CN103275865A - Cell specimen screening and separation device - Google Patents
Cell specimen screening and separation device Download PDFInfo
- Publication number
- CN103275865A CN103275865A CN2013102425285A CN201310242528A CN103275865A CN 103275865 A CN103275865 A CN 103275865A CN 2013102425285 A CN2013102425285 A CN 2013102425285A CN 201310242528 A CN201310242528 A CN 201310242528A CN 103275865 A CN103275865 A CN 103275865A
- Authority
- CN
- China
- Prior art keywords
- main body
- circumferential
- circle
- conical head
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000926 separation method Methods 0.000 title abstract description 8
- 238000012216 screening Methods 0.000 title abstract 4
- 230000007246 mechanism Effects 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 19
- 238000001914 filtration Methods 0.000 claims description 22
- 239000002245 particle Substances 0.000 abstract description 13
- 238000004458 analytical method Methods 0.000 abstract description 4
- 238000000605 extraction Methods 0.000 abstract description 4
- 238000001125 extrusion Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 31
- 239000007788 liquid Substances 0.000 description 12
- 230000003321 amplification Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000004698 Polyethylene Substances 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000005086 pumping Methods 0.000 description 2
- 241000258957 Asteroidea Species 0.000 description 1
- 206010042566 Superinfection Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 210000002503 granulosa cell Anatomy 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A cell specimen screening and separation device belongs to the technical field of cell analysis appliances, solves the problem of major-diameter cells in an extracted specimen, and is provided with a split main body (1); left and right circular/conical seal heads (2 and 3) which are formed through wholistic processing and provided with extrusion pipes are arranged at two ends of the split main body (1), and are provided with detachable left and right seal covers (6 and 7) matched with the left and the right circular/conical seal heads; filter screen embedding mechanisms (11) embedded with thick and thin filter screens (8 and 9) are respectively arranged on inner walls at two end parts of the main body (1); the cell specimen screening and separation device is non-electric, is convenient to operate, and can ensure that large and small particles in the cell specimen are separated out rapidly and stably, the successful extraction of large-aperture cells is realized, and the whole operation process is sealed, so that the health of operators is facilitated greatly; and the cell specimen screening and separation device is a disposable product, is low in cost, and is easy to manufacture, store, transport and apply on a large scale, and raw materials are easy to access.
Description
Technical field
The invention belongs to cell analysis utensil technical field, relate to the cell specimen screen selecting and separating mechanism.
Background technology
At present, the large and small granulosa cell sample of domestic separation (mixed solution) is handled annual more than 1,000 ten thousand parts, cell chaff interference in the type specimen is a lot, comprise big, little two kinds of particles, all need adopt certain physical means to be removed, therefore, whether the quality that the sample cell granulations separates directly has influence on experimental result accurate.The method of extracting the major diameter cell in the sample has two kinds, and be external introduction, first kind, with the micro-pore-film filtration pumping equipment mixing liquid is directly aspirated, small-particle and certain liquid are drained by filter membrane (pore size filter is less than 10um, and after the aperture was strengthened, film strength was not enough) under suction function, and big or middle particle is trapped together with the major diameter cell, thereby realize that the major diameter cell separates with short grained, afterwards, again to the macrobead otherwise processed; Second kind, with whizzer mixing liquid is directly separated, thereby realize separating of major diameter cell and large and small particle; The weak point of first method is; because the pore size filter of microporous membrane is little in during negative pressure-pumping; as to its increasing, film strength is not enough, easily breaks; therefore; often because of the fenestra of the too many and blocking microporous film of small-particle in the mixing liquid, easily cause and separate not exclusively or separate and fail, and macrobead also needs otherwise processed; the weak point of second method is; operate too loaded down with trivial detailsly, need get test tube in addition, be numbered mark earlier; add sample liquid again; add parting liquid again, after centrifugal, outwell upper liquid, stay test tube bottom cluster of grains; for the second time add parting liquid, after bigger centrifugal force is centrifugal, outwell top liquid.Though through two times centrifugal repeatedly, but for the proportion macrobead in this way difficult removal similar to maxicell, in addition, operator are taken place easily in violation of rules and regulations, such as the numbering error, the possibility that causes makeing mistakes increases, and can not well macrobead be separated, and therefore influence final experiment effect, thereby derive a wrong conclusion.Simultaneously, above-mentioned two kinds of separation methods, uncap (especially second method) handled, and the unknown contagium infectivity that may exist in the cell specimen is given the operator.External (containing corollary apparatus) the cost height of introducing has the people imitated, and therefore brings many troubles, even invite lawsuit.
