CN103380935A - Corn high-F-ratio-oligopeptide beverage and preparation method thereof - Google Patents
Corn high-F-ratio-oligopeptide beverage and preparation method thereof Download PDFInfo
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Abstract
The invention provides a corn high-F-ratio-oligopeptide beverage and a preparation method thereof. The method comprises the following steps: with corn protein meal as a basic material, adding drinking water and carrying out pulp refining so as to prepare corn protein slurry with solid content of 20 to 25%; heating the corn protein slurry to a temperature of 55 DEG C, adding 0.8 L of Neutrase accounting for 1 to 1.2% of the weight of solids in the corn protein slurry, carrying out hydrolysis with the temperature maintained, then heating to a temperature of 100 DEG C and maintaining the temperature for 20 min; after cooling, inoculating a secondary bacillus subtilis seed accounting for 5% of the weight of the corn protein slurry, maintaining the temperature and carrying out ventilating fermentation; heating to a temperature of 100 DEG C and maintaining the temperature for 20 min; carrying out filtering by using a plate and frame filter, taking a clear liquid and adding the substances consisting of, on the basis of the weight of the clear liquid, 1.2% of citric acid, 5% of granulated sugar, 0.01% of potassium acetyl sulphonate, 0.2% of sodium carboxymethylcellulose and 0.1% of corn essence; after complete dissolution of the above-mentioned substances, carrying out homogenization, filling, sterilization and the like so as to prepare a finished product.
Description
(1) technical field
The present invention relates to a kind of corn high F value oligopeptide beverage and preparation method thereof, be specifically related to adopt enzymolysis and biofermentation to combine and produce the method for corn high F value oligopeptide beverage, belong to the fermented food field.
(2) background technology
1.45 hundred million tons of China's corn annual productions, occupy the second in the world, be only second to the U.S., but because the comprehensive utilization degree to corn is inadequate, lack high-tech content, high added value and the huge precision corn deep processed product of market potential, up to the present, zein can only be sold at a bargain or discharging naturally as feed, not only polluted environment but also caused the waste of protein resource, China's Maize Industry advantage is not also given full play of.Contain the hydrophobic amino acids such as a high proportion of leucine, isoleucine, valine, alanine in corn protein, seldom contain the basic amino acids such as lysine, the odds ratio of branched-amino acid content and aromatic amino acid content is higher, and the amino acid of this uniqueness forms becomes zein to have the source of eliminating or alleviating the high F value oligopeptide of the multiple physiologically actives such as hepatic encephalopathy symptom, antifatigue effect, alleviation of alcohol are poisoning, inhibition cancer cell propagation.
The present invention adopts zymolysis technique to combine with biofermentation technique, prepares a kind of beverage that is rich in high F value oligopeptide, and long-term drinking is significant to safeguarding body health.
(3) summary of the invention
The object of the invention is to provide a kind of corn high F value oligopeptide beverage and preparation method thereof.
The object of the present invention is achieved like this: in the present invention, related percentage is mass ratio except separately having indicating, and product of the present invention adopts such method to prepare:
1. the selection of proteolytic enzyme
At first the present invention selects proteolytic enzyme to carry out partial hydrolysis to the zein slurry, finally selects the letter Neutrase of Novi 0.8 L neutral proteinase through test of many times, and enzyme work is: the 0.8AU/ gram.
2. the selection of fermentative microorganism
The zein slurry of the present invention after with Neutrase 0.8 L neutral proteinase hydrolysis carries out bacillus subtilis and continues fermentation, and the microorganism of selection is purchased from Chinese industrial microorganism fungus kind preservation center, strain name: bacillus subtilis
Bacillus subtilis, numbering: 10036.
3. the preparation of corn high F value oligopeptide beverage
3.1 corn protein powder pretreatment
Take corn protein powder as basic material, add appropriate drinking water, use the colloid mill defibrination, make solid content and be 20%-25% zein slurry.
3.2 protease hydrolytic
The zein slurry is warming up to 55 ℃, presses 1-1.2% of zein slurry solid content weight and add Neutrase 0.8 L neutral proteinase, 55 ℃-58 ℃ insulations are hydrolyzed 18-22 hours, per hour stir in hydrolytic process 10 minutes.
3.3 microbial fermentation
3.3.1 the preparation of microorganism seed
3.3.1.1 the preparation of microorganism seed culture medium
Wheat bran 5%, beancake powder 3%, surplus is distilled water, after mentioned component mixes, adds 0.2% sodium hydroxide solution, boiled 1 hour, the hydrochloric acid PH7 that neutralizes, after dissolving, standby.
