CN103357064A - Magnetic polylactic acid tissue engineering bracket - Google Patents
Magnetic polylactic acid tissue engineering bracket Download PDFInfo
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- CN103357064A CN103357064A CN2013102318870A CN201310231887A CN103357064A CN 103357064 A CN103357064 A CN 103357064A CN 2013102318870 A CN2013102318870 A CN 2013102318870A CN 201310231887 A CN201310231887 A CN 201310231887A CN 103357064 A CN103357064 A CN 103357064A
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- polylactic acid
- tissue engineering
- engineering bracket
- magnetic
- poly
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Abstract
The invention discloses a magnetic polylactic acid tissue engineering bracket. A magnet tissue engineering bracket suitable for targeting of a magnetic targeting medicine feeding system is produced by adding Fe3O4 into a polylactic acid tissue engineering bracket and is a multiporous material containing the Fe3O4; the polylactic acid comprises poly-L-lactic acid, poly-R-lactic acid or poly-dl-lactic acid. The toxic action of medicines to the whole body is reduced, a complicated step of the medicine feeding process is reduced, and the magnetic polylactic acid tissue engineering bracket can be used for other treatment measures taking magnetism as targeting.
Description
Technical field
The present invention relates to a kind of magnetic polylactic acid tissue engineering bracket.
Background technology
The primary and foremost purpose of tissue engineering technique development be applied to clinical, with treatment because tissue loss or disease that the organ failure was produced.Traditional Therapeutic Method comprises organ transplantation, surgical reconstruction, use machinery and regular injection of medicine etc., and these methods are not only expensive but also usually can not effectively reach therapeutic purposes.
Along with the development of life sciences and physics, chemistry, material subject, tissue engineering is arisen at the historic moment, and this has just fundamentally solved dysfunction due to tissue and the organ defect or the treatment problem of forfeiture.Its basic skills is the high concentration histiocyte with In vitro culture, be adsorbed in after the amplification a kind of biocompatibility good, and can be by human body progressively on the extracellular matrix of degraded and absorbed.This material can be the three dimensions that cell provides existence, is conducive to cell and obtains enough nutrient substance, carries out gas exchange, gets rid of waste material, makes cell by the three-dimensional rack growth of prefabricated form.Then with this cell biological composite body implanting to human body disease damage position, at biological support progressively in the degraded and absorbed process, the cell of plantation continues the hypertrophy breeding, has formed new respective organization and organ with its original specific function and form, reaches the purpose of repairing wound and Reconstruction of The Function.
Tissue engineering bracket is the engineered the most basic framework of organizing, and it not only provides the support structure effect for specific cell, but also plays template action, guide tissue regeneration and control organizational structure.Polylactic acid degradable, nontoxic, easy processing are the tissue engineering bracket materials of commonly using.
The magnetic target drug-supplying system reduces medicine to the toxic action of whole body owing to can treat more targetedly disease, and becomes the focus of research.But the driving force that traditional magnetic target drug-supplying system externally-applied magnetic field commonly used is assembled as medicine is so that the administration process steps is loaded down with trivial details.
Summary of the invention
The objective of the invention is provides a kind of magnetic polylactic acid tissue engineering bracket for overcoming above-mentioned the deficiencies in the prior art.The present invention is with Fe
3O
4Add the polylactic acid tissue engineering bracket, the magnetic tissue engineering bracket that can be used for magnetic target drug-supplying system targeting of preparation tool.
For achieving the above object, the present invention adopts following technical proposals:
A kind of magnetic polylactic acid tissue engineering bracket, it is for containing Fe in polylactic acid
3O
4Porous material, described polylactic acid comprises Poly-L-lactic acid, poly-L-lactic acid or poly-racemic lactic acid.
Described Fe
3O
4Mass content is 0.1～80%.
Described Fe
3O
4Particle diameter is 1～10
7Between the nm, described porosity is 0.01～99.9%, and the aperture is 1～10
7Nm.
The preparation method of above-mentioned magnetic polylactic acid tissue engineering bracket comprises solvent cast-granule leaching, film material lamination, fiber combination, melt molding, extrudes leaching, emulsion lyophilization, heat bring out be separated, supercritical fluid technology, supercritical liq-leaching, 3 D-printing (leaching) or melt extrude molding methods etc.
