CN103251651B - Extracting method of animal ganglioside and cerebroside - Google Patents
Extracting method of animal ganglioside and cerebroside Download PDFInfo
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- CN103251651B CN103251651B CN201310187229.6A CN201310187229A CN103251651B CN 103251651 B CN103251651 B CN 103251651B CN 201310187229 A CN201310187229 A CN 201310187229A CN 103251651 B CN103251651 B CN 103251651B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The invention relates to an extracting method and application thereof of animal ganglioside and cerebroside. The method, which is used for extracting ganglioside and cerebroside from animal spinal cord or bone marrow, comprises the following steps of: (a), extracting fat from the animal spinal cord or bone marrow by utilizing a supercritical carbon dioxide extracting method or other fat extracting methods; (b), separating the ganglioside and the cerebroside from the fat to obtain the ganglioside and the cerebroside; and (c), drying and crushing the ganglioside and the cerebroside to obtain ganglioside and cerebroside powder, wherein the ganglioside and cerebroside powder can be independently used as or mixed as a healthcare product or food, or the ganglioside and cerebroside powder can be used as materials of other healthcare products or food products to produce various healthcare products or food products including milk products, sweets, flour products, chocolate products, free-washing rice and drainage. The animal ganglioside and cerebroside extracting method disclosed by the invention can be used for sufficiently extracting the ganglioside and cerebroside from the animal spinal cord or bone marrow and applying ganglioside and cerebroside to the healthcare products or the food.
Description
Technical field
The present invention relates to a kind of animal nerve joint glycosides fat and cerebroside extracting method.
Background technology
Sphingolipids,sialo are a kind of biomembrane stabilizers having substantial connection with nerve growth, nervous system development and regeneration.Closely during the last ten years, in developed country, Sphingolipids,sialo are applied also more and more extensive in treatment encephalopathy.
Sphingolipids,sialo account for about 1/1000 of brain gross weight, molar mass average 1800, and the height of its content directly has influence on the growth of nerve fiber, reparation and regeneration.Along with the increase at age, overstrain one's nerves and unexpected injury for a long time all may cause the minimizing of Sphingolipids,sialo, the lighter is dizzy, memory declines, heavy then cause the disease such as dementia, brain paralysis, supplementing Sphingolipids,sialo in time will be very useful for the prevention of the diseases such as senile dementia, brain paralysis, apoplexy sequela, Parkinson's disease and epilepsy.
Summary of the invention
The object of the invention is to propose a kind of animal nerve joint glycosides fat and cerebroside extracting method.
For solving the problems of the technologies described above, the present invention adopts following technical scheme to realize:
A kind of animal nerve joint glycosides fat and cerebroside extracting method, comprise the following steps:
A. animal spinal cord or marrow are used supercritical carbon dioxide method for extracting or solvent extraction process, extract fat;
B. Sphingolipids,sialo and cerebroside are isolated from fat, obtain Sphingolipids,sialo and cerebroside respectively;
C. Sphingolipids,sialo and cerebroside be dry, pulverize respectively, make Sphingolipids,sialo and cerebroside powder.
Described in step a:
1) time with dry spinal cord or marrow, the dry spinal cord cleaned or marrow powder are broken into powder and adopt supercritical co extraction method or solvent-extraction process, grease is wherein extracted, then Sphingolipids,sialo and cerebroside is isolated, obtain Sphingolipids,sialo and cerebroside;
When adopting supercritical carbon dioxide method for extracting, at pressure 20 ~ 40MPa, under temperature 35 ~ 60 DEG C of conditions, the time is that 2 ~ 6h extracts fat;
When adopting solvent-extraction process, solvent is the mixed solvent that chloroform mixes with 2:1 with methyl alcohol, is that 1:1 ~ 30 add solvent by the weight ratio of material and solvent, stripping fat;
2), when extracting with fresh spinal cord or marrow, first fresh spinal cord or marrow are cleaned, after drying, by above-mentioned 1) method steps carry out.During with fresh spinal cord or marrow, because fresh spinal cord or marrow have more moisture very much, the extraction of impact fat, first that fresh spinal cord or marrow is dry, and then by the disposal methods of dry spinal cord or marrow.
