CN103245743A - Rapid detection method for expired gas through on-line enrichment gas chromatography - Google Patents
Rapid detection method for expired gas through on-line enrichment gas chromatography Download PDFInfo
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- CN103245743A CN103245743A CN2013101692471A CN201310169247A CN103245743A CN 103245743 A CN103245743 A CN 103245743A CN 2013101692471 A CN2013101692471 A CN 2013101692471A CN 201310169247 A CN201310169247 A CN 201310169247A CN 103245743 A CN103245743 A CN 103245743A
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Abstract
The invention discloses a rapid detection method for expired gas through an on-line enrichment gas chromatography. The method mainly comprises the steps that: a collector is selected by using the expired gas to collect alveolar gas in the expired gas; VOCs (Volatile Organic Compounds) in the alveolar gas are adsorbed by a preconcentrator; the preconcentrator is heated to enable the VOCs enriched on an adsorbent to be desorbed; the preconcentrator is blown back by carrier gas to enable the VOCs subjected to the heat desorption to enter a chromatographic separation column along with the carrier gas for separation; and component gases of the VOCs after the separation enter a microplasma detector along with the carrier gas. After component gas molecules of the VOCs enter a discharge chamber of the microplasma detector, the component gas molecules are excited by the existing high-energy metastable-state particles, then are jumped back to ground states, and send characteristic optical signals; the characteristic optical signals sent by the jumping of the component molecules of the VOCs are focused and coupled to an optical fiber by a lens; the characteristic optical signals are transmitted by the optical fiber to a spectrograph for detection; a computer processes and stores data; and the rapid detection on the VOCs in the expired gas is realized. With the adoption of the method, components of the expired gas can be monitored in real time and on line.
Description
Technical field
The present invention relates to the detection technique of trace volatile organic compounds in the human body expiratory air, particularly relate to the method for trace volatile organic compounds in the expiratory air being carried out fast detecting with the on-line preconcentration portable gas chromatography.
Background technology
In recent years, China is that the major disease of representative presents tangible fast rise trend with the cardiovascular and cerebrovascular disease.Current " bottleneck " to these major disease controls is: for disease early detection, early diagnosis understanding inadequately, the effective measures that lack early diagnosis, early warning and monitoring, to such an extent as to increased the misery of patient and family, influenced cure rate, also brought enormous economic loss.National Program for Medium-to Long-term Scientific and Technological Development (2006-2020) proposes: with Centre for Disease Control's reach, study the gordian technique of prevention and early diagnosis, significantly improve diagnosis and the prevention and control capability of major disease.Therefore, the new method of research major disease early diagnosis and examination has great strategic importance.
In the human body generation of a lot of diseases all with expiratory air in trace volatile organic compounds (volatile organic compounds, variation VOCs) is relevant.Before occurring at disease symptoms, the oxidative stress in the human body will be different, and corresponding VOCs will appear in the expiratory air.The VOCs that has reported comprises isoprene, acetone, is a series of alkane of representative etc. with the pentane.These VOCs extensively are present in the different individual expiratory airs, and reflect certain Human Physiology and pathologic condition.Because the metabolic product of human body can be transported to lung via blood, entering alveolar by the gas exchange appears in the expiratory air, in recent years, expiratory air detects as a kind of understanding human physiological metabolism, and particularly the new method of major disease early diagnosis is greatly paid attention to.
Compare with the blood testing of routine, the largest benefit that expiratory air detects is exactly to the human zero damage, and the expiratory air that only needs to gather patient just can detect patient comfort, no pain, hommization more.And the sampling of expiratory air is simple and convenient, but repeated acquisition is convenient to the real-time monitoring of the state of an illness.And conventional blood collection usually needs to gather venous blood or finger tip blood sample, and times of collection is too much, can bring great misery to patient, particularly old man, pregnant woman and child, the also real-time monitoring of the inconvenience state of an illness.
