CN103251757B - Borneol-containing compound preparation as well as preparation method and application thereof - Google Patents
Borneol-containing compound preparation as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses an application of a borneol-containing compound preparation in preparation of medicines for treating depression. The traditional Chinese medicine compound preparation is prepared from the following raw material medicines in parts by weight: 13-17 parts of radix bupleuri, 13-17 parts of immature bitter orange, 10-14 parts of Ligusticum wallichii, 8-12 parts of Codonopsis pilosula, 8-12 parts of Rhizoma Atractylodis Macrocephalae, 10-14 parts of Poria cocos, 8-12 parts of Mangnolia officinalis, 5-7 parts of rhizoma pinelliae preparata, 15-25 parts of nacre mother of pearl, 6-10 parts of fiveleaf akebia fruit, 1-3 parts of borneol and 4-8 parts of liquorice. A preparation method is further disclosed. The pharmacological testing result shows that the preparation has the improving effect on behavioristics of depression, and the action is associated with factors which adjust related nerves in the brain. The preparation provided by the invention has the advantages that the process of the compound preparation is simple to control, the bioavailability is high, the effect is remarkable and the cost is low.
Description
Technical field
The present invention relates to technical field of Chinese medicines, be specifically related to compound Chinese medicinal preparation of a kind of Cure of depression and its preparation method and application.
Background technology
Depression is a kind of to continue the depressed affective disorder disease as cardinal symptom, is modal one in mental sickness.Its pathogenesis complexity, relevant with the various factors such as heredity, environment, society, along with social development, the pressure competition that the work of people's daily life is faced increasingly sharpens, depression have the trend increasing gradually, its sickness rate is soaring just fast.WHO prediction will be number two to the year two thousand twenty depression in total burden of disease.Exceed 2,600 ten thousand people and suffer from depression China is existing, wherein approximately 10%～15% patients with depression is died from suicide.The control of depression has been become to current vital task.
In the medicine of Cure of depression, chemicals curative effect is obvious, but after such medicine long-term taking, its side effect is troubling all the time, as the toxic and side effects of tricyclic antidepressant to heart, and body weight change and sexual dysfunction that other chemicalses cause are not resolved always, also exist the shortcomings such as antidepressant spectrum is narrow, easy recurrence simultaneously, thus action temperature and, the Chinese medicine of few side effects and prescription thereof more and more receive people's concern.
Summary of the invention
Goal of the invention: in order to address the above problem, the object of the present invention is to provide compound Chinese medicinal preparation of a kind of Cure of depression and its preparation method and application.
Technical scheme: the object of the invention is to realize by following scheme:
A compound Chinese medicinal preparation for Cure of depression, it is prepared by the raw material of following parts by weight: Radix Bupleuri 13-17 weight portion, Fructus Aurantii Immaturus 13-17 weight portion, Rhizoma Chuanxiong 10-14 weight portion, Radix Codonopsis 8-12 weight portion, Rhizoma Atractylodis Macrocephalae 8-12 weight portion, Poria 10-14 weight portion, Cortex Magnoliae Officinalis 8-12 weight portion, Rhizoma Pinelliae 5-7 weight portion, Concha Margaritifera 15-25 weight portion, Fructus Akebiae 6-10 weight portion, Borneolum Syntheticum 1-3 weight portion, Radix Glycyrrhizae 4-8 weight portion.
The compound Chinese medicinal preparation of above-mentioned Cure of depression, it is prepared by the raw material of following parts by weight: Radix Bupleuri 15 weight portions, Fructus Aurantii Immaturus 15 weight portions, Rhizoma Chuanxiong 12 weight portions, Radix Codonopsis 10 weight portions, the Rhizoma Atractylodis Macrocephalae 10 weight portions, Poria 12 weight portions, Cortex Magnoliae Officinalis 10 weight portions, Rhizoma Pinelliae 6 weight portions, Concha Margaritifera 20 weight portions, Fructus Akebiae 8 weight portions, Borneolum Syntheticum 2 weight portions, Radix Glycyrrhizae 6 weight portions.
