CN103404594A - Functional milk with effect of prompting lead discharging and preparation method thereof - Google Patents
Functional milk with effect of prompting lead discharging and preparation method thereof Download PDFInfo
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Abstract
The invention belongs to the field of health products, and particularly relates to functional milk with an effect of prompting lead discharging. The functional milk comprises the following components by weight percentage: 0.0001-0.0025 percent of plant polyphenols, 0.002-0.024 percent of vitamin C, 0.2-2.4 percent of calcium lactate, 0.003-0.025 percent of zinc sulfate, 0.002-0.024 percent of copperas, and 97.5245-99.7929 percent of liquid milk. The functional milk has the benefits that the effective lead discharging component comprises the plant polyphenols, the vitamin and the necessary metal elements for a human body, does not contain substances hurting the human body with side effects on a human body, and facilitates health, and the added nutrient supplements and food additives do not affect absorption of an organism for other necessary elements including calcium, iron and zinc, and do not affect the appearance of the milk.
Description
Technical field
The invention belongs to field of health care products, particularly a kind of functional dairy products with promotion lead-eliminating effect.
Background technology
Along with the develop rapidly of the modernization of industry and transportation, plumbous more and more serious to the pollution problem of environment.Lead is a kind of to the toxic mineral element of human body without any physiological function, can cause acute toxic encephalopathy and orientation and behavior disorder to central nervous system; To digestive system, can cause apocleisis, constipation, stomachache and vomiting etc.; To blood circulation system, can cause the microcell hypochrosis microcytic anemia; To urinary system, can cause interstitial nephritis, renal tubule pathology etc.Children are due to the restriction that is subjected to self resistance and the packed food that often contacts toy, edible leaded filling food, beverage, candy, lime-preserved egg, puffed rice and greasepaint, add the impact that causes the environmental factors such as use of lead contamination in atmosphere and the leaded paint of house fitting and coating because of industrial discharge, in environment and daily life, plumbous absorption is adult's several times.Therefore children are acute sufferers of Effects of Lead Exposure, have a strong impact on it and grow.
Prevent at present and treat saturnine method to comprise dietotherapy, take the approach such as lead discharging class health food and medicine.Wherein dietotherapy aspect, take in the abundant fruit and vegetable of some all kinds of nutrient contents more and can prevent lead poisoning and improve Pb-B.Lead-removing health care food aspect, Chinese invention patent 97109256.7 disclose a kind of take seaweed extract, edible compound amino acid, be rich in ascorbic Chinese gooseberry extract and contain the oral liquid of zinc sunflower seeds extract as primary raw material; 98118072.8 disclose the oral nutrient lead-expelling powdered granules by vitamin C, Cobastab, organic zinc, compound amino acid, methionine, half fat propylhomoserin, pearl powder composition; 03118470.7 disclose a kind of half fat propylhomoserin, vitamin C and B, thiosulfonic acid, zinc gluconate, chrysanthemum medlar water lead discharging medicine or functional food as active ingredient that adopt; 200510020093.5 disclosing a kind of is the nutritive lead discharging oral liquid of active ingredient by low-ester pectin, sodium alginate, taurine, vitamin C, zinc gluconate; 200510035019.0 disclose a kind of chewing tablet for discharging lead that contains glutathione, vitamin C, taurine, immunoglobulin (Ig), zinc, CPP and pearl powder; 02132494.8 disclose, a kind ofly take shitosan, chitosan oligosaccharide, Radix Glycyrrhizae, tuckahoe extracts and be main active ingredient, sodium alginate, pectin, vitamin C, zinc gluconate, calcium gluconae are the lead-removing health care food of auxiliary material; 200610014294.9 disclosing a kind of is the health-care food matrix of raw material by milk powder, sugar, vegetable fat powder, pvp1, sodium carboxymethyl starch, Ethylenediaminetetraacetic Acid Calcium Salt, NaFeEDTA sodium, honey element, milk sheet essence, ultra-fine SiO2, natrium citricum and citric acid; 200810025609.9 disclose a kind of health food that promotes lead-expelling function and auxiliary improving memory function that has, raw material is spina date seed, Poria cocos, intelligence development, konjaku flour, vitamin C 4 ~ 44, zinc citrate.In existing lead discharging drug invention patent, be substantially all to adopt various traditional Chinese medicinal components with lead-expelling function, as the fruit of Rangoon creeper (200610035739.1), bracken flavone (200710019471.7), Lygodium japonicum (200910011410.5), sour Punica granatum L. extract (201010100276.9) etc., or adopt the Chinese medicine composition (201110202025.6,201110265420.9,201110303673.0,201010107031.9) of many complexity.