The human body expiratory air mainly is made up of nitrogen, carbon dioxide, oxygen, water vapor, inert gas and trace volatile organic compounds (VOCs).VOCs reaches the hundreds of kind in the present detected human body, and its volumetric concentration scope between the pptv, mainly contains acetone, isoprene, pentane etc. at ppmv.Interindividual widely different, but along with the increasing of individual amount, total VOCs quantity is logarithm trend and reduces between Different Individual, and on average the VOCs quantity in each individual expiratory air has about 200 kinds.These compounds comprise alkane, alkene, group compounds of aldehydes and ketones, sulfocompound and nitrogen-containing compound etc.They all are the products of body metabolism process, and are closely related with the individual health situation.Therefore a lot of disease researches begin to pay close attention to the VOCs in the expiratory air.M.2006) etc. (Phillips discovers to have at least the content of 9 kinds of VOCs generally to be higher than normal population in lung cancer crowd's the expiratory air to Phillips, mainly is alkane and derivant thereof, the derivant of benzene.(Sandra V.V.2008) discovers Sandra etc., and in the expiratory air of hepatocarcinoma patient, the content of dimethyl sulfide, acetone, 2-butanone, 2 pentanone is apparently higher than the normal person.Phillips etc. (Phillips, M.2006) discover in the breast cancer patient expiratory air 5 kinds of VOCs are arranged content apparently higher than normal population, be respectively isopropyl alcohol, phenylchinoline ketone, methyl cyclohexanone, enanthaldehyde and isopropyl myristate.
In the existing detection expiratory air there be much the method for VOCs.Wherein, depending on gas chromatography-hydrogen flame detection method (GC-FID) and the gas chromatography-mass spectrum method (GC-MS) that vapor-phase chromatography sets up is the main method of trace VOCs in the detection expiratory air.The pick-up unit of these detection methods generally all comprises these several parts of carrier gas system, sampling system, chromatographic fractionation system, detecting device and data handling system, and wherein chromatography column need have the ability of temperature programme.Usual earlier use GC-FID detects the VOCs in the expiratory air, has superiority though GC-FID separates and quantitatively detect potpourri, and it can not differentiate the compound of the unknown in the expiratory air.In view of the deficiency of the qualitative ability of GC-FID, the researchist is attached to mass spectrum detection in the gas chromatography, obtains gas chromatography-mass spectrum method (GC-MS).The GC-MS method has become the main method that VOCs separates detection in the expiratory air at present.But implement the apparatus structure complexity of this method, bulky, need provide cyclinder gas as carrier gas and combustion gas, chromatogram column temperature is regulated the casing that depends on large volume, be not easy to carry, and analysis time is long, significant discomfort is used in the occasion of, fast detecting on-the-spot instant at needs.
In view of the existing deficiency that detects VOCs method existence in the expiratory air, practice needs the VOCs detection method in the new expiratory air of exploitation, to realize the trace VOCs in the real-time online detection expiratory air.
Summary of the invention
Present situation at VOCs Fast Detection Technique in the existing expiratory air, purpose of the present invention aims to provide VOCs detection method in a kind of new expiratory air, with overcome VOCs detection method device for carrying out said exists in the existing expiratory air apparatus structure complexity, bulky, expensive, complicated operation, analysis time long, can not realize the deficiency that quick real-time online detects.
Expiratory air on-line preconcentration gas chromatography method for quick provided by the invention mainly may further comprise the steps:
(1) expiratory air is blown into breath and selects gatherer, select to collect the alveolar gas in the expiratory air;
(2) alveolar gas of selecting to collect is sent into preconcentrator, utilize the VOCs in the adsorbents adsorb alveolar air in the preconcentrator, waste gas is discharged;
(3) the heating preconcentrator makes the VOCs desorption that is enriched on the adsorbent;
(4) microplasma work gas is fed the microplasma detecting device, make microplasma work gas form microplasma under high voltage in arc chamber, this microplasma is made up of metastable atom, ion, electronics, and integral body is electric neutrality;
(5) sweep adsorbent in the preconcentrator with the carrier gas blowback, the VOCs that pyrolysis is analysed get off enters chromatography column with carrier gas, carries out component and separates;
(6) separate the VOCs component gas that obtains through chromatography column and enter the microplasma detecting device with carrier gas, above-mentioned VOCs component gas molecule enters behind the microplasma detecting device arc chamber by existing high-octane metastable particle (metastable atom, ion, electronics) wherein and excites, and transition is subsequently returned ground state and sent the characteristic light signal;
(7) utilize lens that the characteristic light signal focus that each component molecular transition of VOCs sends is coupled to optical fiber, detected to spectrometer by Optical Fiber Transmission, and handle and the storage data by computer, realize the fast detecting to VOCs in the expiratory air.
