CN103399015A - Pathological section scanner and method and device for measuring locating precision of slide glass platform - Google Patents
Pathological section scanner and method and device for measuring locating precision of slide glass platform Download PDFInfo
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Abstract
The invention relates to a pathological section scanner and a method and device for measuring locating precision of a slide glass platform. The method comprises the steps of loading a characteristic section on the slide glass platform; controlling the slide glass platform to move towards a first appointed position, and acquiring a first image of the characteristic section; after the slide glass platform leaves from the first appointed position, controlling the slide glass platform to move towards the first appointed position again; after the slide glass platform is repeatedly located for m times, acquiring a second image of the characteristic section; comparing the first image with the second image; and calculating the repeated locating precision of the slide glass platform according to the resolution ratio of platform locating equipment and offset of n characteristic points. According to the method, the images of the characteristic section on the slide glass platform are obtained through a camera device provided by the pathological section scanner, the change of the position of laser equipment or infrared equipment is prevented from influencing a measuring result, and therefore the measuring accuracy is improved.
Description
Technical field
The present invention relates to the Medical Instruments technical field, particularly relate to a kind of pathological section scanner and slide glass platform positioning precision measuring method and device.
Background technology
Optical microscope, as a kind of strong instrument of exploring microworld, has been applied in every medical diagnosis and scientific experiment research very early.In recent years, along with the fast development of computing machine treatment technology and image processing techniques, the technology such as automatic acquisition of microscopical online control, micro-image had been realized.Numeral pathological section scanner also is born thereupon.
(be called for short: the slide glass platform) precision of location has very high requirement to numeral pathological section scanner, and deviation is slightly arranged, and just likely causes pathological section scanning unclear for high precision slide glass platform.And digital pathological section scanner needs the constantly position of conversion slide glass platform when scanning.Therefore, the accurate location of slide glass platform is particularly important, and precision how to measure the resetting of digital pathological section scanner slide glass platform is a problem of needing solution badly.
Whether prior art is usually utilized laser or infrared ray to detect the slide glass platform and whether has been moved to assigned address, accurate with this resetting that further judges the slide glass platform.
The shortcoming that such scheme exists is: in measuring process, the position that needs to guarantee laser equipment or infrored equipment does not change.Due to the placement error value of slide glass platform normally at micron level or even Nano grade, therefore, the micro-displacement of laser equipment or infrored equipment all will bring huge error to measurement, this has brought inconvenience to surveying work, careless slightlyly during operation just likely causes measurement result inaccurate.Simultaneously, owing to additionally having set up laser equipment or infrored equipment, therefore also caused the cost of measuring equipment higher.
Summary of the invention
What the present invention solved is digital pathological section scanner slide glass platform repetitive positioning accuracy measuring method in prior art, in measuring process, easily because of the change of laser equipment or infrored equipment position, causes the technical matters of measuring error.
In order to address the above problem, the embodiment of the present invention provides a kind of pathological section scanner slide glass platform positioning precision measuring method, comprising:
On described slide glass platform, load characteristic slice, described characteristic slice comprises n unique point, and n is natural number;
Controlling described slide glass platform moves to the first assigned address;
By being arranged on picture pick-up device on described pathological section scanner, obtain the first image of described characteristic slice, the relative position of described picture pick-up device and described pathological section scanner is constant, the positional information of described unique point before the resetting of the described slide glass platform of described the first recording image;
Control after described slide glass platform leaves described the first assigned address, then control described slide glass platform and move to described the first assigned address, this step is carried out m time, and m is natural number;
After m time is controlled the resetting of described slide glass platform, by described picture pick-up device, obtain the second image of described characteristic slice, the positional information of described unique point after the resetting of the described slide glass platform of described the second recording image;
Described the first image and described the second image are compared, to draw the side-play amount that corresponds respectively to a described n unique point;
According to the resolution of platform positioning equipment and the side-play amount of a described n unique point, calculate the precision of described slide glass platform resetting, described platform positioning equipment is used to indicate the movement of described slide glass platform.
Optionally, described characteristic slice is on white background, to be printed on the section of black cross, and described characteristic slice comprises 5 unique points, usings the center of described cross and 4 end points as described 5 unique points.
Optionally, described pathological section scanner is digital pathological section scanner, and described picture pick-up device is digital photographing apparatus, and described the first image and described the second image are digital picture.