As can be known from the patent of invention of above-mentioned existing lead-removing health care food, be mainly liquid oral or the oral product of solid lead discharging, as preventative lead-removing health care food, children's acceptance is lower, and medicine is generally also only for the severe Lead Poisoning Patients.Therefore, from the angle of child health care, develop the food with lead-expelling function that is applicable to children's taste and hobby, have wide market development potential and commercial value.Dairy products are also the food that liked by children, develop the lead-expelling function dairy products that a kind of children of being suitable for and teenager drink, and make it to have concurrently comparatively significant lead discharging effect and desirable flavor taste, significant.
Summary of the invention
Purpose of the present invention is to develop the lead-expelling function dairy products that a kind of children of being suitable for and teenager drink, and has comparatively significant lead discharging effect and desirable flavor taste.
Technical scheme for achieving the above object is: a kind of functional dairy products with promotion lead-eliminating effect, comprise that plant polyphenol, vitamin C, calcium lactate, zinc sulfate, ferrous sulfate, liquid milk are formulated, each composition proportioning by weight is as follows: plant polyphenol 0.0001% ~ 0.0025%, vitamin C 0.002% ~ 0.024%, calcium lactate 0.2% ~ 2.4%, zinc sulfate 0.003% ~ 0.025%, ferrous sulfate 0.002 ~ 0.024% and liquid milk 97.5245% ~ 99.7929%.
As preferably, described plant polyphenol is one or more mixtures in Tea Polyphenols, apple polyphenol and vine polyphenol.
As preferably, the optimum weight proportioning of active ingredient is: plant polyphenol 0.0015%, vitamin C 0.018%, calcium lactate 1.8%, zinc sulfate 0.019%, ferrous sulfate 0.018% and liquid milk 98.1435%.
As preferably, described plant polyphenol is that Tea Polyphenols, apple polyphenol and vine polyphenol mix according to weight ratio 2:1:2.
A kind of method with the functional dairy products that promote lead-eliminating effect for preparing, plant polyphenol, vitamin C, calcium lactate, zinc sulfate, ferrous sulfate are added in liquid milk by formula, after fully mixing, make functional liquid dairy product, be dried into milk powder or be pressed into the milk sheet according to common process.
Beneficial effect of the present invention is:
1, in lead discharging active ingredient of the present invention, comprise plant polyphenol, vitamin and Essential metal elements of human, do not contain the material that human body is existed to side effect, body health benefits;
2,, when reaching the lead discharging purpose, do not affect the absorption of body for other indispensable element calcium, iron, zinc;
3, when reaching the lead discharging purpose, the nutrition fortifier added and food additives do not affect the sense organ of dairy products, are easy to be accepted by the consumer.
The accompanying drawing explanation
Fig. 1 is that the dynamic metabolism of respectively organizing Pb-B after modeling changes schematic diagram.
The specific embodiment
Below in conjunction with zoopery, body test-meal experiment and specific embodiment, the effect of lead discharging health care dairy products of the present invention to lead discharging is described further, but protection scope of the present invention is not limited in this:
Zoopery:
1, the preparation of lead discharging dairy products and the grouping of metabolism animal:
Animal used as test type selecting and raising: animal is selected clean level ICR mouse, and male, body weight is 28 ~ 32 g, purchased from Medical College of Zhejiang Univ.'s animal center.Mouse is at temperature (20 ± 1) ° C, and relative humidity is to raise in 40% ~ 60% Animal House.Mouse feeds and raises basal feed, and basal feed is prepared according to standard GB/T/T 20195-2006 " preparation of animal feed, sample ".Before experiment, fasting is 12 hours, can freely drink deionized water.Consider that this experiment relates to the detection of metal ion, for avoiding unnecessary interference, the water that during whole zoopery, mouse is drunk is deionized water.