In above-mentioned expiratory air method for quick of the present invention, described alveolar gas is the expiratory air behind the discharge dead volume pneumatosis in the expiratory air; Be the gas of last 350ml in the expiratory air further.
In above-mentioned expiratory air method for quick of the present invention, described alveolar gas can be via the fixing VOCs in the alveolar air of an adsorption section absorption that is made of interlayer and adsorbent in preconcentrator, also can adsorb VOCs in the fixing alveolar air via plural adsorption section; The preferential employing via the fixing VOCs in the alveolar air of two or more adsorption sections absorption, the adsorbent type that constitutes each adsorption section can be identical adsorbent, also can be the dissimilar adsorbent of different adsorption strengths.The dissimilar adsorbent of preferential employing.Adsorbent specifically can be selected acticarbon, vermiculite power, carbonaceous molecular sieve 1000, Graphon etc. for use, and they have cheap and easy to get and characteristics advantages of good adsorption effect.Constituting the interlayer of adsorption section, is for dissimilar adsorbents being separated and be limited in the preconcentrator, be generally the porous heat-insulating material, preferentially adopting glass wool.
In above-mentioned expiratory air method for quick of the present invention, the VOCs in the alveolar gas is general in preconcentrator to adopt the mode by physisorption to be fixed on the adsorbent surface.Gas as VOCs carrier gas and work gas is generally inert gas, specifically can select argon gas, helium or nitrogen for use, preferentially selects argon gas for use.The miniature compression gas tank gas of the preferential employing of gas source as VOCs carrier gas and work gas.
In above-mentioned expiratory air method for quick of the present invention, VOCs enters chromatography column with carrier gas and carries out the component separation, and its lock out operation carries out at normal temperatures, carries out under 20～40 ℃ usually.
In above-mentioned expiratory air method for quick of the present invention, separate each component gas of VOCs that obtains through chromatography column and preferably enter the microplasma detecting device successively in the mode of pulse, be VOCs component and current-carrying gas alternate enter the microplasma detecting device successively, each component gas of VOCs is excited by existing high-octane metastable particle (metastable atom, ion, electronics) wherein in microplasma detecting device arc chamber, and transition is subsequently returned ground state and sent the characteristic light signal.
Implement the on-line preconcentration gas chromatography device for fast detecting of the above-mentioned expiratory air method for quick of the present invention, its structure mainly comprises the expiratory air collector, gatherer is selected in expiratory air, preconcentrator, the expiratory air discharge pump, the inertia source of the gas, chromatography column, the microplasma detecting device, and lens, spectrometer and computer, the expiratory air discharge pump is arranged on the pipeline of the front of preconcentrator or back, the preconcentrator front-end interface is connected with the air intake opening of chromatography column with selecting the gatherer outlet respectively by operation valve, be connected with work gas import on the minisize plasma detector with the preconcentrator back end interface respectively by pipeline and operation valve as the source of the gas of carrier gas and work gas, the preconcentrator back end interface is provided with outlet port with the pipeline that the inertia source of the gas is connected, the post tail of chromatography column inserts the gas feed of microplasma detecting device testing sample, the corresponding setting of arc chamber window of lens and microplasma detecting device, by lens the characteristic light signal focus that each component molecular transition of VOCs sends is coupled to optical fiber, detect processing by Optical Fiber Transmission to spectrometer again, and then be transferred to computer, handled and the storage data by computer, realize the fast detecting to VOCs in the expiratory air.
In above-mentioned device for fast detecting, the physical dimension of described microplasma detecting device is not more than long * 500 μ m of 600 μ m wide * 500 μ m height, cumulative volume is not more than 150nL.Be made of with the sample introduction joint that is connected with arc chamber arc chamber, be provided with 2 sparking electrodes that are connected with direct supply in the arc chamber, the sample introduction joint design has gas sampling interface to be measured and work gas interface.
In above-mentioned device for fast detecting, described expiratory air gas selector switch is made of syringe, T-valve and collection chamber, two interfaces of T-valve are connected with collection chamber with syringe respectively, another interface is vacant, by the operation T-valve, make dead gas in the breath by vacant interface emptying, make that alveolar gas enters into preconcentrator in the breath.