The compound Chinese medicinal preparation of above-mentioned Cure of depression, preparation method is: first Concha Margaritifera is pulverized, cross 60 mesh sieves, add 10 times of water yields, be decocted first after 20 minutes, again by Radix Bupleuri, Fructus Aurantii Immaturus, Rhizoma Chuanxiong, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria, Cortex Magnoliae Officinalis, Rhizoma Pinelliae, Fructus Akebiae, Radix Glycyrrhizae, with 12 times of medical material weight amount distilled water extraction 2-3 time, each 1-2 hour, filtered through gauze, merging filtrate, the extractum that while being evaporated to 65 DEG C, relative density is 1.15-1.20, add ethanol and make to reach 75-85% containing the percent by volume of alcohol amount, low-temperature vacuum drying 12h, a gets dry extract, separately 70% dissolve with ethanol by 10 times of amounts by Borneolum Syntheticum, beta-schardinger dextrin-is added in 40 DEG C of distilled water and makes saturated solution, the weight of beta-schardinger dextrin-is 7 times of Borneolum Syntheticum weight, under turn 800/min stirring condition, slowly drip the above-mentioned Borneolum Syntheticum with dissolve with ethanol, stir 1h, then put cold preservation 24h in refrigerator, filter, and wash away the not beta-schardinger dextrin-of enclose of part with a small amount of distilled water, the low-temperature vacuum drying 24h b that gets dry extract, dry cream a and dry cream b are pulverized, mix, add dextrin and 70% ethanol soft material processed, rollover is granulated, gained granule is crossed 65 mesh sieves, subpackage makes granule.
The compound Chinese medicinal preparation of above-mentioned Cure of depression is in the application of preparing in Cure of depression medicine.
Beneficial effect:
1, the traditional Chinese medical science thinks that depression and liver, spleen, cardiac function are not normal relevant.Liver is lost bar and reaches, functional activity of QI being not smooth, so that anxiety and rage, depression of liver-QI.In addition sorrow is pondered over, and psychentonia also can make stagnation of spleen-QI, causes dysfunction of the spleen in transportation.And the Nervous and Mental Factors such as worried grief are damaged the mind, cause emotional stress, the heart is become homeless foster.Therefore the main rule for the treatment of that depressed liver-energy dispersing and QI regulating resolving depression is depression, in addition depression also usually adopt respectively with blood stasis, expectorant knot, humidity hysteresis etc. invigorate blood circulation, reduce phlegm, the method such as damp eliminating.Radix Bupleuri depressed liver-energy dispersing and function of gallbladder promoting, thin gas resolving depression, loose fire in pharmaceutical composition of the present invention, the heap soil or fertilizer over and around the roots of joining Fructus Aurantii Immaturus purgation of spleen excess gas is stagnant, and in tune, Jiao's motion and Radix Bupleuri are with using one rise and one drop, and the merit of reinforcement depressed liver-energy dispersing and QI regulating, to reach strongly fragrant heresy, is monarch drug altogether.Rhizoma Chuanxiong activating QI to alleviate the depression, promoting blood circulation and stopping pain; Radix Codonopsis can spleen reinforcing lung gas, be equal to use with the Rhizoma Atractylodis Macrocephalae, Poria, play altogether the effect of QI invigorating, spleen invigorating, dehumidifying.Cortex Magnoliae Officinalis, Rhizoma Pinelliae are led strongly fragrant gas, the arresting convulsion of Concha Margaritifera relieving palpitation, liver heat removing relieving restlessness, heat-clearing and toxic substances removing off so that the chest stuffiness relieving is unimpeded; Fructus Akebiae liver-smoothing, qi-regulating, promoting blood circulation and stopping pain, relieving restlessness diuresis, supplement to the Herbal record " a surname is logical, goes dysphoria with smothery sensation, and making us of food is not lend oneself to worry and anxiety ".This eight tastes medicine compatibility uses, and the effect that plays altogether resolving depression blood stasis dispelling, reduces phlegm and dehumidify, is ministerial drug altogether.Borneolum Syntheticum is apt to away alter and is had one's ideas straightened out, and does not reach without past, and the gas of fragrance, can ward off all evilnesses, hot property, all rheumatism of faling apart.Radix Glycyrrhizae is is unclogged and readjusted irritability, and coordinating the actions of various ingredients in a prescription, altogether for assistant makes.All medicines share, and play altogether dispersing the stagnated live-QI to relieve the stagnation of QI, circulation of qi promoting, the effect of promoting blood circulation and stopping pain.
2, Formulation of the present invention is reasonable, and compatibility is rigorous, through long-term clinical verification, determined curative effect.
3, the present invention consists of Chinese crude drug, have no side effect, and low price.