Optionally, according to following formula, calculate the precision of described slide glass platform resetting:
Wherein, A
xPrecision, A on the expression horizontal ordinate
yPrecision on the expression ordinate, n represents the quantity of described unique point, R
xPlatform positioning equipment resolution on the expression horizontal ordinate, R
yPlatform positioning equipment resolution on the expression ordinate,
The horizontal ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The horizontal ordinate sum that represents the front described n unique point of described slide glass platform resetting,
The ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The ordinate sum that represents the front described n unique point of described slide glass platform resetting.
In order to address the above problem, the embodiment of the present invention also provides a kind of pathological section scanner slide glass platform positioning precision measurement mechanism, comprising:
Loading unit, on described slide glass platform, loading characteristic slice, described characteristic slice comprises n unique point, n is natural number;
The first control module, for after described loading unit executable operations, control described slide glass platform and move to the first assigned address;
The first image acquisition unit, for after described the first control module executable operations, by being arranged on picture pick-up device on described pathological section scanner, obtain the first image of described characteristic slice, the relative position of described picture pick-up device and described pathological section scanner is constant, the positional information of described unique point before the resetting of the described slide glass platform of described the first recording image;
The second control module, for after described the first image acquisition unit executable operations, after described slide glass platform leaves described the first assigned address, then control described slide glass platform and move to described the first assigned address, and this step is carried out m time, and m is natural number;
The second image acquisition unit, for after described the second control module is controlled the resetting of described slide glass platform m time, by described picture pick-up device, obtain the second image of described characteristic slice, the positional information of described unique point after the resetting of the described slide glass platform of described the second recording image;
Comparing unit, after described the second image acquisition unit executable operations, compare described the first image and described the second image, to draw the side-play amount that corresponds respectively to a described n unique point;
Computing unit, for after described comparing unit executable operations, according to the resolution of platform positioning equipment and the side-play amount of a described n unique point, calculate the precision of described slide glass platform resetting, described platform positioning equipment is used to indicate the movement of described slide glass platform.
Optionally, described characteristic slice is on white background, to be printed on the section of black cross, and described characteristic slice comprises 5 unique points, usings the center of described cross and 4 end points as described 5 unique points.
Optionally, described pathological section scanner is digital pathological section scanner, and described picture pick-up device is digital photographing apparatus, and described the first image and described the second image are digital picture.
Optionally, according to following formula, calculate the precision of described slide glass platform resetting:
Wherein, A
xPrecision, A on the expression horizontal ordinate
yPrecision on the expression ordinate, n represents the quantity of described unique point, R
xPlatform positioning equipment resolution on the expression horizontal ordinate, R
yPlatform positioning equipment resolution on the expression ordinate,
The horizontal ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The horizontal ordinate sum that represents the front described n unique point of described slide glass platform resetting,
The ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The ordinate sum that represents the front described n unique point of described slide glass platform resetting.
In order to address the above problem, the embodiment of the present invention also provides a kind of pathological section scanner, comprise the picture pick-up device that is arranged on described pathological section scanner, the relative position of described picture pick-up device and described pathological section scanner is constant, described picture pick-up device, for obtaining the image of the characteristic slice on the slide glass platform that is carried in described pathological section scanner, also comprises above-mentioned pathological section scanner slide glass platform positioning precision measurement mechanism.
Compared with prior art, technical scheme of the present invention has following beneficial effect:
The picture pick-up device that carries by the pathological section scanner obtains the image of the characteristic slice on the slide glass platform, the impact of having avoided the change due to laser equipment or infrored equipment position to cause measurement result, thereby improved the accuracy of measuring, simultaneously, also reduced the cost of measuring equipment.
Further, described characteristic slice is on white background, to be printed on the section of black cross, usings the center of described cross and 4 end points as described 5 unique points, thus make the location of unique point and read more accurate, more convenient.
Further, described picture pick-up device is digital photographing apparatus, and described the first image and described the second image are digital picture, take pixel as unit, calculates, thereby makes the measurement of precision can reach pixel scale, has further improved the accuracy of measuring.
The accompanying drawing explanation
Fig. 1 is pathological section scanner schematic diagram in the embodiment of the present invention;
Fig. 2 is pathological section scanner slide glass platform positioning precision measuring method process flow diagram in the embodiment of the present invention one;
Fig. 3 is pathological section scanner slide glass platform positioning precision measurement mechanism structured flowchart in the embodiment of the present invention two.
