CN103323628B - Micro-electrode clamper of electric physiological experiment - Google Patents
Micro-electrode clamper of electric physiological experiment Download PDFInfo
- Publication number
- CN103323628B CN103323628B CN201310291314.7A CN201310291314A CN103323628B CN 103323628 B CN103323628 B CN 103323628B CN 201310291314 A CN201310291314 A CN 201310291314A CN 103323628 B CN103323628 B CN 103323628B
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- microelectrode
- base plate
- hard substrate
- fixed
- flexible gasket
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- 238000002474 experimental method Methods 0.000 title claims abstract description 10
- 239000000758 substrate Substances 0.000 claims abstract description 21
- 239000000463 material Substances 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 239000007767 bonding agent Substances 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 230000007831 electrophysiology Effects 0.000 abstract description 5
- 238000002001 electrophysiology Methods 0.000 abstract description 5
- 238000000034 method Methods 0.000 description 11
- 239000002390 adhesive tape Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000004936 stimulating effect Effects 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012402 patch clamp technique Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000004018 waxing Methods 0.000 description 1
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Abstract
The pipette holder that a kind of electro physiology experiment uses, comprise: some microelectrodes, the cantilever be arranged symmetrically with and base plate, wherein: cantilever comprises shape all-in-one-piece flexible gasket and hard substrate two parts, flexible gasket is used for fixing microelectrode, a point of hard substrate to be fixed on base plate and to rotate around this point of fixity, and the spacing making to be located at respectively the microelectrode on two cantilevers being arranged symmetrically with is adjustable.The present invention makes experimenter conveniently accurately can regulate microelectrode position, and can conveniently fix, and easily realizes keeping the desired pitch between microelectrode tip.Structure of the present invention is simple, handled easily, and can facilitate adjustment and Long-Time Service repeatedly, can reduce original time making the cost of microelectrode fixed base plate, improve electro physiology conventional efficient.
Description
Technical field
What the present invention relates to is a kind of device of instrument technical field, specifically a kind of Micro-electrode clamper of electric physiological experiment.
Background technology
At present, the stimulating electrode used in the experiment of field potential record, Patch-clamp techniques and relevant electro physiology is master mainly with bipolar electrode, requires the distance between microelectrode tip to be tending towards infinite approach but does not contact (tip is less than 0.1mm apart).For electro physiology experiment, microelectrode distance between two tips is crossed conference and is had a strong impact on effect of stimulation and experimental result, is unacceptable.In prior art, the fixing of bipolar electrode still generally adopts the method making fixing egative film by hand, its concrete grammar is: cut out a block-shaped suitable filter paper, filter paper first pre-fixes two microelectrodes with adhesive tape, manually adjust the position of microelectrode under the microscope, make the tip of two microelectrodes close as far as possible, finally on the filter paper fixing microelectrode position, burn coating of wax, make microelectrode finally fixing and sealed insulation.The weak point of the method is: 1) use and manually push away, dial the position that the manual method such as microelectrode is difficult to fine adjustment microelectrode.2) pre-fix microelectrode by the method for filter paper and adhesive tape and can not stablize fixing microelectrode, slightly interference or shake just can destroy the position that microelectrode originally kept, and are difficult to make to keep desirable distance between microelectrode tip.3) in the process of waxing, due to the cooling of wax, and high temperature wax juice is on the impact of filter paper and adhesive tape, all very likely makes the microelectrode originally fixed depart from original position, makes can not keep desirable distance between microelectrode tip.4) said method often needs repeated multiple timesly just can obtain desirable microelectrode pitch, and repeatedly repeats the time that said process usually needs to expend a few hours, seriously reduces conventional efficient, brings very large inconvenience to experimental implementation.
Summary of the invention
The present invention is directed to prior art above shortcomings, a kind of Micro-electrode clamper of electric physiological experiment is provided, conveniently accurately can regulate and stablize the position fixing microelectrode, reducing experimenter and spend in original time made on microelectrode fixed base plate, improve conventional efficient; Be applicable to the experiment of field potential, patch-clamp and relevant electrophysiology.
The present invention is achieved by the following technical solutions, the present invention includes: some microelectrodes, the cantilever be arranged symmetrically with and base plate, wherein: cantilever comprises shape all-in-one-piece flexible gasket and hard substrate two parts, flexible gasket is used for fixing microelectrode, a point of hard substrate to be fixed on base plate and to rotate around this point of fixity, and the spacing making to be located at respectively the microelectrode on two cantilevers being arranged symmetrically with is adjustable.
Described flexible gasket is positioned at top, and hard substrate is positioned at below, and both are fixed by cementing agent.
The outside of described hard substrate has deep-slotted chip breaker, and after hard substrate rotates, fixture is arranged in deep-slotted chip breaker and is fixed by hard substrate.
Fixed by bonding agent between described microelectrode and flexible gasket.
Described base plate has pickup groove, and this pickup groove is connected with external micro-behaviour's fixed handle.
Described hard substrate and the material of base plate are organic glass.
The material of described flexible gasket is silica gel.
Technique effect
Compared with prior art, beneficial effect of the present invention comprises: 1) structure is simple, easy to operate, with low cost.Microelectrode fixation principle is very easy, through test, can complete the ideal distance accurately kept between microelectrode tip in several minutes.Compared to time a few hours spent needed for background technology, greatly can shorten Production Time, improve conventional efficient.2) by the mode of hinged cantilever in base plate plane, can very easily by most advanced and sophisticated for microelectrode close to minimum distance.And the position of repeated multiple times conciliation microelectrode can be facilitated.3) set bolt being used for fixing cantilever is placed on cantilever to extend on outer egative film, and set bolt in the deep-slotted chip breaker of cantilever egative film, has larger contact area and good fixed effect with egative film by inlay card.Meanwhile, because set bolt is direct securing elongated spiral arm egative film outside, therefore when the bolt that is tightened is with fixing microelectrode position, this process of hand Screw bolt has an impact to the position of original microelectrode hardly.This beneficial effect improves the disturbance and impact that manual operation in fixation procedure produces microelectrode position greatly, reduces the time making and expend needed for desirable stimulating electrode, improves the precision of making and the efficiency of experiment.4) prolonged and repeatedly can use, when more renewing electrode, only need again with cementing agent, microelectrode be fixed on cantilever, while improving conventional efficient, also can reduce experimental cost.