Detailed description of the invention
Form by the following examples, foregoing of the present invention is described in further detail again, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to following example, all technology realizing based on foregoing of the present invention all belong to scope of the present invention.
1,, in conjunction with detailed description of the invention, the present invention is further described as follows:
Fructus Akebiae in the present invention is the ripe dry fruit of plants of Lardizabalaceae Caulis Akebiae, and main component is saponin and fatty oil, not containing Aristolochic Acid.Other medical material is selected pharmacopeia kind.
Embodiment 1: get Radix Bupleuri 15g, Fructus Aurantii Immaturus 15g, Rhizoma Chuanxiong 12g, Radix Codonopsis 10g, Rhizoma Atractylodis Macrocephalae 10g, Poria 12g, Cortex Magnoliae Officinalis 10g, Rhizoma Pinelliae 6g, Concha Margaritifera 20g, Fructus Akebiae 8g, Borneolum Syntheticum 2g, Radix Glycyrrhizae 6g.First Concha Margaritifera is pulverized, crossed 60 mesh sieves, 200ml adds water, be decocted first after 20 minutes, by Radix Bupleuri, Fructus Aurantii Immaturus, Rhizoma Chuanxiong, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria, Cortex Magnoliae Officinalis, Rhizoma Pinelliae, Fructus Akebiae, Radix Glycyrrhizae, use 12 times of medical material weight to measure distilled water extraction 2 times, each 1 hour again, filtered through gauze, merging filtrate, the extractum that while being evaporated to 65 DEG C, relative density is 1.15, adds ethanol and makes to reach 75% containing the percent by volume of alcohol amount, low-temperature vacuum drying 12h, a gets dry extract, separately 70% dissolve with ethanol by 10 times of amounts by Borneolum Syntheticum, beta-schardinger dextrin-is added in 40 DEG C of distilled water and makes saturated solution, the weight of beta-schardinger dextrin-is 7 times of Borneolum Syntheticum weight, under turn 800/min stirring condition, slowly drip the above-mentioned Borneolum Syntheticum with dissolve with ethanol, stir 1h, then put cold preservation 24h in refrigerator, filter, and wash away the not beta-schardinger dextrin-of enclose of part with a small amount of distilled water, the low-temperature vacuum drying 24h b that gets dry extract, dry cream a and dry cream b are pulverized, mix, add dextrin and 70% ethanol soft material processed, rollover is granulated, gained granule is crossed 65 mesh sieves, subpackage makes granule.
Embodiment 2: get Radix Bupleuri 17g, Fructus Aurantii Immaturus 17g, Rhizoma Chuanxiong 14g, Radix Codonopsis 12g, Rhizoma Atractylodis Macrocephalae 12g, Poria 14g, Cortex Magnoliae Officinalis 12g, Rhizoma Pinelliae 7g, Concha Margaritifera 25g, Fructus Akebiae 10g, Borneolum Syntheticum 3g, Radix Glycyrrhizae 8g.First Concha Margaritifera is pulverized, crossed 60 mesh sieves, 250ml adds water, be decocted first after 20 minutes, by Radix Bupleuri, Fructus Aurantii Immaturus, Rhizoma Chuanxiong, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria, Cortex Magnoliae Officinalis, Rhizoma Pinelliae, Fructus Akebiae, Radix Glycyrrhizae, use 12 times of medical material weight to measure distilled water extraction 3 times, each 2 hours again, filtered through gauze, merging filtrate, the extractum that while being evaporated to 65 DEG C, relative density is 1.20, adds ethanol and makes to reach 85% containing the percent by volume of alcohol amount, low-temperature vacuum drying 12h, a gets dry extract, separately 70% dissolve with ethanol by 10 times of amounts by Borneolum Syntheticum, beta-schardinger dextrin-is added in 40 DEG C of distilled water and makes saturated solution, the weight of beta-schardinger dextrin-is 7 times of Borneolum Syntheticum weight, under turn 800/min stirring condition, slowly drip the above-mentioned Borneolum Syntheticum with dissolve with ethanol, stir 1h, then put cold preservation 24h in refrigerator, filter, and wash away the not beta-schardinger dextrin-of enclose of part with a small amount of distilled water, the low-temperature vacuum drying 24h b that gets dry extract, dry cream a and dry cream b are pulverized, mix, add dextrin and 70% ethanol soft material processed, rollover is granulated, gained granule is crossed 65 mesh sieves, subpackage makes granule.