Beneficial effect of the present invention:
(1) reduces medicine to the toxic action of whole body.
(2) the loaded down with trivial details step of minimizing administration process.
(3) can be used for other with the treatment means of magnetic as targeting.
Description of drawings
Fig. 1 is the stereoscan photograph of polylactic acid magnetic tissue engineering bracket;
Fig. 2 contains 20%Fe
3O
4The hysteresis curve figure of polylactic acid magnetic tissue engineering bracket (G2);
Fig. 3 is the cytotoxicity with KB cell detection material.
The specific embodiment
The present invention will be further elaborated below in conjunction with drawings and Examples, should be noted that following explanation only is in order to explain the present invention, its content not to be limited.
Embodiment 1
The poly-racemic lactic acid of 2g is dissolved in the 20ml dichloromethane, with the Fe of 0.15g particle diameter less than 50nm
3O
4Powder and 19g particle diameter are to add in the poly-racemic lactic acid solution of fully dissolving behind the NaCl mix homogeneously of 300-400 micron, stir, and pour mould into.Allow solvent fully naturally volatilize, behind the dry 6h of normal-temperature vacuum, add the abundant leaching of distilled water, so that porogen NaCl stripping makes and contains Fe
3O
4Polylactic acid magnetic tissue engineering bracket.
The 10g Poly-L-lactic acid is dissolved in the 100ml chloroform, with the Fe of 2g particle diameter less than 5 microns
3O
4Powder and 20g particle diameter are to add in the poly-left lactic acid solution of fully dissolving behind the NaCl mix homogeneously of 0-100 micron, stir, and mixed liquor is cast on the culture dish, allow the chloroform film forming of naturally volatilizing under the room temperature in fume hood.Culture dish was put into the vacuum drying oven vacuum drying 48 hours.Then film is taken out, pulverize with the stone roller alms bowl, utilize mould, material is hot-forming with 170 ℃, the pressure of 15MPa on vulcanizer.The demoulding drops to room temperature etc. material temperature, adds the abundant leaching of distilled water, so that porogen NaCl stripping makes and contains Fe
3O
4Polylactic acid magnetic tissue engineering bracket.
The poly-L-lactic acid of 5g is dissolved in the 40ml dichloromethane, with the Fe of 0.2g particle diameter less than 100nm
3O
4Powder adds in the poly-L-lactic acid solution of fully dissolving, stirs, and behind the liquid nitrogen flash freezer, puts into vacuum freeze drier, so that solvent fully volatilizees, makes and contains Fe
3O
4Polycaprolactone magnetic tissue engineering bracket.
Embodiment 4
To gather racemic lactic acid and be dissolved in dichloromethane (mass volume ratio g/ml is 1:10), with an amount of Fe
3O
4Powder (poly-racemic lactic acid and Fe
3O
4It is 1:0 that the powder quality ratio is respectively G0; G1 is 0.9:0.1; G2 is 0.8:0.2; G4 is 0.6:0.4) and particle diameter to be 200-300 micron quality be poly-racemic lactic acid and Fe
3O
4Add in the poly-racemic lactic acid solution of fully dissolving behind the NaCl mix homogeneously that the powder quality sum is 9 times, stir, pour mould into.Volatilization is after 7 days fully naturally to allow solvent, and the abundant leaching of adding distilled water is so that porogen NaCl stripping makes and contains 0%(G0), 10%(G1), 20%(G2) and 40%(G3) Fe
3O
4Poly-racemic lactic acid magnetic tissue engineering bracket.
Fig. 1 is the stereoscan photograph of polylactic acid magnetic tissue engineering bracket, and Fig. 1 a is photo corresponding to G0, and Fig. 1 b is photo corresponding to G1, and Fig. 1 c is photo corresponding to G2, and Fig. 1 d is photo corresponding to G3.
Fig. 2 contains 20%Fe
3O
4The hysteresis curve figure of polylactic acid magnetic tissue engineering bracket (G2).The result shows: its saturation magnetization Ms is 15.87emu/g, and remanent magnetism Mr is 1.73emu/g, and coercivity H is 142Oe, has good magnetic.
