Summary of the invention
The object of the present invention is to provide a kind of extraction process of new enzymolysis bone calcium, described technique can better be removed fat in the sclerotin than prior art.
Another object of the present invention provides a kind of more healthy calcium supplementing product production technology, and the calcium supplementing product that described technique makes has lower fat content, has again splendid mouthfeel simultaneously.
Another object of the present invention provides a kind of calcium supplementing product with market competitiveness, and described product is not because of degreasing and go the design of smelling of fish or mutton technique to increase production cost,
For achieving the above object, the present invention adopts following technical proposal to realize.
The present invention adopts three degreasing modes to remove animal oil, is 60~80 ℃ for the first time, 45~75min; 80~90 ℃ for the second time, 30min; The lipase enzymolysis for the third time.Three degreasings also have the effect of sterilization simultaneously except having the fatty effect of removing in the sclerotin.For strengthening degreasing effect, the present invention preferably is broken to bone meal 5~40mm particle, the too small production cost that then increases of particle, and the excessive degrease effect of particle is relatively poor.The inventor determines finally that through test of many times and experience 5~40mm granulometric range reaches the best cost performance of bone fat and cost.
After the alcohol steep degreasing, the present invention also adopts the hot-water soak rinse method to remove the fat that exists in the aggregate; Again with 4000~8000r/min, centrifugal 30min can remove most fat in the aggregate like this after the rinsing.
For further removing remaining fat, process choice of the present invention again adds alkaline lipase and carries out fat remaining in the enzymolysis aggregate after centrifugal.Alkaline lipase A, B account for 0.1~0.4%, 20~30 ℃ of aggregate weight ratios by 1.5~3: 1 compound lipases that mixes, hydrolysis 40~60min.Behind enzymolysis, secondary centrifuging is further removed remaining trace fat.
The present invention adopts the method for fermentation that the aggregate before fermenting is carried out preliminary treatment, and then carries out enzymolysis and extraction calcium element, and then extract is carried out fermentation process, removes the peculiar smell in the extract, improves mouthfeel.
For strengthening ferment effect, the present invention preferably is broken to aggregate 5~40mm particle, the too small production cost that then increases of particle, and the excessive effect of particle is relatively poor.The inventor determines finally that through test of many times and experience 5~40mm granulometric range reaches the best cost performance of the extraction of calcium element and cost.
Enzymolysis primary fermentation of the present invention adopts lactic acid bacteria and aggregate by certain weight proportion, and 35~50 ℃, fermentation 12h.The weight proportion of lactic acid bacteria and aggregate, lactic acid bacteria concentration, fermentation temperature and the time in zymotic fluid has important impact to the product Free Calcium content that finally makes, and the weight proportion of lactic acid bacteria and aggregate and the lactic acid bacteria concentration in zymotic fluid having the greatest impact to free calcium.The inventor determines after the repetition test that according to oneself working experience for many years it is 0.01~0.04% that lactic acid bacteria accounts for the aggregate weight ratio, under the condition of aggregate and water w/v 1: 3~5, can reach the best cost performance of input and output.
The inventor provides great many of experiments, it is unexpected that the various lactobacillus of finding ferment according to certain proportioning combination, final product Free Calcium content improves more than 8% with certain lactobacillus-fermented more separately, and it is better to adopt bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and three kinds of lactic acid bacterias of Lactobacillus helveticus (L.helviticus) to carry out ferment effect; Adopt these three kinds of lactic acid bacteria proportioning after fermentation effects best, when three kinds of lactic acid bacterias make up with weight ratio at 1: 3: 2, improve 10%~30% behind the more single lactobacillus-fermented of enzymolysis liquid Free Calcium content.
Secondary fermentation of the present invention adopts leuconostoc cremoris and citric acid, and the proportioning between the two all has important impact to taste, the production efficiency of end product.The inventor determines that finally the end product effect of fermentation is better under the condition of leuconostoc cremoris and citric acid w/v 1: 20~30.Under these conditions, determine that fermentation temperature is 39 ℃, when fermentation time is 12h, can reach the optimization of production efficiency.
