CN103371101A - Acanthus ilicifolius plant tissue culture medium and preparation method thereof - Google Patents
Acanthus ilicifolius plant tissue culture medium and preparation method thereof Download PDFInfo
- Publication number
- CN103371101A CN103371101A CN 201210123957 CN201210123957A CN103371101A CN 103371101 A CN103371101 A CN 103371101A CN 201210123957 CN201210123957 CN 201210123957 CN 201210123957 A CN201210123957 A CN 201210123957A CN 103371101 A CN103371101 A CN 103371101A
- Authority
- CN
- China
- Prior art keywords
- naa
- proline
- agar powder
- sucrose
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to an acanthus ilicifolius plant tissue culture medium and a preparation method thereof. A differential medium is formed by dissolving BA (Butyl Acrylate), NAA (N Aceytl Aspartate), L-proline, L-glutamine, casein hydrolysate, cane sugar and agar powder in MS. A root medium is formed by dissolving NAA, L-proline, L-glutamine, casein hydrolysate, cane sugar and agar powder in the MS. The acanthus ilicifolius plant tissue culture medium provided by the invention has the advantages of fast breeding speed, strong growth vigor of plants, good stress resistance and the like.
Description
Technical field
The present invention relates to a kind of plant culture and preparation method.
Background technology
At present, Lao Shu le is extensively cultivated as the mangrove Silvicultural.In actual production, Lao Shu le generally adopts the seminal propagation mode, although the seminal propagation technology is extensively used, still exists many deficiencies, for example wastes time and energy, and planting percent is not high, thereby causes large-scale production difficult, can not need by satisfying the market.Traditional seminal propagation mode can not satisfy the needs of large-scale production, demand that can not satisfying the market.
Summary of the invention
The object of the present invention is to provide a kind of not only reproduction speed fast, the plant growing way is strong, and cost is low, is fit to Lao Shu le plant tissue culture media and the preparation method of large-scale production.
A kind of Lao Shu le plant tissue culture media that the present invention proposes, comprise differential medium and root media, above-mentioned three kinds of medium are all take MS as minimal medium, differential medium is to be the benzyl aminoadenine by BA, NAA is the a-methyl α-naphthyl acetate, L-PROLINE, Glu, caseinhydrolysate, sucrose and agar powder are dissolved in and form among the minimal medium MS, the consumption of its each component: MS is 1L, BA is 1mg, NAA is 0.05mg, L-PROLINE is 500MG, Glu is 500MG, caseinhydrolysate is 200MG, sucrose is 3000mg, and agar powder is 5500mg.
The preparation of described differential medium: by the said ratio raw materials weighing, first agar powder is added in the pot that distilled water is housed and boil, distilled water adds by 4/5 consumption, then add MS, L-PROLINE, Glu, caseinhydrolysate, BA, NAA in the pot, wait the rear adding sucrose that seethes with excitement, sucrose dissolves rear constant volume, adjust pH value to 5.8, then liquid medium behind the constant volume is packed into and carry out autoclaving in the container, wherein sterilising temp is 121～126 ℃, pressure is 0.1～0.14kPa, cools off at last below 10 ℃.
Described root media is that a-methyl α-naphthyl acetate, L-PROLINE, Glu, caseinhydrolysate, sucrose and agar powder are dissolved among the minimal medium MS and form by NAA, the consumption of its each component: MS is 1L, NAA is 0.2mg, L-PROLINE is that 500MG, Glu are that 500MG, caseinhydrolysate are that 200MG, sucrose are 3000mg, and agar powder is 550mg.
The preparation of described root media: by the said ratio raw materials weighing, first agar powder is added in the pot that distilled water is housed and boil, distilled water adds by 4/5 consumption, then add MS, L-PROLINE, Glu, caseinhydrolysate, NAA in the pot, wait the rear adding sucrose that seethes with excitement, sucrose dissolves rear constant volume, adjust pH value to 5.8, then liquid medium behind the constant volume is packed into and carry out autoclaving in the container, wherein sterilising temp is 121～126 ℃, pressure is 0.1～0.14kPa, cools off at last below 10 ℃.