Animal used as test grouping: adopt clean level ICR mouse, be divided into 7 groups, every group 12, be respectively: blank group, high blood lead model control group, positive controls, common dairy products group (not adding any food additives and nutrition fortifier), low dosage lead discharging dairy products group, middle dosage lead discharging dairy products group and high dose lead discharging dairy products group.Each food additives and the nutrition fortifier additive capacity of 4th ~ 7 groups are as shown in table 1, and wherein the plant polyphenol mixture mixes according to Tea Polyphenols, apple polyphenol and vine polyphenol weight ratio 2:1:2.
Table 1
2, mouse metabolism experiment:
1) the mouse Animal House adapts to a week.
2) make high blood lead model:
The preparation of plumbous contamination agent: adopt lead acetate solution, according to documents and materials, to be 12.8mg/kg(be 7mg/kg if take lead to the contamination reference dose), so compound concentration is the lead acetate water solution of 1.28 mg/mL, lumbar injection dosage is 0.1 mL/10g body weight.
Contamination animal group: 6 groups except the blank group, totally 72,8:30 every morning carries out lumbar injection in left and right in experimental period, and mouse can be drunk deionized water.
3) blood sampling mensuration and animal heavily divide into groups
After finishing, modeling carries out the blood sampling of live body eye socket, the second day of arrangement of time after modeling finishes;
Adopt graphite oven atomic absorption to measure lead, iron, zinc, calcium level in blood sample by national standard;
According to plumbous measurement result, take > lead concentration of 0.5mg/L is the standard of modeling success, reject lead concentration<0.4mg/L and the mouse of 1mg/L, and consider respectively to organize the balance of lead concentration, again 6 groups are carried out to the mouse arrangement.
4) lead discharging metabolism
Again after mark was carried out in grouping, 8:30 every morning carried out the gavage experiment in left and right, and experimental subjects is for removing blank group and model control group, and wherein positive controls is filled with and raised EDTACaNa
2, common, basic, normal, high test group is filled with respectively and is raised corresponding milk, and blank group and model control group are filled with deionized water, and gavage dosage is the 0.1mL/10g body weight.
Wherein, the blood sampling of live body eye socket is carried out at the second weekend in six weeks and 4th week end, and blood sample is detected, and is mainly the research of dynamic metabolism aspect.
Extract eyeball after the gavage end and get blood and put to death mouse the 6th weekend, standard according to metabolism, consider the position of possibility enriched lead in body, blood sample, brain, liver, kidney, the femur of getting every mouse detect, and detecting index is still lead, iron, zinc, calcium level.
3, experimental result:
1) blood lead dynamic metabolism:
Fig. 1 has shown after the modeling success dynamic metabolism curve of respectively organizing Pb-B.As can be seen from the figure, except the Pb-B of blank group normally, other each group makes its Pb-B all more than 2 mg/L because of high blood lead model; From on trend, As time goes on each Pb-B of organizing mouse all reduces.Although As time goes on the Pb-B of model control group also reduces, during by the 63rd day, its Pb-B is still more than 1.5 mg/L.The Pb-B of positive controls, common dairy products group and three experimental group compares with the model control group of same period the decline all had in various degree, during by the 63rd day, all these Pb-Bs of five groups all almost fall back to original level, with the blank group without significant difference (p > 0.05).In addition, the lead discharging rate of basic, normal, high dosage group, in all time periods, it is the highest that plumbous rate falls in the mouse of drinking the dairy products of middle dosage interpolation level, yet it is not the highest that plumbous rate falls in the mouse of drinking the dairy products of high dose interpolation level, even plumbous rate falls in the mouse lower than the dairy products of drinking low dosage interpolation level.
2) Pb-B of Main Tissues organ:
After gavage finished, we detected the lead level in the mouse Main Tissues organ that easily causes plumbous enrichment, and result is as table 2(n=6) as shown in.
Table 2
As can be seen from Table 2, each dosage group has been compared significant difference (p<0.05) at liver with the lead level in kidney with model control group, be that lead is absorbed in Mice Body and walks the blood urine approach, and drink the lead level of liver and kidney in the mouse of lead discharging dairy products and obviously reduce.But each dosage group is compared no significant difference (p > 0.05) in the lead level at the position of these two easy enriched leads of femur and brain with model group.Because this experimental period is long, reach two months, lead ion has time enough to accumulate to some " profound level " histoorgans such as brain and femur at the Mice Body intracellular metabolite, and the experimental result explanation is in case lead is accumulated at these two positions, and these lead discharging dairy products are just without obvious effect.