Described in step b:
In the fat extracted, add the sherwood oil of 3 ~ 30 times of fat weight, stir, solid-liquid separation, solid is cerebroside, and liquid is the petroleum ether solution being dissolved with fat; Under low temperature 4 DEG C of conditions, add dehydrated alcohol, washed out by residual sherwood oil, solid-liquid separation, obtain the cerebroside of no solvent residue by the aforementioned cerebroside obtained, drying, pulverizing obtain cerebroside powder; The aforementioned petroleum ether solution being dissolved with fat passes through distillation, is evaporated by sherwood oil, gets solid, the acetone of 3 ~ 30 times of solid weight is added in this solid, solid-liquid separation, gets solid and obtains Sphingolipids,sialo, will add the dehydrated alcohol of 3 ~ 30 times of Sphingolipids,sialo weight in the Sphingolipids,sialo obtained, stir, residual acetone is washed out, solid-liquid separation, get solid, obtain the Sphingolipids,sialo of no solvent residue, drying, pulverizing obtain ganglioside cosmetics.
Then, Sphingolipids,sialo and cerebroside are used alone and make protective foods or food, or joined in food raw material and make protective foods or food.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve and save glycosides fat and cerebronic application, add Sphingolipids,sialo or/and prepared by cerebroside contain Sphingolipids,sialo or/and cerebroside dairy products in dairy products.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve and save glycosides fat and cerebronic application, add Sphingolipids,sialo or/and wheaten food prepared by cerebroside in flour.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve and save glycosides fat and cerebronic application, add Sphingolipids,sialo or/and Sphingolipids,sialo prepared by cerebroside or/and cerebroside edible oil in edible oil.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve and save glycosides fat and cerebronic application, add Sphingolipids,sialo or/and candy prepared by cerebroside in syrup.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve and save glycosides fat and cerebronic application, add Sphingolipids,sialo or/and chocolate prepared by cerebroside in theobroma oil.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve and save glycosides fat and cerebronic application, add Sphingolipids,sialo or/and Sphingolipids,sialo prepared by cerebroside or/and cerebroside beverage in beverage stoste.
A kind of animal nerve joint glycosides fat and cerebroside extracting method extract and obtain animal nerve and save glycosides fat and cerebronic application, add Sphingolipids,sialo or/and Sphingolipids,sialo prepared by cerebroside or/and cerebroside washing-free rice in washing-free rice.
Compared with prior art the invention has the beneficial effects as follows: utilize animal spinal cord and marrow to extract Sphingolipids,sialo and cerebroside fully, and be applied to healthcare products or food.
Embodiment
Below by specific embodiment, preparation method of the present invention and application are described in further detail:
Embodiment 1.
1) spinal cord fresh or dry for animal or marrow are washed.During with fresh spinal cord or marrow, first fresh spinal cord or marrow are cleaned, dry, then, extract according to dry spinal cord or marrow extracting method.
2) time with dry spinal cord or marrow, clean dry spinal cord or marrow powder are broken into powder, adopt supercritical co extraction method or solvent-extraction process, grease is wherein extracted, then Sphingolipids,sialo and cerebroside are isolated, obtain Sphingolipids,sialo and cerebroside.