Summary of the invention
The technical problem that solves:
A kind of cell specimen screen selecting and separating mechanism is provided, the major diameter cell that adopts the present invention to be used for the extraction cell specimen can overcome the deficiencies in the prior art, by using the filter screen in different apertures, utilize gravity principle that large and small particle separation is gone out, obtain the middle particle that contains the major diameter cell, not only be convenient to operation, alleviate workload, and can guarantee the result that separation proposes, realization is to the successful extraction of wide aperture cell, therefore do not influence final experiment effect, whole process is sealing treatment simultaneously, is conducive to operator's health greatly.
The technical scheme that adopts:
The cell specimen screen selecting and separating mechanism has filter screen, it is characterized in that: have the clamshell type cylindrical body that can be screwed onto together, the left and right two ends of main body have circle or the conical head that processes with main body integral body respectively; Has an extruding pipe that is communicated with and processes with circle or conical head integral body with the tube chamber of main body on the top of each circle or conical head respectively; Also have with each circle or conical head together with the supporting corresponding capping of the extruding pipe on it, what capping can detachable property is contained on the main body; Two ends in main body are bordering on the inwall at the position that is connected with each circle or conical head, have the filtering net setting-in mechanism of circumferential laying respectively, and thin, coarse filtration net is inlaid in respectively in the filtering net setting-in mechanism.
Beneficial effect:
The cell specimen screen selecting and separating mechanism, be exclusively used in big in the isolated cell sample, small-particle, to extract the major diameter cell, be used for scientific experiment, can overcome the deficiencies in the prior art, using method and working specification are easy to grasp, can not electricity consumption, be convenient to operation, be difficult for error, can guarantee safe big with in the cell specimen fast, separate small is gone out, and realizes the successful extraction to the wide aperture cell, therefore also just can guarantee final experimental result, separate big simultaneously, small-particle, the operation whole process of extracting the major diameter cell is sealing treatment, and the unknown contagium that can effectively avoid may existing in the cell specimen infects to the operator, is conducive to operator's health greatly; The change that the present invention has essence to prior art can fill the domestic gaps, and easy to implement, is easy to apply; Cell specimen screen selecting and separating mechanism of the present invention is disposable product, once uses the back to concentrate special disposal, can effectively avoid contingent superinfection and to the pollution of environment; Used starting material are easy to get, and be easy to processing and fabricating in enormous quantities, storage, transportation, and cost are low.
Description of drawings
Fig. 1, overall structure synoptic diagram;
Fig. 2, press the amplification I figure shown in Fig. 1;
Fig. 3, press the amplification II figure shown in Fig. 1;
Fig. 4, press the amplification III figure shown in Fig. 1;
Fig. 5, press the amplification IV figure shown in Fig. 1;
Fig. 6, analyse and observe by the A-A shown in Fig. 5;
Fig. 7, press the amplification V figure shown in Fig. 1;
Fig. 8, analyse and observe by the B-B shown in Fig. 7.
Embodiment
Further explanation in detail by reference to the accompanying drawings;
Shown in Fig. 1～8, the cell specimen screen selecting and separating mechanism has the clamshell type cylindrical body 1 that can be screwed onto together, and the left and right two ends of main body 1 have left and right circle or the conical head 2,3 that processes with main body 1 integral body respectively; Has a left and right extruding pipe 4,5 that is communicated with the tube chamber 10 of cylindrical shell 1 and processes with circle or conical head integral body on left and right circle or conical head 2,3 the top respectively; Also have with left and right circle or conical head 2,3 together with the left and right extruding pipe 4 on it, 5 supporting corresponding left and right cappings 6,7, left and right capping 6,7 is being contained on the main body 1 of detachable property respectively; Two ends in main body 1 are bordering on the inwall at the position that is connected with left and right circle or conical head 2,3, have the filtering net setting-in mechanism 11 of circumferential laying respectively, and thin, coarse filtration net 8, appraise and select one in 9 fens are inlaid in the filtering net setting-in mechanism 11; Described being spirally connected, namely main body 1 is screwed onto together by fore shaft position 18 mutually by left and right two portions of clamshell type tubular.