Embodiment
As shown in Figure 1, in the embodiment of the present invention, described pathological section scanner comprises slide glass platform 102 and picture pick-up device 104, and described slide glass platform 102 is positioned on workbench 101, on described slide glass platform 102, loads characteristic slice 103; Described picture pick-up device 104 is positioned at described slide glass platform 102 tops, sees through object lens 105 and obtains from top to bottom the image that is carried in the characteristic slice 103 on described slide glass platform 102; Described picture pick-up device 104 is arranged on described pathological section scanner, and in measuring process, described picture pick-up device 104 does not change with the relative position of described pathological section scanner.
The scheme that provides in the embodiment of the present invention, for the slide glass platform resetting of pathological section scanner is measured in the precision of same position, that is: first control the slide glass platform and leave the first assigned address, controlling described slide glass platform moves to described the first assigned address again, more described slide glass platform is before carrying out above-mentioned resetting operation and after having carried out above-mentioned resetting operation, whether can be positioned at same position, record the error amount of resetting, and, in conjunction with the resolution of platform positioning equipment, calculate the precision of resetting.
From such scheme, can find out, in the embodiment of the present invention, the picture pick-up device that carries by the pathological section scanner obtains the image of the characteristic slice on the slide glass platform, the impact of having avoided the change due to laser equipment or infrored equipment position to cause measurement result, thereby improved the accuracy of measuring, simultaneously, also reduced the cost of measuring equipment.
For those skilled in the art being understood better and realizing the present invention, referring to accompanying drawing, by specific embodiment, be elaborated.
Embodiment mono-
With reference to pathological section scanner slide glass platform positioning precision measuring method process flow diagram shown in Figure 2, below by concrete steps, be elaborated:
S201, load characteristic slice on described slide glass platform, described characteristic slice comprises n unique point, and n is natural number.
In order to make follow-up data read work more accurate, more convenient, therefore can adopt characteristic slice to measure.The image that foreground and background colour contrast are larger can be set on described characteristic slice, and on described image, the corner of pattern can be sharper keen.For example, can using on white background and be printed on the section of black cross as characteristic slice.On described characteristic slice, get n unique point, n is natural number, for example, can get the center of described cross and 4 end points as unique point, amounts to 5 unique points.
By such scheme, can find out, in the present embodiment, described characteristic slice is on white background, to be printed on the section of black cross, usings the center of described cross and 4 end points as described 5 unique points, thus make the location of unique point and read more accurate, more convenient.
Be understandable that, described characteristic slice is not limited to be printed on the section of specific pattern, can adopt any section, as long as can in described section, get several points as unique point.Generally, the quantity of the unique point of getting is more, and the accuracy of measurement is just higher.
S202, control described slide glass platform and move to the first assigned address.
Described the first assigned address is not limited to specific position, as long as described picture pick-up device can obtain the image of the characteristic slice on described slide glass platform.Consider the middle part that normally described slide glass platform is placed in to workbench in actual use procedure, therefore, described the first assigned address can be certain position at described workbench middle part.
In concrete enforcement, described workbench and described pathological section scanner can be integrated, can be also that described pathological section scanner removably is fixed on described workbench.In test process, the position of described workbench and described pathological section scanner does not change.
S203, by being arranged on picture pick-up device on described pathological section scanner, obtain the first image of described characteristic slice, the relative position of described picture pick-up device and described pathological section scanner is constant, the positional information of described unique point before the resetting of the described slide glass platform of described the first recording image.
Described pathological section scanner carries picture pick-up device, and in test process, the relative position of described picture pick-up device and described pathological section scanner does not change.Described picture pick-up device is positioned at described slide glass platform top, sees through object lens and obtains from top to bottom the image that is carried in the characteristic slice on described slide glass platform.
In order to simulate better the situation of described pathological section scanner in practical application, in concrete enforcement, described picture pick-up device can be arranged on by the optical microscope of described pathological section scanner, and with described optical microscope towards identical direction.