The present invention adopts the method for high-temperature sterilization to reach the purpose of sterilizing, also removes the lactic acid bacteria in the enzymolysis liquid simultaneously.
Enzymolysis of the present invention adopts the complex enzyme hydrolysis method, and after screening, final discovery is carried out enzymolysis with bromelain and papain and had better effect than other protease.Certainly, select other protease to carry out enzymolysis and also can realize purpose of the present invention.Best enzymolysis scheme is the compound protease that zymotic fluid adds bromelain and papain weight ratio 1: 2~5 in the extraction process of the present invention, and adding citric acid is transferred pH value to 4~6,40~60 ℃ stirring 40~60min.
The used compound protease of hydrolysis aggregate accounts for aggregate weight ratio 0.05~0.2%.
The alkaline lipase that alkaline lipase A is produced by the Penicillium expansion PF868 of bioengineering institute of Fujian Normal University development, Co., Ltd provides by the green little health bioengineering in Shenzhen; Alkaline lipase B adopts biotechnology to be made with extra care and the alkaline lipase of generation by fungi fermentation, is provided by Haining Jin Chao Industrial Co., Ltd..
The various formulations that auxiliary material such as starch, dextrin, lactose, microcrystalline cellulose, HPMC, polyethylene glycol, dolomol, superfine silica gel powder, xylitol, lactitol, glucose, glycine, sweet mellow wine, glycine etc. are mixed on composition of the present invention and any or more than one pharmacies, for example, can be made into tablet, sustained release tablets, dripping pill, granule, capsule, fine granule.Preferred dosage form is tablet or granule.
Adopt in the bone meal that technique of the present invention makes, fat is completely removed substantially, so that fat content is lower than 0.1% in the calcium agent that enzymolysis obtains.Product safety and health more concerning the consumer that angiocardiopathy is arranged, but financial burden therefore increased again.
Test example
Below further specify beneficial effect of the present invention by some concrete experimental datas, all embodiment of the present invention all can make close therewith experiment effect, following data just illustrate.
1, materials and methods
1.1 ox bone: be purchased from the Hebei good fortune and become five rich food limited companies
1.2 sample 1: the sample that uses the ox bone acquisition according to Chinese patent CN1087793A; Sample 2: the sample that uses the ox bone acquisition according to US Patent No. 6342252B1; Sample 3: according to " variation of ultramicro grinding yak bone paste free calcium and amino-acid nitrogen behind fermentation and enzymolysis " (Chen Dan, Zhang Chuanlin etc., total the 178th phase of " China brewages " the 1st phase in 2008) sample of " 1.5 lactobacillus-fermenteds are processed yak bone paste ... ox bone mud distilled water diluting is 10% concentration ... ferment 36 hours " use ox bone acquisition in; Sample 4: the sample that uses the ox bone acquisition according to the embodiment of the invention 4.
1.3 method: adopt atomic absorption spectroscopy determination free calcium content.
2, result
2.1 the free calcium content of sample 1 is 184.21mg/100g, the free calcium content of sample 2 is 313.09mg/100g, and the free calcium content of sample 3 is 2013.56mg/100g, and the free calcium content of sample 4 is 2501.16mg/100g.
2.2 through the Data Comparison analysis, the free calcium content of the prepared product of the present invention is compared with sample 1, sample 2, has the raising of highly significant; Compare with sample 3, have significant raising, increase rate reaches 24.22%.
The specific embodiment
Preparation culture medium: MRS culture medium: peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 2%, dipotassium hydrogen phosphate 0.2%, sodium acetate 0.5%, magnesium sulfate 0.02%, manganese sulfate 0.005%, Tween-80 0.1%, Triammonium citrate 0.2%, PH5.5~6.0,115 ℃, sterilization 30min.
