CN103340413B - Preparation method for bone calcium powder from outer wall of bone through lactobacillus fermentation - Google Patents
Preparation method for bone calcium powder from outer wall of bone through lactobacillus fermentation Download PDFInfo
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- CN103340413B CN103340413B CN201310232639.8A CN201310232639A CN103340413B CN 103340413 B CN103340413 B CN 103340413B CN 201310232639 A CN201310232639 A CN 201310232639A CN 103340413 B CN103340413 B CN 103340413B
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Abstract
The invention provides a preparation method for bone calcium powder from the outer wall of a bone through lactobacillus fermentation, which belongs to the technical field of food engineering. The method comprises the following steps: cleaning a fresh bone or bone residue left after extraction of bone collagen liquid and taking the cleaned fresh bone or bone residue out for draining; delivering the fresh bone or bone residue to a pulverizer for coarse cracking; delivering the cracked fresh bone or bone residue to a bone wall separator for bone wall peeling and sieving out the outer wall of the bone; rinsing with hot water a plurality of times, draining off moisture, then carrying out mixing with water and preparing thick slurry; adding degreased whey powder into the slurry of the outer wall of the bone, allowing the degreased whey powder to be dissolved with stirring and carrying out heating and sterilization; carrying out cooling after sterilization, adding lipase and papain for enzymatic hydrolysis and carrying out cooling; adding a mixed lactobacillus strain for fermentation; ending fermentation when the concentration of a lactobacillus colony is 5 * 10<9> cfu/ml; and carrying out drying and crushing. The outer wall of the bone has highest content of mineral matters and collagen in a skeleton, and high content mineral matters can be obtained after bone wall separation. In particular, the procedures of enzymatic hydrolysis and fermentation enable macromolecular protein to be degraded and metabolites of lactobacillus to be increased, so the rate of absorption and utilization is improved.
Description
Technical field
The invention belongs to Food Engineering Development field, relate to a kind of preparation method of bone outer wall lactobacillus-fermented bone calcium powder.
Background technology
The replenishers of calcium are a lot, and calcium carbonate, minerals calcium, BIOLOGICAL CALCIUM etc. are each has something to recommend him.From animal fresh bone, separation is calcareous, if ox bone is often considered to the possibility of rabid ox disease remnants.Actually this is not so, one, and rabid ox disease is the disease at brain position, irrelevant with bone; Its two, this technique has been taked the long-time sterilization technology of HTHP, any germ do not have survival possibility.
Along with social development progress, people's average life span extends, and the whole world is own through presenting aging population.What the aged was threatened to one of maximum common disease, frequently-occurring disease is that human body DD is greatest wide in osteoporosis morbidity scope, and harm is large.Instrument fracture accidentally, causes great burden will to family and society.The elderly of a lot of paralysis only, because fallen a glue, does not just again stand up, and to eventually, not only needs health care expenditures together with sick bed, wheelchair, also needs people to take care of.In the more and more nervous future society of labour, beyond doubt very large burden.So the osteoporotic food of research opposing becomes the focus of Food Engineering Development research.From another perspective, pay close attention to the elderly's bone health, liberate exactly young man, liberation labour.
Summary of the invention
The object of this invention is to provide a kind of preparation method with the calcareous bone outer wall lactobacillus-fermented bone calcium powder that supplementary bone day by day runs off.