By such scheme, can find out, in the present embodiment, the picture pick-up device that carries by the pathological section scanner obtains the image of the characteristic slice on the slide glass platform, the impact of having avoided the change due to laser equipment or infrored equipment position to cause measurement result, thereby improved the accuracy of measuring, simultaneously, also reduced the cost of measuring equipment.
Described picture pick-up device obtains the action of image can carry out according to operator's instruction, and described operator's instruction can be assigned by the button that is arranged on described pathological section scanner, also can assign by the method for software.
The image that picture pick-up device obtains can be stored in the storer of external computing machine, and the work such as follow-up comparison, calculating also can be carried out by external computing machine.
Be understandable that, described pathological section scanner also can self-contained storing devices and processor, thereby no longer needs external computing machine, can realize voluntarily corresponding function.
S204, control after described slide glass platform leaves described the first assigned address, then control described slide glass platform and move to described the first assigned address, and this step is carried out m time, and m is natural number.
Control after described slide glass platform leaves described the first assigned address, then control described slide glass platform and move to described the first assigned address, this step is " resetting ".
In order to reduce the impact of front one-time positioning on follow-up location, this step can be carried out m time, and m is natural number.In concrete enforcement, it can be hundreds of times or thousands of times.
S205, after m time is controlled the resetting of described slide glass platform, obtain the second image of described characteristic slice, the positional information of described unique point after the resetting of the described slide glass platform of described the second recording image by described picture pick-up device.
Described the second image is used for comparing at subsequent step and described the first image, and then calculates the precision of described slide glass platform resetting.The obtain manner of described the second image and storage mode and described the first image are similar.
S206, compare described the first image and described the second image, to draw the side-play amount that corresponds respectively to a described n unique point.
Identify the unique point on described the first image and described the second image, obtain the side-play amount of described unique point in the position on described the first image and the position on described the second image, each unique point, corresponding to a side-play amount, therefore, can obtain n side-play amount.
Due to the two dimensional image that is of described the first image and described the second recording image, therefore, can set up two-dimensional coordinate system, obtain respectively the side-play amount of described unique point on the side-play amount on the x axle and y axle.In order to simplify calculating, can get orthogonal x axle and y axle.
In the situation that described picture pick-up device is digital photographing apparatus, can calculate described side-play amount by pixel is poor.For example, when picture pick-up device adopted 40 times of object lens to take pictures, on the image that described picture pick-up device is caught, every 100 pixels represented on subject the length (on image, every 1 millimeter is 100 pixels) of 25 microns.
By such scheme, can find out, in the present embodiment, described picture pick-up device is digital photographing apparatus, described the first image and described the second image are digital picture, take pixel as unit, calculate, thereby make the measurement of precision can reach pixel scale, further improved the accuracy of measuring.
S207, calculate the precision of described slide glass platform resetting according to the resolution of platform positioning equipment and the side-play amount of a described n unique point, and described platform positioning equipment is used to indicate the movement of described slide glass platform.
In concrete enforcement, described platform positioning equipment can be grating scale.Grating is a thin slice that is comprised of strip lens, and grating scale can be used to indicate the movement of described slide glass platform.The resolution of grating scale has determined the unit distance of slide glass platform movement, has also further determined the maximum error value that slide glass platform of every movement may cause.For example, when adopting resolution, be the grating scale of 0.05 micron while indicating described slide glass platform movement, slide glass platform of every movement, maximum possible causes the error of 0.05 micron.By calculating the ratio of described side-play amount with respect to described maximum error value, can draw the precision of described slide glass platform resetting.
In concrete enforcement, can adopt following formula to calculate the precision of described slide glass platform resetting:
Wherein, A
xPrecision, A on the expression horizontal ordinate
yPrecision on the expression ordinate, n represents the quantity of described unique point, R
xPlatform positioning equipment resolution on the expression horizontal ordinate, R
yPlatform positioning equipment resolution on the expression ordinate,
The horizontal ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The horizontal ordinate sum that represents the front described n unique point of described slide glass platform resetting,
The ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The ordinate sum that represents the front described n unique point of described slide glass platform resetting.
