CN105381826A - Preparation method of microfluidic three-dimensional gel chip model - Google Patents
Preparation method of microfluidic three-dimensional gel chip model Download PDFInfo
- Publication number
- CN105381826A CN105381826A CN201510826081.5A CN201510826081A CN105381826A CN 105381826 A CN105381826 A CN 105381826A CN 201510826081 A CN201510826081 A CN 201510826081A CN 105381826 A CN105381826 A CN 105381826A
- Authority
- CN
- China
- Prior art keywords
- mould
- gel
- preparation
- dimensional
- micro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a preparation method of a microfluidic three-dimensional gel chip model and belongs to the field of biological micro-processing. A preparation process comprises the following steps: (1) preparing a micro-fiber columnar mold; (2) embedding the prepared mold into a three-dimensional biological gel; (3) dissolving the mold and constructing a micro-channel in the three-dimensional biological gel so as to form the microfluidic three-dimensional gel chip model. A method for constructing a microfluidic chip in the three-dimensional biological gel is realized and the method has a simple preparation process; the size and space structure of the formed micro-channel can be adjusted according to actual requirements. The microfluidic three-dimensional gel chip model can be used for research in the fields of tissue microenvironments, vascular tissue engineering, 3D drug delivery and screening and the like.
Description
Technical field
The present invention relates to a kind of preparation method of micro-fluidic three dimensional gel chip model, belong to biological micro-processing technology field.
Background technology
In the scientific experiment such as biology, chemistry, material, often need to operate fluid.And no matter the hydrogel with MCA is preparing functional material, still simulating in vitro and rebuilding in blood vessel tissue and can play very important effect.By at the suitable MCA of hydrogel internal production, thus vascular system in organism can be simulated.In addition, by changing the component of hydrogel, the application such as microchannel screening and biochip test can also be realized.
The construction method of current three-dimensional channel mainly uses photoetching technique, 3D printing technique etc.These technology preparation method is complicated, cost is higher, and the network shape being difficult to realize labyrinth builds.The present invention uses biogel as substrate, constructs the micro-fluidic chip model of a similar blood vessel access therein.This invention has extremely important meaning for the research in the fields such as tissue microenvironment, intravascular tissue engineering, 3D drug delivery and screening.
Summary of the invention
The present invention aims to provide the preparation method of a kind of economy micro-fluidic three dimensional gel chip model fast.
The invention provides a kind of preparation method of micro-fluidic three dimensional gel chip model, at the micro-fluidic chip of three dimensional biological gel internal build blood vessel access type; Comprise the steps:
The first step: the preparation of microfibre column " mould ":
Sodium alginate soln is injected ionic calcium soln, gel reaction 5 ~ 60 minutes, form calcium alginate microfibre column " mould ", by the diameter control of " mould " within the scope of 50-2000 μm;
Second step: " mould " is embedded in three dimensional biological gel:
" mould " that prepare is placed in liquid biogel mixture, after gel sets, forms the three dimensional gel being embedded with " mould "; I.e. solidifiable that gel at room temperature cools 20 ~ 25 minutes.
3rd step: use lysate to dissolve " mould ", form microchannel in three dimensional biological gel, build micro-fluidic three dimensional gel chip model.
Further, in the described first step, the mass concentration of sodium alginate is 0.5-3%, and described calcium ion concentration is 45 ~ 200mmol/L.
Further, described ionic calcium soln is calcium chloride or calcium nitrate solution.
Further, in the described first step, sodium alginate soln injects ionic calcium soln by syringe, and syringe needle specification is 14G-34G, and charge velocity is 20-200 μ L/s.
Further, in described second step, biogel comprises: any one in agar, gelatin, collagen, agar-collagen, agar-gelatin, collagen-gelatin, and the mass concentration of described biogel is 5-20mg/ml.
Further, in described 3rd step, the lysate used is sodium citrate solution, and concentration is 10 ~ 200mmol/L, and dissolution time is 0.5 ~ 5 hour.