Bone outer wall lactobacillus-fermented bone calcium powder of the present invention is prepared in accordance with the following steps:
(1) put remaining bone slag after fresh bone or extraction bone collagen liquid into rinse bath and clean, after pulling out, put into vibrating water-dropping machine draining;
(2) then send into pulverizer coarse crushing to 0.8-1.5cm;
(3) by sending into through the bone slag of coarse crushing, in bone wall seperator, carry out bone wall and peel off, after peeling off, be sent in three layers of screening machine and sieve, from screening machine upper strata, sift out bone outer wall;
(4) by bone outer wall with 60-100 ° of C hot water rinsing for several times, after dissolving and remove, drain away the water to surface fat, the ratio that is then 1:5-8 with water according to weight ratio is mixed, and beating underflow to fineness is 160-200 order;
(5) in bone outer wall slurries Shen, add the fat-free comminuted whey of its weight 4-10%, stirring and dissolving while being warming up to 90-98 ° of C, keeps sterilization in 30-40 minute;
(6) while being cooled to 2 ° of C of 45 scholars after sterilization, drop into account for substrate gross weight 0.1-0.3% lipase, account for the papain of substrate gross weight 0.1-0.3%, after enzymolysis 2-4 hour, cooling;
(7) while being cooled to 2 ° of C of 37 scholars, the mixed lactic bacterial classification that input accounts for substrate gross weight 0.2-0.5% ferments, and fermentation temperature is 2 ° of C of 37 scholar;
(8) Xiao's lactobacillus concentration reaches 5XlO
9when cfu/ml is above, fermentation ends;
(9) put into hot air circulation low-temperature bake oven and be dried, then pulverize, obtain bone outer wall lactobacillus-fermented bone calcium powder.
Tool of the present invention has the following advantages:
1, bone outer wall is bone junket mineral and the high position of collagen content, and through bone wall, separation can obtain the mineral matter that relative amount is higher.Especially enzymatic hydrolysis and fermentation technique, increases lactic acid bacteria metabolite when can degrade high molecular weight protein, thereby improves absorption rate.
2, material complete utilization, production process does not produce tailing after-treatment problem.
3, complete raw-food material, additive-free, edible safety, and long-term edible without accumulating murder by poisoning threat, mouthfeel is taken like a shot.
Accompanying drawing explanation
Fig. 1 is the structural representation of bone wall seperator;
Fig. 2 is the front view of bone wall seperator;
Fig. 3 is the structural representation of peeling off assembly;
Fig. 4 is that the G of Fig. 3 is to view;
Fig. 5 is the cutaway view of taking away the rotating shaft after blade.
The specific embodiment
Below in conjunction with embodiment, technical scheme of the present invention is further explained to explanation; but do not limit protection scope of the present invention; every technical solution of the present invention is modified or is equal to replacement; and do not depart from the spirit and scope of technical solution of the present invention, all should be encompassed in guarantor of the present invention and expand scope Shen.
The specific embodiment one: present embodiment is made each bone outer wall lactobacillus-fermented bone calcium powder in accordance with the following steps:
(1) the remaining bone slag that slaughter pig, ox, sheep, donkey, horse, chicken, duck, goose or kangaroo cut meat after the fresh bone of livestock and poultry animal of rejecting or extraction bone collagen liquid is put rinse bath into and is cleaned, and puts into vibrating water-dropping machine draining after pulling out;
(2) then send into pulverizer coarse crushing to 0.8-1.5cm;
(3) by sending into through the bone slag of coarse crushing, in bone wall seperator, carry out bone wall and peel off, after peeling off, be sent in three layers of screening machine and sieve, from screening machine upper strata, sift out bone outer wall;
(4) by bone outer wall with 60-100 ° of C hot water rinsing for several times, after dissolving and remove, drain away the water to surface fat, the ratio that is then 1:5-8 with water according to weight ratio is mixed, and beating underflow to fineness is 160-200 order;
(5) spend the fat-free comminuted whey that adds its weight 4-10% in bone outer wall slurries, stirring and dissolving while being warming up to 90-98 ° of C, keeps sterilization in 30-40 minute;
(6) while being cooled to 2 ° of C of 45 scholars after sterilization, drop into account for substrate gross weight 0.1-0.3% lipase, account for the papain of substrate gross weight 0.1-0.3%, stirring 15 per minute turned under condition after enzymolysis 2-4 hour, cooling; Lipase and papain are produced by Nanning Pang Bo bioengineering Co., Ltd, and enzyme activity is 70XlO
4u/g.
(7) while being cooled to 37+2 ° of C, the mixed bacteria that input accounts for substrate gross weight 0.2-0.5% ferments, and fermentation temperature is 37+2 ° of C; Described mixed bacteria is mixed by lactobacillus acidophilus and streptococcus thermophilus, its Shen, and lactobacillus acidophilus accounts for the 30-70% of bacterial classification weight, and streptococcus thermophilus accounts for the 30-70% of bacterial classification weight; Stirring 15 per minute turns; Its bacterium lives and is 10
9-10
10cfu/m, bacterial classification derives from Chinese agriculture microorganism fungus kind preservation administrative center, and deposit number is ACCCl0637 ACCCl1073.
