CN103308671B - A kind of detection film and detection system - Google Patents
A kind of detection film and detection system Download PDFInfo
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- CN103308671B CN103308671B CN201310192357.XA CN201310192357A CN103308671B CN 103308671 B CN103308671 B CN 103308671B CN 201310192357 A CN201310192357 A CN 201310192357A CN 103308671 B CN103308671 B CN 103308671B
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Abstract
The invention provides a kind of new detection film and detect the detection system of film for this, one end of this detection film is provided with magnetizable material, and this detection system comprises for carrying the base detecting groove, and described base is provided with field generator for magnetic.Detection film of the present invention may be used for the various biochemistry detection can carried out on film and adopts the reaction of Western blot detection material, therefore maintain common detection film and can detect the advantage of chemical substance by fast and low-cost, detection film of the present invention can be fixed on by magnetic field and detect in groove simultaneously, can avoid in testing process, detect film to be moved, to overturn or to incline, solve again the misoperation occurred when using common detection film to detect, avoid the systematic error detected.
Description
Technical field
The present invention relates to the film detected for fields such as biochemical and immunity, the novel detection film of Aulomatizeted Detect in enormous quantities can be used for specifically, the invention still further relates to the detection system for this detection film.
Background technology
Biochemistry detection is by biochemical method in broad terms, to the method that various physiologically substance detects, specifically, comprises biochemical test, immunological test, Micro biological Tests, molecular biology inspection etc.Along with the development of membrane technology, a lot of material to be checked can realize fast and low-cost and detect on film.Described detection film comprises nitrocellulose membrane, nylon membrane, PVDF membrane etc.
The binding ability of above-mentioned 3 kinds of films to nucleic acid and albumen is very high, is often used in the detection of multiple biochemical indicator.PVDF membrane (polyvinylidenefluoride) is a kind of solid support conventional in immunoblotting.Pvdf membrane is hydrophobic, and membrane aperture varies, and along with the continuous reduction of membrane aperture, film is more firm to low-molecular-weight protein combination.The albumen being greater than 20000 selects the film of 0.45um, and the albumen being less than 20000 selects the film of 0.2um.Pvdf membrane has higher physical strength, is the ideal solid phase support material in blotting.Detection plasma proteins can be widely used in, lipoprotein, glycoprotein, in the reaction of fetus alpha globulin based on this film of above characteristic.
Nitrocellulose membrane, it is the most widely used transfer medium of Western blot, has very strong binding ability to albumen, and is applicable to various coloration method, comprises isotope, chemiluminescence (Luminol class), conventional colour developing, dyeing and fluorescence developing; Background is low, and signal to noise ratio (S/N ratio) is high.Nylon membrane is a kind of long-chain polyamide film of synthesis, has very strong binding ability to nucleic acid and protein, and cellulose nitrate film can be replaced to be used for molecular engram and hybrid experiment.
Above-described by tunica fibrosa carry out substantially all comprising in biochemistry detection or immunoassay procedures such as application of sample, rinsing, colour developing etc. operation, if need the sample size of detection few, this operating process can manually complete, but if has the manually operated work of sample needing detection in enormous quantities to be very heavy.Inventor finds that tunica fibrosa is more crisp, easily rolls up again, abandons or topples in the process of waste liquid taking out, and test paper displacement can occur or incline or block drawing liquid suction nozzle, is very careful, is therefore difficult to be applied to Aulomatizeted Detect in enormous quantities in operating process.This just proposes new requirement to detection fibers film.
The existing detection system for film detection generally comprises the sample chamber of Systems for optical inspection and carrying sample, sample chamber comprises a carrying check-out console or detects the platform (base) of groove, part base can shake thus reach the function such as rinsing, colour developing, but as mentioned above, detect that film is easily subjected to displacement in the solution, overturns, the problem such as curling, in the process of hatching, detect film displacement can occur or incline or block drawing liquid suction nozzle, current detection system is also difficult to address these problems, and thus there will be systematic error.
Summary of the invention
An object of the present invention is to provide a kind of detection film detected on groove that can be fixed on, in order to solve problems such as detecting film is easily subjected to displacement in the solution, overturns, curling and blocking drawing liquid suction nozzle.