Of the present invention
beneficial effect:
The present invention can be quick, economical, prepare micro-fluidic three dimensional gel chip easily, and form microchannel size and Space structural adjustment method is simple, easy to operate.This invention provides a kind of method of structure three-dimensional chip newly to the structure of tissue microenvironment, intravascular tissue engineering Zhong Lei vascular tissue and the field such as 3D drug delivery and screening.
Accompanying drawing explanation
Fig. 1 is the preparation process schematic diagram of micro-fluidic three dimensional gel chip model.
Fig. 2 is binary channels shaping schematic view.
Fig. 3 is multichannel shaping schematic view.
Detailed description of the invention
Further illustrate the present invention below by embodiment, but be not limited to following examples.
Embodiment 1:
Agar embedding calcium alginate column " mould " preparation can pour into microfluid three dimensional biological gel single channel.
Calcium alginate column " mould " preparation in use Ca
2+solution is provided by calcium chloride solution.
(1) preparation of microfibre column " mould ": use model is G31 syringe, with the speed of 100 μ L/s be in the sodium alginate soln calcium chloride solution that at the uniform velocity implantation concentration is 90mmol/L of 1% by mass concentration, gel reaction 15 minutes, forms calcium alginate column " mould ".
(2) " mould " is embedded in three-dimensional agar gel: preparation mass concentration is the agar 10ml of 10mg/mL.High-pressure sterilizing pot 121 DEG C of high temperature melt sterilizings.Take out immediately after sterilizing, absorption 5ml agar is evenly flat is applied to culture dish.After cooling, single calcium alginate " mould " is placed on gel, and gel whole story end reservedly inject entrance.Reuse 5ml agar solution and slowly embed " mould " structure.Room temperature cools 20 minutes, forms calcium alginate column " mould " single-pass configuration embedded by agar gel.
(3) " mould " dissolves: above-mentioned " mould " single-pass configuration being immersed concentration is soak 4 hours in the sodium citrate solution of 35mmol/L.
Observation duct is transparent, shows that column " mould " is dissolved completely, and single channel is formed.As shown in figure ip.Inject Yin's red at injection port place, observe single pass circulation further, experiment shows, single channel circulation is good.
Embodiment 2:
Agar embedding calcium alginate column " mould " preparation can pour into microfluid three dimensional biological gel binary channels.
Calcium alginate column " mould " preparation in use Ca
2+solution is provided by calcium nitrate solution.
(1) preparation of microfibre column " mould ": use model is G22 syringe, be that the sodium alginate soln of 1.5% is with in the speed of the 140 μ L/s calcium nitrate solution that at the uniform velocity implantation concentration is 60mmol/L by mass concentration, gel reaction 40 minutes, forms calcium alginate column " mould ".
(2) " mould " is embedded in three-dimensional agar gel: preparation mass concentration is the agar 10ml of 15mg/mL.High-pressure sterilizing pot 121 DEG C of high temperature melt sterilizings.Take out immediately after sterilizing, absorption 5ml agar is evenly flat is applied to culture dish.After cooling, two calcium alginates " mould " intersection is placed on gel, and injects entrance gel end at the whole story is reserved.Reuse 5ml agar solution and slowly embed " mould " structure.Room temperature cools 30 minutes, forms calcium alginate column " mould " channel structure embedded by agar gel.
(3) " mould " dissolves: above-mentioned " mould " channel structure being immersed concentration is soak 2.5 hours in the sodium citrate solution of 100mmol/L.
Observation duct is transparent, shows that column " mould " is dissolved completely, and channel structure is formed.As shown in Figure 2.Inject Yin's red at injection port place, observe twin-channel circulation further, experiment shows, binary channels circulation is good.
Embodiment 3:
Gelatin embedding calcium alginate column " mould " preparation can pour into microfluid three dimensional biological gel single channel.
Calcium alginate column " mould " preparation in use Ca
2+solution is provided by calcium chloride solution.