In above-mentioned device for fast detecting, described preconcentrator by shell thick between the detail of two ends, be positioned at shell adsorption section, be wrapped in the outer resistive heater of shell and be wrapped in the outer thermofin of resistive heater and constitute.Described adsorption section is made of adsorbent and interlayer that adsorbent is limited in the shell.Described resistive heater is connected with direct supply.The material of described shell can be quartz glass, stainless steel or metal alloy.
In above-mentioned device for fast detecting, be the flow velocity of the alveolar gas selected by discharge pump control gas selector switch, make alveolar gas can slowly flow through preconcentrator equably.Described expiratory air discharge pump can be the pressure discharge pump, also can be vacuum pump, and when it was the pressure discharge pump, it was arranged on the front of preconcentrator, and when it was vacuum pump, it was arranged on the back of preconcentrator.The preferential vacuum pump that adopts.
In above-mentioned device for fast detecting, can further consider at the pipeline that connects chromatography column air intake opening and preconcentrator front-end interface mass flow controller is set, flow into the gas flow of chromatographic column with control; At the pipeline that connects microplasma sensors work gas import and inertia source of the gas mass flow controller is set, flows into the working air current amount of microplasma detecting device with control.
Expiratory air on-line preconcentration gas chromatography method for quick provided by the invention, it is the alveolar gas of selecting earlier to collect in the expiratory air, again the VOCs in the alveolar gas is enriched on the adsorbent, mode by the pulse sample introduction is blown into chromatography column fast with the VOCs under the thermal desorption at last, carrying out component at normal temperatures separates, component gas after the separation directly enters the microplasma detecting device from chromatography column, the component gas molecule in microplasma detecting device arc chamber by existing high-octane metastable particle (metastable atom wherein, ion, electronics) excites, transition is subsequently returned ground state and is sent the characteristic light signal, the certain optical signals of sending is focused on by lens and is coupled to optical fiber and is transferred to spectrometer again and detects processing, and demonstrate signal processing results and store data by computer, thereby realize fast detecting to VOCs in the expiratory air.The present invention is attached to the breath selector switch in the pick-up unit innovatively, selects alveolar gas by a simple detachable syringe, has avoided by measuring CO in the expiratory air
2Concentration is collected the complicated approach of alveolar gas.The present invention has adopted the method for on-line preconcentration pulse sample introduction to expiratory air, can realize the quick sampling sample introduction.The present invention uses the normal temperature chromatographic column to separate, and has avoided the chromatographic column intensification, has simplified device, has reduced energy consumption.In addition, the present invention utilizes the microplasma device as detecting device, simplified detecting device structure, reduced the cost of structure detecting device, reduced energy consumption.
Because adopted above a series of technical measures, the present invention has following outstanding feature:
(1) separation of VOCs component is with a kind of inert gas with the work gas of carrier gas and microplasma detecting device, and a gas is dual-purpose, has reduced the gas tank that need carry.
(2) adopt specially designed expiratory air to select gatherer, can collect the alveolar gas in the expiratory air quickly and easily, avoided the pollution dilution of dead volume gas to expiratory air, also avoided by measuring CO in the expiratory air
2Concentration is collected the complex operations that alveolar gas is brought, and method is simple, is conducive to the stable expiratory air detection that repeatedly realizes, therefore can obtain reliable and stable testing result.
(3) combination is swept in breath selection, the absorption of alveolar air pre-concentration, VOCs carrier gas blowback, realized that VOCs gas fast-pulse sample introduction is in chromatography column.
(4) use normal temperature chromatographic column is separated the VOCs in the expiratory air, has avoided the chromatographic column intensification, has reduced energy consumption, has simplified the chromatographic column system.
(5) use the microplasma detecting device to detect VOCs in the expiratory air, energy consumption is little, and has simplified detecting device, has reduced the detecting device cost.
(6) device volume is little, can carry, and has realized that real-time online detects the VOCs component in the expiratory air.
Description of drawings
Fig. 1 is the one-piece construction synoptic diagram of expiratory air on-line preconcentration gas chromatography method for quick device for carrying out said of the present invention.
Fig. 2 is the enlarged drawing of the local A of Fig. 1, and wherein Fig. 2-a is the axial arrangement synoptic diagram of preconcentrator 3-1 among Fig. 1; Fig. 2-b is preconcentrator 3-1 section structure synoptic diagram.