Fig. 3 be with the cytotoxicity of KB cell detection material (take without the culture fluid of any processing as control group).The cuboid small pieces of 2.5mm*10mm*10mm are immersed in the 1ml culture fluid, extract soak every day and add new culture fluid, so repeatedly, extract the soak of 1 day, 2 days, 3 days, 4 days, 5 days, 6 days and 7 days.The 100 microlitre culture fluid that will contain the 10000KB cell add each aperture of 96 porose discs, second day adds 100 microlitre soaks or 100 microlitre fresh mediums (control group), cultivate after 2 days, adopt mtt assay to detect cytoactive, Fig. 3 a, 3b, 3c, 3d, 3e, 3f, 3g are followed successively by 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days test result, and the result shows that polylactic acid magnetic tissue engineering bracket does not have toxicity to cell.
Although above-mentionedly by reference to the accompanying drawings the specific embodiment of the present invention is described; but be not limiting the scope of the invention; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various modifications that creative work can make or distortion still in protection scope of the present invention.
Claims (4)
1. a magnetic polylactic acid tissue engineering bracket is characterized in that, it is for containing Fe in polylactic acid
3O
4Porous material, described polylactic acid comprises Poly-L-lactic acid, poly-L-lactic acid or poly-racemic lactic acid.
2. a kind of magnetic polylactic acid tissue engineering bracket according to claim 1 is characterized in that described Fe
3O
4Mass content is 0.1～80%.
3. a kind of magnetic polylactic acid tissue engineering bracket according to claim 1 is characterized in that described Fe
3O
4Particle diameter is 1～10
7Between the nm, described porosity is 0.01～99.9%, and the aperture is 1～10
7Nm.
4. the preparation method of the described a kind of magnetic polylactic acid tissue engineering bracket of above-mentioned each claim, it is characterized in that, concrete steps are: adopt solvent cast-granule leaching, film material lamination, fiber combination, melt molding, extrude leaching, emulsion lyophilization, heat bring out be separated, supercritical fluid technology, supercritical liq-leaching, 3 D-printing or melt extrude molding methods.
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| CN2013102318870A CN103357064A (en) | 2013-06-09 | 2013-06-09 | Magnetic polylactic acid tissue engineering bracket |
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| CN2013102318870A CN103357064A (en) | 2013-06-09 | 2013-06-09 | Magnetic polylactic acid tissue engineering bracket |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107670108A (en) * | 2017-09-27 | 2018-02-09 | 四川大学 | A kind of tissue engineering bracket polylactic acid porous material and preparation method thereof |
| CN108943700A (en) * | 2018-07-18 | 2018-12-07 | 中南大学 | A kind of preparation method of Poly L-lactic acid/ferroso-ferric oxide Composite Bone bracket |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011075516A2 (en) * | 2009-12-18 | 2011-06-23 | President And Fellows Of Harvard College | Active scaffolds for on-demand drug and cell delivery |
-
2013
- 2013-06-09 CN CN2013102318870A patent/CN103357064A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011075516A2 (en) * | 2009-12-18 | 2011-06-23 | President And Fellows Of Harvard College | Active scaffolds for on-demand drug and cell delivery |
Non-Patent Citations (2)
| Title |
|---|
| J. YANG ETAL.: ""Preparation of poly ε-caprolactone nanoparticles containing magnetite for magnetic drug carrier"", 《INTERNATIONAL JOURNAL OF PHARMACEUTICS》, vol. 324, no. 2, 23 June 2006 (2006-06-23), pages 185 - 190, XP025113192, DOI: doi:10.1016/j.ijpharm.2006.06.029 * |
| KYUNG-YON KIM ETAL.: ""Characterization and Preparation of PCL encapsulated Fe3O4 nanoparticle for targeted drug delivery system(Ⅱ)"", 《THEORIES AND APPLICATIONS OF CHEMICAL ENGINEERING》, vol. 8, no. 2, 28 February 2002 (2002-02-28), pages 5182 - 5185 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107670108A (en) * | 2017-09-27 | 2018-02-09 | 四川大学 | A kind of tissue engineering bracket polylactic acid porous material and preparation method thereof |
| CN108943700A (en) * | 2018-07-18 | 2018-12-07 | 中南大学 | A kind of preparation method of Poly L-lactic acid/ferroso-ferric oxide Composite Bone bracket |
| CN108943700B (en) * | 2018-07-18 | 2020-10-02 | 中南大学 | Preparation method of poly-L-lactic acid/ferroferric oxide composite bone scaffold |
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Application publication date: 20131023 |