(1) preparation of microfibre column " mould ": use model is G33 syringe, with the speed of 80 μ L/s be in the sodium alginate soln calcium chloride solution that at the uniform velocity implantation concentration is 120mmol/L of 1.2% by mass concentration, gel reaction 35 minutes, forms calcium alginate column " mould ".
(2) " mould " is embedded in three-dimensional isinglass gel: preparation mass concentration is the gelatin 10ml of 12mg/mL.High-pressure sterilizing pot 121 DEG C of high temperature melt sterilizings.Take out immediately after sterilizing, absorption 5ml gelatin is evenly flat is applied to culture dish.After cooling, single calcium alginate " mould " is placed on gel, and gel whole story end reservedly inject entrance.Reuse 5ml gelatin solution and slowly embed " mould " structure.Room temperature cools 20 minutes, forms calcium alginate column " mould " single-pass configuration embedded by gelatin gel.
(3) " mould " dissolves: above-mentioned " mould " single-pass configuration being immersed concentration is soak 3 hours in the sodium citrate solution of 80mmol/L.
Observation duct is transparent, shows that column " mould " is dissolved completely, and single channel is formed.As shown in figure ip.Inject Yin's red at injection port place, observe single pass circulation further, experiment shows, single channel circulation is good.
Embodiment 4:
Mouse tail I-type collagen gel embedding calcium alginate column " mould " preparation can pour into microfluid three dimensional biological gel single channel.
Calcium alginate column " mould " preparation in use Ca
2+solution is provided by calcium chloride solution.
(1) preparation of microfibre column " mould ": use model is G31 syringe, with the speed of 90 μ L/s be in the sodium alginate soln calcium chloride solution that at the uniform velocity implantation concentration is 130mmol/L of 0.8% by mass concentration, gel reaction 45 minutes, forms calcium alginate column " mould ".
(2) " mould " is embedded in three-dimensional collagen gel: preparation 8mg/ml mouse tail I-type collagen 10ml, regulates pH to be 7.0 under ice bath.Absorption 5ml collagen solution is evenly flat is applied to culture dish.In 37 DEG C of water-baths, single calcium alginate " mould " is placed on gel, and gel whole story end reservedly inject entrance.Reuse 5ml collagen solution and slowly embed " mould " structure.37 DEG C of water-baths, after 25 minutes, form calcium alginate column " mould " single-pass configuration embedded by collagen gel.
(3) " mould " dissolves: above-mentioned " mould " single-pass configuration being immersed concentration is soak 2 hours in the sodium citrate solution of 150mmol/L.
Observation duct is transparent, shows that column " mould " is dissolved completely, and single channel is formed.As shown in figure ip.Inject Yin's red at injection port place, observe single pass circulation further, experiment shows, single channel circulation is good.
Embodiment 5:
Agar-mouse tail I-type collagen mixed gel embedding calcium alginate column " mould " preparation can pour into microfluid three dimensional gel multichannel.
Calcium alginate column " mould " preparation in use Ca
2+solution is provided by calcium chloride solution.
(1) preparation of microfibre column " mould ": use model is G27 syringe, by mass concentration be 2% sodium alginate soln with the speed of 120 μ L/s at the uniform velocity implantation concentration in 150mmol/L calcium chloride solution, gel reaction 35 minutes, forms calcium alginate column " mould ".
(2) " mould " is embedded in agar-collagen gel: preparation mass concentration is the agar 10ml of 15mg/mL.High-pressure sterilizing pot high temperature 121 DEG C thawing.Preparation 15mg/ml mouse tail I-type collagen 10ml, regulating pH to be 7.0 under ice bath, is agar-collagen mixed solution with agar equal-volume mixed preparing.Absorption 5ml agar-collagen mixed gel dissolution homogeneity is flat is applied to culture dish.After cooling, many calcium alginates " mould " are built scalariform network structure and are placed on gel, and gel whole story end reservedly inject entrance.Reuse 5ml agar-collagen solution and slowly embed " mould " structure.Room temperature cools 50 minutes, forms calcium alginate column " mould " multi-channel structure embedded by agar-collagen gel.