Fig. 3 is the enlarged drawing of the local B of Fig. 1, and wherein Fig. 3-a is microplasma detecting device 5-3 structural representation; Fig. 3-b is the connection diagram of minisize plasma detector and direct supply.
Fig. 4 is the structural representation of expiratory air gas selector switch 2 among Fig. 1.
Each shown by reference numeral of above-mentioned accompanying drawing sign object is respectively: 1. expiratory air blow gun; 1-1. air blowing pipeline.2. breath is selected gatherer; 2-1. syringe; 2-2. T-valve; 2-3. collection chamber; 2-4. emptying pipeline.3-1. preconcentrator; 3-1-1. quartz glass tube; 3-1-2. glass wool interlayer; 3-1-3. adsorbent; 3-1-4. adsorbent; 3-1-5. adsorbent; 3-1-6. heat insulation layer; The 3-1-7 resistive heater; 3-2. direct supply.4. source of the gas; 4-1. gas source pipe; 4-1-1. purging carrier gas channel; 4-1-2. work feed channel; 4-1-1-1. alveolar gas conveyance conduit; 4-1-1-2.VOCs carrier gas conveyance conduit.5-1. mass flow controller; 5-2. chromatography column; 5-2-1. chromatography column post tail; 5-3. microplasma detecting device: 5-3-1. arc chamber; 5-3-2. sparking electrode; 5-3-3. direct supply; 5-3-4. work gas entrance; 5-3-5. electric power connection line.5-4. mass flow controller; 6-1. lens; 6-2. spectrometer; 6-3. computer; 6-4. optical fiber; 7-1. T-valve, 7-2. T-valve; 7-3. T-valve; 8. needle-valve; 9. vacuum pump.
Embodiment
Be further described specifically below by the present invention of embodiment.Be necessary to point out at this, the following examples just are used for setting forth better principle of work of the present invention and practical application thereof, so that the technician in other field is used for the various facilities in its field with the present invention, and improve according to the imagination of various special-purposes.Although the present invention discloses its first-selected embodiment by literal; but can understand wherein the property optimized and alterability by reading these technology explanatory notes; and improve not departing from scope and spirit of the present invention, but such improvement should still belong to the protection domain of claim of the present invention.
Embodiment
The expiratory air on-line preconcentration gas chromatography method for quick of present embodiment is as follows:
(1) by the expiratory air blow gun 1 as the expiratory air collector, expiratory air is blown into breath selects gatherer 2, selection collection breath is discharged the alveolar gas of the last 350ml behind the dead gas;
(2) vacuum pump 9 by being arranged on the preconcentrator back is pumped into preconcentrator 3-1 with the alveolar gas of the last 350ml that selects to collect, VOCs in 3 adsorption sections absorption alveolar gas that constituted respectively by the adsorbent of different adsorption strengths and interlayer, VOCs in the alveolar gas is fixed on the adsorbent surface, and the VOCs in the alveolar air is adsorbed back remaining gas part and is discharged from by vacuum pump; The adsorbent of three adsorption sections is respectively acticarbon, vermiculite power and carbonaceous molecular sieve 1000, and the material of interlayer is glass wool;
(3) preconcentrator is heated, make the VOCs desorption that is enriched on the adsorbent;
(4) microplasma work gas Ar gas is fed the microplasma detecting device, make Ar gas form microplasma under high voltage in arc chamber, this microplasma is made up of metastable atom, ion, electronics, and integral body is electric neutrality;
(5) with argon gas as VOCs current-carrying gas, adsorbent in the preconcentrator is swept in blowback, the VOCs that pyrolysis is analysed get off enters chromatography column 5-2 with current-carrying gas Ar gas through mass flowmeter 5-1, carries out component and separate in about 25 ℃;
(6) separate each component gas of VOCs obtain through chromatography column and enter the microplasma detecting device with argon gas successively with the form of pulse, above-mentioned VOCs component gas molecule enters behind the microplasma detecting device arc chamber by existing high-octane metastable particle (metastable atom, ion, electronics) wherein and excites, and transition is subsequently returned ground state and sent the characteristic light signal;
(7) utilize lens 6-1 that the characteristic light signal focus that each component molecular transition of VOCs sends is coupled to optical fiber, detect processing by Optical Fiber Transmission to spectrometer 6-2, and handle and the storage data by computer 6-3, realize the fast detecting to VOCs in the expiratory air.