Above-mentioned two kinds of medium are that differential medium, root media are prepared respectively, and support the use.
The present invention compared with prior art has following advantage: 1, shorten cultivation cycle, reproduction speed is fast; 2, rooting rate is high, and the plant growing way is strong, good stress resistance; 3, cost is low, is fit to large-scale production.
Description of drawings the present invention is without accompanying drawing.
Embodiment embodiment 1, take the preparation 1L differential medium as example, the MS of weighing 1L, the BA of 1mg, 0.2mg NAA, the L-PROLINE of 500mg, the Glu of 500mg, the caseinhydrolysate of 200mg, the sucrose of 3000mg, the agar powder of 5500mg, first agar powder add in the pot that 0.8L distilled water is housed and boil, and then add MS in the pot, L-PROLINE, Glu, caseinhydrolysate, BA, NAA, wait the rear adding sucrose that seethes with excitement, sucrose dissolves rear constant volume, and constant volume is 1L, adjusts pH value to 5.8, then liquid medium behind the constant volume is packed into and carry out autoclaving in the container, wherein sterilising temp is 121 ℃, and pressure is 0.1kPa, is cooled to 1 ℃.
Embodiment 2, take the preparation 10L differential medium as example, the MS of weighing 10L, the BA of 10mg, the NAA of 2mg, the L-PROLINE of 5000mg, the Glu of 5000mg, the caseinhydrolysate of 2000mg, the sucrose of 30000mg, the agar powder of 55000mg, first agar powder add in the pot that 8L distilled water is housed and boil, and then add MS in the pot, L-PROLINE, Glu, caseinhydrolysate, BA, NAA, wait the rear adding sucrose that seethes with excitement, sucrose dissolves rear constant volume, and constant volume is 10L, adjusts pH value to 5.8, then liquid medium behind the constant volume is packed into and carry out autoclaving in the container, sterilising temp is 126 ℃, and pressure is 0.14kPa, is cooled at last 1 ℃.
Through test, during Lao Shu le tissue was cultivated, the cultivation cycle of the differential medium of preparation was 15～25 days as stated above, has greatly shortened cultivation cycle, and Cell Differentiation improves 5～8 times.
Embodiment 3, take the preparation 1L root media as example: the MS of weighing 1L, 0.2mg NAA, the L-PROLINE of 500mg, the Glu of 500mg, the caseinhydrolysate of 200mg, the sucrose of 4000mg, the agar powder of 550mg adds agar powder in the pot that 0.8L distilled water is housed first and boils, and then adds MS in the pot, L-PROLINE, Glu, caseinhydrolysate, NAA, wait the rear adding sucrose that seethes with excitement, sucrose dissolves rear constant volume, and constant volume is 1L, adjusts pH value to 5.8, then liquid medium behind the constant volume is packed into and carry out autoclaving in the container, sterilising temp is 121 ℃, and pressure is 0.1kPa, is cooled at last 1 ℃.
Embodiment 4, take the preparation 10L root media as example: the MS of weighing 10L, the NAA of 2mg, the L-PROLINE of 5000mg, the Glu of 5000mg, the caseinhydrolysate of 2000mg, the sucrose of 40000mg, the agar powder of 5500mg adds agar powder in the pot that 8L distilled water is housed first and boils, and then adds MS in the pot, L-PROLINE, Glu, caseinhydrolysate, NAA, wait the rear adding sucrose that seethes with excitement, sucrose dissolves rear constant volume, and constant volume is 1L, adjusts pH value to 5.8, then liquid medium behind the constant volume is packed into and carry out autoclaving in the container, sterilising temp is 126 ℃, and pressure is 0.14kPa, is cooled at last 10 ℃.
During Lao Shu le tissue was cultivated, the root media of preparation can improve rooting rate greatly as stated above, through test, inoculate after 40 days rooting rate more than 90, and every offspring can be taken root about 5.
