CN103251651A - Extracting method and application of animal ganglioside and cerebroside - Google Patents
Extracting method and application of animal ganglioside and cerebroside Download PDFInfo
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- CN103251651A CN103251651A CN2013101872296A CN201310187229A CN103251651A CN 103251651 A CN103251651 A CN 103251651A CN 2013101872296 A CN2013101872296 A CN 2013101872296A CN 201310187229 A CN201310187229 A CN 201310187229A CN 103251651 A CN103251651 A CN 103251651A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The invention relates to an extracting method and application thereof of animal ganglioside and cerebroside. The method, which is used for extracting ganglioside and cerebroside from animal spinal cord or bone marrow, comprises the following steps of: (a), extracting fat from the animal spinal cord or bone marrow by utilizing a supercritical carbon dioxide extracting method or other fat extracting methods; (b), separating the ganglioside and the cerebroside from the fat to obtain the ganglioside and the cerebroside; and (c), drying and crushing the ganglioside and the cerebroside to obtain ganglioside and cerebroside powder, wherein the ganglioside and cerebroside powder can be independently used as or mixed as a healthcare product or food, or the ganglioside and cerebroside powder can be used as materials of other healthcare products or food products to produce various healthcare products or food products including milk products, sweets, flour products, chocolate products, free-washing rice and drainage. The animal ganglioside and cerebroside extracting method disclosed by the invention can be used for sufficiently extracting the ganglioside and cerebroside from the animal spinal cord or bone marrow and applying ganglioside and cerebroside to the healthcare products or the food.
Description
Technical field
The present invention relates to a kind of animal nerve joint glycosides fat and cerebroside extracting method and its application.
Background technology
Ganglioside is the biomembrane stabilizing agent that a kind of and nerve growth, nervous system development and regeneration have substantial connection.Closely during the last ten years, in developed country, ganglioside is used also more and more widely in the treatment brain diseases.
Ganglioside accounts for about 1/1000 of brain gross weight, and molar mass average 1800, the height of its content directly have influence on growth, reparation and the regeneration of nerve fiber.Increase along with the age, long-term brain overwork and unexpected injury all may cause the minimizing of ganglioside, the lighter's giddy, memory descend, heavy then cause diseases such as dementia, cerebral palsy, in time replenishing ganglioside will be very useful for the prevention of diseases such as senile dementia, cerebral palsy, apoplexy sequela, parkinson and epilepsy.
Summary of the invention
The objective of the invention is to propose a kind of animal nerve joint glycosides fat and cerebroside extracting method and it is in health product and Application in Food.
For solving the problems of the technologies described above, the present invention adopts following technical scheme to realize:
A kind of animal nerve joint glycosides fat and cerebroside extracting method may further comprise the steps:
A. animal spinal cord or bone marrow are used supercritical carbon dioxide method for extracting or solvent extraction process, extract fat;
B. ganglioside and cerebroside are isolated from fat, obtained ganglioside and cerebroside respectively;
C. ganglioside and cerebroside is dry respectively, pulverize, make ganglioside and cerebroside powder.
Described in the step a:
During 1) with dried spinal cord or bone marrow, the dried spinal cord cleaned or bone marrow are ground into powder adopt supercritical carbon dioxide collection method or solvent extraction method, wherein oils and fats is extracted, then ganglioside and cerebroside are isolated, obtain ganglioside and cerebroside;
When adopting supercritical carbon dioxide method for extracting, at pressure 20～40MPa, under 35～60 ℃ of conditions of temperature, the time is that 2～6h extracts fat;
When adopting solvent extraction method, the solvent mixed solvent that to be chloroform mix with 2:1 with methanol is 1:1～30 adding solvents by the weight ratio of material and solvent, stripping fat;
When 2) extracting with bright spinal cord or bone marrow, earlier bright spinal cord or bone marrow are cleaned, after the drying, by above-mentioned 1) method step carry out.During with bright spinal cord or bone marrow, because bright spinal cord or bone marrow have more water very much, the extraction of influence fat is wanted earlier bright spinal cord or bone marrow drying, and then with the disposal methods of dried spinal cord or bone marrow.