The device for carrying out said of present embodiment expiratory air on-line preconcentration gas chromatography method for quick, its structure to shown in the accompanying drawing 4, comprises mainly that blow gun 1, the breath as the expiratory air collector selected gatherer 2, preconcentrator 3-1, vacuum pump 9, argon gas compression gas tank 4, chromatography column 5-2, microplasma detecting device 5-3, lens 6-1, spectrometer 6-2 and computer 6-3 as accompanying drawing 1.Described expiratory air blow gun 1 is for surveying the used blow gun of vital capacity, and described breath selects gatherer 2 to be made of detachable teflon syringe 2-1 processed, T-valve 2-2, collection chamber 2-3, air pipe line 2-4.Described breath selects gatherer 2, preconcentrator 3-1, vacuum pump 9 to link together in order by incoming call gas pipeline 4-1-1-1, argon gas current-carrying feed channel 4-1-1, swivel tee valve 7-1 and swivel tee valve 7-2; Described argon gas compression gas tank 4, preconcentrator 3-1, chromatography column 5-2, minisize plasma detector 5-3 are swept current-carrying feed channel 4-1-1, swivel tee valve 7-1, mass flowmeter 5-1, mass flowmeter 5-4, argon gas work feed channel 4-1-2, VOCs current-carrying letter shoot road 4-1-1-2 by argon gas compression feed channel 4-1, T-valve 7-3, needle-valve 8, swivel tee valve 7-2, argon gas blowback and are linked together in order; The described lens 6-1 of the optical signals that described microplasma detecting device 5-3 sends focuses on, and described lens 6-1 is connected with spectrometer 6-2 by optical fiber 6-4, and spectrometer 6-2 is connected with computer 6-3 again.When gathering expiratory air, breath takes off pull bar after selecting the syringe 2-1 pull bar of gatherer 2 to move the end to, afterwards with tidal air blow gun 1, syringe 2-1 and emptying are taken over 2-4 and are communicated with by air blowing pipeline 1-1 and T-valve 2-2, by the control T-valve expiratory air previous section gas is discharged in the air, 350ml alveolar gas last in the expiratory air is stayed in the syringe, swivel tee valve 2-2 afterwards, make syringe 2-1, collection chamber 2-3 is connected together, take off syringe 2-1 and go up the air blowing pipeline 1-1 that connects, to screw on the supporting pull bar of syringe 2-1, the alveolar gas among the syringe 2-1 will be squeezed among the collection chamber 2-3.When concentrating in the tidal air VOCs, swivel tee valve 2-2 closes 2-2 to the path of collection chamber 2-3, turn T-valve 7-1 and T-valve 7-2, collection chamber 2-3, preconcentrator 3-1, vacuum pump 9 are communicated with, with vacuum pump 9 alveolar gas among the collection chamber 2-3 is entered into preconcentrator 3-1, by the VOCs in the adsorbents adsorb alveolar gas in the preconcentrator shell, all the other waste gas are discharged from vacuum pump 9.After finishing, absorption closes vacuum pump 9, turn swivel tee valve 7-1 and 7-2 only communicate preconcentrator 3-1 with the carrier gas channel 4-1-1-2 at chromatography column place, open direct supply 3-2, heating preconcentrator 3-1, the VOCs of adsorbent surface absorption is analysed in pyrolysis.Open argon gas compression gas tank 4 subsequently, enter microplasma detecting device 5-3 as the argon gas of microplasma work gas through work feed channel 4-1-2, open direct supply 5-3-3 again, form the microplasma chamber at arc chamber 5-3-1.When detecting in the tidal air VOCs, determine that the VOCs desorption of absorption finishes in the preconcentrator 3-1 and just can open needle-valve 8, make argon gas compression gas tank 4, preconcentrator 3-1, chromatography column 5-2, microplasma detecting device 5-3 communicates, gas in the argon gas compression gas tank 4 oppositely purges preconcentrator 3-1, the VOCs that desorption is got off flows into chromatography column 5-2 with current-carrying gas to be separated, the component gas of Fen Liing enters microplasma detecting device 5-3 subsequently, VOCs component molecule is excited by the metastable particle in the microplasma detecting device arc chamber, the characteristic light signal that ground state is sent is returned in transition, the optical signals lens that send focus on and are coupled to optical fiber and are transferred to spectrometer again and detect processing, and demonstrate signal processing results and store data by computer, realize the fast detecting to VOCs in the tidal air accordingly.