(8) when lactobacillus concentration reaches 5XlO
9when cfu/ml is above, fermentation ends;
(9) put into hot air circulation low-temperature bake oven and be dried, be then crushed to 60-100 order, obtain bone outer wall lactobacillus-fermented bone calcium powder.Dry run: start moisture discharge device after charging, open the valve that send clean hot blast, temperature variation curve in drying box: room temperature is put in storage to 45-55 ° of C and maintained 16-20 hour, during stir one to secondary; When dry materials is during to water content 5-20%, close hot blast air intake valve, exhausting moisture valve is opening still, is cooled to room temperature and delivers from godown.
The specific embodiment two: present embodiment is different from the specific embodiment one, take and extract bone outer wall separated after bone egg oral fluid and prepare bone outer wall lactobacillus-fermented bone calcium powder as raw material, and concrete steps are as follows:
One, pre-treating technology: take negative pressure of vacuum extraction process to remove watery blood in fresh bone: extractor is watery blood in malleation, surge tank remove fresh bone under the condition of negative-pressure vacuum conversion.That is: between extractor and vavuum pump, be connected a surge tank, under extractor malleation, surge tank negative-pressure vacuum condition, when 20-60 ° of C of extractor instrument displays temperature, repeatedly rise pressure release all of a sudden, before watery blood is not solidified below C at 60 °, with pressure, release, concrete steps are as follows:
(1) get fresh bone 10OOkg and soak after 20 hours, rinsing stripping and slicing 5-lOcm ,-30 ° of C, after freezing 8 hours, pack in extractor and sealing;
(2) start vavuum pump, open and connect surge tank valve, make surge tank negative pressure, during vacuum 14-75mmhg, close vavuum pump;
(3) open extractor inner sleeve steam valve, be warming up to and represent that temperature is that 20-50 ° of C, tank internal pressure are closed intake valve while being 0.01-0.lMPA;
(4) open gradually surge tank and connect extractor valve, by melt the watery blood suction surge tank that from bone Shen, when pressure returns 0, open surge tank bottom valve, emptying surge tank, then closes valve, surge tank bottom valve between surge tank and extractor;
(5) repeat number time step (2) (4) is released watery blood before not solidifying with pressure;
(6) opening the spray valve that extracts tank body top rinses the blood that bone surface adheres to for 1-5 minute and melts the blood foam that to stay.Utilize negative-pressure vacuum degree 14-75mmhg in surge tank, pump it to surge tank, when pressure returns 0, open surge tank bottom valve, emptying surge tank; Then close valve, surge tank bottom valve between surge tank and extractor;
(7) start extractor inner sleeve steam valve and vavuum pump, in extractor temperature 40-60 ℃, close intake valve during tank internal pressure 0.01-0.lMPA; During surge tank vacuum 14-75mmhg, close vavuum pump;
(8) slowly open the relief valve between extractor and surge tank, in the pipeline viewing mirror in the middle of extractor and surge tank, observe, when the material color of extracting out is sat yellow-white gradually from brown, the extracting of indication watery blood finishes.When surge tank negative pressure returns 0, open valve put in clean surge tank take watery blood as main material standby to fertilizer workshop.In extractor, material is waited to enter next step and is removed fat and extraction bone collagen operation.