Described in the step b:
In the fat that extracts, add 3～30 times petroleum ether of fat weight, stir, solid-liquid separation, solid is cerebroside, liquid is the petroleum ether solution that is dissolved with fat; Under 4 ℃ of conditions of low temperature, with adding dehydrated alcohol in the aforementioned cerebroside that obtains, residual petroleum ether is washed out, solid-liquid separation obtains the cerebroside of no solvent residue, drying, pulverizes and obtains the cerebroside powder; The aforementioned petroleum ether solution that is dissolved with fat evaporates petroleum ether through distillation, gets solid, 3～30 times the acetone that in this solid, adds solid weight, solid-liquid separation is got solid and is obtained ganglioside, will add 3～30 times dehydrated alcohol of ganglioside weight in the ganglioside that obtain, stir, residual acetone is washed out, and solid-liquid separation is got solid, obtain the ganglioside of no solvent residue, drying, pulverizing obtain ganglioside cosmetics.
Then, ganglioside and cerebroside used separately make health food or food, or it is joined make health food or food in the raw-food material.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve joint glycosides fat and cerebronic application, add ganglioside or/and the cerebroside preparation contains ganglioside or/and the cerebroside milk in milk.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve joint glycosides fat and cerebronic application, add ganglioside or/and cerebroside prepares wheaten food in flour.
A kind of animal nerve joint glycosides fat extracts with the cerebroside extracting method and obtains animal nerve joint glycosides fat and cerebronic application, in edible oil the interpolation ganglioside or/and cerebroside prepares ganglioside the cerebroside edible oil.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve joint glycosides fat and cerebronic application, add ganglioside or/and cerebroside prepares confection in syrup.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve joint glycosides fat and cerebronic application, add ganglioside or/and cerebroside prepares chocolate in cocoa butter.
A kind of animal nerve joint glycosides fat extracts with the cerebroside extracting method and obtains animal nerve joint glycosides fat and cerebronic application, in beverage stock solution the interpolation ganglioside or/and cerebroside prepares ganglioside the cerebroside beverage.
A kind of animal nerve joint glycosides fat extracts with the cerebroside extracting method and obtains animal nerve joint glycosides fat and cerebronic application, in washing-free rice the interpolation ganglioside or/and cerebroside prepares ganglioside the cerebroside washing-free rice.
Compared with prior art the invention has the beneficial effects as follows: utilize animal spinal cord and bone marrow to extract ganglioside and cerebroside fully, and be applied to health product or food.
The specific embodiment
Below by specific embodiment preparation method of the present invention and application are described in further detail:
Embodiment 1.
1) spinal cord or bone marrow animal is bright or that do wash.During with bright spinal cord or bone marrow, earlier that bright spinal cord or bone marrow is clean, dry, then, extract according to dried spinal cord or bone marrow extracting method.
During 2) with dried spinal cord or bone marrow, will clean dried spinal cord or bone marrow and be ground into powder, adopt supercritical carbon dioxide collection method or solvent extraction method, wherein oils and fats will be extracted, then ganglioside and cerebroside be isolated, obtain ganglioside and cerebroside.
When adopting supercritical carbon dioxide method for extracting, at pressure 20～40MPa, temperature is under 35～60 ℃ of conditions, and the time is that 2～6h extracts fat;
When adopting solvent extraction method, be that 1:1～30 add the mixed solvent that chloroforms and methanol mix with 2:1, stripping fat by the solid-liquid weight ratio;
The petroleum ether that adds 3～30 times in the fat that 3) will extract stirs, solid-liquid separation, and solid is cerebroside, liquid is liquid 1; Under 4 ℃ of conditions of low temperature, add dehydrated alcohol in the aforementioned cerebroside that obtains residual petroleum ether is washed out, solid-liquid separation, the gained solid is the cerebroside of no solvent residue, the cerebroside powder is made in drying, pulverizing;
Aforementioned liquid 1 evaporates petroleum ether through distillation, gets solid, the acetone that adds 3～30 times, solid-liquid separation, solid is ganglioside, with the dehydrated alcohol that adds 3～30 times in the ganglioside that obtains, stir, residual acetone is washed out solid-liquid separation, get solid, solvent evaporation residual in the solid is gone out, obtain the ganglioside of no solvent residue, ganglioside cosmetics is made in drying, pulverizing;
4) the above-mentioned ganglioside of making and cerebroside powder can use or mix use separately as health food or food or health food or raw-food material;
5) the above-mentioned ganglioside that obtains and cerebroside are joined make plurality kinds of health care product or food in the raw-food material.