As shown in Figure 4, left and right capping 6,7 structure that is contained on the main body 1 of detachable property respectively is the type of being spirally connected, and has internal thread 12 on left and right capping 6,7 the port inner wall, has on the corresponding outer wall of main body 1 and internal thread 12 supporting corresponding outside screws 13.
Shown in Fig. 5,6, the structure of filter screen setting-in mechanism 11 is the circumferential recess formula, two ends in main body 1 are bordering on the inwall at the position that is connected with left and right circle or conical head 2,3, has the twice convex formula circumferential hoop 14 that processes with main body 1 integral body, has circumferential recess 15 therebetween, the internal diameter Φ of the internal diameter of circumferential recess 15 and main body 1
0Equate its width m
0Be 1.5～2mm; The height h of convex formula circumferential hoop 14 and width m
1Be 1.5～2mm; As Fig. 7, shown in 8, the structure of filter screen setting-in mechanism 11 is circumferential slit type, two ends and a left side in main body 1, right circle or conical head 2,3 are bordering on the inwall at position of connection, has the circumferential crack of being formed by one convex formula circumferential hoop 14 and circumferentially uniform interior raised line 16 17, convex formula circumferential hoop 14 is positioned at by circular head one side, circumferentially the specification of the circumferential recess 15 in the corresponding specification of crack 17 and the described circumferential recess formula structure is identical, the height of interior raised line 16 and width are identical with the corresponding size of convex formula circumferential hoop 14, and interior raised line 16 is four～eight of uniform numbers circumferentially; Thin during application, coarse filtration net 8,9 diameter must not be less than the internal diameter Φ of main body 1
0, filtering net should be able to safely be inlaid in the filtering net setting-in mechanism 11 reliably---and be in circumferential recess 15 or the circumferential crack 17.
Select for use transparent and have certain elastic plastic and make, as PE (polyethylene) or PP (polypropylene); The specification of finly filtration net 8 is 500～700 orders, and the specification of coarse filtration net 9 is 170～230 orders, is middle starfish auspicious (Beijing) Science and Technology Ltd. commercially available prod;
By shown in Figure 1, concrete specification is as follows, the mm of unit:
In the main body 1, the long L of left half
140～110, the long L of right half
210～40, internal diameter Φ
025～35;
The high Y of the radius of circular head or conical head equals the radius of main body 1;
Wall thickness δ 2～3.5;
Left and right extruding pipe 4,5 long L
315～30, internal diameter Φ
11～2;
Be described as follows for the example using method with Fig. 1:
Finly filtration net 8 is inlaid in the filtering net setting-in mechanism 11 in the left half of main body 1, and coarse filtration net 9 is inlaid in the filtering net setting-in mechanism 11 in the right half of main body 1;
1, turns on left and right two portions of main body 1 from fore shaft position 18, and cell specimen is poured in the left half, by fore shaft position 18 left and right two portions are screwed onto together more afterwards;
2, left end left standstill 10 minutes or placed whizzer low speed (500～2000 rev/mins) to rotate down 2 minutes, afterwards, open following left sealing cap 6, manual pinching main body 1, process finly filtration net 8 and left extruding pipe 4 are extruded the liquid of small-particle throw out and random quantity, discard it;
3, rock main body 1, after its content is mixed, right-hand member left standstill 10 minutes or placed whizzer low speed (500～2000 rev/mins) to rotate down 2 minutes again, open following right aling cap 7 afterwards, manual pinching main body 1, the liquid of solids precipitation thing and random quantity during process coarse filtration net 9 and right extruding pipe 5 are extruded---be the extract that contains the major diameter cell, can be directly used in and make sample, measuring; By macrobead and the liquid that coarse filtration net 9 is held back, discard it.