(3) " mould " dissolves: above-mentioned " mould " multi-channel structure being immersed concentration is soak 4 hours in the sodium citrate solution of 60mmol/L.
Observation duct is transparent, shows that column " mould " is dissolved completely, and multi-channel structure is formed.As shown in Figure 3.Inject Yin's red at injection port place, observe multichannel circulation further, experiment shows, multi-passage circulating is good.
Embodiment 6:
Agar-gelatin mixed gel embedding calcium alginate column " mould " preparation can pour into microfluid three dimensional gel single channel.
Calcium alginate column " mould " preparation in use Ca
2+solution is provided by calcium chloride solution.
(1) preparation of microfibre column " mould ": use model is G20 syringe, by mass concentration be 2% sodium alginate soln with the speed of 150 μ L/s at the uniform velocity implantation concentration in 160mmol/L calcium chloride solution, gel reaction 50 minutes, forms calcium alginate column " mould ".
(2) " mould " is embedded in agar-gelatin gel: preparation mass concentration is the agar 10ml of 13mg/mL.High-pressure sterilizing pot high temperature 121 DEG C thawing.Preparation 13mg/ml gelatin solution 10ml is agar-gelatin mixed solution with agar equal-volume mixed preparing.Absorption 5ml agar-gelatin mixed gel dissolution homogeneity is flat is applied to culture dish.After cooling, single calcium alginate " mould " is placed on gel, and gel whole story end reservedly inject entrance.Reuse 5ml agar-gelatin solution and slowly embed " mould " structure.Room temperature cools 35 minutes, is formed by calcium alginate column " mould " single-pass configuration of agar-gelatin gel embedding.
(3) " mould " dissolves: above-mentioned " mould " single-pass configuration being immersed concentration is soak 2.5 hours in the sodium citrate solution of 110mmol/L.
Observation duct is transparent, shows that column " mould " is dissolved completely, and single-pass configuration is formed.As shown in figure ip.Inject Yin's red at injection port place, observe single pass circulation further, experiment shows, single channel circulation is good.
Embodiment 7:
Gelatin-collagen mixed gel embedding calcium alginate column " mould " preparation can pour into the three-dimensional cross-linked binary channels of microfluid.
Calcium alginate column " mould " preparation in use Ca
2+solution is provided by calcium nitrate solution.
(1) preparation of microfibre column " mould ": use model is G18 syringe, by mass concentration be 2.5% sodium alginate soln with the speed of 160 μ L/s at the uniform velocity implantation concentration in 180mmol/L calcium nitrate solution, gel reaction 45 minutes, forms calcium alginate column " mould ".
(2) " mould " is embedded in gelatin-collagen gel: preparation mass concentration is the gelatin 10ml of 20mg/mL.High-pressure sterilizing pot high temperature 121 DEG C thawing.Preparation 20mg/ml mouse tail I-type collagen 10ml, regulating pH to be 7.0 under ice bath, is gelatin-collagen mixed solution with gelatin equal-volume mixed preparing.Absorption 5ml gelatin-collagen mixed gel dissolution homogeneity is flat is applied to culture dish.After cooling, two calcium alginates " mould " intersection is placed on gel, and injects entrance gel end at the whole story is reserved.Reuse 5ml gelatin-collagen solution and slowly embed " mould " structure.Room temperature cools 35 minutes, forms calcium alginate column " mould " channel structure embedded by gelatin-collagen gel.
(3) " mould " dissolves: above-mentioned " mould " channel structure being immersed concentration is soak 1.5 hours in the sodium citrate solution of 170mmol/L.
Observation duct is transparent, shows that column " mould " is dissolved completely, and binary channels is formed.As shown in Figure 2.Inject Yin's red at injection port place, observe twin-channel circulation further, experiment shows, binary channels circulation is good.