Embodiment 1
Get animal skeleton and be crushed to the 40mm particle, add ethanol, 80 ℃, lixiviate 75min abandons supernatant; 90 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 10, add alkaline lipase A, B by the compound lipases of mixing in 1.5: 1, account for 0.4%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, gets bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratios 1: 3: 2; Aggregate and water w/v 1: 5 add 5% sucrose of gross weight, and it is 0.04%, 50 ℃ that lactic acid bacteria accounts for the aggregate weight ratio, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 2 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.05%, and adding citric acid is transferred pH value to 5.5, and 40 ℃ are stirred 1h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.06% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 30, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.04%.
Embodiment 2
Get animal skeleton and be crushed to the 5mm particle, add ethanol, 60 ℃, lixiviate 45min abandons supernatant; 80 ℃ of hot-water soak rinsing 30min, with aggregate 4000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 3 are transferred pH value 8, add alkaline lipase A, B by the compound lipases of mixing in 3: 1, account for 0.1%, 20 ℃ of aggregate weight ratio, and hydrolysis 40min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, gets bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratios 1: 3: 2; Aggregate and water w/v 1: 3 add 5% sucrose of gross weight, and it is 0.01%, 35 ℃ that lactic acid bacteria accounts for the aggregate weight ratio, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 5 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.2%, and adding citric acid is transferred pH value to 6.5, and 60 ℃ are stirred 2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.02% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 20, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.05%.
Embodiment 3
Get animal skeleton and be crushed to the 20mm particle, add ethanol, 70 ℃, lixiviate 60min abandons supernatant; 85 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 4 are transferred pH value 9, add alkaline lipase A, B by the compound lipases of mixing in 2: 1, account for 0.3%, 25 ℃ of aggregate weight ratio, and hydrolysis 50min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, gets bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratios 1: 3: 2; Aggregate and water w/v 1: 4 add 5% sucrose of gross weight, and it is 0.03%, 40 ℃ that lactic acid bacteria accounts for the aggregate weight ratio, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 3 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.08%, and adding citric acid is transferred pH value to 6, and 50 ℃ are stirred 1.5h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.04% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 25, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.04%.
Embodiment 4
Get animal skeleton and be crushed to the 10mm particle, add ethanol, 80 ℃, lixiviate 75min abandons supernatant; 90 ℃ of hot-water soak rinsing 30min, with aggregate 6000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 3.5 are transferred pH value 8.5, add alkaline lipase A, B by the compound lipases of mixing in 2: 1, account for 0.2%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, gets bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacterias; Aggregate and water w/v 1: 5 add 5% sucrose of gross weight, and it is 0.04%, 50 ℃ that lactic acid bacteria accounts for the aggregate weight ratio, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 4 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.1%, and adding citric acid is transferred pH value to 6.4, and 55 ℃ are stirred 1h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.03% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 30, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.07%.
Embodiment 5
Get animal skeleton and be crushed to the 25mm particle, add ethanol, 75 ℃, lixiviate 65min abandons supernatant; 86 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 4 are transferred pH value 9.5, add alkaline lipase A, B by the compound lipases of mixing in 2.8: 1, account for 0.3%, 30 ℃ of aggregate weight ratio, and hydrolysis 50min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, gets bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratios 1: 3: 2; Add 5% sucrose of gross weight, it is 0.04%, 40 ℃ that lactic acid bacteria accounts for the aggregate weight ratio, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 3.5 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.09%, and adding citric acid is transferred pH value to 5.5, and 45 ℃ are stirred 2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.05% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 25, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.05%.