Two, collagen mixed liquor extraction process: remove after watery blood, then use the same method that my carry bone collagen mixed liquor, concrete steps are as follows:
(9) open extractor inner sleeve and jacket steam valve and be warming up to that inner sleeve represents temperature 60-10O ° C, the closed clip inner sleeve air valve that spouts while representing that pressure rises to 0.1-0.13Mpa; Start vavuum pump, open and connect surge tank valve, make surge tank negative pressure, during vacuum 14-75mmhg, close;
(10) slowly open the valve between surge tank and extractor, will take fat as main mixed liquor suction surge tank, until surge tank to negative pressure returns 0, emptying material, then closes valve, surge tank bottom valve between surge tank and extractor;
(11) repeating step (9)-(10); in pipeline viewing mirror between extractor and surge tank Shen, observe; when the material its colour changed into yellow white of extracting out and when milky white gradually; the fat of fresh bone Shen removes step to be finished; in emptying surge tank, take fat as main material, then close valve, surge tank bottom valve between surge tank and extractor;
(12) open extractor inner sleeve and jacket steam valve, closed clip inner sleeve intake valve when extractor inner sleeve represents temperature 90-12l ° C, tank internal pressure 0.1-0l5MPa; Start vavuum pump, make surge tank negative pressure, during vacuum 14-75mmhg, close; Slowly open the valve between surge tank and extractor, by bone collagen liquid suction surge tank, until surge tank to negative pressure returns 0, emptying surge tank, then closes valve, surge tank bottom valve between surge tank and extractor;
(13) repeating step (12) is 3-6 time, in the pipeline viewing mirror in the middle of extractor and surge tank, can observe, and when clumsy material is milky white, obtains bone collagen liquid.When extract concentration declines gradually, material color is by dense milky white while thinning out gradually, and indication is extracted and closed to an end.After bone collagen liquid suction surge tank, surge tank negative pressure is returned 0 o'clock, opens bottom discharge valve door, and the bone collagen liquid in emptying surge tank is to temporary storage tank.
(14) open extractor mouth, the great trolley that connects material that enters of bone by after extracting, is transported to next procedure standby.
Three, bone wall separating technology:
(15) bone slag enters rinse bath, puts into vibrating water-dropping machine draining after pulling out.Clean waste water and enter the processing of collection Shen, fertilizer workshop.
(16) conveyer belt connecting by draining machine is sent bone slag into pulverizer and is carried out coarse crushing to 0.8-1.5cm, is resent to the feed hopper of bone wall seperator, in stripping hedge storehouse, shells hedge.Bone wall is peeled off and has been utilized cancellous bone and compact bone substance to cut bearing of extruding force different to grinding, with the 6-12 sheet blade on axle center with peel off the distance between storehouse, the amount that adjustable material passes through and while peeling off stone roller cut the power of extruding.Rotating shaft band cutting blade is along counterclockwise Universal rotary; Feed hopper is from the charging of rotating shaft top, and bone floods to enter to be peeled off storehouse and be limited in later and peel off between bulkhead and blade, and bone slag, along with the rotation of rotating shaft, is subject to the extruding of scraping stone roller and rotating shaft and peeling off bulkhead of blade, forms uneven grain or irregular sheet.Bone wine after peeling off is discharged from discharging opening, is sent in three layers of screening machine.
During screening according to the separated bone outer wall of granular size and bone inwall.Cancellous bone becomes more tiny particle through grinding to cut to push, and from screening machine lowermost layer, spills bone inwall, is brown, and after pulling an oar together with watery blood, organic wastewater, enzymatic hydrolysis and fermentation is produced fertilizer; Compact bone substance is harder, and after stone roller is cut extruding, particle is still greater than cancellous bone, and from screening machine upper strata, aperture 5-8mm sifts out bone outer wall, and low temperature drying after making beating enzymatic hydrolysis and fermentation, as calcium-supplementing preparation raw material; What wherein aperture, screening machine middle level 4-5mm sifted out is the mixture that is mixed with bone inside and outside wall, and low temperature drying after making beating enzymatic hydrolysis and fermentation, as feed for pet additive.Through peeling off and sieving, can make the best use of everything, improve added value.
As Figure 1-5; described bone wall seperator comprises successively feed hopper 1 from top to bottom, peels off storehouse 2 and discharging opening 3; feed hopper 1, peel off storehouse 2, discharging opening 3 communicates; peel off rotating stripping assembly is installed in storehouse 2; described rotating stripping assembly is comprised of 6-12 sheet blade 4 and rotating shaft 5, and the axle center of rotating shaft 5 is positioned on the cross central line of peeling off storehouse 3; Blade 4 and its cutting edge 8 courts that the axial plane of rotating shaft 5 is along the circumferential direction evenly provided with several inclinations expose outside, and it is adjustable that cutting edge 8 exceeds 2-lOmm compared with rotating shaft 5 faces,
In said apparatus, the length of described blade 4 is consistent with the length of rotating shaft 5, and thickness is 3-8mm.