Another object of the present invention is to provide a kind of detection system for above-mentioned detection film.
Principle of the present invention arranges magnetisable material in one end of existing detection film, is operationally fixed in detection groove by detection film by magnetic field.
Particularly, for achieving the above object, the present invention adopts following technical scheme:
A kind of detection film, comprises detection film body, has magnetisable material in the one end detecting film body.This magnetisable material can be coated in film, is coated on film or is attached on film.
The above-mentioned detection film that can do magnetization treatment at end is all detection films being applicable to biochemistry detection or immune detection on the market, includes but not limited to nitrocellulose filter (NC film), cellulose acetate membrane (CA film), nylon membrane (PA film), polysulfone membrane (PS film), polyvinylidene fluoride film (pvdf membrane), polyester film (PET film), polypropylene screen (PP film), glass fibre membrane etc.
Above-mentioned magnetisable material can be magnetizable metal or nonmetal, includes but not limited to the materials such as iron, cobalt, nickel, or their magnetizable alloys.
The present invention also provides a kind of detection system for above-mentioned detection film, and this detection system comprises for carrying or the base of fixed test groove, and described base is provided with field generator for magnetic.The preferred electromagnetic field generator of described field generator for magnetic or permanent magnetic material.
For being adapted at carrying out manual operation outside detecting instrument, the present invention also provides a kind of and detects groove, and this detection groove is provided with field generator for magnetic at its body bottom portion, the preferred electromagnetic field generator of described field generator for magnetic or permanent magnetic material.
In the present invention, magnetisable material refers to and can adsorb under the influence of a magnetic field, but itself does not possess the material of magnetic.Common metal magnetisable material is iron, cobalt, nickel, and non-metallic magnetizable material is boron, carbon, silicon, phosphorus etc., also has some metal alloys as ferroaluminium, ferro-silicium etc.Above-mentioned magnetizable material is placed in magnetic field and can magnetizes to saturated, is therefore provided with magnetic, and the magnetic material of tool has homopolar-repulsion, the attribute that heteropole repels each other.
Permanent magnetic material is under the guiding of external magnetic field, make the spin of magnetisable material become with electronic angular momentum fixed-direction to arrange, and have magnetic, and after leaving magnetic field, magnetic can not disappear.Permanent magnetic material itself can produce magnetic field, attracts ferromagnetic material as metals such as iron, nickel, cobalts.Permanent magnetic material can be natural, such as loadstone, also can be made, such as rare earth permanent-magnetic material, ferrite sintered body, rubber magnet etc.
Detection film of the present invention is in testing process, when being placed in groove, magnetic field is applied at bottom land, by magnetic field, bottom land is fixed in one end of film, owing to fixing one end of film in reaction or rinse cycle, therefore can not overturn, in the process of hatching, also can not there is displacement or incline or block drawing liquid suction nozzle in test paper.Detection film of the present invention can be used for the various biochemistry detection can carried out on film, is therefore the advantage that existing normal film detects fast and low-cost detection, solves again the systematic error problem of Aulomatizeted Detect.
Accompanying drawing explanation
Fig. 1 is the structural representation detecting film;
Fig. 2 places the detection groove (side view) detecting film;
Fig. 3 is the vertical view that Fig. 2 detects groove.
In figure, 1 is detect film body (detecting portion for detecting), and 2 is detect one end that film body has magnetisable material, and 3 for detecting groove body, and 4 is field generator for magnetic, and 5 is groove.
Embodiment
Embodiment is used for further illustrating of the present invention below, but is not used for limiting invention which is intended to be protected.
Embodiment 1 biochemistry detection film
As shown in Figure 1, detection film of the present invention comprises the detecting portion of detection film body 1(for detecting), in the one end detecting film body 1, there is magnetisable material.This detects film is apply magnetizable material in one end of common detection film, and therefore this detects film with the general size of common detection film.The mode of coating is that magnetizable material is directly coated in one end of detection film, seals with pressing machine; Or be directly coated in one end of film; Can on the medium of adhesion material or magnetisable material is coated in that one side has, then the upper one side adhered to is covered with the plastic foil easily taken off, tear in use cover plastic foil, directly be affixed on film, said method is applicable to the detection film (nitrocellulose membrane, nylon membrane etc.) of the various dissimilar different model that present biochemistry detection is commonly used.As can be seen here, detect film and comprise the character that detecting portion has common detection film, there is the advantage that normal film detects for clinical detection fast and low-cost.