Accompanying drawing explanation
Fig. 1 is stereographic map of the present invention;
Fig. 2 is vertical view of the present invention;
Fig. 3 is side view of the present invention;
Fig. 4 is the vertical view of spiral arm;
Fig. 5 is the side view of spiral arm;
Fig. 6 is the vertical view of base plate.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment
As shown in Figure 1, Figure 2 and Figure 3, the present embodiment comprises: some microelectrodes 1, two cantilevers 2 be arranged symmetrically with and base plate 3, wherein: cantilever 2 comprises flexible gasket 2-1 and hard substrate 2-2 two parts, flexible gasket 2-1 is used for fixing microelectrode 1, and the point of hard substrate 2-2 to be fixed on base plate 3 and to rotate around this point of fixity; The spacing making to be located at respectively the microelectrode 1 on two cantilevers 2 being arranged symmetrically with is adjustable.
As shown in Fig. 4, Fig. 6, the hard substrate 2-2 having deep-slotted chip breaker is fixed on base plate 3 by set bolt 4, its deep-slotted chip breaker is by being fixed on the screwing bolts 5 as fixture on base plate 3, and spiral arm 2 can its set bolt 4 be axle, turns at base plate 3 plane internal rotation.And can be screwed rear fixing by screwing bolts 5, whole spiral arm 2 is fixed.
Flexible gasket 2-1 is positioned at top, and hard substrate 2-2 is positioned at below, and both are fixed by cementing agent.
As shown in Figure 5, fixed by bonding agent 8 between described microelectrode 1 and flexible gasket 2-1.
Described base plate 3 has pickup groove, and external micro-behaviour's fixed handle 7 is fixed by outer connecting bolt 6 and base plate 3.
Described hard substrate 2-2 and the material of base plate 3 are organic glass.
The material of described flexible gasket 2-1 is silica gel.
Claims (1)
1. a Micro-electrode clamper of electric physiological experiment, it is characterized in that, comprise: some microelectrodes, the cantilever be arranged symmetrically with and base plate, wherein: cantilever comprises shape all-in-one-piece flexible gasket and hard substrate two parts, flexible gasket is used for fixing microelectrode, a point of hard substrate to be fixed on base plate and to rotate around a point of fixity, and the spacing making to be located at respectively the microelectrode on two cantilevers being arranged symmetrically with is adjustable;
Described flexible gasket is positioned at top, and hard substrate is positioned at below, and both are fixed by cementing agent;
The outside of described hard substrate has deep-slotted chip breaker, and after hard substrate rotates, fixture is arranged in deep-slotted chip breaker and is fixed by hard substrate;
Fixed by bonding agent between described microelectrode and flexible gasket;
Described base plate has pickup groove, and this pickup groove is connected with external micro-behaviour's fixed handle;
Described hard substrate and the material of base plate are organic glass;
The material of described flexible gasket is silica gel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310291314.7A CN103323628B (en) | 2013-07-11 | 2013-07-11 | Micro-electrode clamper of electric physiological experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310291314.7A CN103323628B (en) | 2013-07-11 | 2013-07-11 | Micro-electrode clamper of electric physiological experiment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103323628A CN103323628A (en) | 2013-09-25 |
| CN103323628B true CN103323628B (en) | 2015-10-28 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310291314.7A Expired - Fee Related CN103323628B (en) | 2013-07-11 | 2013-07-11 | Micro-electrode clamper of electric physiological experiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103323628B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2426335Y (en) * | 2000-06-22 | 2001-04-11 | 尹卫靖 | Electrophysiologic test electrode fixing holder |
| CN2824837Y (en) * | 2005-08-12 | 2006-10-11 | 成都仪器厂 | Experimental apparatus for electrophysiological examination |
| CN2868190Y (en) * | 2005-12-22 | 2007-02-14 | 覃建民 | Experimental invivo thrombus formation determining instrument |
| CN101534898A (en) * | 2006-07-31 | 2009-09-16 | 贾斯-杰斯有限责任公司 | Bipolar stimulation/recording device with widely spaced electrodes |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2912893B1 (en) * | 2007-02-23 | 2009-12-11 | Philippe Brunswick | ELECTROPHYSIOLOGICAL ANALYSIS SYSTEM |
-
2013
- 2013-07-11 CN CN201310291314.7A patent/CN103323628B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2426335Y (en) * | 2000-06-22 | 2001-04-11 | 尹卫靖 | Electrophysiologic test electrode fixing holder |
| CN2824837Y (en) * | 2005-08-12 | 2006-10-11 | 成都仪器厂 | Experimental apparatus for electrophysiological examination |
| CN2868190Y (en) * | 2005-12-22 | 2007-02-14 | 覃建民 | Experimental invivo thrombus formation determining instrument |
| CN101534898A (en) * | 2006-07-31 | 2009-09-16 | 贾斯-杰斯有限责任公司 | Bipolar stimulation/recording device with widely spaced electrodes |
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| Publication number | Publication date |
|---|---|
| CN103323628A (en) | 2013-09-25 |
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Granted publication date: 20151028 |
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