As:
(1) add ganglioside or/and the processing method of cerebroside milk:
A joins ganglioside and cerebroside respectively in 30～70 ℃ the butter, perhaps ganglioside and cerebroside mix in the butter that adds 30～70 ℃, treat all to join in sweet milk or partially skimmed sweet milk or the defat sweet milk after the dissolving, make ganglioside in every 500ml sweet milk or/and cerebronic content is 0.1mg～10mg, make and contain ganglioside or/and cerebronic sweet milk;
B can will be added with ganglioside or/and the cerebroside sweet milk adds lactobacillus, presses the fermentation process fermentation of yogurt, makes yogurt;
Or/and cerebroside powder and milk powder evenly are mixed and made into health care milk powder, ganglioside is or/and cerebroside content is 0.1mg～10mg/500g to c with above-mentioned ganglioside;
(2) add ganglioside or/and the processing method of cerebronic wheaten food
Above-mentioned ganglioside or/and the cerebroside powder in the ratio of 0.1mg～10mg:500g flour, joins in the flour, through the processing processed, be can be made into wheaten food, as bread, cookies, dessert etc.;
(3) add ganglioside or/and the processing method of cerebronic edible oil
Or/and the cerebroside powder in the ratio of 0.1mg～10mg:500ml edible oil, joins in the edible oil, mix homogeneously is made edible health-care oil with above-mentioned ganglioside;
(4) add ganglioside or/and the processing method of cerebronic confection
Or/and the cerebroside powder in the ratio of 0.1mg～10mg:500g syrup, joins in sugar or the high fructose syrup, mixing is made confection by the method for processing confection afterwards with above-mentioned ganglioside;
(5) add ganglioside or/and the method for cerebronic chocolate
Above-mentioned ganglioside or/and the cerebroside powder joins mixing in the cocoa butter in the ratio of 0.1mg～10mg:500g cocoa butter, is made chocolate by the method for processing chocolate afterwards;
(6) add ganglioside or/and the processing method of cerebronic beverage
With above-mentioned ganglioside or/and the cerebroside powder joins mixing, heating for dissolving in monoglyceride or the lecithin, the monoglyceride or the lecithin that have dissolved ganglioside are joined in the beverage raw material liquid of heat, powerful stirring, homogenizing, make its emulsifying, obtain having the beverage raw material liquid of ganglioside, through the processing method of beverage, make beverage;
Ganglioside is or/and the consumption of cerebroside powder is 0.1mg～10mg:500ml beverage raw material liquid, and the consumption of monoglyceride is beverage raw material liquid 3%～5%.
(7) add ganglioside or/and the processing method of cerebronic washing-free rice
Will be in the washing-free rice that processes add above-mentioned ganglioside or/and the cerebroside powder, press ganglioside or/and cerebroside powder 0.1mg～10mg:500g washing-free rice, mixing makes the health care washing-free rice.
Claims (10)
1. an animal nerve saves glycosides fat and cerebroside extracting method, it is characterized in that may further comprise the steps:
A. animal spinal cord or bone marrow are used supercritical carbon dioxide method for extracting or solvent extraction process, extract fat;
B. ganglioside and cerebroside are isolated from fat, obtained ganglioside and cerebroside respectively;
C. ganglioside and cerebroside is dry respectively, pulverize, make ganglioside and cerebroside powder.
2. a kind of animal nerve joint glycosides fat according to claim 1 and cerebroside extracting method is characterized in that, described in the step a:
During 1) with dried spinal cord or bone marrow, the dried spinal cord cleaned or bone marrow are ground into powder adopt supercritical carbon dioxide collection method or solvent extraction method, wherein oils and fats is extracted, then ganglioside and cerebroside are isolated, obtain ganglioside and cerebroside;
When adopting supercritical carbon dioxide method for extracting, at pressure 20～40MPa, under 35～60 ℃ of conditions of temperature, the time is that 2～6h extracts fat;
When adopting solvent extraction method, the solvent mixed solvent that to be chloroform mix with 2:1 with methanol is 1:1～30 adding solvents by the weight ratio of material and solvent, stripping fat;
When 2) extracting with bright spinal cord or bone marrow, earlier bright spinal cord or bone marrow are cleaned, after the drying, by above-mentioned 1) method step carry out.