Embodiment 3: get Radix Bupleuri 13g, Fructus Aurantii Immaturus 13g, Rhizoma Chuanxiong 10g, Radix Codonopsis 8g, Rhizoma Atractylodis Macrocephalae 8g, Poria 10g, Cortex Magnoliae Officinalis 8g, Rhizoma Pinelliae 5g, Concha Margaritifera 15g, Fructus Akebiae 6g, Borneolum Syntheticum 1g, Radix Glycyrrhizae 4g, first Concha Margaritifera is pulverized, cross 60 mesh sieves, 150ml adds water, be decocted first after 20 minutes, again by Radix Bupleuri, Fructus Aurantii Immaturus, Rhizoma Chuanxiong, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria, Cortex Magnoliae Officinalis, Rhizoma Pinelliae, Fructus Akebiae, Radix Glycyrrhizae, with 12 times of medical material weight amount distilled water extraction 2 times, each 1 hour, filtered through gauze, merging filtrate, the extractum that while being evaporated to 65 DEG C, relative density is 1.15, adding ethanol makes to reach 75% containing the percent by volume of alcohol amount, low-temperature vacuum drying 12h, a gets dry extract, separately 70% dissolve with ethanol by 10 times of amounts by Borneolum Syntheticum, beta-schardinger dextrin-is added in 40 DEG C of distilled water and makes saturated solution, the weight of beta-schardinger dextrin-is 7 times of Borneolum Syntheticum weight, under turn 800/min stirring condition, slowly drip the above-mentioned Borneolum Syntheticum with dissolve with ethanol, stir 1h, then put cold preservation 24h in refrigerator, filter, and wash away the not beta-schardinger dextrin-of enclose of part with a small amount of distilled water, the low-temperature vacuum drying 24h b that gets dry extract, dry cream a and dry cream b are pulverized, mix, add dextrin and 70% ethanol soft material processed, rollover is granulated, gained granule is crossed 65 mesh sieves, subpackage makes granule.
2. the experimental animal model pharmacodynamic study of pair depression
Laboratory animal: male clean level SD rat, body weight 180-220g, Zhejiang University's Experimental Animal Center provides, production licence: [SCXK (Zhejiang) 2008-0033], and freely ingest and drink water, in experimental situation, adapt to test after 3d.
Experimental agents: the preparation method preparation by embodiment 1: get Radix Bupleuri 15g, Fructus Aurantii Immaturus 15g, Rhizoma Chuanxiong 12g, Radix Codonopsis 10g, Rhizoma Atractylodis Macrocephalae 10g, Poria 12g, Cortex Magnoliae Officinalis 10g, Rhizoma Pinelliae 6g, Concha Margaritifera 20g, Fructus Akebiae 8g, Borneolum Syntheticum 2g, Radix Glycyrrhizae 6g.First Concha Margaritifera is pulverized, crossed 60 mesh sieves, 200ml adds water, be decocted first after 20 minutes, by Radix Bupleuri, Fructus Aurantii Immaturus, Rhizoma Chuanxiong, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria, Cortex Magnoliae Officinalis, Rhizoma Pinelliae, Fructus Akebiae, Radix Glycyrrhizae, use 12 times of medical material weight to measure distilled water extraction 2 times, each 1 hour again, filtered through gauze, merging filtrate, the extractum that while being evaporated to 65 DEG C, relative density is 1.15, adds ethanol and makes to reach 75% containing the percent by volume of alcohol amount, low-temperature vacuum drying 12h, a gets dry extract, separately 70% dissolve with ethanol by 10 times of amounts by Borneolum Syntheticum, beta-schardinger dextrin-is added in 40 DEG C of distilled water and makes saturated solution, the weight of beta-schardinger dextrin-is 7 times of Borneolum Syntheticum weight, under turn 800/min stirring condition, slowly drip the above-mentioned Borneolum Syntheticum with dissolve with ethanol, stir 1h, then put cold preservation 24h in refrigerator, filter, and wash away the not beta-schardinger dextrin-of enclose of part with a small amount of distilled water, the low-temperature vacuum drying 24h b that gets dry extract, dry cream a and dry cream b are pulverized, mix, add dextrin and 70% ethanol soft material processed, rollover is granulated, gained granule is crossed 65 mesh sieves, subpackage makes granule.Every bag of granule is 10g, is wherein 42g containing crude drug amount.