When adopting supercritical carbon dioxide method for extracting, at pressure 20 ~ 40MPa, temperature is under 35 ~ 60 DEG C of conditions, and the time is that 2 ~ 6h extracts fat;
When adopting solvent-extraction process, be the mixed solvent that 1:1 ~ 30 add chloroform and mix with 2:1 with methyl alcohol by solid-liquid weight ratio, stripping is fatty;
3) by adding the sherwood oil of 3 ~ 30 times in the fat extracted, stir, solid-liquid separation, solid is cerebroside, and liquid is liquid 1; Washed out adding dehydrated alcohol in the aforementioned cerebroside obtained by residual sherwood oil under low temperature 4 DEG C of conditions, solid-liquid separation, gained solid is the cerebroside of no solvent residue, and drying, pulverizing make cerebroside powder;
Sherwood oil, through distillation, evaporates, gets solid by aforementioned liquid 1, add the acetone of 3 ~ 30 times, solid-liquid separation, solid is Sphingolipids,sialo, the dehydrated alcohol of 3 ~ 30 times will be added in the Sphingolipids,sialo obtained, stir, residual acetone is washed out, solid-liquid separation, get solid, evaporated by the solvent remained in solid, obtain the Sphingolipids,sialo of no solvent residue, drying, pulverizing make ganglioside cosmetics;
4) the above-mentioned Sphingolipids,sialo made and cerebroside powder, can be used alone or used in combination as protective foods or food or protective foods or food raw material;
5) Sphingolipids,sialo obtained above and cerebroside are joined in food raw material make plurality kinds of health care product or food.
As:
(1) Sphingolipids,sialo are added or/and the working method of cerebroside dairy products:
Sphingolipids,sialo and cerebroside join in the cream of 30 ~ 70 DEG C by a respectively, or Sphingolipids,sialo and cerebroside mixing add in the cream of 30 ~ 70 DEG C, join in sweet milk or partially skimmed sweet milk or degreasing sweet milk after all dissolving, make Sphingolipids,sialo in every 500ml sweet milk or/and cerebronic content is 0.1mg ~ 10mg, make containing Sphingolipids,sialo or/and cerebronic sweet milk;
B can will be added with Sphingolipids,sialo or/and cerebroside sweet milk adds milk-acid bacteria, ferments, make yogurt by the fermentation process of yogurt;
C is by above-mentioned Sphingolipids,sialo or/and cerebroside powder and milk powder Homogeneous phase mixing make health care milk powder, and Sphingolipids,sialo are or/and cerebroside content is 0.1mg ~ 10mg/500g;
(2) Sphingolipids,sialo are added or/and the working method of cerebronic wheaten food
By above-mentioned Sphingolipids,sialo or/and cerebroside powder is in the ratio of 0.1mg ~ 10mg:500g flour, join in flour, through processing shortening, can be made into wheaten food, as bread, biscuit, dessert etc.;
(3) Sphingolipids,sialo are added or/and the working method of cerebronic edible oil
By above-mentioned Sphingolipids,sialo or/and cerebroside powder is in the ratio of 0.1mg ~ 10mg:500ml edible oil, join in edible oil, mix, make edible health-care oil;
(4) Sphingolipids,sialo are added or/and the working method of cerebronic candy
By above-mentioned Sphingolipids,sialo or/and cerebroside powder is in the ratio of 0.1mg ~ 10mg:500g syrup, join in sugar or high fructose syrup, mixing, make candy by the method for processing candy afterwards;
(5) Sphingolipids,sialo are added or/and the method for cerebronic chocolate
By above-mentioned Sphingolipids,sialo or/and cerebroside powder joins in theobroma oil in the ratio of 0.1mg ~ 10mg:500g theobroma oil mixes, make chocolate by the method for processing chocolate afterwards;
(6) Sphingolipids,sialo are added or/and the working method of cerebronic beverage
By above-mentioned Sphingolipids,sialo or/and cerebroside powder joins mixing, heating for dissolving in Tegin 55G or Yelkin TTS, the Tegin 55G dissolving Sphingolipids,sialo or Yelkin TTS are joined in the beverage materials liquid of heat, strong stirring, homogeneous, make its emulsification, obtain the beverage materials liquid with Sphingolipids,sialo, through the working method of beverage, make beverage;
Sphingolipids,sialo are or/and the consumption of cerebroside powder is 0.1mg ~ 10mg:500ml beverage materials liquid, and the consumption of Tegin 55G is beverage materials liquid 3% ~ 5%.