Claims (7)
1. a preparation method for micro-fluidic three dimensional gel chip model, is characterized in that the micro-fluidic chip in three dimensional biological gel internal build blood vessel access type; Comprise the steps:
The first step: the preparation of microfibre column " mould ":
Sodium alginate soln is injected ionic calcium soln, gel reaction 5 ~ 60 minutes, form calcium alginate microfibre column " mould ", by the diameter control of " mould " within the scope of 50-2000 μm;
Second step: " mould " is embedded in three dimensional biological gel:
" mould " that prepare is placed in liquid biogel mixture, after gel sets, forms the three dimensional gel being embedded with " mould ";
3rd step: use lysate to dissolve " mould ", form microchannel in three dimensional biological gel, build micro-fluidic three dimensional gel chip model.
2. the preparation method of micro-fluidic three dimensional gel chip model according to claim 1, is characterized in that: in the described first step, and the mass concentration of sodium alginate is 0.5-3%, and described calcium ion concentration is 45 ~ 200mmol/L.
3. the preparation method of micro-fluidic three dimensional gel chip model according to claim 1 and 2, is characterized in that: described ionic calcium soln is calcium chloride or calcium nitrate solution.
4. the preparation method of micro-fluidic three dimensional gel chip model according to claim 1, it is characterized in that: in the described first step, sodium alginate soln injects ionic calcium soln by syringe, and syringe needle specification is 14G-34G, and charge velocity is 20-200 μ L/s.
5. the preparation method of micro-fluidic three dimensional gel chip model according to claim 1, it is characterized in that: in described second step, biogel comprises: any one in agar, gelatin, collagen, agar-collagen, agar-gelatin, collagen-gelatin, and the mass concentration of described biogel is 5-20mg/ml.
6. the preparation method of micro-fluidic three dimensional gel chip model according to claim 1, is characterized in that: in described second step, and gel at room temperature cools 20 ~ 25 minutes and solidifies.
7. the preparation method of micro-fluidic three dimensional gel chip model according to claim 1, is characterized in that: in described 3rd step, the lysate used is sodium citrate solution, and concentration is 10 ~ 200mmol/L, and dissolution time is 0.5 ~ 5 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510826081.5A CN105381826B (en) | 2015-11-25 | 2015-11-25 | A kind of preparation method of micro-fluidic three dimensional gel chip model |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510826081.5A CN105381826B (en) | 2015-11-25 | 2015-11-25 | A kind of preparation method of micro-fluidic three dimensional gel chip model |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105381826A true CN105381826A (en) | 2016-03-09 |
| CN105381826B CN105381826B (en) | 2017-08-11 |
Family
ID=55414976
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510826081.5A Expired - Fee Related CN105381826B (en) | 2015-11-25 | 2015-11-25 | A kind of preparation method of micro-fluidic three dimensional gel chip model |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105381826B (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106238111A (en) * | 2016-07-28 | 2016-12-21 | 南京理工大学 | A kind of microcapsule preparation method based on micro-fluidic chip shear flow |
| CN106944166A (en) * | 2017-04-01 | 2017-07-14 | 太原理工大学 | A kind of method that use biometric print prepares microfluidic channel model |
| CN108071007A (en) * | 2017-11-08 | 2018-05-25 | 韩金玲 | It is a kind of to prepare nano silver wire and graphene-based calcium alginate compounded conductive fiber method |
| CN108272532A (en) * | 2018-01-24 | 2018-07-13 | 山东省科学院能源研究所 | A kind of hydrogel chip of biconial pipe cavity configuration and preparation method thereof |