3) on the impact of calcium, iron, zinc and body weight:
Except the Pb-B of test Main Tissues organ, we measure calcium, iron, zinc level in blood and above-mentioned each histoorgan simultaneously.All results show, the equal no significant difference (p > 0.05) between each experimental group and control group of three kinds of microelement levels in blood and each histoorgan, this illustrates that each dosage group lead discharging dairy products do not affect absorption and the metabolism of mouse to other three kinds of trace elements in its lead discharging effect of performance.
In addition, whole experimentation small mouse growing state is normal, is not subject to the impact of high blood lead modeling and active material gavage, and model control group and each experimental mice body weight are compared there was no significant difference (p > 0.05) with the blank group.
4, conclusion:
From the result of metabolism, can draw to draw a conclusion:
1, As time goes on each dosage group Pb-B descends, common dairy products, low middle high dose lead discharging dairy products all have the lead discharging effect of obstructed degree, the lead discharging effect of low, middle dosage lead discharging dairy products is better than common dairy products, and the lead discharging effect of middle dosage lead discharging dairy products is the most outstanding.
2, each dosage group all can reduce level plumbous in mouse liver and kidney effectively, but can not reduce the lead content of accumulating in femur and brain.
3, each dosage group lead discharging dairy products do not affect absorption and the metabolism of mouse to calcium, iron, three kinds of trace elements of zinc in its lead discharging effect of performance.
The human experiment experiment:
1, explanation:
The first, the edible safety of each composition of the functional dairy products of promotion lead discharging proposed by the invention has all obtained demonstration.Wherein, Tea Polyphenols is in standard GB/T 2760-2011, to allow to use, and apple polyphenol and vine polyphenol also all have pertinent literature to studies have shown that its biological safety (1. T Shoji etc., The toxicology and safety of apple polyphenol extract, Food and Chemical Toxicology; 2. grandson waits quietly, the safety experiment research of apple polyphenol, People's Armed Police's medical science; 3. Wren, AF etc., 90-Day Oral Toxicity Study of a Grape Seed Extract (IH636) in Rats, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY; 4. Shen Ji is red etc., the toxicity test of vine polyphenol, toxicology magazine).In addition, vitamin C, calcium lactate, zinc sulfate and ferrous sulfate are all that national standard allows the nutrition fortifier used.Therefore, from each composition, raw material of the present invention is safe to human body.
The second, the legitimacy of human experiment experiment obtains assessment and the demonstration of relevant human experimentation Ethics Committee;
The 3rd, the object of all participation human experimentations is introduced to this experiment, the participation of all experimental subjects is all the principle of voluntariness, before participating in the experiment, signs Informed Consent Form.
2. test-meal object:
Preschool and the school-ager's (3-12 year) in human experimentation Object Selection industrial park, be divided at random 13 groups, every group of 20 people, be respectively: blank group, common dairy products group, positive controls, low dose group, middle dosage group, high dose group, 1 group of embodiment, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment, 5 groups of embodiment, 6 groups of embodiment, 7 groups of embodiment, wherein positive controls is commercially available " the precious lead discharging oral liquid of virgin health ".3 months experimental periods of test-meal, gather blood and hair sample after 3 months finish, and measures lead, calcium, iron, zinc content, and result is as shown in table 3.
Table 3
From data table 3, can find out, the lead discharging effect of middle dosage group is best, with respect to the blank group, and dropping blood lead and send out rate plumbous all more than 60%.Wherein, common dairy products are to lead discharging is not acted on, and other groups have certain lead-eliminating effect.From the level of blood calcium, blood iron blood lead, common dairy products, positive controls and lead discharging dairy products and the equal no significant difference of blank group (p > 0.05).Therefore, lead discharging dairy products proposed by the invention, in effective lead discharging, do not affect the picked-up of the important minerals such as calcium, iron, zinc.
Embodiment 1:
In every kilogram of liquid milk, add Tea Polyphenols 8 mg/kg, vitamin C 100 mg/kg, calcium lactate 11000 mg/kg, zinc sulfate 160 mg/kg, ferrous sulfate 90 mg/kg add, after fully dissolving mixing, make functional liquid dairy product, be dried into milk powder or be pressed into milk sheet etc. according to common process.