Indicate:
Macrobead in the literary composition: refer to that diameter surpasses the cell mass of 70um, impurity cluster.
Particle in the literary composition: refer to diameter 20 to the major diameter cell of 60um.
Literary composition small particles: refer to all kinds of minor diameter cells of diameter below 20um, cell debris, chaff interferences such as impurity.
Claims (3)
1. the cell specimen screen selecting and separating mechanism has filter screen, it is characterized in that: have the clamshell type cylindrical body that can be screwed onto together, the left and right two ends of main body have circle or the conical head that processes with main body integral body respectively; Has an extruding pipe that is communicated with and processes with circle or conical head integral body with the tube chamber of main body on the top of each circle or conical head respectively; Also have with each circle or conical head together with the supporting corresponding capping of the extruding pipe on it, what capping can detachable property is contained on the main body; Two ends in main body are bordering on the inwall at the position that is connected with each circle or conical head, have the filtering net setting-in mechanism of circumferential laying respectively, and thin, coarse filtration net is inlaid in respectively in the filtering net setting-in mechanism.
2. cell specimen screen selecting and separating mechanism according to claim 1, it is characterized in that: left and right capping (6, the 7) structure that is contained on the main body (1) of detachable property respectively is the type of being spirally connected, have internal thread (12) on the port inner wall of left and right capping (6,7), have on the corresponding outer wall of main body (1) and the supporting corresponding outside screw of internal thread (12) (13).
3. cell specimen screen selecting and separating mechanism according to claim 1 and 2, it is characterized in that: the structure of filter screen setting-in mechanism (11) is the circumferential recess formula, two ends in main body (1) are bordering on the inwall at the position that is connected with left and right circle or conical head (2,3), has the twice convex formula circumferential hoop (14) that processes with main body (1) integral body, has circumferential recess (15) therebetween, the internal diameter Φ of the internal diameter of circumferential recess (15) and main body (1)
0Equate its width m
0Be 1.5～2mm; The height h of convex formula circumferential hoop (14) and width m
1Be 1.5～2mm; Perhaps the structure of filter screen setting-in mechanism (11) is circumferential slit type, two ends and a left side in main body (1), right circle or conical head (2,3) be bordering on the inwall at position of connection, has the circumferential crack of being formed by one convex formula circumferential hoop (14) and circumferentially uniform interior raised line (16) (17), convex formula circumferential hoop (14) is positioned at by circular head one side, circumferentially the specification of the circumferential recess (15) in the corresponding specification of crack (17) and the described circumferential recess formula structure is identical, the height of interior raised line (16) and width are identical with the corresponding size of convex formula circumferential hoop (14), and interior raised line (16) is four～eight of uniform numbers circumferentially.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310242528.5A CN103275865B (en) | 2013-06-19 | 2013-06-19 | Cell specimen screening and separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310242528.5A CN103275865B (en) | 2013-06-19 | 2013-06-19 | Cell specimen screening and separation device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103275865A true CN103275865A (en) | 2013-09-04 |
| CN103275865B CN103275865B (en) | 2015-06-10 |
Family
ID=49058492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310242528.5A Active CN103275865B (en) | 2013-06-19 | 2013-06-19 | Cell specimen screening and separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103275865B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2096761U (en) * | 1991-03-21 | 1992-02-19 | 韩建礼 | Gradient seperating test tube |
| CN1560223A (en) * | 2004-02-17 | 2005-01-05 | 东南大学 | Filter for cell |
| DE69833488D1 (en) * | 1997-06-10 | 2006-04-20 | Bruce J Bradley | DEVICE FOR MICROBIAL SAMPLING AND ENRICHMENT |
| CN2892868Y (en) * | 2005-12-06 | 2007-04-25 | 刘瑞东 | Cell detaching tube |
| CN200940146Y (en) * | 2006-08-18 | 2007-08-29 | 王茜 | Sieve like large intestine cells collector |
| CN202214356U (en) * | 2011-10-09 | 2012-05-09 | 黑龙江八一农垦大学 | Hemopoietic stem cell separator |
| CN202415550U (en) * | 2011-12-23 | 2012-09-05 | 北京青藤谷禧干细胞科技研究院有限公司 | Strainer-contained transfer pipette for cell separation |
| CN203307334U (en) * | 2013-06-19 | 2013-11-27 | 夏炜 | Cell specimen screening and separating device |
-
2013
- 2013-06-19 CN CN201310242528.5A patent/CN103275865B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2096761U (en) * | 1991-03-21 | 1992-02-19 | 韩建礼 | Gradient seperating test tube |
| DE69833488D1 (en) * | 1997-06-10 | 2006-04-20 | Bruce J Bradley | DEVICE FOR MICROBIAL SAMPLING AND ENRICHMENT |
| CN1560223A (en) * | 2004-02-17 | 2005-01-05 | 东南大学 | Filter for cell |
| CN2892868Y (en) * | 2005-12-06 | 2007-04-25 | 刘瑞东 | Cell detaching tube |
| CN200940146Y (en) * | 2006-08-18 | 2007-08-29 | 王茜 | Sieve like large intestine cells collector |
| CN202214356U (en) * | 2011-10-09 | 2012-05-09 | 黑龙江八一农垦大学 | Hemopoietic stem cell separator |
| CN202415550U (en) * | 2011-12-23 | 2012-09-05 | 北京青藤谷禧干细胞科技研究院有限公司 | Strainer-contained transfer pipette for cell separation |
| CN203307334U (en) * | 2013-06-19 | 2013-11-27 | 夏炜 | Cell specimen screening and separating device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103275865B (en) | 2015-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN203745435U (en) | Special sleeve centrifugal QuEChERS purifier | |
| JPH03500003A (en) | filter unit | |
| CN101813582A (en) | Integrated pesticide residue detection pretreatment device and pretreatment method | |
| CN201193997Y (en) | Cellular fluid processing centrifugal device | |
| CN109342162A (en) | A kind of sample extraction separator and sample processing device | |
| CN103674668B (en) | Pollutant online pre-separation enriching apparatus in a kind of water sample with bulk mass | |
| CN204043972U (en) | A kind of to granulated samples carrying out the chemical detection liquid extraction tube of lixiviate | |
| WO2018161744A1 (en) | Filter apparatus and application thereof to sample pretreatment in food safety testing | |
| CN203307334U (en) | Cell specimen screening and separating device | |
| CN204710574U (en) | A kind of fluid separation applications centrifuge tube | |
| CN101362120B (en) | Large-capacity continuous centrifugal separation device | |
| CN103275865B (en) | Cell specimen screening and separation device | |
| CN209231073U (en) | A kind of dissolved organic matter extraction integrated apparatus | |
| CN210198828U (en) | Full-automatic QuEChERS pretreatment all-in-one machine based on magnetic separation | |
| US10092911B2 (en) | Centrifuge filter tube | |
| CN211069216U (en) | Multi-connected vacuum suction filter | |
| CN210543745U (en) | Sample purification device for drug residue analysis | |
| CN204638219U (en) | A kind of filtration pipettor | |
| CN109490935B (en) | Filtering material sample gamma measurement source preparation method and device based on particulate matter extraction | |
| CN109540618B (en) | Method, device and system for separating and extracting particles from filter material substrate | |
| CN206321612U (en) | A kind of filter-type chromatogram bottle and chromatograph | |
| JP2006280318A (en) | Separating and recovering method | |
| CN201032478Y (en) | Liquid based thin layer cell processor | |
| KR101629902B1 (en) | Apparatus for Filtering Biomass from Groundwater and Surfacewater | |
| CN205642979U (en) | Processing apparatus before extraction structure and liquid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20160202 Address after: 100000 No. 25 hospital, South Third Ring Road, Fengtai District, Beijing 14, 309 Patentee after: Beijing Chengzhi Guanghui Technology Co., Ltd. Address before: 100086 Beijing Chaoyang District City, St. hope earth C building room 0306 Patentee before: Xia Wei |