| CN108890937A (en) * | 2018-06-29 | 2018-11-27 | 山东省科学院能源研究所 | A kind of preparation method of the hydrogel chip of dendroid channel design |
| CN109289947A (en) * | 2018-09-29 | 2019-02-01 | 浙江大学 | Gel base micro-fluidic chip and its manufacturing method based on secondary cross-linking |
| CN109385373A (en) * | 2017-08-11 | 2019-02-26 | 复旦大学 | For detecting the microfluid co-culture device of tumour medicine sensibility and metastasis tendency |
| CN109731143A (en) * | 2019-01-10 | 2019-05-10 | 上海大学 | A kind of method that biological structure body is Prevascularized |
| CN113186609A (en) * | 2021-04-23 | 2021-07-30 | 上海大学 | Three-dimensional biological printing method and system based on microfluid spinning |
| CN113244973A (en) * | 2021-07-15 | 2021-08-13 | 成都博奥晶芯生物科技有限公司 | Gel matrix sample application liquid, blank sample application liquid, three-dimensional gel chip and preparation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002100542A1 (en) * | 2001-06-08 | 2002-12-19 | Centre National De La Recherche Scientifique | Method of manufacturing a microfluidic structure, in particular a biochip, and structure obtained by said method_________________ |
| CN102128777A (en) * | 2010-11-24 | 2011-07-20 | 西安交通大学 | 3D (Three Dimensional) micro-fluidic structure for cell detection and preparation method thereof |
| CN102502483A (en) * | 2011-10-19 | 2012-06-20 | 浙江大学 | Method for processing polymer micro-nano composite structure based on two separated moulds |
| CN102974410A (en) * | 2012-11-06 | 2013-03-20 | 中国科学院大连化学物理研究所 | Method and dedicated chip for preparation of micron calcium alginate filament based on microfluidic chip |
| CN104941541A (en) * | 2015-05-28 | 2015-09-30 | 安徽理工大学 | System and method for preparing microcapsules |
-
2015
- 2015-11-25 CN CN201510826081.5A patent/CN105381826B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002100542A1 (en) * | 2001-06-08 | 2002-12-19 | Centre National De La Recherche Scientifique | Method of manufacturing a microfluidic structure, in particular a biochip, and structure obtained by said method_________________ |
| CN102128777A (en) * | 2010-11-24 | 2011-07-20 | 西安交通大学 | 3D (Three Dimensional) micro-fluidic structure for cell detection and preparation method thereof |
| CN102502483A (en) * | 2011-10-19 | 2012-06-20 | 浙江大学 | Method for processing polymer micro-nano composite structure based on two separated moulds |
| CN102974410A (en) * | 2012-11-06 | 2013-03-20 | 中国科学院大连化学物理研究所 | Method and dedicated chip for preparation of micron calcium alginate filament based on microfluidic chip |
| CN104941541A (en) * | 2015-05-28 | 2015-09-30 | 安徽理工大学 | System and method for preparing microcapsules |
Non-Patent Citations (2)
| Title |
|---|
| ANWARUL HASAN(ET AL.): "Microfluidic techniques for development of 3D vascularized tissue", 《BIOMATERIALS》 * |
| MARIO CABODI(ET AL.): "A Microfluidic Biomaterial", 《J.AM.CHEM.SOC.》 * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106238111A (en) * | 2016-07-28 | 2016-12-21 | 南京理工大学 | A kind of microcapsule preparation method based on micro-fluidic chip shear flow |
| CN106944166A (en) * | 2017-04-01 | 2017-07-14 | 太原理工大学 | A kind of method that use biometric print prepares microfluidic channel model |
| CN109385373A (en) * | 2017-08-11 | 2019-02-26 | 复旦大学 | For detecting the microfluid co-culture device of tumour medicine sensibility and metastasis tendency |
| CN109385373B (en) * | 2017-08-11 | 2022-01-18 | 复旦大学 | Microfluid co-culture device for detecting tumor drug sensitivity and metastasis tendency |
| CN108071007A (en) * | 2017-11-08 | 2018-05-25 | 韩金玲 | It is a kind of to prepare nano silver wire and graphene-based calcium alginate compounded conductive fiber method |
| CN108272532A (en) * | 2018-01-24 | 2018-07-13 | 山东省科学院能源研究所 | A kind of hydrogel chip of biconial pipe cavity configuration and preparation method thereof |
| CN108890937B (en) * | 2018-06-29 | 2020-07-07 | 山东省科学院能源研究所 | Preparation method of hydrogel chip with dendritic channel structure |