Sensory testing's result: a little sediment is arranged, and mouthfeel is general.
The rate test result: the dropping blood lead rate is 6.8%, falls that to send out rate plumbous be 7.3%, the lead discharging DeGrain.
Embodiment 2:
In every kilogram of liquid milk, add apple polyphenol 15 mg/kg, vitamin C 120 mg/kg, calcium lactate 18000 mg/kg, zinc sulfate 190 mg/kg, ferrous sulfate 180 mg/kg add, after fully dissolving mixing, make functional liquid dairy product, be dried into milk powder or be pressed into milk sheet etc. according to common process.
Sensory testing's result: a little sediment is arranged, and mouthfeel is general.
Lead discharging rate test result: the dropping blood lead rate is 21.3%, falls that to send out rate plumbous be 19.1%, and the lead discharging effect still can.
Embodiment 3:
In every kilogram of liquid milk, add vine polyphenol 15 mg/kg, vitamin C 180 mg/kg, calcium lactate 6000 mg/kg, zinc sulfate 190 mg/kg, ferrous sulfate 180 mg/kg add, after fully dissolving mixing, make functional liquid dairy product, be dried into milk powder or be pressed into milk sheet etc. according to common process.
Sensory testing's result: a little sediment is arranged, and mouthfeel is general.
Lead discharging rate test result: the dropping blood lead rate is 28.7%, falls that to send out rate plumbous be 26.4%, and the lead discharging effect still can.
Embodiment 4:
In every kilogram of liquid milk, add Tea Polyphenols 7.5 mg/kg, vine polyphenol 7.5mg/kg, vitamin C 180 mg/kg, calcium lactate 18000 mg/kg, zinc sulfate 70 mg/kg, ferrous sulfate 180 mg/kg add, and after fully dissolving mixing, make functional liquid dairy product, be dried into milk powder or be pressed into milk sheet etc. according to common process.
Sensory testing's result: deposit-free, mouthfeel is better.
Lead discharging rate test result: the dropping blood lead rate is 43.8%, falls that to send out rate plumbous be 40.4%, and the lead discharging effect is better.
Embodiment 5:
In every kilogram of liquid milk, add Tea Polyphenols 7.5 mg/kg, apple polyphenol 7.5 mg/kg, vitamin C 180 mg/kg, calcium lactate 18000 mg/kg, zinc sulfate 190 mg/kg, ferrous sulfate 130 mg/kg add, and after fully dissolving mixing, make functional liquid dairy product, be dried into milk powder or be pressed into milk sheet etc. according to common process.
Sensory testing's result: deposit-free, mouthfeel is better.
Lead discharging rate test result: the dropping blood lead rate is 46.1%, falls that to send out rate plumbous be 42.5%, and the lead discharging effect is better.
Embodiment 6:
In every kilogram of liquid milk, add Tea Polyphenols 6 mg/kg, apple polyphenol 3 mg/kg, vine polyphenol 6mg/kg, vitamin C 180 mg/kg, calcium lactate 18000 mg/kg, zinc sulfate 190 mg/kg, ferrous sulfate 180 mg/kg add, after fully dissolving mixing, make functional liquid dairy product, be dried into milk powder or be pressed into milk sheet etc. according to common process.
Sensory testing's result: deposit-free, mouthfeel is mellow.
Lead discharging rate test result: the dropping blood lead rate is 68.3%, falls that to send out rate plumbous be 65.7%, the lead discharging best results.
Embodiment 7:
In every kilogram of liquid milk, add Tea Polyphenols 5 mg/kg, apple polyphenol 10 mg/kg, vine polyphenol 5mg/kg, vitamin C 180 mg/kg, calcium lactate 18000 mg/kg, zinc sulfate 190 mg/kg, ferrous sulfate 180 mg/kg add, after fully dissolving mixing, make functional liquid dairy product, be dried into milk powder or be pressed into milk sheet etc. according to common process.
Sensory testing's result: deposit-free, mouthfeel is mellow.
Lead discharging rate test result: the dropping blood lead rate is 62.7%, falls that to send out rate plumbous be 61.3%, and the lead discharging effect is fine.