Dosage: according to " the dose,equivalent ratio table of converting by surface between humans and animals ", consumption with reference to SD rat converts, every day, 60 kilograms of people's consumption was 126g crude drug amount, every day, the consumption of SD rat of every 200g was 126 ÷ 60 × 5 × 0.2=2.1g crude drug amounts, because every bag of granule is 10g, be wherein 42g containing crude drug amount, the consumption that is every SD rat is 0.50g granule, therefore determine every gavage 0.50g granule of low dosage, middle dosage and high dose be gavage 1.0g and 2.0g respectively, every day, the granule of SD rat oral gavage of every 200g was respectively 2.1g containing crude drug amount, 4.2g, 8.4g, by SD rat body weight, low dosage, middle dosage and high dose are respectively 10.5g crude drug amount/kg, 21.0g crude drug amount/kg and 42.0g crude drug amount/kg.
Positive drug paroxetine, GlaxoSmithKline PLC company product, lot number 1210023, specification is 20mgx7 sheet/box, and patient uses general each 1, every day 1 time, be people's consumption per day 20mg, the SD rat consumption that is equivalent to every 200g is 20 ÷ 60 × 5 × 0.2=0.33mg, calculates by per kilogram SD rat, and positive drug group gives acyclovir 1.65mg/kg.
Instrument and reagent: YSD-4G type electronic stimulator (Bangbu radio two factories), Morris water maze (Anhui Zhenghua Biology Instrument Equipment Co., Ltd.), Any-maze animal behavior analytical system (Stoeling company of the U.S.).
2.1 rat modeling and group technologies
The method of pressing Willner P. etc. slightly row is improved.Raise to dwindle activity space with little mouse cage is independent, accept simultaneously 21 days chronic moderate strength stress: by animal press from both sides at random weekly tail 3min, prohibit water 24h, fasting 24h, put upside down round the clock 24h, 160Hz level vibration 5min, electronic stimulator electric shock (30v, electric shock 5s, interval 5s, carries out 2min altogether), 4 DEG C of water swimming 5min.By aforesaid way respectively repeatedly 3 times, totally 21 kinds of stimulating methods are included random form in, and in 21 days, bestow at random a kind of stimulation every day.Modeling rat is divided into model group, paroxetine group, Chinese traditional compound medicine I group (10.5g crude drug amount/kg), Chinese traditional compound medicine II group (21g crude drug amount/kg), Chinese traditional compound medicine III group (42g crude drug amount/kg) at random, totally 5 groups, 10 every group.10 not modelings of normal rats, normally raise 21.
2.2 drug treating methods
Start to carry out administration processing according to grouping situation, every day 1 time, altogether 14d.Except normal group, other animal in every day same time insert 20min in holder, 14d continuously.After 14d for following detection.
2.3 indexs detect
2.3.1 behavioristics measures
2.3.2 sucrose water consumption and body weight determination
Before experiment, animal training is drunk sucrose solution.Prohibit after water 24h the 1% sucrose water that every rat gives 20ml, after 1h, take water bottle away and measure residual volume, obtain sucrose solution and consume volume (ml), weigh to rat simultaneously.
2.3.3 Open-field (OFT) open field test
The spacious case of self-control uncovered, bottom surface is 64 and waits lattice (10cm × 10cm).Rat is put into center grid, timing 3min, the grid number that while getting over using rat, extremity all enter, as horizontal movement number of times, soars or seeks connections with tank wall, rear number of times for the number of times that moves both vertically so that rat forelimb performance is liftoff.
2.3.4 Morris water maze laboratory
Morris water maze is made up of a rustless steel round pool and a platform, and diameter is 100cm, depth of water 50cm.First in pond, inject clear water, then add a little prepared Chinese ink to make water blackening, water temperature is controlled at (22 ± 2) DEG C.Pond is divided into 2 regions.Being less than 50cm with platform distance is nearly platform district, and being greater than 50cm with platform distance is platform far away district.The peacetime training time is set 70s as the maximum incubation period of escaping on platform, exceedes 70s and directly rat is drawn to platform.When experiment, rat is put into arbitrary position at random, to find time of platform as incubation period, to find the track swum across of platform as total distance, utilize Any-maze animal behavior analytical system to observe rat incubation period, total distance, swimming rate, nearly platform district track distance, platform far away district track distance, near/platform far away district distance ratio, nearly platform district's time, platform district's time far away, near/platform far away district time ratio.