Magnetizable material can be the common material such as iron, cobalt, nickel.
Embodiment 2 detection system
Detection system of the present invention comprises for carrying the base detecting groove, described base is provided with field generator for magnetic, when being put on this base containing the detection groove detecting film, one end of detection film containing magnetisable material just can be fixed to the bottom detecting groove, therefore solves and detect the problems such as film generation upset or displacement in Aulomatizeted Detect process.
An example of detection system of the present invention is: comprise Systems for optical inspection, pipettor, base, controller, described base is used for carrying or fixed test groove, base is provided with electromagnetic field generator, base is also connected with and jolts device, Systems for optical inspection is used for detecting the chromatogram change of biochemistry detection film, pipettor for sampling, application of sample and remove the solution detected in groove, described controller control both optical detection system, pipettor and base carry out sequencing work.Systems for optical inspection, pipettor and base also can human interventions, carry out semi-automation work.
Embodiment 3 detects groove
As shown in Figures 2 and 3, the present invention detects groove and comprises detection groove body 3, field generator for magnetic 4 is had at detection groove body 3, field generator for magnetic 4 can with the clamping of detection groove body 1, be convenient to remove and install, also field generator for magnetic can be used for different detection grooves, increase the utilization factor of field generator for magnetic.Be placed on this detection groove by described detection film, produce magnetic field by field generator for magnetic, the one end of the detection film containing magnetisable material just can be fixed to the bottom detecting groove groove 5, can solve to detect film and overturn, be shifted or incline and the problem such as.
Claims (8)
1. detect a film, comprise detection film body, it is characterized in that, one end of described detection film body has magnetizable material, this detection film can be fixed on detect in groove by magnetic field.
2. detect film as claimed in claim 1, it is characterized in that, described magnetizable Material coating in detect film body surface, be coated on to detect in film body or be attached to and detect on film.
3. detect film as claimed in claim 1 or 2, it is characterized in that, described detection film body is nitrocellulose filter, cellulose acetate membrane, nylon membrane, polysulfone membrane, polyvinylidene fluoride film, polyester film, polypropylene screen or glass fibre membrane.
4. detect film as claimed in claim 1 or 2, it is characterized in that, described magnetizable material chosen from Fe, cobalt, nickel or its magnetizable alloy.
5. the detection system for detection film according to claim 1, comprise the base of the detection groove for carrying or detect described in fixed placement film, it is characterized in that, described base is provided with field generator for magnetic, and described detection film can be fixed on and detect in groove by the magnetic field produced by described field generator for magnetic.
6. detection system as claimed in claim 5, it is characterized in that, described field generator for magnetic is electromagnetic field generator or permanent magnetic material.
7. for a detection groove for detection film according to claim 1, comprise and detect groove body, it is characterized in that, have field generator for magnetic at detection groove body, described detection film can be fixed on and detect in groove by the magnetic field produced by described field generator for magnetic.
8. detection groove according to claim 7, is characterized in that, described field generator for magnetic is electromagnetic field generator or permanent magnetic material.
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| CN201310192357.XA CN103308671B (en) | 2013-05-22 | 2013-05-22 | A kind of detection film and detection system |
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| CN201310192357.XA CN103308671B (en) | 2013-05-22 | 2013-05-22 | A kind of detection film and detection system |
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| CN103308671B true CN103308671B (en) | 2016-02-03 |
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| CN111122631A (en) * | 2020-01-07 | 2020-05-08 | 中国科学院微生物研究所 | Magnetic suction type net carrying frame device |
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Effective date of registration: 20160111 Address after: 102206 Building No. 31, 1 science Road, Beijing, Changping District Applicant after: BEIJING CONTROLS & STANDARDS BIOTECHNOLOGY CO., LTD. Applicant after: Beijing Wantai Biological Pharmacy Enterprise Co., Ltd. Address before: 102206 No. 31 science Road, Beijing, Changping District Applicant before: Beijing Wantai Biological Pharmacy Enterprise Co., Ltd. |