (P > 0.05).In sum, the broiler chicken of the present invention of feeding can effectively improve the quality of chicken with probiotics.
Table 8 broiler chicken is the impact on pH, moisture and the drip loss of muscle with probiotics
| Process | The pH value | Moisture (%) | Drip loss (%) |
| Control group | 6.34±0.05 a | 74.02±0.88 a | 2.47±0.48 a |
| Probiotics A group for broiler chicken | 6.38±0.07 a | 74.12±0.39 a | 2.21±0.22 b |
| Probiotics B group for broiler chicken | 6.39±0.09 a | 74.05±0.67 a | 2.33±0.42 b |
Annotate: the different letters of same column mark are significant difference (P<0.05).
Table 9 broiler chicken with probiotics on the tender degree of chest muscle and yellowish pink impact
| Process | Tender degree (g/100g) | Yellowish pink |
| Control group | 17.45±0.05 b | 1.11±0.14 a |
| Probiotics A group for broiler chicken | 18.89±0.14 a | 1.19±0.16 a |
| Probiotics B group for broiler chicken | 18.80±0.21 a | 1.17±0.07 a |
Annotate: the different letters of same column mark are significant difference (P<0.05).
3.3 improve thoracic muscle elasticity
As shown in table 10, broiler chicken is with the elasticity of the chicken wings of probiotics A group and chicken leg muscle and control group difference remarkable (P > 0.05), elasticity for pigeon breast, broiler chicken is significantly higher than control group (P<0.05) by the elasticity of probiotics A group pigeon breast, illustrates that the broiler chicken of the present invention of feeding can effectively improve the elasticity of pigeon breast meat with probiotics.
Table 10 broiler chicken with probiotics on the flexible impact of chicken
| ? | Chicken wings | Chicken leg | Pigeon breast |
| Control group | 1.65±0.07 | 1.81±0.06 | 2.03±0.04 |
| Probiotics A group for broiler chicken | 1.71±0.24 | 2.43±0.24 | 2.77±0.08* |
Annotate: in table, mark " * " means significant difference (P<0.05).
3.4 the impact on chicken viscosity
As shown in table 11, broiler chicken is with the viscosity of chicken wings, chicken leg and the pigeon breast muscle of probiotics A group and control group difference remarkable (P > 0.05).
Table 11 broiler chicken is the impact on chicken viscosity with probiotics
| ? | Chicken wings | Chicken leg | Pigeon breast |
| Control group | 0.27±0.02 | 0.24±0.49 | 0.23±0.49 |
| Probiotics A group for broiler chicken | 0.38±0.03 | 0.18±0.01 | 0.28±0.06 |
3.5 the impact on the chicken cohesion
As shown in table 12, broiler chicken is with the cohesion of chicken wings, chicken leg and the pigeon breast muscle of probiotics A group and control group difference remarkable (P > 0.05).
Table 12 broiler chicken is the impact on the chicken cohesion with probiotics
| ? | Chicken wings | Chicken leg | Pigeon breast |
| Control group | 0.45±0.03 | 0.58±0.02 | 0.57±0.04 |
| Probiotics A group for broiler chicken | 0.45±0.03 | 0.58±0.05 | 0.59±0.02 |
3.6 improve the chewiness of chicken leg and pigeon breast muscle
As shown in table 13, broiler chicken is with the chewiness of the chicken wings muscle of probiotics A group and control group difference not significantly (P > 0.05), and the chewiness of the chicken leg that broiler chicken is organized with probiotics A and the chewiness of pigeon breast muscle is significantly higher than control group (P<0.05).Illustrate that the broiler chicken of the present invention of feeding can effectively improve the chewiness of chicken leg and pigeon breast meat with probiotics.
Table 13 broiler chicken is the impact on the chicken chewiness with probiotics
| ? | Chicken wings | Chicken leg | Pigeon breast |
| Control group | 2.45±0.32 | 1.76±0.47 | 2.61±0.36 |
| Probiotics A group for broiler chicken | 2.68±0.31 | 4.02±0.97* | 5.58±0.39* |
Annotate: mark " * " significant difference (P<0.05) in table.
Claims (10)
1. a method that improves chicken meat and production performance, comprise with the daily ration of broiler feeding dorking that contains probiotics and obtain production performance and chicken meat quality all higher than the step of the broiler chicken with the common Diet of broiler chicken, the active component of described probiotics is to cultivate with fermentation medium the culture that composite bacteria agent capable obtains; The active component of described composite bacteria agent capable is comprised of bacillus subtilis, saccharomyces cerevisiae and Lactobacillus plantarum; The described daily ration of broiler that contains probiotics is to be the feed that the described probiotics of 0.015%-0.05% of the common daily ration quality of described broiler chicken obtains to adding in the common daily ration of described broiler chicken, in the common daily ration of described broiler chicken, does not contain described probiotics.
2. method according to claim 1, it is characterized in that: described bacillus subtilis is that bacillus subtilis RJGP16, described saccharomyces cerevisiae are that saccharomyces cerevisiae CICC1562 and described Lactobacillus plantarum are Lactobacillus plantarum CICC22696.
3. method according to claim 1 and 2 is characterized in that: in the active component of described composite bacteria agent capable, the colony forming unit number of described bacillus subtilis, described saccharomyces cerevisiae and described Lactobacillus plantarum is than being 1:1-3:1.
4. according to claim 1 or 2 or 3 described methods, it is characterized in that: the thalline content of described probiotics is 5 * 10
8-8 * 10
9Cfu/g.