In said apparatus, the end of described rotating shaft 5 be arranged on the bearing 7 of peeling off 3 two ends, storehouse and be connected, can control by bearing 7 rotating speed of rotating shafts 5.
In said apparatus, described in peel off the cylindrical shape that is shaped as in storehouse 2.
Said apparatus Shen, the axial plane of described rotating shaft 5 along the circumferential direction mills out 6-12 the tapered plane groove 6 of settling blade 4, and tapered plane grain 6 is evenly distributed on the axial plane of rotating shaft 5; its inclination angle 30-45 degree; bevel depth is as the criterion with blade thickness 3-8mm, and length is consistent with rotating shaft, and width is consistent with blade width; On the inclined-plane of tapered plane groove 6, be provided with circular hole 11, on blade 4, be provided with oval slotted eye 9, in oval poor hole 9 and circular hole, be inserted with screw 10, blade 4 is fixed in tapered plane grain 6 by screw 10, and the cutting edge of blade 4 is that 2-lOmm is adjustable with the distance of peeling off storehouse 2 inwalls.On every blade 4, be provided with 3 oval notches 9 for fixed screw 10 corresponding with rotating shaft 5, for adjustable blade cutting edge 8 and peel off the distance between bulkhead.
Operation principle is as follows:
Bone wall is peeled off and has been utilized cancellous bone and compact bone substance to cut bearing of extruding force different to grinding, with turning the 6-12 sheet blade on uranium and peeling off the distance between storehouse, the amount that adjustable material passes through and while peeling off stone roller cut the power of extruding.Rotating shaft band cutting blade is along counterclockwise rotation; Feed hopper is from turning the charging of uranium top, and bone gulf enters peels off cabin and be limited in later and peel off between bulkhead and blade, and bone slag, with the rotation of right spindle, is subject to the extruding of scraping stone roller and rotating shaft and peeling off bulkhead of blade, forms uneven grain.
Four, prepare bone outer wall lactobacillus-fermented bone calcium powder technique:
(17) by bone outer wall 60-100.C hot water floats Shen for several times, drains away the water, and the ratio that is then 1:5-8 with water according to weight ratio is mixed, and beating underflow to fineness is 160-200 order;
(18) in bone outer wall slurries Shen, add the fat-free comminuted whey of its weight 4-10%, stirring and dissolving while being warming up to 90-98 ℃, keeps sterilization in 30-40 minute;
(19) while being cooled to 2 ° of C of 45 scholars after sterilization, drop into account for substrate gross weight 0.1-0.3% lipase, account for the papain of substrate gross weight 0.1-0.3%, stirring 15 per minute turned under condition after enzymolysis 2-4 hour, cooling;
(20) while being cooled to 2 ℃ of 37 scholars, dropping into the mixed bacteria that accounts for substrate gross weight 0.2-0.5% and ferment, fermentation temperature is 2 ° of C of 37 scholar; Described mixed bacteria is mixed by lactobacillus acidophilus and streptococcus thermophilus, and wherein, lactobacillus acidophilus accounts for the 30-70% of bacterial classification weight, and streptococcus thermophilus accounts for the 30-70% of bacterial classification weight; Stirring 15 per minute turns.
(21) when lactobacillus concentration reaches 5XlO
9when cfu/ml is above, fermentation ends;
(22) put into hot air circulation low-temperature bake oven and be dried, then pulverize, obtain bone outer wall lactic acid bacteria baking powder.Dry run: start moisture discharge device after charging, open the valve that send clean hot blast, temperature variation curve in drying box: room temperature is put in storage to 45-55 ℃ and maintained 16-20 hour, during stir one to secondary; When dry materials is during to water content 5-20%, close hot blast air intake valve, exhausting moisture valve is opening still, is cooled to room temperature and delivers from godown.