| CN108890937A (en) * | 2018-06-29 | 2018-11-27 | 山东省科学院能源研究所 | A kind of preparation method of the hydrogel chip of dendroid channel design |
| CN109289947A (en) * | 2018-09-29 | 2019-02-01 | 浙江大学 | Gel base micro-fluidic chip and its manufacturing method based on secondary cross-linking |
| CN109731143A (en) * | 2019-01-10 | 2019-05-10 | 上海大学 | A kind of method that biological structure body is Prevascularized |
| CN113186609A (en) * | 2021-04-23 | 2021-07-30 | 上海大学 | Three-dimensional biological printing method and system based on microfluid spinning |
| CN113244973A (en) * | 2021-07-15 | 2021-08-13 | 成都博奥晶芯生物科技有限公司 | Gel matrix sample application liquid, blank sample application liquid, three-dimensional gel chip and preparation method |
| CN113244973B (en) * | 2021-07-15 | 2021-10-08 | 成都博奥晶芯生物科技有限公司 | Gel matrix sample application liquid, blank sample application liquid, three-dimensional gel chip and preparation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105381826B (en) | 2017-08-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105381826A (en) | Preparation method of microfluidic three-dimensional gel chip model | |
| CN110004111B (en) | Preparation method of organoid sphere | |
| CN110042077B (en) | High-flux culture method of organoid spheres | |
| JP6710000B2 (en) | Micro fiber | |
| Morimoto et al. | Point-, line-, and plane-shaped cellular constructs for 3D tissue assembly | |
| CN104383604B (en) | A kind of one-stop preparation method of vascularization life structure | |
| Nie et al. | Bottom-up biofabrication using microfluidic techniques | |
| CN103160942B (en) | A kind of anisotropic fiber and preparation method thereof | |
| CN102172498B (en) | Three-dimensional porous chitosan/gelatine microsphere, preparation method thereof and application in liver cell culture | |
| CN111065422A (en) | Method of making a multilayered tubular tissue construct | |
| Lee et al. | Integration of microfluidic chip with biomimetic hydrogel for 3D controlling and monitoring of cell alignment and migration | |
| CN104840272A (en) | Printing method for three-dimensional biological structure with built-in nutrition channel | |
| Mohajeri et al. | Cell encapsulation in alginate-based microgels using droplet microfluidics; a review on gelation methods and applications | |
| CN106944166A (en) | A kind of method that use biometric print prepares microfluidic channel model | |
| Nazari et al. | Microfluidic-based droplets for advanced regenerative medicine: Current challenges and future trends | |
| US20110104777A1 (en) | Method of making an artificial micro-gland that is anisotropic | |
| JP6659059B2 (en) | Method for producing three-dimensional tissue having vasculature, and three-dimensional tissue containing gel having vasculature | |
| JP7413644B2 (en) | Microfluidic devices for culturing cells including biowalls, bead beds, and biointerfaces, and methods for modeling biointerfaces of microfluidic devices | |
| KR101726063B1 (en) | Method for verifying and improving diagnosis efficiency of fluorescence-labeled nucleic acid-liposome nano-particle for diagnosis of tumor using three-dimensional simulating human tissue system | |
| Magnani et al. | Recent Advances in Microfluidically Spun Microfibers for Tissue Engineering and Drug Delivery Applications | |
| Orabi et al. | Emerging Advances in Microfluidic Hydrogel Droplets for Tissue Engineering and STEM Cell Mechanobiology | |
| US20220088272A1 (en) | Graft and use thereof | |
| CN105802251A (en) | Self-assembly collagen template tissue engineering material as well as preparation method and application thereof | |
| Radonjić et al. | Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations | |
| Liu et al. | Advances in Microfluidic Technologies in Organoid Research |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170811 Termination date: 20211125 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |