CN103274919A - Extraction method of peony overground part - Google Patents

Abstract

The invention discloses an extraction method of a peony overground part. The method comprises the following steps of crushing peony overground part stem and leaf as raw materials into coarse powder, adding water 5-8 times that of the raw materials, adding vinegar to adjust the PH to be 5-6, decocting and filtering to obtain an extracting solution and concentrating into an extract; adding 50-60% ethanol solution with the weight 3-4 times that of filtering residue to filtering residue, adding salt with the weight 1-2% that of filtering residue and sodium bicarbonate with the weight 3-4% that of filtering residue, carrying out ultrasonic-assisted extraction, filtering to obtain a filter liquor and concentrating so as to obtain an extract; and refining the extract. By using the extract obtained by extracting the peony overground part, the content of paeonol in the refined extractive is 70%.

Description

Tree peony over-ground part extracting method

Technical field

The invention belongs to the Chinese medicinal materials extractive technique, specifically is a kind of tree peony over-ground part extracting method.

Background technology

Tree Peony Bark is ranunculaceae peony Paeonia suffruticosaAndr. dry root skin.Has clearing heat and cooling blood, effect such as promoting blood circulation and removing blood stasis.Be used for heat and go into ying blood, maculae caused by violent heat pathogen, hematemesis and epistaxis, night fever abating at dawn, lossless hectic fever due to yin is pounced on the pain of injury, carbuncle sore tumefacting virus through closing dysmenorrhoea, falling.

Paeonol is the Tree Peony Bark main active ingredient, and Paeonol (Paeonal) has another name called paeonol, Chinese herbaceous peony phenol, and chemistry " 2 hydroxyls, 4 methoxyacetophenones " by name is colourless needle crystal, is dissolved in ethanol, ether, acetone, chloroform, benzene and dithiocarbonic anhydride.The Paeonol fusing point is 50 ℃.Paeonol has multiple pharmacological effect such as analgesia, hemostasis, anti-inflammatory, antibiotic, step-down, and Paeonal injection agent at present is clinical main formulation, pain disease with Dermatology Department is clinical is used widely.Simultaneously Paeonol can influence mechanism such as tumour cell genetic expression generation kills and wounds, induces differentiation and effects such as apoptosis, inhibition invasion and attack and transfer to tumour cell by suppressing tumor cell proliferation, inducing tumor cell differentiation and apoptosis, and its prospect in medicine is quite extensive.Demand to Paeonol is bigger in the market, except being used for the pharmaceutical preparation raw material, now also is widely used in the starting material of daily chemical products such as toothpaste and hair care, skin care, beauty treatment.

Tree peony all has cultivation on a large scale throughout the country, and paeonol content is all variant in the different sites, and is higher with the content of root skin, the root skin〉root〉stem skin＞leaf〉stem.Modern people mainly extract Paeonol from Tree Peony Bark, economic benefit is considerable, are new research directions and extract Paeonol from the tree peony over-ground part.

Summary of the invention

The purpose of this invention is to provide a kind of tree peony over-ground part extracting method.

Technical solution of the present invention is as follows:

Tree peony over-ground part extracting method is characterized in that may further comprise the steps:

(1), be that raw material powder is broken into thick end with tree peony over-ground part cauline leaf, add 5-8 times of water gaging, adding vinegar, to transfer PH be 5-6, decocts, and filters, and obtains extracting solution and be condensed into medicinal extract;

(2), filter residue adds 3-4 and doubly measures the 50-60% ethanolic soln, add again and be equivalent to the salt of filter residue weight 1-2%, the sodium bicarbonate of 3-4%, ultrasonic-assisted extraction, concentrated medicinal extract filters to get filtrate;

(3), merge medicinal extract, water dissolution removes slag;

(4), lysate is by macroporous adsorptive resins, water, 2-3% salt solution, the removal of impurities of 2-3% sodium hydrogen carbonate solution wash-out successively;

(5), again with 30-70% aqueous ethanolic solution wash-out, collect elutriant, the elutriant concentrate drying gets refining extract.

The result of experimental study and meaning:

1, extract the resulting medicinal extract of tree peony over-ground part by this experiment, paeonol content is 70% in the refining extract.

When 2, gathering Tree Peony Bark, agents area is underground part, and over-ground part has generally burnt when bavin, even just throwing away in the ground of having.Paeonol in the tree peony over-ground part is extracted, can be described as refuse reclamation, effectively reclaimed waste resource, in reality is produced, extremely important meaning is arranged.

3, adopt this extraction process simple to operate, extracts active ingredients is many, is the best approach that the tree peony over-ground part extracts, and resulting product purity is higher, so this extraction process provides foundation for the production of extracting Paeonol in the tree peony over-ground part.

Embodiment

Tree peony over-ground part extracting method is characterized in that may further comprise the steps:

(1), be that raw material powder is broken into thick end with tree peony over-ground part cauline leaf, add 8 times of water gagings, adding vinegar, to transfer PH be 5, decocts, and filters, and obtains extracting solution and be condensed into medicinal extract;

(2), filter residue adds 4 times of amount 50% ethanolic solns, add again and be equivalent to the salt of filter residue weight 2%, 3% sodium bicarbonate, ultrasonic-assisted extraction, concentrated medicinal extract filters to get filtrate;

(3), merge medicinal extract, water dissolution removes slag;

(4), lysate is by macroporous adsorptive resins, water, 3% salt solution, 3% sodium hydrogen carbonate solution wash-out removal of impurities successively;

(5), again with 30-70% aqueous ethanolic solution gradient elution, collect elutriant, the elutriant concentrate drying gets refining extract.

Paeonol content is 70% in the refining extract.Be equivalent to 2% of raw material weight.

Claims (1)

1. tree peony over-ground part extracting method is characterized in that may further comprise the steps:

(1), be that raw material powder is broken into thick end with tree peony over-ground part cauline leaf, add 5-8 times of water gaging, adding vinegar, to transfer PH be 5-6, decocts, and filters, and obtains extracting solution and be condensed into medicinal extract;

(2), filter residue adds 3-4 and doubly measures the 50-60% ethanolic soln, add again and be equivalent to the salt of filter residue weight 1-2%, the sodium bicarbonate of 3-4%, ultrasonic-assisted extraction, concentrated medicinal extract filters to get filtrate;

(3), merge medicinal extract, water dissolution removes slag;

(4), lysate is by macroporous adsorptive resins, water, 2-3% salt solution, the removal of impurities of 2-3% sodium hydrogen carbonate solution wash-out successively;

(5), again with 30-70% aqueous ethanolic solution wash-out, collect elutriant, the elutriant concentrate drying gets refining extract.