Embodiment bis-
With reference to pathological section scanner slide glass platform positioning precision measurement mechanism structured flowchart shown in Figure 3, described measurement mechanism comprises: loading unit, the first control module, the first image acquisition unit, the second control module, the second image acquisition unit, comparing unit and computing unit, wherein:
Loading unit, on described slide glass platform, loading characteristic slice, described characteristic slice comprises n unique point, n is natural number;
The first control module, for after described loading unit executable operations, control described slide glass platform and move to the first assigned address;
The first image acquisition unit, for after described the first control module executable operations, by being arranged on picture pick-up device on described pathological section scanner, obtain the first image of described characteristic slice, the relative position of described picture pick-up device and described pathological section scanner is constant, the positional information of described unique point before the resetting of the described slide glass platform of described the first recording image;
The second control module, for after described the first image acquisition unit executable operations, after described slide glass platform leaves described the first assigned address, then control described slide glass platform and move to described the first assigned address, and this step is carried out m time, and m is natural number;
The second image acquisition unit, for after described the second control module is controlled the resetting of described slide glass platform m time, by described picture pick-up device, obtain the second image of described characteristic slice, the positional information of described unique point after the resetting of the described slide glass platform of described the second recording image;
Comparing unit, after described the second image acquisition unit executable operations, compare described the first image and described the second image, to draw the side-play amount that corresponds respectively to a described n unique point;
Computing unit, for after described comparing unit executable operations, according to the resolution of platform positioning equipment and the side-play amount of a described n unique point, calculate the precision of described slide glass platform resetting, described platform positioning equipment is used to indicate the movement of described slide glass platform.
By such scheme, can find out, in the present embodiment, the picture pick-up device that carries by the pathological section scanner obtains the image of the characteristic slice on the slide glass platform, the impact of having avoided the change due to laser equipment or infrored equipment position to cause measurement result, thereby improved the accuracy of measuring, simultaneously, also reduced the cost of measuring equipment.
In concrete enforcement, described characteristic slice is on white background, to be printed on the section of black cross, and described characteristic slice comprises 5 unique points, usings the center of described cross and 4 end points as described 5 unique points.
By such scheme, can find out, in the present embodiment, described characteristic slice is on white background, to be printed on the section of black cross, usings the center of described cross and 4 end points as described 5 unique points, thus make the location of unique point and read more accurate, more convenient.
In concrete enforcement, described pathological section scanner is digital pathological section scanner, and described picture pick-up device is digital photographing apparatus, and described the first image and described the second image are digital picture.
By such scheme, can find out, in the present embodiment, described picture pick-up device is digital photographing apparatus, described the first image and described the second image are digital picture, take pixel as unit, calculate, thereby make the measurement of precision can reach pixel scale, further improved the accuracy of measuring.
In concrete enforcement, can adopt following formula to calculate the precision of described slide glass platform resetting:
Wherein, A
xPrecision, A on the expression horizontal ordinate
yPrecision on the expression ordinate, n represents the quantity of described unique point, R
xPlatform positioning equipment resolution on the expression horizontal ordinate, R
yPlatform positioning equipment resolution on the expression ordinate,
The horizontal ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The horizontal ordinate sum that represents the front described n unique point of described slide glass platform resetting,
The ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The ordinate sum that represents the front described n unique point of described slide glass platform resetting.
Embodiment tri-
As shown in Figure 1, in the present embodiment, described pathological section scanner comprises slide glass platform 102 and picture pick-up device 104, and described slide glass platform 102 is positioned on workbench 101, on described slide glass platform 102, loads characteristic slice 103; Described picture pick-up device 104 is positioned at described slide glass platform 102 tops, sees through object lens 105 and obtains from top to bottom the image that is carried in the characteristic slice 103 on described slide glass platform 102; Described picture pick-up device 104 is arranged on described pathological section scanner, and in measuring process, described picture pick-up device 104 does not change with the relative position of described pathological section scanner.
Described pathological section scanner also comprises, and the pathological section scanner slide glass platform positioning precision measurement mechanism that provides in two is provided, and adopts the pathological section scanner slide glass platform positioning precision measuring method that provides in embodiment mono-to measure the precision of slide glass platform resetting.
In concrete enforcement, described pathological section scanner can be digital pathological section scanner.
By such scheme, can find out, in the present embodiment, the picture pick-up device that carries by the pathological section scanner obtains the image of the characteristic slice on the slide glass platform, the impact of having avoided the change due to laser equipment or infrored equipment position to cause measurement result, thereby improved the accuracy of measuring, simultaneously, also reduced the cost of measuring equipment.