Embodiment 6
Get animal skeleton and be crushed to the 35mm particle, add ethanol, 78 ℃, lixiviate 70min abandons supernatant; 87 ℃ of hot-water soak rinsing 30min, with aggregate 7000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 8, add alkaline lipase A, B by the compound lipases of mixing in 1.8: 1, account for 0.25%, 20 ℃ of aggregate weight ratio, and hydrolysis 50min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, gets bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacterias; Aggregate and water w/v 1: 5 add 5% sucrose of gross weight, and it is 0.04%, 45 ℃ that lactic acid bacteria accounts for the aggregate weight ratio, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 4.5 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.15%, and adding citric acid is transferred pH value to 6.1, and 48 ℃ are stirred 2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.05% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 22, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.04%.
Embodiment 7
Get animal skeleton and be crushed to the 10mm particle, add ethanol, 60 ℃, lixiviate 75min abandons supernatant; 88 ℃ of hot-water soak rinsing 30min, with aggregate 5000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 4 are transferred pH value 10, add alkaline lipase A, B by the compound lipases of mixing in 3: 1, account for 0.35%, 24 ℃ of aggregate weight ratio, and hydrolysis 45min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, get bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacterias add 5% sucrose of gross weights, it is 0.03% that lactic acid bacteria accounts for the aggregate weight ratio, 50 ℃, fermentation 12h, 120 ℃ of sterilization 30min; With the compound protease of aggregate zymotic fluid adding bromelain and papain weight ratio 1: 2~5, compound protease accounts for aggregate weight ratio 0.06%, and adding citric acid is transferred pH value to 5.8, and 58 ℃ are stirred 1.2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.04% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 30, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.05%.
Embodiment 8
Get animal skeleton and be crushed to the 15mm particle, add ethanol, 80 ℃, lixiviate 75min abandons supernatant; 89 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 8.5, add alkaline lipase A, B by the compound lipases of mixing in 2.6: 1, account for 0.14%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, get bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratios 1: 3: 2,5% the sucrose that adds gross weight, 35~50 ℃, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 2.5 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.12%, and adding citric acid is transferred pH value to 6.5, and 50 ℃ are stirred 2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.05% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 30, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.04%.
Embodiment 9
Get animal skeleton and be crushed to the 30mm particle, add ethanol, 75 ℃, lixiviate 55min abandons supernatant; 85 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 8, add alkaline lipase A, B by the compound lipases of mixing in 3: 1, account for 0.35%, 28 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, and the extracting lactic acid bacterium joins in the aggregate, and it is 0.04% that lactic acid bacteria accounts for the aggregate weight ratio, and aggregate and water w/v 1: 3 add 5% sucrose of gross weight, and 50 ℃, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 4.8 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.18%, and adding citric acid is transferred pH value to 6.5, and 60 ℃ are stirred 2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.03% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid w/v 1: 28, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.03%.
Embodiment 10
Get animal skeleton and be crushed to the 20mm particle, add ethanol, 80 ℃, lixiviate 60min abandons supernatant; 84 ℃ of hot-water soak rinsing 30min, with aggregate 6000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 10, add alkaline lipase A, B by the compound lipases of mixing in 3: 1, account for 0.3%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, get bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratios add in the aggregate at 1: 3: 2,5% the sucrose that adds gross weight, 40 ℃, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 3 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.05%, and adding citric acid is transferred pH value to 5.5, and 40 ℃ are stirred 2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.05% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid w/v 1: 26, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.04%.
Embodiment 11
Bone is crushed to the 40mm particle, adds ethanol, 80 ℃, lixiviate 75min abandons supernatant; 83 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 10, add the compound lipases that alkaline lipase A, B mix, and account for 0.1%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, and the extracting lactic acid bacterium adds in the aggregate, and aggregate and water w/v 1: 5 add 5% sucrose of gross weight, and 42 ℃, fermentation 12h, 120 ℃ of 30min that sterilize; With the compound protease of aggregate zymotic fluid adding bromelain and papain weight ratio 1: 2~5, compound protease accounts for aggregate weight ratio 0.13%, and adding citric acid is transferred pH value to 6, and 60 ℃ are stirred 2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.02% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid w/v 1: 20, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.05%.