5. according to arbitrary described method in claim 1-3, it is characterized in that: described raising chicken meat is following A 1)-A15) in all or part of:
A1) improve the valine content of broiler chicken chicken;
A2) improve the methionine content of broiler chicken chicken;
A3) improve the lysine content of broiler chicken chicken;
A4) improve the phenylalanine content of broiler chicken chicken;
A5) improve the cystine of broiler chicken chicken;
A6) improve the tyrosine content of broiler chicken chicken;
A7) improve the proline content of broiler chicken chicken;
A8) improve the crude protein content of broiler chicken chicken;
A9) reduce the cholesterol level of broiler chicken chicken;
A10) reduce the fat content of broiler chicken chicken;
A11) reduce the drip loss of broiler chicken chicken;
A12) improve the tender degree of chest muscle of broiler chicken;
A13) improve the chest muscle elasticity of broiler chicken;
A14) improve the chewiness of chicken leg muscle;
A15) improve the chewiness of chicken chest muscle meat;
Described production performance is following B1)-B6) in whole or five kinds or four kinds or three kinds or two kinds or a kind of:
B1) rate of animals delivered to the slaughter-house;
B2) meat feed ratio;
B3) apparent digestibility of protein;
B4) apparent digestibility of calcium;
B5) apparent digestibility of phosphorus;
B6) body weight.
6. according to arbitrary described method in claim 1-5, it is characterized in that: every liter of described fermentation medium is prepared as follows: 10g carbon source, 10g nitrogenous source, 1.5g KH
2PO
4, 0.80g MnSO
4, 1.00g MgSO
4, 1.0gNaCl, water is settled to 1000mL, pH value 6.0-7.0; Described carbon source is glucose or sucrose; Described nitrogenous source is peptone or dusty yeast.
7. according to arbitrary described method in claim 1-6, it is characterized in that: described composite bacteria agent capable for by the liquid spawn of the liquid spawn of the liquid spawn of described bacillus subtilis, described saccharomyces cerevisiae and described Lactobacillus plantarum according to the colony forming unit number of 1:1-3:1 than the mixture be mixed to get.
8. according to arbitrary described method in claim 1-7, it is characterized in that: the liquid spawn of described bacillus subtilis is to cultivate at 30-32 ℃ the culture that 18-24h obtains with described fermentation medium;
The liquid spawn of described saccharomyces cerevisiae is to cultivate at 28-30 ℃ the culture that 32-40h obtains with described fermentation medium;
The liquid spawn of described Lactobacillus plantarum is to cultivate at 35-37 ℃ the culture that 46-54h obtains with described fermentation medium.
9. according to arbitrary described method in claim 1-8, it is characterized in that: the described condition of culture adopted with fermentation medium cultivation composite bacteria agent capable is: the described condition of culture adopted with fermentation medium cultivation composite bacteria agent capable is: cultivation temperature 34-36 ℃, incubation time 42-48 hour, the inoculum concentration of described composite bacteria agent capable is 6% (volume ratio).
10. according to arbitrary described method in claim 1-9, it is characterized in that: the described daily ration of broiler that contains probiotics be a) or b) or c):
A) to adding in the common daily ration of described broiler chicken, be the feed that 0.02% described probiotics of the common daily ration quality of described broiler chicken obtains;
B) to adding in the common daily ration of described broiler chicken, be the feed that 0.025% described probiotics of the common daily ration quality of described broiler chicken obtains;
C) to adding in the common daily ration of described broiler chicken, be the feed that the described probiotics of 0.02%-0.025% of the common daily ration quality of described broiler chicken obtains.
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| WO2018216818A1 (en) * | 2017-05-26 | 2018-11-29 | 日環科学株式会社 | Muscle quality improvement agent |
| CN111513207A (en) * | 2020-05-28 | 2020-08-11 | 衡阳师范学院 | Production method and use effect of biological feed capable of improving meat quality |
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| CN103416585B (en) * | 2013-08-20 | 2015-09-30 | 沈阳科丰牧业科技有限公司 | Broiler chicken probiotics is utilized to improve the method for meat chicken production performance and chicken meat quality |
| WO2018049657A1 (en) * | 2016-09-19 | 2018-03-22 | 陈云 | Healthy nutritious feed for animals |
| CN114381409B (en) * | 2022-02-24 | 2023-08-18 | 齐齐哈尔大学 | Fermented feed for improving animal productivity and/or improving feed utilization rate and application thereof |
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| CN103416585B (en) * | 2013-08-20 | 2015-09-30 | 沈阳科丰牧业科技有限公司 | Broiler chicken probiotics is utilized to improve the method for meat chicken production performance and chicken meat quality |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105614025A (en) * | 2016-01-28 | 2016-06-01 | 长春海恩得生物科技有限公司 | Micro-ecological preparation for improving feed intake of livestock and poultry and preparation method thereof |
| WO2018216818A1 (en) * | 2017-05-26 | 2018-11-29 | 日環科学株式会社 | Muscle quality improvement agent |
| JP2018199642A (en) * | 2017-05-26 | 2018-12-20 | 日環科学株式会社 | Muscle-modifying agent |
| JP7118344B2 (en) | 2017-05-26 | 2022-08-16 | 日環科学株式会社 | muscle modifier |
| CN111513207A (en) * | 2020-05-28 | 2020-08-11 | 衡阳师范学院 | Production method and use effect of biological feed capable of improving meat quality |
Also Published As
| Publication number | Publication date |
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| CN103416585B (en) | 2015-09-30 |
| CN105029080A (en) | 2015-11-11 |
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