CN103341205A - Magnetic chitosan tissue engineering scaffold - Google Patents
Magnetic chitosan tissue engineering scaffold Download PDFInfo
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- CN103341205A CN103341205A CN2013102318866A CN201310231886A CN103341205A CN 103341205 A CN103341205 A CN 103341205A CN 2013102318866 A CN2013102318866 A CN 2013102318866A CN 201310231886 A CN201310231886 A CN 201310231886A CN 103341205 A CN103341205 A CN 103341205A
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- tissue engineering
- magnetic
- chitosan
- engineering bracket
- leaching
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Abstract
The invention relates to a magnetic chitosan tissue engineering scaffold. The scaffold is a chitosan tissue engineering scaffold containing 0.1 to 80% by mass of Fe3O4 and is of a porous structure with porosity of 0.01 to 99.9% and a bore diameter of 1 to 107 nm. The magnetic chitosan tissue engineering scaffold provided by the invention can reduce poisoning effects of a drug on the whole body and cumbersome steps in the process of magnetic targeted drug delivery and is applicable to other treatment means using magnetism for targeting.
Description
Technical field
The present invention relates to a kind of chitosan magnetic tissue engineering bracket.
Background technology
The primary and foremost purpose of tissue engineering technique development be applied to clinical, with treatment because tissue loss or disease that the organ failure was produced.Traditional Therapeutic Method comprises organ transplantation, surgical reconstruction, use machinery and regularly injects medicine etc., and these methods are not only expensive but also can not reach therapeutic purposes effectively usually.
Along with the development of life sciences and physics, chemistry, material subject, tissue engineering is arisen at the historic moment, and this has just fundamentally solved dysfunction due to tissue and the organ defect or the treatment problem of forfeiture.Its basic skills is the high concentration histiocyte with In vitro culture, be adsorbed in after the amplification a kind of biocompatibility good, and can be by human body progressively on the extracellular matrix of degraded and absorbed.This material can be the three dimensions that cell provides existence, is conducive to cell and obtains enough nutrient substance, carries out gas exchange, gets rid of waste material, makes cell by the three-dimensional rack growth of prefabricated form.Then with the sick position of decreasing of this cell biological composite body implanting to human body, at biological support progressively in the degraded and absorbed process, the cell of plantation continues the hypertrophy breeding, has formed new respective organization and organ with its original specific function and form, reaches the purpose of repairing wound and rebuilding function.
Tissue engineering bracket is the engineered the most basic framework of organizing, and it not only provides the support structure effect for specific cell, but also plays template action, guide tissue regeneration and control organizational structure.Polycaprolactone degradable, nontoxic, easy processing are the tissue engineering bracket materials of using always.
The magnetic target drug-supplying system reduces medicine to the toxic action of whole body owing to can treat disease more targetedly, and becomes the focus of research.But the driving force that traditional magnetic target drug-supplying system externally-applied magnetic field commonly used is assembled as medicine makes that the administration process steps is loaded down with trivial details.
Summary of the invention
The objective of the invention is to overcome above-mentioned deficiency, and a kind of chitosan magnetic tissue engineering bracket is provided, obtain having the tissue engineering bracket that can be used for magnetic target drug-supplying system targeting of magnetic.
The technical scheme that the present invention takes is:
A kind of chitosan magnetic tissue engineering bracket, it is the Fe that contains mass percent 0.1-80%
3O
4The chitosan tissue engineering bracket.
Described chitosan magnetic tissue engineering bracket is porosity 0.01-99.9%, aperture 1-10
7The loose structure of nm.
Described Fe
3O
4, refer to that particle diameter is at 1-10
7Fe between the nm
3O
4
The processing method of described chitosan magnetic tissue engineering bracket, solvent cast-granule leaching, film material lamination, fiber combination, melt molding arranged, extrude leaching, emulsion lyophilization, heat bring out be separated, supercritical fluid technology, supercritical liq-leaching, 3 D-printing (leaching) or melt extrude methods such as molding.
Beneficial effect of the present invention:
(1) reduces medicine to the toxic action of whole body.
(2) the loaded down with trivial details step of minimizing administration process.
(3) can be used for other with the treatment means of magnetic as targeting.
Description of drawings
Fig. 1 is the stereoscan photograph of chitose magnetic sex organization engineering rack;
Fig. 2 contains 20%Fe
3O
4The hysteresis curve figure of chitose magnetic sex organization engineering rack (I2);
Fig. 3 is for the cell toxicity test of KB cell detection material figure as a result.
The specific embodiment
Further specify the present invention below in conjunction with embodiment, but these embodiment are not meant to and limit the scope of the invention.
Embodiment 1
The 2g chitosan is dissolved in the 2wt% aqueous acetic acid, with the Fe of 1g particle diameter less than 50nm
3O
4Powder adds in the chitosan solution of fully dissolving, stirs, and pours mould into.After the lyophilization 4 days, use in the NaOH weak solution and acetic acid, make and contain Fe
3O
4Chitose magnetic sex organization engineering rack.