Embodiment 12
Bone is crushed to the 5mm particle, adds ethanol, 60 ℃, lixiviate 45min abandons supernatant; 82 ℃ of hot-water soak rinsing 30min, with aggregate 4000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 3 are transferred pH value 8, add compound lipases, account for 0.1%, 20 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, gets bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratios 1: 3: 2; Aggregate and water w/v 1: 4 add 5% sucrose of gross weight, and it is 0.04%, 45 ℃ that lactic acid bacteria accounts for the aggregate weight ratio, fermentation 12h, 120 ℃ of sterilization 30min; With the compound protease of aggregate zymotic fluid adding bromelain and papain weight ratio 1: 2~5, compound protease accounts for aggregate weight ratio 0.2%, and adding citric acid is transferred pH value to 6.5, and 46 ℃ are stirred 1h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.05% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid w/v 1: 30, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.04%.
Embodiment 13
Bone is crushed to the 30mm particle, adds ethanol, 75 ℃, lixiviate 60min abandons supernatant; 81 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 4 are transferred pH value 9, add compound lipases, account for 0.4%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, gets bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratios 1: 3: 2; Aggregate and water w/v 1: 3.5 add 5% sucrose of gross weight, and it is 0.04%, 50 ℃ that lactic acid bacteria accounts for the aggregate weight ratio, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 4 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.07%, and adding citric acid is transferred pH value to 6.5, and 40 ℃ are stirred 2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.05% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid w/v 1: 30, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.06%.
Embodiment 14
Bone is crushed to the 10mm particle, adds ethanol, 80 ℃, lixiviate 75min abandons supernatant; 84 ℃ of hot-water soak rinsing 30min, with aggregate 8000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 5 are transferred pH value 10, add the compound lipases that alkaline lipase A, B mix, and account for 0.4%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, gets bifidobacterium lactis (B.lactis), lactobacillus bulgaricus (L.bulgaricus) and Lactobacillus helveticus (L.helviticus), three kinds of lactic acid bacteria weight ratios 1: 3: 2; Aggregate and water w/v 1: 5 add 5% sucrose of gross weight, and it is 0.04%, 50 ℃ that lactic acid bacteria accounts for the aggregate weight ratio, fermentation 12h, 120 ℃ of sterilization 30min; The aggregate zymotic fluid is added bromelain and 1: 4 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.07%, and adding citric acid is transferred pH value to 6.5, and 40 ℃ are stirred 2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.06% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid w/v 1: 26, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.04%.
Embodiment 15
Bone is crushed to the 25mm particle, adds ethanol, 80 ℃, lixiviate 65min abandons supernatant; 84 ℃ of hot-water soak rinsing 30min, with aggregate 6000r/min, centrifugal 30min abandons supernatant; Aggregate after centrifugal is added water, and aggregate and water w/v 1: 4 are transferred pH value 8.5, add compound lipases, account for 0.3%, 30 ℃ of aggregate weight ratio, and hydrolysis 60min is centrifugal, removes supernatant; Centrifugal rear aggregate adds water, and the extracting lactic acid bacterium adds in the aggregate, and it is 0.02% that lactic acid bacteria accounts for the aggregate weight ratio, and aggregate and water w/v 1: 4 add 5% sucrose of gross weight, and 50 ℃, fermentation 12h, 120 ℃ of 30min that sterilize; The aggregate zymotic fluid is added bromelain and 1: 4 compound protease of papain weight ratio, and compound protease accounts for aggregate weight ratio 0.07%, and adding citric acid is transferred pH value to 6.5, and 40 ℃ are stirred 2h; Separating liquid, residue repeat to extract again; Said extracted liquid is mixed, add leuconostoc cremoris and citric acid, it is 0.03% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid w/v 1: 30, fermentation temperature are 39 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of deactivations are drying to obtain.Adopt " Determination of in the GB/T5009.6 food " regulation detection method that bone meal is carried out the determination of fat, recording the product fat content is 0.04%.