2.3.5 the detection that nerve growth factor (NGF), Brain Derived Neurotrophic Factor (BDNF) are expressed
Put to death rat, separate frontal cortex and Hippocampus after getting brain, 10% formaldehyde is fixed, and by Immunohistochemical Method, observes the expression of Zhe Lianggenao district NGF and BDNF.The specialty picture analyzing software I mage-Pro Plus of Media Cybernetics company of the application U.S. 6.0 carries out picture analyzing.
2.4 statistical method
Adopt SPSS 12.0 statistical softwares to carry out one factor analysis of variance, mean scholar standard deviation for data
represent, P < 0.05 has statistical significance for difference.
2.5 experimental result
2.5.1 the impact on the water consumption of depression rat sucrose
With the comparison of blank group, the visible body weight of model group rat obviously alleviates, and sucrose solution consumption significantly reduces (P < 0.01).Give after paroxetine, visible volume representation work increases, and obvious increase (with model group comparison, P < 0.05) is also shown in sucrose solution consumption.Three visible body weight of dosage group of Chinese traditional compound medicine and sucrose solution consume improve (with the model group comparison, P < 0.01) of volume, and visible dose dependent.And the effect of Chinese traditional compound medicine III group is better than positive drug paroxetine group.The results are shown in Table 1.
The impact of table 1 on depression rat body weight and sucrose water consumption (
n=10)
With model group comparison,
*p < 0.05,
*p < 0.01.
2.5.2 the impact on the activity of depression rat prologue
With the comparison of blank group, the obvious minimizing (P < 0.01) of horizontal and vertical motion in prologue activity as seen of model group rat.Give after paroxetine all visible significantly increases (with model group comparison, P < 0.05) no matter horizontal movement still moves both vertically.Also the improving of prologue activity (with model group comparison, P < 0.05,0.01) as seen of three dosage groups of Chinese traditional compound medicine, and visible dose dependent.And the effect of Chinese traditional compound medicine III group is better than positive drug paroxetine group, the results are shown in Table 2.
The impact of table 2 on the activity of depression rat prologue (
n=10)
With model group comparison,
*p < 0.05,
*p < 0.01.
2.5.3 on the ethological impact of depression rat Morris water maze
With the comparison of blank group, the remarkable increase of test middle reaches Swimming Association of the visible Morris water maze of model group rat behavioristics distance, nearly platform district track distance, platform far away district track distance, the incubation period of appearing on the stage, close-table district time and far from the table district time, closely/platform district's time far away is than significantly reducing, (P < 0.05,0.01).Give after paroxetine, (with model group comparison, P < 0.05,0.01) significantly shortened in total distance of swimming as seen, platform far away district distance, platform far away district distance, the incubation period of appearing on the stage, close-table district time and platform far away district the time.Significantly shorten (with model group comparison the time in the total distance of the visible swimming of three dosage groups, platform far away district distance, platform far away district distance, the incubation period of appearing on the stage, close-table district time and the platform far away district of Chinese traditional compound medicine, P < 0.05,0.01), in addition be also shown in closely/platform far away of high dose group district time scale significantly increases (with model group comparison, P < 0.05), this point is better than paroxetine group.The results are shown in Table 3-table 4.
The total distance of table 3 each group rat, average speed, nearly platform district track distance, platform district's track distance far away and nearly platform district's distance ratio result comparison
(n=10)
With model group comparison,
*p < 0.05,
*p < 0.01.
The each group of table 4 rat incubation period, nearly platform district's time, platform district's time far away and nearly platform district's time scale result comparison
(n=10)
With model group comparison,
*p < 0.05,
*p < 0.01.
2.5.4 the impact on depression rat frontal cortex and hippocampus NGF, BDNF expression
With the comparison of blank group, the visible NGF of model group rat and BDNF are in the remarkable minimizing (P < 0.01) of frontal cortex and hippocampus expression.Give after paroxetine the remarkable increase (with model group comparison, P < 0.05) of visible these two protein expressions in Zhe Lianggenao district.Three dosage groups of Chinese traditional compound medicine also increase the expression (with model group comparison, P < 0.05,0.01) of Zhe Lianggenao district NGF and BDNF as seen, and visible dose dependent.And the effect of Chinese traditional compound medicine III group is better than positive drug paroxetine group, the results are shown in Table 5.
