CN103348011A - 来自植物的α-甘露糖苷酶以及使用所述α-甘露糖苷酶的方法 - Google Patents

来自植物的α-甘露糖苷酶以及使用所述α-甘露糖苷酶的方法 Download PDF

Info

Publication number
CN103348011A
CN103348011A CN2011800656049A CN201180065604A CN103348011A CN 103348011 A CN103348011 A CN 103348011A CN 2011800656049 A CN2011800656049 A CN 2011800656049A CN 201180065604 A CN201180065604 A CN 201180065604A CN 103348011 A CN103348011 A CN 103348011A
Authority
CN
China
Prior art keywords
seq
sequence
nucleotide sequence
plant
mannosidase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2011800656049A
Other languages
English (en)
Chinese (zh)
Inventor
N·V·伊万诺夫
P·坎帕诺尼
D·弗洛拉克
K·大石
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Philip Morris Products SA
Original Assignee
Philip Morris Products SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philip Morris Products SA filed Critical Philip Morris Products SA
Publication of CN103348011A publication Critical patent/CN103348011A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C12N9/2491—Beta-mannosidase (3.2.1.25), i.e. mannanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Plant Substances (AREA)
CN2011800656049A 2010-12-23 2011-12-23 来自植物的α-甘露糖苷酶以及使用所述α-甘露糖苷酶的方法 Pending CN103348011A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10196758 2010-12-23
EP10196758.6 2010-12-23
PCT/EP2011/073954 WO2012085270A1 (en) 2010-12-23 2011-12-23 Alpha-mannosidases from plants and methods for using the same

Publications (1)

Publication Number Publication Date
CN103348011A true CN103348011A (zh) 2013-10-09

Family

ID=43972036

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2011800656049A Pending CN103348011A (zh) 2010-12-23 2011-12-23 来自植物的α-甘露糖苷酶以及使用所述α-甘露糖苷酶的方法

Country Status (6)

Country Link
US (1) US20140298508A1 (https=)
EP (1) EP2655628A1 (https=)
JP (2) JP2014506128A (https=)
CN (1) CN103348011A (https=)
CA (1) CA2821660A1 (https=)
WO (1) WO2012085270A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109072248A (zh) * 2016-02-09 2018-12-21 希博斯美国有限公司 采用寡核苷酸介导的基因修复提高靶向基因修饰的效率的方法和组合物
CN115312121A (zh) * 2022-09-29 2022-11-08 北京齐碳科技有限公司 靶基因位点检测方法、装置、介质及程序产品

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012085270A1 (en) * 2010-12-23 2012-06-28 Philip Morris Products S.A. Alpha-mannosidases from plants and methods for using the same
CN107723310B (zh) * 2017-10-19 2024-01-09 北京睿诚海汇健康科技有限公司 植物作为宿主在表达卡那抗体中的应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7214786B2 (en) * 2000-12-14 2007-05-08 Kovalic David K Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
WO2009037279A1 (en) * 2007-09-18 2009-03-26 Basf Plant Science Gmbh Plants with increased yield
EP2113514A1 (de) * 2008-05-03 2009-11-04 Westfälische Wilhelms-Universität Münster Verfahren zur Erzeugung von hypoallergenen Glykoproteinen in mutierten oder transgenen Pflanzen oder Pflanzenzellen sowie mutierte oder transgene Pflanzen und Pflanzenzellen zur Erzeugung hypoallergener Glykoproteine

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19754622A1 (de) * 1997-12-09 1999-06-10 Antje Von Dr Schaewen Pflanzliche GntI-Sequenzen und Verwendung derselben zur Gewinnung von Pflanzen mit verminderter oder fehlender N-Acetylglucosaminyltransferase I (GnTI)-Aktivität
BRPI0206546B1 (pt) * 2001-01-19 2015-07-21 Phyton Holdings Llc Método para a produção secretória de glicoproteína que tem cadeia de açúcar do tipo humana, usando célula vegetal
WO2012085270A1 (en) * 2010-12-23 2012-06-28 Philip Morris Products S.A. Alpha-mannosidases from plants and methods for using the same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7214786B2 (en) * 2000-12-14 2007-05-08 Kovalic David K Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement
WO2009037279A1 (en) * 2007-09-18 2009-03-26 Basf Plant Science Gmbh Plants with increased yield
EP2113514A1 (de) * 2008-05-03 2009-11-04 Westfälische Wilhelms-Universität Münster Verfahren zur Erzeugung von hypoallergenen Glykoproteinen in mutierten oder transgenen Pflanzen oder Pflanzenzellen sowie mutierte oder transgene Pflanzen und Pflanzenzellen zur Erzeugung hypoallergener Glykoproteine

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
NO NAME: "NP_175570.1", 《NCBI》 *
NO NAME: "NP_566675.1", 《NCBI》 *
NO NAME: "ref|XM_002263448.2", 《NCBI》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109072248A (zh) * 2016-02-09 2018-12-21 希博斯美国有限公司 采用寡核苷酸介导的基因修复提高靶向基因修饰的效率的方法和组合物
CN115312121A (zh) * 2022-09-29 2022-11-08 北京齐碳科技有限公司 靶基因位点检测方法、装置、介质及程序产品

Also Published As

Publication number Publication date
JP2014506128A (ja) 2014-03-13
JP2017131225A (ja) 2017-08-03
WO2012085270A1 (en) 2012-06-28
EP2655628A1 (en) 2013-10-30
US20140298508A1 (en) 2014-10-02
CA2821660A1 (en) 2012-06-28

Similar Documents

Publication Publication Date Title
EP2611824B1 (en) Heavy metal reduction in planta
JP2017158592A (ja) 植物の酵素活性の改変
KR102442802B1 (ko) 식물 내 니코틴의 노르니코틴으로의 변환 감소
KR102012993B1 (ko) 니코티아나 타바쿰으로부터의 이소프로필말레이트 합성효소 및 그의 방법 및 용도
JP6225108B2 (ja) ニコチアナ・タバカムからのトレオニン合成酵素ならびにその方法および使用
US20100287657A1 (en) Novel Nucleotide Sequences Encoding Nicotiana Beta-1,2-Xylosyltransferase
JP2017131225A (ja) 植物由来アルファ−マンノシダーゼ及びそれを使用する方法
EP2205728A1 (en) Method to produce modified plants with altered n-glycosylation pattern
JP2025533986A (ja) Ｎｔｐ２を調節することによって葉バイオマスおよび窒素利用効率を増加させる
HK1181396B (en) Heavy metal reduction in planta
HK1181396A (en) Heavy metal reduction in planta

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20131009