3.3.1.2 first order seed preparation
Measure culture medium 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, in all pouring the freeze-dried vaccine powder in the bacillus subtilis ampulla into culture medium under germ-free condition, cultivated 48 hours at 30 ℃, 180 rev/mins shaking tables, as first order seed.
3.3.1.3 secondary seed preparation
Press the inoculum concentration inoculation first order seed of culture medium weight 10%, 30 ℃, 180 rev/mins shaking tables were cultivated 48 hours, as secondary seed.
3.3.2 microbial fermentation
To be warming up to 100 ℃ through the zein slurry that protease hydrolytic is processed, keep 20 minutes, then be cooled to 30 ℃, press 5% inoculation secondary seed of zein slurry weight, 28-30 ℃, ventilation 90-100m
3/ hour, fermentation time: 72-76 hour.
3.3.3 the preparation of corn high F value oligopeptide beverage
Will be through the zein slurry of 3.3.2 step process; be warming up to 100 ℃, kept 20 minutes, then filter with flame filter press; get the clear liquid after filtration; press the weight of clear liquid and add following material: citric acid 1.2%, granulated sugar 5%, acetyl potassium sulfonate 0.01%; sodium carboxymethylcellulose 0.2%; corn essence 0.1% after the dissolving, is made finished product through techniques such as homogeneous, can, sterilizations fully.
Adopt the method to prepare corn high F value oligopeptide beverage, principle is: protease hydrolytic part zein is low molecular peptide class and amino acid, for bacillus subtilis provides good nitrogenous source, bacillus subtilis produces the specificity peptase during the fermentation, act on zein, it is degraded to the solubility oligopeptides, because of the higher aromatic amino acid content of branched-amino acid content in the amino acid that forms zein lower, therefore in the solubility oligopeptides that obtains after microbial fermentation, branched-chain amino acid and fragrant family amino acid ratio are higher, i.e. high F value oligopeptide.
(4) specific embodiment
For a more detailed description to the present invention below in conjunction with specific embodiment:
Embodiment one:
1. the selection of proteolytic enzyme
At first the present invention selects proteolytic enzyme to carry out partial hydrolysis to the zein slurry, finally selects the letter Neutrase of Novi 0.8 L neutral proteinase through test of many times, and enzyme work is: the 0.8AU/ gram.
2. the selection of fermentative microorganism
The zein slurry of the present invention after with Neutrase 0.8 L neutral proteinase hydrolysis carries out bacillus subtilis and continues fermentation, and the microorganism of selection is purchased from Chinese industrial microorganism fungus kind preservation center, strain name: bacillus subtilis
Bacillus subtilis, numbering: 10036.
3. the preparation of corn high F value oligopeptide beverage
3.1 corn protein powder pretreatment
Take corn protein powder as basic material, add appropriate drinking water, use the colloid mill defibrination, make solid content and be 20%-25% zein slurry.
3.2 protease hydrolytic
The zein slurry is warming up to 55 ℃, presses 1% of zein slurry solid content weight and add Neutrase 0.8 L neutral proteinase, 55 ℃-58 ℃ insulations are hydrolyzed 18-22 hours, per hour stir in hydrolytic process 10 minutes.
3.3 microbial fermentation
3.3.1 the preparation of microorganism seed
3.3.1.1 the preparation of microorganism seed culture medium
Wheat bran 5%, beancake powder 3%, surplus is distilled water, after mentioned component mixes, adds 0.2% sodium hydroxide solution, boiled 1 hour, the hydrochloric acid PH7 that neutralizes, after dissolving, standby.
3.3.1.2 first order seed preparation
Measure culture medium 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, in all pouring the freeze-dried vaccine powder in the bacillus subtilis ampulla into culture medium under germ-free condition, cultivated 48 hours at 30 ℃, 180 rev/mins shaking tables, as first order seed.
3.3.1.3 secondary seed preparation
Press the inoculum concentration inoculation first order seed of culture medium weight 10%, 30 ℃, 180 rev/mins shaking tables were cultivated 48 hours, as secondary seed.
3.3.2 microbial fermentation
To be warming up to 100 ℃ through the zein slurry that protease hydrolytic is processed, keep 20 minutes, then be cooled to 30 ℃, press 5% inoculation secondary seed of zein slurry weight, 28-30 ℃, ventilation 90-100m
3/ hour, fermentation time: 72-76 hour.
3.3.3 the preparation of corn high F value oligopeptide beverage
Will be through the zein slurry of 3.3.2 step process; be warming up to 100 ℃, kept 20 minutes, then filter with flame filter press; get the clear liquid after filtration; press the weight of clear liquid and add following material: citric acid 1.2%, granulated sugar 5%, acetyl potassium sulfonate 0.01%; sodium carboxymethylcellulose 0.2%; corn essence 0.1% after the dissolving, is made finished product through techniques such as homogeneous, can, sterilizations fully.
Embodiment two:
1. the selection of proteolytic enzyme
At first the present invention selects proteolytic enzyme to carry out partial hydrolysis to the zein slurry, finally selects the letter Neutrase of Novi 0.8 L neutral proteinase through test of many times, and enzyme work is: the 0.8AU/ gram.
2. the selection of fermentative microorganism
The zein slurry of the present invention after with Neutrase 0.8 L neutral proteinase hydrolysis carries out bacillus subtilis and continues fermentation, and the microorganism of selection is purchased from Chinese industrial microorganism fungus kind preservation center, strain name: bacillus subtilis
Bacillus subtilis, numbering: 10036.
3. the preparation of corn high F value oligopeptide beverage
3.1 corn protein powder pretreatment
Take corn protein powder as basic material, add appropriate drinking water, use the colloid mill defibrination, make solid content and be 20%-25% zein slurry.
3.2 protease hydrolytic
The zein slurry is warming up to 55 ℃, presses 1.2% of zein slurry solid content weight and add Neutrase 0.8 L neutral proteinase, 55 ℃-58 ℃ insulations are hydrolyzed 18-22 hours, per hour stir in hydrolytic process 10 minutes.
3.3 microbial fermentation
3.3.1 the preparation of microorganism seed
3.3.1.1 the preparation of microorganism seed culture medium
Wheat bran 5%, beancake powder 3%, surplus is distilled water, after mentioned component mixes, adds 0.2% sodium hydroxide solution, boiled 1 hour, the hydrochloric acid PH7 that neutralizes, after dissolving, standby.
3.3.1.2 first order seed preparation
Measure culture medium 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, in all pouring the freeze-dried vaccine powder in the bacillus subtilis ampulla into culture medium under germ-free condition, cultivated 48 hours at 30 ℃, 180 rev/mins shaking tables, as first order seed.
3.3.1.3 secondary seed preparation
Press the inoculum concentration inoculation first order seed of culture medium weight 10%, 30 ℃, 180 rev/mins shaking tables were cultivated 48 hours, as secondary seed.
3.3.2 microbial fermentation
To be warming up to 100 ℃ through the zein slurry that protease hydrolytic is processed, keep 20 minutes, then be cooled to 30 ℃, press 5% inoculation secondary seed of zein slurry weight, 28-30 ℃, ventilation 90-100m
3/ hour, fermentation time: 72-76 hour.
3.3.3 the preparation of corn high F value oligopeptide beverage
Will be through the zein slurry of 3.3.2 step process; be warming up to 100 ℃, kept 20 minutes, then filter with flame filter press; get the clear liquid after filtration; press the weight of clear liquid and add following material: citric acid 1.2%, granulated sugar 5%, acetyl potassium sulfonate 0.01%; sodium carboxymethylcellulose 0.2%; corn essence 0.1% after the dissolving, is made finished product through techniques such as homogeneous, can, sterilizations fully.
Claims (1)
1. corn high F value oligopeptide method for preparing beverage is characterized in that it is to be prepared according to the following steps:
1) selection of proteolytic enzyme
At first the present invention selects proteolytic enzyme to carry out partial hydrolysis to the zein slurry, finally selects the letter Neutrase of Novi 0.8 L neutral proteinase through test of many times, and enzyme work is: the 0.8AU/ gram.
2) selection of fermentative microorganism
The zein slurry of the present invention after with Neutrase 0.8 L neutral proteinase hydrolysis carries out bacillus subtilis and continues fermentation, and the microorganism of selection is purchased from Chinese industrial microorganism fungus kind preservation center, strain name: bacillus subtilis
Bacillus subtilis, numbering: 10036.
3) corn protein powder pretreatment
Take corn protein powder as basic material, add appropriate drinking water, use the colloid mill defibrination, make solid content and be 20%-25% zein slurry.
4) protease hydrolytic
The zein slurry is warming up to 55 ℃, presses 1-1.2% of zein slurry solid content weight and add Neutrase 0.8 L neutral proteinase, 55 ℃-58 ℃ insulations are hydrolyzed 18-22 hours, per hour stir in hydrolytic process 10 minutes.
5) microbial fermentation
5.1) preparation of microorganism seed
5.1.1) preparation of microorganism seed culture medium
Wheat bran 5%, beancake powder 3%, surplus is distilled water, after mentioned component mixes, adds 0.2% sodium hydroxide solution, boiled 1 hour, the hydrochloric acid PH7 that neutralizes, after dissolving, standby.
5.1.2) the first order seed preparation
Measure culture medium 200ml in 1 500ml triangular flask, 121 ℃ of sterilizations were cooled to 30 ℃ in 20 minutes, in all pouring the freeze-dried vaccine powder in the bacillus subtilis ampulla into culture medium under germ-free condition, cultivated 48 hours at 30 ℃, 180 rev/mins shaking tables, as first order seed.
5.1.3) the secondary seed preparation
Press the inoculum concentration inoculation first order seed of culture medium weight 10%, 30 ℃, 180 rev/mins shaking tables were cultivated 48 hours, as secondary seed.
5.2) microbial fermentation
To be warming up to 100 ℃ through the zein slurry that protease hydrolytic is processed, keep 20 minutes, then be cooled to 30 ℃, press 5% inoculation secondary seed of zein slurry weight, 28-30 ℃, ventilation 90-100m
3/ hour, fermentation time: 72-76 hour.
5.3) preparation of corn high F value oligopeptide beverage
Will be through 5.2) the zein slurry of step process; be warming up to 100 ℃, kept 20 minutes, then filter with flame filter press; get the clear liquid after filtration; press the weight of clear liquid and add following material: citric acid 1.2%, granulated sugar 5%, acetyl potassium sulfonate 0.01%; sodium carboxymethylcellulose 0.2%; corn essence 0.1% after the dissolving, is made finished product through techniques such as homogeneous, can, sterilizations fully.
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| CN104543330A (en) * | 2014-12-09 | 2015-04-29 | 浙江海洋学院 | Method for preparing high F ratio oligopeptide by fermenting ground sleeve-fish by using bacillus natto |
| CN107136369A (en) * | 2017-05-04 | 2017-09-08 | 青岛灯塔味业有限公司 | One kind clarification beverage of fig juice and preparation method thereof |
| CN109797182A (en) * | 2019-03-29 | 2019-05-24 | 江苏俊启生物科技股份有限公司 | A kind of preparation method of corn peptide |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103549020A (en) * | 2013-11-18 | 2014-02-05 | 哈尔滨艾克尔食品科技有限公司 | Method for processing peanut protein milk |
| CN104543330A (en) * | 2014-12-09 | 2015-04-29 | 浙江海洋学院 | Method for preparing high F ratio oligopeptide by fermenting ground sleeve-fish by using bacillus natto |
| CN107136369A (en) * | 2017-05-04 | 2017-09-08 | 青岛灯塔味业有限公司 | One kind clarification beverage of fig juice and preparation method thereof |
| CN107136369B (en) * | 2017-05-04 | 2020-08-11 | 青岛灯塔味业有限公司 | Clear fig juice beverage and preparation method thereof |
| CN109797182A (en) * | 2019-03-29 | 2019-05-24 | 江苏俊启生物科技股份有限公司 | A kind of preparation method of corn peptide |
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| CN103380935B (en) | 2014-05-14 |
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Effective date of registration: 20180703 Address after: 161200 Green Food Industrial Park, five street, Fuhe Town, Fuyu County, Qigihar, Heilongjiang Patentee after: Heilongjiang crane health wine industry Co., Ltd. Address before: 161200 No. 2 Minjiang Road, Nangang District, Harbin, Heilongjiang. Patentee before: Heilongjiang Dahuangchun Wine Industry Co., Ltd. |
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Address after: 161200 Green Food Industrial Park, five street, Fuhe Town, Fuyu County, Qigihar, Heilongjiang Patentee after: Heilongjiang Guli legend Health Wine Co., Ltd Address before: 161200 Green Food Industrial Park, five street, Fuhe Town, Fuyu County, Qigihar, Heilongjiang Patentee before: Heilongjiang crane health wine industry Co.,Ltd. |