Claims (4)
1. a preparation method for bone outer wall lactobacillus-fermented bone calcium powder, is characterized in that described method carries out in accordance with the following steps:
(1) put remaining bone slag after fresh bone or extraction bone collagen liquid into rinse bath and clean, after pulling out, put into vibrating water-dropping machine draining;
(2) then send into pulverizer coarse crushing to 0.8-1.5cm;
(3) by sending into through the bone slag of coarse crushing, in bone wall seperator, carry out bone wall and peel off, after peeling off, be sent in three layers of screening machine and sieve, from screening machine upper strata, sift out bone outer wall, aperture, upper strata 5-8mm, described bone wall seperator comprises feed hopper (1) from top to bottom successively, peel off storehouse (2) and discharging opening (3), feed hopper (1), peel off storehouse (2), discharging opening (3) communicates, peel off in storehouse (2) rotating stripping assembly is installed, described rotating stripping assembly is comprised of 6-12 sheet blade (4) and rotating shaft (5), the axle center of rotating shaft (5) is positioned on the cross central line of peeling off storehouse (2), blade (4) and its cutting edge (8) court that the axial plane of rotating shaft (5) is along the circumferential direction evenly provided with several inclinations expose outside, it is adjustable that cutting edge (8) exceeds 2-10mm compared with rotating shaft (5) face, the length of blade (4) is consistent with the length of rotating shaft (5), thickness is 3-8mm,
(4) by bone outer wall with 60-100 ℃ of hot water rinsing for several times, after dissolving and remove, drain away the water to surface fat, the ratio that is then 1:5-8 with water according to weight ratio is mixed, and beating underflow to fineness is 160-200 order;
(5) in bone outer wall slurries, add the fat-free comminuted whey of its weight 4-10%, stirring and dissolving while being warming up to 90-98 ℃, keeps sterilization in 30-40 minute;
(6) while being cooled to 45 ± 2 ℃ after sterilization, drop into account for substrate gross weight 0.1-0.3% lipase, account for the papain of substrate gross weight 0.1-0.3%, after enzymolysis 2-4 hour, cooling;
(7) while being cooled to 37 ± 2 ℃, the mixed lactic bacterial classification that input accounts for substrate gross weight 0.2-0.5% ferments, and fermentation temperature is 37 ± 2 ℃;
(8) when lactobacillus concentration reaches 5 * 10
9when cfu/ml is above, fermentation ends;
(9) put into hot air circulation low-temperature bake oven and be dried, then pulverize, obtain bone outer wall lactobacillus-fermented bone calcium powder.
2. the preparation method of bone outer wall lactobacillus-fermented bone calcium powder according to claim 1, it is characterized in that in described step (9) dry run, after charging, start moisture discharge device, the valve of clean hot blast is sent in unlatching, temperature variation curve in drying box: room temperature is put in storage to 45-55 ℃ and maintained 16-20 hour, during stir one to secondary; When dry materials is during to water content 5-20%, close hot blast air intake valve, exhausting moisture valve is opening still, is cooled to room temperature and delivers from godown.
3. the preparation method of bone outer wall lactobacillus-fermented bone calcium powder according to claim 1, is characterized in that described fresh bone is the fresh bone of livestock and poultry animal that slaughter pig, ox, sheep, donkey, horse, chicken, duck, goose or kangaroo cut meat and reject.
4. the preparation method of bone outer wall lactobacillus-fermented bone calcium powder according to claim 1, it is characterized in that described mixed lactic bacterial classification is mixed by lactobacillus acidophilus and streptococcus thermophilus, wherein, lactobacillus acidophilus accounts for the 30-70% of bacterial classification weight, and streptococcus thermophilus accounts for the 30-70% of bacterial classification weight.
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| CN106509654A (en) * | 2016-11-18 | 2017-03-22 | 四川理工学院 | Preparation method of dried meat rich in organic calcium and activity probiotics |
| CN107319364A (en) * | 2017-07-26 | 2017-11-07 | 贵州大学 | A kind of bone acid of unique local characteristic |
| CN108157976B (en) * | 2017-11-30 | 2021-05-04 | 广州城市职业学院 | Method for preparing digestible sturgeon active calcium powder through two-step fermentation and prepared calcium powder |
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