The impact that table 5 is expressed depression rat NGF, BDNF
(n=10)
With model group comparison,
*p < 0.05,
*p < 0.01.
2.6 brief summary
This compound preparation shows the research of chronic stress depression of sex rat, and this medicine can obviously improve abnormal that depression rat behavioristics and sucrose solution take in, and strengthens the ability of learning and memory of rat model.And this compound preparation promotes that in frontal cortex and hippocampus, the expression of NGF and BDNF may be one of mechanism of its antidepressant effect.
Claims (3)
1. the compound Chinese medicinal preparation of a Cure of depression, it is characterized in that, it is prepared by the raw material of following parts by weight: Radix Bupleuri 13-17 weight portion, Fructus Aurantii Immaturus 13-17 weight portion, Rhizoma Chuanxiong 10-14 weight portion, Radix Codonopsis 8-12 weight portion, Rhizoma Atractylodis Macrocephalae 8-12 weight portion, Poria 10-14 weight portion, Cortex Magnoliae Officinalis 8-12 weight portion, Rhizoma Pinelliae 5-7 weight portion, Concha Margaritifera 15-25 weight portion, Fructus Akebiae 6-10 weight portion, Borneolum Syntheticum 1-3 weight portion, Radix Glycyrrhizae 4-8 weight portion, preparation method is: first Concha Margaritifera is pulverized, cross 60 mesh sieves, add 10 times of water yields, be decocted first after 20 minutes, again by Radix Bupleuri, Fructus Aurantii Immaturus, Rhizoma Chuanxiong, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria, Cortex Magnoliae Officinalis, Rhizoma Pinelliae, Radix Glycyrrhizae, with 12 times of medical material weight amount distilled water extraction 2-3 time, each 1-2 hour, filtered through gauze, merging filtrate, the extractum that while being evaporated to 65 DEG C, relative density is 1.15-1.20, adding ethanol makes to reach 75-85% containing the percent by volume of alcohol amount, low-temperature vacuum drying 12h, a gets dry extract, separately 70% dissolve with ethanol by 10 times of amounts by Borneolum Syntheticum, beta-schardinger dextrin-is added in 40 DEG C of distilled water and makes saturated solution, the weight of beta-schardinger dextrin-is 7 times of Borneolum Syntheticum weight, under turn 800/min stirring condition, slowly drip the above-mentioned Borneolum Syntheticum with dissolve with ethanol, stir 1h, then put cold preservation 24h in refrigerator, filter, and wash away the not beta-schardinger dextrin-of enclose of part with a small amount of distilled water, the low-temperature vacuum drying 24h b that gets dry extract, dry cream a and dry cream b are pulverized, mix, add dextrin and 70% ethanol soft material processed, rollover is granulated, gained granule is crossed 65 mesh sieves, subpackage makes granule.
2. the compound Chinese medicinal preparation of Cure of depression as claimed in claim 1, it is characterized in that, it is prepared by the raw material of following parts by weight: Radix Bupleuri 15 weight portions, Fructus Aurantii Immaturus 15 weight portions, Rhizoma Chuanxiong 12 weight portions, Radix Codonopsis 10 weight portions, the Rhizoma Atractylodis Macrocephalae 10 weight portions, Poria 12 weight portions, Cortex Magnoliae Officinalis 10 weight portions, Rhizoma Pinelliae 6 weight portions, Concha Margaritifera 20 weight portions, Fructus Akebiae 8 weight portions, Borneolum Syntheticum 2 weight portions, Radix Glycyrrhizae 5 weight portions.
As claimed in claim 1 the compound Chinese medicinal preparation of Cure of depression in the application of preparing in Cure of depression medicine.
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| CN107254803B (en) * | 2017-07-14 | 2019-05-24 | 上海维凯光电新材料有限公司 | UV coating and preparation method thereof for frangible electronic tag paper |
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| CN101658659A (en) * | 2009-05-20 | 2010-03-03 | 北京国奥健康科技研究院 | Chinese herba preparation for treating anxiety disorder |
| CN102370832A (en) * | 2011-11-17 | 2012-03-14 | 中国中医科学院中医临床基础医学研究所 | Chinese medicinal compound preparation for treating insomnia and preparation method thereof |
| CN102406917A (en) * | 2011-11-29 | 2012-04-11 | 北京阜康仁生物制药科技有限公司 | Antidepressive traditional Chinese medicine composition and preparation method thereof |
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