The 5g chitosan is dissolved in the 4wt% aqueous acetic acid, with the Fe of 0.5g particle diameter less than 5 microns
3O
4The NaCl mix homogeneously of powder and 15g particle diameter 200-300 micron adds in the chitosan solution of fully dissolving, stirs, and behind the liquid nitrogen flash freezer, puts into vacuum freeze drier, makes solvent fully volatilize.With in the NaOH weak solution and behind the acetic acid, add the leaching repeatedly of a large amount of distilled water, make and contain Fe
3O
4Chitose magnetic sex organization engineering rack.
Embodiment 3
Chitosan is dissolved in the 2wt% aqueous acetic acid, with an amount of Fe
3O
4Powder (chitosan and Fe
3O
4It is 0.9:0.1 that the powder quality ratio is respectively I1; I2 is 0.8:0.2; I4 is 0.6:0.4) add in the chitosan solution of fully dissolving, stir, pour mould into.After-80 ° of C spend the night, put into freezer dryer.After the lyophilization 4 days, with in the NaOH weak solution and acetic acid, lyophilization again makes and contains 0%(I0), 10%(I1), 20%(I2) and 40%(I3) Fe
3O
4Chitose magnetic sex organization engineering rack.
Comparative Examples
Preparation method is with embodiment 3, and difference is chitosan and Fe
3O
4Powder quality compares 1:0; Make and contain 0%(I0) Fe
3O
4Chitose magnetic sex organization engineering rack.
Performance test:
Fig. 1 is the stereoscan photograph of chitose magnetic sex organization engineering rack.
Fig. 2 contains 20%Fe
3O
4The hysteresis curve figure of chitose magnetic sex organization engineering rack (I2).The result shows: its saturation magnetization Ms is 11.17emu/g, and remanent magnetism Mr is 1.1emu/g, and coercivity H is 145Oe, has good magnetic.
Fig. 3 is with the cytotoxicity of KB cell detection material (be control group with the culture fluid without any processing).The cuboid small pieces of 2.5mm * 10mm * 10mm are immersed in the 1ml culture fluid, extract soak every day and add new culture fluid, so repeatedly, extract the soak of 1d, 2d, 3d, 4d, 5d, 6d and 7d.The 100 microlitre culture fluid that will contain the 10000KB cell add each aperture of 96 porose discs, added 100 microlitre soaks or 100 microlitre fresh mediums (control group) in second day, cultivate after 2 days, adopt mtt assay to detect cytoactive, the result shows that chitose magnetic sex organization engineering rack does not have toxicity to cell.
Claims (4)
1. chitosan magnetic tissue engineering bracket is characterized in that containing the Fe of mass percent 0.1-80%
3O
4The chitosan tissue engineering bracket.
2. a kind of chitosan magnetic tissue engineering bracket according to claim 1 is characterized in that, described chitosan magnetic tissue engineering bracket is porosity 0.01-99.9%, aperture 1-10
7The loose structure of nm.
3. a kind of chitosan magnetic tissue engineering bracket according to claim 1 is characterized in that described Fe
3O
4Refer to that particle diameter is at 1-10
7Fe between the nm
3O
4
4. the processing method of the described a kind of chitosan magnetic tissue engineering bracket of claim 1, it is characterized in that, for solvent cast-granule leaching, film material lamination, fiber combination, melt molding, extrude leaching, emulsion lyophilization, heat bring out be separated, supercritical fluid technology, supercritical liq-leaching, 3 D-printing leaching or melt extrude method of molding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102318866A CN103341205A (en) | 2013-06-09 | 2013-06-09 | Magnetic chitosan tissue engineering scaffold |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013102318866A CN103341205A (en) | 2013-06-09 | 2013-06-09 | Magnetic chitosan tissue engineering scaffold |
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| Publication Number | Publication Date |
|---|---|
| CN103341205A true CN103341205A (en) | 2013-10-09 |
Family
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| CN2013102318866A Pending CN103341205A (en) | 2013-06-09 | 2013-06-09 | Magnetic chitosan tissue engineering scaffold |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115400263A (en) * | 2022-08-18 | 2022-11-29 | 东华大学 | Preparation method of magnetoelectric fiber, magnetoelectric fiber and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1951498A (en) * | 2006-08-07 | 2007-04-25 | 中国科学院广州化学研究所 | A fibroid magnetic medicament and preparation method thereof |
| WO2011075516A2 (en) * | 2009-12-18 | 2011-06-23 | President And Fellows Of Harvard College | Active scaffolds for on-demand drug and cell delivery |
-
2013
- 2013-06-09 CN CN2013102318866A patent/CN103341205A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1951498A (en) * | 2006-08-07 | 2007-04-25 | 中国科学院广州化学研究所 | A fibroid magnetic medicament and preparation method thereof |
| WO2011075516A2 (en) * | 2009-12-18 | 2011-06-23 | President And Fellows Of Harvard College | Active scaffolds for on-demand drug and cell delivery |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115400263A (en) * | 2022-08-18 | 2022-11-29 | 东华大学 | Preparation method of magnetoelectric fiber, magnetoelectric fiber and application thereof |
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Application publication date: 20131009 |