(7) Sphingolipids,sialo are added or/and the working method of cerebronic washing-free rice
To above-mentioned Sphingolipids,sialo be added in the washing-free rice processed or/and cerebroside powder, by Sphingolipids,sialo or/and cerebroside powder 0.1mg ~ 10mg:500g washing-free rice, mixing, obtained health care washing-free rice.
Claims (1)
1. animal nerve joint glycosides fat and a cerebroside extracting method, is characterized in that comprising the following steps:
A. animal spinal cord or marrow are used supercritical carbon dioxide method for extracting or solvent extraction process, extract fat: in step a:
1), time with dry spinal cord or marrow, the dry spinal cord cleaned or marrow powder are broken into powder and adopt supercritical carbon dioxide method for extracting or solvent-extraction process, grease is wherein extracted;
When adopting supercritical carbon dioxide method for extracting, at pressure 20 ~ 40MPa, under temperature 35 ~ 60 DEG C of conditions, the time is that 2 ~ 6h extracts fat;
When adopting solvent-extraction process, solvent is the mixed solvent that chloroform mixes with 2:1 with methyl alcohol, is that 1:1 ~ 30 add solvent by the weight ratio of material and solvent, stripping fat;
2), when extracting with fresh spinal cord or marrow, first fresh spinal cord or marrow are cleaned, after drying, by above-mentioned 1) method steps carry out;
B. Sphingolipids,sialo and cerebroside are isolated from fat, obtain Sphingolipids,sialo and cerebroside respectively;
In the fat extracted, add the sherwood oil of 3 ~ 30 times of fat weight, stir, solid-liquid separation, solid is cerebroside, and liquid is the petroleum ether solution being dissolved with fat; Under low temperature 4 DEG C of conditions, add dehydrated alcohol by the aforementioned cerebroside obtained, residual sherwood oil is washed out, solid-liquid separation, obtain the cerebroside of no solvent residue;
The aforementioned petroleum ether solution being dissolved with fat passes through distillation, sherwood oil is evaporated, gets solid, in this solid, add the acetone of 3 ~ 30 times of solid weight, solid-liquid separation, get solid and obtain Sphingolipids,sialo, will in the Sphingolipids,sialo obtained, add the dehydrated alcohol of 3 ~ 30 times of Sphingolipids,sialo weight, and stir, residual acetone is washed out, solid-liquid separation, gets solid, obtains the Sphingolipids,sialo of no solvent residue;
C. Sphingolipids,sialo and cerebroside be dry, pulverize respectively, make ganglioside cosmetics and cerebroside powder.
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Families Citing this family (4)
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| CN105707720A (en) * | 2016-04-14 | 2016-06-29 | 吉林省中韩动物科学研究院 | Method for preparing food products from deer skin and gangliosides |
| CN109320566B (en) * | 2018-08-14 | 2022-03-15 | 四川兴杰象药业有限公司 | Separation and purification preparation method for extracting ganglioside from pig brain marrow |
| CN109394789A (en) * | 2018-09-06 | 2019-03-01 | 四川鼎润元生物科技有限公司 | A kind of cranial nerve tissue extraction method |
| CN110917216A (en) * | 2019-12-08 | 2020-03-27 | 新疆医科大学 | Preparation method and application of bovine bone marrow lecithin |
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Effective date of registration: 20190830 Address after: 130600 No. 1 Liu highway, Shuangyang Economic Development Zone, Changchun, Jilin Patentee after: JILIN DONGAO SUPREME TECHNOLOGY DEVELOPMENT CO., LTD. Address before: 130600 Donghua Street, Shuangyang District, Changchun City, Jilin Province, 1699 Patentee before: Jilin China and Korea Animal Science and Technology Research Institute |
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