One of ordinary skill in the art will appreciate that all or part of step in the whole bag of tricks of above-described embodiment is to come the hardware that instruction is relevant to complete by program, this program can be stored in a computer-readable recording medium, and storage medium can comprise: ROM, RAM, disk or CD etc.
Although the present invention discloses as above, the present invention not is defined in this.Any those skilled in the art, without departing from the spirit and scope of the present invention, all can make various changes or modifications, so protection scope of the present invention should be as the criterion with the claim limited range.
Claims (9)
1. a pathological section scanner slide glass platform positioning precision measuring method, is characterized in that, comprising:
On described slide glass platform, load characteristic slice, described characteristic slice comprises n unique point, and n is natural number;
Controlling described slide glass platform moves to the first assigned address;
By being arranged on picture pick-up device on described pathological section scanner, obtain the first image of described characteristic slice, the relative position of described picture pick-up device and described pathological section scanner is constant, the positional information of described unique point before the resetting of the described slide glass platform of described the first recording image;
Control after described slide glass platform leaves described the first assigned address, then control described slide glass platform and move to described the first assigned address, this step is carried out m time, and m is natural number;
After m time is controlled the resetting of described slide glass platform, by described picture pick-up device, obtain the second image of described characteristic slice, the positional information of described unique point after the resetting of the described slide glass platform of described the second recording image;
Described the first image and described the second image are compared, to draw the side-play amount that corresponds respectively to a described n unique point;
According to the resolution of platform positioning equipment and the side-play amount of a described n unique point, calculate the precision of described slide glass platform resetting, described platform positioning equipment is used to indicate the movement of described slide glass platform.
2. pathological section scanner slide glass platform positioning precision measuring method as claimed in claim 1, it is characterized in that, described characteristic slice is on white background, to be printed on the section of black cross, described characteristic slice comprises 5 unique points, usings the center of described cross and 4 end points as described 5 unique points.
3. pathological section scanner slide glass platform positioning precision measuring method as claimed in claim 1, it is characterized in that, described pathological section scanner is digital pathological section scanner, and described picture pick-up device is digital photographing apparatus, and described the first image and described the second image are digital picture.
4. pathological section scanner slide glass platform positioning precision measuring method as claimed in claim 1, is characterized in that, according to following formula, calculates the precision of described slide glass platform resetting:
Wherein, A
xPrecision, A on the expression horizontal ordinate
yPrecision on the expression ordinate, n represents the quantity of described unique point, R
xPlatform positioning equipment resolution on the expression horizontal ordinate, R
yPlatform positioning equipment resolution on the expression ordinate,
The horizontal ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The horizontal ordinate sum that represents the front described n unique point of described slide glass platform resetting,
The ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The ordinate sum that represents the front described n unique point of described slide glass platform resetting.
5. a pathological section scanner slide glass platform positioning precision measurement mechanism, is characterized in that, comprising:
Loading unit, on described slide glass platform, loading characteristic slice, described characteristic slice comprises n unique point, n is natural number;
The first control module, for after described loading unit executable operations, control described slide glass platform and move to the first assigned address;
The first image acquisition unit, for after described the first control module executable operations, by being arranged on picture pick-up device on described pathological section scanner, obtain the first image of described characteristic slice, the relative position of described picture pick-up device and described pathological section scanner is constant, the positional information of described unique point before the resetting of the described slide glass platform of described the first recording image;
The second control module, for after described the first image acquisition unit executable operations, after described slide glass platform leaves described the first assigned address, then control described slide glass platform and move to described the first assigned address, and this step is carried out m time, and m is natural number;
The second image acquisition unit, for after described the second control module is controlled the resetting of described slide glass platform m time, by described picture pick-up device, obtain the second image of described characteristic slice, the positional information of described unique point after the resetting of the described slide glass platform of described the second recording image;
Comparing unit, after described the second image acquisition unit executable operations, compare described the first image and described the second image, to draw the side-play amount that corresponds respectively to a described n unique point;
Computing unit, for after described comparing unit executable operations, according to the resolution of platform positioning equipment and the side-play amount of a described n unique point, calculate the precision of described slide glass platform resetting, described platform positioning equipment is used to indicate the movement of described slide glass platform.