Claims (10)
1. expiratory air on-line preconcentration gas chromatography method for quick is characterized in that may further comprise the steps:
(1) expiratory air is blown into breath and selects gatherer, select to collect the alveolar gas in the expiratory air;
(2) alveolar gas of selecting to collect is sent into preconcentrator, utilize the VOCs in the adsorbents adsorb alveolar air in the preconcentrator, waste gas is discharged;
(3) the heating preconcentrator makes the VOCs desorption that is enriched on the adsorbent;
(4) microplasma work gas is fed the microplasma detecting device and make microplasma work gas form microplasma under high voltage in arc chamber, this microplasma is made up of metastable atom, ion, electronics, and integral body is electric neutrality;
(5) sweep adsorbent in the preconcentrator with the carrier gas blowback, the VOCs that pyrolysis is analysed get off enters chromatography column with carrier gas, carries out component and separates;
(6) separate the VOCs component gas that obtains through chromatography column and enter the microplasma detecting device with carrier gas, above-mentioned VOCs component gas molecule enters behind the microplasma detecting device arc chamber by existing high-octane metastable particle wherein and excites, and transition is subsequently returned ground state and sent the characteristic light signal;
(7) utilize lens that the characteristic light signal focus that each component molecular transition of VOCs sends is coupled to optical fiber, detect processing by Optical Fiber Transmission to spectrometer, and handle and the storage data by computer, realize the fast detecting to VOCs in the expiratory air.
2. expiratory air on-line preconcentration gas chromatography method for quick according to claim 1 is characterized in that the alveolar gas that described gas selects gatherer to select to collect is the expiratory air behind the discharge dead volume pneumatosis in the expiratory air.
3. expiratory air on-line preconcentration gas chromatography method for quick according to claim 2 is characterized in that the alveolar gas that described gas selects gatherer to select to collect is the gas of last 350ml in the expiratory air.
4. expiratory air on-line preconcentration gas chromatography method for quick according to claim 1, it is characterized in that described alveolar gas in preconcentrator through the fixing VOCs in the alveolar air of at least one section adsorption section absorption that is constituted by interlayer and adsorbent.
5. expiratory air on-line preconcentration gas chromatography method for quick according to claim 4 is characterized in that if in the preconcentrator adsorption section that contains more than two sections is arranged, then the adsorbent type difference of each adsorption section.
6. expiratory air on-line preconcentration gas chromatography method for quick according to claim 5 is characterized in that described alveolar gas mode by physisorption in preconcentrator is fixed on VOCs on the adsorbent surface.
7. according to the described expiratory air on-line preconcentration of one of claim 1 to 6 gas chromatography method for quick, it is characterized in that described gas as VOCs carrier gas and work gas is argon gas, helium or nitrogen.
8. expiratory air on-line preconcentration gas chromatography method for quick according to claim 7 is characterized in that described gas source as VOCs carrier gas and work gas is compression gas tank gas.
9. according to the described expiratory air on-line preconcentration of one of claim 1 to 6 gas chromatography method for quick, it is characterized in that entering chromatography column with carrier gas by described VOCs, to carry out the operating temperature that component separates be 20～40 ℃.
10. according to the described expiratory air on-line preconcentration of one of claim 1 to 6 gas chromatography method for quick, it is characterized in that separating each component gas of VOCs obtain through chromatography column enters the microplasma detecting device successively in the mode of pulse, each component gas of VOCs is excited by high-octane metastable particle in the microplasma detecting device, and transition is returned ground state and sent the characteristic light signal.
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| CN104007208A (en) * | 2014-06-25 | 2014-08-27 | 南通永康检测技术有限公司 | Method for rapidly detecting nitrogen, absorbed by atoms, in expiratory air |
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| CN114544837A (en) * | 2022-01-13 | 2022-05-27 | 黄山嘉讯环境检测技术服务有限公司 | System and method for detecting content of benzene series in air and sampling tube |
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