Claims (1)
1. old mouse le plant tissue culture media, it is characterized in that: comprise differential medium and root media, above-mentioned two kinds of medium are all take MS as minimal medium, differential medium is to be the benzyl aminoadenine by BA, NAA is the a-methyl α-naphthyl acetate, L-PROLINE, Glu, caseinhydrolysate, sucrose and agar powder are dissolved in and form among the minimal medium MS, the consumption of its each component: MS is 1L, BA is 1mg, NAA is 0.05mg, L-PROLINE is 500MG, Glu is 500MG, caseinhydrolysate is 200MG, sucrose is 3000mg, and agar powder is 5500mg; Root media is that a-methyl α-naphthyl acetate, L-PROLINE, Glu, caseinhydrolysate, sucrose and agar powder are dissolved among the minimal medium MS and form by NAA, the consumption of its each component: MS is 1L, NAA is 0.2mg, L-PROLINE is that 500MG, Glu are that 500MG, caseinhydrolysate are that 200MG, sucrose are 3000mg, and agar powder is 550mg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210123957 CN103371101A (en) | 2012-04-25 | 2012-04-25 | Acanthus ilicifolius plant tissue culture medium and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201210123957 CN103371101A (en) | 2012-04-25 | 2012-04-25 | Acanthus ilicifolius plant tissue culture medium and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103371101A true CN103371101A (en) | 2013-10-30 |
Family
ID=49457426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201210123957 Pending CN103371101A (en) | 2012-04-25 | 2012-04-25 | Acanthus ilicifolius plant tissue culture medium and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103371101A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105454043A (en) * | 2015-03-09 | 2016-04-06 | 佛山市农业科学研究所 | Tissue culture virus elimination and rapid propagation method of Carallia brachiata |
-
2012
- 2012-04-25 CN CN 201210123957 patent/CN103371101A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105454043A (en) * | 2015-03-09 | 2016-04-06 | 佛山市农业科学研究所 | Tissue culture virus elimination and rapid propagation method of Carallia brachiata |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102119655B (en) | Natural light rapid breeding method for dendrobium officinale | |
| CN102845311A (en) | Method for preparing phalaenopsis plant tissue medium | |
| CN105010109A (en) | Method for water planting grosvenor momordica | |
| CN101647391B (en) | Culture method of oriental lily test tube bulbs | |
| CN102318559A (en) | Culture medium composition for rapid rooting of blueberry tissue culture seedling outside test tube and preparation method | |
| CN102783415A (en) | Method for conservation in vitro of cassava germplasm resources with stability and high efficiency | |
| CN104823701A (en) | Selenium-enriched morchella submerged fermentation process | |
| CN102888379A (en) | Method for establishing fraxinus mandshurica suspension culture system | |
| CN104429849B (en) | A kind of new method for water planting blueberry | |
| CN103039333A (en) | Plant cultivation matrix | |
| CN103098695A (en) | Cultivation method of zostera marina high-quality seedling plants | |
| CN102864185B (en) | Method for producing citric acid by fermenting sorghum powder | |
| CN101984787B (en) | Method for strengthening tissue culture seedlings of rhododendron lapponicum | |
| CN105028192A (en) | Culture medium series for rapidly breeding dendrobium nobile seedlings and tissue culture method | |
| CN105418207A (en) | Low-cost and environmental-protection plant cultivation matrix | |
| CN103371101A (en) | Acanthus ilicifolius plant tissue culture medium and preparation method thereof | |
| CN104450849A (en) | Method for forcing dunaliella tertiolecta to accumulate beta-carotene | |
| CN103468633B (en) | Inducing method for improving cell synchronization of tendril-leaved fritillary bulbs | |
| CN103766100A (en) | High-yield de dendrocalamus latiflorus culturing method | |
| CN102845307A (en) | Method for acquiring rubber tree embryoid by anther callus subculture | |
| CN104973971A (en) | Dracaena sanderiana soilless culture nutrient formula and application thereof | |
| CN104255453B (en) | A kind of store method of stevia plantlet in vitro | |
| CN104137776B (en) | Blueberry tissue cultured seedling liquid rooting method and special culturing container | |
| CN103319230A (en) | Soilless culture composite fertilizer for potted rosa chinensis minima | |
| CN105210883A (en) | The medium of a kind of tuber crops and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20131030 |
|
| DD01 | Delivery of document by public notice |
Addressee: Wang Qingbiao Document name: Notification that Application Deemed to be Withdrawn |