6. pathological section scanner slide glass platform positioning precision measurement mechanism as claimed in claim 5, it is characterized in that, described characteristic slice is on white background, to be printed on the section of black cross, described characteristic slice comprises 5 unique points, usings the center of described cross and 4 end points as described 5 unique points.
7. pathological section scanner slide glass platform positioning precision measurement mechanism as claimed in claim 5, it is characterized in that, described pathological section scanner is digital pathological section scanner, and described picture pick-up device is digital photographing apparatus, and described the first image and described the second image are digital picture.
8. pathological section scanner slide glass platform positioning precision measurement mechanism as claimed in claim 5, is characterized in that, according to following formula, calculates the precision of described slide glass platform resetting:
Wherein, A
xPrecision, A on the expression horizontal ordinate
yPrecision on the expression ordinate, n represents the quantity of described unique point, R
xPlatform positioning equipment resolution on the expression horizontal ordinate, R
yPlatform positioning equipment resolution on the expression ordinate,
The horizontal ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The horizontal ordinate sum that represents the front described n unique point of described slide glass platform resetting,
The ordinate sum that represents a described n unique point after the resetting of described slide glass platform,
The ordinate sum that represents the front described n unique point of described slide glass platform resetting.
9. pathological section scanner, it is characterized in that, comprise the picture pick-up device that is arranged on described pathological section scanner, the relative position of described picture pick-up device and described pathological section scanner is constant, described picture pick-up device, for obtaining the image of the characteristic slice on the slide glass platform that is carried in described pathological section scanner, also comprises the described pathological section scanner of any one slide glass platform positioning precision measurement mechanism in claim 5 to 8.
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| CN103731587A (en) * | 2013-12-18 | 2014-04-16 | 宁波江丰生物信息技术有限公司 | Digital section scanning and positioning system |
| CN104296656A (en) * | 2014-09-30 | 2015-01-21 | 苏州天准精密技术有限公司 | Device, apparatus and method for positioning measurement reference plane of measured object |
| CN103676976B (en) * | 2013-12-23 | 2016-01-13 | 中国地质科学院地质研究所 | The bearing calibration of three-dimensional working platform resetting error |
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| CN108320276A (en) * | 2017-01-13 | 2018-07-24 | 广州康昕瑞基因健康科技有限公司 | Image taking alignment method and system |
| CN108537830A (en) * | 2017-03-02 | 2018-09-14 | 广州康昕瑞基因健康科技有限公司 | Method for registering images and system and image taking alignment method and system |
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| CN108573126A (en) * | 2017-03-10 | 2018-09-25 | 广州康昕瑞基因健康科技有限公司 | XY platform displacement error detection methods and system |
| CN107831681A (en) * | 2017-11-14 | 2018-03-23 | 宁波江丰生物信息技术有限公司 | A kind of slice scanner section loads breakage-proof system and method |
| CN109979588A (en) * | 2019-01-22 | 2019-07-05 | 北京金智拓科技有限公司 | Image scanning pathological section system |
| CN112501271A (en) * | 2020-11-19 | 2021-03-16 | 武汉华大智造科技有限公司 | Slide platform leveling method, detection device and storage medium |
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| CN112904048B (en) * | 2021-03-06 | 2022-03-01 | 苏州青云瑞晶生物科技有限公司 | Method for adjusting center position of transmission electron microscope sample |
| CN114963982A (en) * | 2022-05-17 | 2022-08-30 | 山东志盈医学科技有限公司 | Method and device for detecting slice position in digital pathological slice scanner |
| CN114963982B (en) * | 2022-05-17 | 2024-05-17 | 山东志盈医学科技有限公司 | Method and device for detecting slice position in digital pathological slice scanner |
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Denomination of invention: Positioning accuracy measurement method and device of pathological section scanner and its film platform Effective date of registration: 20210302 Granted publication date: 20151014 Pledgee: China Co. truction Bank Corp Yuyao branch Pledgor: KONFOONG BIOTECH INTERNATIONAL Co.,Ltd. Registration number: Y2021330000164 |
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Application publication date: 20131120 Assignee: Hangzhou Zhituan Information Technology Co.,Ltd. Assignor: KONFOONG BIOTECH INTERNATIONAL Co.,Ltd. Contract record no.: X2024980014392 Denomination of invention: Pathological section scanner and its slide platform positioning accuracy measurement method and device Granted publication date: 20151014 License type: Common License Record date: 20240909 |