3. a kind of animal nerve joint glycosides fat according to claim 1 and cerebroside extracting method is characterized in that, described in the step b:
In the fat that extracts, add 3～30 times petroleum ether of fat weight, stir, solid-liquid separation, solid is cerebroside, liquid is the petroleum ether solution that is dissolved with fat; Under 4 ℃ of conditions of low temperature, with adding dehydrated alcohol in the aforementioned cerebroside that obtains, residual petroleum ether is washed out, solid-liquid separation obtains the cerebroside of no solvent residue, drying, pulverizes and obtains the cerebroside powder; The aforementioned petroleum ether solution that is dissolved with fat evaporates petroleum ether through distillation, gets solid, 3～30 times the acetone that in this solid, adds solid weight, solid-liquid separation is got solid and is obtained ganglioside, will add 3～30 times dehydrated alcohol of ganglioside weight in the ganglioside that obtain, stir, residual acetone is washed out, and solid-liquid separation is got solid, obtain the ganglioside of no solvent residue, drying, pulverizing obtain ganglioside cosmetics.
4. obtain animal nerve joint glycosides fat and cerebronic application according to each described a kind of animal nerve joint glycosides fat of claim 1 to 3 and the extraction of cerebroside extracting method, it is characterized in that, in milk, add ganglioside or/and the cerebroside preparation contains ganglioside or/and the cerebroside milk.
5. obtain animal nerve joint glycosides fat and cerebronic application according to each described a kind of animal nerve joint glycosides fat of claim 1 to 3 and the extraction of cerebroside extracting method, it is characterized in that, in flour, add ganglioside or/and cerebroside prepares wheaten food.
6. obtain animal nerve joint glycosides fat and cerebronic application according to each described a kind of animal nerve joint glycosides fat of claim 1 to 3 and the extraction of cerebroside extracting method, it is characterized in that, in edible oil, add ganglioside or/and cerebroside prepares ganglioside the cerebroside edible oil.
7. obtain animal nerve joint glycosides fat and cerebronic application according to each described a kind of animal nerve joint glycosides fat of claim 1 to 3 and the extraction of cerebroside extracting method, it is characterized in that, in syrup, add ganglioside or/and cerebroside prepares confection.
8. obtain animal nerve joint glycosides fat and cerebronic application according to each described a kind of animal nerve joint glycosides fat of claim 1 to 3 and the extraction of cerebroside extracting method, it is characterized in that, in cocoa butter, add ganglioside or/and cerebroside prepares chocolate.
9. obtain animal nerve joint glycosides fat and cerebronic application according to each described a kind of animal nerve joint glycosides fat of claim 1 to 3 and the extraction of cerebroside extracting method, it is characterized in that, in beverage stock solution, add ganglioside or/and cerebroside prepares ganglioside the cerebroside beverage.
10. obtain animal nerve joint glycosides fat and cerebronic application according to each described a kind of animal nerve joint glycosides fat of claim 1 to 3 and the extraction of cerebroside extracting method, it is characterized in that, in washing-free rice, add ganglioside or/and cerebroside prepares ganglioside the cerebroside washing-free rice.
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Cited By (4)
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| CN105707720A (en) * | 2016-04-14 | 2016-06-29 | 吉林省中韩动物科学研究院 | Method for preparing food products from deer skin and gangliosides |
| CN109320566A (en) * | 2018-08-14 | 2019-02-12 | 四川兴杰象药业有限公司 | A kind of preparation method for separating and purifying extracting gangliosides from pig brain marrow |
| CN109394789A (en) * | 2018-09-06 | 2019-03-01 | 四川鼎润元生物科技有限公司 | A kind of cranial nerve tissue extraction method |
| CN110917216A (en) * | 2019-12-08 | 2020-03-27 | 新疆医科大学 | Preparation method and application of bovine bone marrow lecithin |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105707720A (en) * | 2016-04-14 | 2016-06-29 | 吉林省中韩动物科学研究院 | Method for preparing food products from deer skin and gangliosides |
| CN109320566A (en) * | 2018-08-14 | 2019-02-12 | 四川兴杰象药业有限公司 | A kind of preparation method for separating and purifying extracting gangliosides from pig brain marrow |
| CN109320566B (en) * | 2018-08-14 | 2022-03-15 | 四川兴杰象药业有限公司 | Separation and purification preparation method for extracting ganglioside from pig brain marrow |
| CN109394789A (en) * | 2018-09-06 | 2019-03-01 | 四川鼎润元生物科技有限公司 | A kind of cranial nerve tissue extraction method |
| CN110917216A (en) * | 2019-12-08 | 2020-03-27 | 新疆医科大学 | Preparation method and application of bovine bone marrow lecithin |
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| CN103251651B (en) | 2015-03-18 |
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