Claims (5)
1. one kind has the functional dairy products that promote lead-eliminating effect, it is characterized in that comprising: plant polyphenol, vitamin C, calcium lactate, zinc sulfate, ferrous sulfate, liquid milk are formulated, and each composition proportioning by weight is as follows: plant polyphenol 0.0001% ~ 0.0025%, vitamin C 0.002% ~ 0.024%, calcium lactate 0.2% ~ 2.4%, zinc sulfate 0.003% ~ 0.025%, ferrous sulfate 0.002 ~ 0.024% and liquid milk 97.5245% ~ 99.7929%.
2. a kind of functional dairy products that promote lead-eliminating effect that have according to claim 1, is characterized in that, described plant polyphenol is one or more mixtures in Tea Polyphenols, apple polyphenol and vine polyphenol.
3. a kind of functional dairy products that promote lead-eliminating effect that have according to claim 1 and 2, it is characterized in that, the optimum weight proportioning of active ingredient is: plant polyphenol 0.0015%, vitamin C 0.018%, calcium lactate 1.8%, zinc sulfate 0.019%, ferrous sulfate 0.018% and liquid milk 98.1435%.
4. a kind of functional dairy products that promote lead-eliminating effect that have according to claim 3, is characterized in that, described plant polyphenol is that Tea Polyphenols, apple polyphenol and vine polyphenol mix according to weight ratio 2:1:2.
5. one kind prepares the method with the functional dairy products that promote lead-eliminating effect, it is characterized in that, plant polyphenol, vitamin C, calcium lactate, zinc sulfate, ferrous sulfate are added in liquid milk by formula, after fully mixing, make functional liquid dairy product, be dried into milk powder or be pressed into the milk sheet according to common process.
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| CN103976432A (en) * | 2014-04-17 | 2014-08-13 | 五河县鲲鹏食品饮料有限公司 | Lygodium japonicum (thunb.) sw lead-removing health beverage and preparation method thereof |
| CN104322668A (en) * | 2014-11-15 | 2015-02-04 | 柳州市康小乐牛奶有限公司 | Preparation method of yogurt containing grape-seed polyphenols |
| CN104322669A (en) * | 2014-11-15 | 2015-02-04 | 柳州市康小乐牛奶有限公司 | Preparation method of yogurt containing apple polyphenols |
| CN105166047A (en) * | 2015-08-14 | 2015-12-23 | 江西人之初乳品营养有限公司 | Children nutrition formula milk product capable of promoting lead excretion |
| CN108477303A (en) * | 2018-06-28 | 2018-09-04 | 陕西秦王乳业有限公司 | A kind of full-cream high-calcium milk powder and preparation method thereof |
| CN111358003A (en) * | 2020-04-28 | 2020-07-03 | 西北大学 | Compound preparation for removing lead |
| CN113439784A (en) * | 2021-06-08 | 2021-09-28 | 华南理工大学 | Polyphenol-containing dairy products and production method thereof |
| CN115813882A (en) * | 2022-12-06 | 2023-03-21 | 成都市丸天科技有限责任公司 | A method for enhancing absorption of vitamins and minerals in intestinal tract |
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| CN103918795A (en) * | 2014-04-10 | 2014-07-16 | 陕西科技大学 | Tea polyphenols probiotics goat milk tablet and preparation method thereof |
| CN103976432A (en) * | 2014-04-17 | 2014-08-13 | 五河县鲲鹏食品饮料有限公司 | Lygodium japonicum (thunb.) sw lead-removing health beverage and preparation method thereof |
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| CN108477303A (en) * | 2018-06-28 | 2018-09-04 | 陕西秦王乳业有限公司 | A kind of full-cream high-calcium milk powder and preparation method thereof |
| CN111358003A (en) * | 2020-04-28 | 2020-07-03 | 西北大学 | Compound preparation for removing lead |
| CN113439784A (en) * | 2021-06-08 | 2021-09-28 | 华南理工大学 | Polyphenol-containing dairy products and production method thereof |
| CN115813882A (en) * | 2022-12-06 | 2023-03-21 | 成都市丸天科技有限责任公司 | A method for enhancing absorption of vitamins and minerals in intestinal tract |
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Application publication date: 20131127 |